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[PMID]:29446584
[Au] Autor:Sosedova LM; Novikov MA; Titov EA; Rukavishnikov VS
[Ti] Título:[Induction of apoptosis in neurons of white rats under exposure of nanobiocomposite based on ag (0) nanoparticles and arabinogalactan].
[So] Source:Gig Sanit;95(12):1210-13, 2016.
[Is] ISSN:0016-9900
[Cp] País de publicação:Russia (Federation)
[La] Idioma:rus
[Ab] Resumo:There are presented results of the immunohistochemical study of neural tissue of outbred albino rats exposed for 9 days to the influence of the silver nanobiocomposite consisted of silver nanoparticles encapsulated into a matrix of a natural polymer - arabinogalactan. The research of albino rats was performed in 2 stages: half of the rats in each groups were decapitated immediately after the exposure (early period) and the rest animals - 6 months after the end of exposure (remote period). The impact of the studied substance was proved to cause functional changes in cells of the nervous tissue. After the subacute administration of the nanobiocomposite - argentum-arabinogalactan (nano-Ag-AG) in cells of the nervous tissue of the brain of albino rats the expression of apoptotic and anti-apoptotic protein (caspase-3 and bcl-2) was established to be changed. The number of normal neurons producing protein caspase-3 sharply increases. Herewith the number of immunonegative neurons fairly declines. Along with this there is noted the high level of bcl-2 content, one function ofwhich is the preclusion ofapoptosis. In preparations there is revealed a significant gain in the number of bcl-2 expressing neurons, however, the protective effect of the protein is not fully realized, that leads to the significantly increase in the content of damaged hyperchromatic cells. The evaluation of results of the immunohistochemical study of the nervous tissue of albino rats according to data concerning the proteins caspase-3 and bcl-2 expression permits to make a conclusion about the capability of nanoargentum encapsulated into polymer matrix by passing the blood-brain barrier to induce the triggering apoptosis cascade in neurons of the cerebral cortex.
[Mh] Termos MeSH primário: Encéfalo
Galactanos/farmacologia
Larix
Nanopartículas Metálicas/efeitos adversos
Prata
[Mh] Termos MeSH secundário: Animais
Apoptose/efeitos dos fármacos
Biopolímeros/farmacologia
Barreira Hematoencefálica/metabolismo
Encéfalo/efeitos dos fármacos
Encéfalo/patologia
Encéfalo/fisiopatologia
Caspase 3/metabolismo
Imuno-Histoquímica
Modelos Animais
Preparações de Plantas/farmacologia
Proteínas Proto-Oncogênicas c-bcl-2/metabolismo
Ratos
Prata/efeitos adversos
Prata/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biopolymers); 0 (Galactans); 0 (Plant Preparations); 0 (Proto-Oncogene Proteins c-bcl-2); 3M4G523W1G (Silver); EC 3.4.22.- (Caspase 3); SL4SX1O487 (arabinogalactan)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180216
[St] Status:MEDLINE


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[PMID]:29238194
[Au] Autor:Du C; Yan H; Liang J; Luo A; Wang L; Zhu J; Xiong H; Chen Y
[Ad] Endereço:Hubei Province Key Laboratory of Biotechnology of Chinese Traditional Medicine, Hubei Collaborative Innovation Center for Green Transformation of Bio-resources, Hubei University, Wuhan.
[Ti] Título:Polyethyleneimine-capped silver nanoclusters for microRNA oligonucleotide delivery and bacterial inhibition.
[So] Source:Int J Nanomedicine;12:8599-8613, 2017.
[Is] ISSN:1178-2013
[Cp] País de publicação:New Zealand
[La] Idioma:eng
[Ab] Resumo:Efficient and safe nonviral gene delivery systems are a prerequisite for the clinical application of therapeutic genes. In this paper, polyethyleneimine-capped silver nanoclusters (PEI-AgNCs) were prepared for the purpose of microRNA (miRNA) delivery. The resultant PEI-AgNCs were characterized by a photoluminescence assay and transmission electron microscopy. A cytotoxicity assay showed that PEI-AgNCs exhibit relatively low cytotoxicity. Interestingly, PEI-AgNCs were confirmed to transfect miRNA mimics more effectively than PEI in HepG2 and 293A cells. In this regard, hsa-miR-21 or hsa-miR-221 mimics (miR-21/221m) were transported into HepG2 cells by using PEI-AgNCs. The miR-21/221 expression was determined post-transfection by quantitative real-time polymerase chain reaction. Compared with the negative control, PEI-AgNCs/miR-21/221m groups exhibited higher miR-21/221 levels. In addition, AgNCs endow PEI with stronger antibacterial activity, and this advantage provided PEI-AgNCs the potential to prevent bacterial contamination during the transfection process. Furthermore, we showed that PEI-AgNCs are viable nanomaterials for plain imaging of the cells by laser scanning confocal microscopy, indicating great potential as an ideal fluorescent probe to track the transfection behavior. These results demonstrated that PEI-AgNCs are promising and novel nonviral vectors for gene delivery.
[Mh] Termos MeSH primário: MicroRNAs/administração & dosagem
Nanoestruturas/química
Polietilenoimina/química
Prata/administração & dosagem
Transfecção/métodos
[Mh] Termos MeSH secundário: Antibacterianos/administração & dosagem
Antibacterianos/farmacologia
Escherichia coli/efeitos dos fármacos
Células HEK293
Células Hep G2
Seres Humanos
MicroRNAs/genética
Microscopia Confocal
Nanoestruturas/administração & dosagem
Oligonucleotídeos/administração & dosagem
Polietilenoimina/farmacologia
Reação em Cadeia da Polimerase em Tempo Real
Prata/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (MIRN21 microRNA, human); 0 (MIRN221 microRNA, human); 0 (MicroRNAs); 0 (Oligonucleotides); 3M4G523W1G (Silver); 9002-98-6 (Polyethyleneimine)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171215
[St] Status:MEDLINE
[do] DOI:10.2147/IJN.S146968


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[PMID]:28942273
[Au] Autor:Barabanov PV; Gerasimov AV; Blinov AV; Kravtsov AA; Kravtsov VA
[Ad] Endereço:North-Caucasus Federal University, Russian Federation.
[Ti] Título:Influence of nanosilver on the efficiency of Pisum sativum crops germination.
[So] Source:Ecotoxicol Environ Saf;147:715-719, 2018 Jan.
[Is] ISSN:1090-2414
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The study is aimed to investigate the impact of silver nanoparticles on germination of Pisum sativum pea seeds. The influence of synthesized silver nanoparticles on root length and percentage of germinated seeds was revealed. It was found that nanosilver treatment agents do not affect the germination of pea seeds negatively at low concentrations. Also, the treatment of pea seeds with silver nanoparticles provide a significant positive effect on the root length of pea seeds.
[Mh] Termos MeSH primário: Germinação/efeitos dos fármacos
Nanopartículas Metálicas/química
Ervilhas/efeitos dos fármacos
Sementes/efeitos dos fármacos
Prata/farmacologia
[Mh] Termos MeSH secundário: Relação Dose-Resposta a Droga
Tamanho da Partícula
Ervilhas/crescimento & desenvolvimento
Sementes/crescimento & desenvolvimento
Prata/química
Propriedades de Superfície
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
3M4G523W1G (Silver)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170925
[St] Status:MEDLINE


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[PMID]:29215338
[Au] Autor:Kart D; Kustimur AS; Sagiroglu M; Kalkanci A
[Ad] Endereço:Department of Pharmaceutical Microbiology, Hacettepe University Faculty of Pharmacy, Ankara, Turkey.
[Ti] Título:Evaluation of Antimicrobial Durability and Anti-Biofilm Effects in Urinary Catheters Against Clinical Isolates and Reference Strains.
