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[PMID]:29329320
[Au] Autor:Matondo RB; Moreno E; Toussaint MJM; Tooten PCJ; van Essen SC; van Liere EA; Youssef SA; Bongiovanni L; de Bruin A
[Ad] Endereço:Department of Pathobiology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands.
[Ti] Título:Atypical E2f functions are critical for pancreas polyploidization.
[So] Source:PLoS One;13(1):e0190899, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The presence of polyploid cells in the endocrine and exocrine pancreas has been reported for four decades. In rodents, pancreatic polyploidization is initiated after weaning and the number of polyploid cells increases with age. Surprisingly the molecular regulators and biological functions of polyploidization in the pancreas are still unknown. We discovered that atypical E2f activity is essential for polyploidization in the pancreas, using an inducible Cre/LoxP approach in new-born mice to delete ubiquitously the atypical E2f transcription factors, E2f7 and E2f8. In contrast to its critical role in embryonic survival, conditional deletion of both of both atypical E2fs in newborn mice had no impact on postnatal survival and mice lived until old age. However, deficiency of E2f7 or E2f8 alone was sufficient to suppress polyploidization in the pancreas and associated with only a minor decrease in blood serum levels of glucose, insulin, amylase and lipase under 4 hours starvation condition compared to wildtype littermates. In mice with fewer pancreatic polyploid cells that were fed ad libitum, no major impact on hormones or enzymes levels was observed. In summary, we identified atypical E2fs to be essential for polyploidization in the pancreas and discovered that postnatal induced loss of both atypical E2fs in many organs is compatible with life until old age.
[Mh] Termos MeSH primário: Fatores de Transcrição E2F/fisiologia
Pâncreas/citologia
Poliploidia
[Mh] Termos MeSH secundário: Amilases/sangue
Animais
Glicemia/metabolismo
Crescimento
Insulina/sangue
Lipase/sangue
Camundongos
Análise de Sobrevida
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Blood Glucose); 0 (E2F Transcription Factors); 0 (Insulin); EC 3.1.1.3 (Lipase); EC 3.2.1.- (Amylases)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180215
[Lr] Data última revisão:
180215
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180113
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0190899


  2 / 7470 MEDLINE  
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[PMID]:29293495
[Au] Autor:Rimbert H; Darrier B; Navarro J; Kitt J; Choulet F; Leveugle M; Duarte J; Rivière N; Eversole K; Le Gouis J; Davassi A; Balfourier F; Le Paslier MC; Berard A; Brunel D; Feuillet C; Poncet C; Sourdille P; Paux E; International Wheat Genome Sequencing Consortium; on behalf The BreedWheat Consortium
[Ad] Endereço:GDEC, INRA, Université Clermont Auvergne, Clermont-Ferrand, France.
[Ti] Título:High throughput SNP discovery and genotyping in hexaploid wheat.
[So] Source:PLoS One;13(1):e0186329, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Because of their abundance and their amenability to high-throughput genotyping techniques, Single Nucleotide Polymorphisms (SNPs) are powerful tools for efficient genetics and genomics studies, including characterization of genetic resources, genome-wide association studies and genomic selection. In wheat, most of the previous SNP discovery initiatives targeted the coding fraction, leaving almost 98% of the wheat genome largely unexploited. Here we report on the use of whole-genome resequencing data from eight wheat lines to mine for SNPs in the genic, the repetitive and non-repetitive intergenic fractions of the wheat genome. Eventually, we identified 3.3 million SNPs, 49% being located on the B-genome, 41% on the A-genome and 10% on the D-genome. We also describe the development of the TaBW280K high-throughput genotyping array containing 280,226 SNPs. Performance of this chip was examined by genotyping a set of 96 wheat accessions representing the worldwide diversity. Sixty-nine percent of the SNPs can be efficiently scored, half of them showing a diploid-like clustering. The TaBW280K was proven to be a very efficient tool for diversity analyses, as well as for breeding as it can discriminate between closely related elite varieties. Finally, the TaBW280K array was used to genotype a population derived from a cross between Chinese Spring and Renan, leading to the construction a dense genetic map comprising 83,721 markers. The results described here will provide the wheat community with powerful tools for both basic and applied research.
[Mh] Termos MeSH primário: Genótipo
Polimorfismo de Nucleotídeo Único
Poliploidia
Triticum/genética
[Mh] Termos MeSH secundário: Genes de Plantas
Filogenia
Triticum/classificação
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180215
[Lr] Data última revisão:
180215
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180103
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0186329


  3 / 7470 MEDLINE  
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[PMID]:29020092
[Au] Autor:Kim JH; Chung IK; Kim KM
[Ad] Endereço:School of Applied Biosciences, College of Agriculture & Life Sciences, Kyungpook National University, Daegu, Korea.
[Ti] Título:Construction of a genetic map using EST-SSR markers and QTL analysis of major agronomic characters in hexaploid sweet potato (Ipomoea batatas (L.) Lam).
[So] Source:PLoS One;12(10):e0185073, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The Sweet potato, Ipomoea batatas (L.) Lam, is difficult to study in genetics and genomics because it is a hexaploid. The sweet potato study not have been performed domestically or internationally. In this study was performed to construct genetic map and quantitative trait loci (QTL) analysis. A total of 245 EST-SSR markers were developed, and the map was constructed by using 210 of those markers. The total map length was 1508.1 cM, and the mean distance between markers was 7.2 cM. Fifteen characteristics were investigated for QTLs analysis. According to those, the Four QTLs were identified, and The LOD score was 3.0. Further studies need to develop molecular markers in terms of EST-SSR markers for doing to be capable of efficient breeding. The genetic map created here using EST-SSR markers will facilitate planned breeding of sweet potato cultivars with various desirable traits.
[Mh] Termos MeSH primário: Agricultura
Mapeamento Cromossômico/métodos
Etiquetas de Sequências Expressas
Ipomoea batatas/genética
Repetições de Microssatélites/genética
Poliploidia
Locos de Características Quantitativas/genética
[Mh] Termos MeSH secundário: Marcadores Genéticos
Característica Quantitativa Herdável
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Genetic Markers)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
171031
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171012
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0185073


  4 / 7470 MEDLINE  
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[PMID]:28953941
[Au] Autor:Liu Y; Chen Y; Gong Q; Lai J; Du J; Deng X
[Ad] Endereço:The Fishery Institute of the Sichuan Academy of Agricultural Sciences, Chengdu, China.
[Ti] Título:Paternity assignment in the polyploid Acipenser dabryanus based on a novel microsatellite marker system.
[So] Source:PLoS One;12(9):e0185280, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Acipenser dabryanus is listed as a Critical Endangered species in the IUCN Red List and the first class protected animals in China. Fortunately, A. dabryanus specimens are being successfully bred in captivity for conservation. However, for effective ex situ conservation, we should be aware of the genetic diversity and the degree of relatedness of the individuals selected for breeding. In this study, we aimed at the development of novel and reliable microsatellites used for the genetic study of A. dabryanus. A total of 14,321 simple sequence repeats (SSRs) were detected by transcriptome sequencing and screening. We selected 20 novel and polymorphic microsatellites (non-dinucleotide) with good repeatability from the 100 tested loci for a subsequent genetic and paternity study. A set of captive broodstock (F1 stock, n = 43) and their offspring (F2 stock, n = 96) were used to examine the efficiency of the 20 SSRs for assigning parentage to offspring, with an allocation success of 91.7%. We also found that only a few families predominantly contributed to the progeny produced by the 43 breeders. In addition, mitochondrial DNA data showed that the captive broodstock (F1 individuals) had an excellent probability of the same lineage, implying that a high level of inbreeding may have occurred in these individuals. Our research provides useful information on genetic diversity and reproductive pattern of A. dabryanus, and the 20 SSRs developed in this study can be applied to the future breeding program to avoid inbreeding for this stock or other related species of Acipenseriformes.
[Mh] Termos MeSH primário: Peixes/genética
Repetições de Microssatélites/genética
Herança Paterna/genética
Poliploidia
[Mh] Termos MeSH secundário: Animais
Sequência de Bases
DNA Mitocondrial/genética
Feminino
Variação Genética
Masculino
Motivos de Nucleotídeos/genética
Reprodução/genética
Transcriptoma/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Mitochondrial)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171016
[Lr] Data última revisão:
171016
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170928
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0185280


  5 / 7470 MEDLINE  
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[PMID]:28950101
[Au] Autor:Cao J; Wang J; Jackman CP; Cox AH; Trembley MA; Balowski JJ; Cox BD; De Simone A; Dickson AL; Di Talia S; Small EM; Kiehart DP; Bursac N; Poss KD
[Ad] Endereço:Department of Cell Biology, Duke University Medical Center, Durham, NC 27710, USA; Regeneration Next, Duke University, Durham, NC 27710, USA.
[Ti] Título:Tension Creates an Endoreplication Wavefront that Leads Regeneration of Epicardial Tissue.
[So] Source:Dev Cell;42(6):600-615.e4, 2017 Sep 25.
[Is] ISSN:1878-1551
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Mechanisms that control cell-cycle dynamics during tissue regeneration require elucidation. Here we find in zebrafish that regeneration of the epicardium, the mesothelial covering of the heart, is mediated by two phenotypically distinct epicardial cell subpopulations. These include a front of large, multinucleate leader cells, trailed by follower cells that divide to produce small, mononucleate daughters. By using live imaging of cell-cycle dynamics, we show that leader cells form by spatiotemporally regulated endoreplication, caused primarily by cytokinesis failure. Leader cells display greater velocities and mechanical tension within the epicardial tissue sheet, and experimentally induced tension anisotropy stimulates ectopic endoreplication. Unbalancing epicardial cell-cycle dynamics with chemical modulators indicated autonomous regenerative capacity in both leader and follower cells, with leaders displaying an enhanced capacity for surface coverage. Our findings provide evidence that mechanical tension can regulate cell-cycle dynamics in regenerating tissue, stratifying the source cell features to improve repair.
[Mh] Termos MeSH primário: Endorreduplicação
Pericárdio/fisiologia
Regeneração
[Mh] Termos MeSH secundário: Animais
Fenômenos Biomecânicos
Movimento Celular
Células Gigantes/patologia
Hipertrofia
Camundongos Endogâmicos C57BL
Mitose
Poliploidia
Peixe-Zebra
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171030
[Lr] Data última revisão:
171030
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170927
[St] Status:MEDLINE


  6 / 7470 MEDLINE  
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[PMID]:28950096
[Au] Autor:Spiró Z; Heisenberg CP
[Ad] Endereço:Institute of Science and Technology Austria, Am Campus 1, 3400 Klosterneuburg, Austria.
[Ti] Título:Regeneration Tensed Up: Polyploidy Takes the Lead.
[So] Source:Dev Cell;42(6):559-560, 2017 09 25.
[Is] ISSN:1878-1551
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The cellular mechanisms allowing tissues to efficiently regenerate are not fully understood. In this issue of Developmental Cell, Cao et al. (2017) discover that during zebrafish heart regeneration, epicardial cells at the leading edge of regenerating tissue undergo endoreplication, possibly due to increased tissue tension, thereby boosting their regenerative capacity.
[Mh] Termos MeSH primário: Regeneração
Peixe-Zebra
[Mh] Termos MeSH secundário: Animais
Coração
Poliploidia
Proteínas de Peixe-Zebra/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; COMMENT
[Nm] Nome de substância:
0 (Zebrafish Proteins)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171030
[Lr] Data última revisão:
171030
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170927
[St] Status:MEDLINE


  7 / 7470 MEDLINE  
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[PMID]:28884266
[Au] Autor:Suo Y; Min Y; Dong C; Wang Y; Cheng S; Kang X
[Ad] Endereço:National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Technology, Beijing Forestry University, No. 35, Qinghua East Road, Beijing, 100083, People's Republic of China.
[Ti] Título:MicroRNA expression changes following synthesis of three full-sib Populus triploid populations with different heterozygosities.
[So] Source:Plant Mol Biol;95(3):215-225, 2017 Oct.
[Is] ISSN:1573-5028
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:KEY MESSAGE: Through high-throughput sequencing, we compared the relative expression levels of miRNA in three full-sib Populus triploid populations with that in their parents and one diploid hybrid population. We found similar numbers of miRNAs differentially expressed between the parents and the four progeny hybrid populations. In addition, unbalanced parental expression level dominance of miRNAs were found in the three allotriploid and interspecific hybrid populations, which may reprogram gene expression networks and contribute to the growth of Populus hybrids. These results indicated that hybridization has a great impact on the miRNA expression variation in the newly synthesized Populus triploid and diploid hybrid populations. However, we also found no significant differences in miRNA expression among one diploid and three triploid hybrid populations, hinting that miRNA abundances do not increase with the genome content. No dosage effect of miRNA expression could lead to dosage-dependent negative effects on target genes and their downstream pathway in polyploids. We speculate that polyploids may gain advantages from the slight decrease in miRNA regulation, suggesting an important molecular mechanism of polyploid advantage. Hybridization with three types of induced 2n gametes transmitted different parental heterozygosities has been proven as an efficient method for Populus triploid production. Several researches have shown that miRNA could be non-additively expressed in allopolyploids. However, it is still unclear whether the non-additively expressed miRNAs result from the effect of hybridization or polyploidization, and whether a dose response to the additional genomic content exists for the expression of miRNA. Toward this end, through high-throughput sequencing, we compared the expression levels of miRNA in three full-sib Populus triploid populations with that in their parents and one interspecific hybrid population. We found similar numbers of miRNAs differentially expressed between the parents and the four progeny hybrid populations. Unbalanced parental expression level dominance of miRNAs were found in the three triploid and diploid hybrid populations, which may reprogram gene expression networks and affect the growth of Populus hybrids. These results indicated that hybridization has a great impact on the miRNA expression variation in the newly synthesized Populus triploid and diploid hybrid populations. However, we also found no significant differences in miRNA expression among the three triploid populations and the diploid hybrid population. No dosage effect of miRNA expression could lead to dosage-dependent negative effects on target genes and their downstream pathway in polyploids. We speculate that polyploids may gain advantages from the decrease in miRNA negative regulation, suggesting an important molecular mechanism of polyploid advantage.
[Mh] Termos MeSH primário: Regulação da Expressão Gênica de Plantas
MicroRNAs/genética
Populus/genética
RNA de Plantas/genética
Triploidia
[Mh] Termos MeSH secundário: Cruzamentos Genéticos
Diploide
Perfilação da Expressão Gênica/métodos
Variação Genética
Genética Populacional
Heterozigoto
Sequenciamento de Nucleotídeos em Larga Escala/métodos
Hibridização Genética
Poliploidia
Reação em Cadeia da Polimerase Via Transcriptase Reversa
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (MicroRNAs); 0 (RNA, Plant)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171030
[Lr] Data última revisão:
171030
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170909
[St] Status:MEDLINE
[do] DOI:10.1007/s11103-017-0627-3


