Base de dados : MEDLINE
Pesquisa : D01.268.556.607 [Categoria DeCS]
Referências encontradas : 20357 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 2036 ir para página                         

  1 / 20357 MEDLINE  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28470986
[Au] Autor:Wang L; Wang Y; Chai Y; Kang Y; Sun C; Zeng S
[Ad] Endereço:Institute of Drug Metabolism and Pharmaceutical Analysis, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, 310058, China.
[Ti] Título:Nickel(II)-assisted enantiomeric differentiation and quantitation of tadalafil by direct electrospray ionization mass spectrometry.
[So] Source:J Mass Spectrom;52(7):411-416, 2017 Jul.
[Is] ISSN:1096-9888
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A facile method based on electrospray mass spectrometry was established and validated for the differentiation of enantiomeric tadalafil isomers without using chiral chromatographic separation. The enantiomers were coupled with a chiral selector to form diastereomeric complex ions. Nickel-tadalafil complexes, [Ni (tadalafil)(l-Trp)-H] , produced a characteristic fragment ion at m/z 524 by loss of 1-methyl-1,6-dihydropyrazine-2,5-dione via collision-induced dissociation. The relative abundance of this fragment ion to the precursor contributed to differentiate tadalafil enantiomers, and energy-resolved product-ion spectra were applied to determine the molar composition of tadalafil in the mixture (R,R and S,S) as well. In addition, the other two forms of stereomeric isomers of tadalafil (R,S and S,R) could be also distinguished and analyzed by this method. The method was validated in different types of mass spectrometers (AB quadrupole time-of-flight and Bruker ion trap) and also verified by a chiral high-performance liquid chromatography coupled with quadrupole time-of-flight. The chiral determination of tadalafil using MS method proved to be rapid (1-min run time for each sample) and to have the same accuracy and precision comparable to chiral liquid chromatography mass spectrometry methods. This method provides an alternative to commonly used chromatographic technique for chiral determination and is particularly useful in rapid screening in enantioselective synthesis and enantiomeric impurity detection in pharmaceutical industry. Copyright © 2017 John Wiley & Sons, Ltd.
[Mh] Termos MeSH primário: Complexos de Coordenação/análise
Níquel/química
Inibidores da Fosfodiesterase 5/análise
Tadalafila/análise
[Mh] Termos MeSH secundário: Cromatografia Líquida de Alta Pressão/métodos
Complexos de Coordenação/química
Contaminação de Medicamentos
Conformação Molecular
Inibidores da Fosfodiesterase 5/química
Espectrometria de Massas por Ionização por Electrospray/métodos
Estereoisomerismo
Tadalafila/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; VALIDATION STUDIES
[Nm] Nome de substância:
0 (Coordination Complexes); 0 (Phosphodiesterase 5 Inhibitors); 742SXX0ICT (Tadalafil); 7OV03QG267 (Nickel)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170505
[St] Status:MEDLINE
[do] DOI:10.1002/jms.3939


  2 / 20357 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29181620
[Au] Autor:Real MIH; Azam HM; Majed N
[Ad] Endereço:Department of Civil Engineering, University of Asia Pacific, Dhaka, Bangladesh.
[Ti] Título:Consumption of heavy metal contaminated foods and associated risks in Bangladesh.
[So] Source:Environ Monit Assess;189(12):651, 2017 Nov 27.
[Is] ISSN:1573-2959
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:This study investigated the magnitude of heavy metal contamination and determined the carcinogenic as well as non-carcinogenic risks associated with selected food consumption in Bangladesh. Commonly consumed varieties of rice, vegetables, and fish samples were analyzed to measure the concentrations of heavy metals such as cadmium, chromium, lead, arsenic, manganese, nickel, and zinc. These staple food items showed the greatest probabilities of heavy metal contamination in different phases of their production and marketing. Wide variations of metal concentrations were observed. Specifically, estimated daily intakes of arsenic and cadmium exceeded allowable daily intakes in all three food items. Toxicity scores of the metals were evaluated, and a comprehensive risk assessment was conducted to quantify the risks associated with the daily food consumption. Except for cadmium and lead in vegetables, all the contaminants present in each food item posed significant levels of carcinogenic risks up to 2.99 × 10 compared to the EPA recommended carcinogenic risk level of 1.0 × 10 . Cadmium and arsenic intake due to rice consumption also posed unsafe levels of non-carcinogenic risks of 4.587 and 6.648, respectively, compared to the EPA recommended non-carcinogenic risk level of 1.0. Finally, a revised set of permissible limits was proposed for the heavy metals detected in the food items. Those permissible limits would ensure the risks associated with food consumption below the allowable carcinogenic and non-carcinogenic risk levels. Thus, this comprehensive approach would provide guidelines to formulate adequate control measures and regulatory limits of toxic metals in foods produced and marketed in Bangladesh.
[Mh] Termos MeSH primário: Exposição Dietética/estatística & dados numéricos
Poluentes Ambientais/análise
Contaminação de Alimentos/análise
Metais Pesados/análise
[Mh] Termos MeSH secundário: Animais
Arsênico/análise
Bangladesh
Cádmio/análise
Cromo
Monitoramento Ambiental
Peixes
Contaminação de Alimentos/estatística & dados numéricos
Seres Humanos
Níquel
Medição de Risco
Verduras/química
Zinco
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Environmental Pollutants); 0 (Metals, Heavy); 00BH33GNGH (Cadmium); 0R0008Q3JB (Chromium); 7OV03QG267 (Nickel); J41CSQ7QDS (Zinc); N712M78A8G (Arsenic)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180307
[Lr] Data última revisão:
180307
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171129
[St] Status:MEDLINE
[do] DOI:10.1007/s10661-017-6362-z


  3 / 20357 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
[PMID]:29424999
[Au] Autor:Tusupkaliev BT; Bermagambetova SK; Tusupkaliev AB
[Ti] Título:[Morbidity and content of microelements in the blood of school children residing near chromium enterprises].
[So] Source:Gig Sanit;95(7):655-8, 2016.
[Is] ISSN:0016-9900
[Cp] País de publicação:Russia (Federation)
[La] Idioma:rus
[Ab] Resumo:For the first time there was investigated the content of certain microelements in the blood of children of school age residing in the city of Aktobe (Kazakhstan), where the global giants, mining andprocessing chrome ore productions are located. There were determined blood levels of chromium, nickel, lead in children. There was shown the difference in the blood content of microelements in children, depending on region of residence and their correlation with the core indices of health and disease.
[Mh] Termos MeSH primário: Indústria Química
Cromo/sangue
Exposição Ambiental
Doença Ambiental
Chumbo/sangue
Mineração
Níquel/sangue
[Mh] Termos MeSH secundário: Criança
Exposição Ambiental/efeitos adversos
Exposição Ambiental/análise
Exposição Ambiental/estatística & dados numéricos
Doença Ambiental/sangue
Doença Ambiental/classificação
Doença Ambiental/epidemiologia
Feminino
Disparidades nos Níveis de Saúde
Seres Humanos
Cazaquistão/epidemiologia
Masculino
Medição de Risco
Estatística como Assunto
Oligoelementos/sangue
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Trace Elements); 0R0008Q3JB (Chromium); 2P299V784P (Lead); 7OV03QG267 (Nickel)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180301
[Lr] Data última revisão:
180301
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180210
[St] Status:MEDLINE


