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[PMID]:29246346
[Au] Autor:Leickly FE; Kloepfer KM; Slaven JE; Vitalpur G
[Ad] Endereço:Section of Pediatric Pulmonology, Allergy, and Sleep Medicine, Department of Pediatrics, Indiana University School of Medicine and Riley Hospital for Children, Indiana University Health, Carmel, IN. Electronic address: fleickly@iu.edu.
[Ti] Título:Peanut Allergy: An Epidemiologic Analysis of a Large Database.
[So] Source:J Pediatr;192:223-228.e1, 2018 Jan.
[Is] ISSN:1097-6833
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:OBJECTIVE: To confirm new observations on peanut allergy and answer current concerns that families and healthcare providers have about peanut allergy. STUDY DESIGN: Children who presented with a story of peanut allergy or peanut sensitization were asked to participate in a registry, which allowed an analysis focused on questions that a food allergy support group had about children with peanut allergy or sensitization. RESULTS: A total of 1070 children were entered into the registry over 5 years. Two-thirds had a reaction to peanut. Children with peanut allergy were predominantly male (63%), white (78%), and with private health insurance (80%). Most reactions involved the skin (55%) and anaphylaxis occurred in 35%. The median age of a reaction was 1 year old. Atopic dermatitis was noted in 60% and asthma in 41%. Additional food allergy was noted in 58%. When second exposures occurred 28% had a more severe reaction. Skin test size did not differentiate the type of a reaction and children with anaphylaxis had slightly higher specific IgE levels. Severe reactions with inadvertent exposure in children who were peanut sensitized was rare (<1%). CONCLUSIONS: The strategies for peanut allergy prevention and treatment have evolved. The data obtained in this large registry can answer many questions that families and healthcare providers have during this transition.
[Mh] Termos MeSH primário: Hipersensibilidade a Amendoim/epidemiologia
[Mh] Termos MeSH secundário: Adolescente
Criança
Pré-Escolar
Bases de Dados Factuais
Feminino
Seguimentos
Seres Humanos
Indiana/epidemiologia
Lactente
Masculino
Hipersensibilidade a Amendoim/complicações
Hipersensibilidade a Amendoim/diagnóstico
Hipersensibilidade a Amendoim/terapia
Sistema de Registros
Fatores de Risco
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171226
[Lr] Data última revisão:
171226
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:171217
[St] Status:MEDLINE


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[PMID]:28972089
[Au] Autor:Bednar KJ; Shanina E; Ballet R; Connors EP; Duan S; Juan J; Arlian BM; Kulis MD; Butcher EC; Fung-Leung WP; Rao TS; Paulson JC; Macauley MS
[Ad] Endereço:Immunology Team, Janssen Research and Development, LLC, Raritan, NJ 08869.
[Ti] Título:Human CD22 Inhibits Murine B Cell Receptor Activation in a Human CD22 Transgenic Mouse Model.
[So] Source:J Immunol;199(9):3116-3128, 2017 Nov 01.
[Is] ISSN:1550-6606
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:CD22, a sialic acid-binding Ig-type lectin (Siglec) family member, is an inhibitory coreceptor of the BCR with established roles in health and disease. The restricted expression pattern of CD22 on B cells and most B cell lymphomas has made CD22 a therapeutic target for B cell-mediated diseases. Models to better understand how in vivo targeting of CD22 translates to human disease are needed. In this article, we report the development of a transgenic mouse expressing human CD22 (hCD22) in B cells and assess its ability to functionally substitute for murine CD22 (mCD22) for regulation of BCR signaling, Ab responses, homing, and tolerance. Expression of hCD22 on transgenic murine B cells is comparable to expression on human primary B cells, and it colocalizes with mCD22 on the cell surface. Murine B cells expressing only hCD22 have identical calcium (Ca ) flux responses to anti-IgM as mCD22-expressing wild-type B cells. Furthermore, hCD22 transgenic mice on an mCD22 background have restored levels of marginal zone B cells and Ab responses compared with deficiencies observed in CD22 mice. Consistent with these observations, hCD22 transgenic mice develop normal humoral responses in a peanut allergy oral sensitization model. Homing of B cells to Peyer's patches was partially rescued by expression of hCD22 compared with CD22 B cells, although not to wild-type levels. Notably, Siglec-engaging antigenic liposomes formulated with an hCD22 ligand were shown to prevent B cell activation, increase cell death, and induce tolerance in vivo. This hCD22 transgenic mouse will be a valuable model for investigating the function of hCD22 and preclinical studies targeting hCD22.