[So] Source:Balkan Med J;34(6):546-552, 2017 12 01.
[Is] ISSN:2146-3131
[Cp] País de publicação:Turkey
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: and biofilms are major causes of catheter-associated urinary tract infections. Antimicrobial-coated or impregnated urinary catheters are seen as a possible way to prevent these infections. AIMS: To determine the biofilm-forming ability of 89 isolates from urinary tract infections and to compare several urinary catheters for antimicrobial durability and the inhibitory effects on biofilm formation of different laboratory strains and clinical isolates of . STUDY DESIGN: experimental study. METHODS: The biofilm forming ability of isolates was determined by the crystal violet staining and plate counting methods. For comparison of urinary catheters, biofilms of 45 isolates from the catheter samples of hospitalized patients and five laboratory strains of ATCC25922, ATCC35984, ATCC27853, ATCC29212 and ATCC90028 were formed on the catheters in 24-well tissue culture plates. Scanning electron microscopy analysis was performed to observe biofilms. RESULTS: All 89 isolates were found to be biofilm positive. Nitrofurazone-impregnated catheters significantly reduced the cell counts of isolates and completely inhibited the formation of and biofilms compared with the others. Regarding reduction of biofilm cell counts, a hydrophilic-coated catheter was more effective against , whereas a silver-coated catheter was found to be more effective against . The nitrofurazone-impregnated catheter had the best antimicrobial durability. CONCLUSION: Urine isolates of had considerable ability with respect to biofilm formation. The nitrofurazone-impregnated catheter was the most effective against all tested bacteria; however, the effect of a hydrophilic or silver-coated catheter depends on the species present in it.
[Mh] Termos MeSH primário: Anti-Infecciosos/farmacologia
Biofilmes/efeitos dos fármacos
Infecções Relacionadas a Cateter/microbiologia
Cateteres de Demora/microbiologia
Enterococcus faecalis/efeitos dos fármacos
Nitrofurazona/farmacologia
Infecções Urinárias/microbiologia
[Mh] Termos MeSH secundário: Infecções Relacionadas a Cateter/prevenção & controle
Materiais Revestidos Biocompatíveis
Enterococcus faecalis/isolamento & purificação
Seres Humanos
Técnicas In Vitro
Testes de Sensibilidade Microbiana
Silicones
Prata
Cateterismo Urinário/efeitos adversos
Infecções Urinárias/prevenção & controle
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Anti-Infective Agents); 0 (Coated Materials, Biocompatible); 0 (Silicones); 3M4G523W1G (Silver); X8XI70B5Z6 (Nitrofurazone)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180308
[Lr] Data última revisão:
180308
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171208
[St] Status:MEDLINE
[do] DOI:10.4274/balkanmedj.2016.1853


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[PMID]:28822261
[Au] Autor:Rasheed W; Shah MR; Perveen S; Ahmed S; Uzzaman S
[Ad] Endereço:H.E.J. Research Institute of Chemistry, International Centre for Chemical and Biological Sciences, University of Karachi, Karachi 75270, Pakistan.
[Ti] Título:Revelation of susceptibility differences due to Hg(II) accumulation in Streptococcus pyogenes against CX-AgNPs and Cefixime by atomic force microscopy.
[So] Source:Ecotoxicol Environ Saf;147:9-16, 2018 Jan.
