Base de dados : MEDLINE
Pesquisa : D01.538 [Categoria DeCS]
Referências encontradas : 874 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 88 ir para página                         

  1 / 874 MEDLINE  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28668464
[Au] Autor:Gill R; McCabe MJ; Rosenspire AJ
[Ad] Endereço:Department of Immunology, Microbiology and Biochemistry, Wayne State University, Detroit, MI, United States.
[Ti] Título:Low level exposure to inorganic mercury interferes with B cell receptor signaling in transitional type 1 B cells.
[So] Source:Toxicol Appl Pharmacol;330:22-29, 2017 Sep 01.
[Is] ISSN:1096-0333
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Mercury (Hg) has been implicated as a factor contributing to autoimmune disease in animal models and humans. However the mechanism by which this occurs has remained elusive. Since the discovery of B cells it has been appreciated by immunologists that during the normal course of B cell development, some immature B cells must be generated that produce immunoglobulin reactive to self-antigens (auto-antibodies). However in the course of normal development, the vast majority of immature auto-reactive B cells are prevented from maturing by processes collectively known as tolerance. Autoimmune disease arises when these mechanisms of tolerance are disrupted. In the B cell compartment, it is firmly established that tolerance depends in part upon negative selection of self-reactive immature (transitional type 1) B cells. In these cells negative selection depends upon signals generated by the B Cell Receptor (BCR), in the sense that those T1 B cells who's BCRs most strongly bind to, and so generate the strongest signals to self-antigens are neutralized. In this report we have utilized multicolor phosphoflow cytometry to show that in immature T1 B cells Hg attenuates signal generation by the BCR through mechanisms that may involve Lyn, a key tyrosine kinase in the BCR signal transduction pathway. We suggest that exposure to low, environmentally relevant levels of Hg, disrupts tolerance by interfering with BCR signaling in immature B cells, potentially leading to the appearance of mature auto-reactive B cells which have the ability to contribute to auto-immune disease.
[Mh] Termos MeSH primário: Linfócitos B/metabolismo
Compostos de Mercúrio/toxicidade
Receptores de Antígenos de Linfócitos B/efeitos dos fármacos
Receptores de Antígenos de Linfócitos B/metabolismo
Transdução de Sinais/efeitos dos fármacos
[Mh] Termos MeSH secundário: Animais
Linfócitos B/efeitos dos fármacos
Linhagem Celular
Membrana Celular/efeitos dos fármacos
Membrana Celular/metabolismo
Feminino
Imunoglobulinas/metabolismo
Imuno-Histoquímica
Linfócitos/efeitos dos fármacos
Sistema de Sinalização das MAP Quinases/efeitos dos fármacos
Camundongos
Camundongos Endogâmicos BALB C
Baço/citologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Immunoglobulins); 0 (Mercury Compounds); 0 (Receptors, Antigen, B-Cell)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170824
[Lr] Data última revisão:
170824
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170703
[St] Status:MEDLINE


  2 / 874 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28641222
[Au] Autor:Tang H; Duan Y; Zhu C; Cai T; Li C; Cai L
[Ad] Endereço:Key Laboratory of Energy Thermal Conversion and Control of Ministry of Education, School of Energy and Environment, Southeast University, Nanjing, Jiangsu 210096, China.
[Ti] Título:Theoretical evaluation on selective adsorption characteristics of alkali metal-based sorbents for gaseous oxidized mercury.
[So] Source:Chemosphere;184:711-719, 2017 Oct.
[Is] ISSN:1879-1298
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Alkali metal-based sorbents are potential for oxidized mercury (Hg ) selective adsorption but show hardly effect to elemental mercury (Hg ) in flue gas. Density functional theory (DFT) was employed to investigate the Hg and HgCl adsorption mechanism over alkali metal-based sorbents, including calcium oxide (CaO), magnesium oxide (MgO), potassium chloride (KCl) and sodium chloride (NaCl). Hg was found to weakly interact with CaO (001), MgO (001), KCl (001) and NaCl (001) surfaces while HgCl was effectively adsorbed on top-O and top-Cl sites. Charge transfer and bond population were calculated to discuss the covalency and ionicity of HgCl bonding with the adsorption sites. The partial density of states (PDOS) analysis manifests that HgCl strongly interacts with surface sites through the orbital hybridizations between Hg and top O or Cl. Frontier molecular orbital (FMO) energy and Mulliken electronegativity are introduced as the quantitative criteria to evaluate the reactivity of mercury species and alkali metal-based sorbents. HgCl is identified as a Lewis acid and more reactive than Hg . The Lewis basicity of the four alkali metal-based sorbents is predicted as the increasing order: NaCl < MgO < KCl < CaO, in consistence with the trend of HgCl adsorption energies.
[Mh] Termos MeSH primário: Mercúrio/química
Metais Alcalinos/química
Modelos Químicos
[Mh] Termos MeSH secundário: Adsorção
Compostos de Cálcio
Gases
Óxido de Magnésio
Cloreto de Mercúrio
Compostos de Mercúrio
Óxidos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Calcium Compounds); 0 (Gases); 0 (Mercury Compounds); 0 (Metals, Alkali); 0 (Oxides); 3A3U0GI71G (Magnesium Oxide); 53GH7MZT1R (Mercuric Chloride); C7X2M0VVNH (lime); FXS1BY2PGL (Mercury); IY191986AO (mercuric oxide)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171010
[Lr] Data última revisão:
171010
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170623
[St] Status:MEDLINE