[Mh] Termos MeSH primário: Linfócitos B/imunologia
Hipersensibilidade a Amendoim/imunologia
Nódulos Linfáticos Agregados/imunologia
Receptores de Antígenos de Linfócitos B/imunologia
Lectina 2 Semelhante a Ig de Ligação ao Ácido Siálico/imunologia
Transdução de Sinais/imunologia
[Mh] Termos MeSH secundário: Animais
Linfócitos B/patologia
Modelos Animais de Doenças
Seres Humanos
Ativação Linfocitária/genética
Camundongos
Camundongos Transgênicos
Hipersensibilidade a Amendoim/genética
Hipersensibilidade a Amendoim/patologia
Nódulos Linfáticos Agregados/patologia
Receptores de Antígenos de Linfócitos B/genética
Lectina 2 Semelhante a Ig de Ligação ao Ácido Siálico/genética
Transdução de Sinais/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (CD22 protein, human); 0 (Cd22 protein, mouse); 0 (Receptors, Antigen, B-Cell); 0 (Sialic Acid Binding Ig-like Lectin 2)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171111
[Lr] Data última revisão:
171111
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:171004
[St] Status:MEDLINE
[do] DOI:10.4049/jimmunol.1700898


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[PMID]:28950267
[Au] Autor:Schocker F; Scharf A; Kull S; Jappe U
[Ad] Endereço:Division of Clinical and Molecular Allergology, Research Center Borstel, Priority Research Area Asthma and Allergy, Airway Research Center North (ARCN), German Center for Lung Research (DZL), Borstel, Germany.
[Ti] Título:Detection of the Peanut Allergens Ara h 2 and Ara h 6 in Human Breast Milk: Development of 2 Sensitive and Specific Sandwich ELISA Assays.
[So] Source:Int Arch Allergy Immunol;174(1):17-25, 2017.
[Is] ISSN:1423-0097
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Little is known about breast milk as a vehicle for tolerance development or sensitization to peanuts very early in life. Thus, well-characterized and highly sensitive detection systems for the reliable determination of peanut allergens in breast milk are mandatory. METHODS: For the quantification of the marker allergens Ara h 2 and Ara h 6 in the low nanogram per milliliter range in breast milk samples of a German cohort, sensitive and highly specific sandwich ELISAs were optimized and validated. RESULTS: The Ara h 2 ELISA revealed a limit of detection (LOD) of 1.3 ng Ara h 2/mL and a quantification range of 2.3-250 ng/mL, the Ara h 6 ELISA showed an LOD of 0.7 ng/mL and a working range of 1.1-14.4 ng/mL. The assays showed no relevant cross-reactivity against other potentially cross-reactive legume, seed, and tree nut extracts (<0.01%, except for Ara h 1 in the Ara h 2 ELISA <0.1%). Ara h 2 was detectable in breast milk samples from 14/40 (35%) of the participants in concentrations from 2.3 to 184 ng/mL, Ara h 6 appeared in 9/40 (22.5%) of the lactating mothers between 1.1 and 9.7 ng/mL, and 1 highly positive sample with 79 ng/mL. Both allergens appeared at the same time points, but Ara h 6 in lower concentrations than Ara h 2. CONCLUSIONS: Sensitive and specific diagnostic tools for the determination of Ara h 2 and Ara h 6 in human breast milk were established. The kinetics of secreted Ara h 2 and Ara h 6 seem to be similar but with a difference in concentration. Follow-up investigations on their tolerogenic or sensitizing properties in breast milk become now accessible.
