Base de dados : MEDLINE
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[PMID]:29381760
[Au] Autor:Yatabe T; More SJ; Geoghegan F; McManus C; Hill AE; Martínez-López B
[Ad] Endereço:Center for Animal Disease Modeling and Surveillance (CADMS), Department of Medicine & Epidemiology, School Veterinary Medicine, University of California, Davis, California, United States of America.
[Ti] Título:Can biosecurity and local network properties predict pathogen species richness in the salmonid industry?
[So] Source:PLoS One;13(1):e0191680, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Salmonid farming in Ireland is mostly organic, which implies limited disease treatment options. This highlights the importance of biosecurity for preventing the introduction and spread of infectious agents. Similarly, the effect of local network properties on infection spread processes has rarely been evaluated. In this paper, we characterized the biosecurity of salmonid farms in Ireland using a survey, and then developed a score for benchmarking the disease risk of salmonid farms. The usefulness and validity of this score, together with farm indegree (dichotomized as ≤ 1 or > 1), were assessed through generalized Poisson regression models, in which the modeled outcome was pathogen richness, defined here as the number of different diseases affecting a farm during a year. Seawater salmon (SW salmon) farms had the highest biosecurity scores with a median (interquartile range) of 82.3 (5.4), followed by freshwater salmon (FW salmon) with 75.2 (8.2), and freshwater trout (FW trout) farms with 74.8 (4.5). For FW salmon and trout farms, the top ranked model (in terms of leave-one-out information criteria, looic) was the null model (looic = 46.1). For SW salmon farms, the best ranking model was the full model with both predictors and their interaction (looic = 33.3). Farms with a higher biosecurity score were associated with lower pathogen richness, and farms with indegree > 1 (i.e. more than one fish supplier) were associated with increased pathogen richness. The effect of the interaction between these variables was also important, showing an antagonistic effect. This would indicate that biosecurity effectiveness is achieved through a broader perspective on the subject, which includes a minimization in the number of suppliers and hence in the possibilities for infection to enter a farm. The work presented here could be used to elaborate indicators of a farm's disease risk based on its biosecurity score and indegree, to inform risk-based disease surveillance and control strategies for private and public stakeholders.
[Mh] Termos MeSH primário: Doenças dos Peixes/prevenção & controle
Salmonidae/microbiologia
Medidas de Segurança
[Mh] Termos MeSH secundário: Animais
Doenças dos Peixes/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180131
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0191680


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[PMID]:29458492
[Au] Autor:Liu Y; Rao Q; Tu J; Zhang J; Huang M; Hu B; Lin Q; Luo T
[Ad] Endereço:1​Institute of Quality Standards and Testing Technology for Agro-Products, Fujian Academy of Agricultural Sciences, Fuzhou 350003, PR China.
[Ti] Título:Acinetobacter piscicola sp. nov., isolated from diseased farmed Murray cod (Maccullochella peelii peelii).
[So] Source:Int J Syst Evol Microbiol;68(3):905-910, 2018 Mar.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A taxonomic study was carried out on strain LW15 , which was isolated from the external lesions of diseased farmed Murray cod (Maccullochella peelii peelii) from an intensive culture pond. Cells of strain LW15 were Gram-negative, facultative-anaerobic, non-motile, and both coccobacillus- and bacillus-shaped. Growth was observed at NaCl concentrations of 0-2 % (w/v) (optimum, 0 %), 4-32 °C (optimum, 25-28 °C) and pH 5.0-9.0 (optimum, 7.0). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain LW15 was affiliated to the genus Acinetobacter, showing the highest similarity to Acinetobacter guillouiae CIP 63.46 (97.7 %) and other Acinetobacter species with validly published names (93.5-97.6 %). Whole-genome sequencing and phylogeny reconstruction based on a core set of 1061 Acinetobacter genes indicated that strain LW15 was most closely related to the clade formed by A. guillouiae CIP 63.46 and Acinetobacter bereziniae CIP 70.12 and distantly related to any of the described species of genus Acinetobacter. Furthermore, strain LW15 could be distinguished from all known Acinetobacter species by its ability to assimilate ß-alanine and l-arginine, but not d-glucose. The principal fatty acids were C18 : 1ω9c, C16 : 0 and C16 : 1ω7c/C16 : 1ω6c. The major respiratory quinone was Q-9. Polar lipids of strain LW15 comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, four phospholipids, aminolipid and two unknown lipids. Based on its phenotypic and genotypic data, strain LW15 represents a novel species of the genus Acinetobacter, for which the name Acinetobacterpiscicola sp. nov. is proposed. The type strain is LW15 (=MCCC 1K03337 =CICC 24241 =KCTC 62134 =JCM 32101 ).
