Base de dados : MEDLINE
Pesquisa : C23.550.260.224.617.236 [Categoria DeCS]
Referências encontradas : 1426 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 143 ir para página                         

  1 / 1426 MEDLINE  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28433848
[Au] Autor:Ould-Slimane M; Damade C; Lonjon G; Gilibert A; Cochereau J; Gauthé R; Lonjon N
[Ad] Endereço:Spine Unit, Department of Orthopedic Surgery, Rouen University Hospital, Rouen, France.
[Ti] Título:Instrumented Circumferential Fusion in Two Stages for Instable Lumbar Fracture: Long-Term Results of a Series of 74 Patients on Sagittal Balance and Functional Outcomes.
[So] Source:World Neurosurg;103:303-309, 2017 Jul.
[Is] ISSN:1878-8769
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:OBJECTIVE: To report the radiologic and functional results of a multicenter, prospective case series of patients with comminuted lumbar fractures treated with 2-stage circumferential arthrodesis. METHODS: A multicenter prospective case series of 74 patients with comminuted lumbar fractures was analyzed. The strategy entailed initial posterior osteosynthesis, followed by physical replacement with an expandable titanium cage filled with autologous bone via retroperitoneal lumbotomy. The mechanism of lesion formation and epidemiologic characteristics were recorded. Clinical and quality-of-life analyses (visual analog scale [VAS], Oswesty Disability Index [ODI], Short Form 12 [SF-12]) were performed over a minimum observation period of 1 year. Radiologic parameters, including deformity measurements, were recorded at each evaluation. Fusion was analyzed by means of a 1-year monitoring scan. RESULTS: The mean patient age was 38.1 years, and median duration of follow-up was 2.1 years (interquartile range, 1.3-2.9). The distribution of fractures according to the Magerl classification scheme was as follows: A, 64.8%; B, 16.7%; C, 18.5%. At the last follow-up, fusion was considered certain in 57 cases (77%). The mean VAS score was 2.1 ± 1.3, mean ODI was 14.7 ± 8.0, mean SF-12 Physical Component Summary score was 43.2 ± 9.3, and mean SF-12 Mental Component Summary score was 50.8 ± 5.9. Correction of the regional sagittal deformity was significant during the postoperative period, with a mean increase in lordosis of 9.0° (P < 0.0001). The loss of mean correction at the last follow-up (-2.9°) was not significant. CONCLUSIONS: Circumferential arthrodesis, including posterior osteosynthesis and physical replacement with an expandable cage and autologous graft, is applicable to the treatment of comminuted lumbar fractures. A high rate of fusion was obtained with significant and long-lasting correction of the sagittal deformity. Functional scores measured at 1 year suggest mild disability. The ODI, SF-12, and VAS scores were positively correlated with fusion at the last follow-up.
[Mh] Termos MeSH primário: Transplante Ósseo/métodos
Fraturas Cominutivas/cirurgia
Instabilidade Articular/cirurgia
Vértebras Lombares/cirurgia
Equilíbrio Postural
Fraturas da Coluna Vertebral/cirurgia
Fusão Vertebral/métodos
[Mh] Termos MeSH secundário: Acidentes por Quedas
Acidentes de Trânsito
Adulto
Idoso
Autólise
Feminino
Seguimentos
Fraturas Cominutivas/diagnóstico por imagem
Fraturas não Consolidadas/epidemiologia
Seres Humanos
Vértebras Lombares/diagnóstico por imagem
Vértebras Lombares/lesões
Masculino
Meia-Idade
Análise Multivariada
Estudos Prospectivos
Qualidade de Vida
Fraturas da Coluna Vertebral/diagnóstico por imagem
Tomografia Computadorizada por Raios X
Resultado do Tratamento
Escala Visual Analógica
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170925
[Lr] Data última revisão:
170925
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170424
[St] Status:MEDLINE


  2 / 1426 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28159243
[Au] Autor:Comuzzo P; Calligaris S; Iacumin L; Ginaldi F; Voce S; Zironi R
[Ad] Endereço:Università degli Studi di Udine, Dipartimento di Scienze Agroalimentari, Ambientali ed Animali, via Sondrio 2/A, 33100 Udine, Italy. Electronic address: piergiorgio.comuzzo@uniud.it.
[Ti] Título:Application of multi-pass high pressure homogenization under variable temperature regimes to induce autolysis of wine yeasts.
[So] Source:Food Chem;224:105-113, 2017 Jun 01.
[Is] ISSN:0308-8146
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The effects of the number of passes and processing temperature management (controlled vs. uncontrolled) were investigated during high pressure homogenization-induced autolysis of Saccharomyces bayanus wine yeasts, treated at 150MPa. Both variables were able to affect cell viability, and the release of soluble molecules (free amino acids, proteins and glucidic colloids), but the effect of temperature was more important. S. bayanus cells were completely inactivated in 10 passes without temperature control (corresponding to a processing temperature of 75°C). The two processing variables also affected the volatile composition of the autolysates produced: higher temperatures led to a lower concentration of volatile compounds. The management of the operating conditions may allow the compositional characteristics of the products to be modulated, making them suitable for different winemaking applications.
[Mh] Termos MeSH primário: Fermentação/fisiologia
Temperatura Alta
Saccharomyces/metabolismo
Vinho/análise
[Mh] Termos MeSH secundário: Aminoácidos/metabolismo
Autólise
Cromatografia Gasosa-Espectrometria de Massas/métodos
Pressão
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Amino Acids)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170205
[St] Status:MEDLINE


  3 / 1426 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28119056
[Au] Autor:Serra F; Müller J; Gray J; Lüthi R; Dudas S; Czub S; Seuberlich T
[Ad] Endereço:NeuroCenter, Division of Neurological Sciences, Vetsuisse Faculty, University of Bern, Bern, Switzerland; Graduate School for Cellular and Biomedical Sciences, University of Bern, Bern, Switzerland. Electronic address: fabienne.serra@vetsuisse.unibe.ch.
[Ti] Título:PrP-C1 fragment in cattle brains reveals features of the transmissible spongiform encephalopathy associated PrP .
[So] Source:Brain Res;1659:19-28, 2017 Mar 15.
[Is] ISSN:1872-6240
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Three different types of bovine spongiform encephalopathy (BSE) are known and supposedly caused by distinct prion strains: the classical (C-) BSE type that was typically found during the BSE epidemic, and two relatively rare atypical BSE types, termed H-BSE and L-BSE. The three BSE types differ in the molecular phenotype of the disease associated prion protein, namely the N-terminally truncated proteinase K (PK) resistant prion protein fragment (PrP ). In this study, we report and analyze yet another PrP type (PrP ), which was found in severely autolytic brain samples of two cows in the framework of disease surveillance in Switzerland in 2011. Analysis of brain tissues from these animals by PK titration and PK inhibitor assays ruled out the process of autolysis as the cause for the aberrant PrP profile. Immunochemical characterization of the PrP fragments present in the 2011 cases by epitope mapping indicated that PrP corresponds in its primary sequence to the physiologically occurring PrP-C1 fragment. However, high speed centrifugation, sucrose gradient assay and NaPTA precipitation revealed biochemical similarities between PrP and the disease-associated prion protein found in BSE affected cattle in terms of detergent insolubility, PK resistance and PrP aggregation. Although it remains to be established whether PrP is associated with a transmissible disease, our results point out the need of further research on the role the PrP-C1 aggregation and misfolding in health and disease.
[Mh] Termos MeSH primário: Tronco Encefálico/química
Encefalopatia Espongiforme Bovina/metabolismo
Fragmentos de Peptídeos/análise
Proteínas PrPSc/agonistas
[Mh] Termos MeSH secundário: Envelhecimento/metabolismo
Animais
Autólise
Western Blotting
Tronco Encefálico/metabolismo
Bovinos
Detergentes/química
Encefalopatia Espongiforme Bovina/genética
Mapeamento de Epitopos
Fragmentos de Peptídeos/química
Fragmentos de Peptídeos/genética
Fragmentos de Peptídeos/metabolismo
Proteínas PrPSc/química
Proteínas PrPSc/genética
Proteínas PrPSc/metabolismo
Agregados Proteicos
Solubilidade
Suíça
Ultracentrifugação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Detergents); 0 (Peptide Fragments); 0 (PrPSc Proteins); 0 (Protein Aggregates)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170807
[Lr] Data última revisão:
170807
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170126
[St] Status:MEDLINE


  4 / 1426 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
[PMID]:27834531
[Au] Autor:Atkin L; Rippon M
[Ad] Endereço:Vascular Nurse Specialist/Lecturer Practitioner, Mid Yorks NHS Trust/University of Huddersfield.
[Ti] Título:Autolysis: mechanisms of action in the removal of devitalised tissue.
[So] Source:Br J Nurs;25(20 Suppl):S40-S47, 2016 Nov 10.
[Is] ISSN:0966-0461
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Chronic wounds affect millions of people worldwide. In the UK alone, the cost of their treatment is estimated to be between £4.5bn and £5.1bn. The implementation of wound-bed preparation strategies remove the barriers to healing and wound debridement is a key component in preparing the wound bed for wound progression. This article aims to review one of the several debridement methods available to clinicians: autolytic debridement. Autolysis (i.e. autolytic debridement) uses the body's own enzymatic mechanisms to remove devitalised tissue in order to remove the barriers to healing. This review aims to provide clinicians working in wound care with a better understanding of the mechanisms and implications of autolytic debridement.
[Mh] Termos MeSH primário: Alginatos/uso terapêutico
Autólise
Curativos Hidrocoloides
Desbridamento/métodos
Cicatrização
Ferimentos e Lesões/enfermagem
[Mh] Termos MeSH secundário: Coloides/uso terapêutico
Seres Humanos
Hidrogéis/uso terapêutico
Ferimentos e Lesões/terapia
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Alginates); 0 (Colloids); 0 (Hydrogels)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170214
[Lr] Data última revisão:
170214
[Sb] Subgrupo de revista:N
[Da] Data de entrada para processamento:161112
[St] Status:MEDLINE


  5 / 1426 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
[PMID]:27829373
[Au] Autor:Bryant JC; Dabbs RC; Oswalt KL; Brown LR; Rosch JW; Seo KS; Donaldson JR; McDaniel LS; Thornton JA
[Ad] Endereço:Department of Biological Sciences, Mississippi State University, 295 E Lee Blvd., Harned Hall, Rm 219, Mississippi State, MS, 39762, USA.
[Ti] Título:Pyruvate oxidase of Streptococcus pneumoniae contributes to pneumolysin release.
[So] Source:BMC Microbiol;16(1):271, 2016 Nov 09.
[Is] ISSN:1471-2180
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Streptococcus pneumoniae is one of the leading causes of community acquired pneumonia and acute otitis media. Certain aspects of S. pneumoniae's virulence are dependent upon expression and release of the protein toxin pneumolysin (PLY) and upon the activity of the peroxide-producing enzyme, pyruvate oxidase (SpxB). We investigated the possible synergy of these two proteins and identified that release of PLY is enhanced by expression of SpxB prior to stationary phase growth. RESULTS: Mutants lacking the spxB gene were defective in PLY release and complementation of spxB restored PLY release. This was demonstrated by cytotoxic effects of sterile filtered supernatants upon epithelial cells and red blood cells. Additionally, peroxide production appeared to contribute to the mechanism of PLY release since a significant correlation was found between peroxide production and PLY release among a panel of clinical isolates. Exogenous addition of H O failed to induce PLY release and catalase supplementation prevented PLY release in some strains, indicating peroxide may exert its effect intracellularly or in a strain-dependent manner. SpxB expression did not trigger bacterial cell death or LytA-dependent autolysis, but did predispose cells to deoxycholate lysis. CONCLUSIONS: Here we demonstrate a novel link between spxB expression and PLY release. These findings link liberation of PLY toxin to oxygen availability and pneumococcal metabolism.
[Mh] Termos MeSH primário: Piruvato Oxidase/metabolismo
Streptococcus pneumoniae/metabolismo
Estreptolisinas/metabolismo
[Mh] Termos MeSH secundário: Autólise
Proteínas de Bactérias/genética
Proteínas de Bactérias/metabolismo
Catalase
DNA Bacteriano/genética
Células Epiteliais/microbiologia
Eritrócitos/microbiologia
Genes Bacterianos
Peróxido de Hidrogênio/metabolismo
Oxigênio
Piruvato Oxidase/genética
Deleção de Sequência
Streptococcus pneumoniae/enzimologia
Streptococcus pneumoniae/genética
Estreptolisinas/genética
Virulência
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (DNA, Bacterial); 0 (Streptolysins); 0 (plY protein, Streptococcus pneumoniae); BBX060AN9V (Hydrogen Peroxide); EC 1.11.1.6 (Catalase); EC 1.2.3.3 (Pyruvate Oxidase); S88TT14065 (Oxygen)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170626
[Lr] Data última revisão:
170626
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161111
[St] Status:MEDLINE


  6 / 1426 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27451206
[Au] Autor:Liu R; Li Y; Wang M; Zhou G; Zhang W
[Ad] Endereço:Key Laboratory of Meat Processing and Quality Control, Ministry of Education China, Jiangsu Collaborative Innovation Center of Meat Production and Processing, Quality and Safety Control, College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
[Ti] Título:Effect of protein S-nitrosylation on autolysis and catalytic ability of µ-calpain.
[So] Source:Food Chem;213:470-477, 2016 Dec 15.
[Is] ISSN:0308-8146
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The effect of S-nitrosylation on the autolysis and catalytic ability of µ-calpain in vitro in the presence of 50µM Ca(2 +) was investigated. µ-Calpain was incubated with different concentrations of nitric oxide donor S-nitrosoglutathione (GSNO) and subsequently reacted with purified myofibrils. Results showed that the amount of 80kDa µ-calpain subunit significantly decreased as GSNO increased from 0 to 300µM, but increases of GSNO to 300, 500 and 1000µM did not result in further inhibition. The catalytic ability of nitrosylated µ-calpain to degrade titin, nebulin, troponin-T and desmin was significantly reduced when the GSNO concentration was higher than 300µM. The cysteine residues of µ-calpain at positions 49, 351, 384, and 592 in the catalytic subunit and at 142 in small subunit were S-nitrosylated, which could be responsible for decreased µ-calpain activity. Thus, S-nitrosylation can negatively regulate the activation of µ-calpain resulting in decreased proteolytic ability on myofibrils.
[Mh] Termos MeSH primário: Autólise/metabolismo
Calpaína/metabolismo
Proteína S/metabolismo
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Calpaína/análise
Calpaína/genética
Catálise
Desmina/análise
Desmina/genética
Desmina/metabolismo
Proteínas Musculares/análise
Proteínas Musculares/genética
Proteínas Musculares/metabolismo
Miofibrilas/metabolismo
Proteína S/análise
Proteína S/genética
Proteólise
Suínos
Troponina T/análise
Troponina T/genética
Troponina T/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Desmin); 0 (Muscle Proteins); 0 (Protein S); 0 (Troponin T); 0 (nebulin); EC 3.4.22.- (Calpain); EC 3.4.22.- (mu-calpain)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170809
[Lr] Data última revisão:
170809
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160725
[St] Status:MEDLINE


  7 / 1426 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27374541
[Au] Autor:Liu YX; Zhou DY; Ma DD; Liu YF; Li DM; Dong XP; Tan MQ; Du M; Zhu BW
[Ad] Endereço:School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, PR China; National Engineering Research Center of Seafood, Dalian 116034, PR China.
[Ti] Título:Changes in collagenous tissue microstructures and distributions of cathepsin L in body wall of autolytic sea cucumber (Stichopus japonicus).
[So] Source:Food Chem;212:341-8, 2016 Dec 01.
[Is] ISSN:0308-8146
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The autolysis of sea cucumber (Stichopus japonicus) was induced by ultraviolet (UV) irradiation, and the changes of microstructures of collagenous tissues and distributions of cathepsin L were investigated using histological and histochemical techniques. Intact collagen fibers in fresh S. japonicus dermis were disaggregated into collagen fibrils after UV stimuli. Cathepsin L was identified inside the surface of vacuoles in the fresh S. japonicus dermis cells. After the UV stimuli, the membranes of vacuoles and cells were fused together, and cathepsin L was released from cells and diffused into tissues. The density of cathepsin L was positively correlated with the speed and degree of autolysis in different layers of body wall. Our results revealed that lysosomal cathepsin L was released from cells in response to UV stimuli, which contacts and degrades the extracellular substrates such as collagen fibers, and thus participates in the autolysis of S. japonicus.
[Mh] Termos MeSH primário: Autólise/patologia
Catepsina L/metabolismo
Colágeno/química
Pepinos-do-Mar/química
Raios Ultravioleta
[Mh] Termos MeSH secundário: Animais
Colágeno/ultraestrutura
Microscopia Eletrônica de Varredura
Pepinos-do-Mar/efeitos da radiação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
9007-34-5 (Collagen); EC 3.4.22.15 (Cathepsin L)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170117
[Lr] Data última revisão:
170117
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160705
[St] Status:MEDLINE


  8 / 1426 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27352439
[Au] Autor:Cocariu EA; Mageriu V; Staniceanu F; Bastian A; Socoliuc C; Zurac S
[Ti] Título:Correlations Between the Autolytic Changes and Postmortem Interval in Refrigerated Cadavers.
[So] Source:Rom J Intern Med;54(2):105-12, 2016 Apr-Jun.
[Is] ISSN:1220-4749
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:INTRODUCTION: In forensic pathology the autolytic process has been observed and documented in order to determine the postmortem interval as accurately as possible. The observation and experiments have been carried out on cadavers exposed to environmental conditions - heat, humidity, air currents, soil, water. METHODS: For this study hematoxylin and eosin (HE) stained sections of organ samples from 30 autopsied bodies were examined under the microscope. Modifications of tissue and cell structures were noted in correlation with the bodies' time spent in the Morgue's mortuary refrigerator until the autopsy was performed, which varied between 24 hours and 22 days. RESULTS: All the organs sampled (lung, heart, liver and pancreas) showed severe autolytic alterations after 5 to 8 days. The most heavily affected was the pancreas, cells within Langherhans islets becoming complete autolyzed at the 36 hours mark. Inside organs, autolytic processes occur at different rates depending on the locations within that organ -deeply or superficial; in the heart after 4 or more days subendocardic myocardium shows less severe autolytic changes than the subepicardial one. CONCLUSION: Autolytic processes have a delayed onset and a much lower progression rate in a cold controlled environment. Different organs suffer different rates of autolysis in correlation to their structure and enzymatic content.
[Mh] Termos MeSH primário: Autólise
Cadáver
Erros de Diagnóstico/prevenção & controle
Patologia Legal
Refrigeração
[Mh] Termos MeSH secundário: Seres Humanos
Fígado/patologia
Pulmão/patologia
Miocárdio/patologia
Pâncreas/patologia
Mudanças Depois da Morte
Estudos Retrospectivos
Fatores de Tempo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1609
[Cu] Atualização por classe:160629
[Lr] Data última revisão:
160629
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160629
[St] Status:MEDLINE


  9 / 1426 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
[PMID]:27302406
[Au] Autor:Hajnal I; Chen X; Chen GQ
[Ad] Endereço:Peking-Tsinghua Center for Life Sciences, School of Life Science, Tsinghua University, Beijing, 100084, China.
[Ti] Título:A novel cell autolysis system for cost-competitive downstream processing.
[So] Source:Appl Microbiol Biotechnol;100(21):9103-9110, 2016 Nov.
[Is] ISSN:1432-0614
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:The industrial production of low value-added biological products poses significant challenges due to cost pressures. In recent years, it has been argued that synthetic biology approaches will lead to breakthroughs that eliminate price bottlenecks for the production of a wide range of biological products including bioplastics and biofuels. One significant bottleneck lies in the necessity to break the tough cell walls of microbes in order to release intracellular products. We here report the implementation of the first synthetic biology standard part based on the lambda phage SRRz genes and a synthetic ribosome binding site (RBS) that works in Escherichia coli and Halomonas campaniensis, which enables the producer strains to induce lysis after the addition of small amounts (1-5 %) of solvents or to spontaneously lyse during the stresses of downstream processing, and thus has the potential to eliminate the mechanical cell disruption step as both an efficiency bottleneck and a significant capex barrier when implementing downstream bioprocesses.
[Mh] Termos MeSH primário: Autólise
Bacteriólise
Produtos Biológicos/isolamento & purificação
Biotecnologia/métodos
Escherichia coli/metabolismo
Halomonas/metabolismo
[Mh] Termos MeSH secundário: Bacteriófago lambda/genética
Biotecnologia/economia
Custos e Análise de Custo
Escherichia coli/genética
Genes Virais
Halomonas/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biological Products)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170118
[Lr] Data última revisão:
170118
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160616
[St] Status:MEDLINE


  10 / 1426 MEDLINE  
              first record previous record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27156898
[Au] Autor:Finnie JW; Blumbergs PC; Manavis J
[Ad] Endereço:SA Pathology, Hanson Institute Centre for Neurological Diseases and Schools of Medicine and Veterinary Science, University of Adelaide, Adelaide, South Australia, Australia. Electronic address: john.finnie@sa.gov.au.
[Ti] Título:Temporal Sequence of Autolysis in the Cerebellar Cortex of the Mouse.
[So] Source:J Comp Pathol;154(4):323-8, 2016 May.
[Is] ISSN:1532-3129
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:This study examined the temporal sequence of post-mortem changes in the cerebellar cortical granular and Purkinje cell layers of mice kept at a constant ambient temperature for up to 4 weeks. Nuclei of granule cell microneurons became pyknotic early after death, increasing progressively until, by 7 days, widespread nuclear lysis resulted in marked cellular depletion of the granular layer. Purkinje cells were relatively unaltered until about 96 h post mortem, at which time there was shrinkage and multivacuolation of the amphophilic cytoplasm, nuclear hyperchromasia and, sometimes, a perinuclear clear space. By 7 days, Purkinje cells had hypereosinophilic cytoplasm and frequent nuclear pyknosis. By 2 weeks after death, Purkinje cells showed homogenization, the cytoplasm being uniformly eosinophilic, progressing to a 'ghost-like' appearance in which the cytoplasm had pale eosinophilic staining with indistinct cell boundaries, and nuclei often absent. The results of this study could assist in differentiating post-mortem autolysis from ante-mortem lesions in the cerebellar cortex and determining the post-mortem interval. Moreover, this information could be useful when interpreting brain lesions in valuable mice found dead unexpectedly during the course of biomedical experiments.
[Mh] Termos MeSH primário: Autólise/patologia
Córtex Cerebelar/patologia
Células de Purkinje/patologia
[Mh] Termos MeSH secundário: Animais
Feminino
Imuno-Histoquímica
Camundongos
Neuroglia/patologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170414
[Lr] Data última revisão:
170414
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160510
[St] Status:MEDLINE



página 1 de 143 ir para página                         
   


Refinar a pesquisa
  Base de dados : MEDLINE Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde