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[PMID]:29408624
[Au] Autor:Wang X; Li W; Xu X; Wang W; He K; Fan H
[Ad] Endereço:Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, National Center for Engineering Research of Veterinary Bio-products, Nanjing 210014, China; College of Veterinary Medicine, Nanjing
[Ti] Título:Phylogenetic analysis of two goat-origin PCV2 isolates in China.
[So] Source:Gene;651:57-61, 2018 Apr 20.
[Is] ISSN:1879-0038
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Complete genome characterization of non-porcine origin Porcine circovirus type 2 (PCV2) was first described in 2014 in China. In the present study, we first identified PCV2 nucleotides in goat samples and the prevalence of PCV2 in goat was 6.15%. However, only two new strains, Goat2014-4 and Goat2014-5, could be completely sequenced. The genome of the strain Goat2014-4, which collected from the goat infected with PPRV, contains 1766 nt; strain Goat2014-5, which originated from a healthy goat, is comprised of 1767 nt. The results showed that they shared the highest nucleotide identity with BDH and the lowest similarity with DK1980PMWSfree strain and they belonged only to genotype PCV2d. Meanwhile, they shared higher homology with porcine-origin PCV2 strains than others. Moreover, a detailed analysis of the capsid amino acid sequences revealed that there were distinct differences for goat2014-4 (708 bp) and goat2014-5 (705 bp); strain Goat2014-4 showed an elongation of two amino acids, and strains Goat2014-5 showed an elongation of one amino acid compared with other reference strains. This is the first report of the genetic analysis of goat-origin PCV2 isolates. It also provides an additional supported evidence for cross-species transmission of PCV2.
[Mh] Termos MeSH primário: Infecções por Circoviridae/veterinária
Circovirus/classificação
Circovirus/isolamento & purificação
Doenças das Cabras/virologia
[Mh] Termos MeSH secundário: Animais
Proteínas do Capsídeo/genética
China
Infecções por Circoviridae/virologia
Frequência do Gene
Genoma Viral
Cabras
Filogenia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Capsid Proteins)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180207
[St] Status:MEDLINE


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[PMID]:29246990
[Ti] Título:Mortality due to pulpy kidney in beef calves and goats.
[So] Source:Vet Rec;181(24):646-650, 2017 12 16.
[Is] ISSN:2042-7670
[Cp] País de publicação:England
[La] Idioma:eng
[Mh] Termos MeSH primário: Doenças dos Animais/epidemiologia
Surtos de Doenças/veterinária
Vigilância de Evento Sentinela/veterinária
[Mh] Termos MeSH secundário: Animais
Doenças das Aves/epidemiologia
Aves
Bovinos
Doenças dos Bovinos/epidemiologia
Doenças dos Bovinos/mortalidade
Feminino
Doenças das Cabras/epidemiologia
Doenças das Cabras/mortalidade
Cabras
Nefropatias/mortalidade
Nefropatias/veterinária
Aves Domésticas
Gravidez
Escócia/epidemiologia
Ovinos
Doenças dos Ovinos/epidemiologia
Suínos
Doenças dos Suínos/epidemiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180307
[Lr] Data última revisão:
180307
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171217
[St] Status:MEDLINE
[do] DOI:10.1136/vr.j5822


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[PMID]:29246991
[Ti] Título: type D enterotoxaemia in cattle, goats and sheep.
[So] Source:Vet Rec;181(24):648-649, 2017 12 16.
[Is] ISSN:2042-7670
[Cp] País de publicação:England
[La] Idioma:eng
[Mh] Termos MeSH primário: Doenças dos Bovinos/diagnóstico
Clostridium perfringens/isolamento & purificação
Enterotoxemia/diagnóstico
Doenças das Cabras/diagnóstico
Doenças dos Ovinos/diagnóstico
[Mh] Termos MeSH secundário: Animais
Toxinas Bacterianas/isolamento & purificação
Bovinos
Doenças dos Bovinos/microbiologia
Clostridium perfringens/classificação
Enterotoxemia/microbiologia
Conteúdo Gastrointestinal
Doenças das Cabras/microbiologia
Cabras
Vigilância de Evento Sentinela/veterinária
Ovinos
Doenças dos Ovinos/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Toxins)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180307
[Lr] Data última revisão:
180307
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171217
[St] Status:MEDLINE
[do] DOI:10.1136/vr.j5823


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[PMID]:29256284
[Au] Autor:Fábián R; Kovács A; Stéger V; Frank K; Egerszegi I; Oláh J; Bodó S
[Ad] Endereço:1 National Agricultural Research and Innovation Centre , Szent-Györgyi Albert u. 4, H-2100 Gödöllo , Hungary.