[Mh] Termos MeSH primário: Acinetobacter/classificação
Doenças dos Peixes/microbiologia
Peixes/microbiologia
Filogenia
[Mh] Termos MeSH secundário: Acinetobacter/genética
Acinetobacter/isolamento & purificação
Animais
Aquicultura
Técnicas de Tipagem Bacteriana
Composição de Bases
China
DNA Bacteriano/genética
Ácidos Graxos/química
Hibridização de Ácido Nucleico
Fosfolipídeos/química
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
Vitamina K 2/análogos & derivados
Vitamina K 2/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Fatty Acids); 0 (Phospholipids); 0 (RNA, Ribosomal, 16S); 11032-49-8 (Vitamin K 2); 523-39-7 (menaquinone 9)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180308
[Lr] Data última revisão:
180308
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180221
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.002608


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[PMID]:29277002
[Au] Autor:Irigoitia MM; Braicovich PE; Lanfranchi AL; Farber MD; Timi JT
[Ad] Endereço:Laboratorio de Ictioparasitología, Instituto de Investigaciones Marinas y Costeras (IIMyC), Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Mar del Plata - Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Funes 3350, 7600 Mar del Plata, Argentina. Electronic ad
[Ti] Título:Distribution of anisakid nematodes parasitizing rajiform skates under commercial exploitation in the Southwestern Atlantic.
[So] Source:Int J Food Microbiol;267:20-28, 2018 Feb 21.
[Is] ISSN:1879-3460
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:In order to evaluate the infestation by anisakids present in elasmobranchs and their distribution in the Argentine Sea, this study was carried at a regional scale with the following aims: 1) to identify those anisakid species present in skates under exploitation; 2) to characterize quantitatively these infestations and 3) to determine those factors driving the variability in parasite burdens across skate species. A total of 351 skates, belonging to 3 species (218 Sympterygia bonapartii, 86 Zearaja chilensis and 47 Atlantoraja castelnaui) and from different localities of the Argentine Sea were examined for anisakids. Parasites were found in the stomach wall at high prevalence in some samples. Based on morphology and mtDNA cox2 sequences analyses (from 24 larval worms), specimens were identified as Anisakis berlandi, A. pegreffii and Pseudoterranova cattani; the last two known as potentially pathogenic for humans. Differential distribution patterns were observed across parasite and hosts species. In general, fish caught in southern and deeper waters exhibited higher loads of Anisakis spp., whereas infestation levels by P. cattani increase in larger skates. Taking into account that the mere presence of worms or their antigens in fish meat can provoke allergic responses, information on distribution of parasites and their variability is essential for the implementation of food safety practices.
[Mh] Termos MeSH primário: Anisaquíase/parasitologia
Anisakis/fisiologia
Doenças dos Peixes/parasitologia
Raias/parasitologia
[Mh] Termos MeSH secundário: Distribuição Animal
Animais
Anisaquíase/epidemiologia
Anisakis/genética
Oceano Atlântico/epidemiologia
DNA Mitocondrial/genética
Complexo IV da Cadeia de Transporte de Elétrons/genética
Doenças dos Peixes/epidemiologia
Pesqueiros
Larva
Carga Parasitária
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Mitochondrial); EC 1.9.3.1 (Electron Transport Complex IV)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180305
[Lr] Data última revisão:
180305
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171226
[St] Status:MEDLINE


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[PMID]:29274208
[Au] Autor:Ardalan NM; Al-Hashimi SF; Garhy M; Hamdi SAH; Al Ghamdi A; Morsy K
[Ad] Endereço:Department of Biology, College of Science for Woman, University of Baghdad, Mohamed Al-Qasim St., 10071, Baghdad, Iraq
[Ti] Título:The light microscopy and ultrastructural characteristics of Myxobolus naffari (Myxosporea, Myxobolidae) infecting the Nile carp Labeo niloticus (Cyprinidae) and its histological impact
[So] Source:Ann Parasitol;63(3):159-165, 2017.
[Is] ISSN:2299-0631
[Cp] País de publicação:Poland
[La] Idioma:eng
[Ab] Resumo:During a survey of myxosporean parasites infecting freshwater fishes from the River Nile at Giza Governorates, Egypt between March and September 2016, nine out of 30 specimens of the Nile carp Labeo niloticus (Cyprinidae) were found to be naturally infected with Myxobolus naffari (Myxobolidae). Small macroscopic plasmodia appeared embedded in the host gill tissue accompanied with fusion of the gill epithelia, and atrophy was observed at the site of infection. The host reaction was manifested by the encapsulation of the plasmodia with a thick layer of connective tissue. The plasmodia appeared as white, elongated rods between gill filaments with an intensity ranging from three to eight cysts/fish. The average dimensions of plasmodia were 1.2­2.0 (1.8 ± 0.2) mm long × 0.4­0.7 (0.6 ± 0.2) mm wide. The spores were oval, reaching 9.56­11.2 (10.2 ± 0.2) µm long and 6.5­7.7 (7.0 ± 0.4) µm wide with two equal-sized polar capsules regularly arranged at the anterior pole of each spore. They were 4.51­5.5 (5.1 ± 0.4) µm in length and 1.5­2.0 (1.7 ± 0.2) µm in width. Histological, semi-thin sections were taken through parasite plasmodia and transmission electron microscopic examination of ultrathin sections was performed to describe the developmental stages of the recorded parasite within the host fish.
[Mh] Termos MeSH primário: Cyprinidae/parasitologia
Doenças dos Peixes/parasitologia
Myxobolus/ultraestrutura
Doenças Parasitárias em Animais/parasitologia
[Mh] Termos MeSH secundário: Animais
Egito/epidemiologia
Doenças dos Peixes/epidemiologia
Doenças Parasitárias em Animais/epidemiologia
Rios
Esporos/ultraestrutura
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180305
[Lr] Data última revisão:
180305
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171224
[St] Status:MEDLINE
[do] DOI:10.17420/ap6303.101


  5 / 14816 MEDLINE  
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[PMID]:29256425
[Au] Autor:de Buron I; Hill-Spanik KM; Haselden L; Atkinson SD; Hallett SL; Arnott SA
[Ad] Endereço:Department of Biology, College of Charleston, Charleston, South Carolina 29412, USA.
[Ti] Título:Infection dynamics of Kudoa inornata (Cnidaria: Myxosporea) in spotted seatrout Cynoscion nebulosus (Teleostei: Sciaenidae).
[So] Source:Dis Aquat Organ;127(1):29-40, 2017 Dec 19.