[Is] ISSN:1090-2414
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Solution based method for the formation of chemically modified silver nanoparticles (CX-AgNPs) using Cefixime as stabilizing and reducing agent was developed. The CX-AgNPs were characterized by AFM, UV-visible, FT-IR and MALDI-TOF MS. Bactericidal efficiency of CX-AgNPs and Cefixime against Streptococcus pyogenes was evaluated. Afterwards, susceptibility differences of Streptococcus pyogenes due to accumulation of Hg(II) against CX-AgNPs and Cefixime were estimated and validated through Atomic force microscopy. Selectivity and sensitivity of CX-AgNPs against Hg(II) was evaluated in a systematic manner. The CX-AgNPs was titrated against optically silent Hg(II) which induced enhancement in the SPR band of CX-AgNPs. The increase in intensity of SPR band of CX-AgNPs was determined to be proportionate to the concentration of Hg(II) in the range of 33.3-700µM obeying linear regression equation of y = 0.125x + 8.962 with the detection limit of 0.10µM and the coefficient of determination equals to 0.985 (n = 3). The association constant Ka of CX-AgNPs-Hg(II) was found to be 386.0095mol dm by using the Benesi Hildebrand plot.
[Mh] Termos MeSH primário: Antibacterianos/farmacologia
Cefixima/farmacologia
Mercúrio/metabolismo
Nanopartículas Metálicas/química
Nanoconjugados/química
Prata/farmacologia
Streptococcus pyogenes/efeitos dos fármacos
[Mh] Termos MeSH secundário: Antibacterianos/química
Cefixima/química
Limite de Detecção
Testes de Sensibilidade Microbiana
Microscopia de Força Atômica
Prata/química
Espectroscopia de Infravermelho com Transformada de Fourier
Streptococcus pyogenes/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Nanoconjugates); 3M4G523W1G (Silver); 97I1C92E55 (Cefixime); FXS1BY2PGL (Mercury)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180308
[Lr] Data última revisão:
180308
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170820
[St] Status:MEDLINE


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[PMID]:29412224
[Au] Autor:El-Batal AI; Ahmed SF
[Ad] Endereço:National Centre for Radiation Research and Technology - NCRRT, Atomic Energy Authority, Drug Radiation Research Department, Nasr City, Cairo, Egypt.
[Ti] Título:Therapeutic effect of Aloe vera and silver nanoparticles on acid-induced oral ulcer in gamma-irradiated mice.
[So] Source:Braz Oral Res;32:e004, 2018 Feb 05.
[Is] ISSN:1807-3107
[Cp] País de publicação:Brazil
[La] Idioma:eng
[Ab] Resumo:Radiation combined injury, a life-threatening condition, has higher mortality than simple radiation injury. The aim of the present study was to analyze the efficiency of Aloe vera and silver nanoparticles in improving the healing of ulcerated oral mucosa after irradiation. Thirty male Albino mice were divided into five groups: control, radiation, Aloe vera (AV), silver nanoparticles (NS), and AV+NS. The mice were exposed to whole body 6Gy gamma-radiation. After one hour, 20% acetic acid was injected into the submucosal layer of the lower lip for ulcer induction. The animals received topical treatment with the assigned substances for 5 days. Lip specimens were subjected to hematoxylin and eosin and anti alpha-smooth muscle actin immunohistochemical staining. Results demonstrated occurance of ulcer three days post irradiation in all groups except in the AV+NS group where only epithelial detachment was developed. After seven days, data revealed persistent ulcer in radiation group, and almost normal epithelium in the AV+NS group. A significant reduction of epithelial thickness was detected in all groups at the third day as compared to control. At the seventh day, only the AV+NS group restored the epithelial thickness. Area percent of alpha-smooth muscle actin expression was significantly decreased in radiation group at the third day followed by significant increase at the seventh day. However, all treatment groups showed significant increase in alpha-smooth muscle actin at the third day, which decreased to normal level at the seventh day. Our study demonstrated the efficiency of Aloe vera and silver nanoparticles in enhancing ulcer healing after irradiation.
[Mh] Termos MeSH primário: Aloe/química
Raios gama/efeitos adversos
Nanopartículas Metálicas/uso terapêutico
Úlceras Orais/tratamento farmacológico
Úlceras Orais/etiologia
Lesões Experimentais por Radiação/tratamento farmacológico
Prata/uso terapêutico
[Mh] Termos MeSH secundário: Ácido Acético
Actinas/análise
Administração Tópica
Animais
Células Epiteliais/efeitos dos fármacos
Células Epiteliais/efeitos da radiação
Fibroblastos/efeitos dos fármacos
Imuno-Histoquímica
Masculino
Camundongos
Microscopia Eletrônica de Transmissão
Úlceras Orais/patologia
Lesões Experimentais por Radiação/patologia
Valores de Referência
Reprodutibilidade dos Testes
Fatores de Tempo
Resultado do Tratamento
Cicatrização/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RANDOMIZED CONTROLLED TRIAL
[Nm] Nome de substância:
0 (Actins); 0 (alpha-smooth muscle actin, mouse); 3M4G523W1G (Silver); Q40Q9N063P (Acetic Acid)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180307
[Lr] Data última revisão:
180307
[Sb] Subgrupo de revista:D; IM
[Da] Data de entrada para processamento:180208
[St] Status:MEDLINE


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[PMID]:29253824
[Au] Autor:Swain B; Shin D; Joo SY; Ahn NK; Lee CG; Yoon JH
[Ad] Endereço:Institute for Advanced Engineering (IAE), Advanced Materials & Processing Center, Yongin-Si, 449-863, Republic of Korea. Electronic address: swain@iae.re.kr.
[Ti] Título:Synthesis of submicron silver powder from scrap low-temperature co-fired ceramic an e-waste: Understanding the leaching kinetics and wet chemistry.
[So] Source:Chemosphere;194:793-802, 2018 Mar.
[Is] ISSN:1879-1298
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The current study focuses on the understanding of leaching kinetics of metal in the LTCC in general and silver leaching in particular along with wet chemical reduction involving silver nanoparticle synthesis. Followed by metal leaching, the silver was selectively precipitated using HCl as AgCl. The precipitated AgCl was dissolved in ammonium hydroxide and reduced to pure silver metal nanopowder (NPs) using hydrazine as a reductant. Polyvinylpyrrolidone (PVP) used as a stabilizer and Polyethylene glycol (PEG) used as reducing reagent as well as stabilizing reagent to control size and shape of the Ag NPs. An in-depth investigation indicated a first-order kinetics model fits well with high accuracy among all possible models. Activation energy required for the first order reaction was 21.242 kJ mol for Silver. PVP and PEG 1% each together provide better size control over silver nanoparticle synthesis using 0.4 M hydrazine as reductant, which provides relatively regular morphology in comparison to their individual application. The investigation revealed that the waste LTCC (an industrial e-waste) can be recycled through the reported process even in industrial scale. The novelty of reported recycling process is simplicity, versatile and eco-efficiency through which waste LTCC recycling can address various issues like; (i) industrial waste disposal (ii) synthesis of silver nanoparticles from waste LTCC (iii) circulate metal economy within a closed loop cycle in the industrial economies where resources are scarce, altogether.
[Mh] Termos MeSH primário: Cerâmica/química
Nanopartículas Metálicas/química
Reciclagem
Prata/química
[Mh] Termos MeSH secundário: Resíduos Industriais/análise
Cinética
Pós/síntese química
Pós/química
Temperatura Ambiente
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Industrial Waste); 0 (Powders); 3M4G523W1G (Silver)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180228
[Lr] Data última revisão:
180228
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171219
[St] Status:MEDLINE


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[PMID]:29182243
[Au] Autor:Liao X; Yang F; Li H; So PK; Yao Z; Xia W; Sun H
[Ad] Endereço:MOE Key Laboratory of Bioinorganic and Synthetic Chemistry, School of Chemistry, Sun Yat-sen University , Guangzhou, China , 510275.
[Ti] Título:Targeting the Thioredoxin Reductase-Thioredoxin System from Staphylococcus aureus by Silver Ions.
[So] Source:Inorg Chem;56(24):14823-14830, 2017 Dec 18.
[Is] ISSN:1520-510X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The thioredoxin system, which is composed of NADPH, thioredoxin reductase (TrxR), and thioredoxin (Trx), is one of the major disulfide reductase systems used by bacteria against oxidative stress. In particular, this reductase system is crucial for the survival of the pathogenic bacterium Staphylococcus aureus, which lacks a natural glutathione/glutaredoxin (Grx) system. Although silver ions and silver-containing materials have been used as antibacterial agents for centuries, the antibacterial mechanism of silver is not well-understood. Herein, we demonstrate that silver ions bind to the active sites of S. aureus TrxR and Trx with dissociation constants of 1.4 ± 0.1 µM and 15.0 ± 5.0 µM and stoichiometries of 1 and 2 Ag ions per protein, respectively. Importantly, silver ion binding leads to oligomerization and functional disruption of TrxR as well as Trx. Silver also depleted intracellular thiol levels in S. aureus, disrupting bacterial thiol-redox homeostasis. Our study provides new insights into the antibacterial mechanism of silver ions. Moreover, the Trx and TrxR system might serve as a feasible target for the design of antibacterial drugs.
[Mh] Termos MeSH primário: Antibacterianos/farmacologia
Proteínas de Bactérias/antagonistas & inibidores
Prata/farmacologia
Staphylococcus aureus/efeitos dos fármacos
Tiorredoxina Dissulfeto Redutase/antagonistas & inibidores
Tiorredoxinas/antagonistas & inibidores
[Mh] Termos MeSH secundário: Proteínas de Bactérias/metabolismo
Domínio Catalítico/efeitos dos fármacos
Seres Humanos
Modelos Moleculares
Multimerização Proteica/efeitos dos fármacos
Infecções Estafilocócicas/tratamento farmacológico
Infecções Estafilocócicas/microbiologia
Staphylococcus aureus/metabolismo
Tiorredoxina Dissulfeto Redutase/metabolismo
Tiorredoxinas/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Bacterial Proteins); 3M4G523W1G (Silver); 52500-60-4 (Thioredoxins); EC 1.8.1.9 (Thioredoxin-Disulfide Reductase)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180228
[Lr] Data última revisão:
180228
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171129
[St] Status:MEDLINE
[do] DOI:10.1021/acs.inorgchem.7b01904


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[PMID]:29372919
[Au] Autor:Karaballi RA; Merchant S; Power SR; Brosseau CL
[Ad] Endereço:Department of Chemistry, Saint Mary's University, Halifax, Nova Scotia B3H 3C3, Canada. christa.brosseau@smu.ca.
[Ti] Título:Electrochemical surface-enhanced Raman spectroscopy (EC-SERS) study of the interaction between protein aggregates and biomimetic membranes.
[So] Source:Phys Chem Chem Phys;20(6):4513-4526, 2018 Feb 07.
[Is] ISSN:1463-9084
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Human diseases characterized by the uncontrolled deposition of insoluble extracellular protein aggregates are collectively referred to as amyloidoses. Such diseases include Alzheimer's, Parkinson's, Huntington's, and prion disease. In Alzheimer's disease, it is believed that amyloid-ß proteins may be responsible for pore and defect formation within cellular membranes, leading to a breakdown of cellular homeostasis causing eventual neuronal death. This theory is referred to as the amyloid pore hypothesis of Alzheimer's disease. In this work, the interaction between a model amyloid-forming protein (insulin) and a biomimetic membrane was studied at the molecular level. Protein at different stages of aggregation was allowed to interact with a biomimetic membrane formed on a nanostructured substrate using Langmuir-Blodgett/Langmuir-Schaefer deposition. Electrochemical surface-enhanced Raman spectroscopy (EC-SERS) was used to monitor the molecular level changes occurring as a result of this interaction. Based on the results it was observed that oligomers and protofibrils caused the most significant membrane deterioration whilst native protein appeared to play a protective role. To the best of our knowledge, this work represents the first EC-SERS investigation of protein aggregate-biomembrane interactions, and highlights the usefulness of this tool for studying complex biomolecular interactions.
[Mh] Termos MeSH primário: Amiloide/química
Biomimética
Insulina/química
Bicamadas Lipídicas/química
Agregados Proteicos
[Mh] Termos MeSH secundário: Amiloide/metabolismo
Técnicas Eletroquímicas
Eletrodos
Seres Humanos
Insulina/metabolismo
Bicamadas Lipídicas/metabolismo
Nanopartículas Metálicas/química
Microscopia Eletrônica de Varredura
Microscopia Eletrônica de Transmissão
Nefelometria e Turbidimetria
Prata/química
Análise Espectral Raman
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Amyloid); 0 (Insulin); 0 (Lipid Bilayers); 0 (Protein Aggregates); 3M4G523W1G (Silver)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180226
[Lr] Data última revisão:
180226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180127
[St] Status:MEDLINE
[do] DOI:10.1039/c7cp06838g


  10 / 18838 MEDLINE  
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[PMID]:29206032
[Au] Autor:Rajendran K; Anwar A; Khan NA; Siddiqui R
[Ad] Endereço:Department of Biological Sciences, School of Science and Technology, Sunway University , Bandar Sunway 47500, Malaysia.
[Ti] Título:Brain-Eating Amoebae: Silver Nanoparticle Conjugation Enhanced Efficacy of Anti-Amoebic Drugs against Naegleria fowleri.
[So] Source:ACS Chem Neurosci;8(12):2626-2630, 2017 12 20.
[Is] ISSN:1948-7193
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The overall aim of this study was to determine whether conjugation with silver nanoparticles enhances effects of available drugs against primary amoebic meningoencephalitis due to Naegleria fowleri. Amphotericin B, Nystatin, and Fluconazole were conjugated with silver nanoparticles, and synthesis was confirmed using UV-visible spectrophotometry. Atomic force microscopy determined their size in range of 20-100 nm. To determine amoebicidal effects, N. fowleri were incubated with drugs-conjugated silver nanoparticles, silver nanoparticles alone, and drugs alone. The findings revealed that silver nanoparticles conjugation significantly enhanced antiamoebic effects of Nystatin and Amphotericin B but not Fluconazole at micromolar concentrations, compared with the drugs alone. For the first time, our findings showed that silver nanoparticle conjugation enhances efficacy of antiamoebic drugs against N. fowleri. Given the rarity of the disease and challenges in developing new drugs, it is hoped that modifying existing drugs to enhance their antiamoebic effects is a useful avenue that holds promise in improving the treatment of brain-eating amoebae infection due to N. fowleri.
[Mh] Termos MeSH primário: Amebicidas/administração & dosagem
Nanopartículas Metálicas/administração & dosagem
Naegleria fowleri/efeitos dos fármacos
Naegleria fowleri/fisiologia
Nanoconjugados/administração & dosagem
Nanoconjugados/química
Prata/administração & dosagem
[Mh] Termos MeSH secundário: Sobrevivência Celular/efeitos dos fármacos
Relação Dose-Resposta a Droga
Combinação de Medicamentos
Sinergismo Farmacológico
Fluconazol/administração & dosagem
Nanopartículas Metálicas/química
Nanopartículas Metálicas/ultraestrutura
Naegleria fowleri/citologia
Nanocápsulas/administração & dosagem
Nanocápsulas/química
Nanocápsulas/ultraestrutura
Nanoconjugados/ultraestrutura
Nistatina/administração & dosagem
Tamanho da Partícula
Prata/química
Taxa de Sobrevida
Resultado do Tratamento
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Amebicides); 0 (Drug Combinations); 0 (Nanocapsules); 0 (Nanoconjugates); 1400-61-9 (Nystatin); 3M4G523W1G (Silver); 8VZV102JFY (Fluconazole)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180226
[Lr] Data última revisão:
180226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171206
[St] Status:MEDLINE
[do] DOI:10.1021/acschemneuro.7b00430



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