[Ti] Título:X- and Y-chromosome-specific variants of the amelogenin gene allow non-invasive sex diagnosis for the detection of pseudohermaphrodite goats.
[So] Source:Acta Vet Hung;65(4):500-504, 2017 12.
[Is] ISSN:0236-6290
[Cp] País de publicação:Hungary
[La] Idioma:eng
[Ab] Resumo:The Polled Intersex Syndrome (PIS) is responsible for the absence of horns in homozygous and heterozygous goats causing a female-to-male sex reversal in the homozygous polled genotypic female (XX) goats. A simple and efficient non-invasive method was elaborated to detect the genotypic sex from hair and faecal samples using a pair of primers to amplify the X- and Y-linked alleles of the amelogenin gene. The PCR products were easily distinguishable using agarose gel electrophoresis: we detected an X-specific single band in samples originating from healthy phenotypic females and double (X- and Y-) bands in samples from males. The new PCR method is applicable for diagnosing the sex of PIS-affected animals already as newborn kids, in contrast with the phenotypic findings appearing only after puberty, and thus it may replace the cumbersome chromosome investigations.
[Mh] Termos MeSH primário: Amelogenina/metabolismo
Transtornos do Desenvolvimento Sexual/veterinária
Doenças das Cabras/genética
Cromossomo X/genética
Cromossomo Y/genética
[Mh] Termos MeSH secundário: Amelogenina/genética
Animais
DNA/química
DNA/genética
Transtornos do Desenvolvimento Sexual/diagnóstico
Transtornos do Desenvolvimento Sexual/genética
Fezes/química
Feminino
Regulação da Expressão Gênica
Genótipo
Doenças das Cabras/diagnóstico
Cabras
Cabelo/química
Masculino
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Amelogenin); 9007-49-2 (DNA)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180305
[Lr] Data última revisão:
180305
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171220
[St] Status:MEDLINE
[do] DOI:10.1556/004.2017.047


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[PMID]:29351277
[Au] Autor:Hammami P; Lancelot R; Domenech J; Lesnoff M
[Ad] Endereço:UMR 117 Animals, Health, Territories, Risks and Ecosystems (ASTRE), Centre de coopération internationale en recherche agronomique pour le développement (CIRAD), Campus international de Baillarguet, 34398 Montpellier, France.
[Ti] Título:Ex-ante assessment of different vaccination-based control schedules against the peste des petits ruminants virus in sub-Saharan Africa.
[So] Source:PLoS One;13(1):e0190296, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Peste des petits ruminants (PPR) is a highly contagious and widespread viral infection of small ruminants (goats and sheep), causing heavy economic losses in many developing countries. Therefore, its progressive control and global eradication by 2030 was defined as a priority by international organizations addressing animal health. The control phase of the global strategy is based on mass vaccination of small ruminant populations in endemic regions or countries. It is estimated that a 70% post-vaccination immunity rate (PVIR) is needed in a given epidemiological unit to prevent PPR virus spread. However, implementing mass vaccination is difficult and costly in smallholder farming systems with scattered livestock and limited facilities. Regarding this, controlling PPR is a special challenge in sub-Saharan Africa. In this study, we focused on this region to assess the effect of several variables of PVIR in two contrasted smallholder farming systems. METHODS: Using a seasonal matrix population model of PVIR, we estimated its decay in goats reared in sub-humid areas, and sheep reared in semi-arid areas, over a 4-year vaccination program. Assuming immunologically naive and PPR-free epidemiological unit, we assessed the ability of different vaccination scenarios to reach the 70% PVIR throughout the program. The tested scenarios differed in i) their overall schedule, ii) their delivery month and iii) their vaccination coverage. RESULTS: In sheep reared in semi-arid areas, the vaccination month did affect the PVIR decay though it did not in goats in humid regions. In both cases, our study highlighted i) the importance of targeting the whole eligible population at least during the two first years of the vaccination program and ii) the importance of reaching a vaccination coverage as high as 80% of this population. This study confirmed the relevance of the vaccination schedules recommended by international organizations.
