Base de dados : MEDLINE
Pesquisa : C17.800.174.255.400.225 [Categoria DeCS]
Referências encontradas : 780 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 78 ir para página                         

  1 / 780 MEDLINE  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29183759
[Au] Autor:Kim HJ; Lee E; Lee M; Ahn S; Kim J; Liu J; Jin SH; Ha J; Bae IH; Lee TR; Noh M
[Ad] Endereço:Basic Research and Innovation Division, AmorePacific Corporation R&D Center, Yongin, Gyeounggi-do 17074, Republic of Korea.
[Ti] Título:Phosphodiesterase 4B plays a role in benzophenone-3-induced phototoxicity in normal human keratinocytes.
[So] Source:Toxicol Appl Pharmacol;338:174-181, 2018 01 01.
[Is] ISSN:1096-0333
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Benzophenone-3 (BP-3), which is extensively used in organic sunscreen, has phototoxic potential in human skin. Phosphodiesterase 4B (PDE4B) has a well-established role in inflammatory responses in immune cells. Currently, it is unknown if PDE4B is associated with BP-3-induced phototoxicity in normal human keratinocytes (NHKs). We found that BP-3 significantly increased PDE4B expression in ultraviolet B (UVB)-irradiated NHKs. Notably, BP-8, a sunscreen agent that shares the 2-hydroxy-4-methoxyphenyl methanone moiety with BP-3, also upregulated PDE4B expression in NHKs. Upon UVB irradiation, BP-3 upregulated the expression of pro-inflammatory factors, such as prostaglandin endoperoxide synthase 2, tumor necrosis factor α, interleukin 8, and S100A7, and downregulated the level of cornified envelope associated proteins, which are important in the development of the epidermal permeability barrier. The additive effects of UVB-activated BP-3 on the expression of both pro-inflammatory mediators and cornified envelope associated proteins were antagonized by treatment with the PDE4 inhibitor rolipram. The BP-3 and UVB co-stimulation-induced PDE4B upregulation and its association with the upregulation of pro-inflammatory mediators and the downregulation of epidermal differentiation markers were confirmed in a reconstituted three dimensional human epidermis model. Therefore, PDE4B has a role in the mechanism of BP-3-induced phototoxicity.
[Mh] Termos MeSH primário: Benzofenonas/toxicidade
Nucleotídeo Cíclico Fosfodiesterase do Tipo 4/fisiologia
Dermatite Fototóxica/etiologia
Queratinócitos/efeitos dos fármacos
[Mh] Termos MeSH secundário: AMP Cíclico/fisiologia
Nucleotídeo Cíclico Fosfodiesterase do Tipo 4/genética
Dinoprostona/biossíntese
Seres Humanos
Interleucina-8/biossíntese
Fator de Necrose Tumoral alfa/biossíntese
Raios Ultravioleta
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Benzophenones); 0 (Interleukin-8); 0 (Tumor Necrosis Factor-alpha); 95OOS7VE0Y (oxybenzone); E0399OZS9N (Cyclic AMP); EC 3.1.4.17 (Cyclic Nucleotide Phosphodiesterases, Type 4); K7Q1JQR04M (Dinoprostone)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180213
[Lr] Data última revisão:
180213
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171130
[St] Status:MEDLINE


  2 / 780 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28698097
[Au] Autor:Kato M; Ohtake H; Sato H; Seto Y; Onoue S
[Ad] Endereço:Department of Pharmacokinetics and Pharmacodynamics, School of Pharmaceutical Sciences, University of Shizuoka, 52-1 Yada, Suruga-ku, Shizuoka 422-8526, Japan.
[Ti] Título:Enzymatic reactive oxygen species assay to evaluate phototoxic risk of metabolites.
[So] Source:Toxicol Lett;278:59-65, 2017 Aug 15.
[Is] ISSN:1879-3169
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The present study aimed to verify the feasibility of an enzymatic reactive oxygen species (eROS) assay to evaluate the phototoxic risk of compounds after their metabolization. The eROS assay was designed based on the combined use of an in vitro drug metabolism system and a ROS assay. The incubation time of compounds with human hepatic S9 fractions was optimized with the use of fenofibrate (FF), a typical phototoxicant with metabolite-related phototoxicity, and the reproducibility and robustness of the eROS assay were examined using FF. The eROS assay was applied to 12 phototoxic compounds, including 7 phototoxicants with metabolite-related phototoxicity, to clarify the assay performance. According to the eROS data on singlet oxygen generation from FF and metabolic conversion profiles of FF and fenofibric acid, the incubation time of chemicals with human hepatic S9-mix was determined to be 4min. The singlet oxygen-based evaluation system in the eROS assay was found to be acceptable as a high-throughput assay because of its favorable intra-/inter-day reproducibility (coefficient of variation: ca. 8%) and robustness (Z'-factor: 0.23). Singlet oxygen data on phototoxicants with phototoxic metabolites tended to exceed 120% of control, suggesting the feasibility of the eROS assay to evaluate metabolite-related phototoxic potentials. However, further data accumulation is still needed to improve the assay performance because the eROS assay provided false predictions for some compounds. The present eROS assay may be applicable in part for evaluating the phototoxic risk of drug candidates after their metabolization in the early stage of drug discovery.
[Mh] Termos MeSH primário: Bioensaio
Dermatite Fototóxica/etiologia
Fenofibrato/toxicidade
Fígado/efeitos dos fármacos
Processos Fotoquímicos
Oxigênio Singlete/metabolismo
Testes de Toxicidade/métodos
[Mh] Termos MeSH secundário: Biomarcadores/metabolismo
Biotransformação
Dermatite Fototóxica/metabolismo
Relação Dose-Resposta a Droga
Estudos de Viabilidade
Fenofibrato/metabolismo
Fenofibrato/efeitos da radiação
Seres Humanos
Fígado/enzimologia
Reprodutibilidade dos Testes
Medição de Risco
Fatores de Tempo
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biomarkers); 17778-80-2 (Singlet Oxygen); U202363UOS (Fenofibrate)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170821
[Lr] Data última revisão:
170821
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170713
[St] Status:MEDLINE


  3 / 780 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28661494
[Au] Autor:Laissue PP; Alghamdi RA; Tomancak P; Reynaud EG; Shroff H
[Ad] Endereço:School of Biological Sciences, University of Essex, Colchester, UK.
[Ti] Título:Assessing phototoxicity in live fluorescence imaging.
[So] Source:Nat Methods;14(7):657-661, 2017 Jun 29.
[Is] ISSN:1548-7105
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Are the answers to biological questions obtained via live fluorescence microscopy substantially affected by phototoxicity? Although a single set of standards for assessing phototoxicity cannot exist owing to the breadth of samples and experimental questions associated with biological imaging, we need quantitative, practical assessments and reporting standards to ensure that imaging has a minimal impact on observed biological processes and sample health. Here we discuss the problem of phototoxicity in biology and suggest guidelines to improve its reporting and assessment.
[Mh] Termos MeSH primário: Proliferação Celular/efeitos da radiação
Dano ao DNA
Dermatite Fototóxica/etiologia
Luz
Microscopia de Fluorescência/métodos
[Mh] Termos MeSH secundário: Animais
Cercopithecus aethiops
Dermatite Fototóxica/genética
Dermatite Fototóxica/patologia
Radicais Livres/metabolismo
Luz/efeitos adversos
Células Vero
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Free Radicals)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170814
[Lr] Data última revisão:
170814
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170630
[St] Status:MEDLINE
[do] DOI:10.1038/nmeth.4344


