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[PMID]: | 26265587 |
[Au] Autor: | Velasco-Rodríguez D; Alonso-Domínguez JM; González-Fernández FA; Villarrubia J; Sopeña M; Abalo L; Ropero P; Martínez-Nieto J; de la Fuente Gonzalo F; Cava F |
[Ad] Endereço: | Department of Haematology, Laboratorio Central de la Comunidad de Madrid, Madrid, Spain Programa de Doctorado de Investigación en Ciencias Médico-Quirúrgicas, Universidad Complutense de Madrid, Madrid, Spain Department of Haematology, Hospital Ramón y Cajal, Madrid, Spain. |
[Ti] Título: | Reticulocyte parameters of delta beta thalassaemia trait, beta thalassaemia trait and iron deficiency anaemia. |
[So] Source: | J Clin Pathol;69(2):149-54, 2016 Feb. | [Is] ISSN: | 1472-4146 |
[Cp] País de publicação: | England |
[La] Idioma: | eng |
[Ab] Resumo: | AIMS: To analyse the differences in reticulocyte indices between delta beta thalassaemia trait (δß-TT), beta thalassaemia trait (ß-TT) and iron deficiency anaemia (IDA), and to correlate those differences with the physiopathological features of these three types of microcytoses. METHODS: We performed a descriptive study of 428 samples (43 δß-TT, 179 ß-TT and 206 IDA) that were run on Advia 2120 analyser (Siemens). The following reticulocyte indices were assessed: absolute reticulocyte count (ARC), percentage of reticulocytes, mean corpuscular volume of reticulocytes (MCVr), haemoglobin content of reticulocytes (CHr), mean corpuscular haemoglobin concentration of reticulocytes, red blood cell distribution width of reticulocytes (RDWr), haemoglobin distribution width of reticulocytes (HDWr) and reticulocyte subpopulations based on their fluorescence according to mRNA (low (L-R), medium (M-R) and high (H-R)), MCV ratio and MCHC ratio. Correlation between fetal haemoglobin (HbF) and RDWr in patients with thalassaemia was evaluated. RESULTS: RDWr was significantly higher in δß-TT compared with ß-TT (15.03% vs 13.82%, p<0.001), and so were HDWr (3.65% vs 3.27%, p<0.001), CHr (23.68 vs 22.66 pg, p<0.001) and MCVr (88.3 vs 85.5 fL, p<0.001). A good correlation was observed between HbF and RDWr (r=0.551, p<0.001). IDA subjects have more immature reticulocytes, but less ARC than ß-TT, suggesting a certain degree of inefficient erythropoiesis in IDA in comparison with ß-TT. CONCLUSIONS: Previously described differences between δß-TT, ß-TT and IDA in the corpuscular indices of mature red blood cell can also be observed in reticulocytes. The degree of anisocytosis in reticulocytes from patients with thalassaemia is correlated with HbF. |
[Mh] Termos MeSH primário: |
Anemia Ferropriva/sangue Reticulócitos Talassemia beta/sangue Talassemia delta/sangue
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[Mh] Termos MeSH secundário: |
Anemia Ferropriva/diagnóstico Biomarcadores/sangue Contagem de Eritrócitos Índices de Eritrócitos Hemoglobinas/análise Seres Humanos Valor Preditivo dos Testes Reticulócitos/metabolismo Reticulócitos/patologia Talassemia beta/diagnóstico Talassemia delta/diagnóstico
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[Pt] Tipo de publicação: | COMPARATIVE STUDY; JOURNAL ARTICLE |
[Nm] Nome de substância:
| 0 (Biomarkers); 0 (Hemoglobins) |
[Em] Mês de entrada: | 1606 |
[Cu] Atualização por classe: | 160122 |
[Lr] Data última revisão:
| 160122 |
[Sb] Subgrupo de revista: | AIM; IM |
[Da] Data de entrada para processamento: | 150813 |
[St] Status: | MEDLINE |
[do] DOI: | 10.1136/jclinpath-2015-203034 |
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