[Is] ISSN:0177-5103
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Kudoa inornata is a myxosporean parasite that develops in the somatic muscle of spotted seatrout Cynoscion nebulosus, an economically and ecologically important fish in estuaries and harbors in southeastern North America. In South Carolina (SC), USA, over 90% of wild adult spotted seatrout are infected. To inform potential mitigation strategies, we conducted 3 experiments using naïve sentinel seatrout and infectious stages of K. inornata naturally present in raw water from Charleston Harbor, SC, to determine (1) if K. inornata infection follows a seasonal pattern, and (2) how long it takes for myxospores to develop in fish muscle. Infection by K. inornata was determined by visual detection of myxospores in fish muscle squashes, and any visually negative samples were then assayed for K. inornata ribosomal DNA using novel parasite-specific PCR primers. We observed that K. inornata infection in seatrout followed a seasonal pattern, with high prevalence when water temperature was highest (27-31°C; July-September) and infections that were either covert (at ~13-15°C) or not detected (<13°C) at the lowest water temperatures in January-February. Myxospore development occurred within 476 degree-days, i.e. 2 wk in a typical SC summer. Infection was dependent on fish density, which limited presumptive actinospore dose. Our findings suggest that the life cycle of the parasite may be disrupted by preventing spore-rich seatrout carcasses (e.g. at angler cleaning stations) being thrown back into harbors and estuaries throughout the year.
[Mh] Termos MeSH primário: Cnidários/fisiologia
Doenças dos Peixes/parasitologia
Peixes/parasitologia
Doenças Parasitárias em Animais/parasitologia
[Mh] Termos MeSH secundário: Animais
Aquicultura
Seres Humanos
Espécies Sentinelas
Fatores de Tempo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180305
[Lr] Data última revisão:
180305
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171220
[St] Status:MEDLINE
[do] DOI:10.3354/dao03174


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[PMID]:29256424
[Au] Autor:Bigarré L; Plassiart G; de Boisséson C; Pallandre L; Pozet F; Ledoré Y; Fontaine P; Lieffrig F
[Ad] Endereço:ANSES, Laboratoire Ploufragan-Plouzané, 29280 Plouzané, France.
[Ti] Título:Molecular investigations of outbreaks of Perch perhabdovirus infections in pike-perch.
[So] Source:Dis Aquat Organ;127(1):19-27, 2017 Dec 19.
[Is] ISSN:0177-5103
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:In 2016, a total of 5 massive mortality episodes each affecting hundreds of thousands of pike-perch Sander lucioperca larvae occurred at 2 sites in 2 Western European countries. For each episode, perhabdoviruses related to the perch rhabdovirus (PRV) were detected in samples, using either PCR or cell culture combined with PCR. The sequences of the glycoprotein (g), phosphoprotein (p) and nucleoprotein (n) genes of these samples demonstrated that 2 different genotypes were present at 1 site, each associated with 1 of the 3 episodes. At the other site, a single genotype was associated with the 2 outbreaks. Furthermore, this genotype was strictly identical to 1 genotype involved in the outbreaks of the first site, strongly suggesting a common origin for these 2 viruses. The common origin was confirmed a posteriori because some larvae introduced to both sites had exactly the same geographic origin in Eastern Europe. Taken together, the molecular and epidemiological data suggest that both horizontal and vertical transmission of 2 distinct strains of perhabdoviruses were involved in the various outbreaks affecting pike-perch.
[Mh] Termos MeSH primário: Surtos de Doenças/veterinária
Doenças dos Peixes/virologia
Perciformes/virologia
Infecções por Rhabdoviridae/veterinária
Rhabdoviridae
[Mh] Termos MeSH secundário: Animais
Células Cultivadas
Europa (Continente)/epidemiologia
Doenças dos Peixes/epidemiologia
Larva/virologia
Rhabdoviridae/genética
Infecções por Rhabdoviridae/epidemiologia
Infecções por Rhabdoviridae/virologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180305
[Lr] Data última revisão:
180305
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171220
[St] Status:MEDLINE
[do] DOI:10.3354/dao03177


  7 / 14816 MEDLINE  
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[PMID]:29256279
[Au] Autor:Herczeg D; Sipos D; Dán Á; Loy C; Kallert DM; Eszterbauer E
[Ad] Endereço:1 Institute for Veterinary Medical Research, Centre for Agricultural Research, Hungarian Academy of Sciences , Hungária krt. 21, H-1143 Budapest , Hungary.
[Ti] Título:The effect of dietary immunostimulants on the susceptibility of common carp (Cyprinus carpio) to the white spot parasite, Ichthyophthirius multifiliis.
[So] Source:Acta Vet Hung;65(4):517-530, 2017 12.
[Is] ISSN:0236-6290
[Cp] País de publicação:Hungary
[La] Idioma:eng
[Ab] Resumo:One of the main obstacles in freshwater aquaculture is the parasitic ciliate Ichthyophthirius multifiliis (Ich), the causative agent of white spot disease. The use of immunostimulants as feed additives may be a promising approach to control Ich infection. In the present study, we tested the prophylactic effect of orally administered ß-1,3/1,6-glucan and propolis extract E50 against Ich infection in common carp. In total, 122 fish were separated into three experimental groups fed with a control, 3% ß-glucan and 1% propolis diet for 40 consecutive days, respectively. On day 40, 16 fish per group were individually exposed to Ich theronts and the number of trophonts was counted 5 days post exposure. Relative gene expression of interleukin 1-ß (IL-1-ß) in common carp liver was examined by qPCR. Compared to control, the mean infection intensity was lower in the ß-glucan- and propolis-fed groups; however, the difference was not statistically significant. The relative expression of IL-1-ß significantly decreased in the propolis-fed group at day 10. In the ß-glucan-fed group, a significant IL-1-ß decrease was detected at day 15 compared to control. Although the Ich infection intensity was slightly decreased in both treated groups, and IL-1-ß was moderately down-regulated in the liver of common carp, our results suggest that the applied feeding regime is insufficient to prevent Ich outbreaks in common carp.
[Mh] Termos MeSH primário: Adjuvantes Imunológicos/administração & dosagem
Carpas
Infecções por Cilióforos/veterinária
Cilióforos
Suplementos Nutricionais
Doenças dos Peixes/parasitologia
[Mh] Termos MeSH secundário: Animais
Cilióforos/genética
Cilióforos/isolamento & purificação
Infecções por Cilióforos/parasitologia
Infecções por Cilióforos/prevenção & controle
RNA/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Adjuvants, Immunologic); 63231-63-0 (RNA)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180305
[Lr] Data última revisão:
180305
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171220
[St] Status:MEDLINE
[do] DOI:10.1556/004.2017.050


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[PMID]:29278825
[Au] Autor:Di Azevedo MIN; Iñiguez AM
[Ad] Endereço:LABTRIP, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro 21045-900, Brazil.
[Ti] Título:Nematode parasites of commercially important fish from the southeast coast of Brazil: Morphological and genetic insight.
[So] Source:Int J Food Microbiol;267:29-41, 2018 02 21.
[Is] ISSN:1879-3460
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Studies of nematofauna of teleost fish from the Brazilian coast are relatively scarce and limited to identification based on morphology. The objective of the present study was to determine the diversity and prevalence of nematode parasites in teleost fish from the southeast Atlantic coast of Rio de Janeiro, through morphological, molecular, and ecological approaches. Parasites were collected from sixty specimens each of Genypterus brasiliensis, Micropogonias furnieri, and Mullus argentinae obtained in winters and summers of 2012­2014. Morphological and genetic characterization was conducted using light microscopy and the molecular targets 18S rDNA, ITS1, and mtDNA cox2. Nematodes identified in M. furnieri were Cucculanus genypteri (n = 1575, P = 98.3%) and Hysterothylacium deardorffoverstreetorum (s.l.) (n = 2, P = 3.3%); in G. brasiliensis were Dichelyne (Cucullanellus) sciaenidicola (n = 99, P = 33.3%), Cucculanus pulcherrimus (n = 45, P = 18.3%), Hysterothylacium deardorffoverstreetorum (s.l.) (n = 3, P = 5%), and Anisakis typica (n = 1, P = 1.7%); and, in M. argentinae, were H. deardorffoverstreetorum (s.l.) (n = 146, P = 48.3%), and Procamallanus (Spirocamallanus) halitrophus (n = 4, P = 6.7%). DNA sequence data of C. genypteri, C. pulcherrimus, D. (C.) sciaenidicola, and P. (S.) halitrophus were reported for the first time. New host records are M. argentinae for P. (S.) halitrophus, M. furnieri for A. typica, while H. deardorffoverstreetorum (s.l.) was found in all three fish species. Intestine showed significantly higher intensity than other sites, and no significant seasonal variation in parasitological indices was observed. Hysterothylacium specimens (n = 6) were found in fish muscle, potentially a public health concern.
[Mh] Termos MeSH primário: Doenças dos Peixes/parasitologia
Nematoides/genética
Infecções por Nematoides/parasitologia
Perciformes/parasitologia
[Mh] Termos MeSH secundário: Animais
Brasil
DNA Mitocondrial/genética
DNA Ribossômico/genética
Nematoides/classificação
Nematoides/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Mitochondrial); 0 (DNA, Ribosomal)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180301
[Lr] Data última revisão:
180301
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171227
[St] Status:MEDLINE


  9 / 14816 MEDLINE  
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[PMID]:29339070
[Au] Autor:Wang R; Li Y; Zhou Z; Liu Q; Zeng L; Xiao T
[Ad] Endereço:Hunan Engineering Technology Research Center of Featured Aquatic Resources Utilization, Hunan Agricultural University, Changsha 410128, China; College of Life and Environment Sciences, Hunan University of Arts and Science, Changde, Hunan 415000, China.
[Ti] Título:Involvement of interferon regulatory factor 3 from the barbel chub Squaliobarbus curriculus in the immune response against grass carp reovirus.
[So] Source:Gene;648:5-11, 2018 Mar 30.
[Is] ISSN:1879-0038
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The barbel chub Squaliobarbus curriculus is an important commercial fish species in China, and has shown significant resistance to grass carp reovirus (GCRV). In this study, the cDNA sequence of interferon regulatory factors 3 (IRF3) from Squaliobarbus curriculus, designated as ScIRF3, was cloned, and its effect against GCRV was investigated. The full-length 1837 base pair (bp) cDNA of ScIRF3 contained a complete open reading frame of 1374 bp and encoded a putative polypeptide of 457 amino acid residues. The ScIRF3 protein contained conserved domains, including an N-terminal DNA-binding domain, a C-terminal IRF association domain, and a serine-rich domain. Phylogenetic analysis showed that ScIRF3 was closely clustered with IRF3s from Carassius auratus and Ctenopharyngodon idellus. Quantitative real-time polymerase chain reaction analysis showed that the expression levels of ScIRF3 in Squaliobarbus curriculus were the highest in the spleen and lowest in the muscle. After GCRV infection, expression levels of both ScIRF3 and type I interferon (IFN) were initially up-regulated and subsequently down-regulated in the spleen and intestine. Correlation analysis showed that the expression level of type I IFN is significantly positively correlated with that of ScIRF3 (Pearson correlation coefficient: 0.883, P: 0.004) in the intestine. The expression level of type I IFN was also significantly up-regulated and the GCRV titer was significantly decreased (P < .05) in GCRV-infected ScIRF3-overexpressing Ctenopharyngodon idellus kidney cells. These results indicate that ScIRF3 may play a role in the type I IFN immune response against GCRV in Squaliobarbus curriculus and can also inhibit GCRV replication in Ctenopharyngodon idellus kidney cells.
[Mh] Termos MeSH primário: Cyprinidae/imunologia
Doenças dos Peixes/imunologia
Proteínas de Peixes/imunologia
Fator Regulador 3 de Interferon/imunologia
Reoviridae/imunologia
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Cyprinidae/metabolismo
Cyprinidae/virologia
Doenças dos Peixes/metabolismo
Doenças dos Peixes/virologia
Proteínas de Peixes/classificação
Proteínas de Peixes/metabolismo
Perfilação da Expressão Gênica/métodos
Interações Hospedeiro-Patógeno/imunologia
Fator Regulador 3 de Interferon/genética
Fator Regulador 3 de Interferon/metabolismo
Interferon Tipo I/genética
Interferon Tipo I/imunologia
Interferon Tipo I/metabolismo
Filogenia
Reoviridae/fisiologia
Homologia de Sequência de Aminoácidos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fish Proteins); 0 (Interferon Regulatory Factor-3); 0 (Interferon Type I)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180226
[Lr] Data última revisão:
180226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180118
[St] Status:MEDLINE


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[PMID]:29339067
[Au] Autor:Sun Y; Zhang J; Xiang J
[Ad] Endereço:College of Life Sciences, Hebei University, Baoding, Hebei 071002, China; College of Marine Life and Fisheries, Huaihai Institute of Technology, 59 Cangwu Road, Lianyungang 222005, China.
[Ti] Título:Molecular characterization and function of ß-N-acetylglucosaminidase from ridgetail white prawn Exopalaemon carinicauda.
[So] Source:Gene;648:12-20, 2018 Mar 30.
[Is] ISSN:1879-0038
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Chitin degradation is catalyzed by a two-component chitinolytic enzyme system, chitinase and ß-N-acetylglucosaminidase (NAGase). In this paper, the full-length cDNA sequence encoding NAGase (EcNAG) was obtained from Exopalaemon carinicauda. The deduced amino acid sequence of EcNAG open reading frame (ORF) contained one Glycohydro_20b2 domain and one Glyco_hydro_20 domain. Based on the cDNA sequence, the genomic structure of EcNAG was characterized and it was composed of six exons and five introns. EcNAG mRNA majorly expressed in the hepatopancreas and epidermis. During the molting stages, EcNAG mRNA expression was well-regulated and its expression reached the highest level at the molting stage E. In addition, EcNAG was recombinant expressed in Pichia pastoris and the partial enzymatic characterization of recombinant EcNAG was confirmed. After being challenged with Vibrio parahaemolyticus and Aeromonas hydrophila, the expression of EcNAG was up-regulated significantly at 6 h and reached the peak at 12 h. And then, the expression began to down-regulated and came to the normal level at 72 h. It is helpful to research the relationship between the molt-related hormones and chitinlytic enzymes.
[Mh] Termos MeSH primário: Acetilglucosaminidase/genética
Proteínas de Artrópodes/genética
Muda/genética
Palaemonidae/genética
[Mh] Termos MeSH secundário: Acetilglucosaminidase/classificação
Acetilglucosaminidase/metabolismo
Aeromonas hydrophila/fisiologia
Sequência de Aminoácidos
Animais
Proteínas de Artrópodes/classificação
Proteínas de Artrópodes/metabolismo
Sequência de Bases
Epiderme/crescimento & desenvolvimento
Epiderme/metabolismo
Epiderme/microbiologia
Doenças dos Peixes/genética
Doenças dos Peixes/microbiologia
Perfilação da Expressão Gênica/métodos
Regulação da Expressão Gênica no Desenvolvimento
Regulação Enzimológica da Expressão Gênica
Hepatopâncreas/crescimento & desenvolvimento
Hepatopâncreas/metabolismo
Hepatopâncreas/microbiologia
Palaemonidae/crescimento & desenvolvimento
Palaemonidae/microbiologia
Filogenia
Homologia de Sequência de Aminoácidos
Vibrio parahaemolyticus/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Arthropod Proteins); EC 3.2.1.52 (Acetylglucosaminidase)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180226
[Lr] Data última revisão:
180226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180118
[St] Status:MEDLINE



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