Base de dados : MEDLINE
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[PMID]:29458475
[Au] Autor:Gan L; Zhang H; Long X; Tian J; Wang Z; Zhang Y; Dai Y; Tian Y
[Ad] Endereço:1​Key Laboratory of Leather Chemistry and Engineering, Ministry of Education and College of Light Industry, Textile & Food Engineering, Sichuan University, Chengdu 610065, PR China.
[Ti] Título:Ornithinibacillus salinisoli sp. nov., a moderately halophilic bacterium isolated from a saline-alkali soil.
[So] Source:Int J Syst Evol Microbiol;68(3):769-775, 2018 Mar.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A taxonomic study was performed on strain LCB256 , which was isolated from a saline-alkali soil sample taken from northwestern China. Cells of strain LCB256 were Gram-stain-positive, aerobic, rod-shaped and grew at 3-17 % (w/v) NaCl (optimum 10-15 %), 10-52 °C (optimum 25-30 °C) and pH 7.0-9.0 (optimum 8.0). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain LCB256 was most closely related to the two genera of Ornithinibacillus and Oceanobacillus, showing highest sequence similarity to Oceanobacillus limi KCTC 13823 (97.8 %) and Ornithinibacillus bavariensis WSBC 24001 (97.2 %). The peptidoglycan amino acid type was found to be A4ß and the major respiratory quinone was determined to be MK-7. The polar lipid profile of strain LCB256 contained diphosphatidylglycerol, phosphatidylglycerol, one unidentified phospholipid and two unidentified aminolipids. The dominant cellular fatty acids were anteiso-C15 : 0 and iso-C15 : 0. The G+C content of genomic DNA was 39.3 mol%. DNA-DNA relatedness values between strain LCB256 and Ornithinibacillus halophilus KCTC 13822 and Oceanobacillus limi KCTC 13823 were 46.2 and 34.8 %, respectively. Based on this polyphasic taxonomic study, a novel species of the genus Ornithinibacillus, Ornithinibacillussalinisoli sp. nov. is proposed. The type strain is LCB256 (=CGMCC 1.15809 =KCTC 33862 ).
[Mh] Termos MeSH primário: Bacillaceae/classificação
Filogenia
Microbiologia do Solo
[Mh] Termos MeSH secundário: Álcalis
Bacillaceae/genética
Bacillaceae/isolamento & purificação
Técnicas de Tipagem Bacteriana
Composição de Bases
China
DNA Bacteriano/genética
Ácidos Graxos/química
Hibridização de Ácido Nucleico
Peptidoglicano/química
Fosfolipídeos/química
RNA Ribossômico 16S/genética
Salinidade
Análise de Sequência de DNA
Vitamina K 2/análogos & derivados
Vitamina K 2/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Alkalies); 0 (DNA, Bacterial); 0 (Fatty Acids); 0 (Peptidoglycan); 0 (Phospholipids); 0 (RNA, Ribosomal, 16S); 11032-49-8 (Vitamin K 2); 8427BML8NY (vitamin MK 7)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180308
[Lr] Data última revisão:
180308
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180221
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.002580


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[PMID]:29300160
[Au] Autor:Gan L; Zhang H; Tian J; Li X; Long X; Zhang Y; Dai Y; Tian Y
[Ad] Endereço:1​Key Laboratory of Leather Chemistry and Engineering, Ministry of Education and College of Light Industry, Textile & Food Engineering, Sichuan University, Chengdu 610065, PR China.
[Ti] Título:Planococcus salinus sp. nov., a moderately halophilic bacterium isolated from a saline-alkali soil.
[So] Source:Int J Syst Evol Microbiol;68(2):589-595, 2018 Feb.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A novel aerobic, Gram-stain-positive, motile, moderately halophilic and coccoid bacterial strain, designated LCB217 , was isolated from a saline-alkali soil in north-western China and identified using a polyphasic taxonomic approach. Growth occurred with 3-15 % (w/v) NaCl (optimum 3-5 %), at 10-45 °C (optimum 30 °C) and at pH 7.0-9.0 (optimum pH 9.0). Strain LCB217 contained MK-7 and MK-8 as the predominant menaquinones and anteiso-C15 : 0, iso-C14 : 0 and iso-C16 : 0 as the major fatty acids. The polar lipids from strain LCB217 consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, one unidentified phospholipid, one unidentified aminophospholipid and one unidentified lipid. The peptidoglycan type was A4α (l-Lys-d-Glu). Phylogenetic analysis of the 16S rRNA gene sequence showed that strain LCB217 belonged to the genus Planococcus and was closely related to the type strains Planococcus plakortidis AS/ASP6 (II) (98.2 % similarity), Planococcus maitriensis S1 (97.7 %) and Planococcus salinarum ISL-16 (97.2 %). The G+C content of the genomic DNA was 49.4 mol%. DNA-DNA relatedness values between strain LCB217 andPlanococcusplakortidis AS/ASP6 (II) , Planococcusmaitriensis S1 andPlanococcussalinarum ISL-16 were 29.5, 38.1 and 39.5 %, respectively. On the basis of the phenotypic, phylogenetic and genomic data, strain LCB217 represents a novel species of the genus Planococcus, for which the name Planococcus salinus sp. nov. is proposed. The type strain is LCB217 (=CGMCC 1.15685 =KCTC 33861 ).
[Mh] Termos MeSH primário: Filogenia
Bactérias Planococcus/classificação
Microbiologia do Solo
[Mh] Termos MeSH secundário: Álcalis
Técnicas de Tipagem Bacteriana
Composição de Bases
China
DNA Bacteriano/genética
Ácidos Graxos/química
Hibridização de Ácido Nucleico
Fosfolipídeos/química
Bactérias Planococcus/genética
Bactérias Planococcus/isolamento & purificação
RNA Ribossômico 16S/genética
Salinidade
Análise de Sequência de DNA
Solo/química
Vitamina K 2/análogos & derivados
Vitamina K 2/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Alkalies); 0 (DNA, Bacterial); 0 (Fatty Acids); 0 (Phospholipids); 0 (RNA, Ribosomal, 16S); 0 (Soil); 11032-49-8 (Vitamin K 2); 523-38-6 (vitamin MK 8)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180212
[Lr] Data última revisão:
180212
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180105
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.002548


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[PMID]:29337992
[Au] Autor:Emran KM; Al-Harbi AK
[Ad] Endereço:Chemistry Department, College of Science, Taibah University, Al-Madinah Al-Monawarah, Saudi Arabia.
[Ti] Título:Outstanding resistance and passivation behaviour of new Fe-Co metal-metal glassy alloys in alkaline media.
[So] Source:PLoS One;13(1):e0187567, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The electrochemical behavior of the oxide layers on two metal-metal glassy alloys, Fe78Co9Cr10Mo2Al1 (VX9)and Fe49Co49V2 (VX50) (at.%), were studied using electrochemical techniques including electrochemical frequency modulation (EFM), electrochemical impedance spectroscopy (EIS) and cyclic polarization (CP) measurements. The morphology and composition of the alloy surfaces were investigated using X-ray photoelectron spectroscopy (XPS), scanning electron microscopy (SEM) and atomic force microscopy (AFM). The corrosion rate and surface roughness of both alloys increased as the concentration of NaOH in aqueous solution was raised. The presence of some protective elements in the composition of the alloys led to the formation of a spontaneous passive layer on the alloy surface. The higher resistance values of both alloys were associated with the magnitude of the dielectric properties of the passive films formed on their surfaces. Both alloys are classified as having outstanding resistance to corrosion, which results from the formation of a passive film that acts as an efficient barrier to corrosion in alkaline solution.
[Mh] Termos MeSH primário: Álcalis/química
Ligas/química
Cobalto/química
Vidro
Ferro/química
[Mh] Termos MeSH secundário: Corrosão
Microscopia de Força Atômica
Microscopia Eletrônica de Varredura
Espectroscopia Fotoeletrônica
Propriedades de Superfície
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Alkalies); 0 (Alloys); 3G0H8C9362 (Cobalt); E1UOL152H7 (Iron)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180129
[Lr] Data última revisão:
180129
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180117
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0187567


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[PMID]:28743579
[Au] Autor:Zahedi S; Rivero M; Solera R; Perez M
[Ad] Endereço:Department of Environmental Technologies, University of Cadiz, Faculty of Marine and Environmental Sciences (CASEM), Pol. Río San Pedro s/n, 11510 Puerto Real (Cádiz), Spain. Electronic address: soraya.zahedi@uca.es.
[Ti] Título:Seeking to enhance the bioenergy of municipal sludge: Effect of alkali pre-treatment and soluble organic matter supplementation.
[So] Source:Waste Manag;68:398-404, 2017 Oct.
[Is] ISSN:1879-2456
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The aim of this research is to enhance the mesophilic anaerobic digestion of municipal sludge from Cadiz-San Fernando (Spain) wastewater treatment plant at 20days hydraulic retention time (HRT). Two different strategies were tested to improve the process: co-digestion with the addition of soluble organic matter (1% v/v); and alkali sludge pre-treatment (NaOH) prior to co-digestion with glycerine (1% v/v). Methane production (MP) was substantially enhanced (from 0.36±0.09 L CH l/d to 0.85±0.16 L CH l/d), as was specific methane production (SMP) (from 0.20±0.05 L CH /g VS to 0.49±0.09 L CH /g VS) when glycerine was added. The addition of glycerine does not seem to affect sludge stability, the quality of the effluent in terms of pH and organic matter content, i.e. volatile fatty acids (VFA), soluble organic matter and total volatile solid, or process stability (VFA/Alkalinity ratio<0.4). Alkali pre-treatment prior to co-digestion resulted in a high increase in soluble organic loading rates (more than 20%) and acidification yield (more than 50%). At 20days HRT, however, it led to overload of the system and total destabilization of the mesophilic anaerobic co-digestion of sewage sludge and glycerine.
[Mh] Termos MeSH primário: Reatores Biológicos
Esgotos
[Mh] Termos MeSH secundário: Álcalis
Anaerobiose
Metano
Espanha
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Alkalies); 0 (Sewage); OP0UW79H66 (Methane)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171213
[Lr] Data última revisão:
171213
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170727
[St] Status:MEDLINE


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[PMID]:28873608
[Au] Autor:Li T; Guo XN; Zhu KX; Zhou HM
[Ad] Endereço:State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, Jiangsu Province, PR China; School of Food Science and Technology, Jiangnan University, Wuxi 214122, Jiangsu Province, PR China.
[Ti] Título:Effects of alkali on protein polymerization and textural characteristics of textured wheat protein.
[So] Source:Food Chem;239:579-587, 2018 Jan 15.
[Is] ISSN:0308-8146
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The impact of alkali addition on the degree of gluten polymerization and textural characteristics of textured wheat protein was investigated. Results showed that the extrusion process increased the average molecular weight of gluten as evidenced by SDS-PAGE and SDS extractable protein. The addition of alkali not only promoted the degree of gluten polymerization, but also induced dehydroalanine-derived cross-linking. Alkali addition decreased the content of cystine and increased the contents of dehydroalanine and lanthionine. The obvious decrease of free SH showed that dehydroalanine-derived cross-linking was quantitatively less crucial than disulfide cross-linking. Furthermore, the protein cross-linking induced by alkali improved the texture properties of gluten extrudates. SEM analysis showed extrusion under alkaline condition conferred a more fibrous microstructure as a consequence of a compact gluten network.
[Mh] Termos MeSH primário: Triticum
[Mh] Termos MeSH secundário: Álcalis
Eletroforese em Gel de Poliacrilamida
Glutens
Proteínas de Plantas
Polimerização
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Alkalies); 0 (Plant Proteins); 8002-80-0 (Glutens)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171128
[Lr] Data última revisão:
171128
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170907
[St] Status:MEDLINE


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[PMID]:28976994
[Au] Autor:Guo M; Li S; Tian S; Wang B; Zhao X
[Ad] Endereço:College of Life Science, Luoyang Normal University, Luoyang, Henan, China.
[Ti] Título:Transcriptome analysis of genes involved in defense against alkaline stress in roots of wild jujube (Ziziphus acidojujuba).
[So] Source:PLoS One;12(10):e0185732, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Wild jujube (Ziziphus acidojujuba Mill.) is highly tolerant to alkaline, saline and drought stress; however, no studies have performed transcriptome profiling to study the response of wild jujube to these and other abiotic stresses. In this study, we examined the tolerance of wild jujube to NaHCO3-NaOH solution and analyzed gene expression profiles in response to alkaline stress. Physiological experiments revealed that H2O2 content in leaves increased significantly and root activity decreased quickly during alkaline of pH 9.5 treatment. For transcriptome analysis, wild jujube plants grown hydroponically were treated with NaHCO3-NaOH solution for 0, 1, and 12 h and six transcriptomes from roots were built. In total, 32,758 genes were generated, and 3,604 differentially expressed genes (DEGs) were identified. After 1 h, 853 genes showed significantly different expression between control and treated plants; after 12 h, expression of 2,856 genes was significantly different. The expression pattern of nine genes was validated by quantitative real-time PCR. After gene annotation and gene ontology enrichment analysis, the genes encoding transcriptional factors, serine/threonine-protein kinases, heat shock proteins, cysteine-like kinases, calmodulin-like proteins, and reactive oxygen species (ROS) scavengers were found to be closely involved in alkaline stress response. These results will provide useful insights for elucidating the mechanisms underlying alkaline tolerance in wild jujube.
[Mh] Termos MeSH primário: Álcalis/farmacologia
Genes de Plantas
Raízes de Plantas/genética
Transcriptoma
Ziziphus/genética
[Mh] Termos MeSH secundário: Regulação da Expressão Gênica de Plantas
Reação em Cadeia da Polimerase em Tempo Real
Análise de Sequência de RNA
[Pt] Tipo de publicação:JOURNAL ARTICLE; VALIDATION STUDIES
[Nm] Nome de substância:
0 (Alkalies)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171102
[Lr] Data última revisão:
171102
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171005
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0185732


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[PMID]:28957378
[Au] Autor:Watson SA; Fabricius KE; Munday PL
[Ad] Endereço:Australian Research Council Centre of Excellence for Coral Reef Studies, James Cook University, Townsville, Queensland, Australia.
[Ti] Título:Quantifying pCO2 in biological ocean acidification experiments: A comparison of four methods.
[So] Source:PLoS One;12(9):e0185469, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Quantifying the amount of carbon dioxide (CO2) in seawater is an essential component of ocean acidification research; however, equipment for measuring CO2 directly can be costly and involve complex, bulky apparatus. Consequently, other parameters of the carbonate system, such as pH and total alkalinity (AT), are often measured and used to calculate the partial pressure of CO2 (pCO2) in seawater, especially in biological CO2-manipulation studies, including large ecological experiments and those conducted at field sites. Here we compare four methods of pCO2 determination that have been used in biological ocean acidification experiments: 1) Versatile INstrument for the Determination of Total inorganic carbon and titration Alkalinity (VINDTA) measurement of dissolved inorganic carbon (CT) and AT, 2) spectrophotometric measurement of pHT and AT, 3) electrode measurement of pHNBS and AT, and 4) the direct measurement of CO2 using a portable CO2 equilibrator with a non-dispersive infrared (NDIR) gas analyser. In this study, we found these four methods can produce very similar pCO2 estimates, and the three methods often suited to field-based application (spectrophotometric pHT, electrode pHNBS and CO2 equilibrator) produced estimated measurement uncertainties of 3.5-4.6% for pCO2. Importantly, we are not advocating the replacement of established methods to measure seawater carbonate chemistry, particularly for high-accuracy quantification of carbonate parameters in seawater such as open ocean chemistry, for real-time measures of ocean change, nor for the measurement of small changes in seawater pCO2. However, for biological CO2-manipulation experiments measuring differences of over 100 µatm pCO2 among treatments, we find the four methods described here can produce similar results with careful use.
[Mh] Termos MeSH primário: Ácidos/química
Dióxido de Carbono/análise
Biologia Marinha/métodos
Oceanos e Mares
Pressão Parcial
[Mh] Termos MeSH secundário: Álcalis/química
Eletrodos
Raios Infravermelhos
Padrões de Referência
Água do Mar/química
Espectrofotometria
Incerteza
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Acids); 0 (Alkalies); 142M471B3J (Carbon Dioxide)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171018
[Lr] Data última revisão:
171018
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170929
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0185469


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[PMID]:28920839
[Au] Autor:Sorokin DY; Khijniak TV; Galinski EA; Kublanov IV
[Ad] Endereço:1​Winogradsky Institute of Microbiology, Research Centre of Biotechnology, Russian Academy of Sciences, Moscow, Russia.
[Ti] Título:Natronotalea proteinilytica gen. nov., sp. nov. and Longimonas haloalkaliphila sp. nov., extremely haloalkaliphilic members of the phylum Rhodothermaeota from hypersaline alkaline lakes.
[So] Source:Int J Syst Evol Microbiol;67(10):4161-4167, 2017 Oct.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Two proteolytic bacterial strains, BSker2 and BSker3 , were enriched from sediments of hypersaline alkaline lakes in Kulunda Steppe (Altai, Russia) with chicken feathers as substrate, followed by pure culture isolation on hypersaline alkaline media with casein. The cells were non-motile, filamentous, flexible rods. The isolates were obligately aerobic heterotrophs utilizing proteins and peptides as growth substrates. Both were obligate alkaliphiles, but differed in their pH optimum for growth: pH 9.5-9.8 for Bsker2 and pH 8.5-8.8 for BSker3 . The salt range for growth of both isolates was between 2 and 4.5 M total Na with an optimum at 2.5-3 M. No organic osmolytes were detected in cells of BSker2 , but they accumulated high intracellular concentrations of K . The polar lipid fatty acids were dominated by unsaturated C16 and C18 species. The 16S rRNA gene phylogeny indicated that both strains belong to the recently proposed phylum Rhodothermaeota. BSker2 forms a novel genus-level branch, while BSker3 represents a novel species-level member in the genus Longimonas. On the basis of distinct phenotypic and genotypic properties, strain BSker2 (=JCM 31342 =UNIQEM U1009 ) is proposed to be classified as a representative of a novel genus and species, Natronotalea proteinilyticagen. nov., sp. nov., and strain BSker3 (=JCM 31343 =UNIQEM U1010 ) as a representative of a novel species, Longimonas haloalkaliphila sp. nov.
[Mh] Termos MeSH primário: Álcalis
Bacteroidetes/classificação
Lagos/microbiologia
Filogenia
Salinidade
[Mh] Termos MeSH secundário: Técnicas de Tipagem Bacteriana
Bacteroidetes/genética
Bacteroidetes/isolamento & purificação
Composição de Bases
DNA Bacteriano/genética
Ácidos Graxos/química
Concentração de Íons de Hidrogênio
RNA Ribossômico 16S/genética
Federação Russa
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Alkalies); 0 (DNA, Bacterial); 0 (Fatty Acids); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171103
[Lr] Data última revisão:
171103
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170919
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.002272


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[PMID]:28905704
[Au] Autor:Borsodi AK; Szili-Kovács T; Schumann P; Spröer C; Márialigeti K; Tóth E
[Ad] Endereço:1​Department of Microbiology, ELTE Eötvös Loránd University, Pázmány P. sétány 1/C, H-1117 Budapest, Hungary.
[Ti] Título:Nesterenkonia pannonica sp. nov., a novel alkaliphilic and moderately halophilic actinobacterium.
[So] Source:Int J Syst Evol Microbiol;67(10):4116-4120, 2017 Oct.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:An alkaliphilic and moderately halophilic bacterial strain characterized by optimal growth at pH 9.0-10.0 and with 5-7 % (w/v) NaCl, designated BV-35 , was isolated from water of a soda pan located in Kiskunság National Park, Hungary. Cells of the orange-pigmented colony were Gram-stain-positive, non-motile and non-endospore-forming coccoid rods. The isolate was strictly aerobic, catalase-positive and oxidase-negative. Strain BV-35 displayed a peptidoglycan similar to type A4α, l-Lys-l-Glu (A11.54 according to www.peptidoglycan-types.info) but containing additionally 4-aminobutyric acid. Menaquinone-7 (MK-7) was the predominant isoprenoid quinone, and anteiso-C15 : 0 and anteiso-C17 : 0 were its major cellular fatty acids. The DNA G+C content of strain BV-35 was 65.4 mol%. Based on 16S rRNA gene sequence similarities, the novel isolate showed the closest relationship to Nesterenkonia populi GP 10-3 (97.9 %). The DNA-DNA relatedness between BV-35 and N. populi was 46.7 %. The distinguishing phenotypic and genetic results of this polyphasic study revealed that strain BV-35 represents a novel member of the genus Nesterenkonia, for which the name Nesterenkonia pannonica sp. nov. is proposed. The type strain is BV-35 (=DSM 29786 =NCAIM B 02606 ).
[Mh] Termos MeSH primário: Micrococcaceae/classificação
Filogenia
Microbiologia da Água
[Mh] Termos MeSH secundário: Álcalis
Técnicas de Tipagem Bacteriana
Composição de Bases
DNA Bacteriano/genética
Ácidos Graxos/química
Hungria
Concentração de Íons de Hidrogênio
Micrococcaceae/genética
Micrococcaceae/isolamento & purificação
Hibridização de Ácido Nucleico
Peptidoglicano/química
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
Vitamina K 2/análogos & derivados
Vitamina K 2/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Alkalies); 0 (DNA, Bacterial); 0 (Fatty Acids); 0 (Peptidoglycan); 0 (RNA, Ribosomal, 16S); 11032-49-8 (Vitamin K 2); 8427BML8NY (vitamin MK 7)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171103
[Lr] Data última revisão:
171103
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170915
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.002263


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[PMID]:28884328
[Au] Autor:Cao L; Yu Y; Ding X; Zhu D; Yang F; Liu B; Sun X; Duan X; Yin K; Zhu Y
[Ad] Endereço:Key Laboratory of Agricultural Biological Functional Genes, Northeast Agricultural University, Harbin, 150030, People's Republic of China.
[Ti] Título:The Glycine soja NAC transcription factor GsNAC019 mediates the regulation of plant alkaline tolerance and ABA sensitivity.
[So] Source:Plant Mol Biol;95(3):253-268, 2017 Oct.
[Is] ISSN:1573-5028
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:KEY MESSAGE: Overexpression of Gshdz4 or GsNAC019 enhanced alkaline tolerance in transgenic Arabidopsis. We proved that Gshdz4 up-regulated both GsNAC019 and GsRD29B but GsNAC019 may repress the GsRD29B expression under alkaline stress. Wild soybean (Glycine soja) has a high tolerance to environmental challenges. It is a model species for dissecting the molecular mechanisms of salt-alkaline stresses. Although many NAC transcription factors play important roles in response to multiple abiotic stresses, such as salt, osmotic and cold, their mode of action in alkaline stress resistance is largely unknown. In our study, we identified a G. soja NAC gene, GsNAC019, which is a homolog of the Arabidopsis AtNAC019 gene. GsNAC019 was highly up-regulated by 50 mM NaHCO treatment in the roots of wild soybean. Further investigation showed that a well-characterized transcription factor, Gshdz4 protein, bound the cis-acting element sequences (CAATA/TA), which are located in the promoter of the AtNAC019/GsNAC019 genes. Overexpression of Gshdz4 positively regulated AtNAC019 expression in transgenic Arabidopsis, implying that AtNAC019/GsNAC019 may be the target genes of Gshdz4. GsNAC019 was demonstrated to be a nuclear-localized protein in onion epidermal cells and possessed transactivation activity in yeast cells. Moreover, overexpression of GsNAC019 in Arabidopsis resulted in enhanced tolerance to alkaline stress at the seedling and mature stages, but reduced ABA sensitivity. The closest Arabidopsis homolog mutant plants of Gshdz4, GsNAC019 and GsRD29B containing athb40, atnac019 and atrd29b were sensitive to alkaline stress. Overexpression or the closest Arabidopsis homolog mutant plants of the GsNAC019 gene in Arabidopsis positively or negatively regulated the expression of stress-related genes, such as AHA2, RD29A/B and KIN1. Moreover, this mutation could phenotypically promoted or compromised plant growth under alkaline stress, implying that GsNAC019 may contribute to alkaline stress tolerance via the ABA signal transduction pathway and regulate expression of the downstream stress-related genes.
[Mh] Termos MeSH primário: Ácido Abscísico/farmacologia
Álcalis/farmacologia
Fabaceae/genética
Regulação da Expressão Gênica de Plantas/efeitos dos fármacos
Proteínas de Plantas/genética
Fatores de Transcrição/genética
[Mh] Termos MeSH secundário: Adaptação Fisiológica/efeitos dos fármacos
Adaptação Fisiológica/genética
Arabidopsis/genética
Arabidopsis/metabolismo
Sequência de Bases
Bicarbonatos/farmacologia
Fabaceae/metabolismo
Reguladores de Crescimento de Planta/farmacologia
Proteínas de Plantas/metabolismo
Plantas Geneticamente Modificadas
Regiões Promotoras Genéticas/genética
Ligação Proteica
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Homologia de Sequência do Ácido Nucleico
Cloreto de Sódio/farmacologia
Estresse Fisiológico
Fatores de Transcrição/metabolismo
Técnicas do Sistema de Duplo-Híbrido
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Alkalies); 0 (Bicarbonates); 0 (Plant Growth Regulators); 0 (Plant Proteins); 0 (Transcription Factors); 451W47IQ8X (Sodium Chloride); 72S9A8J5GW (Abscisic Acid)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171030
[Lr] Data última revisão:
171030
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170909
[St] Status:MEDLINE
[do] DOI:10.1007/s11103-017-0643-3



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