  4 / 20357 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
[PMID]:29424205
[Au] Autor:Siurin SV; Shilov VV
[Ti] Título:[Features of health disorders in miners employed at northern copper-nickel mines].
[So] Source:Gig Sanit;95(5):455-9, 2016.
[Is] ISSN:0016-9900
[Cp] País de publicação:Russia (Federation)
[La] Idioma:rus
[Ab] Resumo:The aim of the study was to assess the influence of different working conditions on the health of 1523 copper-nickel miners of the Kola High North. The low degree of mechanization of mining operations was established to be related to more higher levels of vibration, noise and physical overloads. The working in such conditions, when compared with high mining mechanization, leads to a decrease in the number of conditionally healthy workers (12% and 20.7%, p <0.001) an increase in the number of diseases in the one worker (2,94 ± 0,10 and 2.13 ± 0.07 cases, p <0,001), the increase of the risk of occupational diseases (OD) of the musculoskeletal (RR = 2.31) and nervous (RR = 2.07) systems, vibration disease (RR = 1.70), neuro-sensory hearing loss (RR = 1.90). Both at low and high degree of mechanization of mining operations the greatest risk of occupational diseases in noted in drifters (OR = 5.68), at that it was higher at hand mining than at mechanized mining (RR = 1.44). There was made a conclusion about the need to improve the complex of measures for the preservation of health in this group of workers, especially engaged in the performance of tunnel works.
[Mh] Termos MeSH primário: Cobre
Mineração
Níquel
Doenças Profissionais
[Mh] Termos MeSH secundário: Adulto
Regiões Árticas/epidemiologia
Seres Humanos
Masculino
Mineração/normas
Mineração/estatística & dados numéricos
Determinação de Necessidades de Cuidados de Saúde
Doenças Profissionais/induzido quimicamente
Doenças Profissionais/diagnóstico
Doenças Profissionais/epidemiologia
Doenças Profissionais/prevenção & controle
Saúde do Trabalhador/estatística & dados numéricos
Serviços de Saúde do Trabalhador/normas
Saúde Pública/métodos
Medição de Risco
Fatores de Risco
Federação Russa/epidemiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
789U1901C5 (Copper); 7OV03QG267 (Nickel)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180301
[Lr] Data última revisão:
180301
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180210
[St] Status:MEDLINE


  5 / 20357 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29390311
[Au] Autor:Suwarsa O; Rahardjo RM; Sutedja E; Dharmadji HP; Hindritiani R; Gunawan H
[Ad] Endereço:Division of Allergy and Immunology, Department of Dermatology and Venereology, Faculty of Medicine, Universitas Padjadjaran-Dr. Hasan Sadikin Hospital, Bandung, Indonesia.
[Ti] Título:Systemic contact dermatitis due to corrosion of titanium-coated nickel and cobalt bone plate fixation: A case report.
[So] Source:Medicine (Baltimore);96(50):e9120, 2017 Dec.
[Is] ISSN:1536-5964
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:RATIONALE: Corrosion refers to the degradation of a material that occurs following its interaction with other substances in the environment. Corrosion of metallic substances into tissues may lead to inflammatory responses such as systemic contact dermatitis (SCD), a skin condition where an individual who has previously been sensitized to a particular allergen via the cutaneous route will subsequently react to same allergen via the systemic route. This condition occurs following exposure to allergens such as metals, medications, and certain food substances. In recent years, the use of metal plates for internal fixation has become increasingly common in bone fracture repairs. PATIENT CONCERNS: A 34-year-old Indonesian male presented with systemic erythema with itching 7 days following a bone plate fixation as a management for mandibular fracture. DIAGNOSES: Physical examination showed pruritic red macules, papules, and scales on almost his entire body, along with facial swelling. The patch test results revealed a positive reaction to nickel and cobalt. Therefore, the patient was diagnosed with SCD. INTERVENTIONS: The patient was treated with topical and systemic corticosteroids as well as bone plate removal. OUTCOME: After treatment, the eruption turned brown, the itching was resolved, and there were no facial swelling as well. LESSONS: This case report highlights the need to consider the occurrence of SCD in patients following bone plate fixation.
[Mh] Termos MeSH primário: Placas Ósseas
Dermatite de Contato/etiologia
Dermatite de Contato/terapia
Fixação Interna de Fraturas/instrumentação
Fraturas Mandibulares/cirurgia
[Mh] Termos MeSH secundário: Corticosteroides/uso terapêutico
Adulto
Cobalto
Corrosão
Remoção de Dispositivo
Seres Humanos
Masculino
Níquel
Testes do Emplastro
Titânio
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Adrenal Cortex Hormones); 3G0H8C9362 (Cobalt); 7OV03QG267 (Nickel); D1JT611TNE (Titanium)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180301
[Lr] Data última revisão:
180301
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:180203
[St] Status:MEDLINE
[do] DOI:10.1097/MD.0000000000009120


  6 / 20357 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29193978
[Au] Autor:Balestri D; Roux Y; Mattarozzi M; Mucchino C; Heux L; Brazzolotto D; Artero V; Duboc C; Pelagatti P; Marchiò L; Gennari M
[Ad] Endereço:Dipartimento di Scienze Chimiche, della Vita e della Sostenibilità Ambientale, Università degli Studi di Parma , Parco Area delle Scienze 17A, 43124 Parma, Italy.
[Ti] Título:Heterogenization of a [NiFe] Hydrogenase Mimic through Simple and Efficient Encapsulation into a Mesoporous MOF.
[So] Source:Inorg Chem;56(24):14801-14808, 2017 Dec 18.
[Is] ISSN:1520-510X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In the quest for new, efficient, and noble-metal-free H -evolution catalysts, hydrogenase enzymes are a source of inspiration. Here, we describe the development of a new hybrid material based on a structural and functional [NiFe]-hydrogenase model complex (NiFe) incorporated into the Zr-based MOF PCN-777. The bulk NiFe@PCN-777 material was synthesized by simple encapsulation. Characterization by solid-state NMR and IR spectroscopy, SEM-EDX, ICP-OES, and gas adsorption confirmed the inclusion of the guest. FTO-supported thin films of the NiFe@PCN-777 composite were obtained by electrophoretic deposition of the bulk material and characterized by SEM-EDX, ICP-OES, and cyclic voltammetry. The average surface concentration of electroactive NiFe catalyst in the film was found to be ∼9.6 × 10 mol cm , implying that a surprisingly high fraction (37%) of NiFe units incorporated in the MOF are electroactive. By cyclic voltammetry, we showed that NiFe maintains its electrocatalytic capabilities for H reduction inside the MOF cavities, even if under controlled-potential electrolysis conditions the activity of NiFe cannot be discerned from that of free PCN-777 and FTO.
[Mh] Termos MeSH primário: Materiais Biomiméticos/química
Hidrogenase/química
Ferro/química
Estruturas Metalorgânicas/química
Níquel/química
Zircônio/química
[Mh] Termos MeSH secundário: Catálise
Técnicas Eletroquímicas
Modelos Moleculares
Oxirredução
Prótons
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Metal-Organic Frameworks); 0 (Protons); 7OV03QG267 (Nickel); C6V6S92N3C (Zirconium); E1UOL152H7 (Iron); EC 1.12.- (nickel-iron hydrogenase); EC 1.12.7.2 (Hydrogenase)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180228
[Lr] Data última revisão:
180228
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171202
[St] Status:MEDLINE
[do] DOI:10.1021/acs.inorgchem.7b01824


  7 / 20357 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
[PMID]:29185319
[Au] Autor:Brave D; Trattner B
[Ti] Título:NiTi Rotary Instrumentation: Achieving Its Full Potential.
[So] Source:Dent Today;35(10):86, 88, 90, 92, 2016 Oct.
[Is] ISSN:8750-2186
[Cp] País de publicação:United States
[La] Idioma:eng
[Mh] Termos MeSH primário: Instrumentos Odontológicos
Níquel
Titânio
[Mh] Termos MeSH secundário: Desenho de Equipamento
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
12035-60-8 (titanium nickelide); 7OV03QG267 (Nickel); D1JT611TNE (Titanium)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180220
[Lr] Data última revisão:
180220
[Sb] Subgrupo de revista:D
[Da] Data de entrada para processamento:171201
[St] Status:MEDLINE


  8 / 20357 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28992475
[Au] Autor:Akhtar MJ; Ullah S; Ahmad I; Rauf A; Nadeem SM; Khan MY; Hussain S; Bulgariu L
[Ad] Endereço:Institute of Soil & Environmental Sciences, University of Agriculture Faisalabad, 38040, Pakistan.
[Ti] Título:Nickel phytoextraction through bacterial inoculation in Raphanus sativus.
[So] Source:Chemosphere;190:234-242, 2018 Jan.
[Is] ISSN:1879-1298
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A pot experiment was conducted to evaluate the potential of two plant growth promoting rhizobacteria (PGPR) viz. Bacillus sp. CIK-516 and Stenotrophomonas sp. CIK-517Y for improving the growth and Ni uptake of radish (Raphanus sativus) in the presence of four different levels of Ni contamination (0, 50, 100, 150 mg Ni kg soil). Plant growth, dry biomass, chlorophyll and nitrogen contents were significantly reduced by the exogenous application of Ni, however, bacterial inoculation diluted the negative impacts of Ni stress on radish by improving these parameters. PGPR strain CIK-516 increased root length (9-27%), shoot length (8-27%), root dry biomass (2-32%), shoot dry biomass (9-51%), root girth (6-48%), total chlorophyll (4-38%) and shoot nitrogen contents (11-15%) in Ni contaminated and non-contaminated soils. Positive regulation of chlorophyll and nitrogen contents by the inoculated plants shows plant tolerance mechanism of Ni stress. Bacterial strain (CIK-516) exhibited indole acetic acid and 1-amino-cyclopropane-1-carboxylate deaminase potentials which would have helped radish plant to stabilize in Ni contaminated soil and thereby increased Ni uptake (24-257 in shoot and 58-609 in root mg kg dry biomass) and facilitated accumulation in radish (bioaccumulation factor = 0.6-1.7) depending upon soil Ni contamination. Based on the findings of this study, it might be suggested that inoculation with bacterial strain CIK-516 could be an efficient tool for enhanced Ni phytoextraction in radish.
[Mh] Termos MeSH primário: Recuperação e Remediação Ambiental/métodos
Níquel/isolamento & purificação
Raphanus/microbiologia
Poluentes do Solo/isolamento & purificação
[Mh] Termos MeSH secundário: Inoculantes Agrícolas
Bacillus/metabolismo
Clorofila/análise
Níquel/farmacologia
Nitrogênio/análise
Desenvolvimento Vegetal/efeitos dos fármacos
Raízes de Plantas/crescimento & desenvolvimento
Raphanus/crescimento & desenvolvimento
Rhizobiaceae/metabolismo
Poluentes do Solo/farmacocinética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Soil Pollutants); 1406-65-1 (Chlorophyll); 7OV03QG267 (Nickel); N762921K75 (Nitrogen)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180215
[Lr] Data última revisão:
180215
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171010
[St] Status:MEDLINE


  9 / 20357 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29325850
[Au] Autor:Nejat Dehkordi M; Akerman B
[Ad] Endereço:Department of Basic Science, Islamic Azad University, Shahrekord Branch, Shahrekord, Iran; Department of Chemical and Biological Engineering, Chalmers University of Technology, SE-412 96, Gothenburg, Sweden. Electronic address: m.nejat@iaushk.ac.ir.
[Ti] Título:Interaction of DNA with water soluble complex of Nickle and formation of DNA cross-links.
[So] Source:Chem Biol Interact;282:55-62, 2018 Feb 25.
[Is] ISSN:1872-7786
[Cp] País de publicação:Ireland
[La] Idioma:eng
[Ab] Resumo:Interaction of double stranded DNA with bulky and hydrophobic Salen type Schiff base complex: [N, N' Bis [3- tert-butyl-5-[triphenyl-phosphonium - methyl] - salicylidene] 1,2 ethylene-diamine nickel(III) acetate (refer to Ni Salen complex) was extensively investigated using the spectroscopic techniques and gel electrophoresis. Absorption titration experiment showed the hypochromic effect and the significant red shift of the complex absorption. In competition experiments with ethidium bromide (EB), Ni Salen complex exhibited non-competitive binding at high concentrations. UV-vis absorption and fluorescence emission data agreed on a binding constant of (1.64 ±â€¯0.01) µM , thereby showing the strong interaction of the complex with DNA; also, a binding site size of 2.33 ±â€¯0.01 base pairs per complex was achieved. Thermal denaturation experiment showed that T of calf thymus-DNA was increased by approximately 10 °C at a molar ratio of the dye/base of 0.2. The CD spectra of DNA exhibited an increase in both positive and negative peaks without any shift in the position of bands upon addition of the complex. The amplitude of the LD spectra of DNA was decreased in the presence of the complex. Reduced linear dichroism (LD ) revealed that the transition moment of complex was parallel to the DNA helix axis. Gel electrophoresis experiments confirmed that Ni Salen complex had no nuclease/DNA cleaving activity; also, DNA-DNA cross links were formed at high concentrations of complex, leading to the aggregation of DNA.
[Mh] Termos MeSH primário: DNA/química
Níquel/química
RNA de Cadeia Dupla/química
Água/química
[Mh] Termos MeSH secundário: Animais
Sítios de Ligação
Bovinos
Eletroforese em Gel de Ágar/métodos
Etídio/química
Etilenodiaminas/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Ethylenediamines); 0 (RNA, Double-Stranded); 059QF0KO0R (Water); 7OV03QG267 (Nickel); 9007-49-2 (DNA); 91080-16-9 (calf thymus DNA); 94-93-9 (disalicylaldehyde ethylenediamine); EN464416SI (Ethidium)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180209
[Lr] Data última revisão:
180209
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180113
[St] Status:MEDLINE


  10 / 20357 MEDLINE  
              first record previous record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28467834
[Au] Autor:Yin R; Mo J; Dai J; Wang H
[Ad] Endereço:State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences , Beijing 100085, China.
[Ti] Título:Nickel(II) Inhibits Tet-Mediated 5-Methylcytosine Oxidation by High Affinity Displacement of the Cofactor Iron(II).
[So] Source:ACS Chem Biol;12(6):1494-1498, 2017 06 16.
[Is] ISSN:1554-8937
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Ten-eleven translocation (Tet) family proteins are Fe(II)- and 2-oxoglutarate-dependent dioxygenases that regulate the dynamics of DNA methylation by catalyzing the oxidation of DNA 5-methylcytosine (5mC). To exert physiologically important functions, redox-active iron chelated in the catalytic center of Tet proteins directly involves the oxidation of the multiple substrates. To understand the function and interaction network of Tet dioxygenases, it is interesting to obtain high affinity and a specific inhibitor. Surprisingly, here we found that natural Ni(II) ion can bind to the Fe(II)-chelating motif (HXD) with an affinity of 7.5-fold as high as Fe(II). Consistently, we further found that Ni(II) ion can displace the cofactor Fe(II) of Tet dioxygenases and inhibit Tet-mediated 5mC oxidation activity with an estimated IC of 1.2 µM. Essentially, Ni(II) can be used as a high affinity and selective inhibitor to explore the function and dynamics of Tet proteins.
[Mh] Termos MeSH primário: 5-Metilcitosina/metabolismo
Dioxigenases/antagonistas & inibidores
Ferro/metabolismo
Níquel/farmacologia
[Mh] Termos MeSH secundário: Coenzimas
Compostos Ferrosos
Seres Humanos
Concentração Inibidora 50
Oxirredução/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Coenzymes); 0 (Ferrous Compounds); 6R795CQT4H (5-Methylcytosine); 7OV03QG267 (Nickel); E1UOL152H7 (Iron); EC 1.13.11.- (Dioxygenases)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:180201
[Lr] Data última revisão:
180201
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170504
[St] Status:MEDLINE
[do] DOI:10.1021/acschembio.7b00261



página 1 de 2036 ir para página                         
   


Refinar a pesquisa
  Base de dados : MEDLINE Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde