[PMID]: | 28450175 |
[Au] Autor: | Madamet M; Kounta MB; Wade KA; Lo G; Diawara S; Fall M; Bercion R; Nakoulima A; Fall KB; Benoit N; Gueye MW; Fall B; Diatta B; Pradines B |
[Ad] Endereço: | Unité de parasitologie et d'entomologie, Département des maladies infectieuses, Institut de recherche biomédicale des armées, Marseille, France; Aix-Marseille Université, Unité de recherche sur les maladies infectieuses et tropicales emergentes (URMITE), UM 63, CNRS 7278, IRD 198, Inserm 1095, Marse |
[Ti] Título: | Absence of association between polymorphisms in the K13 gene and the presence of Plasmodium falciparum parasites at day 3 after treatment with artemisinin derivatives in Senegal. |
[So] Source: | Int J Antimicrob Agents;49(6):754-756, 2017 Jun. |
[Is] ISSN: | 1872-7913 |
[Cp] País de publicação: | Netherlands |
[La] Idioma: | eng |
[Ab] Resumo: | In 2006, the Senegalese National Malaria Control Programme recommended artemisinin-based combination therapy as first-line treatment for uncomplicated malaria. In addition, intravenous (i.v.) injection of artesunate and artemether has gradually replaced quinine for the treatment of severe malaria. Mutations in the propeller domain of the Kelch 13 gene (K13-propeller, PF3D71343700), such as Y493H, R539T, I543T and C580Y, were recently associated with in vivo and in vitro resistance to artemisinin in Southeast Asia. However, these mutations were not identified in Africa. In total, 181 isolates of Plasmodium falciparum from 161 patients from Dakar, Senegal, were collected between August 2015 and January 2016. The K13-propeller gene of the isolates was sequenced. A search for non-synonymous mutations in the propeller region of K13 was performed in the 181 isolates collected from Dakar from 2015 to 2016. Three synonymous mutations were detected (D464D, C469C and R471R). Of 119 patients treated with i.v. artesunate or intramuscular artemether followed by artemether/lumefantrine, 9 patients were still parasitaemic on Day 3. Parasites from these nine patients were wild-type for K13-propeller. None of the polymorphisms known to be involved in artemisinin resistance in Asia were detected. These results suggest that K13 is not the best predictive marker for artemisinin resistance in Africa. More isolates from clinical failure cases or patients with delayed parasite clearance after treatment with artemisinin derivatives are necessary to identify new molecular markers. |
[Mh] Termos MeSH primário: |
Antimaláricos/uso terapêutico Artemisininas/uso terapêutico Malária Falciparum/tratamento farmacológico Malária Falciparum/parasitologia Plasmodium falciparum/efeitos dos fármacos Polimorfismo Genético Proteínas de Protozoários/genética
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[Mh] Termos MeSH secundário: |
Animais Seres Humanos Mutação de Sentido Incorreto Plasmodium falciparum/genética Plasmodium falciparum/isolamento & purificação Mutação Puntual Senegal Análise de Sequência de DNA Fatores de Tempo Resultado do Tratamento
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[Pt] Tipo de publicação: | JOURNAL ARTICLE |
[Nm] Nome de substância:
| 0 (Antimalarials); 0 (Artemisinins); 0 (Protozoan Proteins); 9RMU91N5K2 (artemisinine) |
[Em] Mês de entrada: | 1802 |
[Cu] Atualização por classe: | 180220 |
[Lr] Data última revisão:
| 180220 |
[Sb] Subgrupo de revista: | IM |
[Da] Data de entrada para processamento: | 170429 |
[St] Status: | MEDLINE |
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