[PMID]: | 27896311 |
[Au] Autor: | Nakano T; Mackay SM; Wui Tan E; Dani KM; Wickens J |
[Ad] Endereço: | Neurobiology Research Unit, Okinawa Institute of Science and Technology Graduate University, Onna-son 904-0412, Okinawa, Japan; Department of Neurobiology, Institute of Biomedical and Health Sciences, Hiroshima University, Hiroshima 734-8553, Japan. |
[Ti] Título: | Interfacing with Neural Activity via Femtosecond Laser Stimulation of Drug-Encapsulating Liposomal Nanostructures. |
[So] Source: | eNeuro;3(6), 2016 Nov-Dec. |
[Is] ISSN: | 2373-2822 |
[Cp] País de publicação: | United States |
[La] Idioma: | eng |
[Ab] Resumo: | External control over rapid and precise release of chemicals in the brain potentially provides a powerful interface with neural activity. Optical manipulation techniques, such as optogenetics and caged compounds, enable remote control of neural activity and behavior with fine spatiotemporal resolution. However, these methods are limited to chemicals that are naturally present in the brain or chemically suitable for caging. Here, we demonstrate the ability to interface with neural functioning via a wide range of neurochemicals released by stimulating loaded liposomal nanostructures with femtosecond lasers. Using a commercial two-photon microscope, we released inhibitory or excitatory neurochemicals to evoke subthreshold and suprathreshold changes in membrane potential in a live mouse brain slice. The responses were repeatable and could be controlled by adjusting laser stimulation characteristics. We also demonstrate the release of a wider range of chemicals-which previously were impossible to release by optogenetics or uncaging-including synthetic analogs of naturally occurring neurochemicals. In particular, we demonstrate the release of a synthetic receptor-specific agonist that exerts physiological effects on long-term synaptic plasticity. Further, we show that the loaded liposomal nanostructures remain functional for weeks in a live mouse. In conclusion, we demonstrate new techniques capable of interfacing with live neurons, and extendable to applications. |
[Mh] Termos MeSH primário: |
Lasers Lipossomos Nanoestruturas Neurônios/efeitos dos fármacos Neurotransmissores/administração & dosagem
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[Mh] Termos MeSH secundário: |
2,3,4,5-Tetra-Hidro-7,8-Di-Hidroxi-1-Fenil-1H-3-Benzazepina/administração & dosagem Animais Corpo Estriado/efeitos dos fármacos Corpo Estriado/fisiologia Sistemas de Liberação de Medicamentos Compostos de Ouro Proteínas de Fluorescência Verde/genética Proteínas de Fluorescência Verde/metabolismo Hipocampo/efeitos dos fármacos Hipocampo/fisiologia Masculino Potenciais da Membrana/efeitos dos fármacos Camundongos Endogâmicos C57BL Camundongos Transgênicos Microscopia Muscimol/administração & dosagem Plasticidade Neuronal/efeitos dos fármacos Plasticidade Neuronal/fisiologia Neurônios/fisiologia Técnicas de Patch-Clamp Receptores de Dopamina D1/agonistas Receptores de Dopamina D1/genética Receptores de Dopamina D1/metabolismo Técnicas de Cultura de Tecidos
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[Pt] Tipo de publicação: | JOURNAL ARTICLE |
[Nm] Nome de substância:
| 0 (Drd1a protein, mouse); 0 (Gold Compounds); 0 (Liposomes); 0 (Neurotransmitter Agents); 0 (Receptors, Dopamine D1); 0 (enhanced green fluorescent protein); 147336-22-9 (Green Fluorescent Proteins); 2763-96-4 (Muscimol); 67287-49-4 (2,3,4,5-Tetrahydro-7,8-dihydroxy-1-phenyl-1H-3-benzazepine) |
[Em] Mês de entrada: | 1710 |
[Cu] Atualização por classe: | 171025 |
[Lr] Data última revisão:
| 171025 |
[Sb] Subgrupo de revista: | IM |
[Da] Data de entrada para processamento: | 161130 |
[St] Status: | MEDLINE |
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