[Mh] Termos MeSH primário: Doenças das Cabras/prevenção & controle
Esquemas de Imunização
Peste dos Pequenos Ruminantes/prevenção & controle
Vírus da Peste dos Pequenos Ruminantes/imunologia
Doenças dos Ovinos/prevenção & controle
Vacinas Virais/administração & dosagem
[Mh] Termos MeSH secundário: África ao Sul do Saara
Animais
Cabras
Ovinos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Viral Vaccines)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180215
[Lr] Data última revisão:
180215
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180120
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0190296


  6 / 4640 MEDLINE  
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[PMID]:29273058
[Au] Autor:Utaaker KS; Myhr N; Bajwa RS; Joshi H; Kumar A; Robertson LJ
[Ad] Endereço:Parasitology Laboratory, Department for Food Safety and Infection Biology, Faculty of Veterinary Medicine, Norwegian University of Life Sciences, Adamstuen Campus, PO Box 8146, Dep. 0033, Oslo, Norway. kjersti.selstad.utaaker@nmbu.no.
[Ti] Título:Goats in the city: prevalence of Giardia duodenalis and Cryptosporidium spp. in extensively reared goats in northern India.
[So] Source:Acta Vet Scand;59(1):86, 2017 Dec 22.
[Is] ISSN:1751-0147
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Various characteristics of goats mean they are highly suitable livestock for backyard rearing by people with limited resources. They are a popular livestock choice in India, where they are often kept to supplement an already scarce income. In these settings, hygiene and sanitation standards tend to be low, and weakens the interface between humans and animals, thus reducing the barrier between them and thereby increasing the likelihood that zoonotic and anthroponotic infections will occur. RESULTS: This study reports an investigation of the occurrence of Cryptosporidium spp. and Giardia duodenalis in goats being reared in different settings in urban and peri-urban areas in northern India, and addressed the zoonotic potential of these important protozoan parasites shed from goats living close to humans. The overall prevalence of G. duodenalis was 33.8 and 0.5% for Cryptosporidium spp.; the relatively low prevalence of cryptosporidiosis may reflect that most samples were derived from adult animals. The prevalence of G. duodenalis excretion was found to be similar to that reported in other studies. However, although other studies have reported a predominance of non-zoonotic Assemblage E in goats, in this study potentially zoonotic Assemblages predominated [Assemblage A (36%) and Assemblage B (32%)]. CONCLUSIONS: The results of this study indicate that in this area where goats and humans are living in close proximity, there may be sharing of intestinal parasites, which can be detrimental for both host species.
[Mh] Termos MeSH primário: Criptosporidiose/epidemiologia
Cryptosporidium/fisiologia
Giardia lamblia/fisiologia
Giardíase/veterinária
Doenças das Cabras/epidemiologia
[Mh] Termos MeSH secundário: Animais
Fezes/parasitologia
Giardia lamblia/classificação
Giardíase/epidemiologia
Giardíase/parasitologia
Doenças das Cabras/parasitologia
Cabras
Índia
Prevalência
População Urbana
Zoonoses/epidemiologia
Zoonoses/transmissão
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180214
[Lr] Data última revisão:
180214
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171224
[St] Status:MEDLINE
[do] DOI:10.1186/s13028-017-0354-4


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[PMID]:28459224
[Au] Autor:Hameed K; Riaz Ch F; Nawaz MA; Naqvi SMS; Gräser Y; Kupsch C; Pasquetti M; Rossi L; Molinar Min AR; Tizzani P; Chiavassa E; Peano A
[Ad] Endereço:Arid Agriculture University Rawalpindi, Rawalpindi, Pakistan. Khalid.zoology@must.edu.pk.
[Ti] Título:Trichophyton verrucosum infection in livestock in the Chitral district of Pakistan.
[So] Source:J Infect Dev Ctries;11(4):326-333, 2017 Apr 30.