[Mh] Termos MeSH primário: Albuminas 2S de Plantas/análise
Antígenos de Plantas/análise
Ensaio de Imunoadsorção Enzimática/métodos
Glicoproteínas/análise
Leite Humano/química
Hipersensibilidade a Amendoim/diagnóstico
Proteínas de Plantas/imunologia
[Mh] Termos MeSH secundário: Alérgenos/análise
Arachis/imunologia
Reações Cruzadas/imunologia
Feminino
Seres Humanos
Lactação/fisiologia
Limite de Detecção
Hipersensibilidade a Amendoim/prevenção & controle
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (2S Albumins, Plant); 0 (Allergens); 0 (Antigens, Plant); 0 (Ara h 2 allergen, Arachis hypogaea); 0 (Ara h 6 allergen, Arachis hypogaea); 0 (Glycoproteins); 0 (Plant Proteins)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
171031
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170927
[St] Status:MEDLINE
[do] DOI:10.1159/000479388


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[PMID]:28930512
[Au] Autor:Jones SM; Burks AW
[Ad] Endereço:From the Department of Pediatrics, University of Arkansas for Medical Sciences and Arkansas Children's Hospital, Little Rock (S.M.J.); and the Department of Pediatrics, University of North Carolina, Chapel Hill (A.W.B.).
[Ti] Título:Food Allergy.
[So] Source:N Engl J Med;377(12):1168-1176, 2017 09 21.
[Is] ISSN:1533-4406
[Cp] País de publicação:United States
[La] Idioma:eng
[Mh] Termos MeSH primário: Dessensibilização Imunológica/métodos
Hipersensibilidade Alimentar/terapia
[Mh] Termos MeSH secundário: Adolescente
Anafilaxia/diagnóstico
Anafilaxia/etiologia
Epinefrina/uso terapêutico
Hipersensibilidade Alimentar/complicações
Hipersensibilidade Alimentar/diagnóstico
Hipersensibilidade Alimentar/prevenção & controle
Seres Humanos
Imunoterapia
Masculino
Educação de Pacientes como Assunto
Hipersensibilidade a Amendoim/prevenção & controle
Fatores de Risco
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; REVIEW
[Nm] Nome de substância:
YKH834O4BH (Epinephrine)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170926
[Lr] Data última revisão:
170926
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170921
[St] Status:MEDLINE
[do] DOI:10.1056/NEJMcp1611971


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[PMID]:28890021
[Au] Autor:Valcour A; Jones JE; Lidholm J; Borres MP; Hamilton RG
[Ad] Endereço:Laboratory Corporation of America, Burlington, North Carolina. Electronic address: valcoua@labcorp.com.
[Ti] Título:Sensitization profiles to peanut allergens across the United States.
[So] Source:Ann Allergy Asthma Immunol;119(3):262-266.e1, 2017 Sep.
[Is] ISSN:1534-4436
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Measurement of IgE antibody to peanut components can aid in the prediction of allergic responses the food. OBJECTIVE: To investigate the association between patient demographics (age, location) and allergic sensitization to peanut components across the United States. METHODS: Serum samples from 12,155 individuals with peanut extract specific IgE levels of 0.35 kUA/L or higher were analyzed for IgE antibodies to Ara h 1, 2, 3, 8, and 9 by ImmunoCAP. RESULTS: Among this population of peanut sensitized individuals, 79.1% of children (<3 years old) were sensitized to one or more peanut storage proteins (Ara h 1, 2, and/or 3), in contrast to 64.2% of adolescents (12-15 years old) and 22.1% of adults (>20 years old). Although sensitization was more prevalent to Ara h 2 than to the other storage proteins, a sizable fraction of patients were sensitized to Ara h 1 and/or 3 but not to Ara h 2 (eg, 13% of children <3 years old). Moreover, 9.6% of children, 10.2% of adolescents, and 10.5% of adults were sensitized to Ara h 9, whereas 2.4% of children, 49.4% of adolescents, and 42.9% of adults produced IgE to Ara h 8 (pathogenesis-related protein 10). Sensitization to Ara h 8 alone was markedly higher in the Northeastern United States relative to other regions of the country. CONCLUSION: We conclude that sensitization to individual peanut components is highly dependent on age and geographic location. Given that a severe allergic reaction to peanut is unlikely in individuals with isolated sensitization to Ara h 8, a sizable fraction of patients, in particular adolescents and adults, may be at lower risk than anticipated based only on demonstration of sensitization to whole peanut extract.
[Mh] Termos MeSH primário: Alérgenos/imunologia
Antígenos de Plantas/imunologia
Arachis/imunologia
Hipersensibilidade a Amendoim/imunologia
Proteínas de Plantas/imunologia
[Mh] Termos MeSH secundário: Adolescente
Adulto
Criança
Pré-Escolar
Seres Humanos
Imunoglobulina E/sangue
Imunoglobulina E/imunologia
Lactente
Recém-Nascido
Meia-Idade
Hipersensibilidade a Amendoim/sangue
Estados Unidos
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Allergens); 0 (Antigens, Plant); 0 (Plant Proteins); 37341-29-0 (Immunoglobulin E)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170918
[Lr] Data última revisão:
170918
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170912
[St] Status:MEDLINE


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[PMID]:28850948
[Au] Autor:Marco-Martín G; La Rotta Hernández A; Vázquez de la Torre M; Higaki Y; Zubeldia JM; Baeza ML
[Ad] Endereço:Allergy Service and Experimental Medicine Unit, Hospital General Universitario Gregorio Marañón, Madrid, Spain.
[Ti] Título:Differences in the Anaphylactic Response between C3H/HeOuJ and BALB/c Mice.
[So] Source:Int Arch Allergy Immunol;173(4):204-212, 2017.
[Is] ISSN:1423-0097
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Anaphylaxis is a severe and potentially lethal allergic reaction whose incidence is increasing. Murine models can elucidate the underlying mechanisms and pave the way for appropriate therapeutic options. However, differences in strains and protocols hamper comparisons of data between researchers. We performed a parallel study of clinical and immune responses with 2 strains of mice, BALB/c and C3H/HeOuJ, in an allergen-induced systemic anaphylaxis protocol. Both strains have been widely used in allergy models, although they have not been compared in an intraperitoneal systemic model. METHODS: Groups of 5-week-old female BALB/c and C3H/HeOuJ mice were intraperitoneally sensitized with peanut in the presence of adjuvants. Specific immunoglobulin (sIg) G1, sIgG2a, sIgE, total IgE, histamine release, and specific stimulated splenocyte cytokines, interleukin (IL)-4, IL-5, IL-10, IL-12, IL-13, and interferon (IFN)-γ, were assessed. At week 6, mice were intraperitoneally challenged with peanut. Anaphylaxis was evaluated by recognition of clinical symptoms and changes in body temperature. RESULTS: All peanut-sensitized mice induced sIg and developed anaphylactic symptoms upon challenge. Nonetheless, the C3H/HeOuJ strain demonstrated earlier and persistently higher sIgG1 and sIgG2a production, elevated sIgE, and more severe clinical symptoms and histamine release than the BALB/c strain. In contrast, BALB/c exhibited higher release of IL-4, IL-5, IL-10, IL-13, and IFN-γ. CONCLUSIONS: Both models are suitable for studying anaphylaxis. Consequently, they could be used in research on the pathogenesis and therapy of anaphylaxis. However, according to the type of study performed, differences in the specific clinical, humoral, and cellular responses to antigens have to be considered.
[Mh] Termos MeSH primário: Anafilaxia/imunologia
Citocinas/imunologia
Imunoglobulinas/imunologia
Hipersensibilidade a Amendoim/imunologia
[Mh] Termos MeSH secundário: Animais
Arachis/imunologia
Feminino
Liberação de Histamina
Camundongos Endogâmicos BALB C
Camundongos Endogâmicos C3H
Especificidade da Espécie
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cytokines); 0 (Immunoglobulins)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171003
[Lr] Data última revisão:
171003
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170830
[St] Status:MEDLINE
[do] DOI:10.1159/000478983


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[PMID]:28800361
[Au] Autor:Zhao L; Zhao L; Zhang B; Robotham JM; Roux KH; Tang H
[Ad] Endereço:Institute of Health Sciences, Anhui University, Hefei, Anhui, PR China.
[Ti] Título:Identification of a common Ara h 3 epitope recognized by both the capture and the detection monoclonal antibodies in an ELISA detection kit.
[So] Source:PLoS One;12(8):e0182935, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Allergy to peanuts has become a common and severe problem, especially in westernized countries. In this study, we evaluated the target and epitope specificity of the capture and detection mouse monoclonal antibodies (mAbs) used in a commercial peanut allergen detection platform. We first identified the target of these antibodies as Ara h 3 and then used an overlapping peptide array of Ara h 3 to determine the antibody-binding epitopes. Further amino acids critical for the binding via alanine substitutions at individual amino acid residues within the epitope were mapped. Finally, inhibition ELISA and inhibition immunoblotting using a recombinant Ara h 3 protein were performed to confirm these results. Surprisingly, the capture and detection mAbs showed identical binding characteristics and were presumed to represent two isolates of the same clone, a notion supported by both isoelectric focusing electrophoresis and Liquid chromatography-mass spectrometry experiments. The simultaneous binding of a pair of identical mAbs to an individual allergen such as Ara h3 is attributed to the multivalency of the analyte and has implications for developing diagnostic assays for additional multimeric allergens.
[Mh] Termos MeSH primário: Anticorpos Monoclonais/química
Antígenos de Plantas/química
Arachis/química
Epitopos/análise
Hipersensibilidade a Amendoim/diagnóstico
Proteínas de Plantas/química
Proteínas de Armazenamento de Sementes/química
[Mh] Termos MeSH secundário: Alanina/química
Alanina/genética
Alanina/imunologia
Alérgenos/química
Alérgenos/imunologia
Sequência de Aminoácidos
Substituição de Aminoácidos
Animais
Anticorpos Monoclonais/biossíntese
Anticorpos Monoclonais/isolamento & purificação
Antígenos de Plantas/genética
Antígenos de Plantas/imunologia
Arachis/imunologia
Ensaio de Imunoadsorção Enzimática/normas
Mapeamento de Epitopos
Epitopos/química
Epitopos/imunologia
Expressão Gênica
Seres Humanos
Camundongos
Modelos Moleculares
Hipersensibilidade a Amendoim/imunologia
Proteínas de Plantas/genética
Proteínas de Plantas/imunologia
Análise Serial de Proteínas
Multimerização Proteica
Estrutura Secundária de Proteína
Kit de Reagentes para Diagnóstico
Proteínas Recombinantes/química
Proteínas Recombinantes/genética
Proteínas Recombinantes/imunologia
Proteínas de Armazenamento de Sementes/genética
Proteínas de Armazenamento de Sementes/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Allergens); 0 (Antibodies, Monoclonal); 0 (Antigens, Plant); 0 (Epitopes); 0 (Plant Proteins); 0 (Reagent Kits, Diagnostic); 0 (Recombinant Proteins); 0 (Seed Storage Proteins); 0 (allergen Ara h3); OF5P57N2ZX (Alanine)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171004
[Lr] Data última revisão:
171004
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170812
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0182935


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[PMID]:28691223
[Au] Autor:Lawson K; Bahnson HT; Brittain E; Sever M; Du Toit G; Lack G; Keet C; Greenhawt M; Fleischer D; Chan ES; Venter C; Stukus D; Gupta R; Spergel J
[Ad] Endereço:Rho Federal Systems Division, Chapel Hill, NC, USA.
[Ti] Título:Letter of response to Greenhawt et al. 'LEAPing Through the Looking Glass: Secondary Analysis of the Effect of Skin Test Size and Age of Introduction on Peanut Tolerance after Early Peanut Introduction'.
[So] Source:Allergy;72(8):1267-1271, 2017 08.
[Is] ISSN:1398-9995
[Cp] País de publicação:Denmark
[La] Idioma:eng
[Mh] Termos MeSH primário: Arachis/imunologia
Hipersensibilidade a Amendoim
[Mh] Termos MeSH secundário: Seres Humanos
Tolerância Imunológica
Imunoglobulina E
Testes Cutâneos
[Pt] Tipo de publicação:LETTER; COMMENT
[Nm] Nome de substância:
37341-29-0 (Immunoglobulin E)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171023
[Lr] Data última revisão:
171023
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170711
[St] Status:MEDLINE
[do] DOI:10.1111/all.13127


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[PMID]:28666018
[Au] Autor:Hammerschmidt-Kamper C; Biljes D; Merches K; Steiner I; Daldrup T; Bol-Schoenmakers M; Pieters RHH; Esser C
[Ad] Endereço:IUF - Leibniz Research Institute for Environmental Medicine, Düsseldorf, Germany.
[Ti] Título:Indole-3-carbinol, a plant nutrient and AhR-Ligand precursor, supports oral tolerance against OVA and improves peanut allergy symptoms in mice.
[So] Source:PLoS One;12(6):e0180321, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In general, dietary antigens are tolerated by the gut associated immune system. Impairment of this so-called oral tolerance is a serious health risk. We have previously shown that activation of the ligand-dependent transcription factor aryl hydrocarbon receptor (AhR) by the environmental pollutant 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) affects both oral tolerance and food allergy. In this study, we determine whether a common plant-derived, dietary AhR-ligand modulates oral tolerance as well. We therefore fed mice with indole-3-carbinole (I3C), an AhR ligand that is abundant in cruciferous plants. We show that several I3C metabolites were detectable in the serum after feeding, including the high-affinity ligand 3,3´-diindolylmethane (DIM). I3C feeding robustly induced the AhR-target gene CYP4501A1 in the intestine; I3C feeding also induced the aldh1 gene, whose product catalyzes the formation of retinoic acid (RA), an inducer of regulatory T cells. We then measured parameters indicating oral tolerance and severity of peanut-induced food allergy. In contrast to the tolerance-breaking effect of TCDD, feeding mice with chow containing 2 g/kg I3C lowered the serum anti-ovalbumin IgG1 response in an experimental oral tolerance protocol. Moreover, I3C feeding attenuated symptoms of peanut allergy. In conclusion, the dietary compound I3C can positively influence a vital immune function of the gut.
[Mh] Termos MeSH primário: Indóis/farmacologia
Ovalbumina/farmacologia
Hipersensibilidade a Amendoim/prevenção & controle
Receptores de Hidrocarboneto Arílico/efeitos dos fármacos
[Mh] Termos MeSH secundário: Administração Oral
Animais
Ensaio de Imunoadsorção Enzimática
Indóis/administração & dosagem
Camundongos
Camundongos Endogâmicos C57BL
Receptores de Hidrocarboneto Arílico/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Indoles); 0 (Receptors, Aryl Hydrocarbon); 9006-59-1 (Ovalbumin); C11E72455F (indole-3-carbinol)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171006
[Lr] Data última revisão:
171006
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170701
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0180321


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[PMID]:28634114
[Au] Autor:Di Stasio L; Picariello G; Mongiello M; Nocerino R; Berni Canani R; Bavaro S; Monaci L; Ferranti P; Mamone G
[Ad] Endereço:Institute of Food Sciences, CNR, Avellino, Italy; Department of Agriculture, University of Naples Federico II, Portici (Naples), Italy.
[Ti] Título:Peanut digestome: Identification of digestion resistant IgE binding peptides.
[So] Source:Food Chem Toxicol;107(Pt A):88-98, 2017 Sep.
[Is] ISSN:1873-6351
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Stability to proteolytic degradation in the digestive tract is considered a general feature shared by most food allergens. Current digestibility models exclusively utilize purified allergen proteins, neglecting the relevant effects of matrix that occur for foodstuff systems. In the present study, we investigated digestion stability of the major peanut allergens directly in the natural matrix using an in vitro static model that simulates the gastrointestinal digestion including the oral, gastric, duodenal and intestinal (brush border membrane enzymes) phases. Immunogenicity was evaluated by Western Blot using N=8 pooled sera of peanut allergic pediatric subjects. Immunoreactive, large-sized and fragments of Ara h 2, Ara h 6 and Ara h 3 survived hydrolysis as assessed by SDS-PAGE. Smaller resistant peptides mainly arising from Ara h 3 and also Ara h 1 were detected and further identified by LC-high resolution-MS/MS. RP-HPLC purification followed by dot-blot analysis and MS/MS-based identification demonstrated that stable IgE-binding peptides derived from Ara h 3. These results provide a more realistic picture of the potentially allergenic determinants of peanuts that survived the human digestion, taking into account the role of the food matrix, which may significantly affect gastrointestinal breakdown of peanut allergens.
[Mh] Termos MeSH primário: Arachis/química
Imunoglobulina E/imunologia
Hipersensibilidade a Amendoim/imunologia
Peptídeos/imunologia
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Antígenos de Plantas/química
Antígenos de Plantas/genética
Antígenos de Plantas/imunologia
Antígenos de Plantas/metabolismo
Arachis/genética
Arachis/imunologia
Arachis/metabolismo
Digestão
Eletroforese em Gel de Poliacrilamida
Seres Humanos
Dados de Sequência Molecular
Hipersensibilidade a Amendoim/metabolismo
Mapeamento de Peptídeos
Peptídeos/química
Peptídeos/genética
Espectrometria de Massas em Tandem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antigens, Plant); 0 (Peptides); 37341-29-0 (Immunoglobulin E)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170829
[Lr] Data última revisão:
170829
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170622
[St] Status:MEDLINE



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