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  1 / 614 MEDLINE  
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[PMID]:29324803
[Au] Autor:Mangini G; Gadaleta A; Colasuonno P; Marcotuli I; Signorile AM; Simeone R; De Vita P; Mastrangelo AM; Laidò G; Pecchioni N; Blanco A
[Ad] Endereço:Department of Soil, Plant & Food Sciences, Genetics and Plant Breeding Section, University Aldo Moro, Bari, Italy.
[Ti] Título:Genetic dissection of the relationships between grain yield components by genome-wide association mapping in a collection of tetraploid wheats.
[So] Source:PLoS One;13(1):e0190162, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Increasing grain yield potential in wheat has been a major target of most breeding programs. Genetic advance has been frequently hindered by negative correlations among yield components that have been often observed in segregant populations and germplasm collections. A tetraploid wheat collection was evaluated in seven environments and genotyped with a 90K SNP assay to identify major and stable quantitative trait loci (QTL) for grain yield per spike (GYS), kernel number per spike (KNS) and thousand-kernel weight (TKW), and to analyse the genetic relationships between the yield components at QTL level. The genome-wide association analysis detected eight, eleven and ten QTL for KNS, TKW and GYS, respectively, significant in at least three environments or two environments and the mean across environments. Most of the QTL for TKW and KNS were found located in different marker intervals, indicating that they are genetically controlled independently by each other. Out of eight KNS QTL, three were associated to significant increases of GYS, while the increased grain number of five additional QTL was completely or partially compensated by decreases in grain weight, thus producing no or reduced effects on GYS. Similarly, four consistent and five suggestive TKW QTL resulted in visible increase of GYS, while seven additional QTL were associated to reduced effects in grain number and no effects on GYS. Our results showed that QTL analysis for detecting TKW or KNS alleles useful for improving grain yield potential should consider the pleiotropic effects of the QTL or the association to other QTLs.
[Mh] Termos MeSH primário: Genes de Plantas
Estudo de Associação Genômica Ampla
Tetraploidia
Triticum/genética
[Mh] Termos MeSH secundário: Locos de Características Quantitativas
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180129
[Lr] Data última revisão:
180129
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180112
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0190162


  2 / 614 MEDLINE  
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[PMID]:29262777
[Au] Autor:Lundström M; Leino MW; Hagenblad J
[Ad] Endereço:Linköping University, IFM Biology, SE-581 83, Linköping, Sweden.
[Ti] Título:Evolutionary history of the NAM-B1 gene in wild and domesticated tetraploid wheat.
[So] Source:BMC Genet;18(1):118, 2017 Dec 20.
[Is] ISSN:1471-2156
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: The NAM-B1 gene in wheat has for almost three decades been extensively studied and utilized in breeding programs because of its significant impact on grain protein and mineral content and pleiotropic effects on senescence rate and grain size. First detected in wild emmer wheat, the wild-type allele of the gene has been introgressed into durum and bread wheat. Later studies have, however, also found the presence of the wild-type allele in some domesticated subspecies. In this study we trace the evolutionary history of the NAM-B1 in tetraploid wheat species and evaluate it as a putative domestication gene. RESULTS: Genotyping of wild and landrace tetraploid accessions showed presence of only null alleles in durum. Domesticated emmer wheats contained both null alleles and the wild-type allele while wild emmers, with one exception, only carried the wild-type allele. One of the null alleles consists of a deletion that covers several 100 kb. The other null-allele, a one-basepair frame-shift insertion, likely arose among wild emmer. This allele was the target of a selective sweep, extending over several 100 kb. CONCLUSIONS: The NAM-B1 gene fulfils some criteria for being a domestication gene by encoding a trait of domestication relevance (seed size) and is here shown to have been under positive selection. The presence of both wild-type and null alleles in domesticated emmer does, however, suggest the gene to be a diversification gene in this species. Further studies of genotype-environment interactions are needed to find out under what conditions selection on different NAM-B1 alleles have been beneficial.
[Mh] Termos MeSH primário: Evolução Molecular
Proteínas de Plantas/genética
Triticum/classificação
Triticum/genética
[Mh] Termos MeSH secundário: Alelos
Evolução Biológica
Grãos Comestíveis
Variação Genética
Genótipo
Filogenia
Tetraploidia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Plant Proteins)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180118
[Lr] Data última revisão:
180118
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171222
[St] Status:MEDLINE
[do] DOI:10.1186/s12863-017-0566-7


  3 / 614 MEDLINE  
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[PMID]:28898255
[Au] Autor:Sulli M; Mandolino G; Sturaro M; Onofri C; Diretto G; Parisi B; Giuliano G
[Ad] Endereço:ENEA, Casaccia Research Center, Via Anguillarese 301, Roma, Italy.
[Ti] Título:Molecular and biochemical characterization of a potato collection with contrasting tuber carotenoid content.
[So] Source:PLoS One;12(9):e0184143, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:After wheat and rice, potato is the third most important staple food worldwide. A collection of ten tetraploid (Solanum tuberosum) and diploid (S. phureja and S. chacoense) genotypes with contrasting carotenoid content was subjected to molecular characterization with respect to candidate carotenoid loci and metabolic profiling using LC-HRMS. Irrespective of ploidy and taxonomy, tubers of these genotypes fell into three groups: yellow-fleshed, characterized by high levels of epoxy-xanthophylls and xanthophyll esters and by the presence of at least one copy of a dominant allele of the ß-Carotene Hydroxylase 2 (CHY2) gene; white-fleshed, characterized by low carotenoid levels and by the presence of recessive chy2 alleles; and orange-fleshed, characterized by high levels of zeaxanthin but low levels of xanthophyll esters, and homozygosity for a Zeaxanthin Epoxidase (ZEP) recessive allele. Novel CHY2 and ZEP alleles were identified in the collection. Multivariate analysis identified several groups of co-regulated non-polar compounds, and resulted in the grouping of the genotypes according to flesh color, suggesting that extensive cross-talk exists between the carotenoid pathway and other metabolite pathways in tubers. Postharvest traits like tuber dormancy and weight loss during storage showed little correlation with tuber carotenoid content, with the exception of zeaxanthin and its esters. Other tuber metabolites, such as glucose, monogalactosyldiacyglycerol (a glycolipid), or suberin precursors, showed instead significant correlations with both traits.
[Mh] Termos MeSH primário: Carotenoides/metabolismo
Tubérculos/metabolismo
Solanum tuberosum/genética
Solanum tuberosum/metabolismo
[Mh] Termos MeSH secundário: Alelos
Carotenoides/análise
Análise por Conglomerados
Diploide
Perfilação da Expressão Gênica
Regulação da Expressão Gênica de Plantas
Redes Reguladoras de Genes
Genes de Plantas
Estudos de Associação Genética
Genótipo
Metaboloma
Metabolômica/métodos
Fenótipo
Locos de Características Quantitativas
Tetraploidia
Xantofilas/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Xanthophylls); 36-88-4 (Carotenoids)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171018
[Lr] Data última revisão:
171018
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170913
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0184143


  4 / 614 MEDLINE  
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[PMID]:28789633
[Au] Autor:Ye L; Zhang C; Tang X; Chen Y; Liu S
[Ad] Endereço:State Key Laboratory of Developmental Biology of Freshwater Fish, College of Life Sciences, Hunan Normal University, Changsha, 410081, China.
[Ti] Título:Variations in 5S rDNAs in diploid and tetraploid offspring of red crucian carp × common carp.
[So] Source:BMC Genet;18(1):75, 2017 Aug 08.
[Is] ISSN:1471-2156
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: The allotetraploid hybrid fish (4nAT) that was created in a previous study through an intergeneric cross between red crucian carp (Carassius auratus red var., ♀) and common carp (Cyprinus carpio L., ♂) provided an excellent platform to investigate the effect of hybridization and polyploidization on the evolution of 5S rDNA. The 5S rDNAs of paternal common carp were made up of a coding sequence (CDS) and a non-transcribed spacer (NTS) unit, and while the 5S rDNAs of maternal red crucian carp contained a CDS and a NTS unit, they also contained a variable number of interposed regions (IPRs). The CDSs of the 5S rDNAs in both parental fishes were conserved, while their NTS units seemed to have been subjected to rapid evolution. RESULTS: The diploid hybrid 2nF inherited all the types of 5S rDNAs in both progenitors and there were no signs of homeologous recombination in the 5S rDNAs of 2nF by sequencing of PCR products. We obtained two segments of 5S rDNA with a total length of 16,457 bp from allotetraploid offspring 4nAT through bacterial artificial chromosome (BAC) sequencing. Using this sequence together with the 5S rDNA sequences amplified from the genomic DNA of 4nAT, we deduced that the 5S rDNAs of 4nAT might be inherited from the maternal progenitor red crucian carp. Additionally, the IPRs in the 5S rDNAs of 4nAT contained A-repeats and TA-repeats, which was not the case for the IPRs in the 5S rDNAs of 2nF . We also detected two signals of a 200-bp fragment of 5S rDNA in the chromosomes of parental progenitors and hybrid progenies by fluorescence in situ hybridization (FISH). CONCLUSIONS: We deduced that during the evolution of 5S rDNAs in different ploidy hybrid fishes, interlocus gene conversion events and tandem repeat insertion events might occurred in the process of polyploidization. This study provided new insights into the relationship among the evolution of 5S rDNAs, hybridization and polyploidization, which were significant in clarifying the genome evolution of polyploid fish.
[Mh] Termos MeSH primário: Carpas/genética
Diploide
Variação Genética
RNA Ribossômico 5S/genética
Tetraploidia
[Mh] Termos MeSH secundário: Animais
Sequência de Bases
Mapeamento Cromossômico
Hibridização in Situ Fluorescente
Homologia de Sequência
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Ribosomal, 5S)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171103
[Lr] Data última revisão:
171103
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170810
[St] Status:MEDLINE
[do] DOI:10.1186/s12863-017-0542-2


  5 / 614 MEDLINE  
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[PMID]:28750031
[Au] Autor:Deng M; Dong Y; Zhao Z; Li Y; Fan G
[Ad] Endereço:College of Forestry, Henan Agricultural University, Zhengzhou, Henan, P.R. China.
[Ti] Título:Dissecting the proteome dynamics of the salt stress induced changes in the leaf of diploid and autotetraploid Paulownia fortunei.
[So] Source:PLoS One;12(7):e0181937, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Exposure to high salinity can trigger acclimation in many plants. Such an adaptative response is greatly advantageous for plants and involves extensive reprogramming at the molecular level. Acclimation allows plants to survive in environments that are prone to increasing salinity. In this study, diploid and autotetraploid Paulownia fortunei seedlings were used to detect alterations in leaf proteins in plants under salt stress. Up to 152 differentially abundant proteins were identified by Multiplex run iTRAQ-based quantitative proteomic and LC-MS/MS methods. Bioinformatics analysis suggested that P. fortunei leaves reacted to salt stress through a combination of common responses, such as induced metabolism, signal transduction, and regulation of transcription. This study offers a better understanding of the mechanisms of salt tolerance in P. fortunei and provides a list of potential target genes that could be engineered for salt acclimation in plants, especially trees.
[Mh] Termos MeSH primário: Diploide
Lamiaceae/metabolismo
Lamiaceae/fisiologia
Folhas de Planta/fisiologia
Proteoma/metabolismo
Cloreto de Sódio/farmacologia
Estresse Fisiológico/efeitos dos fármacos
Tetraploidia
[Mh] Termos MeSH secundário: Análise por Conglomerados
Regulação da Expressão Gênica de Plantas/efeitos dos fármacos
Ontologia Genética
Marcação por Isótopo
Lamiaceae/efeitos dos fármacos
Lamiaceae/genética
Folhas de Planta/genética
Proteínas de Plantas/genética
Proteínas de Plantas/metabolismo
Proteômica
Reação em Cadeia da Polimerase em Tempo Real
Reprodutibilidade dos Testes
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Plant Proteins); 0 (Proteome); 451W47IQ8X (Sodium Chloride)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170929
[Lr] Data última revisão:
170929
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170728
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0181937


  6 / 614 MEDLINE  
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[PMID]:28732039
[Au] Autor:Wang J; Huo B; Liu W; Li D; Liao L
[Ad] Endereço:Beijing Advanced Innovation Centre for Tree Breeding by Molecular Design, Beijing Forestry University, Beijing, People's Republic of China.
[Ti] Título:Abnormal meiosis in an intersectional allotriploid of Populus L. and segregation of ploidy levels in 2x × 3x progeny.
[So] Source:PLoS One;12(7):e0181767, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Triploid plants are usually highly aborted owing to unbalanced meiotic chromosome segregation, but limited viable gametes can participate in the transition to different ploidy levels. In this study, numerous meiotic abnormalities were found with high frequency in an intersectional allotriploid poplar (Populus alba × P. berolinensis 'Yinzhong'), including univalents, precocious chromosome migration, lagging chromosomes, chromosome bridges, micronuclei, and precocious cytokinesis, indicating high genetic imbalance in this allotriploid. Some micronuclei trigger mini-spindle formation in metaphase II and participate in cytokinesis to form polyads with microcytes. Unbalanced chromosome segregation and chromosome elimination resulted in the formation of microspores with aneuploid chromosome sets. Fusion of sister nuclei occurs in microsporocytes with precocious cytokinesis, which could form second meiotic division restitution (SDR)-type gametes. However, SDR-type gametes likely contain incomplete chromosome sets due to unbalanced segregation of homologous chromosomes during the first meiotic division in triploids. Misorientation of spindles during the second meiotic division, such as fused and tripolar spindles with low frequency, could result in the formation of first meiotic division restitution (FDR)-type unreduced gametes, which most likely contain three complete chromosome sets. Although 'Yinzhong' yields 88.7% stainable pollen grains with wide diameter variation from 23.9 to 61.3 µm, the pollen viability is poor (2.78% ± 0.38). A cross of 'Yinzhong' pollen with a diploid female clone produced progeny with extensive segregation of ploidy levels, including 29 diploids, 18 triploids, 4 tetraploids, and 48 aneuploids, suggesting the formation of viable aneuploidy and unreduced pollen in 'Yinzhong'. Individuals with different chromosome compositions are potential to analyze chromosomal function and to integrate the chromosomal dosage variation into breeding programs of Populus.
[Mh] Termos MeSH primário: Segregação de Cromossomos/genética
Cromossomos de Plantas/genética
Meiose/genética
Populus/genética
[Mh] Termos MeSH secundário: Aneuploidia
Cruzamento/métodos
Diploide
Ploidias
Pólen/genética
Tetraploidia
Triploidia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170925
[Lr] Data última revisão:
170925
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170722
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0181767


  7 / 614 MEDLINE  
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[PMID]:28647100
[Au] Autor:Proctor L; Pradhan M; Leung S; Cheng A; Lee CH; Soslow RA; Gilks CB; Talhouk A; McAlpine JM; Danielsen HE; Hoang LN
[Ad] Endereço:Department of Gynecology and Obstetrics, University of British Columbia, Vancouver, British Columbia, Canada.
[Ti] Título:Assessment of DNA Ploidy in the ProMisE molecular subgroups of endometrial cancer.
[So] Source:Gynecol Oncol;146(3):596-602, 2017 Sep.
[Is] ISSN:1095-6859
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:OBJECTIVE: We sought to determine whether DNA ploidy correlates with the four molecular subgroups of endometrial carcinoma (EC) as determined using ProMisE (Proactive Molecular Risk Classifier for Endometrial Cancer). METHODS: 90 cases of EC previously characterized by clinicopathological parameters, outcomes, and ProMisE molecular subgroup (POLE EDM, MMR-D, p53 wt or p53 abn) were assessed for DNA ploidy using image cytometry. Associations of ploidy with traditional clinicopathological parameters were also tested. RESULTS: Abnormal DNA ploidy status differed amongst the ProMisE groups (p<0.001) and was found in 80.9% (17/21) of p53 abn, 37.0% (10/27) of p53 wt, 28.6% (4/14) of POLE EDM and 14.3% (4/28) of MMR-D. Abnormal DNA content was significantly associated with lower BMI (p=0.034) and grade 3 tumors (p=0.001). In the entire cohort, abnormal DNA content was significantly associated with worse progression free survival (p=0.0094) but not disease specific survival (p=0.249) or overall survival (p=0.187). When examining ploidy within each of the ProMisE groups, abnormal DNA content correlated with worse overall survival (p=0.041) and progression free survival (p=0.011) in the MMR-D group. No statistically significant relationship was seen in the remaining 3 groups. CONCLUSION: Abnormal DNA ploidy status did correlate with the molecular subgroups of EC; abnormal DNA content was seen in the large majority of p53 abn cases. Abnormal ploidy however was also seen in smaller numbers in the p53 wt, POLE EDM and MMR-D groups; therefore abnormal DNA content was not a specific marker for any one molecular group. The addition of ploidy to the ProMisE molecular categories conferred additional prognostic value within the MMR-D group, which merits further study.
[Mh] Termos MeSH primário: Carcinoma/genética
Carcinoma/patologia
DNA de Neoplasias/genética
Neoplasias do Endométrio/genética
Neoplasias do Endométrio/patologia
Ploidias
[Mh] Termos MeSH secundário: Idoso
Aneuploidia
Carcinoma/química
Reparo de Erro de Pareamento de DNA/genética
DNA Polimerase II/genética
Diploide
Intervalo Livre de Doença
Neoplasias do Endométrio/química
Feminino
Seres Humanos
Meia-Idade
Mutação
Gradação de Tumores
Invasividade Neoplásica
Estadiamento de Neoplasias
Proteínas de Ligação a Poli-ADP-Ribose
Receptores de Estradiol/análise
Receptores de Progesterona/análise
Taxa de Sobrevida
Tetraploidia
Proteína Supressora de Tumor p53/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Neoplasm); 0 (Poly-ADP-Ribose Binding Proteins); 0 (Receptors, Estradiol); 0 (Receptors, Progesterone); 0 (Tumor Suppressor Protein p53); EC 2.7.7.- (DNA Polymerase II); EC 2.7.7.7 (POLE protein, human)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170626
[St] Status:MEDLINE


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[PMID]:28605464
[Au] Autor:Patiño-Parrado I; Gómez-Jiménez Á; López-Sánchez N; Frade JM
[Ad] Endereço:Department of Molecular, Cellular, and Developmental Neurobiology, Cajal Institute, Consejo Superior de Investigaciones Científicas (IC-CSIC), Madrid E-28002, Spain.
[Ti] Título:Strand-specific CpG hemimethylation, a novel epigenetic modification functional for genomic imprinting.
[So] Source:Nucleic Acids Res;45(15):8822-8834, 2017 Sep 06.
[Is] ISSN:1362-4962
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Imprinted genes are regulated by allele-specific differentially DNA-methylated regions (DMRs). Epigenetic methylation of the CpGs constituting these DMRs is established in the germline, resulting in a 5-methylcytosine-specific pattern that is tightly maintained in somatic tissues. Here, we show a novel epigenetic mark, characterized by strand-specific hemimethylation of contiguous CpG sites affecting the germline DMR of the murine Peg3, but not Snrpn, imprinted domain. This modification is enriched in tetraploid cortical neurons, a cell type where evidence for a small proportion of formylmethylated CpG sites within the Peg3-controlling DMR is also provided. Single nucleotide polymorphism (SNP)-based transcriptional analysis indicated that these epigenetic modifications participate in the maintainance of the monoallelic expression pattern of the Peg3 imprinted gene. Our results unexpectedly demonstrate that the methylation pattern observed in DMRs controlling defined imprinting regions can be modified in somatic cells, resulting in a novel epigenetic modification that gives rise to strand-specific hemimethylated domains functional for genomic imprinting. We anticipate the existence of a novel molecular mechanism regulating the transition from fully methylated CpGs to strand-specific hemimethylated CpGs.
[Mh] Termos MeSH primário: Núcleo Celular/metabolismo
Metilação de DNA
Epigênese Genética
Impressão Genômica
Fatores de Transcrição Kruppel-Like/genética
[Mh] Termos MeSH secundário: 5-Metilcitosina/metabolismo
Alelos
Animais
Sequência de Bases
Núcleo Celular/genética
Córtex Cerebral/citologia
Córtex Cerebral/metabolismo
Ilhas de CpG
Embrião de Mamíferos
Fatores de Transcrição Kruppel-Like/metabolismo
Masculino
Camundongos
Camundongos Endogâmicos C57BL
Neurônios/citologia
Neurônios/metabolismo
Polimorfismo de Nucleotídeo Único
Alinhamento de Sequência
Tetraploidia
Transcrição Genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Kruppel-Like Transcription Factors); 0 (Peg3 protein, mouse); 6R795CQT4H (5-Methylcytosine)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171012
[Lr] Data última revisão:
171012
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170613
[St] Status:MEDLINE
[do] DOI:10.1093/nar/gkx518


  9 / 614 MEDLINE  
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[PMID]:28591168
[Au] Autor:Majka J; Ksiazczyk T; Kielbowicz-Matuk A; Kopecký D; Kosmala A
[Ad] Endereço:Institute of Plant Genetics, Polish Academy of Sciences, Poznan, Poland.
[Ti] Título:Exploiting repetitive sequences and BAC clones in Festuca pratensis karyotyping.
[So] Source:PLoS One;12(6):e0179043, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The Festuca genus is thought to be the most numerous genus of the Poaceae family. One of the most agronomically important forage grasses, Festuca pratensis Huds. is treated as a model plant to study the molecular mechanisms associated with tolerance to winter stresses, including frost. However, the precise mapping of the genes governing stress tolerance in this species is difficult as its karyotype remains unrecognized. Only two F. pratensis chromosomes with 35S and 5S rDNA sequences can be easily identified, but its remaining chromosomes have not been distinguished to date. Here, two libraries derived from F. pratensis nuclear DNA with various contents of repetitive DNA sequences were used as sources of molecular probes for fluorescent in situ hybridisation (FISH), a BAC library and a library representing sequences most frequently present in the F. pratensis genome. Using FISH, six groups of DNA sequences were revealed in chromosomes on the basis of their signal position, including dispersed-like sequences, chromosome painting-like sequences, centromeric-like sequences, knob-like sequences, a group without hybridization signals, and single locus-like sequences. The last group was exploited to develop cytogenetic maps of diploid and tetraploid F. pratensis, which are presented here for the first time and provide a remarkable progress in karyotype characterization.
[Mh] Termos MeSH primário: Cromossomos de Plantas/genética
Festuca/genética
Sequências Repetitivas de Ácido Nucleico/genética
Estresse Fisiológico/genética
[Mh] Termos MeSH secundário: Temperatura Baixa
Diploide
Festuca/crescimento & desenvolvimento
Biblioteca Gênica
Hibridização Genética
Hibridização in Situ Fluorescente
Cariotipagem
RNA Ribossômico 5S/genética
Tetraploidia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Ribosomal, 5S)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170913
[Lr] Data última revisão:
170913
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170608
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0179043


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[PMID]:28558380
[Au] Autor:Liu H; Sun B; Wang S; Liu C; Lu Y; Li D; Liu X
[Ad] Endereço:Department of Nuclear Medicine, Huashan Hospital, Fudan University, Shanghai, China.
[Ti] Título:Circulating Tumor Cells as a Biomarker in Pancreatic Ductal Adenocarcinoma.
[So] Source:Cell Physiol Biochem;42(1):373-382, 2017.
[Is] ISSN:1421-9778
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:BACKGROUND/AIMS: Circulating tumor cells (CTCs) are valuable in both basic research and clinical application for cancer management. In the current study, we evaluated the diagnostic value of CTCs in pancreatic ductal adenocarcinoma (PDAC). METHODS: In total, 143 blood samples from 95 consecutively diagnosed PDAC patients and 48 healthy donors were collected. Combined data from immunostaining of CD45, DAPI and fluorescence in situ hybridization (FISH) with chromosome 8 centromere (CEP8) probe were used to identify CTCs. Cells with features of CD45-/DAPI+/CEP8>2 were detected as CTCs. RESULTS: CTCs were classified as triploid, tetraploid and multiploid based on chromosome 8 copy number. CTC subtype composition was significantly different among groups. Both subtype number and total CTC number were significantly increased in PDAC patients, compared to healthy controls. Total CTC number had 75.8% sensitivity and 68.7% specificity at a cutoff value of 2 cells/3.2 mL. This study is the first to report that CTC subtype number is also useful in cancer diagnosis. Sensitivity was 53.7% and specificity was 85.4% at a cutoff point of 2 CTC subtypes. The diagnostic value of both total CTC number and CTC subtype number was a little poorer than CA199. CONCLUSIONS: Both CTC subtype and total CTC number may serve as potential biomarkers for PDAC.
[Mh] Termos MeSH primário: Biomarcadores Tumorais/metabolismo
Carcinoma Ductal Pancreático/diagnóstico
Células Neoplásicas Circulantes/metabolismo
Neoplasias Pancreáticas/diagnóstico
[Mh] Termos MeSH secundário: Idoso
Antígenos Glicosídicos Associados a Tumores/sangue
Área Sob a Curva
Carcinoma Ductal Pancreático/metabolismo
Carcinoma Ductal Pancreático/patologia
Estudos de Casos e Controles
Cromossomos Humanos Par 8/genética
Feminino
Seres Humanos
Hibridização in Situ Fluorescente
Antígenos Comuns de Leucócito/metabolismo
Medições Luminescentes
Masculino
Meia-Idade
Neoplasias Pancreáticas/metabolismo
Neoplasias Pancreáticas/patologia
Curva ROC
Tetraploidia
Triploidia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antigens, Tumor-Associated, Carbohydrate); 0 (Biomarkers, Tumor); 0 (carbohydrate antigen 199, human); EC 3.1.3.48 (Leukocyte Common Antigens)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170531
[St] Status:MEDLINE
[do] DOI:10.1159/000477481



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