[Is] ISSN:1972-2680
[Cp] País de publicação:Italy
[La] Idioma:eng
[Ab] Resumo:INTRODUCTION: Trichophyton verrucosum belongs to the dermatophyte fungi, closely related organisms that cause skin infections in animals and humans. T. verrucosum infection has been reported in livestock and people in different countries from all continents. Human cases have been reported in different areas of Pakistan, but there is little information about the animal source of the fungus. METHODOLOGY: Dermatological specimens collected in the Chitral district of Pakistan for a study on mange in livestock were retrospectively analyzed for the presence of T. verrucosum. In total, 5,873 animals (1,087 cows, 2,033 goats, and 2,753 sheep) were screened for evidence of dermatological lesions during two surveys performed in the summer and winter seasons. Skin scrapings collected from animals with lesions were analyzed by direct microscopic examination after digestion in sodium hydroxide and a real-time polymerase chain reaction (PCR) targeting pathogenic Trichophyton species. RESULTS: At microscopy, samples from 18 cows (1.6%), 3 sheep (0.1%), and 4 goats (0.2%) were positive for fungal elements consistent with T. verrucosum. PCR confirmed the microscopy results. The prevalence was lower than that reported in other countries in intensive breeding farms. Results agree with the literature regarding factors affecting T. verrucosum diffusion, i.e., infection was more prevalent in cattle, especially in younger animals during the winter season. CONCLUSIONS: This study reports, for the first time, the presence of T. verrucosum in livestock in Pakistan. A better knowledge of the animal role in the spread of this fungus may allow the adoption of more efficient control measures and prophylaxis.
[Mh] Termos MeSH primário: Doenças dos Bovinos/epidemiologia
Doenças das Cabras/epidemiologia
Doenças dos Ovinos/epidemiologia
Tinha/veterinária
Trichophyton/isolamento & purificação
[Mh] Termos MeSH secundário: Animais
Bovinos
Doenças dos Bovinos/microbiologia
Doenças das Cabras/microbiologia
Cabras
Microscopia
Paquistão/epidemiologia
Reação em Cadeia da Polimerase em Tempo Real
Estudos Retrospectivos
Ovinos
Doenças dos Ovinos/microbiologia
Tinha/epidemiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180109
[Lr] Data última revisão:
180109
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170502
[St] Status:MEDLINE
[do] DOI:10.3855/jidc.7925


  8 / 4640 MEDLINE  
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[PMID]:28449722
[Au] Autor:Bilgic HB; Bakirci S; Kose O; Unlu AH; Hacilarlioglu S; Eren H; Weir W; Karagenc T
[Ad] Endereço:Department of Parasitology, University of Adnan Menderes, Faculty of Veterinary Medicine, 09016, Isikli/Aydin, Turkey. hbilgic@adu.edu.tr.
[Ti] Título:Prevalence of tick-borne haemoparasites in small ruminants in Turkey and diagnostic sensitivity of single-PCR and RLB.
[So] Source:Parasit Vectors;10(1):211, 2017 Apr 27.
[Is] ISSN:1756-3305
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Tick-borne haemoparasitic diseases (TBHDs), caused by Theileria, Babesia, Anaplasma and Ehrlichia, are common in regions of the world where the distributions of host, pathogen and vector overlap. Many of these diseases threaten livestock production and some also represent a concern to human public health. The primary aim of this study was to determine the prevalence of the above-mentioned pathogens in a large number of blood samples (n = 1979) collected from sheep (n = 1727) and goats (n = 252) in Turkey. A secondary aim was to assess the diagnostic sensitivity of a number of species-specific polymerase chain reaction (PCR) tests and the reverse line blotting (RLB) assay. DNA samples were screened using species-specific PCR for the presence of Theileria ovis, Theileria sp. MK, T. lestoquardi, T. uilenbergi, T. luwenshuni, Babesia ovis, Anaplasma ovis and A. phagocytophilum while RLB was undertaken to test for the presence of all known Theileria, Babesia, Anaplasma and Ehrlichia species. The diagnostic sensitivity of these two approaches was then compared in terms of their ability to detect single species and mixed infections. RESULTS: Overall, 84 and 74.43% of the small ruminants sampled were identified as hosting one or more pathogen(s) by species-specific PCR and RLB respectively. The presence of Theileria sp. OT1, T. luwenshuni and T. uilenbergi in Turkey was revealed for the first time while the presence of Babesia motasi, B. crassa and T. separata in Turkish small ruminants was confirmed using molecular methods. A high prevalence of mixed infection was evident, with PCR and RLB approaches indicating that 52.24 and 35.42% of animals were co-infected with multiple species, respectively. More than 80% of the mixed infections contained T. ovis and/or A. ovis. The RLB approach was found to be capable of detecting mixed infections with species such as Theileria sp. OT1, Theileria sp. OT3, T. separata, B. crassa and Babesia spp. CONCLUSION: The results indicated that pathogens causing TBHDs are highly prevalent in sheep and goats in Turkey. The diagnostic sensitivity of species-specific single PCR was generally higher than that of RLB. However, the latter approach was still capable of identifying a high proportion of individuals containing mixed-species infections. The use of species-specific single PCR is recommended to accurately estimate pathogen prevalence and to identify co-infected hosts.
[Mh] Termos MeSH primário: Técnicas de Diagnóstico Molecular/métodos
Infecções Protozoárias em Animais/diagnóstico
Infecções Protozoárias em Animais/epidemiologia
Doenças Transmitidas por Carrapatos/veterinária
[Mh] Termos MeSH secundário: Animais
Doenças das Cabras/diagnóstico
Doenças das Cabras/epidemiologia
Cabras
Hibridização de Ácido Nucleico/métodos
Reação em Cadeia da Polimerase/métodos
Prevalência
Sensibilidade e Especificidade
Análise de Sequência de DNA
Ovinos
Doenças dos Ovinos/diagnóstico
Doenças dos Ovinos/epidemiologia
Doenças Transmitidas por Carrapatos/diagnóstico
Doenças Transmitidas por Carrapatos/epidemiologia
Carrapatos
Turquia/epidemiologia
[Pt] Tipo de publicação:COMPARATIVE STUDY; EVALUATION STUDIES; JOURNAL ARTICLE
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180109
[Lr] Data última revisão:
180109
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170429
[St] Status:MEDLINE
[do] DOI:10.1186/s13071-017-2151-3


  9 / 4640 MEDLINE  
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[PMID]:29284250
[Au] Autor:Filioussis G; Theodoridis A; Papadopoulos D; Gelasakis AI; Vouraki S; Bramis G; Arsenos G
[Ad] Endereço:Laboratory of Microbiology & Infectious Diseases, School of Veterinary Medicine, Faculty of Health Sciences, Aristotle University, Thessaloniki, Greece. georgefilious@vet.auth.gr.
[Ti] Título:Serological prevalence of Coxiella burnetii in dairy goats and ewes diagnosed with adverse pregnancy outcomes in Greece.
[So] Source:Ann Agric Environ Med;24(4):702-705, 2017 Dec 23.
[Is] ISSN:1898-2263
[Cp] País de publicação:Poland
[La] Idioma:eng
[Ab] Resumo:INTRODUCTION: Coxiella burnetii is an obligatory intracellular bacterial pathogen causing the zoonotic disease Q fever. The most common reservoirs of C. burnetii are wild mammals, birds and ticks. Pregnant domestic ruminants infected with this bacterium are also a major source of human infection. MATERIAL AND METHODS: The serological prevalence of C. burnetii in goats and sheep diagnosed with adverse pregnancy outcomes was assessed by undertaking a survey on 800 dairy goats and 800 dairy ewes reared in four different regions of Greece (Macedonia, Thrace, Thessaly, and Peloponnese). A stratified sampling was carried out, taking also as a criterion the age of the animals. Serum antibodies were analyzed by a commercial ELISA according to the manufacturer's recommendations. RESULTS: Generally, there was a statistically significantly higher serological prevalence of C. burnetii (14.4%) in goats compared to sheep (8%). Serological prevalence was higher in adults (15.5% in goats and 8.5% in sheep) compared to yearlings (7.4% in goats and 4.6% in sheep). The prevalence increased significantly with age only in goats. Finally, all animals reared in Peloponnese had a prevalence significantly higher (21% in goats and 18% in sheep) than animals reared in the other three regions. CONCLUSIONS: To the best of the authors' knowledge, this is the first report that associates C. burnetii with reproductive disturbances of domestic ruminants in Greece. However, considering the importance of coxiellosis for public health, further investigations are required on its epidemiology regarding abortion, premature delivery, stillbirth and weak offspring in small ruminants, as well as in other domestic and wild animal species.
[Mh] Termos MeSH primário: Coxiella burnetii/imunologia
Doenças das Cabras/diagnóstico
Complicações na Gravidez/veterinária
Febre Q/veterinária
Doenças dos Ovinos/diagnóstico
[Mh] Termos MeSH secundário: Animais
Anticorpos Antibacterianos/imunologia
Coxiella burnetii/isolamento & purificação
Ensaio de Imunoadsorção Enzimática/veterinária
Feminino
Doenças das Cabras/epidemiologia
Doenças das Cabras/imunologia
Doenças das Cabras/microbiologia
Cabras
Grécia/epidemiologia
Masculino
Gravidez
Complicações na Gravidez/diagnóstico
Complicações na Gravidez/imunologia
Complicações na Gravidez/microbiologia
Resultado da Gravidez
Prevalência
Febre Q/diagnóstico
Febre Q/epidemiologia
Febre Q/imunologia
Estudos Soroepidemiológicos
Ovinos
Doenças dos Ovinos/epidemiologia
Doenças dos Ovinos/imunologia
Doenças dos Ovinos/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Bacterial)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180105
[Lr] Data última revisão:
180105
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171230
[St] Status:MEDLINE


  10 / 4640 MEDLINE  
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[PMID]:28865204
[Au] Autor:Panneum S; Rukkwamsuk T
[Ad] Endereço:.
[Ti] Título:Diagnosis of Caprine Arthritis Encephalitis Virus infection in dairy goats by ELISA, PCR and Viral Culture.
[So] Source:Pol J Vet Sci;20(2):347-353, 2017 Mar 01.
[Is] ISSN:1505-1773
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:For preventive and control strategies of Caprine Arthritis Encephalitis Virus (CAEV) infection in dairy goats, performance of the available diagnostic tests was described as one of the most important and necessary aspects. The study aimed at evaluating the diagnostic test performance, including PCR, ELISA and viral culture, for CAEV infection in dairy goats in Thailand. Blood samples of 29 dairy goats from five low- to medium-prevalence herds and one very low-prevalence herd were collected for PCR and ELISA methods. The performance of these two diagnostic methods was evaluated by comparing with cytopathic effects (CPE) in the co-cultivation of CAEV and primary synovial cells. Results indicated that sensitivity, specificity were, respectively, 69.6%, 100%, for PCR; and 95.7%, 83.3% for ELISA. The PCR assay tended to have lower sensitivity and higher specificity than ELISA. When multiple tests were applied, parallel testing provided sensitivity and specificity of 98.7% and 83.3%, while series testing showed sensitivity and specificity of 66.6% and 100% respectively. These results indicated that combination of ELISA and PCR provided some advantages and possibly offered optimal methods to detect CAEV-infected goats. Kappa value of the agreement between PCR and ELISA test was 0.34, indicating fair agreement. Regarding the possibility of antigenic variation between CAEV strains used in both PCR and ELISA assays, the actual circulating CAEV strain should be reviewed in order to develop and enhance the diagnostic tests using the CAE viral antigens derived from specific local strains of Thailand.
[Mh] Termos MeSH primário: Vírus da Artrite-Encefalite Caprina
Ensaio de Imunoadsorção Enzimática/veterinária
Doenças das Cabras/virologia
Infecções por Lentivirus/veterinária
Reação em Cadeia da Polimerase/veterinária
Cultura de Vírus/veterinária
[Mh] Termos MeSH secundário: Animais
Feminino
Doenças das Cabras/diagnóstico
Cabras
Infecções por Lentivirus/diagnóstico
Infecções por Lentivirus/virologia
Reação em Cadeia da Polimerase/métodos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171106
[Lr] Data última revisão:
171106
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170903
[St] Status:MEDLINE



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