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[PMID]:28457516
[Au] Autor:Nakata M; Kasuda S; Yuui K; Kudo R; Hatake K
[Ad] Endereço:Department of Legal Medicine, Nara Medical University, 840 Shijo-cho, Kashihara, Nara 634-8521, Japan. Electronic address: dc112064@naramed-u.ac.jp.
[Ti] Título:Relevance of hemolysis-induced tissue factor expression on monocytes in soft clot formation in alcohol-containing blood.
[So] Source:Leg Med (Tokyo);25:83-88, 2017 Mar.
[Is] ISSN:1873-4162
[Cp] País de publicação:Ireland
[La] Idioma:eng
[Ab] Resumo:The fluidity of cadaveric blood is an important characteristic in the post-mortem examination of cases of asphyxial death. Although it is empirically known that soft blood clots are present in cadaveric blood containing alcohol, the relationship between such clots and blood alcohol is unclear. We addressed this issue through in vitro studies using blood collected from healthy volunteers. Assessment of global hemostasis by rotational thromboelastometry revealed that ethanol treatment enhanced the procoagulant activity of whole blood. However, ethanol inhibited epinephrine-induced platelet aggregation, whereas plasma levels of von Willebrand factor and the activity of coagulation factors VIII and IX were unaffected. In contrast, tissue factor (TF) activity was higher in plasma obtained from ethanol-treated whole blood than that in plasma from untreated blood. Ethanol induced hemolysis of red blood cells, and the consequent hemoglobin (Hb) release promoted de novo synthesis of TF in isolated monocytes, as determined by real-time reverse transcription PCR, western blotting, and flow cytometry. However, ethanol itself did not induce TF expression in monocytes. Given that TF activates the extrinsic coagulation pathway and amplifies hemostatic reactions, Hb-induced TF expression in monocytes might contribute to soft blood clot formation.
[Mh] Termos MeSH primário: Coagulação Sanguínea/efeitos dos fármacos
Etanol/sangue
Hemólise
Monócitos/efeitos dos fármacos
Tromboplastina/efeitos dos fármacos
[Mh] Termos MeSH secundário: Autopsia
Cadáver
Citometria de Fluxo
Medicina Legal
Seres Humanos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
3K9958V90M (Ethanol); 9035-58-9 (Thromboplastin)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180307
[Lr] Data última revisão:
180307
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170502
[St] Status:MEDLINE


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[PMID]:27775924
[Au] Autor:Im SH; Jung Y; Jang Y; Kim SH
[Ad] Endereço:KU-KIST Graduate School of Converging Science and Technology, Korea University, 145 Anam-ro, Seongbuk-gu, Seoul, 02841, Korea. Biomaterials Research Center, Korea Institute of Science and Technology (KIST), Seoul 02792, Korea.
[Ti] Título:Poly(L-lactic acid) scaffold with oriented micro-valley surface and superior properties fabricated by solid-state drawing for blood-contact biomaterials.
[So] Source:Biofabrication;8(4):045010, 2016 10 24.
[Is] ISSN:1758-5090
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Most biomaterials composed of biodegradable polymers will contact either accidentally or consistently with blood and this commonly requires both good  mechanical strength and blood compatibility. Despite this demand, current processing methods still make it difficult and complex to simultaneously improve the two properties. To overcome present limitations, the aim of this work is to develop a solid-state drawing which is a novel method for blood-contact biomaterials that can simultaneously improve the two essential factors of mechanical strength and blood compatibility, as well as induce a micro-patterned surface. Solid-state drawn (SSD) poly(L-lactic acid) (PLLA) film significantly maximally increased tensile strength and elastic modulus about ninefold and sixfold, respectively, compared to undrawn film. Furthermore, it was determined that SSD-PLLA film had highly developed molecular orientation, higher crystallinity and surface hydrophobicity. Additionally, the SSD method could greatly reduce roughness of the surface and induce the formation of aligned valleys, forming microstructures on the film surface. The topographical cue delayed hydrolytic degradation and prevented damage on the surface by NaOH of alkali compounds are compared with undrawn film. In energy-dispersive x-ray spectroscopy analysis, the surface of SSD film treated by NaOH was not detected on any ions whereas undrawn film held foreign ions on surface defects. The hemolysis rate of SSD film was considerably decreased with an increase of draw ratio up to 0.2% maximally and SSD film has shown greatly lower platelet adhesion compared to undrawn film in blood-compatibility analysis. Interestingly, one-directional alignment of micro-valley structure on SSD film could promote initial adhesion of human umbilical vein endothelial cells (HUVEC) compared with undrawn film and guide the direction of HUVEC. In conclusion, the newly designed SSD method has shown potential for developing blood-contact biomaterials simply due to great mechanical properties, blood compatibility and an aligned micro-patterned surface.
[Mh] Termos MeSH primário: Materiais Biocompatíveis/química
Poliésteres/química
[Mh] Termos MeSH secundário: Materiais Biocompatíveis/farmacologia
Plaquetas/citologia
Varredura Diferencial de Calorimetria
Adesão Celular/efeitos dos fármacos
Módulo de Elasticidade
Eritrócitos/citologia
Eritrócitos/efeitos dos fármacos
Hemólise/efeitos dos fármacos
Células Endoteliais da Veia Umbilical Humana
Seres Humanos
Microscopia de Força Atômica
Espectrometria por Raios X
Propriedades de Superfície
Resistência à Tração
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Biocompatible Materials); 0 (Polyesters); 459TN2L5F5 (poly(lactide))
[Em] Mês de entrada:1711
[Cu] Atualização por classe:180308
[Lr] Data última revisão:
180308
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161025
[St] Status:MEDLINE


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[PMID]:28470926
[Au] Autor:Lehle K; Lubnow M; Philipp A; Foltan M; Zeman F; Zausig Y; Lunz D; Schmid C; Müller T
[Ad] Endereço:Department of Cardiothoracic Surgery, University Hospital Regensburg, Franz-Josef-Strauss-Allee 11, 93042, Regensburg, Germany.
[Ti] Título:Prevalence of hemolysis and metabolic acidosis in patients with circulatory failure supported with extracorporeal life support: a marker for survival?
[So] Source:Eur J Heart Fail;19 Suppl 2:110-116, 2017 May.
[Is] ISSN:1879-0844
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:AIMS: Elevated levels of plasma free hemoglobin (fHb) indicate red blood cell (RBC) damage. The aim of this study was to analyze the prevalence of hemolysis and metabolic acidosis in patients on extracorporeal life support (ECLS) and to investigate whether it is a marker for outcome. METHODS AND RESULTS: This retrospective analysis included 215 adult patients with cardiac failure treated with ECLS. The cohort was divided into three groups: ECLS (1) during ongoing cardiopulmonary resuscitation (CPR, n = 110); (2) after CPR with return of spontaneous circulation and sustained cardiogenic shock (n = 45); (3) in severe cardiogenic shock without previous CPR (n = 60). Lactate, arterial pH value and fHb were measured daily before (pre-fHb) and during ECLS. CPR caused a pronounced increase in pre-fHb (group1, 318 (138/586) mg/L; group2, 212 (107/439) mg/L; group3, 79 (53/232) mg/L; p < 0.001). Within 24 hours on ECLS, fHb declined significantly. Compared to group 3 without CPR, group1 and 2 had a lower pH value (group1, 7.10 (6.93/7.20); group2, 7.21 (7.16/7.27); group3, 7.28 (7.20/7.35); p < 0.001), and an increased lactate level (group1, 88 (55/129) mg/dL; group2, 76 (36/111) mg/dL; group3, 52 (25/83) mg/dL; p < 0.0001). Multivariante analysis showed that pre-fHb had no prognostic value for survival. Only a low pre-lactate was a surrogate marker for successful weaning (p < 0.0001) and discharge from hospital (p = 0.0028). CONCLUSIONS: CPR was associated with a strongly increased fHb irrespective of ECLS. Implantation of ECLS did not aggravate hemolysis but instead decreased it within 24 hours. In this study low pre-fHb had no predictive value for survival.
[Mh] Termos MeSH primário: Acidose/epidemiologia
Anemia Hemolítica/epidemiologia
Oxigenação por Membrana Extracorpórea/efeitos adversos
Insuficiência Cardíaca/terapia
Hemólise
[Mh] Termos MeSH secundário: Acidose/sangue
Acidose/etiologia
Idoso
Anemia Hemolítica/sangue
Anemia Hemolítica/etiologia
Feminino
Alemanha/epidemiologia
Hemoglobinas/metabolismo
Seres Humanos
Masculino
Meia-Idade
Prevalência
Prognóstico
Estudos Retrospectivos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Hemoglobins)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180305
[Lr] Data última revisão:
180305
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170505
[St] Status:MEDLINE
[do] DOI:10.1002/ejhf.854


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[PMID]:29329071
[Au] Autor:de Santana TI; Barbosa MO; Gomes PATM; da Cruz ACN; da Silva TG; Leite ACL
[Ad] Endereço:Departamento de Antibióticos, Centro de Biociências, Universidade Federal de Pernambuco, 50740-520, Recife, PE, Brazil.
[Ti] Título:Synthesis, anticancer activity and mechanism of action of new thiazole derivatives.
[So] Source:Eur J Med Chem;144:874-886, 2018 Jan 20.
[Is] ISSN:1768-3254
[Cp] País de publicação:France
[La] Idioma:eng
[Ab] Resumo:Thiazole derivatives are recognized to possess various biological activities as antiparasitic, antifungal, antimicrobial and antiproliferative. The present work reports the synthesis of 22 new substances belonging to two classes of compounds: thiosemicarbazones and thiazoles, with the purpose of developing new drugs that present high specificity for tumor cells and low toxicity to the organism. A cytotoxic screening was performed to evaluate the performance of the new derivatives in five tumor cell lines. Eight compounds were shown to be promising in at least three tumor cell lines. These compounds had their IC determined within 72 h and the activity structure ratio was assessed. The effect of the best compounds on PBMC and hemolytic activity assay was then evaluated. The compound 1d was considered the most promising among the samples tested and its influence on cell cycle, DNA fragmentation and mitochondrial depolarization was evaluated.
[Mh] Termos MeSH primário: Antineoplásicos/farmacologia
Tiazóis/farmacologia
[Mh] Termos MeSH secundário: Antineoplásicos/síntese química
Antineoplásicos/química
Proliferação Celular/efeitos dos fármacos
Relação Dose-Resposta a Droga
Ensaios de Seleção de Medicamentos Antitumorais
Hemólise/efeitos dos fármacos
Seres Humanos
Estrutura Molecular
Relação Estrutura-Atividade
Tiazóis/síntese química
Tiazóis/química
Células Tumorais Cultivadas
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antineoplastic Agents); 0 (Thiazoles)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180302
[Lr] Data última revisão:
180302
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180113
[St] Status:MEDLINE


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[PMID]:27773451
[Au] Autor:Gavalas MV; Breskin A; Yuzefpolskaya M; Eisenberger A; Castagna F; Demmer RT; Flannery M; Garan AR; Takeda K; Takayama H; Naka Y; Topkara VK; Colombo PC
[Ad] Endereço:Division of Cardiology, Department of Medicine, New York Presbyterian Hospital, Columbia University, New York, New York.
[Ti] Título:Discriminatory performance of positive urine hemoglobin for detection of significant hemolysis in patients with continuous-flow left ventricular assist devices.
[So] Source:J Heart Lung Transplant;36(1):59-63, 2017 Jan.
[Is] ISSN:1557-3117
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Serum lactate dehydrogenase (LDH) is the standard measure for detection of hemolysis and thus surveillance for device thrombosis in patients on continuous-flow left ventricular assist device (CF-LVAD) support. Significant hemolysis has been defined as LDH ≥600 IU/L. However, LDH testing requires phlebotomy, precluding frequent home monitoring. Simple dipstick urinalysis (UA) for urine hemoglobin (U-Hb) overcomes this limitation. This study correlated U-Hb and LDH levels and evaluated the performance of UA for detection of significant hemolysis in patients with CF-LVADs. METHODS: U-Hb and LDH were measured concurrently 956 times in 221 patients with CF-LVADs. Statistics were computed to determine accuracy of UA in detecting LDH ≥600 IU/L, with a positive result being any detected U-Hb. All analyses were performed with and without excluding for 1) conditions associated with tissue damage, which are known to increase LDH, and 2) suspected or confirmed urinary tract infections or hematuria, which are known to cause hemoglobinuria for reasons other than hemolysis. RESULTS: Mean LDH for absent/mild/severe U-Hb was 360 IU/L/467 IU/L IU/L/777 IU/L without exclusions, 354 IU/L/444 IU/L IU/L/651 IU/L after excluding non-hemolytic LDH elevations, 370 IU/L/513 IU/L IU/L/1,357 IU/L after excluding urinary tract infections and hematuria, and 367 IU/L/470 IU/L IU/L/1,217 IU/L when both exclusions applied (all p < 0.001). Absent U-Hb had a negative predictive value for LDH ≥600 IU/L of >90% for all analyses. CONCLUSIONS: Serum LDH is significantly associated with U-Hb levels. Absence of U-Hb appears to efficiently exclude significant hemolysis in patients with CF-LVADs. Because it can be performed by patients at home, hemoglobinuria monitoring may enable more intense surveillance and earlier diagnosis of device thrombosis.
[Mh] Termos MeSH primário: Insuficiência Cardíaca/terapia
Coração Auxiliar/efeitos adversos
Hemoglobinas/metabolismo
Hemólise/fisiologia
Trombose/urina
[Mh] Termos MeSH secundário: Biomarcadores/sangue
Biomarcadores/urina
Falha de Equipamento
Feminino
Insuficiência Cardíaca/mortalidade
Insuficiência Cardíaca/urina
Seres Humanos
Incidência
Estimativa de Kaplan-Meier
L-Lactato Desidrogenase/sangue
Masculino
Meia-Idade
New York/epidemiologia
Reprodutibilidade dos Testes
Estudos Retrospectivos
Fatores de Risco
Taxa de Sobrevida/tendências
Trombose/diagnóstico
Trombose/etiologia
Urinálise
Urofolitropina
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biomarkers); 0 (Hemoglobins); 0 (Urofollitropin); EC 1.1.1.27 (L-Lactate Dehydrogenase)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180301
[Lr] Data última revisão:
180301
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161025
[St] Status:MEDLINE


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[PMID]:29345907
[Au] Autor:Alvarez-Sala A; López-García G; Attanzio A; Tesoriere L; Cilla A; Barberá R; Alegría A
[Ad] Endereço:Nutrition and Food Science Area, Faculty of Pharmacy, University of Valencia , Avda. Vicente Andrés Estellés s/n, Burjassot, Valencia 46100, Spain.
[Ti] Título:Effects of Plant Sterols or ß-Cryptoxanthin at Physiological Serum Concentrations on Suicidal Erythrocyte Death.
[So] Source:J Agric Food Chem;66(5):1157-1166, 2018 Feb 07.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The eryptotic and hemolytic effects of a phytosterol (PS) mixture (ß-sitosterol, campesterol, stigmasterol) or ß-cryptoxanthin (ß-Cx) at physiological serum concentration and their effect against oxidative stress induced by tert-butylhydroperoxide (tBOOH) (75 and 300 µM) were evaluated. ß-Cryptoxanthin produced an increase in eryptotic cells, cell volume, hemolysis, and glutathione depletion (GSH) without ROS overproduction and intracellular Ca influx. Co-incubation of both bioactive compounds protected against ß-Cx-induced eryptosis. Under tBOOH stress, PS prevented eryptosis, reducing Ca influx, ROS overproduction and GSH depletion at 75 µM, and hemolysis at both tBOOH concentrations. ß-Cryptoxanthin showed no cytoprotective effect. Co-incubation with both bioactive compounds completely prevented hemolysis and partially prevented eryptosis as well as GSH depletion induced by ß-Cx plus tBOOH. Phytosterols at physiological serum concentrations help to prevent pro-eryptotic and hemolytic effects and are promising candidate compounds for ameliorating eryptosis-associated diseases.
[Mh] Termos MeSH primário: beta-Criptoxantina/farmacologia
Eriptose/efeitos dos fármacos
Fitosteróis/farmacologia
[Mh] Termos MeSH secundário: beta-Criptoxantina/sangue
Células Cultivadas
Colesterol/análogos & derivados
Colesterol/farmacologia
Eritrócitos/química
Eritrócitos/efeitos dos fármacos
Glutationa/sangue
Hemólise/efeitos dos fármacos
Seres Humanos
Estresse Oxidativo/efeitos dos fármacos
Sitosteroides/farmacologia
Estigmasterol/farmacologia
terc-Butil Hidroperóxido/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Beta-Cryptoxanthin); 0 (Phytosterols); 0 (Sitosterols); 5L5O665639 (campesterol); 5LI01C78DD (gamma-sitosterol); 955VYL842B (tert-Butylhydroperoxide); 97C5T2UQ7J (Cholesterol); 99WUK5D0Y8 (Stigmasterol); GAN16C9B8O (Glutathione)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180226
[Lr] Data última revisão:
180226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180119
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jafc.7b05575


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[PMID]:29364903
[Au] Autor:Sala A; Cabassi CS; Santospirito D; Polverini E; Flisi S; Cavirani S; Taddei S
[Ad] Endereço:Department of Veterinary Science, University of Parma, Parma, Italy.
[Ti] Título:Novel Naja atra cardiotoxin 1 (CTX-1) derived antimicrobial peptides with broad spectrum activity.
[So] Source:PLoS One;13(1):e0190778, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Naja atra subsp. atra cardiotoxin 1 (CTX-1), produced by Chinese cobra snakes, belonging to Elapidae family, is included in the three-finger toxin family and exerts high cytotoxicity and antimicrobial activity too. Using as template mainly the tip and the subsequent ß-strand of the first "finger" of this toxin, different sequences of 20 amino acids linear peptides have been designed in order to avoid toxic effects but to maintain or even strengthen the partial antimicrobial activity already seen for the complete toxin. As a result, the sequence NCP-0 (Naja Cardiotoxin Peptide-0) was designed as ancestor and subsequently 4 other variant sequences of NCP-0 were developed. These synthesized variant sequences have shown microbicidal activity towards a panel of reference and field strains of Gram-positive and Gram-negative bacteria. The sequence named NCP-3, and its variants NCP-3a and NCP-3b, have shown the best antimicrobial activity, together with low cytotoxicity against eukaryotic cells and low hemolytic activity. Bactericidal activity has been demonstrated by minimum bactericidal concentration (MBC) assay at values below 10 µg/ml for most of the tested bacterial strains. This potent antimicrobial activity was confirmed even for unicellular fungi Candida albicans, Candida glabrata and Malassezia pachydermatis (MBC 50-6.3 µg/ml), and against the fast-growing mycobacteria Mycobacterium smegmatis and Mycobacterium fortuitum. Moreover, NCP-3 has shown virucidal activity on Bovine Herpesvirus 1 (BoHV1) belonging to Herpesviridae family. The bactericidal activity is maintained even in a high salt concentration medium (125 and 250 mM NaCl) and phosphate buffer with 20% Mueller Hinton (MH) medium against E. coli, methicillin resistant Staphylococcus aureus (MRSA) and Pseudomonas aeruginosa reference strains. Considering these in vitro obtained data, the search for active sequences within proteins presenting an intrinsic microbicidal activity could provide a new way for discovering a large number of novel and promising antimicrobial peptides families.
[Mh] Termos MeSH primário: Anti-Infecciosos/farmacologia
Proteínas Cardiotóxicas de Elapídeos/química
Peptídeos/farmacologia
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Anti-Infecciosos/química
Candida/efeitos dos fármacos
Bovinos
Dicroísmo Circular
Hemólise/efeitos dos fármacos
Herpesvirus Bovino 1/efeitos dos fármacos
Malassezia/efeitos dos fármacos
Testes de Sensibilidade Microbiana
Mycobacterium/efeitos dos fármacos
Naja naja
Peptídeos/química
Conformação Proteica
Ovinos
Staphylococcus aureus/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Infective Agents); 0 (Cobra Cardiotoxin Proteins); 0 (Peptides)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180221
[Lr] Data última revisão:
180221
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180125
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0190778


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[PMID]:29197624
[Au] Autor:Fernández ML; Quartino PY; Arce-Bejarano R; Fernández J; Camacho LF; Gutiérrez JM; Kuemmel D; Fidelio G; Lomonte B
[Ad] Endereço:Instituto Clodomiro Picado, Facultad de Microbiología, Universidad de Costa Rica San José 11501, Costa Rica.
[Ti] Título:Intravascular hemolysis induced by phospholipases A from the venom of the Eastern coral snake, Micrurus fulvius: Functional profiles of hemolytic and non-hemolytic isoforms.
[So] Source:Toxicol Lett;286:39-47, 2018 Apr.
[Is] ISSN:1879-3169
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:A unique feature of the venom of Micrurus fulvius (Eastern coral snake) is its ability to induce severe intravascular hemolysis in particular species, such as dogs or mice. This effect was previously shown to be induced by distinct phospholipase A (PLA ) isoforms which cause direct hemolysis in vitro, an uncommon finding for such enzymes. The functional profiles of PLA -17, a direct hemolytic enzyme, and PLA -12, a co-existing venom isoform lacking such effect, were compared. The enzymes differed not only in their ability to cause intravascular hemolysis: PLA -17 additionally displayed lethal, myotoxic, and anticoagulant actions, whereas PLA -12 lacked these effects. PLA -12 was much more active in hydrolyzing a monodisperse synthetic substrate than PLA -17, but the catalytic activity of latter was notably higher on a micellar substrate, or towards pure phospholipid artificial monolayers under controlled lateral pressures. Interestingly, PLA -17 could hydrolyze substrate at a pressure of 20 mN m , in contrast to PLA -12 or the non-toxic pancreatic PLA . This suggests important differences in the monolayer penetrating power, which could be related to differences in toxicity. Comparative examination of primary structures and predicted three-dimensional folding of PLA -12 and PLA -17, revealed that differences concentrate in their N-terminal and central regions, leading to variations of the surface properties at the membrane interacting interface. PLA -17 presents a less basic interfacial surface than PLA -12, but more bulky aromatic residues, which could be associated to its higher membrane-penetrating strength. Altogether, these structural and functional comparative observations suggest that the ability of PLA s to penetrate substrate interfaces could be a major determinant of toxicity, perhaps more important than protein surface charge.
[Mh] Termos MeSH primário: Cobras Corais
Venenos Elapídicos/toxicidade
Hemólise/efeitos dos fármacos
Fosfolipases A2/toxicidade
Proteínas de Répteis/toxicidade
[Mh] Termos MeSH secundário: Animais
Relação Dose-Resposta a Droga
Venenos Elapídicos/enzimologia
Feminino
Masculino
Camundongos
Modelos Moleculares
Permeabilidade
Fosfolipases A2/química
Fosfolipases A2/metabolismo
Conformação Proteica
Dobramento de Proteína
Isoformas de Proteínas
Proteínas de Répteis/química
Proteínas de Répteis/metabolismo
Relação Estrutura-Atividade
Propriedades de Superfície
Fatores de Tempo
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Elapid Venoms); 0 (Protein Isoforms); 0 (Reptilian Proteins); 0 (micrurus venom); EC 3.1.1.4 (Phospholipases A2)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180220
[Lr] Data última revisão:
180220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171204
[St] Status:MEDLINE


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[PMID]:29287997
[Au] Autor:Machado KDC; Sousa LQ; Lima DJB; Soares BM; Cavalcanti BC; Maranhão SS; Noronha JDC; Rodrigues DJ; Militão GCG; Chaves MH; Vieira-Júnior GM; Pessoa C; Moraes MO; Sousa JMCE; Melo-Cavalcante AAC; Ferreira PMP
[Ad] Endereço:Postgraduate Program in Pharmaceutical Sciences, Federal University of Piauí, Teresina, Brazil.
[Ti] Título:Marinobufagin, a molecule from poisonous frogs, causes biochemical, morphological and cell cycle changes in human neoplasms and vegetal cells.
[So] Source:Toxicol Lett;285:121-131, 2018 Mar 15.
[Is] ISSN:1879-3169
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Skin toad secretion present physiologically active molecules to protect them against microorganisms, predators and infections. This work detailed the antiproliferative action of marinobufagin on tumor and normal lines, investigate its mechanism on HL-60 leukemia cells and its toxic effects on Allium cepa meristematic cells. Initially, cytotoxic action was assessed by colorimetric assays. Next, HL-60 cells were analyzed by morphological and flow cytometry techniques and growing A. cepa roots were examined after 72 h exposure. Marinobufagin presented high antiproliferative action against all human tumor lines [IC values ranging from 0.15 (leukemia) to 7.35 (larynx) µM] and it failed against human erythrocytes and murine lines. Human normal peripheral blood mononuclear cells (PBMC) were up to 72.5-fold less sensitive [IC 10.88 µM] to marinobufagin than HL-60 line, but DNA strand breaks were no detected. Leukemia treaded cells exhibited cell viability reduction, DNA fragmentation, phosphatidylserine externalization, binucleation, nuclear condensation and cytoplasmic vacuoles. Marinobufagin also reduced the growth of A. cepa roots (EC : 7.5 µM) and mitotic index, caused cell cycle arrest and chromosomal alterations (micronuclei, delays and C-metaphases) in meristematic cells. So, to find out partially targeted natural molecules on human leukemia cells, like marinobufagin, is an amazing and stimulating way to continue the battle against cancer.
[Mh] Termos MeSH primário: Antineoplásicos/farmacologia
Bufanolídeos/farmacologia
Ciclo Celular/efeitos dos fármacos
Quebras de DNA
Cebolas/efeitos dos fármacos
[Mh] Termos MeSH secundário: Adolescente
Adulto
Animais
Antineoplásicos/isolamento & purificação
Antineoplásicos/toxicidade
Bufanolídeos/isolamento & purificação
Bufanolídeos/toxicidade
Bufonidae/metabolismo
Sobrevivência Celular/efeitos dos fármacos
Ensaio Cometa
Relação Dose-Resposta a Droga
Eritrócitos/efeitos dos fármacos
Células HL-60
Voluntários Saudáveis
Hemólise/efeitos dos fármacos
Seres Humanos
Leucócitos Mononucleares/efeitos dos fármacos
Meristema/citologia
Meristema/efeitos dos fármacos
Meristema/genética
Micronúcleos com Defeito Cromossômico/induzido quimicamente
Cebolas/citologia
Cebolas/genética
Pele/secreção
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antineoplastic Agents); 0 (Bufanolides); 3KBT25GV2B (marinobufagenin)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180219
[Lr] Data última revisão:
180219
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171231
[St] Status:MEDLINE


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[PMID]:29309785
[Au] Autor:Pathak V; Colah R; Ghosh K
[Ad] Endereço:Department of Haematogenetics, National Institute of Immunohaematology (ICMR), KEM Hospital, Parel 400012, Mumbai, India.
[Ti] Título:Plasmodium falciparum malaria skews globin gene expression balance in in-vitro haematopoietic stem cell culture system: Its implications in malaria associated anemia.
[So] Source:Exp Parasitol;185:29-38, 2018 Feb.
[Is] ISSN:1090-2449
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Understanding the pathophysiology and associated host parasite interactions of the malaria infection is the prerequisite for developing effective prevention and treatment strategies. The exact mechanism underlying malaria associated ineffective and dyserythropoiesis is not yet fully understood. Being an important protein, haemoglobin serves as the main amino acid reservoir available to the intra-erythrocytic plasmodium. It is important to check the expression profiling of globin genes which may help us to understand host parasite interactions and its potential contribution to both infection and disease. Here, an in-vitro culture system was used to study the effect of different doses of Plasmodium falciparum on haematopoietic stem cell expansion, differentiation and expression of globin genes. Upon exposure to the different doses of P. falciparum parasites of strains 3D7, Dd2 and RKL9 (intact and lysed form) at different stages of erythroid development, cells demonstrated suppression in growth and differentiation. At almost all stages of erythroid development upon parasite exposure, the γ globin gene was found to be downregulated and the α/ß as well as α/non- α globin mRNA ratios in late stage erythroid cells were found to be reduced (p < .01) compared to the untreated controls. The imbalance in globin chain expression might be considered as one of the factors involved in malaria associated inappropriate erythropoietic responses.
[Mh] Termos MeSH primário: Anemia/etiologia
Regulação da Expressão Gênica/genética
Globinas/genética
Células-Tronco Hematopoéticas/parasitologia
Malária Falciparum/genética
[Mh] Termos MeSH secundário: Anemia/genética
Anemia/metabolismo
Antígenos CD34/sangue
Biomarcadores/metabolismo
Células Cultivadas
Eritrócitos/parasitologia
Eritrócitos/patologia
Células Eritroides/imunologia
Sangue Fetal/citologia
Globinas/metabolismo
Células-Tronco Hematopoéticas/metabolismo
Hemólise
Interações Hospedeiro-Parasita/genética
Seres Humanos
Malária Falciparum/complicações
Reação em Cadeia da Polimerase em Tempo Real
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antigens, CD34); 0 (Biomarkers); 9004-22-2 (Globins)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180214
[Lr] Data última revisão:
180214
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180109
[St] Status:MEDLINE



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