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  1 / 130487 MEDLINE  
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[PMID]:29447189
[Au] Autor:Saleem AR; Brunetti C; Khalid A; Della Rocca G; Raio A; Emiliani G; De Carlo A; Mahmood T; Centritto M
[Ad] Endereço:Department of Earth and Environmental Sciences, Bahria University Islamabad Campus, Islamabad, Pakistan.
[Ti] Título:Drought response of Mucuna pruriens (L.) DC. inoculated with ACC deaminase and IAA producing rhizobacteria.
[So] Source:PLoS One;13(2):e0191218, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Drought is one of the major constraints limiting agricultural production worldwide and is expected to increase in the future. Limited water availability causes significant effects to plant growth and physiology. Plants have evolved different traits to mitigate the stress imposed by drought. The presence of plant growth-promoting rhizobacteria (PGPR) could play an important role in improving plant performances and productivity under drought. These beneficial microorganisms colonize the rhizosphere of plants and increase drought tolerance by lowering ethylene formation. In the present study, we demonstrate the potential to improve the growth of velvet bean under water deficit conditions of two different strains of PGPR with ACCd (1-Aminocyclopropane-1-Carboxylate deaminase) activity isolated from rainfed farming system. We compared uninoculated and inoculated plants with PGPR to assess: a) photosynthetic performance and biomass; b) ACC content and ethylene emission from leaves and roots; c) leaf isoprene emission. Our results provided evidence that under drought conditions inoculation with PGPR containing the ACCd enzyme could improve plant growth compared to untreated plants. Ethylene emission from roots and leaves of inoculated velvet bean plants was significantly lower than uninoculated plants. Moreover, isoprene emission increased with drought stress progression and was higher in inoculated plants compared to uninoculated counterparts. These findings clearly illustrate that selected PGPR strains isolated from rainfed areas could be highly effective in promoting plant growth under drought conditions by decreasing ACC and ethylene levels in plants.
[Mh] Termos MeSH primário: Mucuna/metabolismo
Reguladores de Crescimento de Planta/metabolismo
Rhizobiaceae/metabolismo
[Mh] Termos MeSH secundário: Biomassa
Carbono-Carbono Liases/metabolismo
Secas
Etilenos/biossíntese
Mucuna/fisiologia
Fotossíntese
Desenvolvimento Vegetal
Reguladores de Crescimento de Planta/fisiologia
Raízes de Plantas/crescimento & desenvolvimento
Rhizobiaceae/genética
Rizosfera
Microbiologia do Solo
Água/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Ethylenes); 0 (Plant Growth Regulators); 059QF0KO0R (Water); 91GW059KN7 (ethylene); EC 3.5.99.7 (1-aminocyclopropane-1-carboxylate deaminase); EC 4.1.- (Carbon-Carbon Lyases)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180216
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0191218


  2 / 130487 MEDLINE  
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[PMID]:29377914
[Au] Autor:Grasso R; Musumeci F; Gulino M; Scordino A
[Ad] Endereço:Department of Physics and Astronomy, Catania University, Catania, Italy.
[Ti] Título:Exploring the behaviour of water in glycerol solutions by using delayed luminescence.
[So] Source:PLoS One;13(1):e0191861, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The crucial role of water in the engine of life have encouraged many researchers in studying, both theoretically and experimentally, the possible "structure" of water. Many properties of water have been related to the interplay between two distinct and interconverting structural species, namely the low-density water (LDW) and the high-density water (HDW). Supported by the results obtained with other aqueous solutions, this paper deals with the possibility of using the ultra-weak delayed luminescence (DL) to investigate water structuring in a mixture with glycerol, characterized only by hydrogen bonds between the various molecules. Spectral and temporal characteristics of DL decays give information on the two components of the mixture, by evidencing the contribution of water at glycerol concentrations close to the values used in cryopreservation. DL results have shown a correlation with LDW clusters size as determined by other researchers on the basis of neutron diffraction experiments and computational modelling, as reported in Literature.
[Mh] Termos MeSH primário: Glicerol/química
Água/química
[Mh] Termos MeSH secundário: Luminescência
Soluções
Análise Espectral
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Solutions); 059QF0KO0R (Water); PDC6A3C0OX (Glycerol)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180130
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0191861


  3 / 130487 MEDLINE  
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[PMID]:29367490
[Au] Autor:Asakawa T; Takano Y; Ohta A; Asakawa H
[Ad] Endereço:School of Chemistry, College of Science and Engineering, Kanazawa University.
[Ti] Título:Aggregation and pH Responsive Behavior of Thioester Surfactants and Formation of Disulfide Linkages in Aqueous Solutions.
[So] Source:J Oleo Sci;67(2):199-206, 2018 Feb 01.
[Is] ISSN:1347-3352
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:pH responsive surfactants, [C H N(CH ) (CH ) SCOCH ]Br (C nSAc, n = 4, 11, 12), were prepared, and their properties in aqueous solution were examined. The critical micelle concentration (cmc) and critical vesicle concentration (cvc) were determined based on changes in conductivity, as well as by fluorescence measurements, and light scattering methods. A significant increase in the light scattering intensities of the C nSAc (n=11, 12) systems suggested that the growth of aggregates was accompanied by considerable counterion binding with increasing surfactant concentration. The diameter of C 11SAc, recorded by the dynamic light scattering measurements, was about 9.6 ±1.0 nm, which was slightly smaller than that for didodecyldimethylammonium bromide (DDAB) vesicles. The thioester group was easily hydrolyzed upon the addition of NaOH, while it was hardly hydrolyzed with the addition of HCl. The time course of alkaline hydrolysis was examined by the conductivity measurements and high-performance liquid chromatography analysis. [C H N(CH ) (CH ) SS(CH ) N(CH ) C H ]2Br (2C 11SS) was generated in the C 11SAc alkaline solution because of air oxidation. The C 11SAc alkaline solution gradually became an opaque blue color with increasing light scattering at 346 nm, indicating the remarkable growth of vesicles. The chemical structure of 2C 11SS was consistent with that of a disulfide linked double tailed surfactant, similar to DDAB. The disulfide linkage between the double tailed surfactants will contribute to the stabilization and growth of vesicles.
[Mh] Termos MeSH primário: Ésteres/química
Tensoativos/química
Água/química
[Mh] Termos MeSH secundário: Dissulfetos/química
Difusão Dinâmica da Luz
Concentração de Íons de Hidrogênio
Hidrólise
Micelas
Oxirredução
Compostos de Amônio Quaternário/química
Soluções
Tensão Superficial
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Disulfides); 0 (Esters); 0 (Micelles); 0 (Quaternary Ammonium Compounds); 0 (Solutions); 0 (Surface-Active Agents); 059QF0KO0R (Water); 13146-86-6 (didodecyldimethylammonium)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180126
[St] Status:MEDLINE
[do] DOI:10.5650/jos.ess17192


  4 / 130487 MEDLINE  
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[PMID]:29367483
[Au] Autor:Matsuura T; Ogawa A; Ohara Y; Nishina S; Nakanishi M; Gohtani S
[Ad] Endereço:Department of Applied Biological Science, Kagawa University.
[Ti] Título:Effect of Alcohols on the Phase Behavior and Emulsification of a Sucrose Fatty Acid Ester/Water/Edible Oil System.
[So] Source:J Oleo Sci;67(2):167-176, 2018 Feb 01.
[Is] ISSN:1347-3352
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:The effect of alcohols (ethanol, 1-propanol, propylene glycol, glycerin, sucrose) on the phase behavior and emulsification of sucrose stearic acid ester (SSE)/water/edible vegetable oil (EVO) systems was investigated. Adding sucrose, propylene glycol, and glycerin narrowed the oil-separated two-phase region in the phase diagram of the SSE/water/EVO systems, whereas adding ethanol and 1-propanol expanded the oil-separated two-phase region. Changing the course of emulsification in the phase diagram showed that the size of the oil-droplet particle typically decreased in a system with a narrowed oil-separated region. The emulsification properties of the systems varied with respect to changes in the phase diagram. The microstructure of the systems was examined using small-angle X-ray scattering, and the ability to retain the oil in the lamellar structure of the SSEs was suggested as an important role in emulsification, because the mechanism of the systems was the same as that for the liquid crystal emulsification method.
[Mh] Termos MeSH primário: Álcoois/química
Ésteres/química
Ácidos Graxos/química
Transição de Fase
Óleos Vegetais/química
Ácidos Esteáricos/química
Sacarose/química
Água/química
[Mh] Termos MeSH secundário: Emulsões
Glicerol/química
Propilenoglicol/química
Espalhamento a Baixo Ângulo
Difração de Raios X
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Alcohols); 0 (Emulsions); 0 (Esters); 0 (Fatty Acids); 0 (Plant Oils); 0 (Stearic Acids); 059QF0KO0R (Water); 4ELV7Z65AP (stearic acid); 57-50-1 (Sucrose); 6DC9Q167V3 (Propylene Glycol); PDC6A3C0OX (Glycerol)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180126
[St] Status:MEDLINE
[do] DOI:10.5650/jos.ess17146


  5 / 130487 MEDLINE  
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[PMID]:29367478
[Au] Autor:Sun M; Xu X; Zhang Q; Rui X; Wu J; Dong M
[Ad] Endereço:College of Food Science and Technology, Nanjing Agricultural University.
[Ti] Título:Ultrasonic-assisted Aqueous Extraction and Physicochemical Characterization of Oil from Clanis bilineata.
[So] Source:J Oleo Sci;67(2):151-165, 2018 Feb 01.
[Is] ISSN:1347-3352
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:Ultrasound-assisted aqueous extraction (UAAE) was used to extract oil from Clanis bilineata (CB), a traditional edible insect that can be reared on a large scale in China, and the physicochemical property and antioxidant capacity of the UAAE-derived oil (UAAEO) were investigated for the first time. UAAE conditions of CB oil was optimized using response surface methodology (RSM) and the highest oil yield (19.47%) was obtained under optimal conditions for ultrasonic power, extraction temperature, extraction time, and ultrasonic interval time at 400 W, 40°C, 50 min, and 2 s, respectively. Compared with Soxhlet extraction-derived oil (SEO), UAAEO had lower acid (AV), peroxide (PV) and p-anisidine values (PAV) as well as higher polyunsaturated fatty acids contents and thermal stability. Furthermore, UAAEO showed stronger antioxidant activities than those of SEO, according to DPPH radical scavenging and ß-carotene bleaching tests. Therefore, UAAE is a promising process for the large-scale production of CB oil and CB has a developing potential as functional oil resource.
[Mh] Termos MeSH primário: Fenômenos Químicos
Larva/química
Lepidópteros/química
Extração Líquido-Líquido/métodos
Óleos/química
Óleos/isolamento & purificação
Ultrassom
Água
[Mh] Termos MeSH secundário: Animais
Antioxidantes
Ácidos Graxos Insaturados/análise
Óleos/farmacologia
Temperatura Ambiente
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antioxidants); 0 (Fatty Acids, Unsaturated); 0 (Oils); 059QF0KO0R (Water)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180126
[St] Status:MEDLINE
[do] DOI:10.5650/jos.ess17108


  6 / 130487 MEDLINE  
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[PMID]:29364942
[Au] Autor:Yuan X; Wen B
[Ad] Endereço:Center for Integrative Conservation, Xishuangbanna Tropical Botanical Garden, the Chinese Academy of Sciences, Mengla, Yunnan, China.
[Ti] Título:Seed germination response to high temperature and water stress in three invasive Asteraceae weeds from Xishuangbanna, SW China.
[So] Source:PLoS One;13(1):e0191710, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Crassocephalum crepidioides, Conyza canadensis, and Ageratum conyzoides are alien annuals naturalized in China, which produce a large number of viable seeds every year. They widely grow in Xishuangbanna, becoming troublesome weeds that compete with crops for water and nutrients. As seed germination is among the most important life-stages which contribute to plant distribution and invasiveness, its adaptation to temperature and water stress were investigated in these three species. Results showed that: (1) These three species have wide temperature ranges to allow seed germination, i.e., high germination and seedling percentages were achieved between 15°C and 30°C, but germination was seriously inhibited at 35°C; only A. conyzoides demonstrated relative preference for warmer temperatures with approximately 25% germination and seedling percentage at 35°C; (2) light was a vital germination prerequisite for C. crepidioides and A. conyzoides, whereas most C. canadensis seeds germinated in full darkness; (3) Although all three species have good adaptation to bare ground habitat characterized by high temperatures and water stress, including their tolerance to soil surface temperatures of 70°C in air-dried seeds, A. conyzoides seeds exhibited higher tolerance to both continuous and daily periodic high-temperature treatment at 40°C, and to water restriction (e.g., ca. 65% seeds germinated to -0.8 MPa created by NaCl), which is consistent with their field behavior in Xishuangbanna. This study suggests that seed high-temperature tolerance contributes to the weed attributes of these three species, and that adaptation to local micro-habitats is a critical determinant for invasiveness of an alien plant.
[Mh] Termos MeSH primário: Asteraceae/fisiologia
Germinação
Temperatura Alta
Plantas Daninhas/fisiologia
Sementes/fisiologia
Estresse Fisiológico
Água
[Mh] Termos MeSH secundário: Asteraceae/embriologia
China
Espécies Introduzidas
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
059QF0KO0R (Water)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180125
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0191710


  7 / 130487 MEDLINE  
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[PMID]:29353043
[Au] Autor:Tanaka M; Ishihara Y; Mizuno S; Ishida A; Vogel CF; Tsuji M; Yamazaki T; Itoh K
[Ad] Endereço:Laboratory of Molecular Brain Science, Graduate School of Integrated Arts and Sciences, Hiroshima University, Hiroshima, 739-8521, Japan; Laboratory for Pharmacotherapy and Experimental Neurology, Kagawa School of Pharmaceutical Sciences, Tokushima Bunri University, Kagawa, 769-2193, Japan.
[Ti] Título:Progression of vasogenic edema induced by activated microglia under permanent middle cerebral artery occlusion.
[So] Source:Biochem Biophys Res Commun;496(2):582-587, 2018 02 05.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Brain edema is a severe complication that accompanies ischemic stroke. Increasing evidence shows that inflammatory cytokines impair tight junctions of the blood-brain barrier, suggesting the involvement of microglia in brain edema. In this study, we examined the role of microglia in the progression of ischemic brain edema using mice with permanent middle cerebral artery occlusion. The intensity of T2-weighted imaging (T2WI) in the cerebral cortex and the striatum was elevated 3 h after occlusion and spread to peripheral regions of the ischemic hemisphere. Merged images of 2,3,5-triphenyl tetrazolium chloride staining and T2WI revealed the exact vasogenic edema region, which spread from the ischemic core to outside the ischemic region. Microglia were strongly activated in the ischemic region 3 h after occlusion and, notably, activated microglia were observed in the non-ischemic region 24 h after occlusion. Pretreatment with minocycline, an inhibitor of microglial activation clearly suppressed not only vasogenic edema but also infarct formation. We demonstrated in this study that vasogenic edema spreads from the ischemic core to the peripheral region, which can be elicited, at least in part, by microglial activation induced by ischemia.
[Mh] Termos MeSH primário: Edema Encefálico/etiologia
Encéfalo/patologia
Infarto da Artéria Cerebral Média/complicações
Microglia/patologia
[Mh] Termos MeSH secundário: Animais
Edema Encefálico/patologia
Progressão da Doença
Infarto da Artéria Cerebral Média/patologia
Masculino
Camundongos Endogâmicos ICR
Água/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
059QF0KO0R (Water)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180122
[St] Status:MEDLINE


  8 / 130487 MEDLINE  
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[PMID]:29264676
[Au] Autor:Faraji H; Helalizadeh M; Kordi MR
[Ad] Endereço:Department of Chemistry, Varamin-Pishva Branch, Islamic Azad University, Varamin, 338177489, Iran. hakimfaraji@yahoo.com.
[Ti] Título:Overcoming the challenges of conventional dispersive liquid-liquid microextraction: analysis of THMs in chlorinated swimming pools.
[So] Source:Anal Bioanal Chem;410(2):605-614, 2018 Jan.
[Is] ISSN:1618-2650
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:A rapid, simple, and sensitive approach to the analysis of trihalomethanes (THMs) in swimming pool water samples has been developed. The main goal of this study was to overcome or to improve the shortcomings of conventional dispersive liquid-liquid microextraction (DLLME) and to maximize the realization of green analytical chemistry principles. The method involves a simple vortex-assisted microextraction step, in the absence of the dispersive solvent, followed by salting-out effect for the elimination of the centrifugation step. A bell-shaped device and a solidifiable solvent were used to simplify the extraction solvent collection after phase separation. Optimization of the independent variables was performed by using chemometric methods in three steps. The method was statistically validated based on authentic guidance documents. The completion time for extraction was less than 8 min, and the limits of detection were in the range between 4 and 72 ng L . Using this method, good linearity and precision were achieved. The results of THMs determination in different real samples showed that in some cases the concentration of total THMs was more than threshold values of THMs determined by accredited healthcare organizations. This method indicated satisfactory analytical figures of merit. Graphical Abstract A novel green microextraction technique for overcoming the challenges of conventional DLLME. The proposed procedure complies with the principles of green/sustainable analytical chemistry, comprising decreasing the sample size, making easy automation of the process, reducing organic waste, diminishing energy consumption, replacing toxic reagents with safer reagents, and enhancing operator safety.
[Mh] Termos MeSH primário: Microextração em Fase Líquida/métodos
Piscinas
Trialometanos/análise
Poluentes Químicos da Água/análise
[Mh] Termos MeSH secundário: Halogenação
Limite de Detecção
Solventes
Trialometanos/isolamento & purificação
Água/análise
Poluentes Químicos da Água/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE; VALIDATION STUDIES
[Nm] Nome de substância:
0 (Solvents); 0 (Trihalomethanes); 0 (Water Pollutants, Chemical); 059QF0KO0R (Water)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171222
[St] Status:MEDLINE
[do] DOI:10.1007/s00216-017-0762-3


  9 / 130487 MEDLINE  
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[PMID]:29214538
[Au] Autor:Akbarzade S; Chamsaz M; Rounaghi GH; Ghorbani M
[Ad] Endereço:Department of Chemistry, Faculty of Sciences, Ferdowsi University of Mashhad, Mashhad, Iran.
[Ti] Título:Zero valent Fe-reduced graphene oxide quantum dots as a novel magnetic dispersive solid phase microextraction sorbent for extraction of organophosphorus pesticides in real water and fruit juice samples prior to analysis by gas chromatography-mass spectrometry.
[So] Source:Anal Bioanal Chem;410(2):429-439, 2018 Jan.
[Is] ISSN:1618-2650
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:A selective and sensitive magnetic dispersive solid-phase microextraction (MDSPME) coupled with gas chromatography-mass spectrometry was developed for extraction and determination of organophosphorus pesticides (Sevin, Fenitrothion, Malathion, Parathion, and Diazinon) in fruit juice and real water samples. Zero valent Fe-reduced graphene oxide quantum dots (rGOQDs@ Fe) as a new and effective sorbent were prepared and applied for extraction of organophosphorus pesticides using MDSPME method. In order to study the performance of this new sorbent, the ability of rGOQDs@ Fe was compared with graphene oxide and magnetic graphene oxide nanocomposite by recovery experiments of the organophosphorus pesticides. Several affecting parameters in the microextraction procedure, including pH of donor phase, donor phase volume, stirring rate, extraction time, and desorption conditions such as the type and volume of solvents and desorption time were thoroughly investigated and optimized. Under the optimal conditions, the method showed a wide linear dynamic range with R-square between 0.9959 and 0.9991. The limit of detections, the intraday and interday relative standard deviations (n = 5) were less than 0.07 ngmL , 4.7, and 8.6%, respectively. The method was successfully applied for extraction and determination of organophosphorus pesticides in real water samples (well, river and tap water) and fruit juice samples (apple and grape juice). The obtained relative recoveries were in the range of 82.9%-113.2% with RSD percentages of less than 5.8% for all the real samples.
[Mh] Termos MeSH primário: Sucos de Frutas e Vegetais/análise
Grafite/química
Compostos Organofosforados/análise
Praguicidas/análise
Microextração em Fase Sólida/métodos
Poluentes Químicos da Água/análise
Água/análise
[Mh] Termos MeSH secundário: Cromatografia Gasosa-Espectrometria de Massas/métodos
Ferro/química
Limite de Detecção
Compostos Organofosforados/isolamento & purificação
Oxirredução
Óxidos/química
Praguicidas/isolamento & purificação
Pontos Quânticos/química
Poluentes Químicos da Água/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE; VALIDATION STUDIES
[Nm] Nome de substância:
0 (Organophosphorus Compounds); 0 (Oxides); 0 (Pesticides); 0 (Water Pollutants, Chemical); 059QF0KO0R (Water); 7782-42-5 (Graphite); E1UOL152H7 (Iron)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171208
[St] Status:MEDLINE
[do] DOI:10.1007/s00216-017-0732-9


  10 / 130487 MEDLINE  
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[PMID]:28462910
[Au] Autor:Mihailescu IN; Bociaga D; Popescu-Pelin G; Stan GE; Duta L; Socol G; Chifiriuc MC; Bleotu C; Lazar V; Husanu MA; Zgura I; Miculescu F; Negut I; Hapenciuc C
[Ad] Endereço:National Institute for Lasers, Plasma and Radiation Physics, 077125 Magurele, Romania.
[Ti] Título:Optimized silicon reinforcement of carbon coatings by pulsed laser technique for superior functional biomedical surfaces fabrication.
[So] Source:Biofabrication;9(2):025029, 2017 Jun 01.
[Is] ISSN:1758-5090
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:We report on the fabrication of silicon-reinforced carbon (C:Si) structures by combinatorial pulsed laser deposition to search for the best design for a new generation of multi-functional coated implants. The synthesized films were characterized from the morphological, structural, compositional, mechanical and microbiological points of view. Scanning electron microscopy revealed the presence, on top of the deposited layers, of spheroid particulates with sizes in the micron range. No micro-cracks or delaminations were observed. Energy dispersive x-ray spectroscopy and grazing incidence x-ray diffraction pointed to the existence of a C to Si compositional gradient from one end of the film to the other. Raman investigation revealed a relatively high sp hybridization of up to 80% at 40-48 mm apart from the edge with higher C content. Si addition was demonstrated to significantly increase C:Si film bonding to the substrate, with values above the ISO threshold for coatings to be used in high-loading biomedical applications. Surface energy studies pointed to an increase in the hydrophilic character of the deposited structures along with Si content up to 52 mN m . In certain cases, the Si-reinforced C coatings elicited an antimicrobial biofilm action. The presence of Si was proven to be benign to HEp-2 cells of human origin, without interfering with their cellular cycle. On this basis, reliable C:Si structures with good adherence to the substrate and high efficiency against microbial biofilms can be developed for implant coatings and other advanced medical devices.
[Mh] Termos MeSH primário: Tecnologia Biomédica/métodos
Carbono/química
Materiais Revestidos Biocompatíveis/química
Lasers
Silício/química
[Mh] Termos MeSH secundário: Ciclo Celular
Forma Celular
Seres Humanos
Teste de Materiais
Testes de Sensibilidade Microbiana
Microscopia Eletrônica de Varredura
Espectrometria por Raios X
Análise Espectral Raman
Propriedades de Superfície
Água/química
Difração de Raios X
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Coated Materials, Biocompatible); 059QF0KO0R (Water); 7440-44-0 (Carbon); Z4152N8IUI (Silicon)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170503
[St] Status:MEDLINE
[do] DOI:10.1088/1758-5090/aa7076



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