  8 / 7470 MEDLINE  
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[PMID]:28852802
[Au] Autor:van Geest G; Bourke PM; Voorrips RE; Marasek-Ciolakowska A; Liao Y; Post A; van Meeteren U; Visser RGF; Maliepaard C; Arens P
[Ad] Endereço:Plant Breeding, Wageningen University and Research, P.O. Box 386, 6708 PB, Wageningen, The Netherlands. geert.vangeest@wur.nl.
[Ti] Título:An ultra-dense integrated linkage map for hexaploid chrysanthemum enables multi-allelic QTL analysis.
[So] Source:Theor Appl Genet;130(12):2527-2541, 2017 Dec.
[Is] ISSN:1432-2242
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:KEY MESSAGE: We constructed the first integrated genetic linkage map in a polysomic hexaploid. This enabled us to estimate inheritance of parental haplotypes in the offspring and detect multi-allelic QTL. Construction and use of linkage maps are challenging in hexaploids with polysomic inheritance. Full map integration requires calculations of recombination frequency between markers with complex segregation types. In addition, detection of QTL in hexaploids requires information on all six alleles at one locus for each individual. We describe a method that we used to construct a fully integrated linkage map for chrysanthemum (Chrysanthemum × morifolium, 2n = 6x = 54). A bi-parental F1 population of 406 individuals was genotyped with an 183,000 SNP genotyping array. The resulting linkage map consisted of 30,312 segregating SNP markers of all possible marker dosage types, representing nine chromosomal linkage groups and 107 out of 108 expected homologues. Synteny with lettuce (Lactuca sativa) showed local colinearity. Overall, it was high enough to number the chrysanthemum chromosomal linkage groups according to those in lettuce. We used the integrated and phased linkage map to reconstruct inheritance of parental haplotypes in the F1 population. Estimated probabilities for the parental haplotypes were used for multi-allelic QTL analyses on four traits with different underlying genetic architectures. This resulted in the identification of major QTL that were affected by multiple alleles having a differential effect on the phenotype. The presented linkage map sets a standard for future genetic mapping analyses in chrysanthemum and closely related species. Moreover, the described methods are a major step forward for linkage mapping and QTL analysis in hexaploids.
[Mh] Termos MeSH primário: Mapeamento Cromossômico
Chrysanthemum/genética
Ligação Genética
Polimorfismo de Nucleotídeo Único
Locos de Características Quantitativas
[Mh] Termos MeSH secundário: Alelos
Marcadores Genéticos
Genoma de Planta
Técnicas de Genotipagem
Haplótipos
Alface/genética
Fenótipo
Poliploidia
Sintenia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Genetic Markers)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171117
[Lr] Data última revisão:
171117
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170831
[St] Status:MEDLINE
[do] DOI:10.1007/s00122-017-2974-5


  9 / 7470 MEDLINE  
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[PMID]:28835997
[Au] Autor:Lukaszewski AJ
[Ad] Endereço:Department of Botany and Plant Sci., University of California, Riverside, CA, 92521, USA. adam.lukaszewski@ucr.edu.
[Ti] Título:Chromosomes 1BS and 1RS for control of male fertility in wheats and triticales with cytoplasms of Aegilops kotschyi, Ae. mutica and Ae. uniaristata.
[So] Source:Theor Appl Genet;130(12):2521-2526, 2017 Dec.
[Is] ISSN:1432-2242
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:KEY MESSAGE: Engineered chromosomes 1BS and 1RS offer a new alternative in the development of hybrid systems in bread wheat and triticale. In the cytoplasmic male sterility system for hybrid wheat based on the cytoplasm of Triticum timopheevi fertility restoration is difficult, with few good restorer genes available. In the system based on the cytoplasms of Aegilops kotschyi, Ae. uniaristata and Ae. mutica, essentially all chromosomes 1B carry locus Rf that restores male fertility; male sterility manifests itself in wheats with the 1RS.1BL translocation where 1BS chromosome arm is missing. To generate male sterile wheats without the 1RS.1BL translocation, the 1BS arm was cytogenetically engineered to replace the segment with Rf with two short inserts of rye chromatin. Conversely, to enhance fertility restoration by doubling the number of restorers present for eventual use in wheat and triticale, a region of 1BS with Rf was inserted into 1RS. Alloplasmic wheats with Rf removed were completely male sterile; alloplasmic wheats with engineered 1RS carrying Rf and without normal 1B were male fertile. An exception to the ubiquitous presence of Rf is T. spelta var. duhamelianum; four accessions tested in this study gave inconsistent results but some did not restore male fertility. Engineered chromosomes 1BS and 1RS and chromosomes 1B of T. spelta offer a new alternative for practical application of a cytoplasmic male sterility system in the development of hybrid wheat and hexaploid triticale.
[Mh] Termos MeSH primário: Citoplasma/genética
Infertilidade das Plantas/genética
Triticale/genética
Triticum/genética
[Mh] Termos MeSH secundário: Cromossomos de Plantas
Engenharia Genética
Plantas Geneticamente Modificadas/genética
Plantas Geneticamente Modificadas/fisiologia
Poliploidia
Translocação Genética
Triticale/fisiologia
Triticum/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171109
[Lr] Data última revisão:
171109
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170825
[St] Status:MEDLINE
[do] DOI:10.1007/s00122-017-2973-6


  10 / 7470 MEDLINE  
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[PMID]:28821182
[Au] Autor:Panagiotopoulou H; Austin JD; Zalewska K; Gonciarz M; Czarnogórska K; Gawor J; Weglenski P; Popovic D
[Ad] Endereço:Museum and Institute of Zoology, Polish Academy of Sciences, Warsaw, Poland; Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland; Department of Wildlife Ecology and Conservation, University of Florida, Gainesville, FL 32611; Program in Fisheries and Aquatic Sciences,
[Ti] Título:Microsatellite Mutation Rate in Atlantic Sturgeon (Acipenser oxyrinchus).
[So] Source:J Hered;108(6):686-692, 2017 Sep 01.
[Is] ISSN:1465-7333
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Understanding mutation rates can greatly extend the utility of population and conservation genetic analyses. Herein, we present an estimate of genome-wide microsatellite mutation rate in Atlantic sturgeon (Acipenser oxyrinchus) based on parent-offspring transmission patterns. We screened 307 individuals for parentage and mutation-rate analysis applying 43 variable markers. Out of 13228 allele transfers, 11 mutations were detected, producing a mutation rate of 8.3 × 10-4 per locus per generation (95% confidence interval: 1.48 × 10-3, 4.15 × 10-4). Single-step mutations predominated and there were trends toward mutations in loci with greater polymorphism and allele length. Two of the detected mutations were most probably cluster mutations, being identified in 12 and 28 sibs, respectively. Finally, we observed evidences of polyploidy based on the sporadic presence of 3 or 4 alleles per locus in the genotyped individuals, supporting previous reports of incomplete diploidization in Atlantic sturgeon.
[Mh] Termos MeSH primário: Peixes/genética
Genética Populacional
Repetições de Microssatélites
Taxa de Mutação
[Mh] Termos MeSH secundário: Alelos
Animais
Feminino
Masculino
Poliploidia
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170914
[Lr] Data última revisão:
170914
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170820
[St] Status:MEDLINE
[do] DOI:10.1093/jhered/esx057



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