  4 / 780 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28476927
[Au] Autor:Kiser PD; Zhang J; Badiee M; Kinoshita J; Peachey NS; Tochtrop GP; Palczewski K
[Ad] Endereço:Department of Pharmacology, School of Medicine (P.D.K., J.Z., K.P.), Department of Chemistry (M.B., G.P.T.), Case Western Reserve University, Cleveland, Ohio; Research Service, Louis Stokes Cleveland Veterans Affairs Medical Center, Cleveland, Ohio (P.D.K., N.S.P.); Cole Eye Institute, Cleveland Cli
[Ti] Título:Rational Tuning of Visual Cycle Modulator Pharmacodynamics.
[So] Source:J Pharmacol Exp Ther;362(1):131-145, 2017 Jul.
[Is] ISSN:1521-0103
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Modulators of the visual cycle have been developed for treatment of various retinal disorders. These agents were designed to inhibit retinoid isomerase [retinal pigment epithelium-specific 65 kDa protein (RPE65)], the rate-limiting enzyme of the visual cycle, based on the idea that attenuation of visual pigment regeneration could reduce formation of toxic retinal conjugates. Of these agents, certain ones that contain primary amine groups can also reversibly form retinaldehyde Schiff base adducts, which contributes to their retinal protective activity. Direct inhibition of RPE65 as a therapeutic strategy is complicated by adverse effects resulting from slowed chromophore regeneration, whereas effective retinal sequestration can require high drug doses with potential off-target effects. We hypothesized that the RPE65-emixustat crystal structure could help guide the design of retinaldehyde-sequestering agents with varying degrees of RPE65 inhibitory activity. We found that addition of an isopropyl group to the central phenyl ring of emixustat and related compounds resulted in agents effectively lacking in vitro retinoid isomerase inhibitory activity, whereas substitution of the terminal 6-membered ring with branched moieties capable of stronger RPE65 interaction potentiated inhibition. The isopropyl derivative series produced discernible visual cycle suppression in vivo, albeit much less potently than compounds with a high affinity for the RPE65 active site. These agents were distributed into the retina and formed Schiff base adducts with retinaldehyde. Except for one compound [3-amino-1-(3-isopropyl-5-((2,6,6-trimethylcyclohex-1-en-1-yl)methoxy)phenyl)propan-1-ol (MB-007)], these agents conferred protection against retinal phototoxicity, suggesting that both direct RPE65 inhibition and retinal sequestration are mechanisms of potential therapeutic relevance.
[Mh] Termos MeSH primário: Visão Ocular/efeitos dos fármacos
cis-trans-Isomerases/antagonistas & inibidores
[Mh] Termos MeSH secundário: Transportadores de Cassetes de Ligação de ATP/genética
Transportadores de Cassetes de Ligação de ATP/metabolismo
Oxirredutases do Álcool/genética
Oxirredutases do Álcool/metabolismo
Animais
Sítios de Ligação
Bovinos
Dermatite Fototóxica/prevenção & controle
Feminino
Cinética
Masculino
Camundongos
Camundongos Endogâmicos C57BL
Microssomos/enzimologia
Modelos Moleculares
Epitélio Pigmentado Ocular/efeitos dos fármacos
Regeneração/efeitos dos fármacos
Doenças Retinianas/prevenção & controle
Epitélio Pigmentado da Retina/efeitos dos fármacos
Bases de Schiff/química
cis-trans-Isomerases/química
cis-trans-Isomerases/genética
cis-trans-Isomerases/isolamento & purificação
cis-trans-Isomerases/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Abca4 protein, mouse); 0 (Schiff Bases); EC 1.1.- (Alcohol Oxidoreductases); EC 1.1.1.105 (Rdh8 protein, mouse); EC 3.1.1.64 (retinoid isomerohydrolase); EC 5.2.- (cis-trans-Isomerases)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170714
[Lr] Data última revisão:
170714
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170507
[St] Status:MEDLINE
[do] DOI:10.1124/jpet.117.240721


  5 / 780 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28418140
[Au] Autor:Ibbotson SH
[Ad] Endereço:Photobiology Unit, University of Dundee, Ninewells Hospital and Medical School, Dundee, DD1 9SY, U.K.
[Ti] Título:Shedding light on drug photosensitivity reactions.
[So] Source:Br J Dermatol;176(4):850-851, 2017 04.
[Is] ISSN:1365-2133
[Cp] País de publicação:England
[La] Idioma:eng
[Mh] Termos MeSH primário: Dermatite Fototóxica
Transtornos de Fotossensibilidade
[Mh] Termos MeSH secundário: Seres Humanos
Luz
[Pt] Tipo de publicação:JOURNAL ARTICLE; COMMENT
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171107
[Lr] Data última revisão:
171107
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170419
[St] Status:MEDLINE
[do] DOI:10.1111/bjd.15449


  6 / 780 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
[PMID]:28403266
[Au] Autor:Stevenson ML; Karen JK; Hale EK
[Ti] Título:Laser-Assisted Photodynamic Therapy: Two Novel Protocols for Enhanced Treatment Results.
[So] Source:J Drugs Dermatol;16(4):329-331, 2017 Apr 01.
[Is] ISSN:1545-9616
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:

Photodynamic therapy (PDT) uses a topical photosensitizing agent which is activated by a light source to cause destruction of specific cells. Commonly used for the treatment of actinic keratoses and photodamage, PDT can also be used for other conditions including acne and sebaceous hyperplasia. Here we report our experience with two treatment protocols. The first protocol utilizes laser assisted delivery of topical 5-aminolevulinic acid for enhanced efficacy of blue light photodynamic therapy in the treatment of actinic keratoses and photodamage. The second protocol utilizes red light photodynamic therapy followed by pulsed dye laser to effectively target sebaceous glands in patients with extensive sebaceous hyperplasia.

J Drugs Dermatol. 2017;16(4):329-331.

.
[Mh] Termos MeSH primário: Ácido Aminolevulínico/uso terapêutico
Dermatite Fototóxica/terapia
Ceratose Actínica/terapia
Lasers de Corante/uso terapêutico
Terapia com Luz de Baixa Intensidade/métodos
Fotoquimioterapia/métodos
Fármacos Fotossensibilizantes/uso terapêutico
Glândulas Sebáceas/efeitos da radiação
[Mh] Termos MeSH secundário: Administração Cutânea
Idoso
Protocolos Clínicos
Feminino
Seres Humanos
Lasers Semicondutores
Masculino
Meia-Idade
Glândulas Sebáceas/patologia
Resultado do Tratamento
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Photosensitizing Agents); 88755TAZ87 (Aminolevulinic Acid)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170914
[Lr] Data última revisão:
170914
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170414
[St] Status:MEDLINE


  7 / 780 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28321041
[Au] Autor:Yonezawa Y; Miyashita T; Ashizawa H; Hashimoto K; Nejishima H; Ogawa H
[Ad] Endereço:Pharmacokinetics and Safety Department Drug Research Center Kaken Pharmaceutical Co., Ltd.
[Ti] Título:Evaluation of a general toxicity study incorporating phototoxicity assessments in Sprague-Dawley rats.
[So] Source:J Toxicol Sci;42(2):145-157, 2017.
[Is] ISSN:1880-3989
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:Previously, we showed that phototoxicity assessments in Sprague-Dawley (SD) rats can detect phototoxic potential to the same degree as those in guinea pigs. In this study, we examined whether phototoxicity assessments can be incorporated into general toxicology studies, using SD rats. Three phototoxic compounds were tested. Acridine and 8-methoxypsoralen (8-MOP) were transdermally administered, and 8-MOP and lomefloxacin were orally administered. The animals were allocated to three groups for each compound: single-dose, repeated-dose, and repeated-dose plus toxicokinetics (TK). The single-dose group was irradiated with UV-A and UV-B after a single administration of the drug. The repeated-dose and TK groups were irradiated after 8 days of repeated administration of the drug. Blood samples were also collected from the TK group on days 1 and 7 after administration. The phototoxic compounds resulted in skin reactions in all the groups, with no difference in the degree of skin reaction among the three groups. In the TK measurements, all of the phototoxic compounds were detected in the plasma samples, and the irradiation timing was close to the T . These results indicate that phototoxic potential could be evaluated in the TK group, and phototoxicity assessments could be incorporated into general toxicology studies. This reduces the number of studies and animals required, thus shortening the research and development period, and supporting the 3Rs principle of animal experiments. The study also provides information regarding appropriate irradiation timings, differences between the sexes, and dose-response, in turn enabling the phototoxic risk of the compounds to be clearly evaluated.
[Mh] Termos MeSH primário: Acridinas/toxicidade
Fluoroquinolonas/toxicidade
Metoxaleno/toxicidade
Fármacos Fotossensibilizantes/toxicidade
Testes de Toxicidade/métodos
[Mh] Termos MeSH secundário: Acridinas/análise
Acridinas/farmacocinética
Administração Cutânea
Administração Oral
Animais
Dermatite Fototóxica
Fluoroquinolonas/sangue
Fluoroquinolonas/farmacocinética
Masculino
Metoxaleno/sangue
Metoxaleno/farmacocinética
Fármacos Fotossensibilizantes/sangue
Fármacos Fotossensibilizantes/farmacocinética
Ratos Sprague-Dawley
Pele/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Acridines); 0 (Fluoroquinolones); 0 (Photosensitizing Agents); L6BR2WJD8V (lomefloxacin); U4VJ29L7BQ (Methoxsalen)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170801
[Lr] Data última revisão:
170801
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170322
[St] Status:MEDLINE
[do] DOI:10.2131/jts.42.145


  8 / 780 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28258860
[Au] Autor:Shang E; Niu J; Li Y; Zhou Y; Crittenden JC
[Ad] Endereço:State Key Laboratory of Water Environment Simulation, School of Environment, Beijing Normal University, Beijing 100875, PR China.
[Ti] Título:Comparative toxicity of Cd, Mo, and W sulphide nanomaterials toward E. coli under UV irradiation.
[So] Source:Environ Pollut;224:606-614, 2017 May.
[Is] ISSN:1873-6424
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:In this study, the phototoxicity of cadmium sulfide (CdS), molybdenum disulfide (MoS ), and tungsten disulfide (WS ) nanoparticles (NPs) toward Escherichia coli (E. coli) under UV irradiation (365 nm) was investigated. At the same mass concentration of NPs, the toxicity of three NPs decreased in the order of CdS > MoS > WS . For example, the death rates of E. coli exposed to 50 mg/L CdS, MoS , and WS were 96.7%, 38.5%, and 31.2%, respectively. Transmission electron microscope and laser scanning confocal microscope images of E. coli exposed to three NPs showed the damage of cell walls and release of intracellular components. The CdS-treated cell wall was more extensively damaged than those of MoS -treated and WS -treated bacteria. WS and MoS generated superoxide radical (O ), singlet oxygen ( O ), and hydroxyl radical under UV irradiation, CdS produced only O and O . CdS and WS released ions under UV irradiation, while MoS did not. Reactive oxygen species (ROS) generation and toxic ion release jointly resulted in the antibacterial activities of CdS and WS . ROS generation was the dominant toxic mechanism of MoS toward the bacteria. This study highlighted the importance of considering the hazardous effect of sulfide NPs after their release into natural waters under light irradiation condition.
[Mh] Termos MeSH primário: Cádmio/toxicidade
Escherichia coli/efeitos dos fármacos
Molibdênio/toxicidade
Nanopartículas/toxicidade
Sulfetos/toxicidade
Tungstênio/toxicidade
Raios Ultravioleta
[Mh] Termos MeSH secundário: Dermatite Fototóxica
Luz
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Sulfides); 00BH33GNGH (Cadmium); 81AH48963U (Molybdenum); V9306CXO6G (Tungsten)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170522
[Lr] Data última revisão:
170522
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170305
[St] Status:MEDLINE


  9 / 780 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28192168
[Au] Autor:González MT; Fumagalli F; Benevenuto CG; da Silva Emery F; Gaspar LR
[Ad] Endereço:Department of Pharmaceutical Sciences, School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, 14040-903 Ribeirão Preto, SP, Brazil. Electronic address: mtpaezg@gmail.com.
[Ti] Título:Novel benzophenone-3 derivatives with promising potential as UV filters: Relationship between structure, photoprotective potential and phototoxicity.
[So] Source:Eur J Pharm Sci;101:200-210, 2017 Apr 01.
[Is] ISSN:1879-0720
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Benzophenone-3 (BP-3) is a UV filter with absorption at the UVB and UVA wavelengths which has not been extensively studied in experiments involving its absorbing effects and toxicity. We synthetized four BP-3 derivatives and characterized their photoprotective potential by UV absorption and photodegradation, their phototoxicity potential by 3T3 Neutral Red Uptake (3T3 NRU PT) and their photoreactivity by the reactive oxygen species (ROS) assay. The UV absorption, photodegradation, phototoxicity and photoreactivity of the four BP-3 derivatives (BP-3 carbonate, BP-3 carbazole, BP-3 phenylamine and BP-3 methoxy-phenylamine) were evaluated and compared to those of BP-3. Results showed that all derivatives were photostable, except BP-3 carbonate, which did not absorb in the UVA range. BP-3 phenylamine and BP-3 methoxy-phenylamine were considered non-phototoxic and weakly photoreactive in the ROS assay, while the carbazole derivative was considered phototoxic and non-photoreactive due to its rigid structure. The UV spectra of BP-3 carbonate, BP-3 phenylamine and BP-3 methoxy-phenylamine showed the influence of hydrogen bonding on their UV absorption. Based on these results, we concluded that BP-3 phenylamine and BP-3 methoxy-phenylamine could be promising UVA filters.
[Mh] Termos MeSH primário: Benzofenonas/química
Dermatite Fototóxica/etiologia
Protetores Solares/química
[Mh] Termos MeSH secundário: Bioensaio/métodos
Ligações de Hidrogênio
Vermelho Neutro/química
Espécies Reativas de Oxigênio/química
Raios Ultravioleta
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Benzophenones); 0 (Reactive Oxygen Species); 0 (Sunscreening Agents); 261QK3SSBH (Neutral Red); 95OOS7VE0Y (oxybenzone)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170621
[Lr] Data última revisão:
170621
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170214
[St] Status:MEDLINE


  10 / 780 MEDLINE  
              first record previous record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28082110
[Au] Autor:Mori T; Higashi K; Nakano T; Ando S; Kuwahara A; Suzuki N; Saito K
[Ad] Endereço:Environmental Health Science Laboratory, Sumitomo Chemical Co., Ltd., Osaka 554-8558, Japan.
[Ti] Título:Novel phototoxicity assay using human embryonic stem cell-derived retinal pigment epithelial cells.
[So] Source:Toxicology;378:1-9, 2017 Mar 01.
[Is] ISSN:1879-3185
[Cp] País de publicação:Ireland
[La] Idioma:eng
[Ab] Resumo:Some chemicals are harmful in to light-exposed tissues such as skin and eyes. The 3T3 Neutral Red Uptake Phototoxicity Test has been validated and adopted by the Organization of Economic and Community Development (OECD) as a method of evaluating chemical phototoxicity using mouse 3T3 fibroblasts. However, the high rate of false positive results associated with this test eventually led to increased laboratory animal usage. Although the eye is vulnerable to light damage because of constant exposure to environmental radiation, few approaches are available to predict ocular phototoxicity in humans. Here, we propose a tier one test that identifies the potential ocular phototoxicity of chemical substances. Using a three-dimensional culture technique, human embryonic stem cells (hESCs) were differentiated to retinal pigment epithelial cell (RPE) precursors. The precursors after prolonged treatment with FBS formed a uniform hexagonal lattice of cells with well-developed tight junctions and time-dependent elevation of melanin content and RPE maturation marker levels. Hierarchical clustering of gene transcripts revealed that hESC-derived RPEs were very similar to tissue-derived adult RPEs. Interestingly, there were a high percentage of chemicals eliciting a positive response in 3T3 cells and negative in hESC-derived RPEs under the experimental conditions used in the phototoxicity test. The response to treatment of hESC-derived RPEs with these negative chemicals became positive at a higher dose of UVA irradiation; however, the biological responses to these chemicals differed between the two cells. Taken together, we conclude that hESC-derived RPEs are novel tool for future toxicological and mechanistic studies of ocular phototoxicity in humans.
[Mh] Termos MeSH primário: Bioensaio/métodos
Dermatite Fototóxica
Células Epiteliais/efeitos dos fármacos
Células Epiteliais/efeitos da radiação
Células-Tronco Embrionárias Humanas/citologia
Epitélio Pigmentado da Retina/citologia
[Mh] Termos MeSH secundário: Células 3T3
Animais
Diferenciação Celular
Células Cultivadas
Técnicas de Cocultura
Células Epiteliais/metabolismo
Expressão Gênica
Seres Humanos
Melaninas/metabolismo
Camundongos
Raios Ultravioleta
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Melanins)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170512
[Lr] Data última revisão:
170512
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170114
[St] Status:MEDLINE



página 1 de 78 ir para página                         
   


Refinar a pesquisa
  Base de dados : MEDLINE Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde