| [PMID]: | 29331375 |
| [Au] Autor: | Ma L; Wang Q; Yuan M; Zou T; Yin P; Wang S |
| [Ad] Endereço: | National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan 430070, China. |
| [Ti] Título: | Xanthomonas TAL effectors hijack host basal transcription factor IIA α and γ subunits for invasion. |
| [So] Source: | Biochem Biophys Res Commun;496(2):608-613, 2018 02 05. |
| [Is] ISSN: | 1090-2104 |
| [Cp] País de publicação: | United States |
| [La] Idioma: | eng |
| [Ab] Resumo: | The Xanthomonas genus includes Gram-negative plant-pathogenic bacteria, which infect a broad range of crops and wild plant species, cause symptoms with leaf blights, streaks, spots, stripes, necrosis, wilt, cankers and gummosis on leaves, stems and fruits in a wide variety of plants via injecting their effector proteins into the host cell during infection. Among these virulent effectors, transcription activator-like effectors (TALEs) interact with the γ subunit of host transcription factor IIA (TFIIAγ) to activate the transcription of host disease susceptibility genes. Functional TFIIA is a ternary complex comprising α, ß and γ subunits. However, whether TALEs recruit TFIIAα, TFIIAß, or both remains unknown. The underlying molecular mechanisms by which TALEs mediate host susceptibility gene activation require full elucidation. Here, we show that TALEs interact with the α+γ binary subcomplex but not the α+ß+γ ternary complex of rice TFIIA (holo-OsTFIIA). The transcription factor binding (TFB) regions of TALEs, which are highly conserved in Xanthomonas species, have a dominant role in these interactions. Furthermore, the interaction between TALEs and the α+γ complex exhibits robust DNA binding activity in vitro. These results collectively demonstrate that TALE-carrying pathogens hijack the host basal transcription factors TFIIAα and TFIIAγ, but not TFIIAß, to enhance host susceptibility during pathogen infection. The uncovered mechanism widens new insights on host-microbe interaction and provide an applicable strategy to breed high-resistance crop varieties. |
| [Mh] Termos MeSH primário: |
Interações Hospedeiro-Patógeno
Oryza/microbiologia
Doenças das Plantas/microbiologia
Proteínas de Plantas/metabolismo
Efetores Semelhantes a Ativadores de Transcrição/metabolismo
Fator de Transcrição TFIIA/metabolismo
Xanthomonas/fisiologia
|
| [Mh] Termos MeSH secundário: |
Resistência à Doença
Regulação da Expressão Gênica de Plantas
Genes de Plantas
Oryza/genética
Oryza/metabolismo
Doenças das Plantas/genética
Ligação Proteica
Subunidades Proteicas/metabolismo
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| [Pt] Tipo de publicação: | JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T |
[Nm] Nome de substância:
| 0 (Plant Proteins); 0 (Protein Subunits); 0 (Transcription Activator-Like Effectors); 0 (Transcription Factor TFIIA) |
| [Em] Mês de entrada: | 1803 |
| [Cu] Atualização por classe: | 180309 |
[Lr] Data última revisão:
| 180309 |
| [Sb] Subgrupo de revista: | IM |
| [Da] Data de entrada para processamento: | 180115 |
| [St] Status: | MEDLINE |
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