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Pesquisa : D01.268.150.200.550 [Categoria DeCS]
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  1 / 271 MEDLINE  
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[PMID]:29324795
[Au] Autor:Iyer JK; Dickey A; Rouhani P; Kaul A; Govindaraju N; Singh RN; Kaul R
[Ad] Endereço:Department of Biochemistry and Microbiology, Oklahoma State University-Center for Health Sciences, Tulsa, Oklahoma, United States of America.
[Ti] Título:Nanodiamonds facilitate killing of intracellular uropathogenic E. coli in an in vitro model of urinary tract infection pathogenesis.
[So] Source:PLoS One;13(1):e0191020, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:About 25-44% of women will experience at least one episode of recurrent UTI and the causative agent in over 70% of UTI cases is uropathogenic Escherichia coli (UPEC). UPEC cause recurrent UTI by evading the bladder's innate immune system through internalization into the bladder epithelium where antibiotics cannot reach or be effective. Thus, it is important to develop novel therapeutics to eliminate these intracellular pathogens. Nanodiamonds (NDs) are biocompatible nanomaterials that serve as promising candidates for targeted therapeutic applications. The objective of the current study was to investigate if 6 or 25 nm NDs can kill extracellular and intracellular UPEC in infected bladder cells. We utilized the human bladder epithelial cell line, T24, and an invasive strain of UPEC that causes recurrent UTI. We found that acid-purified 6 nm NDs displayed greater antibacterial properties towards UPEC than 25 nm NDs (11.5% vs 94.2% CFU/mL at 100 µg/mL of 6 and 25 nm, respectively; P<0.001). Furthermore, 6 nm NDs were better than 25 nm NDs in reducing the number of UPEC internalized in T24 bladder cells (46.1% vs 81.1% CFU/mL at 100 µg/mL of 6 and 25 nm, respectively; P<0.01). Our studies demonstrate that 6 nm NDs interacted with T24 bladder cells in a dose-dependent manner and were internalized in 2 hours through an actin-dependent mechanism. Finally, internalization of NDs was required for reducing the number of intracellular UPEC in T24 bladder cells. These findings suggest that 6 nm NDs are promising candidates to treat recurrent UTIs.
[Mh] Termos MeSH primário: Nanodiamantes
Infecções Urinárias/microbiologia
Escherichia coli Uropatogênica/efeitos dos fármacos
[Mh] Termos MeSH secundário: Linhagem Celular
Contagem de Colônia Microbiana
Seres Humanos
Técnicas In Vitro
Microscopia Confocal
Microscopia Eletrônica de Transmissão
Análise Espectral Raman
Bexiga Urinária/citologia
Bexiga Urinária/microbiologia
Bexiga Urinária/ultraestrutura
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Nanodiamonds)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180112
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0191020


  2 / 271 MEDLINE  
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[PMID]:29200855
[Au] Autor:Gerstenhaber JA; Barone FC; Marcinkiewicz C; Li J; Shiloh AO; Sternberg M; Lelkes PI; Feuerstein G
[Ad] Endereço:Department of Bioengineering, College of Engineering, Temple University, Philadelphia, PA.
[Ti] Título:Vascular thrombus imaging in vivo via near-infrared fluorescent nanodiamond particles bioengineered with the disintegrin bitistatin (Part II).
[So] Source:Int J Nanomedicine;12:8471-8482, 2017.
[Is] ISSN:1178-2013
[Cp] País de publicação:New Zealand
[La] Idioma:eng
[Ab] Resumo:The aim of this feasibility study was to test the ability of fluorescent nanodiamond particles (F-NDP) covalently conjugated with bitistatin (F-NDP-Bit) to detect vascular blood clots in vivo using extracorporeal near-infrared (NIR) imaging. Specifically, we compared NIR fluorescence properties of F-NDP with N-V (F-NDP ) and N-V-N color centers and sizes (100-10,000 nm). Optimal NIR fluorescence and tissue penetration across biological tissues (rat skin, porcine axillary veins, and skin) was obtained for F-NDP with a mean diameter of 700 nm. Intravital imaging (using in vivo imaging system [IVIS]) in vitro revealed that F-NDP -loaded glass capillaries could be detected across 6 mm of rat red-muscle barrier and 12 mm of porcine skin, which equals the average vertical distance of a human carotid artery bifurcation from the surface of the adjacent skin (14 mm). In vivo, feasibility was demonstrated in a rat model of occlusive blood clots generated using FeCl in the carotid artery bifurcation. Following systemic infusions of F-NDP -Bit (3 or 15 mg/kg) via the external carotid artery or femoral vein (N=3), presence of the particles in the thrombi was confirmed both in situ via IVIS, and ex vivo via confocal imaging. The presence of F-NDP in the vascular clots was further confirmed by direct counting of fluorescent particles extracted from clots following tissue solubilization. Our data suggest that F-NDP -Bit associate with vascular blood clots, presumably by binding of F-NDP -Bit to activated platelets within the blood clot. We posit that F-NDP -Bit could serve as a noninvasive platform for identification of vascular thrombi using NIR energy monitored by an extracorporeal device.
[Mh] Termos MeSH primário: Bioengenharia/métodos
Diagnóstico por Imagem
Desintegrinas/química
Raios Infravermelhos
Nanodiamantes/química
Peptídeos/química
Trombose/diagnóstico
[Mh] Termos MeSH secundário: Animais
Artérias Carótidas/patologia
Modelos Animais de Doenças
Desintegrinas/administração & dosagem
Fluorescência
Seres Humanos
Infusões Intravenosas
Masculino
Peptídeos/administração & dosagem
Ratos Sprague-Dawley
Suínos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Disintegrins); 0 (Nanodiamonds); 0 (Peptides); 124123-27-9 (bitistatin)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171205
[St] Status:MEDLINE
[do] DOI:10.2147/IJN.S146946


  3 / 271 MEDLINE  
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[PMID]:28982373
[Au] Autor:Pham NB; Ho TT; Nguyen GT; Le TT; Le NT; Chang HC; Pham MD; Conrad U; Chu HH
[Ad] Endereço:Institute of Biotechnology, Vietnam Academy of Science and Technology, Ha Noi, Vietnam.
[Ti] Título:Nanodiamond enhances immune responses in mice against recombinant HA/H7N9 protein.
[So] Source:J Nanobiotechnology;15(1):69, 2017 Oct 05.
[Is] ISSN:1477-3155
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: The continuing spread of the newly emerged H7N9 virus among poultry in China, as well as the possibility of human-to-human transmission, has attracted numerous efforts to develop an effective vaccine against H7N9. The use of nanoparticles in vaccinology is inspired by the fact that most pathogens have a dimension within the nano-size range and therefore can be processed efficiently by the immune system, which leads to a potent immune response. Herein, we report a facile approach to increase antigen size to achieve not only fast but also effective responses against the recombinant HA/H7N9 protein via a simple conjugation of the protein onto the surface of nanodiamond particles. RESULTS: In this study, trimeric Haemagglutinin (H7) that is transiently expressed in N. benthamiana was purified using affinity chromatography, and its trimeric state was revealed successfully by the cross-linking reaction. The trimeric H7 solution was subsequently mixed with a nanodiamond suspension in different ratios. The successful conjugation of the trimeric H7 onto the surface of nanodiamond particles was demonstrated by the changes in size and Zeta-potential of the particles before and after protein coating, Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), and Western-blot analysis. Next, biofunction of the protein-nanodiamond conjugates was screened using a haemagglutination assay. A mixture containing 5 µg of trimeric H7 and 60 µg of nanodiamond corresponds to a ratio of 1:12 (w/w) of agglutinated chicken red blood cells at HA titer of 1024, which is 512-fold higher than the HA titer of free trimeric H7. After the 2nd and 3rd immunization in mice, ELISA and Western blot analyses demonstrated that the physical mixture of trimeric H7 protein and nanodiamond (1:12, w/w) elicited statistically significant stronger H7-specific-IgG response demonstrated by higher amounts of H7N9-specific IgG (over 15.4-fold with P < 0.05 after the second immunization). CONCLUSIONS: These results indicated a potential effect inherent to nanodiamond towards modulating immune systems, which should be further evaluated and broadly applied in nanovaccine development.
[Mh] Termos MeSH primário: Glicoproteínas de Hemaglutininação de Vírus da Influenza/imunologia
Vírus da Influenza A Subtipo H7N9/imunologia
Vacinas contra Influenza/imunologia
Nanodiamantes
Infecções por Orthomyxoviridae/prevenção & controle
[Mh] Termos MeSH secundário: Animais
Formação de Anticorpos
Feminino
Glicoproteínas de Hemaglutininação de Vírus da Influenza/química
Glicoproteínas de Hemaglutininação de Vírus da Influenza/uso terapêutico
Seres Humanos
Imunoglobulina G/imunologia
Vacinas contra Influenza/química
Vacinas contra Influenza/uso terapêutico
Influenza Humana/imunologia
Influenza Humana/prevenção & controle
Camundongos
Camundongos Endogâmicos BALB C
Nanodiamantes/química
Nanodiamantes/uso terapêutico
Nanodiamantes/ultraestrutura
Infecções por Orthomyxoviridae/imunologia
Proteínas Recombinantes/química
Proteínas Recombinantes/imunologia
Proteínas Recombinantes/uso terapêutico
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Hemagglutinin Glycoproteins, Influenza Virus); 0 (Immunoglobulin G); 0 (Influenza Vaccines); 0 (Nanodiamonds); 0 (Recombinant Proteins)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171108
[Lr] Data última revisão:
171108
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171007
[St] Status:MEDLINE
[do] DOI:10.1186/s12951-017-0305-2


  4 / 271 MEDLINE  
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[PMID]:28665485
[Au] Autor:Lin Y; Wu KT; Yu L; Heumann S; Su DS
[Ad] Endereço:Institute of Metal Research, Chinese Academy of Sciences, 72 Wenhua Road, Shenyang, 110016, P.R. China.
[Ti] Título:Efficient and Highly Selective Solvent-Free Oxidation of Primary Alcohols to Aldehydes Using Bucky Nanodiamond.
[So] Source:ChemSusChem;10(17):3497-3505, 2017 Sep 11.
[Is] ISSN:1864-564X
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Selective oxidation of alcohols to aldehydes is widely applicable to the synthesis of various green chemicals. The poor chemoselectivity for complicated primary aldehydes over state-of-the-art metal-free or metal-based catalysts represents a major obstacle for industrial application. Bucky nanodiamond is a potential green catalyst that exhibits excellent chemoselectivity and cycling stability for the selective oxidation of primary alcohols in diverse structures (22 examples, including aromatic, substituted aromatic, unsaturated, heterocyclic, and linear chain alcohols) to their corresponding aldehydes. The results are comparable to reported transition-metal catalysts including conventional Pt/C and Ru/C catalysts for certain substrates under solvent-free conditions. The possible activation process of the oxidant and substrates by the surface oxygen groups and defect species are revealed with model catalysts, ex situ electrochemical measurements, and ex situ attenuated total reflectance. The zigzag edges of sp carbon planes are shown to play a key role in these reactions.
[Mh] Termos MeSH primário: Álcoois/química
Aldeídos/química
Nanodiamantes/química
Solventes/química
[Mh] Termos MeSH secundário: Catálise
Oxidantes/química
Oxirredução
Peróxidos/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Alcohols); 0 (Aldehydes); 0 (Nanodiamonds); 0 (Oxidants); 0 (Peroxides); 0 (Solvents); 0 (tert-butyl peroxide)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170918
[Lr] Data última revisão:
170918
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170701
[St] Status:MEDLINE
[do] DOI:10.1002/cssc.201700968


  5 / 271 MEDLINE  
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[PMID]:28636640
[Au] Autor:Lake MP; Bouchard LS
[Ad] Endereço:Department of Chemistry and Biochemistry, University of California, Los Angeles, California, United States of America.
[Ti] Título:Targeted nanodiamonds for identification of subcellular protein assemblies in mammalian cells.
[So] Source:PLoS One;12(6):e0179295, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Transmission electron microscopy (TEM) can be used to successfully determine the structures of proteins. However, such studies are typically done ex situ after extraction of the protein from the cellular environment. Here we describe an application for nanodiamonds as targeted intensity contrast labels in biological TEM, using the nuclear pore complex (NPC) as a model macroassembly. We demonstrate that delivery of antibody-conjugated nanodiamonds to live mammalian cells using maltotriose-conjugated polypropylenimine dendrimers results in efficient localization of nanodiamonds to the intended cellular target. We further identify signatures of nanodiamonds under TEM that allow for unambiguous identification of individual nanodiamonds from a resin-embedded, OsO4-stained environment. This is the first demonstration of nanodiamonds as labels for nanoscale TEM-based identification of subcellular protein assemblies. These results, combined with the unique fluorescence properties and biocompatibility of nanodiamonds, represent an important step toward the use of nanodiamonds as markers for correlated optical/electron bioimaging.
[Mh] Termos MeSH primário: Imagem Molecular/métodos
Proteínas/metabolismo
[Mh] Termos MeSH secundário: Fluorescência
Células HeLa
Seres Humanos
Microscopia Eletrônica de Transmissão
Nanodiamantes/química
Nanodiamantes/ultraestrutura
Frações Subcelulares
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Nanodiamonds); 0 (Proteins)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171006
[Lr] Data última revisão:
171006
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170622
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0179295


  6 / 271 MEDLINE  
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[PMID]:28553109
[Au] Autor:Marcinkiewicz C; Gerstenhaber JA; Sternberg M; Lelkes PI; Feuerstein G
[Ad] Endereço:Department of Bioengineering, College of Engineering, Temple University, Philadelphia.
[Ti] Título:Bitistatin-functionalized fluorescent nanodiamond particles specifically bind to purified human platelet integrin receptor α ß and activated platelets.
[So] Source:Int J Nanomedicine;12:3711-3720, 2017.
[Is] ISSN:1178-2013
[Cp] País de publicação:New Zealand
[La] Idioma:eng
[Ab] Resumo:Thromboembolic events (TEE) underwrite key causes of death in developed countries. While advanced imaging technologies such as computed tomography scans serve to diagnose blood clots during acute cardiovascular events, no such technology is available in routine primary care for TEE risk assessment. Here, we describe an imaging platform technology based on bioengineered fluorescent nanodiamond particles (F-NDPs) functionalized with bitistatin (Bit), a disintegrin that specifically binds to the α ß integrin, platelet fibrinogen receptor (PFR) on activated platelets. Covalent linkage of purified Bit to F-NDP was concentration-dependent and saturable, as validated by enzyme-linked immunosorbent assay using specific anti-Bit antibodies. F-NDP-Bit interacted with purified PFR, either in immobilized or soluble form. Lotrafiban, a nonpeptide, α ß receptor antagonist, specifically blocked F-NDP-Bit-PFR complex formation. Moreover, F-NDP-Bit specifically binds to activated platelets incorporated into a clot generated by thrombin-activated rat platelet-rich plasma (PRP). Our results suggest that engineered F-NDP-Bit particles could serve as noninvasive, "real-time" optical diagnostics for clots present in blood vessels.
[Mh] Termos MeSH primário: Nanodiamantes/química
Peptídeos/química
Ativação Plaquetária/efeitos dos fármacos
Complexo Glicoproteico GPIIb-IIIa de Plaquetas/metabolismo
Receptores de Fibrinogênio/metabolismo
[Mh] Termos MeSH secundário: Animais
Benzodiazepinas/farmacologia
Plaquetas/efeitos dos fármacos
Fibrinogênio/metabolismo
Seres Humanos
Peptídeos/farmacologia
Piperidinas/farmacologia
Ratos Endogâmicos F344
Receptores de Fibrinogênio/química
Trombina/metabolismo
Trombose/diagnóstico por imagem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Nanodiamonds); 0 (Peptides); 0 (Piperidines); 0 (Platelet Glycoprotein GPIIb-IIIa Complex); 0 (Receptors, Fibrinogen); 124123-27-9 (bitistatin); 12794-10-4 (Benzodiazepines); 9001-32-5 (Fibrinogen); EC 3.4.21.5 (Thrombin); KLQ306I83X (lotrafiban)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170911
[Lr] Data última revisão:
170911
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170530
[St] Status:MEDLINE
[do] DOI:10.2147/IJN.S134128


  7 / 271 MEDLINE  
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[PMID]:28009158
[Au] Autor:Rej E; Gaebel T; Waddington DE; Reilly DJ
[Ad] Endereço:ARC Centre of Excellence for Engineered Quantum Systems, School of Physics, University of Sydney , Sydney, New South Wales 2006, Australia.
[Ti] Título:Hyperpolarized Nanodiamond Surfaces.
[So] Source:J Am Chem Soc;139(1):193-199, 2017 01 11.
[Is] ISSN:1520-5126
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The widespread use of nanodiamond as a biomedical platform for drug-delivery, imaging, and subcellular tracking applications stems from its nontoxicity and unique quantum mechanical properties. Here, we extend this functionality to the domain of magnetic resonance, by demonstrating that the intrinsic electron spins on the nanodiamond surface can be used to hyperpolarize adsorbed liquid compounds at low fields and room temperature. By combining relaxation measurements with hyperpolarization, spins on the surface of the nanodiamond can be distinguished from those in the bulk liquid. These results are likely of use in signaling the controlled release of pharmaceutical payloads.
[Mh] Termos MeSH primário: Nanodiamantes/química
[Mh] Termos MeSH secundário: Adsorção
Tamanho da Partícula
Propriedades de Superfície
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Nanodiamonds)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170825
[Lr] Data última revisão:
170825
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161224
[St] Status:MEDLINE
[do] DOI:10.1021/jacs.6b09293


  8 / 271 MEDLINE  
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[PMID]:28003120
[Au] Autor:Hou W; Toh TB; Abdullah LN; Yvonne TWZ; Lee KJ; Guenther I; Chow EK
[Ad] Endereço:Cancer Science Institute of Singapore, Yong Loo Lin School of Medicine, National University of Singapore, Singapore.
[Ti] Título:Nanodiamond-Manganese dual mode MRI contrast agents for enhanced liver tumor detection.
[So] Source:Nanomedicine;13(3):783-793, 2017 Apr.
[Is] ISSN:1549-9642
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Contrast agent-enhanced magnetic resonance (MR) imaging is critical for the diagnosis and monitoring of a number of diseases, including cancer. Certain clinical applications, including the detection of liver tumors, rely on both T1 and T2-weighted images even though contrast agent-enhanced MR imaging is not always reliable. Thus, there is a need for improved dual mode contrast agents with enhanced sensitivity. We report the development of a nanodiamond-manganese dual mode contrast agent that enhanced both T1 and T2-weighted MR imaging. Conjugation of manganese to nanodiamonds resulted in improved longitudinal and transverse relaxivity efficacy over unmodified MnCl as well as clinical contrast agents. Following intravenous administration, nanodiamond-manganese complexes outperformed current clinical contrast agents in an orthotopic liver cancer mouse model while also reducing blood serum concentration of toxic free Mn ions. Thus, nanodiamond-manganese complexes may serve as more effective dual mode MRI contrast agent, particularly in cancer.
[Mh] Termos MeSH primário: Meios de Contraste/análise
Neoplasias Hepáticas/diagnóstico por imagem
Fígado/diagnóstico por imagem
Imagem por Ressonância Magnética/métodos
Manganês/análise
Nanodiamantes/análise
[Mh] Termos MeSH secundário: Animais
Linhagem Celular
Meios de Contraste/administração & dosagem
Meios de Contraste/farmacocinética
Feminino
Seres Humanos
Manganês/administração & dosagem
Manganês/farmacocinética
Camundongos
Nanodiamantes/administração & dosagem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Contrast Media); 0 (Nanodiamonds); 42Z2K6ZL8P (Manganese)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170502
[Lr] Data última revisão:
170502
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161223
[St] Status:MEDLINE


  9 / 271 MEDLINE  
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[PMID]:27993723
[Au] Autor:Suarez-Kelly LP; Campbell AR; Rampersaud IV; Bumb A; Wang MS; Butchar JP; Tridandapani S; Yu L; Rampersaud AA; Carson WE
[Ad] Endereço:The Arthur G. James Comprehensive Cancer Center and Solove Research Institute, The Ohio State University, Columbus, OH, USA. Electronic address: lorena.suarez-kelly@osumc.edu.
[Ti] Título:Fluorescent nanodiamonds engage innate immune effector cells: A potential vehicle for targeted anti-tumor immunotherapy.
[So] Source:Nanomedicine;13(3):909-920, 2017 Apr.
[Is] ISSN:1549-9642
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Fluorescent nanodiamonds (FNDs) are nontoxic, infinitely photostable, and emit fluorescence in the near infrared region. Natural killer (NK) cells and monocytes are part of the innate immune system and are crucial to the control of carcinogenesis. FND-mediated stimulation of these cells may serve as a strategy to enhance anti-tumor activity. FNDs were fabricated with a diameter of 70±28 nm. Innate immune cell FND uptake, viability, surface marker expression, and cytokine production were evaluated in vitro. Evaluation of fluorescence emission from the FNDs was conducted in an animal model. In vitro results demonstrated that treatment of immune cells with FNDs resulted in significant dose-dependent FND uptake, no compromise in cell viability, and immune cell activation. FNDs were visualized in an animal model. Hence, FNDs may serve as novel agents with "track and trace" capabilities to stimulate innate immune cell anti-tumor responses, especially as FNDs are amenable to surface-conjugation with immunomodulatory molecules.
[Mh] Termos MeSH primário: Adjuvantes Imunológicos/uso terapêutico
Corantes Fluorescentes/uso terapêutico
Imunidade Celular/efeitos dos fármacos
Nanodiamantes/uso terapêutico
[Mh] Termos MeSH secundário: Adjuvantes Imunológicos/farmacocinética
Animais
Células Cultivadas
Corantes Fluorescentes/farmacocinética
Seres Humanos
Imunidade Inata/efeitos dos fármacos
Imunoterapia
Células Matadoras Naturais/efeitos dos fármacos
Células Matadoras Naturais/imunologia
Camundongos
Camundongos Endogâmicos BALB C
Monócitos/efeitos dos fármacos
Monócitos/imunologia
Nanodiamantes/análise
Neoplasias/imunologia
Neoplasias/terapia
Células RAW 264.7
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Adjuvants, Immunologic); 0 (Fluorescent Dyes); 0 (Nanodiamonds)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170502
[Lr] Data última revisão:
170502
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161221
[St] Status:MEDLINE


  10 / 271 MEDLINE  
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[PMID]:27935192
[Au] Autor:Broz A; Ukraintsev E; Kromka A; Rezek B; Hubalek Kalbacova M
[Ad] Endereço:Institute of Inherited Metabolic Disorders, Laboratory of Interaction of Cells with Nanomaterials, 1st Faculty of Medicine, Charles University in Prague, Ke Karlovu 2, 12853 Prague 2, Czech Republic.
[Ti] Título:Osteoblast adhesion, migration, and proliferation variations on chemically patterned nanocrystalline diamond films evaluated by live-cell imaging.
[So] Source:J Biomed Mater Res A;105(5):1469-1478, 2017 May.
[Is] ISSN:1552-4965
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Cell fate modulation by adapting the surface of a biocompatible material is nowadays a challenge in implantology, tissue engineering as well as in construction of biosensors. Nanocrystalline diamond (NCD) thin films are considered promising in these fields due to their extraordinary physical and chemical properties and diverse ways in which they can be modified structurally and chemically. The initial cell distribution, the rate of cell adhesion, distance of cell migration and also the cell proliferation are influenced by the NCD surface termination. Here, we use real-time live-cell imaging to investigate the above-mentioned processes on oxidized NCD (NCD-O) and hydrogenated NCD (NCD-H) to elucidate cell preference to the NCD-O especially on surfaces with microscopic surface termination patterns. Cells adhere more slowly and migrate farther on NCD-H than on NCD-O. Cells seeded with a fetal bovine serum (FBS) supplement in the medium move across the surface prior to adhesion. In the absence of FBS, the cells adhere immediately, but still exhibit different migration and proliferation on NCD-O/H regions. We discuss the impact of these effects on the formation of cell arrays on micropatterned NCD. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 1469-1478, 2017.
[Mh] Termos MeSH primário: Movimento Celular
Proliferação Celular
Membranas Artificiais
Nanodiamantes/química
Osteoblastos
[Mh] Termos MeSH secundário: Adesão Celular
Linhagem Celular
Seres Humanos
Osteoblastos/citologia
Osteoblastos/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Membranes, Artificial); 0 (Nanodiamonds)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171102
[Lr] Data última revisão:
171102
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161210
[St] Status:MEDLINE
[do] DOI:10.1002/jbm.a.35969



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