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Pesquisa : D01.029.260.700.675.374.075.025.300.150 [Categoria DeCS]
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  1 / 13113 MEDLINE  
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[PMID]:29238189
[Au] Autor:Zhang CY; Sun XY; Ouyang JM; Gui BS
[Ad] Endereço:Institute of Biomineralization and Lithiasis Research, Jinan University, Guangzhou.
[Ti] Título:Diethyl citrate and sodium citrate reduce the cytotoxic effects of nanosized hydroxyapatite crystals on mouse vascular smooth muscle cells.
[So] Source:Int J Nanomedicine;12:8511-8525, 2017.
[Is] ISSN:1178-2013
[Cp] País de publicação:New Zealand
[La] Idioma:eng
[Ab] Resumo:Objective: This study aimed to investigate the damage mechanism of nanosized hydroxyapatite (nano-HAp) on mouse aortic smooth muscle cells (MOVASs) and the injury-inhibiting effects of diethyl citrate (Et Cit) and sodium citrate (Na Cit) to develop new drugs that can simultaneously induce anticoagulation and inhibit vascular calcification. Methods: The change in cell viability was evaluated using a cell proliferation assay kit, and the amount of lactate dehydrogenase (LDH) released was measured using an LDH kit. Intracellular reactive oxygen species (ROS) and mitochondrial damage were detected by DCFH-DA staining and JC-1 staining. Cell apoptosis and necrosis were detected by Annexin V staining. Intracellular calcium concentration and lysosomal integrity were measured using Fluo-4/AM and acridine orange, respectively. Results: Nano-HAp decreased cell viability and damaged the cell membrane, resulting in the release of a large amount of LDH. Nano-HAp entered the cells and damaged the mitochondria, and then induced cell apoptosis by producing a large amount of ROS. In addition, nano-HAp increased the intracellular Ca concentration, leading to lysosomal rupture and cell necrosis. On addition of the anticoagulant Et Cit or Na Cit, cell viability and mitochondrial membrane potential increased, whereas the amount of LDH released, ROS, and apoptosis rate decreased. Et Cit and Na Cit could also chelate with Ca to inhibit the intracellular Ca elevations induced by nano-HAp, prevent lysosomal rupture, and reduce cell necrosis. High concentrations of Et Cit and Na Cit exhibited strong inhibitory effects. The inhibitory capacity of Na Cit was stronger than that of Et Cit at similar concentrations. Conclusion: Both Et Cit and Na Cit significantly reduced the cytotoxicity of nano-HAp on MOVASs and inhibited the apoptosis and necrosis induced by nano-HAp crystals. The chelating function of citrate resulted in both anticoagulation and binding to HAp. Et Cit and Na Cit may play a role as anticoagulants in reducing injury to the vascular wall caused by nano-HAp.
[Mh] Termos MeSH primário: Citratos/farmacologia
Durapatita/efeitos adversos
Músculo Liso Vascular/citologia
Nanopartículas/efeitos adversos
[Mh] Termos MeSH secundário: Animais
Anticoagulantes/farmacologia
Apoptose/efeitos dos fármacos
Calcinose/prevenção & controle
Cálcio/metabolismo
Sobrevivência Celular/efeitos dos fármacos
Células Cultivadas
Durapatita/química
Potencial da Membrana Mitocondrial/efeitos dos fármacos
Camundongos
Mitocôndrias/efeitos dos fármacos
Mitocôndrias/metabolismo
Músculo Liso Vascular/efeitos dos fármacos
Músculo Liso Vascular/metabolismo
Nanopartículas/química
Espécies Reativas de Oxigênio/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anticoagulants); 0 (Citrates); 0 (Reactive Oxygen Species); 0 (diethyl citrate); 1Q73Q2JULR (sodium citrate); 91D9GV0Z28 (Durapatite); SY7Q814VUP (Calcium)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171215
[St] Status:MEDLINE
[do] DOI:10.2147/IJN.S145386


  2 / 13113 MEDLINE  
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[PMID]:29200851
[Au] Autor:Mondal S; Manivasagan P; Bharathiraja S; Santha Moorthy M; Kim HH; Seo H; Lee KD; Oh J
[Ad] Endereço:Marine-Integrated Bionics Research Center.
[Ti] Título:Magnetic hydroxyapatite: a promising multifunctional platform for nanomedicine application.
[So] Source:Int J Nanomedicine;12:8389-8410, 2017.
[Is] ISSN:1178-2013
[Cp] País de publicação:New Zealand
[La] Idioma:eng
[Ab] Resumo:In this review, specific attention is paid to the development of nanostructured magnetic hydroxyapatite (MHAp) and its potential application in controlled drug/gene delivery, tissue engineering, magnetic hyperthermia treatment, and the development of contrast agents for magnetic resonance imaging. Both magnetite and hydroxyapatite materials have excellent prospects in nanomedicine with multifunctional therapeutic approaches. To date, many research articles have focused on biomedical applications of nanomaterials because of which it is very difficult to focus on any particular type of nanomaterial. This study is possibly the first effort to emphasize on the comprehensive assessment of MHAp nanostructures for biomedical applications supported with very recent experimental studies. From basic concepts to the real-life applications, the relevant characteristics of magnetic biomaterials are patented which are briefly discussed. The potential therapeutic and diagnostic ability of MHAp-nanostructured materials make them an ideal platform for future nanomedicine. We hope that this advanced review will provide a better understanding of MHAp and its important features to utilize it as a promising material for multifunctional biomedical applications.
[Mh] Termos MeSH primário: Durapatita/química
Magnetismo
Nanomedicina/métodos
[Mh] Termos MeSH secundário: Animais
Sistemas de Liberação de Medicamentos
Seres Humanos
Hipertermia Induzida
Nanopartículas de Magnetita/química
Tecidos Suporte/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Magnetite Nanoparticles); 91D9GV0Z28 (Durapatite)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171205
[St] Status:MEDLINE
[do] DOI:10.2147/IJN.S147355


  3 / 13113 MEDLINE  
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[PMID]:28456893
[Au] Autor:Kanazawa M; Tsuru K; Fukuda N; Sakemi Y; Nakashima Y; Ishikawa K
[Ad] Endereço:Department of Orthopaedic Surgery, Graduate School of Medical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka, 812-8582, Japan.
[Ti] Título:Evaluation of carbonate apatite blocks fabricated from dicalcium phosphate dihydrate blocks for reconstruction of rabbit femoral and tibial defects.
[So] Source:J Mater Sci Mater Med;28(6):85, 2017 Jun.
[Is] ISSN:1573-4838
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:This study aimed to evaluate in vivo behavior of a carbonate apatite (CO Ap) block fabricated by compositional transformation via a dissolution-precipitation reaction using a calcium hydrogen phosphate dihydrate [DCPD: CaHPO ·2H O] block as a precursor. These blocks were used to reconstruct defects in the femur and tibia of rabbits, using sintered dense hydroxyapatite (HAp) blocks as the control. Both the CO Ap and HAp blocks showed excellent tissue response and good osteoconductivity. HAp block maintained its structure even after 24 weeks of implantation, so no bone replacement of the implant was observed throughout the post-implantation period in either femoral or tibial bone defects. In contrast, CO Ap was resorbed with increasing time after implantation and replaced with new bone. The CO Ap block was resorbed approximately twice as fast at the metaphysis of the proximal tibia than at the epiphysis of the distal femur. The CO Ap block was resorbed at an approximately linear change over time, with complete resorption was estimated by extrapolation of data at approximately 1-1.5 years. Hence, the CO Ap block fabricated in this study has potential value as an ideal artificial bone substitute because of its resorption and subsequent replacement by bone.
[Mh] Termos MeSH primário: Apatitas/química
Substitutos Ósseos
Fosfatos de Cálcio/química
[Mh] Termos MeSH secundário: Animais
Durapatita
Epífises
Fêmur
Próteses e Implantes
Coelhos
Tíbia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Apatites); 0 (Bone Substitutes); 0 (Calcium Phosphates); 55326-60-8 (carboapatite); 91D9GV0Z28 (Durapatite); O7TSZ97GEP (calcium phosphate, dibasic, dihydrate)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170501
[St] Status:MEDLINE
[do] DOI:10.1007/s10856-017-5896-5


  4 / 13113 MEDLINE  
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[PMID]:28464466
[Au] Autor:Jiang Z; Zhang Z; Wu T; Zhang P; Song J; Xie C; Han B
[Ad] Endereço:Beijing National Laboratory for Molecular Sciences, CAS Key Laboratory of Colloid and Interface and Chemical Thermodynamics, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190, China.
[Ti] Título:Efficient Generation of Lactic Acid from Glycerol over a Ru-Zn-Cu /Hydroxyapatite Catalyst.
[So] Source:Chem Asian J;12(13):1598-1604, 2017 Jul 04.
[Is] ISSN:1861-471X
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:The biodiesel production process generates a significant amount of glycerol as a byproduct. A drive to add value has attracted worldwide attention, with the aim of improving the overall effectiveness and profitability of biodiesel production. Herein, we report hydroxyapatite (HAP)-supported Ru-Zn-Cu (Ru-Zn-Cu /HAP) as effective catalysts for the transformation of glycerol to lactic acid (LA).The catalysts were characterized by using different techniques. The effects of catalyst composition, reaction time, and temperature on the conversion and product distribution were investigated. It was revealed that Ru nanoparticles of less than 2 nm were dispersed uniformly on HAP. Cu could effectively inhibit the cleavage of C-C bonds, which led to improved yields of LA. Under optimized conditions, the yield of LA could reach 70.9 % over Ru-Zn-Cu /HAP. Furthermore, the catalyst could be reused at least four times without an obvious loss of activity or selectivity.
[Mh] Termos MeSH primário: Cobre/química
Durapatita/química
Glicerol/química
Ácido Láctico/síntese química
Rutênio/química
Zinco/química
[Mh] Termos MeSH secundário: Catálise
Ácido Láctico/química
Tamanho da Partícula
Propriedades de Superfície
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
33X04XA5AT (Lactic Acid); 789U1901C5 (Copper); 7UI0TKC3U5 (Ruthenium); 91D9GV0Z28 (Durapatite); J41CSQ7QDS (Zinc); PDC6A3C0OX (Glycerol)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180222
[Lr] Data última revisão:
180222
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170503
[St] Status:MEDLINE
[do] DOI:10.1002/asia.201700412


  5 / 13113 MEDLINE  
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[PMID]:29229389
[Au] Autor:Perez VA; Mangum JE; Hubbard MJ
[Ad] Endereço:Department of Pharmacology & Therapeutics, The University of Melbourne, Victoria, Australia; Department of Pediatric Stomatology, University of Talca, Talca, Chile.
[Ti] Título:Direct evidence that KLK4 is a hydroxyapatite-binding protein.
[So] Source:Biochem Biophys Res Commun;495(2):1896-1900, 2018 01 08.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The protease kallikrein 4 (KLK4) plays a pivotal role during dental enamel formation by degrading the major enamel protein, amelogenin, prior to the final steps of enamel hardening. KLK4 dysfunction is known to cause some types of developmental defect in enamel but the mechanisms responsible for transient retention of KLK4 in semi-hardened enamel matrix remain unclear. To address contradictory reports about the affinity of KLK4 for enamel hydroxyapatite-like mineral, we used pure components in quasi-physiological conditions and found that KLK4 binds hydroxyapatite directly. Hypothesising KLK4 self-destructs once amelogenin is degraded, biochemical analyses revealed that KLK4 progressively lost activity, became aggregated, and autofragmented when incubated without substrate in both the presence and absence of reducer. However, with non-ionic detergent present as proxy substrate, KLK4 remained active and intact throughout. These findings prompt a new mechanistic model and line of enquiry into the role of KLK4 in enamel hardening and malformation.
[Mh] Termos MeSH primário: Esmalte Dentário/química
Esmalte Dentário/ultraestrutura
Durapatita/química
Calicreínas/química
Calicreínas/ultraestrutura
[Mh] Termos MeSH secundário: Sítios de Ligação
Ativação Enzimática
Estabilidade Enzimática
Ligação Proteica
Especificidade por Substrato
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
91D9GV0Z28 (Durapatite); EC 3.4.21.- (Kallikreins); EC 3.4.21.- (kallikrein 4)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180214
[Lr] Data última revisão:
180214
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171213
[St] Status:MEDLINE


  6 / 13113 MEDLINE  
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[PMID]:29025652
[Au] Autor:Lu Y; Li M; Li L; Wei S; Hu X; Wang X; Shan G; Zhang Y; Xia H; Yin Q
[Ad] Endereço:Southern Medical University, No. 1023, South Shatai Road, Baiyun District, Guangzhou, Guangdong 510515, China; Guangdong Key Laboratory of Orthopaedic Technology and Implant Materials, Key Laboratory of Trauma and Tissue Repair of Tropical Area of PLA, Department of Orthopedics, Guangzhou General Ho
[Ti] Título:High-activity chitosan/nano hydroxyapatite/zoledronic acid scaffolds for simultaneous tumor inhibition, bone repair and infection eradication.
[So] Source:Mater Sci Eng C Mater Biol Appl;82:225-233, 2018 Jan 01.
[Is] ISSN:1873-0191
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Implanted biomaterials combined tumor inhibition and bone repair property are urgently needed to address the huge bone destruction and the high local recurrence following primary surgery in bone tumor therapy. In this work, a high-activity chitosan/nano hydroxyapatite (CS/nHA) scaffold containing zoledronic acid (CS/nHA/Zol) was prepared with a facile method. The prepared CS/nHA/Zol scaffolds exhibited excellent tumor inhibition property towards giant cell tumor of bone (GCT) in vitro through inducing cells apoptosis by up-regulating pro-apoptosis genes expression and reducing the osteoclastic activity of tumor cells by down-regulating osteoclastic genes. Meanwhile, the prepared scaffolds possessed well biocompatibility and osteoinductivity as compared to pure CS/nHA scaffolds. Furthermore, the prepared scaffolds also presented outstanding antibacterial activity against clinical pathogenic S. aureus and E. coli. These overall findings successfully demonstrated the prepared CS/nHA/Zol scaffolds had a multifunction of tumor therapy, bone repair, and antibacterium, which provides a new approach possessed promising advantages in bone tumor therapy.
[Mh] Termos MeSH primário: Materiais Biocompatíveis/química
Substitutos Ósseos/química
Quitosana/química
Difosfonatos/química
Durapatita/química
Imidazóis/química
Nanocompostos/química
[Mh] Termos MeSH secundário: Apoptose/efeitos dos fármacos
Materiais Biocompatíveis/farmacologia
Materiais Biocompatíveis/toxicidade
Regeneração Óssea/efeitos dos fármacos
Substitutos Ósseos/farmacologia
Células Cultivadas
Técnicas de Cocultura
Eritrócitos/citologia
Eritrócitos/efeitos dos fármacos
Eritrócitos/metabolismo
Escherichia coli/efeitos dos fármacos
Hemólise/efeitos dos fármacos
Seres Humanos
Células Mesenquimais Estromais/citologia
Células Mesenquimais Estromais/efeitos dos fármacos
Células Mesenquimais Estromais/metabolismo
Microscopia Confocal
Microscopia Eletrônica de Varredura
Espectroscopia de Infravermelho com Transformada de Fourier
Staphylococcus aureus/efeitos dos fármacos
Engenharia Tecidual
Tecidos Suporte/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biocompatible Materials); 0 (Bone Substitutes); 0 (Diphosphonates); 0 (Imidazoles); 6XC1PAD3KF (zoledronic acid); 9012-76-4 (Chitosan); 91D9GV0Z28 (Durapatite)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180205
[Lr] Data última revisão:
180205
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171014
[St] Status:MEDLINE


  7 / 13113 MEDLINE  
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[PMID]:28464615
[Au] Autor:Nygren H; Bigdeli N; Ilver L; Malmberg P
[Ad] Endereço:Department of Medical Chemistry and Cell Biology, Institute of Biomedicine, Sahlgrenska Academy, University of Gothenburg, POB 440, 40530 Gothenburg, Sweden.
[Ti] Título:Mg-corrosion, hydroxyapatite, and bone healing.
[So] Source:Biointerphases;12(2):02C407, 2017 05 02.
[Is] ISSN:1559-4106
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The different capacities of magnesium in the metallic form (Mg-metal) and magnesium oxide (MgO) to stimulate bone healing are possible clues in the search for products that may promote bone healing. Since both Mg-metal and MgO can be assumed to release comparable amounts of Mg ions during their reactions in the tissue where they have been implanted, it is of some importance to follow this process and analyze the resulting mineral formation in the tissue at the implantation site. Implants of MgO were inserted into rat tibia, and the bone healing was compared with sham-operated controls. Samples were taken after 1 week of healing and analyzed by histology, environmental scanning electron microscopy equipped with an energy dispersive x-ray spectroscopy analyzer, and time-of-flight secondary ion mass spectrometry (ToF-SIMS). Callus bone was seen in sham-operated controls after 1 week of healing. Implantation of MgO impaired the callus bone formation by replacing bone with apparently mineralized areas, lacking osteocytes and were denoted, amorphous bodies. Elemental analysis showed increased levels of Ca (7.1%), P (3.7%), and Mg (0.2%) in the bone marrow of MgO-treated animals versus sham-operated controls Ca (2.4%), P (2.3%), and Mg (0.1%). The Ca content of the cortical bone was also significantly increased (Ca, 29% increase) in MgO-treated animals compared to sham-operated controls. The Ca content of the cortical bone of sham-operated animals was also significantly (p < 0.05) higher than the corresponding value of untreated animals, which means that the surgical trauma induces an altered composition of the bone mineral. The Ca/P ratio was 1.26-1.68, which is compatible with that of mineralized bone with different contents of organic materials. Analysis of bone sections using ToF-SIMS showed the presence of hydroxyapatite (HA) and MgCO in the bone marrow and in cortical bone. Analysis using x-ray photoelectron spectroscopy of Mg, MgO, and MgCO after incubation with cell culture medium (DMEM), in vitro, showed binding of CaPO at the Mg and MgO samples. The Ca/P ratio was 0.8, indicating a higher P content than that expected for HA. Exposure of human embryonic stem cells to Mg species preincubated in DMEM resulted in HA production by the cells. Thus, two sources of CaPO in the bone marrow of MgO-treated bone were defined, catalytic formation on Mg-species and synthesis from activated stem-cells. The presented data suggest that bone healing near Mg implants is congruent with the fracture healing of bone, boosted by high HA levels in the bone marrow. In this context, the different capacities of Mg-metal and MgO to catalyse the formation of HA can be important clues to their different bone promoting effects.
[Mh] Termos MeSH primário: Calo Ósseo/metabolismo
Osso Cortical/lesões
Osso Cortical/metabolismo
Durapatita/farmacologia
Consolidação da Fratura/efeitos dos fármacos
Óxido de Magnésio/farmacologia
[Mh] Termos MeSH secundário: Animais
Calo Ósseo/patologia
Linhagem Celular
Corrosão
Osso Cortical/patologia
Células-Tronco Embrionárias Humanas
Seres Humanos
Magnésio/farmacologia
Masculino
Teste de Materiais
Ratos
Ratos Sprague-Dawley
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
3A3U0GI71G (Magnesium Oxide); 91D9GV0Z28 (Durapatite); I38ZP9992A (Magnesium)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180201
[Lr] Data última revisão:
180201
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170504
[St] Status:MEDLINE
[do] DOI:10.1116/1.4982601


  8 / 13113 MEDLINE  
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[PMID]:28460528
[Au] Autor:Fears KP; Love CT; Day DE
[Ad] Endereço:Chemistry Division, U.S. Naval Research Laboratory, 4555 Overlook Ave. SW, Washington, DC 20375.
[Ti] Título:Albumin conformational change and aggregation induced by nanostructured apatites.
[So] Source:Biointerphases;12(2):02D403, 2017 05 01.
[Is] ISSN:1559-4106
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Biomaterials with nanostructured surfaces influence cellular response in a significantly different, and often beneficial, manner compared to materials with coarser features. Hydroxyapatite [HA, Ca (PO ) (OH) ] and strontium-apatite [Sr (PO ) (OH) ] microspheres that present nanotopographies similar to biological apatites were incubated in albumin solutions, at physiological conditions (40 mg ml ; 37 °C), for up to 72 h. Electronic and vibrational circular dichroism spectroscopies revealed spectral signatures characteristic of stacked ß-sheet regions in higher ordered structures (e.g., fibrils). The presence of stacked ß-sheets was further evidenced by thioflavin T staining. The sequestration of interfacial Ca atoms by pyrophosphate ions (P O ), prior to albumin adsorption, prevented stacked ß-sheet formation on hydroxyapatite. These results suggest that the charge and/or spatial arrangement of Ca atoms direct stacked ß-sheet formation during bovine serum albumin adsorption. Stacked ß-sheet spectral features were also observed after incubating HA in fetal bovine serum, highlighting that this phenomena could direct cellular response to these biomaterials in vivo.
[Mh] Termos MeSH primário: Durapatita/química
Nanoestruturas/química
Soroalbumina Bovina/química
[Mh] Termos MeSH secundário: Animais
Bovinos
Estrutura Secundária de Proteína
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Nm] Nome de substância:
27432CM55Q (Serum Albumin, Bovine); 91D9GV0Z28 (Durapatite)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180201
[Lr] Data última revisão:
180201
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170503
[St] Status:MEDLINE
[do] DOI:10.1116/1.4982641


  9 / 13113 MEDLINE  
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[PMID]:28743379
[Au] Autor:Schumacher TC; Tushtev K; Wagner U; Becker C; Große Holthaus M; Hein SB; Haack J; Heiss C; Engelhardt M; El Khassawna T; Rezwan K
[Ad] Endereço:Advanced Ceramics, University of Bremen, 28359 Bremen, Germany.
[Ti] Título:A novel, hydroxyapatite-based screw-like device for anterior cruciate ligament (ACL) reconstructions.
[So] Source:Knee;24(5):933-939, 2017 Oct.
[Is] ISSN:1873-5800
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Rupture of the anterior cruciate ligament (ACL) is one of the most common injuries of the knee. Common techniques for ACL reconstruction require a graft fixation using interference screws. Nowadays, these interference screws are normally made of titanium or polymer/ceramic composites. The main challenge of application of a fixation device made entirely of bioactive ceramic is in relation to the low strength of such materials. The purpose of this study was to evaluate a novel geometry for a fixation device made of pure hydroxyapatite for ACL reconstructions that can overcome some problems of the titanium and the polymer/ceramic screws. METHODS: Finite Element Analysis was used for optimization of the stress distribution in conventional interference screw geometry. For experimental evaluation of the new fixation device, ex vivo tests were performed. RESULTS: The innovative screw-like fixation device is characterized by multiple threads with a large thread pitch. The novel design enabled the insertion of the screw into the bone without the application of an external torque or a screwdriver. In turn, it also allowed for the use of low-strength and high-bioactivity materials, like hydroxyapatite. Ex vivo tests showed that the novel screw can sustain pull-out forces up to 476 N, which is comparable to that of the commercially available BioComposite™ interference screws (Arthrex Inc., Germany), as a reference. CONCLUSIONS: In summary, the novel screw design is a promising strategy to develop all-ceramic fixation devices for ACL reconstructions, which may eliminate some drawbacks of the current interference screws.
[Mh] Termos MeSH primário: Implantes Absorvíveis
Parafusos Ósseos
Durapatita
[Mh] Termos MeSH secundário: Animais
Articulação do Tornozelo/cirurgia
Simulação por Computador
Projeto Auxiliado por Computador
Análise de Elementos Finitos
Modelos Anatômicos
Modelos Animais
Desenho de Prótese
Ovinos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
91D9GV0Z28 (Durapatite)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180110
[Lr] Data última revisão:
180110
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170727
[St] Status:MEDLINE


  10 / 13113 MEDLINE  
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[PMID]:29186202
[Au] Autor:Fu C; Bai H; Zhu J; Niu Z; Wang Y; Li J; Yang X; Bai Y
[Ad] Endereço:Department of Orthopedic Surgery, the Second Hospital of Jilin University, Changchun, Jilin, P. R. China.
[Ti] Título:Enhanced cell proliferation and osteogenic differentiation in electrospun PLGA/hydroxyapatite nanofibre scaffolds incorporated with graphene oxide.
[So] Source:PLoS One;12(11):e0188352, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:One of the goals of bone tissue engineering is to mimic native ECM in architecture and function, creating scaffolds with excellent biocompatibility, osteoinductive ability and mechanical properties. The aim of this study was to fabricate nanofibrous matrices by electrospinning a blend of poly (L-lactic-co-glycolic acid) (PLGA), hydroxyapatite (HA), and grapheme oxide (GO) as a favourable platform for bone tissue engineering. The morphology, biocompatibility, mechanical properties, and biological activity of all nanofibrous matrices were compared. The data indicate that the hydrophilicity and protein adsorption rate of the fabricated matrices were significantly increased by blending with a small amount of HA and GO. Furthermore, GO significantly boosted the tensile strength of the nanofibrous matrices, and the PLGA/GO/HA nanofibrous matrices can serve as mechanically stable scaffolds for cell growth. For further test in vitro, MC3T3-E1 cells were cultured on the PLGA/HA/GO nanofbrous matrices to observe various cellular activities and cell mineralization. The results indicated that the PLGA/GO/HA nanofibrous matrices significantly enhanced adhesion, and proliferation in MCET3-E1 cells and functionally promoted alkaline phosphatase (ALP) activity, the osteogenesis-related gene expression and mineral deposition. Therefore, the PLGA/HA/GO composite nanofibres are excellent and versatile scaffolds for applications in bone tissue regeneration.
[Mh] Termos MeSH primário: Diferenciação Celular
Proliferação Celular
Durapatita
Grafite/química
Ácido Láctico
Nanofibras
Osteogênese/fisiologia
Ácido Poliglicólico
Tecidos Suporte
[Mh] Termos MeSH secundário: Células 3T3
Adsorção
Animais
Camundongos
Resistência à Tração
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (polylactic acid-polyglycolic acid copolymer); 26009-03-0 (Polyglycolic Acid); 33X04XA5AT (Lactic Acid); 7782-42-5 (Graphite); 91D9GV0Z28 (Durapatite)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171226
[Lr] Data última revisão:
171226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171130
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0188352



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