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[PMID]: | 27774621 |
[Au] Autor: | Schulz U; Reil A; Kiefel V; Bux J; Moog R |
[Ad] Endereço: | DRK Blood Service North-East, Cottbus, Germany. |
[Ti] Título: | Evaluation of a new microbeads assay for granulocyte antibody detection. |
[So] Source: | Transfusion;57(1):70-81, 2017 01. | [Is] ISSN: | 1537-2995 |
[Cp] País de publicação: | United States |
[La] Idioma: | eng |
[Ab] Resumo: | BACKGROUND: To reduce the risk of transfusion-associated acute lung injury (TRALI), a high number of plasma donors were tested for human leukocyte antigen (HLA) and human neutrophil antigen (HNA) antibodies. For HNA antibody detection, the gold standard is a combination of the granulocyte immunofluorescence test (GIFT) and the granulocyte agglutination test (GAT). However, these tests are not suitable for a high-throughput of samples. STUDY DESIGN AND METHODS: To evaluate the new generation of the LABScreen MULTI assay (One Lambda, Inc.), which has special new beads for all the known HNA specificities, including HNA-3a, 97 sera samples containing well-defined HNA antibodies were used. For background testing, we used 91 samples from plasma donors previously identified by GAT, GIFT, and the monoclonal antibody-specific immobilization of granulocyte antigens (MAIGA) assay. RESULTS: Compared with previous tests, the new LABScreen MULTI assay was highly specific for the HNA-1a, HNA-1b, HNA-2, and HNA-3a antibody specificities required to prevent TRALI. Ninety-eight percent of the HNA-1a, HNA-1b, and HNA-2 antibodies could be detected as true positive; and 90% of the HNA-3a antibodies were recognized correctly as positive. False-positive reactions were identified in 5.5% of samples that previously tested negative. CONCLUSION: The detection of HNA-3a antibody specificities could be integrated into the new LABScreen MULTI assay; however, we detected only 90%. In addition, we detected further HNA antibodies, such as HNA-1c, HNA-1d, and some HNA-3b and HNA-4a antibodies. The new generation of LABScreen MULTI is a great step toward feasible high-throughput testing for HNA antibodies. Nevertheless, GIFT and GAT remain the gold-standard methods for the differentiation of rare and currently unknown HNA specificities. |
[Mh] Termos MeSH primário: |
Autoanticorpos/sangue Isoantígenos/sangue Microesferas
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[Mh] Termos MeSH secundário: |
Lesão Pulmonar Aguda/sangue Lesão Pulmonar Aguda/etiologia Lesão Pulmonar Aguda/prevenção & controle Testes de Aglutinação/métodos Anticorpos Monoclonais/química Feminino Técnica Direta de Fluorescência para Anticorpo/métodos Seres Humanos Masculino Reação Transfusional
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[Pt] Tipo de publicação: | EVALUATION STUDIES; JOURNAL ARTICLE |
[Nm] Nome de substância:
| 0 (Antibodies, Monoclonal); 0 (Autoantibodies); 0 (Isoantigens) |
[Em] Mês de entrada: | 1706 |
[Cu] Atualização por classe: | 180302 |
[Lr] Data última revisão:
| 180302 |
[Sb] Subgrupo de revista: | IM |
[Da] Data de entrada para processamento: | 161025 |
[St] Status: | MEDLINE |
[do] DOI: | 10.1111/trf.13878 |
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