Base de dados : MEDLINE
Pesquisa : D01.029.260.877.700 [Categoria DeCS]
Referências encontradas : 969 [refinar]
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[PMID]:28809078
[Au] Autor:Kuo YH; Konopko AM; Borotto NB; Majmudar JD; Haynes SE; Martin BR
[Ad] Endereço:Program in Chemical Biology, Department of Chemistry, University of Michigan, Ann Arbor, MI, 48109, USA.
[Ti] Título:Profiling Protein S-Sulfination with Maleimide-Linked Probes.
[So] Source:Chembiochem;18(20):2028-2032, 2017 Oct 18.
[Is] ISSN:1439-7633
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Cysteine residues are susceptible to oxidation to form S-sulfinyl (R-SO H) and S-sulfonyl (R-SO H) post-translational modifications. Here we present a simple bioconjugation strategy to label S-sulfinated proteins by using reporter-linked maleimides. After alkylation of free thiols with iodoacetamide, S-sulfinated cysteines react with maleimide to form a sulfone Michael adduct that remains stable under acidic conditions. Using this sequential alkylation strategy, we demonstrate differential S-sulfination across mouse tissue homogenates, as well as enhanced S-sulfination following pharmacological induction of endoplasmic reticulum stress, lipopolysaccharide stimulation, and inhibitors of the electron transport chain. Overall, this study reveals a broadened profile of maleimide reactivity across cysteine modifications, and outlines a simple method for profiling the physiological role of cysteine S-sulfination in disease.
[Mh] Termos MeSH primário: Maleimidas/química
Sondas Moleculares/química
Proteínas/química
Proteínas/metabolismo
Ácidos Sulfínicos/metabolismo
Enxofre/metabolismo
[Mh] Termos MeSH secundário: Células HEK293
Seres Humanos
Modelos Moleculares
Conformação Proteica
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Maleimides); 0 (Molecular Probes); 0 (Proteins); 0 (Sulfinic Acids); 2519R1UGP8 (maleimide); 70FD1KFU70 (Sulfur)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171030
[Lr] Data última revisão:
171030
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170816
[St] Status:MEDLINE
[do] DOI:10.1002/cbic.201700137


  2 / 969 MEDLINE  
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[PMID]:28734665
[Au] Autor:Herrero Alvarez N; van de Langemheen H; Brouwer AJ; Liskamp RMJ
[Ad] Endereço:School of Chemistry, Joseph Black Building, University of Glasgow, University Avenue, Glasgow G12 8QQ, UK.
[Ti] Título:Potential peptidic proteasome inhibitors by incorporation of an electrophilic trap based on amino acid derived α-substituted sulfonyl fluorides.
[So] Source:Bioorg Med Chem;25(19):5055-5063, 2017 Oct 01.
[Is] ISSN:1464-3391
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Peptido sulfonyl fluoride derivatives were designed and synthesized containing a substituent on the alpha position (αPSFs) with respect to the sulfonyl fluoride electrophilic trap. The chemical reactivity of these α-substituted amino sulfonyl fluorides was studied and compared with the previously described ß-substituted amino sulfonyl fluorides in order to get a deeper insight into the importance of the immediate structural environment of the sulfonyl fluoride moiety. Unfortunately, the poor solubility of the resulting αPSFs precluded a proper evaluation of their biological activity.
[Mh] Termos MeSH primário: Desenho de Drogas
Peptidomiméticos/química
Peptidomiméticos/farmacologia
Inibidores de Proteassoma/química
Inibidores de Proteassoma/farmacologia
Ácidos Sulfínicos/química
Ácidos Sulfínicos/farmacologia
[Mh] Termos MeSH secundário: Aminoácidos/síntese química
Aminoácidos/química
Aminoácidos/farmacologia
Seres Humanos
Peptidomiméticos/síntese química
Inibidores de Proteassoma/síntese química
Solubilidade
Ácidos Sulfínicos/síntese química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Amino Acids); 0 (Peptidomimetics); 0 (Proteasome Inhibitors); 0 (Sulfinic Acids); 64B59K7U6Q (sulfuryl fluoride)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171016
[Lr] Data última revisão:
171016
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170724
[St] Status:MEDLINE


  3 / 969 MEDLINE  
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[PMID]:28498354
[Au] Autor:Yang Z; Abdellaoui H; He W; Xu J
[Ad] Endereço:State Key Laboratory of Chemical Resource Engineering, Department of Organic Chemistry, Faculty of Science, Beijing University of Chemical Technology, Beijing 100029, China. zhyang_2008a@163.com.
[Ti] Título:Ortho-Nitro Effect on the Diastereoselective Control in Sulfa-Staudinger and Staudinger Cycloadditions.
[So] Source:Molecules;22(5), 2017 May 12.
[Is] ISSN:1420-3049
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:The -nitro effect was discovered in sulfa-Staudinger cycloadditions of ethoxycarbonylsulfene with linear imines. When an -nitro group is present at the -aryl substituents of linear imines, the sulfa-Staudinger cycloadditions deliver -ß-sultams in considerable amounts, together with the predominant -ß-sultams. In other cases, the above sulfa-Staudinger cycloadditions give rise to -ß-sultams exclusively. Further mechanistic rationalization discloses that the -nitro effect is attributed to its strong electron-withdrawing inductive effect. Similarly, the -nitro effect also exists in Staudinger cycloadditions of ethoxycarbonyl ketene with the imines. The current research provides further insights into the diastereoselective control in sulfa-Staudinger and Staudinger cycloadditions.
[Mh] Termos MeSH primário: Reação de Cicloadição
Ésteres/química
Iminas/química
Cetonas/química
Sulfonamidas/química
Sulfonas/química
[Mh] Termos MeSH secundário: Espectroscopia de Ressonância Magnética
Malonatos/química
Espectrometria de Massas
Estereoisomerismo
Ácidos Sulfínicos/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Esters); 0 (Imines); 0 (Ketones); 0 (Malonates); 0 (Sulfinic Acids); 0 (Sulfonamides); 0 (Sulfones); 0 (beta-sultam); 0 (ethyl malonyl chloride); 7791-25-5 (sulfonyl chloride)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170627
[Lr] Data última revisão:
170627
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170513
[St] Status:MEDLINE


  4 / 969 MEDLINE  
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[PMID]:28421744
[Au] Autor:Prescott TAK; Panaretou B
[Ad] Endereço:Royal Botanic Gardens , Kew, Richmond, Surrey TW9 3AB, United Kingdom.
[Ti] Título:A Mini HIP HOP Assay Uncovers a Central Role for Copper and Zinc in the Antifungal Mode of Action of Allicin.
[So] Source:J Agric Food Chem;65(18):3659-3664, 2017 May 10.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Garlic contains the organosulfur compound allicin which exhibits potent antifungal activity. Here we demonstrate the use of a highly simplified yeast chemical genetic screen to characterize its mode of action. By screening 24 validated yeast gene deletion "signature" strains for which hypersensitivity is characteristic for common antifungal modes of action, yeast lacking the high affinity Cu transporter Ctr1 was found to be hypersensitive to allicin. Focusing on transition metal related genes identified two more hypersensitive strains lacking the Cu and Zn transcription factors Mac1 and Zap1. Hypersensitivity in these strains was reversed by the addition of Cu and Zn ions, respectively. The results suggest the antifungal activity of allicin is mediated through restricted Cu and Zn uptake or inhibition of Cu and Zn metalloproteins. As certain antimicrobial modes of action are much more common than others, the approach taken here provides a useful way to identify them early on.
[Mh] Termos MeSH primário: Antifúngicos/farmacologia
Cobre/metabolismo
Alho/química
Ensaios de Triagem em Larga Escala/métodos
Saccharomyces cerevisiae/efeitos dos fármacos
Ácidos Sulfínicos/farmacologia
Zinco/metabolismo
[Mh] Termos MeSH secundário: Saccharomyces cerevisiae/genética
Saccharomyces cerevisiae/metabolismo
Proteínas de Saccharomyces cerevisiae/genética
Proteínas de Saccharomyces cerevisiae/metabolismo
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antifungal Agents); 0 (Saccharomyces cerevisiae Proteins); 0 (Sulfinic Acids); 3C39BY17Y6 (allicin); 789U1901C5 (Copper); J41CSQ7QDS (Zinc)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170608
[Lr] Data última revisão:
170608
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170420
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jafc.7b00250


  5 / 969 MEDLINE  
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[PMID]:28259711
[Au] Autor:Wu X; Cai J; Li X; Li H; Li J; Bai X; Liu W; Han Y; Xu L; Zhang D; Wang H; Fan Z
[Ad] Endereço:Department of Otolaryngology-Head and Neck Surgery, First Affiliated Hospital of Wenzhou Medical University, Wenzhou, PR China; Department of Otolaryngology-Head and Neck Surgery, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, PR China.
[Ti] Título:Allicin protects against cisplatin-induced vestibular dysfunction by inhibiting the apoptotic pathway.
[So] Source:Eur J Pharmacol;805:108-117, 2017 Jun 15.
[Is] ISSN:1879-0712
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Cisplatin is an anticancer drug that causes the impairment of inner ear function as side effects, including hearing loss and balance dysfunction. The purpose of this study was to investigate the effects of allicin against cisplatin-induced vestibular dysfunction in mice and to make clear the mechanism underlying the protective effects of allicin on oto-vestibulotoxicity. Mice intraperitoneally injected with cisplatin exhibited vestibular dysfunction in swimming test, which agreed with impairment in vestibule. However, these impairments were significantly prevented by pre-treatment with allicin. Allicin markedly reduced cisplatin-activated expression of cleaved-caspase-3 in hair cells and vascular layer cells of utricule, saccule and ampulla, but also decreased AIF nuclear translocation of hair cells in utricule, saccule and ampulla. These results showed that allicin played an effective role in protecting vestibular dysfunction induced by cisplatin via inhibiting caspase-dependent and caspase-independent apoptotic pathways. Therefore, allicin may be useful in preventing oto-vestibulotoxicity mediated by cisplatin.
[Mh] Termos MeSH primário: Apoptose/efeitos dos fármacos
Cisplatino/efeitos adversos
Ácidos Sulfínicos/farmacologia
Vestíbulo do Labirinto/efeitos dos fármacos
Vestíbulo do Labirinto/patologia
[Mh] Termos MeSH secundário: Animais
Caspases/metabolismo
Feminino
Células Ciliadas Auditivas/efeitos dos fármacos
Células Ciliadas Auditivas/patologia
Masculino
Camundongos Endogâmicos C57BL
Vestíbulo do Labirinto/fisiopatologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Sulfinic Acids); 3C39BY17Y6 (allicin); EC 3.4.22.- (Caspases); Q20Q21Q62J (Cisplatin)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171108
[Lr] Data última revisão:
171108
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170306
[St] Status:MEDLINE


  6 / 969 MEDLINE  
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[PMID]:28212345
[Au] Autor:Cardoso MF; Gomes AT; Moreira CD; Simões MM; Neves MG; da Rocha DR; da Silva FC; Moreirinha C; Almeida A; Ferreira VF; Cavaleiro JA
[Ad] Endereço:Department of Chemistry and QOPNA, University of Aveiro, Aveiro 3810-193, Portugal. marianafcc83@hotmail.com.
[Ti] Título:Efficient Catalytic Oxidation of 3-Arylthio- and 3-Cyclohexylthio-lapachone Derivatives to New Sulfonyl Derivatives and Evaluation of Their Antibacterial Activities.
[So] Source:Molecules;22(2), 2017 Feb 16.
[Is] ISSN:1420-3049
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:New sulfonyl-lapachones were efficiently obtained through the catalytic oxidation of arylthio- and cyclohexylthio-lapachone derivatives with hydrogen peroxide in the presence of a Mn(III) porphyrin complex. The antibacterial activities of the non-oxidized and oxidized lapachone derivatives against the Gram-negative bacteria and the Gram-positive bacteria were evaluated after their incorporation into polyvinylpyrrolidone (PVP) micelles. The obtained results show that the PVP-formulations of the lapachones - and of the sulfonyl-lapachones and reduced the growth of .
[Mh] Termos MeSH primário: Antibacterianos/química
Antibacterianos/farmacologia
[Mh] Termos MeSH secundário: Antibacterianos/síntese química
Catálise
Bactérias Gram-Negativas/efeitos dos fármacos
Bactérias Gram-Positivas/efeitos dos fármacos
Espectroscopia de Ressonância Magnética
Testes de Sensibilidade Microbiana
Estrutura Molecular
Naftoquinonas/química
Naftoquinonas/farmacologia
Oxirredução
Ácidos Sulfínicos/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Naphthoquinones); 0 (Sulfinic Acids); 4707-32-8 (beta-lapachone)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170509
[Lr] Data última revisão:
170509
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170218
[St] Status:MEDLINE


  7 / 969 MEDLINE  
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[PMID]:28185379
[Au] Autor:Suksombat W; Nanon A; Meeprom C; Lounglawan P
[Ad] Endereço:School of Animal Production Technology, Institute of Agricultural Technology, Suranaree University of Technology, Nakhon Ratchasima, Thailand.
[Ti] Título:Feed degradability, rumen fermentation and blood metabolites in response to essential oil addition to fistulated non-lactating dairy cow diets.
[So] Source:Anim Sci J;88(9):1346-1351, 2017 Sep.
[Is] ISSN:1740-0929
[Cp] País de publicação:Australia
[La] Idioma:eng
[Ab] Resumo:The effects of essential oils (EOs) on ruminal nutrient disappearance, rumen fermentation and blood metabolites in fistulated non-lactating dairy cows were studied. Four fistulated non-lactaing dairy cows were used in a 4 × 4 Latin square design; the experiment consisted of four periods of 21 days in each period, with the first 14 days for adaptation followed by 7 days of measurement period. Animals were fed 3 kg/day of 21% crude protein (CP) concentrate and ad libitum corn silage. Treatments were: (i) control; (ii) 2 mL Allicin/cow/day; (iii) 2 mL zingiberene/cow/day; and (iv) 2 mL citral/cow/day. The results demonstrated that EOs increased dry matter and neutral detergent fiber degradabilities at 48 and 72 h, but had no effect on acid detergent fiber and CP degradabilities. EOs did not change ruminal pH, ammonia nitrogen, protozoa, volatile fatty acid concentrations and blood glucose but reduced blood urea nitrogen at 4 h.
[Mh] Termos MeSH primário: Ração Animal
Fenômenos Fisiológicos da Nutrição Animal/fisiologia
Bovinos/metabolismo
Bovinos/fisiologia
Dieta/veterinária
Fibras na Dieta/metabolismo
Suplementos Nutricionais
Digestão/fisiologia
Fermentação/fisiologia
Monoterpenos/administração & dosagem
Óleos Voláteis/administração & dosagem
Rúmen/metabolismo
Sesquiterpenos/administração & dosagem
Ácidos Sulfínicos/administração & dosagem
[Mh] Termos MeSH secundário: Animais
Glicemia
Nitrogênio da Ureia Sanguínea
Bovinos/sangue
Feminino
Concentração de Íons de Hidrogênio
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Blood Glucose); 0 (Dietary Fiber); 0 (Monoterpenes); 0 (Oils, Volatile); 0 (Sesquiterpenes); 0 (Sulfinic Acids); 3C39BY17Y6 (allicin); 8XOC63EI5F (zingiberene); T7EU0O9VPP (citral)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170211
[St] Status:MEDLINE
[do] DOI:10.1111/asj.12778


  8 / 969 MEDLINE  
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[PMID]:28139552
[Au] Autor:Lin XL; Liu Y; Liu M; Hu H; Pan Y; Fan XJ; Hu XM; Zou WW
[Ad] Endereço:Department of Pathology, Affiliated Hui Zhou hospital (The Third People's Hospital of Huizhou), Guangzhou Medical University, Huizhou, Guangdong, China (mainland).
[Ti] Título:Inhibition of Hydrogen Peroxide-Induced Human Umbilical Vein Endothelial Cells Aging by Allicin Depends on Sirtuin1 Activation.
[So] Source:Med Sci Monit;23:563-570, 2017 Jan 31.
[Is] ISSN:1643-3750
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND The abnormal activity of Sirtuin 1 (Sirt1) is closely related to the aging of vascular endothelial cells. As a bioactive molecule, allicin has antioxidant, anti-inflammatory, and lipid-regulating mechanisms. However, few reports about the relationship of allicin and Sirt1 have been published. In this study, we aimed to elucidate the effect of allicin on Human Umbilical Vein Endothelial Cells (HUVECs) aging induced by hydrogen peroxide (H2O2) and the role of Sirt1 in this phenomenon. MATERIAL AND METHODS HUVEC were exposed to H2O2 to establish the aging model. The expression of protein and RNA were detected by Western blot and Reverse transcription-quantitative polymerase chain reaction. The 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was used to assess cell viability. Sirt1 enzyme activity assay was used to analyze enzymatic activity. Reactive oxygen species was detected by dichlorofluorescein diacetate (DCFH-DA). Cell aging was detected by Senescence ß-Galactosidase (SA-ß-gal) staining. RESULTS Results of this study revealed that pretreating HUVECs with 5 ng/mL allicin before exposure to H2O2 resulted in increased cell viability and reduced reactive oxygen species generation. Western blot and quantitative real-time polymerase chain reaction (qRT-PCR) analysis showed that H2O2 attenuated the phosphorylation and activation of Sirt1 and increased the expression of plasminogen activator inhibitor-1(PAI-1) protein. Moreover, H2O2 also promoted HUVEC aging. These effects were significantly alleviated by 5 ng/mL allicin co-treatment. Furthermore, the anti-aging effects of allicin were abolished by the Sirt1 inhibitor nicotinamide (NAM). CONCLUSIONS Overall, the results demonstrated that allicin protects HUVECs from H2O2-induced oxidative stress and aging via the activation of Sirt1.
[Mh] Termos MeSH primário: Senescência Celular/efeitos dos fármacos
Células Endoteliais da Veia Umbilical Humana/efeitos dos fármacos
Sirtuína 1/farmacologia
Ácidos Sulfínicos/farmacologia
[Mh] Termos MeSH secundário: Antioxidantes/farmacologia
Sobrevivência Celular/efeitos dos fármacos
Células Cultivadas
Interações Medicamentosas
Células Endoteliais
Células Endoteliais da Veia Umbilical Humana/citologia
Seres Humanos
Peróxido de Hidrogênio/farmacologia
Niacinamida/farmacologia
Óxido Nítrico/metabolismo
Estresse Oxidativo/efeitos dos fármacos
Fosforilação
Substâncias Protetoras/farmacologia
Espécies Reativas de Oxigênio/metabolismo
beta-Galactosidase/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antioxidants); 0 (Protective Agents); 0 (Reactive Oxygen Species); 0 (Sulfinic Acids); 25X51I8RD4 (Niacinamide); 31C4KY9ESH (Nitric Oxide); 3C39BY17Y6 (allicin); BBX060AN9V (Hydrogen Peroxide); EC 3.2.1.23 (beta-Galactosidase); EC 3.5.1.- (Sirtuin 1)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170201
[St] Status:MEDLINE


  9 / 969 MEDLINE  
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[PMID]:27979174
[Au] Autor:Tocmo R; Wu Y; Liang D; Fogliano V; Huang D
[Ad] Endereço:Food Science and Technology Programme, Department of Chemistry, National University of Singapore, 117543 Singapore, Singapore.
[Ti] Título:Boiling enriches the linear polysulfides and the hydrogen sulfide-releasing activity of garlic.
[So] Source:Food Chem;221:1867-1873, 2017 Apr 15.
[Is] ISSN:0308-8146
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Garlic is rich in polysulfides, and some of them can be H S donors. This study was conducted to explore the effect of cooking on garlic's organopolysulfides and H S-releasing activity. Garlic bulbs were crushed and boiled for a period ranging from 3 to 30min and the solvent extracts were analyzed by GC-MS/FID and HPLC. A cell-based assay was used to measure the H S-releasing activity of the extracts. Results showed that the amounts of allyl polysulfides increased in crushed garlic boiled for 6-10min; however, prolonging the thermal treatment to 20 or 30min decreased their concentrations. Data of the H S-releasing activity, expressed as diallyl trisulfide equivalents (DATS-E), parallel this trend, being significantly higher at 6 and 10min boiling. Our results showed enhancement of H S-releasing activity upon moderate boiling, suggesting that shorter cooking time may maximize its health benefits as a dietary source of natural H S donors.
[Mh] Termos MeSH primário: Culinária
Alho/química
Sulfeto de Hidrogênio/química
Sulfetos/química
[Mh] Termos MeSH secundário: Compostos Alílicos/química
Cromatografia Líquida de Alta Pressão
Cromatografia Líquida
Cromatografia Gasosa-Espectrometria de Massas
Raízes de Plantas/química
Ácidos Sulfínicos/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Allyl Compounds); 0 (Sulfides); 0 (Sulfinic Acids); 0ZO1U5A3XX (diallyl trisulfide); 3C39BY17Y6 (allicin); 9080-49-3 (polysulfide); YY9FVM7NSN (Hydrogen Sulfide)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:171003
[Lr] Data última revisão:
171003
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161217
[St] Status:MEDLINE


  10 / 969 MEDLINE  
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[PMID]:27939830
[Au] Autor:Mellado-García P; Maisanaba S; Puerto M; Prieto AI; Marcos R; Pichardo S; Cameán AM
[Ad] Endereço:Area of Toxicology, Faculty of Pharmacy, Universidad de Sevilla, Profesor García González n°2, 41012 Seville, Spain.
[Ti] Título:In vitro toxicological assessment of an organosulfur compound from Allium extract: Cytotoxicity, mutagenicity and genotoxicity studies.
[So] Source:Food Chem Toxicol;99:231-240, 2017 Jan.
[Is] ISSN:1873-6351
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Garlic (Allium sativum) and onion (Allium cepa) are being used in the food industry as flavoring but also for their antimicrobial activities. These activities are mainly derived from the organosulfur compounds (OSCs). Propyl propane thiosulfinate (PTS) is an OSC with potential use in the active packaging, but its safety should be guaranteed before being commercialized. The aim of this work was to investigate for the first time the cytotoxicity of PTS as well as its in vitro mutagenic/genotoxic potential using the following battery of genotoxicity tests:(1)the bacterial reverse-mutation assay in S. typhimurium (Ames test, OECD 471, 1997); (2) the micronucleus test (MN, OECD 487, 2016); (3) the mouse lymphoma thymidine-kinase assay (MLA, OECD 476, 2015), and (4) the comet assay (standard and modified with restriction enzymes). The results revealed that PTS was not mutagenic neither in the Ames test nor in MLA. However, genotoxic effects were recorded in the MN test on mammalian cells (L5178YTk cells) after PTS exposure at the highest concentration tested (17.25 µM) without S9, and also its metabolites (+S9, from 20 µM). Moreover, in the comet assay, PTS induced DNA breaks damage in Caco-2 cells at the highest concentration tested (280 µM) but it did not induce oxidative DNA damage.
[Mh] Termos MeSH primário: Sobrevivência Celular/efeitos dos fármacos
Dano ao DNA/efeitos dos fármacos
Alho/química
Linfoma/patologia
Extratos Vegetais/toxicidade
Salmonella typhimurium/efeitos dos fármacos
Ácidos Sulfínicos/toxicidade
[Mh] Termos MeSH secundário: Animais
Células CACO-2
Ensaio Cometa
Seres Humanos
Linfoma/tratamento farmacológico
Camundongos
Testes para Micronúcleos
Mutação/genética
Timidina Quinase/genética
Timidina Quinase/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Plant Extracts); 0 (Sulfinic Acids); 0 (propyl thiosulfinate oxide); EC 2.7.1.21 (Thymidine Kinase)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170314
[Lr] Data última revisão:
170314
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161213
[St] Status:MEDLINE



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