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[PMID]:29349655
[Au] Autor:Lo WJ; Lin CL; Chang YC; Bai LY; Lin CY; Liang JA; Li LY; Chao LM; Chiu CF; Chen CM; Yeh SP
[Ad] Endereço:Department of Life Sciences, National Chung Hsing University, Taichung, Taiwan.
[Ti] Título:Total body irradiation tremendously impair the proliferation, differentiation and chromosomal integrity of bone marrow-derived mesenchymal stromal stem cells.
[So] Source:Ann Hematol;97(4):697-707, 2018 Apr.
[Is] ISSN:1432-0584
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Total body irradiation (TBI) is frequently used in hematopoietic stem cell transplantation (HSCT) and is associated with many complications due to radiation injury to the normal cells, including normal stem cells. Nevertheless, the effects of TBI on the mesenchymal stromal stem cell (MSC) are not fully understood. Bone marrow-derived MSCs (BM-MSCs) isolated from normal adults were irradiated with 200 cGy twice daily for consecutive 3 days, a regimen identical to that used in TBI-conditioning HSCT. The characteristics, differentiation potential, cytogenetics, hematopoiesis-supporting function, and carcinogenicity of the irradiated BM-MSCs were then compared to the non-irradiated control. The irradiated and non-irradiated MSCs shared similar morphology, phenotype, and hematopoiesis-supporting function. However, irradiated MSCs showed much lower proliferative and differentiative potential. Irradiation also induced clonal cytogenetic abnormalities of MSCs. Nevertheless, the carcinogenicity of irradiated MSCs is low in vitro and in vivo. In parallel with the ex vivo irradiation experiments, decreased proliferative and differentiative abilities and clonal cytogenetic abnormalities can also be found in MSCs isolated from transplant recipients who had received TBI-based conditioning previously. Thus, TBI used in HSCT drastically injury MSCs and may contribute to the development of some long-term complications associated with clonal cytogenetic abnormality and poor adipogenesis and osteogenesis after TBI.
[Mh] Termos MeSH primário: Apoptose/efeitos da radiação
Células da Medula Óssea/efeitos da radiação
Aberrações Cromossômicas/efeitos da radiação
Células-Tronco Hematopoéticas/efeitos da radiação
Células Mesenquimais Estromais/efeitos da radiação
Lesões por Radiação/patologia
Irradiação Corporal Total/efeitos adversos
[Mh] Termos MeSH secundário: Adulto
Células-Tronco Adultas/efeitos da radiação
Células da Medula Óssea/citologia
Células da Medula Óssea/patologia
Diferenciação Celular/efeitos da radiação
Proliferação Celular/efeitos da radiação
Células Cultivadas
China
Transtornos Cromossômicos/etiologia
Transtornos Cromossômicos/patologia
Feminino
Transplante de Células-Tronco Hematopoéticas
Células-Tronco Hematopoéticas/citologia
Células-Tronco Hematopoéticas/patologia
Hospitais Universitários
Seres Humanos
Leucemia/patologia
Leucemia/terapia
Masculino
Células Mesenquimais Estromais/citologia
Células Mesenquimais Estromais/patologia
Necrose
Lesões por Radiação/etiologia
Condicionamento Pré-Transplante/efeitos adversos
Células Tumorais Cultivadas
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180120
[St] Status:MEDLINE
[do] DOI:10.1007/s00277-018-3231-y


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[PMID]:29205972
[Au] Autor:Su Q; Bu F; Chen C; Shi Y; Lu ZY; Liu YC
[Ad] Endereço:Forensic Science Service of Beijing Public Security Bureau, Beijing 100192, China.
[Ti] Título:[Microdeletion and Mutation of Y Chromosome in Full Sibling Identification].
[So] Source:Fa Yi Xue Za Zhi;32(6):438-440, 2016 Dec.
[Is] ISSN:1004-5619
[Cp] País de publicação:China
[La] Idioma:chi
[Ab] Resumo:OBJECTIVES: To explore the identification method of full sibling between two males with microdeletion and mutation of Y chromosome. METHODS: DNA were extracted from two samples. The type testing of Y-STR and autosomal STR were performed. Full sibling between two individuals was calculated by IBS, ITO and discriminant functions methods. RESULTS: There were 2 loci mutations existed in 33 Y-STR loci and one of the two samples had 19 loci deletions. The IBS of two samples was 53 and greater than the threshold which was 42; FSI was 1.36×10¹6 and far greater than 19. The discriminant function of full sibling-unrelated individual was greater than , which meant the two individuals tend to be full sibling. CONCLUSIONS: The methods of IBS, ITO and discriminant functions of full sibling-unrelated individual can be used comprehensively to provide more reliable expert opinion in microdeletion and mutation of Y chromosome in full sibling identification.
[Mh] Termos MeSH primário: Aberrações Cromossômicas
Cromossomos Humanos Y/genética
Genética Forense
Deleção de Sequência
[Mh] Termos MeSH secundário: Alelos
Análise Discriminante
Seres Humanos
Masculino
Reação em Cadeia da Polimerase
Irmãos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180308
[Lr] Data última revisão:
180308
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171206
[St] Status:MEDLINE
[do] DOI:10.3969/j.issn.1004-5619.2016.06.011


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[PMID]:29351567
[Au] Autor:Ghandhi SA; Turner HC; Shuryak I; Dugan GO; Bourland JD; Olson JD; Tooze JA; Morton SR; Batinic-Haberle I; Cline JM; Amundson SA
[Ad] Endereço:Center for Radiological Research, Columbia University Medical Center, New York, New York, United States of America.
[Ti] Título:Whole thorax irradiation of non-human primates induces persistent nuclear damage and gene expression changes in peripheral blood cells.
[So] Source:PLoS One;13(1):e0191402, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:We investigated the cytogenetic and gene expression responses of peripheral blood cells of non-human primates (NHP, Macaca mulatta) that were whole-thorax irradiated with a single dose of 10 Gy. In this model, partial irradiation of NHPs in the thoracic region (Whole Thorax Lung Irradiation, WTLI) allows the study of late radiation-induced lung injury, while avoiding acute radiation syndromes related to hematopoietic and gastrointestinal injury. A transient drop in circulating lymphocytes and platelets was seen by 9 days, followed by elevations in respiratory rate, circulating neutrophils, lymphocytes, and monocytes at 60-100 days, corresponding to computed tomography (CT) and histologic evidence of pneumonitis, and elective euthanasia of four animals. To evaluate long-term DNA damage in NHP peripheral blood lymphocytes after 10 Gy WTLI, we used the cytokinesis-block micronucleus (CBMN) assay to measure chromosomal aberrations as post-mitotic micronuclei in blood samples collected up to 8 months after irradiation. Regression analysis showed significant induction of micronuclei in NHP blood cells that persisted with a gradual decline over the 8-month study period, suggesting long-term DNA damage in blood lymphocytes after WTLI. We also report transcriptomic changes in blood up to 30 days after WTLI. We isolated total RNA from peripheral blood at 3 days before and then at 2, 5 and 30 days after irradiation. We identified 1187 transcripts that were significantly changed across the 30-day time course. From changes in gene expression, we identified biological processes related to immune responses, which persisted across the 30-day study. Response to oxygen-containing compounds and bacteria were implicated by gene-expression changes at the earliest day 2 and latest, day 30 time-points. Gene expression changes suggest a persistent altered state of the immune system, specifically response to infection, for at least a month after WTLI.
[Mh] Termos MeSH primário: Células Sanguíneas/metabolismo
Células Sanguíneas/efeitos da radiação
Dano ao DNA
Expressão Gênica/efeitos da radiação
[Mh] Termos MeSH secundário: Animais
Contagem de Células Sanguíneas
Aberrações Cromossômicas
Relação Dose-Resposta à Radiação
Ontologia Genética
Seres Humanos
Lesão Pulmonar/sangue
Lesão Pulmonar/etiologia
Lesão Pulmonar/genética
Macaca mulatta/sangue
Macaca mulatta/genética
Masculino
Testes para Micronúcleos
Lesões Experimentais por Radiação/sangue
Lesões Experimentais por Radiação/genética
Tórax/efeitos da radiação
Fatores de Tempo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180305
[Lr] Data última revisão:
180305
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180120
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0191402


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[PMID]:28460439
[Au] Autor:Pant V; Larsson CA; Aryal N; Xiong S; You MJ; Quintas-Cardama A; Lozano G
[Ad] Endereço:Department of Genetics, M.D. Anderson Cancer Center, Houston, Texas, 77030, USA.
[Ti] Título:Tumorigenesis promotes Mdm4-S overexpression.
[So] Source:Oncotarget;8(16):25837-25847, 2017 Apr 18.
[Is] ISSN:1949-2553
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Disruption of the p53 tumor suppressor pathway is a primary cause of tumorigenesis. In addition to mutation of the p53 gene itself, overexpression of major negative regulators of p53, MDM2 and MDM4, also act as drivers for tumor development. Recent studies suggest that expression of splice variants of Mdm2 and Mdm4 may be similarly involved in tumor development. In particular, multiple studies show that expression of a splice variant of MDM4, MDM4-S correlates with tumor aggressiveness and can be used as a prognostic marker in different tumor types. However, in the absence of prospective studies, it is not clear whether expression of MDM4-S in itself is oncogenic or is simply an outcome of tumorigenesis. Here we have examined the role of Mdm4-S in tumor development in a transgenic mouse model. Our results suggest that splicing of Mdm4 does not promote tumor development and does not cooperate with other oncogenic insults to alter tumor latency or aggressiveness. We conclude that Mdm4-S overexpression is a consequence of splicing defects in tumor cells rather than a cause of tumor evolution.
[Mh] Termos MeSH primário: Transformação Celular Neoplásica/genética
Expressão Gênica
Proteínas Nucleares/genética
Proteínas Proto-Oncogênicas/genética
[Mh] Termos MeSH secundário: Idoso
Animais
Biomarcadores
Linhagem Celular Tumoral
Aberrações Cromossômicas
Modelos Animais de Doenças
Feminino
Regulação Neoplásica da Expressão Gênica
Seres Humanos
Leucemia Mielogênica Crônica BCR-ABL Positiva/genética
Leucemia Mielogênica Crônica BCR-ABL Positiva/mortalidade
Leucemia Mielogênica Crônica BCR-ABL Positiva/patologia
Masculino
Camundongos
Camundongos Transgênicos
Meia-Idade
Mutação
Polimorfismo de Nucleotídeo Único
Processamento de RNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biomarkers); 0 (MDM4 protein, human); 0 (Nuclear Proteins); 0 (Proto-Oncogene Proteins)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180305
[Lr] Data última revisão:
180305
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170503
[St] Status:MEDLINE
[do] DOI:10.18632/oncotarget.15552


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[PMID]:29391062
[Au] Autor:Lee MY; Park YC; Jin M; Kim E; Choi JJ; Jung IC
[Ad] Endereço:Genogen Co., Ltd, Room 402, 125, Osongsaengmyeong 2-ro, Osong-eup, Heungdeok-gu, Cheongju-si, Chungcheongbuk-do, 28161, Republic of Korea.
[Ti] Título:Genotoxicity evaluation of So-ochim-tang-gamibang (SOCG), a herbal medicine.
[So] Source:BMC Complement Altern Med;18(1):47, 2018 Feb 02.
[Is] ISSN:1472-6882
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: So-ochim-tang-gamibang (SOCG) is a traditional Korean medicine frequently used for depression in the clinical field. In this study, we evaluated the potential genotoxicity of SOCG using three standard batteries of tests as part of a safety evaluation. METHODS: SOCG was evaluated for potential genotoxic effects using the standard three tests recommended by the Ministry of Food and Drug Safety (MFDS) of Korea. These tests were the bacterial reverse mutation test (Ames test), in vitro mammalian chromosomal aberration test using Chinese hamster lung cells, and in vivo micronucleus test using ICR mice. RESULTS: The Ames test with Salmonella typhimurium strains TA98, TA100, TA1535 and TA1537 and the Escherichia coli strain WP2uvrA(pKM101) showed that SOCG did not induce gene mutations at any dose level in all of the strains. SOCG did not induce any chromosomal aberrations in the in vitro chromosomal aberration test (for both the 6 and 24 h test) and the in vivo micronucleus test. CONCLUSIONS: Based on the results of these tests, it was concluded that SOCG does not exhibit any genotoxic risk under the experimental conditions of this study.
[Mh] Termos MeSH primário: Mutagênicos/toxicidade
Extratos Vegetais/toxicidade
[Mh] Termos MeSH secundário: Animais
Linhagem Celular
Aberrações Cromossômicas/efeitos dos fármacos
Cricetinae
Cricetulus
Escherichia coli/efeitos dos fármacos
Masculino
Medicina Tradicional Coreana
Camundongos
Camundongos Endogâmicos ICR
Micronúcleos com Defeito Cromossômico/efeitos dos fármacos
Testes para Micronúcleos
Testes de Mutagenicidade
Salmonella typhimurium/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Mutagens); 0 (Plant Extracts); 0 (so-ochim-tang-gamibang)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180301
[Lr] Data última revisão:
180301
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180203
[St] Status:MEDLINE
[do] DOI:10.1186/s12906-018-2111-2


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[PMID]:28457629
[Au] Autor:McCoy RC
[Ad] Endereço:Department of Genome Sciences, University of Washington, Seattle, WA, USA. Electronic address: rcmccoy@uw.edu.
[Ti] Título:Mosaicism in Preimplantation Human Embryos: When Chromosomal Abnormalities Are the Norm.
[So] Source:Trends Genet;33(7):448-463, 2017 07.
[Is] ISSN:0168-9525
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Along with errors in meiosis, mitotic errors during post-zygotic cell division contribute to pervasive aneuploidy in human embryos. Relatively little is known, however, about the genesis of these errors or their fitness consequences. Rapid technological advances are helping to close this gap, revealing diverse molecular mechanisms contributing to mitotic error. These include altered cell cycle checkpoints, aberrations of the centrosome, and failed chromatid cohesion, mirroring findings from cancer biology. Recent studies are challenging the idea that mitotic error is abnormal, emphasizing that the fitness impacts of mosaicism depend on its scope and severity. In light of these findings, technical and philosophical limitations of various screening approaches are discussed, along with avenues for future research.
[Mh] Termos MeSH primário: Blastocisto
Aberrações Cromossômicas
Mosaicismo
[Mh] Termos MeSH secundário: Aneuploidia
Seres Humanos
Meiose
Mitose
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW; RESEARCH SUPPORT, N.I.H., EXTRAMURAL
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180228
[Lr] Data última revisão:
180228
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170502
[St] Status:MEDLINE


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[PMID]:29192617
[Au] Autor:Vasilyeva IN; Bespalov VG; Semenov AL; Baranenko DA; Zinkin VN
[Ad] Endereço:Scientific Laboratory for Cancer Chemoprevention and Oncopharmacology at N.N. Petrov Research Institute of Oncology under the Ministry of Health of the Russian Federation, Moscow; International Research Centre "Biotechnologies of the Third Millennium", ITMO University, St. Petersburg, Russian Federa
[Ti] Título:The Effects of Low-Frequency Noise on Rats: Evidence of Chromosomal Aberrations in the Bone Marrow Cells and the Release of Low-Molecular-Weight DNA in the Blood Plasma.
[So] Source:Noise Health;19(87):79-83, 2017 Mar-Apr.
[Is] ISSN:1463-1741
[Cp] País de publicação:India
[La] Idioma:eng
[Ab] Resumo:OBJECTIVES: Evaluation of the effect of low-frequency noise (LFN) on the frequency of chromosomal aberrations in the bone marrow cells and on the content of low-molecular-weight DNA (lmwDNA) in the blood plasma of rats. MATERIALS AND METHODS: A total of 96 male Wistar rats were exposed to either single (17 min session) or multiple (17 min session repeated five times a week for 13 weeks) LFN, with the maximum range below 250 Hz and the sound pressure levels (SPLs) at 120 and 150 dB, respectively. The rats in the control groups were not subjected to any impact. The frequency of chromosomal aberrations in the bone marrow cells and the levels of lmwDNA in the blood plasma were measured afterwards. RESULTS: It has been detected that a single LFN exposure with either corresponding SPLs had a significant increase in the frequency of chromosomal aberrations (more than 10-fold) compared to the controls (0.9 ± 0.3%) and resulted in the appearance of dicentric chromosomes in the aberration spectrum, both of which are evident for the occurrence of deoxyribonucleic acid double strand breaks triggered by the exposure. Furthermore, the lmwDNA levels in the blood plasma measured the following day after a single LFN exposure were significantly higher (7.7- and 7.6-fold, respectively) than that in the control group (11.0 ± 5.4 ng/ml), and such levels were maintained higher (4.8- and 2.1-fold, respectively) in the week after a single LFN exposure for the SPL of 120 and 150 dB, respectively, compared to the control group (18.8 ± 1.6 ng/ml). Similar results were obtained from the group with multiple LFN exposures (36.4- and 22.4-fold, respectively) compared to the control (17.7 ± 1.7 ng/ml) and suggest the enhancement of cellular apoptosis as a result of the LFN impact. CONCLUSION: Presumably, the LFN may have possible mutagenic effects and cause massive cell death.
[Mh] Termos MeSH primário: Células da Medula Óssea/patologia
Aberrações Cromossômicas
DNA/sangue
Ruído/efeitos adversos
[Mh] Termos MeSH secundário: Animais
Masculino
Peso Molecular
Ratos
Ratos Wistar
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
9007-49-2 (DNA)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180227
[Lr] Data última revisão:
180227
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171202
[St] Status:MEDLINE
[do] DOI:10.4103/nah.NAH_39_16


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[PMID]:29265181
[Au] Autor:Horai M; Satoh S; Matsuo M; Iwanaga M; Horio K; Jo T; Takasaki Y; Kawaguchi Y; Tsushima H; Yoshida S; Taguchi M; Itonaga H; Sawayama Y; Taguchi J; Imaizumi Y; Hata T; Moriuchi Y; Haase D; Yoshiura KI; Miyazaki Y
[Ad] Endereço:Department of Haematology, Atomic Bomb Disease and Hibakusha Medicine Unit, Atomic Bomb Disease Institute, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan.
[Ti] Título:Chromosomal analysis of myelodysplastic syndromes among atomic bomb survivors in Nagasaki.
[So] Source:Br J Haematol;180(3):381-390, 2018 02.
[Is] ISSN:1365-2141
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The myelodysplastic syndromes (MDS) are clonal haematopoietic disorders that develop de novo and also secondary to chemotherapy and/or radiation therapy. We previously demonstrated that the risk of MDS is increased among atomic bomb survivors with significant correlation to radiation dose; however, the clinical characteristics of these survivors have not been well analysed. In this study, we investigated chromosomal abnormalities of MDS among survivors. The frequency of abnormal karyotypes was significantly higher, with more very poor risk karyotypes, according to the revised International Prognostic Scoring System, among those exposed close to the hypocentre compared with unexposed cases. However, abnormal karyotype frequency did not reflect the prognosis of exposed cases with respect to distance from the hypocentre. In addition, there was no difference in prognosis between exposed and unexposed cases. Among proximally exposed cases (<1·5 km from the hypocentre), chromosomal translocations and inversions were more frequent, and the frequency of structural alterations in chromosomes 3, 8, and 11 was significantly increased compared with unexposed cases. These results suggest that chromosomal alterations in MDS among survivors have different features compared with those in de novo or therapy-related MDS. Detailed molecular study is warranted.
[Mh] Termos MeSH primário: Aberrações Cromossômicas
Vítimas de Desastres
Síndromes Mielodisplásicas/epidemiologia
Síndromes Mielodisplásicas/genética
Armas Nucleares
Sobreviventes
[Mh] Termos MeSH secundário: Adulto
Idoso
Idoso de 80 Anos ou mais
Contagem de Células Sanguíneas
Medula Óssea/patologia
Análise Citogenética
Feminino
Seres Humanos
Japão/epidemiologia
Masculino
Meia-Idade
Síndromes Mielodisplásicas/diagnóstico
Síndromes Mielodisplásicas/terapia
Avaliação de Resultados da Assistência ao Paciente
Sistema de Registros
Análise de Sobrevida
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180222
[Lr] Data última revisão:
180222
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171222
[St] Status:MEDLINE
[do] DOI:10.1111/bjh.15050


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[PMID]:29357857
[Au] Autor:Lee MY; Seo CS; Ha H; Park E; Kim JY; Shin HK
[Ad] Endereço:K-herb Research Center, Korea Institute of Oriental Medicine, 1672 Yuseongdae-ro, Yuseong-gu, Daejeon, 305-811, Republic of Korea.
[Ti] Título:The genotoxicity of an aqueous extract of Gyejibokryeong-hwan.
[So] Source:BMC Complement Altern Med;18(1):21, 2018 Jan 22.
[Is] ISSN:1472-6882
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Gyejibokryeong-hwan (Guizhi Fuling Wan in China), a mixture of five herbal plants, is a well-known treatment for renal diseases including those associated with climacteric syndrome. However, the genotoxicity of Gyejibokryeong-hwan has not yet been well established. METHODS: The present study investigated that the genotoxicity of an aqueous extract of Gyejibokryeong-hwan (GJBRHE): an in vitro chromosomal aberration test using Chinese hamster lung cells, an in vitro bacterial reverse mutation assay (Ames test) with Salmonella typhimurium and Escherichia coli strains, and an in vivo micronucleus test using ICR mouse bone marrow. RESULTS: GJBRHE with or without the S9 mix showed no genotoxicity in the Ames test up to 5000 µg/plate or in the in vivo MN test up to 2000 mg/kg body weight. In contrast, the chromosomal aberration test showed that GJBRHE induced an increase in the number of chromosomal aberrations compared with the control after treatment for 6 h with 4200 µg/mL GJBRHE in the presence of the S9 mix and for 22 h with 800 µg/mL GJBRHE in the absence of the S9 mix. CONCLUSIONS: GJBRHE did not cause detectable genotoxic effects in the bacterial mutation test or the in vivo MN test, however genotoxic effect was detected in the in vitro chromosomal aberration assay. Our results suggest that GJBRHE may be associated with a low risk of carcinogenesis. Thus, further detailed experiments would be needed to clarify the compound responsible for inducing this genotoxicity of GJBRHE and to determine its mechanism.
[Mh] Termos MeSH primário: Aberrações Cromossômicas/efeitos dos fármacos
Dano ao DNA/efeitos dos fármacos
Medicamentos de Ervas Chinesas/toxicidade
Mutagênicos/toxicidade
[Mh] Termos MeSH secundário: Animais
Peso Corporal/efeitos dos fármacos
Medula Óssea/efeitos dos fármacos
Linhagem Celular
Cricetinae
Escherichia coli/efeitos dos fármacos
Camundongos
Testes para Micronúcleos
Testes de Mutagenicidade
Salmonella typhimurium/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Drugs, Chinese Herbal); 0 (Mutagens); 0 (keishibukuryogan)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180216
[Lr] Data última revisão:
180216
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180124
[St] Status:MEDLINE
[do] DOI:10.1186/s12906-017-2054-z


  10 / 58046 MEDLINE  
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[PMID]:29205009
[Au] Autor:Huang JP; Yang F; Liu YN; Zou KN; Cao Y; Wu D; Chen RH; Ping Y; Zhou HG
[Ad] Endereço:Shanghai Key Laboratory of Crime Scene Evidence, Key Laboratory of Forensic Evidence and Science Technology, Ministry of Public Security, Institute of Forensic Science, Shanghai Public Security Bureau, Shanghai 200083, China.
[Ti] Título:[Research Progress on Gene Alterations of Locus in Gender Identification].
[So] Source:Fa Yi Xue Za Zhi;32(5):371-377, 2016 Oct.
[Is] ISSN:1004-5619
[Cp] País de publicação:China
[La] Idioma:chi
[Ab] Resumo:There are two kinds of gene mutation, including mutation in primer-binding region of gene and micro deletion of Y chromosome encompassing gene, and the latter is more common. The mechanisms of mutation in primer-binding region of gene is nucleotide point mutation and the mechanism of micro deletion of Y chromosome encompassing gene maybe non-allelic homologous recombination or non-homologous end-joining. Among the population worldwide, there is a notably higher frequency of gene mutations in Indian population, Sri Lanka population and Nepalese population which reside within the Indian subcontinent. Though gene mutations have little impact on fertility and phenotype, they might cause incorrect result in gender identification. Using composite-amplification kit which including autosomal STR locus, gene locus and multiple Y-STR locus, could avoid wrong gender identification caused by gene mutation.
[Mh] Termos MeSH primário: Amelogenina/genética
Aberrações Cromossômicas
Cromossomos Humanos Y/genética
[Mh] Termos MeSH secundário: Alelos
Grupo com Ancestrais do Continente Asiático/genética
Seres Humanos
Índia
Masculino
Repetições de Microssatélites
Nepal
Reação em Cadeia da Polimerase
Deleção de Sequência
Sri Lanka
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Amelogenin)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180215
[Lr] Data última revisão:
180215
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171206
[St] Status:MEDLINE
[do] DOI:10.3969/j.issn.1004-5619.2016.05.013



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