Base de dados : MEDLINE
Pesquisa : D01.029.260.877.800.900 [Categoria DeCS]
Referências encontradas : 161 [refinar]
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  1 / 161 MEDLINE  
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[PMID]:28553941
[Au] Autor:Riglar DT; Giessen TW; Baym M; Kerns SJ; Niederhuber MJ; Bronson RT; Kotula JW; Gerber GK; Way JC; Silver PA
[Ad] Endereço:Department of Systems Biology, Harvard Medical School, Boston, Massachusetts, USA.
[Ti] Título:Engineered bacteria can function in the mammalian gut long-term as live diagnostics of inflammation.
[So] Source:Nat Biotechnol;35(7):653-658, 2017 Jul.
[Is] ISSN:1546-1696
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Bacteria can be engineered to function as diagnostics or therapeutics in the mammalian gut but commercial translation of technologies to accomplish this has been hindered by the susceptibility of synthetic genetic circuits to mutation and unpredictable function during extended gut colonization. Here, we report stable, engineered bacterial strains that maintain their function for 6 months in the mouse gut. We engineered a commensal murine Escherichia coli strain to detect tetrathionate, which is produced during inflammation. Using our engineered diagnostic strain, which retains memory of exposure in the gut for analysis by fecal testing, we detected tetrathionate in both infection-induced and genetic mouse models of inflammation over 6 months. The synthetic genetic circuits in the engineered strain were genetically stable and functioned as intended over time. The durable performance of these strains confirms the potential of engineered bacteria as living diagnostics.
[Mh] Termos MeSH primário: Escherichia coli/genética
Escherichia coli/metabolismo
Gastroenterite/diagnóstico
Gastroenterite/microbiologia
Microbioma Gastrointestinal
Ácido Tetratiônico/metabolismo
[Mh] Termos MeSH secundário: Animais
Sobrevivência Celular
Escherichia coli/isolamento & purificação
Feminino
Engenharia Genética/métodos
Intestinos
Camundongos
Camundongos Endogâmicos C57BL
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
8V1L8R19JH (Tetrathionic Acid)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:171027
[Lr] Data última revisão:
171027
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170530
[St] Status:MEDLINE
[do] DOI:10.1038/nbt.3879


  2 / 161 MEDLINE  
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[PMID]:27997871
[Au] Autor:Sulonen ML; Lakaniemi AM; Kokko ME; Puhakka JA
[Ad] Endereço:Department of Chemistry and Bioengineering, Tampere University of Technology, Tampere, Finland. Electronic address: mira.sulonen@tut.fi.
[Ti] Título:The effect of anode potential on bioelectrochemical and electrochemical tetrathionate degradation.
[So] Source:Bioresour Technol;226:173-180, 2017 Feb.
[Is] ISSN:1873-2976
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The effect of poised anode potential on electricity production and tetrathionate degradation was studied in two-chamber flow-through electrochemical (ES) and bioelectrochemical systems (BES). The minimum anode potential (vs. Ag/AgCl) for positive current generation was 0.3V in BES and 0.5V in the abiotic ES. The anode potential required to obtain average current density above 70mAm was 0.4V in BES and above 0.7V in ES. ES provided higher coulombic efficiency, but the average tetrathionate degradation rate remained significantly higher in BES (above 110mgL d ) than in the abiotic ES (below 35mgL d ). This study shows that at anode potentials below 0.7V, the electrochemical tetrathionate degradation is only efficient with microbial catalyst and that significantly higher tetrathionate degradation rates can be obtained with bioelectrochemical systems than with electrochemical systems at the tested anode potentials.
[Mh] Termos MeSH primário: Fontes de Energia Bioelétrica
Técnicas Eletroquímicas
Ácido Tetratiônico/química
[Mh] Termos MeSH secundário: Catálise
Eletrodos
Concentração de Íons de Hidrogênio
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
8V1L8R19JH (Tetrathionic Acid)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161221
[St] Status:MEDLINE


  3 / 161 MEDLINE  
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[PMID]:27318661
[Au] Autor:Sulonen ML; Lakaniemi AM; Kokko ME; Puhakka JA
[Ad] Endereço:Department of Chemistry and Bioengineering, Tampere University of Technology, Tampere, Finland. Electronic address: mira.sulonen@tut.fi.
[Ti] Título:Long-term stability of bioelectricity generation coupled with tetrathionate disproportionation.
[So] Source:Bioresour Technol;216:876-82, 2016 Sep.
[Is] ISSN:1873-2976
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:To prevent uncontrolled acidification of the environment, reduced inorganic sulfur compounds (RISCs) can be bioelectrochemically removed from water streams. The long-term stability of bioelectricity production from tetrathionate (S4O6(2-)) was studied in highly acidic conditions (pH<2.5) in two-chamber fed-batch microbial fuel cells (MFCs). The maximum current density was improved from previously reported 80mAm(-2) to 225mAm(-2) by optimizing the external resistance. The observed reaction products of tetrathionate disproportionation were sulfate and elemental sulfur. In long-term run, stable electricity production was obtained for over 700days with the average current density of 150mAm(-2). The internal resistance of the MFC decreased over time and no biofouling was observed. This study shows that tetrathionate is an efficient substrate also for long-term bioelectricity production.
[Mh] Termos MeSH primário: Fontes de Energia Bioelétrica
Ácido Tetratiônico/química
[Mh] Termos MeSH secundário: Eletricidade
Enxofre/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
70FD1KFU70 (Sulfur); 8V1L8R19JH (Tetrathionic Acid)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170110
[Lr] Data última revisão:
170110
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160620
[St] Status:MEDLINE


  4 / 161 MEDLINE  
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[PMID]:26252619
[Au] Autor:Narayan V; Kudva AK; Prabhu KS
[Ad] Endereço:Department of Veterinary and Biomedical Sciences, Center for Molecular Immunology and Infectious Disease, and Center for Molecular Toxicology and Carcinogenesis, The Pennsylvania State University , University Park, Pennsylvania 16802, United States.
[Ti] Título:Reduction of Tetrathionate by Mammalian Thioredoxin Reductase.
[So] Source:Biochemistry;54(33):5121-4, 2015 Aug 25.
[Is] ISSN:1520-4995
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Tetrathionate, a polythionate oxidation product of microbial hydrogen sulfide and reactive oxygen species from immune cells in the gut, serves as a terminal electron acceptor to confer a growth advantage for Salmonella and other enterobacteria. Here we show that the rat liver selenoenzyme thioredoxin reductase (Txnrd1, TR1) efficiently reduces tetrathionate in vitro. Furthermore, lysates of selenium-supplemented murine macrophages also displayed activity toward tetrathionate, while cells lacking TR1 were unable to reduce tetrathionate. These studies suggest that upregulation of TR1 expression, via selenium supplementation, may modulate the gut microbiome, particularly during inflammation, by regulating the levels of tetrathionate.
[Mh] Termos MeSH primário: Ácido Tetratiônico/metabolismo
Tiorredoxina Dissulfeto Redutase/metabolismo
[Mh] Termos MeSH secundário: Animais
Fígado/enzimologia
Oxirredução
Ratos
Selênio/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL
[Nm] Nome de substância:
8V1L8R19JH (Tetrathionic Acid); EC 1.8.1.9 (Thioredoxin-Disulfide Reductase); H6241UJ22B (Selenium)
[Em] Mês de entrada:1511
[Cu] Atualização por classe:161019
[Lr] Data última revisão:
161019
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150808
[St] Status:MEDLINE
[do] DOI:10.1021/acs.biochem.5b00620


  5 / 161 MEDLINE  
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[PMID]:26252376
[Au] Autor:van Bemmelen MX; Huser D; Gautschi I; Schild L
[Ad] Endereço:Department of Pharmacology & Toxicology, Faculty of Biology and Medicine, Lausanne University, Lausanne, Switzerland.
[Ti] Título:The Human Acid-Sensing Ion Channel ASIC1a: Evidence for a Homotetrameric Assembly State at the Cell Surface.
[So] Source:PLoS One;10(8):e0135191, 2015.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The chicken acid-sensing ion channel ASIC1 has been crystallized as a homotrimer. We address here the oligomeric state of the functional ASIC1 in situ at the cell surface. The oligomeric states of functional ASIC1a and mutants with additional cysteines introduced in the extracellular pore vestibule were resolved on SDS-PAGE. The functional ASIC1 complexes were stabilized at the cell surface of Xenopus laevis oocytes or CHO cells either using the sulfhydryl crosslinker BMOE, or sodium tetrathionate (NaTT). Under these different crosslinking conditions ASIC1a migrates as four distinct oligomeric states that correspond by mass to multiples of a single ASIC1a subunit. The relative importance of each of the four ASIC1a oligomers was critically dependent on the availability of cysteines in the transmembrane domain for crosslinking, consistent with the presence of ASIC1a homo-oligomers. The expression of ASIC1a monomers, trimeric or tetrameric concatemeric cDNA constructs resulted in functional channels. The resulting ASIC1a complexes are resolved as a predominant tetramer over the other oligomeric forms, after stabilization with BMOE or NaTT and SDS-PAGE/western blot analysis. Our data identify a major ASIC1a homotetramer at the surface membrane of the cell expressing functional ASIC1a channel.
[Mh] Termos MeSH primário: Canais Iônicos Sensíveis a Ácido/química
Membrana Celular/química
[Mh] Termos MeSH secundário: Animais
Células CHO
Cricetinae
Cricetulus
Reagentes para Ligações Cruzadas/química
Cisteína/química
DNA Complementar/química
Seres Humanos
Mutagênese Sítio-Dirigida
Mutação
Oócitos/citologia
Multimerização Proteica
Estrutura Terciária de Proteína
Ácido Tetratiônico/química
Xenopus laevis
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (ASIC1 protein, human); 0 (Acid Sensing Ion Channels); 0 (Cross-Linking Reagents); 0 (DNA, Complementary); 8V1L8R19JH (Tetrathionic Acid); K848JZ4886 (Cysteine)
[Em] Mês de entrada:1605
[Cu] Atualização por classe:150814
[Lr] Data última revisão:
150814
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150808
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0135191


  6 / 161 MEDLINE  
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[PMID]:25651337
[Au] Autor:Ji C; Yan X; Horváth AK; Pan C; Zhao Y; Gao Q
[Ad] Endereço:School of Chemical Engineering, China University of Mining and Technology , Xuzhou 221116, People's Republic of China.
[Ti] Título:Comprehensive simultaneous kinetic study of sulfitolysis and thiosulfatolysis of tetrathionate ion: unravelling the unique pH dependence of thiosulfatolysis.
[So] Source:J Phys Chem A;119(8):1238-45, 2015 Feb 26.
[Is] ISSN:1520-5215
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The kinetics of the reactions of tetrathionate with S(IV) species and with thiosulfate in slightly acidic and neutral media were studied concurrently at 25.0 ± 0.1 °C by simultaneous high-performance liquid chromatography monitoring of the concentrations of polythionates (including trithionate, tetrathionate, and pentathionate), thiosulfate, and sulfite. The tetrathionate-sulfite and tetrathionate-thiosulfate reactions were found to be first-order with respect to both reactants. The tetrathionate-sulfite reaction was found to be pH-dependent under the conditions studied. In contrast, the tetrathionate-thiosulfate reaction was experimentally demonstrated to be pH-independent at neutral medium, where the pKa2 value of sulfurous acid plays a key role, whereas under slightly acidic conditions, between pH 4 and 5 the consumption of tetrathionate during the course of reaction was found to become pH-dependent. We show that the pH dependencies in both systems can be readily explained by the reactivity difference between sulfite and bisulfite toward the ß-sulfur of the tetrathionate. A simple two-step kinetic model incorporating the protonation equilibrium of sulfite is proposed on the basis of the simultaneous evaluation of the kinetic curves of the two systems, which allowed us to determine reliable rate coefficients for both the forward and backward reactions. Furthermore, the powerful ability of simultaneously evaluating the two chemical systems to yield reliable rate coefficients of the kinetic model is demonstrated.
[Mh] Termos MeSH primário: Íons/química
Sulfitos/química
Ácido Tetratiônico/química
Tiossulfatos/química
[Mh] Termos MeSH secundário: Cromatografia Líquida de Alta Pressão
Concentração de Íons de Hidrogênio
Cinética
Modelos Químicos
Prótons
Enxofre/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Ions); 0 (Protons); 0 (Sulfites); 0 (Thiosulfates); 0 (polythionates); 70FD1KFU70 (Sulfur); 8V1L8R19JH (Tetrathionic Acid)
[Em] Mês de entrada:1602
[Cu] Atualização por classe:150226
[Lr] Data última revisão:
150226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150205
[St] Status:MEDLINE
[do] DOI:10.1021/jp5108119


  7 / 161 MEDLINE  
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[PMID]:25463232
[Au] Autor:Sulonen ML; Kokko ME; Lakaniemi AM; Puhakka JA
[Ad] Endereço:Department of Chemistry and Bioengineering, Tampere University of Technology, Tampere, Finland. Electronic address: mira.sulonen@tut.fi.
[Ti] Título:Electricity generation from tetrathionate in microbial fuel cells by acidophiles.
[So] Source:J Hazard Mater;284:182-9, 2015 Mar 02.
[Is] ISSN:1873-3336
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Inorganic sulfur compounds, such as tetrathionate, are often present in mining process and waste waters. The biodegradation of tetrathionate was studied under acidic conditions in aerobic batch cultivations and in anaerobic anodes of two-chamber flow-through microbial fuel cells (MFCs). All four cultures originating from biohydrometallurgical process waters from multimetal ore heap bioleaching oxidized tetrathionate aerobically at pH below 3 with sulfate as the main soluble metabolite. In addition, all cultures generated electricity from tetrathionate in MFCs at pH below 2.5 with ferric iron as the terminal cathodic electron acceptor. The maximum current and power densities during MFC operation and in the performance analysis were 79.6 mA m(-2) and 13.9 mW m(-2) and 433 mA m(-2) and 17.6 mW m(-2), respectively. However, the low coulombic efficiency (below 5%) indicates that most of the electrons were directed to other processes, such as aerobic oxidation of tetrathionate and unmeasured intermediates. The microbial community analysis revealed that the dominant species both in the anolyte and on the anode electrode surface of the MFCs were Acidithiobacillus spp. and Ferroplasma spp. This study provides a proof of concept that tetrathionate serves as electron donor for biological electricity production in the pH range of 1.2-2.5.
[Mh] Termos MeSH primário: Acidithiobacillus/metabolismo
Fontes de Energia Bioelétrica
Eletroquímica/métodos
Ácido Tetratiônico/química
Tionas/química
[Mh] Termos MeSH secundário: Biodegradação Ambiental
Eletricidade
Eletrodos
Elétrons
Concentração de Íons de Hidrogênio
Microbiota
Oxirredução
Oxigênio/química
Enxofre/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Thiones); 70FD1KFU70 (Sulfur); 8V1L8R19JH (Tetrathionic Acid); S88TT14065 (Oxygen)
[Em] Mês de entrada:1508
[Cu] Atualização por classe:141216
[Lr] Data última revisão:
141216
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:141203
[St] Status:MEDLINE


  8 / 161 MEDLINE  
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[PMID]:24727223
[Au] Autor:Yu Y; Liu X; Wang H; Li X; Lin J
[Ad] Endereço:State Key Laboratory of Microbial Technology, Shandong University, Jinan, People's Republic of China.
[Ti] Título:Construction and characterization of tetH overexpression and knockout strains of Acidithiobacillus ferrooxidans.
[So] Source:J Bacteriol;196(12):2255-64, 2014 Jun.
[Is] ISSN:1098-5530
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Acidithiobacillus ferrooxidans is a major participant in consortia of microorganisms used for bioleaching. It can obtain energy from the oxidation of Fe(2+), H2, S(0), and various reduced inorganic sulfur compounds (RISCs). Tetrathionate is a key intermediate during RISC oxidation, hydrolyzed by tetrathionate hydrolase (TetH), and used as sole energy source. In this study, a tetH knockout (ΔtetH) mutant and a tetH overexpression strain were constructed and characterized. The tetH overexpression strain grew better on sulfur and tetrathionate and possessed a higher rate of tetrathionate utilization and TetH activity than the wild type. However, its cell yields on tetrathionate were much lower than those on sulfur. The ΔtetH mutant could not grow on tetrathionate but could proliferate on sulfur with a lower cell yield than the wild type's, which indicated that tetrathionate hydrolysis is mediated only by TetH, encoded by tetH. The ΔtetH mutant could survive in ferrous medium with an Fe(2+) oxidation rate similar to that of the wild type. For the tetH overexpression strain, the rate was relatively higher than that of the wild type. The reverse transcription-quantitative PCR (qRT-PCR) results showed that tetH and doxD2 acted synergistically, and doxD2 was considered important in thiosulfate metabolism. Of the two sqr genes, AFE_0267 seemed to play as important a role in sulfide oxidation as AFE_1792. This study not only provides a substantial basis for studying the function of the tetH gene but also may serve as a model to clarify other candidate genes involved in sulfur oxidation in this organism.
[Mh] Termos MeSH primário: Acidithiobacillus/metabolismo
Proteínas de Bactérias/metabolismo
Regulação Bacteriana da Expressão Gênica/fisiologia
[Mh] Termos MeSH secundário: Acidithiobacillus/genética
Proteínas de Bactérias/genética
Metabolismo Energético/fisiologia
Deleção de Genes
Oxirredução
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Ácido Tetratiônico/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Bacterial Proteins); 8V1L8R19JH (Tetrathionic Acid)
[Em] Mês de entrada:1412
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140415
[St] Status:MEDLINE
[do] DOI:10.1128/JB.01472-13


  9 / 161 MEDLINE  
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[PMID]:23637594
[Au] Autor:Rivera-Chávez F; Winter SE; Lopez CA; Xavier MN; Winter MG; Nuccio SP; Russell JM; Laughlin RC; Lawhon SD; Sterzenbach T; Bevins CL; Tsolis RM; Harshey R; Adams LG; Bäumler AJ
[Ad] Endereço:Department of Medical Microbiology and Immunology, School of Medicine, University of California, Davis, Davis, California, United States of America.
[Ti] Título:Salmonella uses energy taxis to benefit from intestinal inflammation.
[So] Source:PLoS Pathog;9(4):e1003267, 2013.
[Is] ISSN:1553-7374
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Chemotaxis enhances the fitness of Salmonella enterica serotype Typhimurium (S. Typhimurium) during colitis. However, the chemotaxis receptors conferring this fitness advantage and their cognate signals generated during inflammation remain unknown. Here we identify respiratory electron acceptors that are generated in the intestinal lumen as by-products of the host inflammatory response as in vivo signals for methyl-accepting chemotaxis proteins (MCPs). Three MCPs, including Trg, Tsr and Aer, enhanced the fitness of S. Typhimurium in a mouse colitis model. Aer mediated chemotaxis towards electron acceptors (energy taxis) in vitro and required tetrathionate respiration to confer a fitness advantage in vivo. Tsr mediated energy taxis towards nitrate but not towards tetrathionate in vitro and required nitrate respiration to confer a fitness advantage in vivo. These data suggest that the energy taxis receptors Tsr and Aer respond to distinct in vivo signals to confer a fitness advantage upon S. Typhimurium during inflammation by enabling this facultative anaerobic pathogen to seek out favorable spatial niches containing host-derived electron acceptors that boost its luminal growth.