  3 / 874 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28571847
[Au] Autor:Balland-Bolou-Bi C; Turc B; Alphonse V; Bousserrhine N
[Ad] Endereço:Université Paris-Est Créteil Val de Marne, Institute of Ecology and Environmental Sciences of Paris (UMR 7618), 61 avenue du Général De Gaulle, 94010 Créteil cedex, France. Electronic address: Clarisse.bolou-bi@u-pec.fr.
[Ti] Título:Impact of microbial communities from tropical soils on the mobilization of trace metals during dissolution of cinnabar ore.
[So] Source:J Environ Sci (China);56:122-130, 2017 Jun.
[Is] ISSN:1001-0742
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Biodissolution experiments on cinnabar ore (mercury sulphide and other sulphide minerals, such as pyrite) were performed with microorganisms extracted directly from soil. These experiments were carried out in closed systems under aerobic and anaerobic conditions with 2 different soils sampled in French Guyana. The two main objectives of this study were (1) to quantify the ability of microorganisms to mobilize metals (Fe, Al, Hg) during the dissolution of cinnabar ore, and (2) to identify the links between the type and chemical properties of soils, environmental parameters such as season and the strategies developed by indigenous microorganisms extracted from tropical natural soils to mobilize metals. Results indicate that microbial communities extracted directly from various soils are able to (1) survive in the presence of cinnabar ore, as indicated by consumption of carbon sources and, (2) leach Hg from cinnabar in oxic and anoxic dissolution experiments via the acidification of the medium and the production of low molecular mass organic acids (LMMOAs). The dissolution rate of cinnabar in aerobic conditions with microbial communities ranged from 4.8×10 to 2.6×10 µmol/m /day and was independent of the metabolites released by the microorganisms. In addition, these results suggest an indirect action by the microorganisms in the cinnabar dissolution. Additionally, because iron is a key element in the dynamics of Hg, microbes were stimulated by the presence of this metal, and microbes released LMMOAs that leached iron from iron-bearing minerals, such as pyrite and oxy-hydroxide of iron, in the mixed cinnabar ore.
[Mh] Termos MeSH primário: Monitoramento Ambiental
Compostos de Mercúrio
Metais/toxicidade
Microbiologia do Solo
Poluentes do Solo/toxicidade
[Mh] Termos MeSH secundário: Clima Tropical
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Mercury Compounds); 0 (Metals); 0 (Soil Pollutants); ZI0T668SF1 (cinnabar)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170630
[Lr] Data última revisão:
170630
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170603
[St] Status:MEDLINE


  4 / 874 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28536007
[Au] Autor:Zhang BB; Li WK; Hou WY; Luo Y; Shi JZ; Li C; Wei LX; Liu J
[Ad] Endereço:Key Lab for Pharmacology of Ministry of Education, Joint International Research Laboratory of Ethnomedicine of Ministry of Education, Zunyi Medical University, Zunyi 563000, China.
[Ti] Título:Zuotai and HgS differ from HgCl and methyl mercury in Hg accumulation and toxicity in weanling and aged rats.
[So] Source:Toxicol Appl Pharmacol;331:76-84, 2017 Sep 15.
[Is] ISSN:1096-0333
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Mercury sulfides are used in Ayurvedic medicines, Tibetan medicines, and Chinese medicines for thousands of years and are still used today. Cinnabar (α-HgS) and metacinnabar (ß-HgS) are different from mercury chloride (HgCl ) and methylmercury (MeHg) in their disposition and toxicity. Whether such scenario applies to weanling and aged animals is not known. To address this question, weanling (21d) and aged (450d) rats were orally given Zuotai (54% ß-HgS, 30mg/kg), HgS (α-HgS, 30mg/kg), HgCl (34.6mg/kg), or MeHg (MeHgCl, 3.2mg/kg) for 7days. Accumulation of Hg in kidney and liver, and the toxicity-sensitive gene expressions were examined. Animal body weight gain was decreased by HgCl and to a lesser extent by MeHg, but unaltered after Zuotai and HgS. HgCl and MeHg produced dramatic tissue Hg accumulation, increased kidney (kim-1 and Ngal) and liver (Ho-1) injury-sensitive gene expressions, but such changes are absent or mild after Zuotai and HgS. Aged rats were more susceptible than weanling rats to Hg toxicity. To examine roles of transporters in Hg accumulation, transporter gene expressions were examined. The expression of renal uptake transporters Oat1, Oct2, and Oatp4c1 and hepatic Oatp2 was decreased, while the expression of renal efflux transporter Mrp2, Mrp4 and Mdr1b was increased following HgCl and MeHg, but unaffected by Zuotai and HgS. Thus, Zuotai and HgS differ from HgCl and MeHg in producing tissue Hg accumulation and toxicity, and aged rats are more susceptible than weanling rats. Transporter expression could be adaptive means to reduce tissue Hg burden.
[Mh] Termos MeSH primário: Envelhecimento/efeitos dos fármacos
Medicamentos de Ervas Chinesas/toxicidade
Cloreto de Mercúrio/toxicidade
Compostos de Mercúrio/toxicidade
Compostos de Metilmercúrio/toxicidade
[Mh] Termos MeSH secundário: Administração Oral
Envelhecimento/metabolismo
Animais
Animais Recém-Nascidos
Medicamentos de Ervas Chinesas/administração & dosagem
Medicamentos de Ervas Chinesas/metabolismo
Cloreto de Mercúrio/administração & dosagem
Cloreto de Mercúrio/metabolismo
Mercúrio/administração & dosagem
Mercúrio/metabolismo
Mercúrio/toxicidade
Compostos de Mercúrio/administração & dosagem
Compostos de Mercúrio/metabolismo
Compostos de Metilmercúrio/administração & dosagem
Compostos de Metilmercúrio/metabolismo
Ratos
Ratos Sprague-Dawley
Desmame
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Drugs, Chinese Herbal); 0 (Mercury Compounds); 0 (Methylmercury Compounds); 0 (zuotai); 53GH7MZT1R (Mercuric Chloride); FXS1BY2PGL (Mercury)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170904
[Lr] Data última revisão:
170904
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170525
[St] Status:MEDLINE


  5 / 874 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28484136
[Au] Autor:Watanabe T; Hayashi T; Matsuda R; Akiyama H; Teshima R
[Ad] Endereço:National Institute of Health Sciences.
[Ti] Título:Surveillance of Total Mercury and Methylmercury Concentrations in Retail Fish.
[So] Source:Shokuhin Eiseigaku Zasshi;58(2):80-85, 2017.
[Is] ISSN:1882-1006
[Cp] País de publicação:Japan
[La] Idioma:jpn
[Ab] Resumo:Most fish samples contain methylmercury, that the concentrations very greatly according to the fish species. To avoid the adverse health effects of methylmercury while retaining the benefits provided by fish consumption, it is important to select suitable fish species and to control the amount of the fish intake. We surveyed the concentrations of total mercury and methylmercury in 210 retail fish samples classified into 19 fish species by using validated analytical methods. The results of this survey were as follows. The total mercury and methylmercury concentrations were higher than 1 mg/kg in some samples of swordfish and bluefin tuna, which are large predatory fish species. In bluefin tuna and yellowtail, total mercury and methylmercury concentrations in farm-raised fish were lower than those in natural fish. There was a positive correlation between total mercury concentration and methylmercury concentration. Our results indicate that a cut-off value of 0.3 mg/kg total mercury in the screening of fish samples would increase the effectiveness of inspection.
[Mh] Termos MeSH primário: Produtos Pesqueiros/análise
Peixes/metabolismo
Análise de Alimentos/métodos
Compostos de Mercúrio/análise
Compostos de Metilmercúrio/análise
[Mh] Termos MeSH secundário: Animais
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Mercury Compounds); 0 (Methylmercury Compounds)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:171016
[Lr] Data última revisão:
171016
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170510
[St] Status:MEDLINE
[do] DOI:10.3358/shokueishi.58.80


  6 / 874 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28381770
[Au] Autor:Charanyaa S; Belur PD; Regupathi I
[Ad] Endereço:Department of Chemical Engineering, National Institute of Technology Karnataka.
[Ti] Título:A New Strategy to Refine Crude Indian Sardine Oil.
[So] Source:J Oleo Sci;66(5):425-434, 2017 May 01.
[Is] ISSN:1347-3352
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:Current work aims to develop a refining process for removing phospholipids, free fatty acids (FFA), and metal ions without affecting n-3 polyunsaturated fatty acid (n-3 PUFA) esters present in the crude Indian sardine oil. Sardine oil was subjected to degumming with various acids (orthophosphoric acid, acetic acid, and lactic acid), conventional and membrane assisted deacidification using various solvents (methanol, ethanol, propanol and butanol) and bleaching with bleaching agents (GAC, activated earth and bentonite) and all the process parameters were further optimized. Degumming with 5%(w/w) ortho phosphoric acid, two stage solvent extraction with methanol at 1:1 (w/w) in each stage and bleaching with 3% (w/w) activated charcoal loading, at 80ºC for 10 minutes resulted in the reduction of phospholipid content to 5.66 ppm from 612.66 ppm, FFA to 0.56% from 5.64% with the complete removal of iron and mercury. Under these conditions, the obtained bleached oil showed an enhancement of n-3 PUFA from 16.39 % (11.19 Eicosapentaenoic acid (EPA) + 5.20 Docosahexaenoic acid (DHA)) to 17.91 % (11.81 EPA + 6.1 DHA). Replacing conventional solvent extraction with membrane deacidification using microporous, hydrophobic polytetrafluoroethylene membrane (PTFE), resulted in a lesser solvent residue (0.25% (w/w)) in the deacidified oil. In view of lack of reports on refining of n-3 PUFA rich marine oils without concomitant loss of n-3 PUFA, this report is significant.
[Mh] Termos MeSH primário: Ésteres/análise
Ácidos Graxos Ômega-3/análise
Óleos de Peixe/análise
Óleos de Peixe/química
[Mh] Termos MeSH secundário: Clareadores
Carvão Vegetal
Ácidos Graxos não Esterificados/isolamento & purificação
Íons
Compostos de Ferro/isolamento & purificação
Compostos de Mercúrio/isolamento & purificação
Fosfolipídeos/isolamento & purificação
Ácidos Fosfóricos
Politetrafluoretileno
Solventes
Temperatura Ambiente
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bleaching Agents); 0 (Esters); 0 (Fatty Acids, Nonesterified); 0 (Fatty Acids, Omega-3); 0 (Fish Oils); 0 (Ions); 0 (Iron Compounds); 0 (Mercury Compounds); 0 (Phospholipids); 0 (Phosphoric Acids); 0 (Solvents); 16291-96-6 (Charcoal); 9002-84-0 (Polytetrafluoroethylene); E4GA8884NN (phosphoric acid)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170407
[St] Status:MEDLINE
[do] DOI:10.5650/jos.ess16164


  7 / 874 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28379072
[Au] Autor:Branco V; Caito S; Farina M; Teixeira da Rocha J; Aschner M; Carvalho C
[Ad] Endereço:a Research Institute for Medicines (iMed.ULisboa), Faculdade de Farmácia , Universidade de Lisboa , Lisboa , Portugal.
[Ti] Título:Biomarkers of mercury toxicity: Past, present, and future trends.
[So] Source:J Toxicol Environ Health B Crit Rev;20(3):119-154, 2017.
[Is] ISSN:1521-6950
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Mercury (Hg) toxicity continues to represent a global health concern. Given that human populations are mostly exposed to low chronic levels of mercurial compounds (methylmercury through fish, mercury vapor from dental amalgams, and ethylmercury from vaccines), the need for more sensitive and refined tools to assess the effects and/or susceptibility to adverse metal-mediated health risks remains. Traditional biomarkers, such as hair or blood Hg levels, are practical and provide a reliable measure of exposure, but given intra-population variability, it is difficult to establish accurate cause-effect relationships. It is therefore important to identify and validate biomarkers that are predictive of early adverse effects prior to adverse health outcomes becoming irreversible. This review describes the predominant biomarkers used by toxicologists and epidemiologists to evaluate exposure, effect and susceptibility to Hg compounds, weighing on their advantages and disadvantages. Most importantly, and in light of recent findings on the molecular mechanisms underlying Hg-mediated toxicity, potential novel biomarkers that might be predictive of toxic effect are presented, and the applicability of these parameters in risk assessment is examined.
[Mh] Termos MeSH primário: Exposição Ambiental/efeitos adversos
Poluentes Ambientais/toxicidade
Compostos de Mercúrio/toxicidade
Mercúrio/toxicidade
Compostos de Metilmercúrio/toxicidade
[Mh] Termos MeSH secundário: Animais
Biomarcadores/análise
Seres Humanos
Medição de Risco
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Biomarkers); 0 (Environmental Pollutants); 0 (Mercury Compounds); 0 (Methylmercury Compounds); FXS1BY2PGL (Mercury)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170616
[Lr] Data última revisão:
170616
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170406
[St] Status:MEDLINE
[do] DOI:10.1080/10937404.2017.1289834


  8 / 874 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28339347
[Au] Autor:Bridges CC; Zalups RK
[Ad] Endereço:a Mercer University School of Medicine , Division of Basic Medical Sciences , Macon , Georgia , USA.
[Ti] Título:The aging kidney and the nephrotoxic effects of mercury.
[So] Source:J Toxicol Environ Health B Crit Rev;20(2):55-80, 2017.
[Is] ISSN:1521-6950
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Owing to advances in modern medicine, life expectancies are lengthening and leading to an increase in the population of older individuals. The aging process leads to significant alterations in many organ systems, with the kidney being particularly susceptible to age-related changes. Within the kidney, aging leads to ultrastructural changes such as glomerular and tubular hypertrophy, glomerulosclerosis, and tubulointerstitial fibrosis, which may compromise renal plasma flow (RPF) and glomerular filtration rate (GFR). These alterations may reduce the functional reserve of the kidneys, making them more susceptible to pathological events when challenged or stressed, such as following exposure to nephrotoxicants. An important and prevalent environmental toxicant that induces nephrotoxic effects is mercury (Hg). Since exposure of normal kidneys to mercuric ions might induce glomerular and tubular injury, aged kidneys, which may not be functioning at full capacity, may be more sensitive to the effects of Hg than normal kidneys. Age-related renal changes and the effects of Hg in the kidney have been characterized separately. However, little is known regarding the influence of nephrotoxicants, such as Hg, on aged kidneys. The purpose of this review was to summarize known findings related to exposure of aged and diseased kidneys to the environmentally relevant nephrotoxicant Hg.
[Mh] Termos MeSH primário: Envelhecimento
Poluentes Ambientais/toxicidade
Rim/efeitos dos fármacos
Compostos de Mercúrio/toxicidade
Mercúrio/toxicidade
Compostos Organomercúricos/toxicidade
[Mh] Termos MeSH secundário: Animais
Seres Humanos
Rim/fisiologia
Rim/fisiopatologia
Nefropatias/induzido quimicamente
Camundongos
Ratos
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Environmental Pollutants); 0 (Mercury Compounds); 0 (Organomercury Compounds); FXS1BY2PGL (Mercury)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:171011
[Lr] Data última revisão:
171011
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170325
[St] Status:MEDLINE
[do] DOI:10.1080/10937404.2016.1243501


  9 / 874 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28319158
[Au] Autor:Gutiérrez-de-Juan V; López de Davalillo S; Fernández-Ramos D; Barbier-Torres L; Zubiete-Franco I; Fernández-Tussy P; Simon J; Lopitz-Otsoa F; de Las Heras J; Iruzubieta P; Arias-Loste MT; Villa E; Crespo J; Andrade R; Lucena MI; Varela-Rey M; Lu SC; Mato JM; Delgado TC; Martínez-Chantar ML
[Ad] Endereço:CIC bioGUNE (Center for Cooperative Research in Biosciences), Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBERehd), Derio, Bizkaia, Spain.
[Ti] Título:A morphological method for ammonia detection in liver.
[So] Source:PLoS One;12(3):e0173914, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Hyperammonemia is a metabolic condition characterized by elevated levels of ammonia and a common event in acute liver injury/failure and chronic liver disease. Even though hepatic ammonia levels are potential predictive factors of patient outcome, easy and inexpensive methods aiming at the detection of liver ammonia accumulation in the clinical setting remain unavailable. Thus, herein we have developed a morphological method, based on the utilization of Nessler´s reagent, to accurately and precisely detect the accumulation of ammonia in biological tissue. We have validated our method against a commercially available kit in mouse tissue samples and, by using this modified method, we have confirmed the hepatic accumulation of ammonia in clinical and animal models of acute and chronic advanced liver injury as well as in the progression of fatty liver disease. Overall, we propose a morphological method for ammonia detection in liver that correlates well with the degree of liver disease severity and therefore can be potentially used to predict patient outcome.
[Mh] Termos MeSH primário: Amônia/metabolismo
Técnicas Citológicas/métodos
Iodetos/metabolismo
Fígado/citologia
Fígado/metabolismo
Compostos de Mercúrio/metabolismo
[Mh] Termos MeSH secundário: Adolescente
Adulto
Idoso
Animais
Pré-Escolar
Seres Humanos
Fígado/lesões
Fígado/patologia
Masculino
Camundongos
Meia-Idade
Hepatopatia Gordurosa não Alcoólica/metabolismo
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Iodides); 0 (Mercury Compounds); 7664-41-7 (Ammonia); 7N83S6IU0F (potassium tetraiodomercurate)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170825
[Lr] Data última revisão:
170825
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170321
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0173914


  10 / 874 MEDLINE  
              first record previous record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28273537
[Au] Autor:Baldi F; Gallo M; Daniele S; Battistel D; Faleri C; Kodre A; Arcon I
[Ad] Endereço:Dipartimento di Scienze Molecolari e Nanosistemi, University Cà Foscari Venezia, Via Torino 155, 30172, Mestre Venezia, Italy. Electronic address: baldi@unive.it.
[Ti] Título:An extracellular polymeric substance quickly chelates mercury(II) with N-heterocyclic groups.
[So] Source:Chemosphere;176:296-304, 2017 Jun.
[Is] ISSN:1879-1298
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A strain of Klebsiella oxytoca DSM 29614 is grown on sodium citrate in the presence of 50 mg l of Hg as Hg(NO ) . During growth, the strain produces an extracellular polymeric substance (EPS), constituted by a mixture of proteins and a specific exopolysaccharide. The protein components, derived from the outer membrane of cells, are co-extracted with the extracellular exopolysaccharide using ethanol. The extracted EPS contains 7.5% of Hg (total amount). This indicates that EPS is an excellent material for the biosorption of Hg , through chemical complexation with the EPS components. The binding capacity of these species towards Hg is studied by cyclic voltammetry, and Hg L -edge XANES and EXAFS spectroscopy. The results found indicate that Hg is mainly bound to the nitrogen of the imidazole ring or other N-heterocycle compounds. The hydroxyl moities of sugars and/or the carboxyl groups of two glucuronic acids in the polysaccharide can also play an important role in sequestring Hg ions. However, N-heterocyclic groups of proteins bind Hg faster than hydroxyl and carboxyl groups of the polysaccharide.
[Mh] Termos MeSH primário: Quelantes/química
Compostos Heterocíclicos/química
Klebsiella oxytoca/metabolismo
Compostos de Mercúrio/análise
Nitratos/análise
Polissacarídeos Bacterianos/química
Poluentes Químicos da Água/análise
[Mh] Termos MeSH secundário: Proteínas de Bactérias/química
Quelantes/metabolismo
Klebsiella oxytoca/ultraestrutura
Compostos de Mercúrio/metabolismo
Modelos Teóricos
Nitratos/metabolismo
Polissacarídeos Bacterianos/metabolismo
Ligação Proteica
Poluentes Químicos da Água/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Chelating Agents); 0 (Heterocyclic Compounds); 0 (Mercury Compounds); 0 (Nitrates); 0 (Polysaccharides, Bacterial); 0 (Water Pollutants, Chemical); 24670-15-3 (mercury nitrate)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170517
[Lr] Data última revisão:
170517
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170309
[St] Status:MEDLINE



página 1 de 88 ir para página                         
   


Refinar a pesquisa
  Base de dados : MEDLINE Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde