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[PMID]:29396269
[Au] Autor:Sezer N; Kocaoglan HO; Kiliç Ö; Lacoue-Labarthe T; Belivermis M
[Ad] Endereço:Department of Biology, Faculty of Science, Istanbul University, 34134 Vezneciler, Istanbul, Turkey.
[Ti] Título:Acidified seawater increases accumulation of cobalt but not cesium in manila clam Ruditapes philippinarum.
[So] Source:J Environ Radioact;184-185:114-121, 2018 Apr.
[Is] ISSN:1879-1700
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The pH of seawater around the world is expected to continue its decline in the near future in response to ocean acidification that is driven by heightened atmospheric CO emissions. Concomitantly, economically-important molluscs that live in coastal waters including estuaries and embayments, may be exposed to a wide assortment of contaminants, including trace metals and radionuclides. Seawater acidification may alter both the chemical speciation of select elements as well as the physiology of organisms, and may thus pose at risk to many shellfish species, including the manila clam Ruditapes philippinarum. The bioconcentration efficiency of two common radionuclides associated with the nuclear fuel cycle, Cs and Co, were investigated by exposing live clams to dissolved Cs and Co at control (pH = 8.1) and two lowered pH (pH = 7.8 and 7.5) levels using controlled aquaria. The uptake and depuration kinetics of the two radionuclides in the whole-body clam were followed for 21 and 35 days, respectively. At steady-state equilibrium, the concentration factor (CF ) for Co increased as the pH decreased (i.e. 130 ± 5, 194 ± 6, and 258 ± 10 at pH levels 8.1, 7.8 and 7.5, respectively), whereas the Cs uptake was not influenced by a change in pH conditions. During depuration, the lowest depuration rate constant of Co by the manila clam was observed at the intermediate pH of 7.8. An increase in the accumulation of Co at the intermediate pH value was thought to be caused mainly by the aragonitic shell of the clam, as well as the low salinity and alkalinity of seawater used in the experiment. Considering that accumulation consists of uptake and depuration, among the three pH conditions moderately acidified seawater enhanced most the accumulation of Co. Accumulation of Cs was not strongly influenced by a reduced pH condition, as represented by an analogous uptake constant rate and CF in each treatment. Such results suggest that future seawater pH values that are projected to be lower in the next decades, may pose a risk for calcium-bearing organisms such as shellfish.
[Mh] Termos MeSH primário: Bivalves/metabolismo
Cobalto/análise
Água do Mar/química
Poluentes Químicos da Água/análise
[Mh] Termos MeSH secundário: Animais
Césio/metabolismo
Cobalto/metabolismo
Concentração de Íons de Hidrogênio
Salinidade
Alimentos Marinhos
Poluentes Químicos da Água/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Water Pollutants, Chemical); 1KSV9V4Y4I (Cesium); 3G0H8C9362 (Cobalt)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180220
[Lr] Data última revisão:
180220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180204
[St] Status:MEDLINE


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[PMID]:29024882
[Au] Autor:Ko JA; Furuta N; Lim HB
[Ad] Endereço:Department of Chemistry, Dankook University, Yongin-si, Gyeonggi-do 448-701, South Korea; Future Environmental Research Center, Korea Institute of Toxicology, Jinju 660-844, South Korea.
[Ti] Título:Quantitative mapping of elements in basil leaves (Ocimum basilicum) based on cesium concentration and growth period using laser ablation ICP-MS.
[So] Source:Chemosphere;190:368-374, 2018 Jan.
[Is] ISSN:1879-1298
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Quantitative elemental mapping of metallic pollutants in sweet basil was studied by laser ablation (LA)-ICP-MS. For this, the sweet basil was cultivated in Hoagland nutrient solution spiked with 100 and 1000 ng mL of Cs for 10-60 days. Then, the Cs distribution in collected leaves was determined by LA-ICP-MS using lab-synthesized standard pellets based on NIST 1573a tomato leaves. For comparison, S, Ca, and K were also simultaneously determined in this measurement with a C signal from the leaves as an internal standard. The obtained calibration curves showed linear coefficient of determination (R ) of 0.991 for K and 0.999 for Cs. The concentration of Cs measured in the basil leaves increased with growth period and pollutant concentration, and accumulation followed the order of leaf margin, petiole, midrib, and veins. Although no visible symptom was detected, significant suppression of the growth rate was observed due to the presence of high-concentration Cs. The experimental model demonstrated herein showed potential for studying the influence of radioactive pollutants on plants and other organisms in the food chain.
[Mh] Termos MeSH primário: Ocimum basilicum/química
Folhas de Planta/química
[Mh] Termos MeSH secundário: Césio/análise
Césio/farmacocinética
Césio/farmacologia
Radioisótopos de Césio/farmacologia
Elementos
Alimentos
Espectrometria de Massas/métodos
Ocimum basilicum/efeitos dos fármacos
Ocimum basilicum/crescimento & desenvolvimento
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cesium Radioisotopes); 0 (Elements); 1KSV9V4Y4I (Cesium)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180214
[Lr] Data última revisão:
180214
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171013
[St] Status:MEDLINE


  3 / 4656 MEDLINE  
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[PMID]:28868786
[Au] Autor:Shukla S; Shukla S; Haur LJ; Dintakurti SSH; Han G; Priyadarshi A; Baikie T; Mhaisalkar SG; Mathews N
[Ad] Endereço:Energy Research Institute @ NTU (ERIAN), Nanyang Technological University, Singapore, Research Techno Plaza, 50 Nanyang Drive, 637553, Singapore.
[Ti] Título:Effect of Formamidinium/Cesium Substitution and PbI on the Long-Term Stability of Triple-Cation Perovskites.
[So] Source:ChemSusChem;10(19):3804-3809, 2017 Oct 09.
[Is] ISSN:1864-564X
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Altering cation and anion ratios in perovskites has proven an excellent means of tuning the perovskite properties and enhancing the performance. Recently, methylammonium/formamidinium/cesium triple-cation mixed-halide perovskites have demonstrated efficiencies up to 22 %. Similar to the widely explored methylammonium lead halide, excess PbI is added to these perovskite films to enhance their performances. The excess PbI is known to be beneficial for the performance. However, its impact on stability is less well known. Triple-cation perovskites deploy excess PbI up to 8 %. Thus, it is imperative to analyze the role of excess PbI in the degradation kinetics. In this study, the amount of PbI in the triple-cation perovskite films is varied and the degradation kinetics monitored by X-ray diffraction and optical absorption spectroscopy. The inclusion of excess PbI is shown to adversely affect the stability of the material. Faster degradation kinetics are observed for samples with higher PbI contents. However, samples with excess PbI also showed superior properties such as enhanced grain sizes and better optical absorption. Thus, careful management of the PbI quantity is required to obtain better stability and alternative pathways should be explored to achieve better device performance rather than adding excess PbI .
[Mh] Termos MeSH primário: Amidinas/química
Compostos de Cálcio/química
Césio/química
Fontes de Energia Elétrica
Iodetos/química
Chumbo/química
Óxidos/química
Energia Solar
Titânio/química
[Mh] Termos MeSH secundário: Flúor/química
Compostos de Estanho/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Amidines); 0 (Calcium Compounds); 0 (Iodides); 0 (Oxides); 0 (Tin Compounds); 12194-71-7 (perovskite); 1KSV9V4Y4I (Cesium); 284SYP0193 (Fluorine); 2P299V784P (Lead); 463-52-5 (formamidine); D1JT611TNE (Titanium); KM7N50LOS6 (stannic oxide)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171106
[Lr] Data última revisão:
171106
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170905
[St] Status:MEDLINE
[do] DOI:10.1002/cssc.201701203


  4 / 4656 MEDLINE  
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[PMID]:28785814
[Au] Autor:Nasukawa T; Uchiyama J; Taharaguchi S; Ota S; Ujihara T; Matsuzaki S; Murakami H; Mizukami K; Sakaguchi M
[Ad] Endereço:School of Veterinary Medicine, Azabu University, Fuchinobe 1-7-71, Chuo-ku, Sagamihara-shi, Kanagawa, 252-0206, Japan.
[Ti] Título:Virus purification by CsCl density gradient using general centrifugation.
[So] Source:Arch Virol;162(11):3523-3528, 2017 Nov.
[Is] ISSN:1432-8798
[Cp] País de publicação:Austria
[La] Idioma:eng
[Ab] Resumo:Virus purification by cesium chloride (CsCl) density gradient, which generally requires an expensive ultracentrifuge, is an essential technique in virology. Here, we optimized virus purification by CsCl density gradient using general centrifugation (40,000 × g, 2 h, 4 °C), which showed almost the same purification ability as conventional CsCl density gradient ultracentrifugation (100,000 × g, 1 h, 4 °C) using phages S13' and φEF24C. Moreover, adenovirus strain JM1/1 was also successfully purified by this method. We suggest that general centrifugation can become a less costly alternative to ultracentrifugation for virus purification by CsCl densiy gradient and will thus encourage research in virology.
[Mh] Termos MeSH primário: Bacteriófagos/classificação
Bacteriófagos/fisiologia
Centrifugação com Gradiente de Concentração/métodos
Césio/química
Cloretos/química
Virologia/métodos
[Mh] Termos MeSH secundário: Centrifugação com Gradiente de Concentração/instrumentação
Virologia/instrumentação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Chlorides); 1KSV9V4Y4I (Cesium); GNR9HML8BA (cesium chloride)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171025
[Lr] Data última revisão:
171025
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170809
[St] Status:MEDLINE
[do] DOI:10.1007/s00705-017-3513-z


  5 / 4656 MEDLINE  
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[PMID]:28778926
[Au] Autor:Renart ML; Montoya E; Giudici AM; Poveda JA; Fernández AM; Morales A; González-Ros JM
[Ad] Endereço:From the Instituto de Biología Molecular y Celular, Universidad Miguel Hernández, Elche, 03202 Alicante, Spain and.
[Ti] Título:Selective exclusion and selective binding both contribute to ion selectivity in KcsA, a model potassium channel.
[So] Source:J Biol Chem;292(37):15552-15560, 2017 Sep 15.
[Is] ISSN:1083-351X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The selectivity filter in potassium channels, a main component of the ion permeation pathway, configures a stack of binding sites (sites S1-S4) to which K and other cations may bind. Specific ion binding to such sites induces changes in the filter conformation, which play a key role in defining both selectivity and permeation. Here, using the potassium channel KcsA as a model, we contribute new evidence to reinforce this assertion. First, ion binding to KcsA blocked by tetrabutylammonium at the most cytoplasmic site in the selectivity filter (S4) suggests that such a site, when in the nonconductive filter conformation, has a higher affinity for cation binding than the most extracellular S1 site. This filter asymmetry, along with differences in intracellular and extracellular concentrations of K Na under physiological conditions, should strengthen selection of the permeant K by the channel. Second, we used different K concentrations to shift the equilibrium between nonconductive and conductive states of the selectivity filter in which to test competitive binding of Na These experiments disclosed a marked decrease in the affinity of Na to bind the channel when the conformational equilibrium shifts toward the conductive state. This finding suggested that in addition to the selective binding of K and other permeant species over Na , there is a selective exclusion of nonpermeant species from binding the channel filter, once it reaches a fully conductive conformation. We conclude that selective binding and selective exclusion of permeant and nonpermeant cations, respectively, are important determinants of ion channel selectivity.
[Mh] Termos MeSH primário: Proteínas de Bactérias/metabolismo
Modelos Moleculares
Canais de Potássio/metabolismo
Potássio/metabolismo
Streptomyces/metabolismo
[Mh] Termos MeSH secundário: Algoritmos
Substituição de Aminoácidos
Proteínas de Bactérias/química
Proteínas de Bactérias/genética
Sítios de Ligação
Ligação Competitiva
Césio/metabolismo
Detergentes/química
Detergentes/farmacologia
Glucosídeos/química
Glucosídeos/farmacologia
Temperatura Alta/efeitos adversos
Cinética
Mutação
Bloqueadores dos Canais de Potássio/química
Bloqueadores dos Canais de Potássio/farmacologia
Canais de Potássio/química
Canais de Potássio/genética
Desnaturação Proteica
Estabilidade Proteica
Compostos de Amônio Quaternário/química
Compostos de Amônio Quaternário/farmacologia
Proteínas Recombinantes de Fusão/química
Proteínas Recombinantes de Fusão/metabolismo
Rubídio/metabolismo
Sódio/metabolismo
Solubilidade
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Detergents); 0 (Glucosides); 0 (Potassium Channel Blockers); 0 (Potassium Channels); 0 (Quaternary Ammonium Compounds); 0 (Recombinant Fusion Proteins); 0 (prokaryotic potassium channel); 1KSV9V4Y4I (Cesium); 69227-93-6 (dodecyl maltoside); 9NEZ333N27 (Sodium); CBU2X6BBJR (tetrabutylammonium); MLT4718TJW (Rubidium); RWP5GA015D (Potassium)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170927
[Lr] Data última revisão:
170927
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170806
[St] Status:MEDLINE
[do] DOI:10.1074/jbc.M117.795807


  6 / 4656 MEDLINE  
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[PMID]:28658059
[Au] Autor:Frank SJ; Kearfott KJ
[Ad] Endereço:*University of Michigan, Department of Nuclear Engineering and Radiological Sciences, Ann Arbor, MI 48109-2104.
[Ti] Título:Imaging of Gamma-ray Scatter from a Polymethyl-methacrylate Phantom Using a Compton Imaging Spectrometer.
[So] Source:Health Phys;113(2):135-142, 2017 Aug.
[Is] ISSN:1538-5159
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Commercially available gamma-ray imaging spectrometers have been introduced recently and are currently undergoing investigations for various applications in nuclear power plants, environmental management, and medical environments. A Compton imaging gamma-ray spectrometer uses an array of detectors or a single position-sensitive crystal to create planar images of radionuclide distributions. The typical software included with these devices creates images of specific radionuclides using only the counts under their known gamma emission photopeaks. This approach prevents the direct imaging of scattered radiation, which is of interest for many radiation protection applications. In this paper, a technique for imaging radiation scatter or portions of the scatter spectrum is implemented. This involves the creation of a virtual radionuclide in software with peaks placed throughout the backscatter continuum of interest and then imaging that virtual radionuclide in the post-processing software. This technique is used to image the Compton scatter successfully from a polymethyl-methacrylate (PMMA) phantom placed in a Cs irradiator beam. Measured scatter energies were found to be within 15% of the expected values, sufficient to predict scatter behavior and individually measure separate sources of scatter at different angles.
[Mh] Termos MeSH primário: Materiais Biomiméticos/química
Césio/análise
Raios gama
Imagens de Fantasmas
Polimetil Metacrilato/química
Espectrometria gama/instrumentação
[Mh] Termos MeSH secundário: Desenho de Equipamento
Análise de Falha de Equipamento
Seres Humanos
Dose de Radiação
Reprodutibilidade dos Testes
Sensibilidade e Especificidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
1KSV9V4Y4I (Cesium); 9011-14-7 (Polymethyl Methacrylate)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170818
[Lr] Data última revisão:
170818
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170629
[St] Status:MEDLINE
[do] DOI:10.1097/HP.0000000000000681


  7 / 4656 MEDLINE  
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[PMID]:28591601
[Au] Autor:Siebenmorgen T; Zacharias M
[Ad] Endereço:Physics Department, Technical University of Munich, Garching, Germany.
[Ti] Título:Origin of Ion Specificity of Telomeric DNA G-Quadruplexes Investigated by Free-Energy Simulations.
[So] Source:Biophys J;112(11):2280-2290, 2017 Jun 06.
[Is] ISSN:1542-0086
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Telomeric DNA consists of tandem repeats of the sequence d(TTAGGG) that form G-quadruplex structures made of stacked guanines with monovalent cations bound at a central cavity. Although different ions can stabilize a G-quadruplex structure, the preferred bound ions are typically K or Na . Several different strand-folding topologies have been reported for Q-quadruplexes formed from telomeric repeats depending on DNA length and ion solution condition. This suggests a possible dependence of the ion selectivity of the central pore on the folding topology of the quadruplex. Molecular dynamics free energy perturbation has been employed to systematically study the relative affinity of the central quadruplex pore for different cation types and the associated energetic and solvation contributions to ion selectivity. The calculations have been performed on two different common quadruplex folding topologies. For both topologies, the same ion selectivity was found with a preference for K followed by Rb and Na , which agrees with the experimentally determined preference for most investigated quadruplexes. The selectivity is determined by a balance between attractive Coulomb interactions and loss of hydration but also modulated by van der Waals contributions. Specificity is mediated by the central guanines and no significant contribution of the nucleic acid backbone. The simulations indicate that different topologies might be stabilized by ions bound at the surface or alternative sites of the quadruplex because the ion specificity of the central pore does not depend on the strand folding topology.
[Mh] Termos MeSH primário: Cátions/metabolismo
Quadruplex G
Telômero/metabolismo
[Mh] Termos MeSH secundário: Cátions/química
Césio/química
Césio/metabolismo
Guanina/química
Guanina/metabolismo
Lítio/química
Lítio/metabolismo
Simulação de Dinâmica Molecular
Potássio/química
Potássio/metabolismo
Rubídio/química
Rubídio/metabolismo
Sódio/química
Sódio/metabolismo
Soluções
Telômero/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cations); 0 (Solutions); 1KSV9V4Y4I (Cesium); 5Z93L87A1R (Guanine); 9FN79X2M3F (Lithium); 9NEZ333N27 (Sodium); MLT4718TJW (Rubidium); RWP5GA015D (Potassium)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170814
[Lr] Data última revisão:
170814
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170608
[St] Status:MEDLINE


  8 / 4656 MEDLINE  
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[PMID]:28571846
[Au] Autor:Zheng X; Dou J; Yuan J; Qin W; Hong X; Ding A
[Ad] Endereço:College of Water Sciences, Beijing Normal University, Beijing 100875, China. Electronic address: zhengxianming1@163.com.
[Ti] Título:Removal of Cs from water and soil by ammonium-pillared montmorillonite/Fe O composite.
[So] Source:J Environ Sci (China);56:12-24, 2017 Jun.
[Is] ISSN:1001-0742
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:To remove cesium ions from water and soil, a novel adsorbent was synthesized by following a one-step co-precipitation method and using non-toxic raw materials. By combining ammonium-pillared montmorillonite (MMT) and magnetic nanoparticles (Fe O ), an MMT/Fe O composite was prepared and characterized. The adsorbent exhibited high selectivity of Cs and could be rapidly separated from the mixed solution under an external magnetic field. Above all, the adsorbent had high removal efficiency in cesium-contaminated samples (water and soil) and also showed good recycling performance, indicating that the MMT/Fe O composite could be widely applied to the remediation of cesium-contaminated environments. It was observed that the pH, solid/liquid ratio and initial concentration affected adsorption capacity. In the presence of coexisting ions, the adsorption capacity decreased in the order of Ca >Mg >K >Na , which is consistent with our theoretical prediction. The adsorption behavior of this new adsorbent could be expressed by the pseudo-second-order model and Freundlich isotherm. In addition, the adsorption mechanism of Cs was NH ion exchange and surface hydroxyl group coordination, with the former being more predominant.
[Mh] Termos MeSH primário: Compostos de Amônio/química
Césio/análise
Poluentes Químicos da Água/análise
[Mh] Termos MeSH secundário: Adsorção
Bentonita/química
Césio/química
Precipitação Química
Concentração de Íons de Hidrogênio
Cinética
Solo/química
Água/química
Purificação da Água/métodos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Ammonium Compounds); 0 (Soil); 0 (Water Pollutants, Chemical); 059QF0KO0R (Water); 1302-78-9 (Bentonite); 1KSV9V4Y4I (Cesium)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170603
[St] Status:MEDLINE


  9 / 4656 MEDLINE  
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[PMID]:28569231
[Au] Autor:Wang R; Chu Y; Chen M
[Ti] Título:Adsorption Kinetics of Cs / Sr on Ca-Bentonite.
[So] Source:Water Environ Res;89(9):791-797, 2017 Sep 01.
[Is] ISSN:1061-4303
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:137Cs+/90Sr2+ adsorption kinetics on natural Ca-bentonite under the impact of different adsorption conditions was examined in detail. The results indicate that natural Ca-bentonite shows a strong adsorption capacity for Cs+/Sr2+. The adsorption reached at equilibrium after 2 hours for Cs+. Cs+/Sr2+removal efficiency reaches the highest when the pH value is 5 to 8 and increases with the increase of Ca-bentonite adding amount. Cs+ removal efficiency increases with the increase of Cs+ initial concentration, while Sr2+ removal efficiency slightly varies around 70%. The adsorption of Cs+/Sr2+ by Ca-bentonite follows the pseudo-second-order model and is controlled by chemical adsorption. Meanwhile, the results of intraparticle diffusion modelling indicate that intraparticle diffusion is a kinetics controlling step, besides surface adsorption and liquid film diffusion.
[Mh] Termos MeSH primário: Bentonita/química
Cálcio/química
Césio/química
Estrôncio/química
Poluentes Radioativos da Água/química
[Mh] Termos MeSH secundário: Adsorção
Radioisótopos de Césio/química
Radioisótopos de Estrôncio/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cesium Radioisotopes); 0 (Strontium Radioisotopes); 0 (Water Pollutants, Radioactive); 1302-78-9 (Bentonite); 1KSV9V4Y4I (Cesium); SY7Q814VUP (Calcium); YZS2RPE8LE (Strontium)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171103
[Lr] Data última revisão:
171103
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170602
[St] Status:MEDLINE
[do] DOI:10.2175/106143017X14902968254593


  10 / 4656 MEDLINE  
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[PMID]:28423006
[Au] Autor:Kruse CPS; Basu P; Luesse DR; Wyatt SE
[Ad] Endereço:Department of Environmental and Plant Biology, Ohio University, Athens, OH, United States of America.
[Ti] Título:Transcriptome and proteome responses in RNAlater preserved tissue of Arabidopsis thaliana.
[So] Source:PLoS One;12(4):e0175943, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Tissue preservation is a minimal requirement for the success of plant RNA and protein expression studies. The standard of snap-freezing in liquid nitrogen is not always practical or possible. RNAlater, a concentrated solution of ammonium and cesium sulfates, has become a standard preservative in the absence of liquid nitrogen. Here, we demonstrate the effectiveness of RNAlater in preserving both RNA and proteins in Arabidopsis thaliana tissues for use in RNAseq and LC-MS/MS analysis of proteins. While successful in preserving plant material, a transcriptomic and proteomic response is evident. Specifically, 5770 gene transcripts, 84 soluble proteins, and 120 membrane-bound proteins were found to be differentially expressed at a log-fold change of ±1 (P ≤ 0.05). This response is mirrored in the abundance of post-translational modifications, with 23 of the 108 (21.3%) phosphorylated proteins showing altered abundance at a log-fold change of ±1 (P ≤ 0.05). While RNAlater is effective in preserving biological information, our findings warrant caution in its use for transcriptomic and proteomic experiments.
[Mh] Termos MeSH primário: Proteínas de Arabidopsis/genética
Arabidopsis/genética
Regulação da Expressão Gênica de Plantas
Processamento de Proteína Pós-Traducional
RNA de Plantas/genética
Preservação de Tecido/métodos
Transcriptoma
[Mh] Termos MeSH secundário: Sulfato de Amônio/química
Césio/química
Cromatografia Líquida
Fixadores/química
Perfilação da Expressão Gênica
Sequenciamento de Nucleotídeos em Larga Escala
Oxirredução
Fosforilação
Espectrometria de Massas em Tandem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Arabidopsis Proteins); 0 (Fixatives); 0 (RNA, Plant); 1KSV9V4Y4I (Cesium); 8D6R91CS62 (cesium sulfate); SU46BAM238 (Ammonium Sulfate)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170506
[Lr] Data última revisão:
170506
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170420
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0175943



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