| [PMID]: | 28982368 |
| [Au] Autor: | Liu YZ; Chen MS; Cheng CC; Chen SH; Chen JK |
| [Ad] Endereço: | Department of Materials Science and Engineering, National Taiwan University of Science and Technology, 43, Sec 4, Keelung Rd, 106, Taipei, Taiwan, ROC. |
| [Ti] Título: | Fabrication of device with poly(N-isopropylacrylamide)-b-ssDNA copolymer brush for resistivity study. |
| [So] Source: | J Nanobiotechnology;15(1):68, 2017 Oct 05. |
| [Is] ISSN: | 1477-3155 |
| [Cp] País de publicação: | England |
| [La] Idioma: | eng |
| [Ab] Resumo: | In this study, we grafted bromo-terminated poly(N-isopropylacrylamide) (PNIPAAm) brushes onto thin gold films deposited on silicon, and then reacted with NaN to produce azido-terminated PNIPAAm brushes. A probe sequence of single-stranded DNA (ssDNA) with a 4-pentynoic acid succinimidyl ester unit was grafted onto the azido-terminated PNIPAAm brushes through a click reaction, resulting in the formation of block copolymer brushes. The PNIPAAm-b-ssDNA copolymer brushes formed supramolecular complexes stabilized by bio-multiple hydrogen bonds (BMHBs), which enhanced the proton transfer and thereby decreased the resistivity of the structures. In addition, the optimal operation window for DNA detection ranges from 0 to 0.2 M of NaCl concentration. Therefore, the specimens were prepared in the PBS solution at 150 mM NaCl concentration for target hybridization. The supramolecular complex state of the PNIPAAm-b-ssDNA copolymer brushes transformed into the phase-separated state after the hybridization with 0.5 ng/µL of its target DNA sequence owing to the competition between BMHBs and complementary hydrogen bonds. This phase transformation of the PNIPAAm and probe segments inhibited the proton transfer and significantly increased the resistivity at 25 °C. Moreover, there were no significant changes in the resistivity of the copolymer brushes after hybridization with the target sequence at 45 °C. These results indicated that the phase-separated state of the PNIPAAm-b-ssDNA copolymer brushes, which was generally occurred above the LCST, can be substantially generated after hybridization with its target DNA sequence. By performing the controlled experiments, in the same manner, using another sequence with lengths similar to that of the target sequence without complementarity. In addition, the sequences featuring various degrees of complementarity were exploited to verify the phase separation behavior inside the PNIPAAm-b-ssDNA copolymer thin film. |
| [Mh] Termos MeSH primário: |
Resinas Acrílicas/química
DNA de Cadeia Simples/química
Ouro/química
Nanoestruturas/química
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| [Mh] Termos MeSH secundário: |
DNA/análise
Impedância Elétrica
Ácidos Graxos Insaturados/química
Hibridização de Ácido Nucleico
Silício/química
|
| [Pt] Tipo de publicação: | JOURNAL ARTICLE |
[Nm] Nome de substância:
| 0 (Acrylic Resins); 0 (DNA, Single-Stranded); 0 (Fatty Acids, Unsaturated); 25189-55-3 (poly-N-isopropylacrylamide); 6089-09-4 (4-pentynoic acid); 7440-57-5 (Gold); 9007-49-2 (DNA); Z4152N8IUI (Silicon) |
| [Em] Mês de entrada: | 1711 |
| [Cu] Atualização por classe: | 171108 |
[Lr] Data última revisão:
| 171108 |
| [Sb] Subgrupo de revista: | IM |
| [Da] Data de entrada para processamento: | 171007 |
| [St] Status: | MEDLINE |
| [do] DOI: | 10.1186/s12951-017-0303-4 |
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