[Mh] Termos MeSH primário: Proteínas de Bactérias/metabolismo
Quimiotaxia
Colite/microbiologia
Metabolismo Energético
Proteínas de Membrana/metabolismo
Salmonelose Animal/microbiologia
Salmonella typhimurium/patogenicidade
[Mh] Termos MeSH secundário: Animais
Proteínas de Transporte/metabolismo
Colite/imunologia
Transporte de Elétrons
Feminino
Inflamação
Intestinos/metabolismo
Intestinos/microbiologia
Proteínas Quimiotáticas Aceptoras de Metil
Camundongos
Camundongos Endogâmicos C57BL
Camundongos Endogâmicos CBA
Neutrófilos/imunologia
Nitratos/metabolismo
Espécies Reativas de Oxigênio/metabolismo
Receptores de Superfície Celular/metabolismo
Salmonelose Animal/imunologia
Salmonella typhimurium/imunologia
Salmonella typhimurium/fisiologia
Ácido Tetratiônico/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Carrier Proteins); 0 (Membrane Proteins); 0 (Methyl-Accepting Chemotaxis Proteins); 0 (Nitrates); 0 (Reactive Oxygen Species); 0 (Receptors, Cell Surface); 0 (Tsr protein, Bacteria); 8V1L8R19JH (Tetrathionic Acid)
[Em] Mês de entrada:1401
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:130503
[St] Status:MEDLINE
[do] DOI:10.1371/journal.ppat.1003267


  10 / 161 MEDLINE  
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[PMID]:23421726
[Au] Autor:Liu YW; Denkmann K; Kosciow K; Dahl C; Kelly DJ
[Ad] Endereço:Department of Molecular Biology and Biotechnology, The University of Sheffield, Firth Court, Western Bank, Sheffield, S10 2TN, UK.
[Ti] Título:Tetrathionate stimulated growth of Campylobacter jejuni identifies a new type of bi-functional tetrathionate reductase (TsdA) that is widely distributed in bacteria.
[So] Source:Mol Microbiol;88(1):173-88, 2013 Apr.
[Is] ISSN:1365-2958
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Tetrathionate (S4 O6 (2-) ) is used by some bacteria as an electron acceptor and can be produced in the vertebrate intestinal mucosa from the oxidation of thiosulphate (S2 O3 (2-) ) by reactive oxygen species during inflammation. Surprisingly, growth of the microaerophilic mucosal pathogen Campylobacter jejuni under oxygen-limited conditions was stimulated by tetrathionate, although it does not possess any known type of tetrathionate reductase. Here, we identify a dihaem cytochrome c (C8j_0815; TsdA) as the enzyme responsible. Kinetic studies with purified recombinant C. jejuni TsdA showed it to be a bifunctional tetrathionate reductase/thiosulphate dehydrogenase with a high affinity for tetrathionate. A tsdA null mutant still slowly reduced, but could not grow on, tetrathionate under oxygen limitation, lacked thiosulphate-dependent respiration and failed to convert thiosulphate to tetrathionate microaerobically. A TsdA paralogue (C8j_0040), lacking the unusual His-Cys haem ligation of TsdA, had low thiosulphate dehydrogenase and tetrathionate reductase activities. Our data highlight a hitherto unrecognized capacity of C. jejuni to use tetrathionate and thiosulphate in its energy metabolism, which may promote growth in the host. Moreover, as TsdA represents a new class of tetrathionate reductase that is widely distributed among bacteria, we predict that energy conserving tetrathionate respiration is far more common than currently appreciated.
[Mh] Termos MeSH primário: Campylobacter jejuni/enzimologia
Campylobacter jejuni/crescimento & desenvolvimento
Oxirredutases/metabolismo
Ácido Tetratiônico/farmacologia
[Mh] Termos MeSH secundário: Aerobiose/efeitos dos fármacos
Biocatálise/efeitos dos fármacos
Campylobacter jejuni/citologia
Campylobacter jejuni/efeitos dos fármacos
Citocromos c/metabolismo
Elétrons
Formiatos/farmacologia
Cinética
Modelos Biológicos
Mutação/genética
NADH NADPH Oxirredutases/metabolismo
Oxirredução/efeitos dos fármacos
Oxigênio/farmacologia
Proteínas Recombinantes/metabolismo
Análise Espectral
Tiossulfatos/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Formates); 0 (Recombinant Proteins); 0 (Thiosulfates); 8V1L8R19JH (Tetrathionic Acid); 9007-43-6 (Cytochromes c); EC 1.- (Oxidoreductases); EC 1.6.- (NADH, NADPH Oxidoreductases); EC 1.6.99.- (ferricyanide reductase); EC 1.7.- (tetrathionate reductase); EC 1.8.2.2 (thiosulfate dehydrogenase); S88TT14065 (Oxygen)
[Em] Mês de entrada:1309
[Cu] Atualização por classe:150113
[Lr] Data última revisão:
150113
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:130221
[St] Status:MEDLINE
[do] DOI:10.1111/mmi.12176



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