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[PMID]:28778926
[Au] Autor:Renart ML; Montoya E; Giudici AM; Poveda JA; Fernández AM; Morales A; González-Ros JM
[Ad] Endereço:From the Instituto de Biología Molecular y Celular, Universidad Miguel Hernández, Elche, 03202 Alicante, Spain and.
[Ti] Título:Selective exclusion and selective binding both contribute to ion selectivity in KcsA, a model potassium channel.
[So] Source:J Biol Chem;292(37):15552-15560, 2017 Sep 15.
[Is] ISSN:1083-351X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The selectivity filter in potassium channels, a main component of the ion permeation pathway, configures a stack of binding sites (sites S1-S4) to which K and other cations may bind. Specific ion binding to such sites induces changes in the filter conformation, which play a key role in defining both selectivity and permeation. Here, using the potassium channel KcsA as a model, we contribute new evidence to reinforce this assertion. First, ion binding to KcsA blocked by tetrabutylammonium at the most cytoplasmic site in the selectivity filter (S4) suggests that such a site, when in the nonconductive filter conformation, has a higher affinity for cation binding than the most extracellular S1 site. This filter asymmetry, along with differences in intracellular and extracellular concentrations of K Na under physiological conditions, should strengthen selection of the permeant K by the channel. Second, we used different K concentrations to shift the equilibrium between nonconductive and conductive states of the selectivity filter in which to test competitive binding of Na These experiments disclosed a marked decrease in the affinity of Na to bind the channel when the conformational equilibrium shifts toward the conductive state. This finding suggested that in addition to the selective binding of K and other permeant species over Na , there is a selective exclusion of nonpermeant species from binding the channel filter, once it reaches a fully conductive conformation. We conclude that selective binding and selective exclusion of permeant and nonpermeant cations, respectively, are important determinants of ion channel selectivity.
[Mh] Termos MeSH primário: Proteínas de Bactérias/metabolismo
Modelos Moleculares
Canais de Potássio/metabolismo
Potássio/metabolismo
Streptomyces/metabolismo
[Mh] Termos MeSH secundário: Algoritmos
Substituição de Aminoácidos
Proteínas de Bactérias/química
Proteínas de Bactérias/genética
Sítios de Ligação
Ligação Competitiva
Césio/metabolismo
Detergentes/química
Detergentes/farmacologia
Glucosídeos/química
Glucosídeos/farmacologia
Temperatura Alta/efeitos adversos
Cinética
Mutação
Bloqueadores dos Canais de Potássio/química
Bloqueadores dos Canais de Potássio/farmacologia
Canais de Potássio/química
Canais de Potássio/genética
Desnaturação Proteica
Estabilidade Proteica
Compostos de Amônio Quaternário/química
Compostos de Amônio Quaternário/farmacologia
Proteínas Recombinantes de Fusão/química
Proteínas Recombinantes de Fusão/metabolismo
Rubídio/metabolismo
Sódio/metabolismo
Solubilidade
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Detergents); 0 (Glucosides); 0 (Potassium Channel Blockers); 0 (Potassium Channels); 0 (Quaternary Ammonium Compounds); 0 (Recombinant Fusion Proteins); 0 (prokaryotic potassium channel); 1KSV9V4Y4I (Cesium); 69227-93-6 (dodecyl maltoside); 9NEZ333N27 (Sodium); CBU2X6BBJR (tetrabutylammonium); MLT4718TJW (Rubidium); RWP5GA015D (Potassium)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170927
[Lr] Data última revisão:
170927
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170806
[St] Status:MEDLINE
[do] DOI:10.1074/jbc.M117.795807


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[PMID]:28675845
[Au] Autor:Naidu G; Jeong S; Johir MAH; Fane AG; Kandasamy J; Vigneswaran S
[Ad] Endereço:Faculty of Engineering, University of Technology Sydney (UTS), P.O. Box 123, Broadway, NSW 2007 Australia.
[Ti] Título:Rubidium extraction from seawater brine by an integrated membrane distillation-selective sorption system.
[So] Source:Water Res;123:321-331, 2017 Oct 15.
[Is] ISSN:1879-2448
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The ultimate goal of seawater reverse osmosis (SWRO) brine management is to achieve minimal liquid discharge while recovering valuable resources. The suitability of an integrated system of membrane distillation (MD) with sorption for the recovery of rubidium (Rb ) and simultaneous SWRO brine volume reduction has been evaluated for the first time. Polymer encapsulated potassium copper hexacyanoferrate (KCuFC(PAN)) sorbent exhibited a good selectivity for Rb sorption with 10-15% increment at 55 °C (Langmuir Q = 125.11 ± 0.20 mg/g) compared to at 25 °C (Langmuir Q = 108.71 ± 0.20 mg/g). The integrated MD-KCuFC(PAN) system with periodic membrane cleaning, enabled concentration of SWRO brine to a volume concentration factor (VCF) of 2.9 (65% water recovery). A stable MD permeate flux was achieved with good quality permeate (conductivity of 15-20 µS/cm). Repeated cycles of MD-KCuFC(PAN) sorption with SWRO brine enabled the extraction of 2.26 mg Rb from 12 L of brine (equivalent to 1.9 kg of Rb/day, or 0.7 tonne/yr from a plant producing 10,000 m /day brine). KCuFC(PAN) showed a high regeneration and reuse capacity. NH Cl air stripping followed by resorcinol formaldehyde (RF) resin filtration enabled to recover Rb from the desorbed solution.
[Mh] Termos MeSH primário: Rubídio/química
Purificação da Água
[Mh] Termos MeSH secundário: Destilação
Membranas Artificiais
Osmose
Sais
Água do Mar
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Membranes, Artificial); 0 (Salts); 0 (brine); MLT4718TJW (Rubidium)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171013
[Lr] Data última revisão:
171013
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170705
[St] Status:MEDLINE


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[PMID]:28591601
[Au] Autor:Siebenmorgen T; Zacharias M
[Ad] Endereço:Physics Department, Technical University of Munich, Garching, Germany.
[Ti] Título:Origin of Ion Specificity of Telomeric DNA G-Quadruplexes Investigated by Free-Energy Simulations.
[So] Source:Biophys J;112(11):2280-2290, 2017 Jun 06.
[Is] ISSN:1542-0086
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Telomeric DNA consists of tandem repeats of the sequence d(TTAGGG) that form G-quadruplex structures made of stacked guanines with monovalent cations bound at a central cavity. Although different ions can stabilize a G-quadruplex structure, the preferred bound ions are typically K or Na . Several different strand-folding topologies have been reported for Q-quadruplexes formed from telomeric repeats depending on DNA length and ion solution condition. This suggests a possible dependence of the ion selectivity of the central pore on the folding topology of the quadruplex. Molecular dynamics free energy perturbation has been employed to systematically study the relative affinity of the central quadruplex pore for different cation types and the associated energetic and solvation contributions to ion selectivity. The calculations have been performed on two different common quadruplex folding topologies. For both topologies, the same ion selectivity was found with a preference for K followed by Rb and Na , which agrees with the experimentally determined preference for most investigated quadruplexes. The selectivity is determined by a balance between attractive Coulomb interactions and loss of hydration but also modulated by van der Waals contributions. Specificity is mediated by the central guanines and no significant contribution of the nucleic acid backbone. The simulations indicate that different topologies might be stabilized by ions bound at the surface or alternative sites of the quadruplex because the ion specificity of the central pore does not depend on the strand folding topology.
[Mh] Termos MeSH primário: Cátions/metabolismo
Quadruplex G
Telômero/metabolismo
[Mh] Termos MeSH secundário: Cátions/química
Césio/química
Césio/metabolismo
Guanina/química
Guanina/metabolismo
Lítio/química
Lítio/metabolismo
Simulação de Dinâmica Molecular
Potássio/química
Potássio/metabolismo
Rubídio/química
Rubídio/metabolismo
Sódio/química
Sódio/metabolismo
Soluções
Telômero/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cations); 0 (Solutions); 1KSV9V4Y4I (Cesium); 5Z93L87A1R (Guanine); 9FN79X2M3F (Lithium); 9NEZ333N27 (Sodium); MLT4718TJW (Rubidium); RWP5GA015D (Potassium)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170814
[Lr] Data última revisão:
170814
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170608
[St] Status:MEDLINE


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[PMID]:28293274
[Au] Autor:Ocneanu AF; deKemp RA; Renaud JM; Adler A; Beanlands RS; Klein R
[Ad] Endereço:Systems and Computer Engineering, Carleton University, Ottawa, ON, Canada.
[Ti] Título:Optimally Repeatable Kinetic Model Variant for Myocardial Blood Flow Measurements with Rb PET.
[So] Source:Comput Math Methods Med;2017:6810626, 2017.
[Is] ISSN:1748-6718
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Myocardial blood flow (MBF) quantification with Rb positron emission tomography (PET) is gaining clinical adoption, but improvements in precision are desired. This study aims to identify analysis variants producing the most repeatable MBF measures. 12 volunteers underwent same-day test-retest rest and dipyridamole stress imaging with dynamic Rb PET, from which MBF was quantified using 1-tissue-compartment kinetic model variants: (1) blood-pool versus uptake region sampled input function (Blood/Uptake-ROI), (2) dual spillover correction (SOC-On/Off), (3) right blood correction (RBC-On/Off), (4) arterial blood transit delay (Delay-On/Off), and (5) distribution volume (DV) constraint (Global/Regional-DV). Repeatability of MBF, stress/rest myocardial flow reserve (MFR), and stress/rest MBF difference (ΔMBF) was assessed using nonparametric reproducibility coefficients (RPC = 1.45 × interquartile range). MBF using SOC-On, RVBC-Off, Blood-ROI, Global-DV, and Delay-Off was most repeatable for combined rest and stress: RPC = 0.21 mL/min/g (15.8%). Corresponding MFR and ΔMBF RPC were 0.42 (20.2%) and 0.24 mL/min/g (23.5%). MBF repeatability improved with SOC-On at stress ( < 0.001) and tended to improve with RBC-Off at both rest and stress ( < 0.08). DV and ROI did not significantly influence repeatability. The Delay-On model was overdetermined and did not reliably converge. MBF and MFR test-retest repeatability were the best with dual spillover correction, left atrium blood input function, and global DV.
[Mh] Termos MeSH primário: Processamento de Imagem Assistida por Computador/métodos
Tomografia por Emissão de Pósitrons
Rubídio/química
[Mh] Termos MeSH secundário: Adulto
Idoso
Idoso de 80 Anos ou mais
Algoritmos
Estudos de Coortes
Doença da Artéria Coronariana/diagnóstico por imagem
Doença da Artéria Coronariana/fisiopatologia
Circulação Coronária
Dipiridamol/química
Feminino
Hemodinâmica
Seres Humanos
Cinética
Masculino
Meia-Idade
Imagem de Perfusão do Miocárdio
Radioisótopos
Reprodutibilidade dos Testes
Tomografia Computadorizada por Raios X
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Radioisotopes); 64ALC7F90C (Dipyridamole); MLT4718TJW (Rubidium)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170403
[Lr] Data última revisão:
170403
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170316
[St] Status:MEDLINE
[do] DOI:10.1155/2017/6810626


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[PMID]:28185866
[Au] Autor:Ren J; Lee H; Yoo SM; Yu MS; Park H; Na D
[Ad] Endereço:School of Integrative Engineering, Chung-Ang University, 84 Heukseok-ro, Dongjak-gu, Seoul 06974, Republic of Korea.
[Ti] Título:Combined chemical and physical transformation method with RbCl and sepiolite for the transformation of various bacterial species.
[So] Source:J Microbiol Methods;135:48-51, 2017 Apr.
[Is] ISSN:1872-8359
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:DNA transformation that delivers plasmid DNAs into bacterial cells is fundamental in genetic manipulation to engineer and study bacteria. Developed transformation methods to date are optimized to specific bacterial species for high efficiency. Thus, there is always a demand for simple and species-independent transformation methods. We herein describe the development of a chemico-physical transformation method that combines a rubidium chloride (RbCl)-based chemical method and sepiolite-based physical method, and report its use for the simple and efficient delivery of DNA into various bacterial species. Using this method, the best transformation efficiency for Escherichia coli DH5α was 4.3×10 CFU/µg of pUC19 plasmid, which is higher than or comparable to the reported transformation efficiencies to date. This method also allowed the introduction of plasmid DNAs into Bacillus subtilis (5.7×10 CFU/µg of pSEVA3b67Rb), Bacillus megaterium (2.5×10 CFU/µg of pSPAsp-hp), Lactococcus lactis subsp. lactis (1.0×10 CFU/µg of pTRKH3-ermGFP), and Lactococcus lactis subsp. cremoris (2.2×10 CFU/µg of pMSP3535VA). Remarkably, even when the conventional chemical and physical methods failed to generate transformed cells in Bacillus sp. and Enterococcus faecalis, E. malodoratus and E. mundtii, our combined method showed a significant transformation efficiency (2.4×10 , 4.5×10 , 2×10 , and 0.5×10 CFU/µg of plasmid DNA). Based on our results, we anticipate that our simple and efficient transformation method should prove usefulness for introducing DNA into various bacterial species without complicated optimization of parameters affecting DNA entry into the cell.
[Mh] Termos MeSH primário: Bactérias/genética
Cloretos/farmacologia
DNA Bacteriano/genética
Técnicas de Transferência de Genes
Silicatos de Magnésio/farmacologia
Rubídio/farmacologia
Transformação Bacteriana/efeitos dos fármacos
[Mh] Termos MeSH secundário: Bacillus/genética
Bacillus megaterium/genética
Bacillus subtilis/genética
Enterococcus faecalis/genética
Escherichia coli/genética
Lactococcus lactis
Plasmídeos/genética
Transformação Bacteriana/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Chlorides); 0 (DNA, Bacterial); 0 (Magnesium Silicates); C2E1CI501T (magnesium trisilicate); MLT4718TJW (Rubidium); N3SHC5273S (rubidium chloride)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171002
[Lr] Data última revisão:
171002
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170211
[St] Status:MEDLINE


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[PMID]:28146214
[Au] Autor:Padilha BG; Sabino D; Giorgi MC; Soares J; Izaki M; Meneghetti JC
[Ad] Endereço:Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo, São Paulo, SP, Brazil.
[Ti] Título:Case Report: Multivessel Coronary Disease Assessment with SPECT 99mTc-Sestamibi and Rubidium-82 PET/CT.
[So] Source:Arq Bras Cardiol;108(1):87-90, 2017 Jan.
[Is] ISSN:1678-4170
[Cp] País de publicação:Brazil
[La] Idioma:por; eng
[Mh] Termos MeSH primário: Doença da Artéria Coronariana/diagnóstico por imagem
Imagem de Perfusão do Miocárdio/métodos
Tomografia Computadorizada com Tomografia por Emissão de Pósitrons/métodos
Compostos Radiofarmacêuticos
Rubídio
Tecnécio Tc 99m Sestamibi
[Mh] Termos MeSH secundário: Angiografia Coronária/métodos
Doença da Artéria Coronariana/fisiopatologia
Feminino
Reserva Fracionada de Fluxo Miocárdico
Seres Humanos
Meia-Idade
Reprodutibilidade dos Testes
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Radiopharmaceuticals); 971Z4W1S09 (Technetium Tc 99m Sestamibi); MLT4718TJW (Rubidium)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170202
[St] Status:MEDLINE


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[PMID]:27939886
[Au] Autor:Mousa JJ; Newsome RC; Yang Y; Jobin C; Bruner SD
[Ad] Endereço:Department of Chemistry, University of Florida, Gainesville, FL, 32611, USA.
[Ti] Título:ClbM is a versatile, cation-promiscuous MATE transporter found in the colibactin biosynthetic gene cluster.
[So] Source:Biochem Biophys Res Commun;482(4):1233-1239, 2017 Jan 22.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Multidrug transporters play key roles in cellular drug resistance to toxic molecules, yet these transporters are also involved in natural product transport as part of biosynthetic clusters in bacteria and fungi. The genotoxic molecule colibactin is produced by strains of virulent and pathobiont Escherichia coli and Klebsiella pneumoniae. In the biosynthetic cluster is a multidrug and toxic compound extrusion protein (MATE) proposed to transport the prodrug molecule precolibactin across the cytoplasmic membrane, for subsequent cleavage by the peptidase ClbP and cellular export. We recently determined the X-ray structure of ClbM, and showed preliminary data suggesting its specific role in precolibactin transport. Here, we define a functional role of ClbM by examining transport capabilities under various biochemical conditions. Our data indicate ClbM responds to sodium, potassium, and rubidium ion gradients, while also having substantial transport activity in the absence of alkali cations.
[Mh] Termos MeSH primário: Proteínas de Escherichia coli/genética
Escherichia coli/genética
Proteínas de Transporte de Cátions Orgânicos/genética
Peptídeos/genética
Peptídeos/metabolismo
Policetídeos/metabolismo
[Mh] Termos MeSH secundário: Antiporters/metabolismo
Proteínas de Bactérias/metabolismo
Produtos Biológicos/química
Transporte Biológico
Transporte Biológico Ativo
Cátions
Cristalografia por Raios X
Citoplasma/metabolismo
Escherichia coli/metabolismo
Proteínas de Escherichia coli/metabolismo
Ligações de Hidrogênio
Concentração de Íons de Hidrogênio
Transporte de Íons
Klebsiella pneumoniae/metabolismo
Microbiota
Família Multigênica
Mutação
Proteínas de Transporte de Cátions Orgânicos/metabolismo
Peptídeo Hidrolases/metabolismo
Potássio/química
Pró-Fármacos
Estrutura Secundária de Proteína
Rodaminas/química
Rubídio/química
Sódio/química
Água/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antiporters); 0 (Bacterial Proteins); 0 (Biological Products); 0 (Cations); 0 (ClbM protein, E coli); 0 (Escherichia coli Proteins); 0 (Organic Cation Transport Proteins); 0 (Peptides); 0 (Polyketides); 0 (Prodrugs); 0 (Rhodamines); 0 (colibactin); 037VRW83CF (rhodamine 6G); 059QF0KO0R (Water); 9NEZ333N27 (Sodium); EC 3.4.- (ClbP protein, E coli); EC 3.4.- (Peptide Hydrolases); MLT4718TJW (Rubidium); RWP5GA015D (Potassium)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170606
[Lr] Data última revisão:
170606
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161213
[St] Status:MEDLINE


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[PMID]:27748508
[Au] Autor:Shahidullah M; Mandal A; Delamere NA
[Ad] Endereço:Department of Physiology and Ophthalmology and Vision Science, University of Arizona, Tucson, Arizona.
[Ti] Título:Src Family Kinase Links Insulin Signaling to Short Term Regulation of Na,K-ATPase in Nonpigmented Ciliary Epithelium.
[So] Source:J Cell Physiol;232(6):1489-1500, 2017 Jun.
[Is] ISSN:1097-4652
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Insulin has been shown to elicit changes of Na,K-ATPase activity in various tissues. Na,K-ATPase in the nonpigmented ciliary epithelium (NPE) plays a role in aqueous humor secretion and changes of Na,K-ATPase activity impact the driving force. Because we detect a change of NPE Na,K-ATPase activity in response to insulin, studies were carried out to examine the response mechanism. Ouabain-sensitive rubidium (Rb) uptake by cultured NPE cells, measured as a functional index of Na,K-ATPase-mediated inward potassium transport, was found to increase in cells exposed for 5 min to insulin. The maximally effective concentration was 100 nM. An intrinsic increase of Na,K-ATPase activity evident as a >2-fold increase in the rate of ouabain-sensitive ATP hydrolysis in homogenates obtained from cells exposed to 100 nM insulin for 5 min was also observed. Insulin-treated cells exhibited Akt, Src family kinase (SFK), ERK1/2, and p38 activation, all of which were prevented by a pI3 kinase inhibitor LY294002. The Rb uptake and Na,K-ATPase activity response to insulin both were abolished by PP2, an SFK inhibitor which also prevented p38 and ERK1/2 but not Akt activation. The Akt inhibitor MK-2206 did not change the Na,K-ATPase response to insulin. The findings suggest insulin activates pI3K-dependent Akt and SFK signaling pathways that are separate. ERK1/2 and p38 activation is secondary to and dependent on SFK activation. The increase of Na,K-ATPase activity is dependent on activation of the SFK pathway. The findings are consistent with previous studies that indicate a link between Na,K-ATPase activity and SFK signaling. J. Cell. Physiol. 232: 1489-1500, 2017. © 2016 Wiley Periodicals, Inc.
[Mh] Termos MeSH primário: Corpo Ciliar/metabolismo
Epitélio/metabolismo
Insulina/metabolismo
Pigmentação
Transdução de Sinais
ATPase Trocadora de Sódio-Potássio/metabolismo
Quinases da Família src/metabolismo
[Mh] Termos MeSH secundário: Animais
Butadienos/farmacologia
Cromonas/farmacologia
Corpo Ciliar/efeitos dos fármacos
Ativação Enzimática/efeitos dos fármacos
Epitélio/efeitos dos fármacos
MAP Quinases Reguladas por Sinal Extracelular/metabolismo
Compostos Heterocíclicos com 3 Anéis/farmacologia
Imidazóis/farmacologia
Insulina/farmacologia
Modelos Biológicos
Morfolinas/farmacologia
Nitrilos/farmacologia
Ouabaína/farmacologia
Fosfatidilinositol 3-Quinases/antagonistas & inibidores
Fosfatidilinositol 3-Quinases/metabolismo
Fosforilação/efeitos dos fármacos
Pigmentação/efeitos dos fármacos
Proteínas Proto-Oncogênicas c-akt/metabolismo
Piridinas/farmacologia
Pirimidinas/farmacologia
Rubídio/metabolismo
Transdução de Sinais/efeitos dos fármacos
Sus scrofa
Fatores de Tempo
Proteínas Quinases p38 Ativadas por Mitógeno/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (AG 1879); 0 (Butadienes); 0 (Chromones); 0 (Heterocyclic Compounds, 3-Ring); 0 (Imidazoles); 0 (Insulin); 0 (MK 2206); 0 (Morpholines); 0 (Nitriles); 0 (Pyridines); 0 (Pyrimidines); 0 (U 0126); 31M2U1DVID (2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one); 5ACL011P69 (Ouabain); EC 2.7.1.- (Phosphatidylinositol 3-Kinases); EC 2.7.10.2 (src-Family Kinases); EC 2.7.11.1 (Proto-Oncogene Proteins c-akt); EC 2.7.11.24 (Extracellular Signal-Regulated MAP Kinases); EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases); EC 3.6.3.9 (Sodium-Potassium-Exchanging ATPase); MLT4718TJW (Rubidium); PVX798P8GI (4-(4-fluorophenyl)-2-(4-hydroxyphenyl)-5-(4-pyridyl)imidazole)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170515
[Lr] Data última revisão:
170515
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161018
[St] Status:MEDLINE
[do] DOI:10.1002/jcp.25654


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[PMID]:27842602
[Au] Autor:Roberts BR; Doecke JD; Rembach A; Yévenes LF; Fowler CJ; McLean CA; Lind M; Volitakis I; Masters CL; Bush AI; Hare DJ; AIBL research group
[Ad] Endereço:The Florey Institute of Neuroscience, The University of Melbourne, Parkville, VIC, Australia. blaine.roberts@florey.edu.au.
[Ti] Título:Rubidium and potassium levels are altered in Alzheimer's disease brain and blood but not in cerebrospinal fluid.
[So] Source:Acta Neuropathol Commun;4(1):119, 2016 Nov 14.
[Is] ISSN:2051-5960
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Loss of intracellular compartmentalization of potassium is a biochemical feature of Alzheimer's disease indicating a loss of membrane integrity and mitochondrial dysfunction. We examined potassium and rubidium (a biological proxy for potassium) in brain tissue, blood fractions and cerebrospinal fluid from Alzheimer's disease and healthy control subjects to investigate the diagnostic potential of these two metal ions. We found that both potassium and rubidium levels were significantly decreased across all intracellular compartments in the Alzheimer's disease brain. Serum from over 1000 participants in the Australian Imaging, Biomarkers and Lifestyle Flagship Study of Ageing (AIBL), showed minor changes according to disease state. Potassium and rubidium levels in erythrocytes and cerebrospinal fluid were not significantly different according to disease state, and rubidium was slightly decreased in Alzheimer's disease patients compared to healthy controls. Our data provides evidence that contrasts the hypothesized disruption of the blood-brain barrier in Alzheimer's disease, with the systemic decrease in cortical potassium and rubidium levels suggesting influx of ions from the blood is minimal and that the observed changes are more likely indicative of an internal energy crisis within the brain. These findings may be the basis for potential diagnostic imaging studies using radioactive potassium and rubidium tracers.
[Mh] Termos MeSH primário: Doença de Alzheimer/metabolismo
Plaquetas/metabolismo
Encéfalo/metabolismo
Eritrócitos/metabolismo
Potássio/metabolismo
Rubídio/metabolismo
[Mh] Termos MeSH secundário: Idoso
Idoso de 80 Anos ou mais
Doença de Alzheimer/genética
Análise de Variância
Apolipoproteína E4/genética
Austrália
Biomarcadores/metabolismo
Feminino
Seres Humanos
Masculino
Punção Espinal
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Apolipoprotein E4); 0 (Biomarkers); MLT4718TJW (Rubidium); RWP5GA015D (Potassium)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171024
[Lr] Data última revisão:
171024
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161116
[St] Status:MEDLINE


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[PMID]:27413034
[Au] Autor:Srivaratharajah K; Coutinho T; deKemp R; Liu P; Haddad H; Stadnick E; Davies RA; Chih S; Dwivedi G; Guo A; Wells GA; Bernick J; Beanlands R; Mielniczuk LM
[Ad] Endereço:From the Division of Cardiology, University of Ottawa Heart Institute, Ontario, Canada.
[Ti] Título:Reduced Myocardial Flow in Heart Failure Patients With Preserved Ejection Fraction.
[So] Source:Circ Heart Fail;9(7), 2016 Jul.
[Is] ISSN:1941-3297
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: There remains limited insight into the pathophysiology and therapeutic advances directed at improving prognosis for patients with heart failure with preserved ejection fraction (HFpEF). Recent studies have suggested a role for coronary microvascular dysfunction in HFpEF. Rb-82 cardiac positron emission tomography imaging is a noninvasive, quantitative approach to measuring myocardial flow reserve (MFR), a surrogate marker for coronary vascular health. The aim of this study was to determine whether abnormalities exist in MFR in patients with HFpEF without epicardial coronary artery disease. METHODS AND RESULTS: A total of 376 patients with ejection fraction ≥50%, no known history of obstructive coronary artery disease, and a confirmed diagnosis of heart failure (n=78) were compared with patients with no evidence of heart failure (n=298), further stratified into those with (n=186) and without (n=112) hypertension. Global and regional left ventricular MFR was calculated as stress/rest myocardial blood flow using Rb-82 positron emission tomography. Patients with HFpEF were more likely to be older, female, and have comorbid hypertension, diabetes mellitus, dyslipidemia, atrial fibrillation, anemia, and renal dysfunction. HFpEF was associated with a significant reduction in global MFR (2.16±0.69 in HFpEF versus 2.54±0.80 in hypertensive controls; P<0.02 and 2.89±0.70 in normotensive controls; P<0.001). A diagnosis of HFpEF was associated with 2.62 times greater unadjusted odds of having low global MFR (defined as <2.0) and remained a significant predictor of reduced global MFR after adjusting for comorbidities. CONCLUSIONS: HFpEF, in the absence of known history for obstructive epicardial coronary artery disease, is associated with reduced MFR independent of other risk factors.
[Mh] Termos MeSH primário: Circulação Coronária
Insuficiência Cardíaca/fisiopatologia
Microcirculação
Volume Sistólico
Função Ventricular Esquerda
[Mh] Termos MeSH secundário: Fatores Etários
Idoso
Distribuição de Qui-Quadrado
Comorbidade
Estudos Transversais
Bases de Dados Factuais
Ecocardiografia Doppler
Feminino
Insuficiência Cardíaca/diagnóstico por imagem
Seres Humanos
Modelos Lineares
Masculino
Meia-Idade
Análise Multivariada
Imagem de Perfusão do Miocárdio/métodos
Razão de Chances
Tomografia por Emissão de Pósitrons
Valor Preditivo dos Testes
Compostos Radiofarmacêuticos/administração & dosagem
Estudos Retrospectivos
Fatores de Risco
Rubídio/administração & dosagem
Fatores Sexuais
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Radiopharmaceuticals); MLT4718TJW (Rubidium)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170703
[Lr] Data última revisão:
170703
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160715
[St] Status:MEDLINE



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