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  1 / 12621 MEDLINE  
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[PMID]:28449707
[Au] Autor:Llorens F; Thüne K; Sikorska B; Schmitz M; Tahir W; Fernández-Borges N; Cramm M; Gotzmann N; Carmona M; Streichenberger N; Michel U; Zafar S; Schuetz AL; Rajput A; Andréoletti O; Bonn S; Fischer A; Liberski PP; Torres JM; Ferrer I; Zerr I
[Ad] Endereço:Department of Neurology, University Medical Center Göttingen, and German Center for Neurodegenerative Diseases (DZNE), Robert Koch Strasse 40, 37075, Göttingen, Germany. franc.llorens@gmail.com.
[Ti] Título:Altered Ca homeostasis induces Calpain-Cathepsin axis activation in sporadic Creutzfeldt-Jakob disease.
[So] Source:Acta Neuropathol Commun;5(1):35, 2017 04 27.
[Is] ISSN:2051-5960
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Sporadic Creutzfeldt-Jakob disease (sCJD) is the most prevalent form of human prion disease and it is characterized by the presence of neuronal loss, spongiform degeneration, chronic inflammation and the accumulation of misfolded and pathogenic prion protein (PrP ). The molecular mechanisms underlying these alterations are largely unknown, but the presence of intracellular neuronal calcium (Ca ) overload, a general feature in models of prion diseases, is suggested to play a key role in prion pathogenesis.Here we describe the presence of massive regulation of Ca responsive genes in sCJD brain tissue, accompanied by two Ca -dependent processes: endoplasmic reticulum stress and the activation of the cysteine proteases Calpains 1/2. Pathogenic Calpain proteins activation in sCJD is linked to the cleavage of their cellular substrates, impaired autophagy and lysosomal damage, which is partially reversed by Calpain inhibition in a cellular prion model. Additionally, Calpain 1 treatment enhances seeding activity of PrP in a prion conversion assay. Neuronal lysosomal impairment caused by Calpain over activation leads to the release of the lysosomal protease Cathepsin S that in sCJD mainly localises in axons, although massive Cathepsin S overexpression is detected in microglial cells. Alterations in Ca homeostasis and activation of Calpain-Cathepsin axis already occur at pre-clinical stages of the disease as detected in a humanized sCJD mouse model.Altogether our work indicates that unbalanced Calpain-Cathepsin activation is a relevant contributor to the pathogenesis of sCJD at multiple molecular levels and a potential target for therapeutic intervention.
[Mh] Termos MeSH primário: Encéfalo/metabolismo
Cálcio/metabolismo
Calpaína/metabolismo
Catepsinas/metabolismo
Síndrome de Creutzfeldt-Jakob/metabolismo
Homeostase/fisiologia
[Mh] Termos MeSH secundário: Animais
Encéfalo/patologia
Cátions Bivalentes/metabolismo
Células Cultivadas
Síndrome de Creutzfeldt-Jakob/patologia
Modelos Animais de Doenças
Seres Humanos
Lisossomos/metabolismo
Lisossomos/patologia
Mesocricetus
Camundongos Transgênicos
Neurônios/metabolismo
Neurônios/patologia
Proteínas PrPSc/metabolismo
Ratos Wistar
Proteínas Recombinantes/metabolismo
Ovinos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Cations, Divalent); 0 (PrPSc Proteins); 0 (Recombinant Proteins); EC 3.4.- (Cathepsins); EC 3.4.22.- (Calpain); SY7Q814VUP (Calcium)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:180307
[Lr] Data última revisão:
180307
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170429
[St] Status:MEDLINE
[do] DOI:10.1186/s40478-017-0431-y


  2 / 12621 MEDLINE  
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[PMID]:29297910
[Au] Autor:Pedreira-Segade U; Michot LJ; Daniel I
[Ad] Endereço:Univ Lyon, Ens de Lyon, Université Lyon 1, CNRS, UMR 5276 LGL-TPE, F-69342, Lyon, France. ulysse.pedreira@ens-lyon.org.
[Ti] Título:Effects of salinity on the adsorption of nucleotides onto phyllosilicates.
[So] Source:Phys Chem Chem Phys;20(3):1938-1952, 2018 Jan 17.
[Is] ISSN:1463-9084
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:In the context of the origin of life, phyllosilicate surfaces might favor the adsorption, concentration and reactivity of otherwise diluted prebiotic molecules. The primitive oceanic seafloor was certainly rich in Fe-Mg-rich phyllosilicates. The salinity of the primitive seawater remains largely unknown. Values ranging from 1 to 15 times modern salinity have been proposed and the salt composition of the primitive ocean also remains elusive although it may have played a role in the interactions between nucleotides and mineral surfaces. Therefore we studied the adsorption of 5'-monophosphate deoxyguanosine (dGMP) as a model nucleotide onto a Fe-rich swelling clay, i.e. nontronite, and an Al-rich phyllosilicate, i.e. pyrophyllite, for comparison. Experiments were carried out at atmospheric pressure, 25 °C and natural pH, with a series of salts NaCl, MgCl , CaCl , MgSO , NaH PO and LaCl in order to evaluate the effect of cations and anions on dGMP adsorption. The present study shows that nucleotides are adsorbed on both phyllosilicates via a ligand exchange mechanism. The phosphate group of the nucleotide is adsorbed on the lateral metal hydroxyls of the broken edges of phyllosilicates. The presence of divalent cations or molecular anions, such as phosphate or sulfate, tends to inhibit this interaction on mineral surfaces. However, in the presence of divalent cations, cationic bridging on the basal surfaces of the swelling clay also occurs and could induce a higher retention capacity of the swelling clays compared to non-swelling phyllosilicates in primitive and modern natural environments.
[Mh] Termos MeSH primário: Silicatos de Alumínio/química
Nucleotídeos/química
[Mh] Termos MeSH secundário: Adsorção
Cátions Bivalentes/química
Nucleotídeos de Desoxiguanina/química
Concentração de Íons de Hidrogênio
Sais/química
Espalhamento a Baixo Ângulo
Difração de Raios X
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Aluminum Silicates); 0 (Cations, Divalent); 0 (Deoxyguanine Nucleotides); 0 (Nucleotides); 0 (Salts); 1302-87-0 (clay); 7EAM4TG712 (2'-deoxyguanosine 5'-phosphate)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180220
[Lr] Data última revisão:
180220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180104
[St] Status:MEDLINE
[do] DOI:10.1039/c7cp07004g


  3 / 12621 MEDLINE  
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[PMID]:29235789
[Au] Autor:Danylovych YV; Chunikhin AY; Danylovych GV; Kolomiets OV
[Ti] Título:The use of the Petri net method in the simulation modeling of mitochondrial swelling.
[So] Source:Ukr Biochem J;88(4):66-74, 2016 Jul-Aug.
[Is] ISSN:2409-4943
[Cp] País de publicação:Ukraine
[La] Idioma:eng
[Ab] Resumo:Using photon correlation spectroscopy, which allows investigating changes in the hydrodynamic dia­meter of the particles in suspension, it was shown that ultrahigh concentrations of Ca2+ (over 10 mM) induce swelling of isolated mitochondria. An increase in hydrodynamic diameter was caused by an increase of non-specific mitochondrial membrane permeability to Ca ions, matrix Ca2+ overload, activation of ATP- and Ca2+-sensitive K+-channels, as well as activation of cyclosporin-sensitive permeability transition pore. To formalize the experimental data and to assess conformity of experimental results with theoretical predictions we developed a simulation model using the hybrid functional Petri net method.
[Mh] Termos MeSH primário: Cálcio/farmacologia
Ciclosporina/farmacologia
Mitocôndrias/efeitos dos fármacos
Dilatação Mitocondrial/efeitos dos fármacos
Modelos Biológicos
[Mh] Termos MeSH secundário: Animais
Cálcio/metabolismo
Cátions Bivalentes
Permeabilidade da Membrana Celular/efeitos dos fármacos
Simulação por Computador
Feminino
Transporte de Íons
Canais KATP/metabolismo
Cinética
Mitocôndrias/metabolismo
Proteínas de Transporte da Membrana Mitocondrial/metabolismo
Miométrio/química
Miométrio/metabolismo
Canais de Potássio Cálcio-Ativados/metabolismo
Ratos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cations, Divalent); 0 (KATP Channels); 0 (Mitochondrial Membrane Transport Proteins); 0 (Potassium Channels, Calcium-Activated); 0 (mitochondrial permeability transition pore); 83HN0GTJ6D (Cyclosporine); SY7Q814VUP (Calcium)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180116
[Lr] Data última revisão:
180116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171214
[St] Status:MEDLINE
[do] DOI:10.15407/ubj88.04.066


  4 / 12621 MEDLINE  
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[PMID]:29235753
[Ti] Título:The influence of heavy metal ions, spermine and sodium nitroprusside on ATP-hydrolases of cell membranes of rat colon smooth muscle.
[So] Source:Ukr Biochem J;88(4):20-8, 2016 Jul-Aug.
[Is] ISSN:2409-4943
[Cp] País de publicação:Ukraine
[La] Idioma:eng
[Ab] Resumo:The specific features of functional lability of the rat colon smooth muscle (CSM) АТР-hydrolases were studied. Na+,K+-AТРase activity is effectively inhibited by divalent ions of both transition (≥ 0,1 µM) and nontransition (≥ 1 µM) heavy metals in succession by efficiency: Cu2+ > Fe2+ ≥ Cd2+ (10 µM). Polyamine spermine (0,5-1,0 mM) is a weak Na+,K+-AТРase inhibitor at saturation concentrations of ions and substrate. Sodium nitroprusside (1 mM) as nitric oxide-generating compound exhibits weak Na+,K+-AТРase inhibition only after prolonged preincubation with membranes. Mg2+-АТР-hydrolase activity in all cases is much more resistant to studied agents. Considering the example of the CSM Na+,K+-AТРase it is assumed that enzyme has specific biochemical features that contribute to its role as a potential target and redox-sensor, mediating the pathological mechanisms of heavy metal intoxication and cell oxidative damage.
[Mh] Termos MeSH primário: Adenosina Trifosfatases/metabolismo
Membrana Celular/efeitos dos fármacos
Metais Pesados/farmacologia
Nitroprussiato/farmacologia
ATPase Trocadora de Sódio-Potássio/metabolismo
Espermina/farmacologia
[Mh] Termos MeSH secundário: Adenosina Trifosfatases/antagonistas & inibidores
Animais
Cádmio/farmacologia
Cátions Bivalentes
Fracionamento Celular
Membrana Celular/metabolismo
Colo/citologia
Colo/enzimologia
Cobre/farmacologia
Ferro/farmacologia
Cinética
Masculino
Músculo Liso/citologia
Músculo Liso/enzimologia
Miócitos de Músculo Liso/citologia
Miócitos de Músculo Liso/enzimologia
Ratos
Ratos Wistar
ATPase Trocadora de Sódio-Potássio/antagonistas & inibidores
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cations, Divalent); 0 (Metals, Heavy); 00BH33GNGH (Cadmium); 169D1260KM (Nitroprusside); 2FZ7Y3VOQX (Spermine); 789U1901C5 (Copper); E1UOL152H7 (Iron); EC 3.6.1.- (Adenosine Triphosphatases); EC 3.6.1.- (magnesium sodium ATPase); EC 3.6.3.9 (Sodium-Potassium-Exchanging ATPase)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180116
[Lr] Data última revisão:
180116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171214
[St] Status:MEDLINE
[do] DOI:10.15407/ubj88.04.020


  5 / 12621 MEDLINE  
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[PMID]:28941825
[Au] Autor:Smith AT; Sestok AE
[Ad] Endereço:Department of Chemistry and Biochemistry, University of Maryland, Baltimore County, Baltimore, MD, 21250, USA. Electronic address: smitha@umbc.edu.
[Ti] Título:Expression and purification of functionally active ferrous iron transporter FeoB from Klebsiella pneumoniae.
[So] Source:Protein Expr Purif;142:1-7, 2018 Feb.
[Is] ISSN:1096-0279
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The acquisition of ferrous iron (Fe ) is an important virulence factor utilized by several hospital-acquired (nosocomial) pathogens such as Klebsiella pneumoniae to establish infection within human hosts. Virtually all bacteria use the ferrous iron transport system (Feo) to acquire ferrous iron from their environments, which are often biological niches that stabilize Fe relative to Fe . However, the details of this process remain poorly understood, likely owing to the few expression and purification systems capable of supplying sufficient quantities of the chief component of the Feo system, the integral membrane GTPase FeoB. This bottleneck has undoubtedly hampered efforts to understand this system in order to target it for therapeutic intervention. In this study, we describe the expression, solubilization, and purification of the Fe transporter from K. pneumoniae, KpFeoB. We show that this protein may be heterologously overexpressed in Escherichia coli as the host organism. After testing several different commercially-available detergents, we have developed a solubilization and purification protocol that produces milligram quantities of KpFeoB with sufficient purity for enzymatic and biophysical analyses. Importantly, we demonstrate that KpFeoB displays robust GTP hydrolysis activity (k of ∼10 s ) in the absence of any additional stimulatory factors. Our findings suggest that K. pneumoniae may be capable of using its Feo system to drive Fe import in an active manner.
[Mh] Termos MeSH primário: Proteínas de Bactérias/genética
Proteínas de Transporte de Cátions/genética
Guanosina Trifosfato/metabolismo
Ferro/metabolismo
Klebsiella pneumoniae/química
[Mh] Termos MeSH secundário: Proteínas de Bactérias/química
Proteínas de Bactérias/isolamento & purificação
Proteínas de Bactérias/metabolismo
Proteínas de Transporte de Cátions/química
Proteínas de Transporte de Cátions/isolamento & purificação
Proteínas de Transporte de Cátions/metabolismo
Cátions Bivalentes
Clonagem Molecular
Detergentes/química
Ensaios Enzimáticos
Escherichia coli/genética
Escherichia coli/metabolismo
Expressão Gênica
Hidrólise
Transporte de Íons
Cinética
Klebsiella pneumoniae/enzimologia
Maltose/análogos & derivados
Maltose/química
Plasmídeos/química
Plasmídeos/metabolismo
Polietilenoglicóis/química
Conformação Proteica em alfa-Hélice
Proteínas Recombinantes/química
Proteínas Recombinantes/genética
Proteínas Recombinantes/isolamento & purificação
Proteínas Recombinantes/metabolismo
Solubilidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Cation Transport Proteins); 0 (Cations, Divalent); 0 (Detergents); 0 (Recombinant Proteins); 0 (dodecyl maltopyranoside); 3055-98-9 (dodecyloctaethyleneglycol monoether); 30IQX730WE (Polyethylene Glycols); 69-79-4 (Maltose); 86-01-1 (Guanosine Triphosphate); E1UOL152H7 (Iron)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171128
[Lr] Data última revisão:
171128
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170925
[St] Status:MEDLINE


  6 / 12621 MEDLINE  
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[PMID]:28741460
[Au] Autor:Wei S; Zhang XY; Sun Y; Conway LP; Liu L
[Ad] Endereço:Glycomics and Glycan Bioengineering Research Center (GGBRC), College of Food Science and Technology, Nanjing Agricultural University, Nanjing. China.
[Ti] Título:Discovery and Biochemical Characterization of UDP-Glucose Dehydrogenase from Akkermansia muciniphila.
[So] Source:Protein Pept Lett;24(8):735-741, 2017.
[Is] ISSN:1875-5305
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: The biocatalytic oxidation of UDP-glucose in the presence of NAD+ is catalyzed by UDP-glucose dehydrogenases. OBJECTIVES: The main objective of this study was the characterization of a UDP-glucose dehydrogenase (AmUGD) from Akkermansia muciniphila, a bacterium originally isolated from human faeces in an anaerobic medium containing gastric mucin as the sole carbon source. METHODS: The biochemical analysis of AmUGD was performed using a plate reader-based assay measuring the reaction by-product NADH. Furthermore, HPLC- and MALDI-ToF-MS- based methods were used for the enzyme characterization. RESULTS: The recombinant form of the protein was expressed in E. coli and the purified enzyme exhibited optimum levels of activity at 37°C and pH 9.0. While the enzyme is active in the absence of metal ions, the presence of Zn2+ ions results in markedly enhanced levels of catalysis. CONCLUSION: This study describes the first characterization of a nucleotide-processing enzyme from A. muciniphila. The ease of expression and purification of this enzyme make it ideal for biotechnological applications such as the enzymatic synthesis of nucleotide sugars, which may in turn be used for the synthesis of complex carbohydrates or glycoconjugates.
[Mh] Termos MeSH primário: Proteínas de Bactérias/metabolismo
NAD/metabolismo
Uridina Difosfato Glucose Desidrogenase/metabolismo
Uridina Difosfato Glucose/metabolismo
Verrucomicrobia/química
[Mh] Termos MeSH secundário: Proteínas de Bactérias/genética
Cátions Bivalentes
Clonagem Molecular
Escherichia coli/genética
Escherichia coli/metabolismo
Expressão Gênica
Temperatura Alta
Concentração de Íons de Hidrogênio
Cinética
NAD/química
Plasmídeos/química
Plasmídeos/metabolismo
Proteínas Recombinantes/genética
Proteínas Recombinantes/metabolismo
Especificidade por Substrato
Uridina Difosfato Glucose/química
Uridina Difosfato Glucose Desidrogenase/genética
Verrucomicrobia/enzimologia
Zinco/química
Zinco/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Cations, Divalent); 0 (Recombinant Proteins); 0U46U6E8UK (NAD); EC 1.1.1.22 (Uridine Diphosphate Glucose Dehydrogenase); J41CSQ7QDS (Zinc); V50K1D7P4Y (Uridine Diphosphate Glucose)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171128
[Lr] Data última revisão:
171128
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170726
[St] Status:MEDLINE
[do] DOI:10.2174/0929866524666170724111147


  7 / 12621 MEDLINE  
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[PMID]:29065131
[Au] Autor:Fang X; Liang P; Raba DA; Rosas-Lemus M; Chakravarthy S; Tuz K; Juárez O
[Ad] Endereço:Department of Biological Sciences, Illinois Institute of Technology, Chicago, Illinois, United States of America.
[Ti] Título:Kinetic characterization of Vibrio cholerae ApbE: Substrate specificity and regulatory mechanisms.
[So] Source:PLoS One;12(10):e0186805, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:ApbE is a member of a novel family of flavin transferases that incorporates flavin mononucleotide (FMN) to subunits of diverse respiratory complexes, which fulfill important homeostatic functions. In this work a detailed characterization of Vibrio cholerae ApbE physiologic activity, substrate specificity and pH dependency was carried out. The data obtained show novel characteristics of the regulation and function of this family. For instance, our experiments indicate that divalent cations are essential for ApbE function, and that the selectivity depends largely on size and the coordination sphere of the cation. Our data also show that ApbE regulation by pH, ADP and potassium is an important mechanism that enhances the adaptation, survival and colonization of V. cholerae in the small intestine. Moreover, studies of the pH-dependency of the activity show that the reaction is favored under alkaline conditions, with a pKa of 8.4. These studies, together with sequence and structure analysis allowed us to identify His257, which is absolutely conserved in the family, as a candidate for the residue whose deprotonation controls the activity. Remarkably, the mutant H257G abolished the flavin transfer activity, strongly indicating that this residue plays an important role in the catalytic mechanism of ApbE.
[Mh] Termos MeSH primário: Proteínas de Bactérias/metabolismo
Vibrio cholerae/metabolismo
[Mh] Termos MeSH secundário: Cátions Bivalentes
Cátions Monovalentes
Concentração de Íons de Hidrogênio
Cinética
Especificidade por Substrato
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Cations, Divalent); 0 (Cations, Monovalent)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171113
[Lr] Data última revisão:
171113
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171025
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0186805


  8 / 12621 MEDLINE  
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[PMID]:29017922
[Au] Autor:Denesyuk AI; Permyakov SE; Johnson MS; Permyakov EA; Denessiouk K
[Ad] Endereço:Faculty of Science and Engineering, Åbo Akademi University, Turku 20500, Finland; Institute for Biological Instrumentation of the Russian Academy of Sciences, Pushchino 142290, Russia. Electronic address: adenesyu@abo.fi.
[Ti] Título:Building kit for metal cation binding sites in proteins.
[So] Source:Biochem Biophys Res Commun;494(1-2):311-317, 2017 Dec 09.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Starting with conformations of calcium-binding sites in parvalbumin and integrin (representative structures of EF-hand and calcium blade zones, respectively) we introduce four new different local Ca -recognition units in proteins: a one-residue unit type I (ORI); a three-residue unit type I (TRI); a one-residue unit type II (ORII) and a three-residue unit type II (TRII). Based on the amount and nature of variable atoms, the type I and II units theoretically can have four and twelve variants, respectively. Analysis of known "Ca -bound functional niches" in proteins revealed presence of almost all possible variants of Ca -recognition units in actual structures. Parvalbumin, integrin alpha-IIb and sixteen other proteins with different Ca -bound functional niches contain various consecutively joined combinations of OR(I/II) and TR(I/II) units. Such a OR(I/II)+TR(I/II) joint unit forms a tripeptide, which uses three main-chain atoms for metal binding: nitrogen (Donor), oxygen (Acceptor) and nitrogen (Donor). Thus, taken together, the described ORI, TRI, ORII and TRII units can serve as elementary blocks to construct more complex calcium recognizing substructures in a variety of calcium binding sites of unrelated proteins.
[Mh] Termos MeSH primário: Proteínas de Ligação ao Cálcio/química
Cálcio/química
Integrinas/química
Parvalbuminas/química
[Mh] Termos MeSH secundário: Animais
Cátions Bivalentes
Motivos EF Hand
Seres Humanos
Nitrogênio/química
Oxigênio/química
Ligação Proteica
Termodinâmica
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Calcium-Binding Proteins); 0 (Cations, Divalent); 0 (Integrins); 0 (Parvalbumins); N762921K75 (Nitrogen); S88TT14065 (Oxygen); SY7Q814VUP (Calcium)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171108
[Lr] Data última revisão:
171108
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171012
[St] Status:MEDLINE


  9 / 12621 MEDLINE  
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[PMID]:28937994
[Au] Autor:Hakobyan D; Gerke V; Heuer A
[Ad] Endereço:Institute of Physical Chemistry, University of Muenster, Muenster, Germany.
[Ti] Título:Modeling of annexin A2-Membrane interactions by molecular dynamics simulations.
[So] Source:PLoS One;12(9):e0185440, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The annexins are a family of Ca2+-regulated phospholipid binding proteins that are involved in membrane domain organization and membrane trafficking. Although they are widely studied and crystal structures are available for several soluble annexins their mode of membrane association has never been studied at the molecular level. Here we obtained molecular information on the annexin-membrane interaction that could serve as paradigm for the peripheral membrane association of cytosolic proteins by Molecular Dynamics simulations. We analyzed systems containing the monomeric annexin A2 (AnxA2), a membrane with negatively charged phosphatidylserine (POPS) lipids as well as Ca2+ ions. On the atomic level we identify the AnxA2 orientations and the respective residues which display the strongest interaction with Ca2+ ions and the membrane. The simulation results fully agree with earlier experimental findings concerning the positioning of bound Ca2+ ions. Furthermore, we identify for the first time a significant interaction between lysine residues of the protein and POPS lipids that occurs independently of Ca2+ suggesting that AnxA2-membrane interactions can also occur in a low Ca2+ environment. Finally, by varying Ca2+ concentrations and lipid composition in our simulations we observe a calcium-induced negative curvature of the membrane as well as an AnxA2-induced lipid ordering.
[Mh] Termos MeSH primário: Anexina A2/metabolismo
Membranas Artificiais
Simulação de Dinâmica Molecular
[Mh] Termos MeSH secundário: Cálcio/metabolismo
Cátions Bivalentes/metabolismo
Citosol/metabolismo
Dimerização
Fosfatidilserinas/química
Ligação Proteica
Propriedades de Superfície
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Annexin A2); 0 (Cations, Divalent); 0 (Membranes, Artificial); 0 (Phosphatidylserines); 40290-44-6 (1-palmitoyl-2-oleoylglycero-3-phosphoserine); SY7Q814VUP (Calcium)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171017
[Lr] Data última revisão:
171017
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170923
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0185440


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[PMID]:28934354
[Au] Autor:Serrano-Aroca Á; Deb S
[Ad] Endereço:Bioengineering & Cellular Therapy Group, Facultad de Veterinaria y Ciencias Aplicadas, Universidad Católica de Valencia "San Vicente Mártir", C/Guillem de Castro 94,Valencia, Spain.
[Ti] Título:Synthesis of irregular graphene oxide tubes using green chemistry and their potential use as reinforcement materials for biomedical applications.
[So] Source:PLoS One;12(9):e0185235, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Micrometer length tubes of graphene oxide (GO) with irregular form were synthesised following facile and green metal complexation reactions. These materials were obtained by crosslinking of GO with calcium, zinc or strontium chlorides at three different temperatures (24, 34 and 55°C) using distilled water as solvent for the compounds and following a remarkably simple and low-cost synthetic method, which employs no hazardous substances and is conducted without consumption of thermal or sonic energy. These irregular continuous GO networks showed a very particular interconnected structure by Field Emission Scanning Electron Microscopy with Energy-Disperse X-Ray Spectroscopy for elemental analysis and High-resolution Transmission Electron Microscopy with Scanning Transmission Electron Microscope Dark Field Imaging, and were analysed by Raman Spectroscopy. To demonstrate the potential use of these 3D GO networks as reinforcement materials for biomedical applications, two composites of calcium alginate with irregular tubes of GO and with single GO nanosheets were prepared with the same amount of GO and divalent atoms and analysed. Thus, the dynamic-mechanical modulus of the composites synthesised with the 3D crosslinked GO networks showed a very significant mechanical improvement due to marked microstructural changes confirmed by confocal microscopy, differential scanning calorimetry and Fourier transform infrared spectroscopy.
[Mh] Termos MeSH primário: Materiais Biocompatíveis/química
Grafite/química
Fenômenos Mecânicos
Óxidos/química
[Mh] Termos MeSH secundário: Cátions Bivalentes/química
Técnicas de Química Sintética
Química Verde
Temperatura Ambiente
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biocompatible Materials); 0 (Cations, Divalent); 0 (Oxides); 7782-42-5 (Graphite)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171018
[Lr] Data última revisão:
171018
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170922
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0185235



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