Base de dados : MEDLINE
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  1 / 3222 MEDLINE  
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[PMID]:29329094
[Au] Autor:Sentkowska A; Pyrzynska K
[Ad] Endereço:University of Warsaw, Heavy Ion Laboratory, Pasteura 5A, 02-093 Warsaw, Poland. Electronic address: sentkowska@slcj.uw.edu.pl.
[Ti] Título:Hydrophilic interaction liquid chromatography in the speciation analysis of selenium.
[So] Source:J Chromatogr B Analyt Technol Biomed Life Sci;1074-1075:8-15, 2018 Feb 01.
[Is] ISSN:1873-376X
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The hydrophilic interaction liquid chromatography (HILIC) coupled to mass spectrometry was employed to study retention behavior of selected selenium compounds using two different HILIC stationary phases: silica and zwitterionic. Two organic solvents - acetonitrile and methanol - were compared as a component of mobile phase. Separation parameters such as a content of organic modifier, the eluent pH and inorganic buffer concentration were investigated. Based on all observations, methanol seems to be beneficial for the separation of studied compounds. The optimal HILIC separation method involved silica column and eluent composed of 85% MeOH and CH COONH (8 mM, pH 7) was compared to RP method in terms of time of the single run, the separation efficiency and limit of detection.
[Mh] Termos MeSH primário: Cromatografia Líquida/métodos
Compostos de Selênio
Selênio
[Mh] Termos MeSH secundário: Acetonitrilos/química
Interações Hidrofóbicas e Hidrofílicas
Metanol/química
Cebolas/química
Extratos Vegetais/química
Folhas de Planta/química
Selênio/análise
Selênio/química
Selênio/classificação
Compostos de Selênio/análise
Compostos de Selênio/química
Compostos de Selênio/classificação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Acetonitriles); 0 (Plant Extracts); 0 (Selenium Compounds); H6241UJ22B (Selenium); Y4S76JWI15 (Methanol); Z072SB282N (acetonitrile)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180305
[Lr] Data última revisão:
180305
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180113
[St] Status:MEDLINE


  2 / 3222 MEDLINE  
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[PMID]:29187424
[Au] Autor:Evans SO; Khairuddin PF; Jameson MB
[Ad] Endereço:Biomedical Research Unit, School of Science, University of Waikato, Hamilton, New Zealand.
[Ti] Título:Optimising Selenium for Modulation of Cancer Treatments.
[So] Source:Anticancer Res;37(12):6497-6509, 2017 12.
[Is] ISSN:1791-7530
[Cp] País de publicação:Greece
[La] Idioma:eng
[Ab] Resumo:Selenium is an essential trace element involved in many biological processes that are mediated through, at least, 25 selenoproteins expressed in humans. Extensive study of selenium compounds has demonstrated growth inhibition of malignant cells in a vast array of experimental models. Moreover combining selenium with conventional cancer therapy has yielded promising results in both preclinical studies and a cohort of human trials. The aim of this review is to highlight the current research evaluating the role of selenium compounds in combination with chemotherapy and radiation. Pharmacodymanic mechanisms responsible for the differential effects of the commonly studied compounds on healthy and malignant cells are presented and the pertinent in vitro and in vivo data summarised. The clinical utility of this approach is discussed both in terms of anti-tumour efficacy and toxicity prevention. Finally a case is made for novel trial designs to facilitate rapid progression into pivotal studies.
[Mh] Termos MeSH primário: Antineoplásicos/uso terapêutico
Neoplasias/terapia
Radioterapia/métodos
Compostos de Selênio/uso terapêutico
[Mh] Termos MeSH secundário: Animais
Antioxidantes/uso terapêutico
Diarreia/etiologia
Diarreia/prevenção & controle
Sinergismo Farmacológico
Seres Humanos
Radioterapia/efeitos adversos
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Antineoplastic Agents); 0 (Antioxidants); 0 (Selenium Compounds)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171211
[Lr] Data última revisão:
171211
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171201
[St] Status:MEDLINE


  3 / 3222 MEDLINE  
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[PMID]:29031452
[Au] Autor:Wen J; Ma C; Huo P; Liu X; Wei M; Liu Y; Yao X; Ma Z; Yan Y
[Ad] Endereço:School of the Environment and Safety Engineering, Jiangsu University, Zhenjiang 212013, China. Electronic address: wenjiangshu@sina.cn.
[Ti] Título:Construction of vesicle CdSe nano-semiconductors photocatalysts with improved photocatalytic activity: Enhanced photo induced carriers separation efficiency and mechanism insight.
[So] Source:J Environ Sci (China);60:98-107, 2017 Oct.
[Is] ISSN:1001-0742
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Visible-light-driven photocatalysis as a green technology has attracted a lot of attention due to its potential applications in environmental remediation. Vesicle CdSe nano-semiconductor photocatalyst are successfully prepared by a gas template method and characterized by a variety of methods. The vesicle CdSe nano-semiconductors display enhanced photocatalytic performance for the degradation of tetracycline hydrochloride, the photodegradation rate of 78.824% was achieved by vesicle CdSe, which exhibited an increase of 31.779% compared to granular CdSe. Such an exceptional photocatalytic capability can be attributed to the unique structure of the vesicle CdSe nano-semiconductor with enhanced light absorption ability and excellent carrier transport capability. Meanwhile, the large surface area of the vesicle CdSe nano-semiconductor can increase the contact probability between catalyst and target and provide more surface-active centers. The photocatalytic mechanisms are analyzed by active species quenching. It indicates that h and O are the main active species which play a major role in catalyzing environmental toxic pollutants. Simultaneously, the vesicle CdSe nano-semiconductor had high efficiency and stability.
[Mh] Termos MeSH primário: Compostos de Cádmio/química
Modelos Químicos
Processos Fotoquímicos
Compostos de Selênio/química
Semicondutores
[Mh] Termos MeSH secundário: Nanoestruturas
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cadmium Compounds); 0 (Selenium Compounds)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171019
[Lr] Data última revisão:
171019
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171017
[St] Status:MEDLINE


  4 / 3222 MEDLINE  
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[PMID]:28818774
[Au] Autor:Mohamadi E; Moghaddasi M; Farahbakhsh A; Kazemi A
[Ad] Endereço:Department of Chemical Engineering, Shahrood Branch, Islamic Azad University, Shahrood, Iran.
[Ti] Título:A quantum-dot-based fluoroassay for detection of food-borne pathogens.
[So] Source:J Photochem Photobiol B;174:291-297, 2017 Sep.
[Is] ISSN:1873-2682
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:Evaluation of the distribution capability of food-borne pathogens existing in food products by taking the advantage of quantum dots (QDs) for their photoluminescence properties was carried out. Bacteria namely Escherichia coli (E. coli) labelled with CdSe-QDs were examined both on an Agar nutrient and ground fish substrates in order to observe their growth rate in different environments in the Lab. A sample with an appropriate concentration ratio 10 CFU/mL of bacteria/CdSe-QDs was empirically selected from the samples which were grown on the Agar containing plates. The selected sample was also tested on a ground fish substrate as a real food sample. The bacterial growth was observed under the irradiation of UV light and the growth patterns were investigated for 3 successive days. The growth patterns indicated that E. coli can stay alive and can be distributed on food products so that the growth can be easily monitored. This approach makes bacterial growth on food products detectable so that it can be used as a bacteria-QD assay for an easy detection of food borne pathogens grown on a food sample.
[Mh] Termos MeSH primário: Escherichia coli/isolamento & purificação
Microbiologia de Alimentos
Nanotecnologia/métodos
Pontos Quânticos/química
[Mh] Termos MeSH secundário: Animais
Compostos de Cádmio/química
Escherichia coli/metabolismo
Peixes/microbiologia
Corantes Fluorescentes/química
Corantes Fluorescentes/metabolismo
Compostos de Selênio/química
Espectrometria de Fluorescência
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cadmium Compounds); 0 (Fluorescent Dyes); 0 (Selenium Compounds); A7F646JC5C (cadmium selenide)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171012
[Lr] Data última revisão:
171012
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170819
[St] Status:MEDLINE


  5 / 3222 MEDLINE  
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[PMID]:28763969
[Au] Autor:Bierla K; Suzuki N; Ogra Y; Szpunar J; Lobinski R
[Ad] Endereço:CNRS/UPPA, Institute for Analytical Sciences and Physical Chemistry for the Environment and Materials (IPREM), UMR 5254, Hélioparc, F-64053 Pau, France.
[Ti] Título:Identification and determination of selenohomolanthionine - The major selenium compound in Torula yeast.
[So] Source:Food Chem;237:1196-1201, 2017 Dec 15.
[Is] ISSN:0308-8146
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Torula yeast (Candida utilis) was found to metabolize selenium in a totally different way to Brewer's yeast (S. cerevisiae) leading to the biosynthesis of selenohomolanthionine (SeHLan), a major selenium compound accounting for 60-80% of the total selenium. The identity of SeHLan was confirmed by retention time matching in hydrophilic ion interaction chromatography (HILIC) with inductively coupled plasma mass spectrometric detection (ICP MS) using a custom synthesized standard molecule and by HILIC - Orbitrap MS and MS-MS fragmentation. Selenohomolanthionine escapes the current assays for the organic character of Se-rich yeast based on the protein-bound selenomethionine determination. A HILIC - ICP MS method was developed for the quantitative determination of selenohomolanthionine in yeast supplements with a detection limit of 146ng/g.
[Mh] Termos MeSH primário: Cryptococcus/química
[Mh] Termos MeSH secundário: Cromatografia Líquida de Alta Pressão
Homocisteína/análogos & derivados
Espectrometria de Massas
Compostos Organosselênicos
Selênio
Compostos de Selênio
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (4,4'-selenobis(2-aminobutanoic acid)); 0 (Organoselenium Compounds); 0 (Selenium Compounds); 0LVT1QZ0BA (Homocysteine); H6241UJ22B (Selenium)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171030
[Lr] Data última revisão:
171030
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170803
[St] Status:MEDLINE


  6 / 3222 MEDLINE  
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[PMID]:28739737
[Au] Autor:Sun L; Zhang J; Yang Q; Si Y; Liu Y; Wang Q; Han F; Huang Z
[Ad] Endereço:Department of Nutrition and Metabolism, National Institute for Nutrition and Health, Chinese Center for Disease Control and Prevention, Beijing, P.R. China.
[Ti] Título:Synergistic Effects of SAM and Selenium Compounds on Proliferation, Migration and Adhesion of HeLa Cells.
[So] Source:Anticancer Res;37(8):4433-4441, 2017 08.
[Is] ISSN:1791-7530
[Cp] País de publicação:Greece
[La] Idioma:eng
[Ab] Resumo:BACKGROUND/AIM: To determine the antitumor activities and molecular mechanism of selenium compounds in HeLa cells. MATERIALS AND METHODS: Western blotting was used to detect ERK and AKT activation in HeLa cells induced by selenium compounds selenomethionine (SeMet), methylselenocysteine (MeSeCys) and methylseleninic acids (MeSeA). Using MTT, wound-healing and Matrigel adhesion assays, the antitumor effects of SAM and selenium compounds were evaluated in HeLa cells. RESULTS: MeSeA inhibited ERK and AKT signaling pathways and suppressed the proliferation (p<0.05 vs. HeLa control), migration (p<0.05 vs. HeLa control) and adhesion (p<0.01 vs. HeLa control) of HeLa cells. MeSeCys and SeMet inhibited AKT signaling pathways and the migration (p<0.05 vs. HeLa control) and adhesion (p<0.01 vs. HeLa control) of HeLa cells. The synergistic action of MeSeA with SAM led to a statistically significant inhibition of proliferation, migration and adhesion of HeLa cells. CONCLUSION: MeSeA, MeSeCys and SeMet exert different antitumor activities by inhibiting ERK and AKT signaling pathways. The combination of MeSeA and SAM exhibited better antitumor effects compared to the other treatments.
[Mh] Termos MeSH primário: Sistema de Sinalização das MAP Quinases/efeitos dos fármacos
Proteínas Proto-Oncogênicas c-akt/metabolismo
S-Adenosilmetionina/administração & dosagem
Compostos de Selênio/administração & dosagem
Neoplasias do Colo do Útero/tratamento farmacológico
[Mh] Termos MeSH secundário: Animais
Adesão Celular/efeitos dos fármacos
Movimento Celular/efeitos dos fármacos
Proliferação Celular/efeitos dos fármacos
Sinergismo Farmacológico
Feminino
Regulação Neoplásica da Expressão Gênica/efeitos dos fármacos
Células HeLa
Seres Humanos
Camundongos
S-Adenosilmetionina/farmacologia
Compostos de Selênio/farmacologia
Neoplasias do Colo do Útero/metabolismo
Ensaios Antitumorais Modelo de Xenoenxerto
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Selenium Compounds); 7LP2MPO46S (S-Adenosylmethionine); EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170906
[Lr] Data última revisão:
170906
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170726
[St] Status:MEDLINE


  7 / 3222 MEDLINE  
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[PMID]:28717110
[Au] Autor:Hoang VAT; Sakamoto M; Yamamoto M
[Ad] Endereço:Department of Basic Medical Science, National Institute for Minamata Disease.
[Ti] Título:Mercury and selenium levels, and their molar ratios in several species of commercial shrimp in Japan regarding the health risk of methylmercury exposure.
[So] Source:J Toxicol Sci;42(4):509-517, 2017.
[Is] ISSN:1880-3989
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:The Japanese shrimp industry depends on importing shrimp from other countries. However, little information is available on mercury speciation and selenium (Se) concentrations in commercial shrimp available in Japan. The present study determined the concentrations of total mercury (T-Hg), methylmercury (MeHg), and Se in the muscles (wet weight) of imported and domestic commercial shrimp from Kumamoto and Kagoshima prefectures to obtain information for assessing the risk of MeHg exposure. The median concentrations of T-Hg, MeHg and Se in shrimp imported from three different countries were, respectively: black tiger shrimp (n = 18), 15.8, 14.4, and 415 ng/g; Vannamei shrimp (n = 25), 11.4, 11.2, and 292 ng/g; and white shrimp (n = 26), 26.8, 26.1, and 396 ng/g. There were significant differences in T-Hg and MeHg concentrations between shrimp imported from different countries. The median concentrations of T-Hg, MeHg and Se in shrimp of Japanese origin were, respectively: Shiba shrimp (n = 10), 15.9, 15.0, and 270 ng/g; Kuruma shrimp (n = 10), 79.9, 75.9, and 390 ng/g; and Ashiaka shrimp (n = 10), 36.1, 34.1, and 303 ng/g. The percentages of MeHg in T-Hg were between 90% and 99%, with MeHg levels in the imported and domestic commercial shrimp lower than the Japanese regulation of 300 ng/g for fish. The mean Se/T-Hg molar ratios (16-160) were comparatively higher than those previously reported in fish. Overall, this survey suggests that shrimp commercially available in Japan will not pose a particularly high risk regarding MeHg exposure to consumers.
[Mh] Termos MeSH primário: Poluentes Ambientais/análise
Análise de Alimentos
Contaminação de Alimentos/análise
Mercúrio/análise
Compostos de Metilmercúrio/análise
Penaeidae/química
Compostos de Selênio/análise
Frutos do Mar/análise
[Mh] Termos MeSH secundário: Animais
Japão
Risco
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Environmental Pollutants); 0 (Methylmercury Compounds); 0 (Selenium Compounds); FXS1BY2PGL (Mercury)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171005
[Lr] Data última revisão:
171005
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170719
[St] Status:MEDLINE
[do] DOI:10.2131/jts.42.509


  8 / 3222 MEDLINE  
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[PMID]:28619032
[Au] Autor:Manshian BB; Martens TF; Kantner K; Braeckmans K; De Smedt SC; Demeester J; Jenkins GJS; Parak WJ; Pelaz B; Doak SH; Himmelreich U; Soenen SJ
[Ad] Endereço:Biomedical NMR Unit/MoSAIC, KU Leuven Campus Gasthuisberg, Herestraat 49, 3000, Louvain, Belgium. bella.manshian@kuleuven.be.
[Ti] Título:The role of intracellular trafficking of CdSe/ZnS QDs on their consequent toxicity profile.
[So] Source:J Nanobiotechnology;15(1):45, 2017 Jun 15.
[Is] ISSN:1477-3155
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Nanoparticle interactions with cellular membranes and the kinetics of their transport and localization are important determinants of their functionality and their biological consequences. Understanding these phenomena is fundamental for the translation of such NPs from in vitro to in vivo systems for bioimaging and medical applications. Two CdSe/ZnS quantum dots (QD) with differing surface functionality (NH or COOH moieties) were used here for investigating the intracellular uptake and transport kinetics of these QDs. RESULTS: In water, the COOH- and NH -QDs were negatively and positively charged, respectively, while in serum-containing medium the NH -QDs were agglomerated, whereas the COOH-QDs remained dispersed. Though intracellular levels of NH - and COOH-QDs were very similar after 24 h exposure, COOH-QDs appeared to be continuously internalised and transported by endosomes and lysosomes, while NH -QDs mainly remained in the lysosomes. The results of (intra)cellular QD trafficking were correlated to their toxicity profiles investigating levels of reactive oxygen species (ROS), mitochondrial ROS, autophagy, changes to cellular morphology and alterations in genes involved in cellular stress, toxicity and cytoskeletal integrity. The continuous flux of COOH-QDs perhaps explains their higher toxicity compared to the NH -QDs, mainly resulting in mitochondrial ROS and cytoskeletal remodelling which are phenomena that occur early during cellular exposure. CONCLUSIONS: Together, these data reveal that although cellular QD levels were similar after 24 h, differences in the nature and extent of their cellular trafficking resulted in differences in consequent gene alterations and toxicological effects.
[Mh] Termos MeSH primário: Autofagia/efeitos dos fármacos
Compostos de Cádmio/toxicidade
Pontos Quânticos/toxicidade
Espécies Reativas de Oxigênio/metabolismo
Compostos de Selênio/toxicidade
Sulfetos/toxicidade
Compostos de Zinco/toxicidade
[Mh] Termos MeSH secundário: Compostos de Cádmio/análise
Compostos de Cádmio/metabolismo
Linhagem Celular
Regulação da Expressão Gênica/efeitos dos fármacos
Seres Humanos
Lisossomos/efeitos dos fármacos
Lisossomos/metabolismo
Mitocôndrias/efeitos dos fármacos
Mitocôndrias/metabolismo
Pontos Quânticos/análise
Pontos Quânticos/metabolismo
Compostos de Selênio/análise
Compostos de Selênio/metabolismo
Sulfetos/análise
Sulfetos/metabolismo
Compostos de Zinco/análise
Compostos de Zinco/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cadmium Compounds); 0 (Reactive Oxygen Species); 0 (Selenium Compounds); 0 (Sulfides); 0 (Zinc Compounds); A7F646JC5C (cadmium selenide); KPS085631O (zinc sulfide)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170913
[Lr] Data última revisão:
170913
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170617
[St] Status:MEDLINE
[do] DOI:10.1186/s12951-017-0279-0


  9 / 3222 MEDLINE  
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[PMID]:28614013
[Au] Autor:Ijaz N; Fitzgerald D
[Ad] Endereço:Specialty Doctor in Dermatology, Dermatology Department, Salford Royal Hospital, Manchester M6 8HD.
[Ti] Título:Seborrhoeic dermatitis.
[So] Source:Br J Hosp Med (Lond);78(6):C88-C91, 2017 Jun 02.
[Is] ISSN:1750-8460
[Cp] País de publicação:England
[La] Idioma:eng
[Mh] Termos MeSH primário: Corticosteroides/uso terapêutico
Antifúngicos/uso terapêutico
Dermatite Seborreica/terapia
Inibidores Enzimáticos/uso terapêutico
Ceratolíticos/uso terapêutico
Fototerapia
[Mh] Termos MeSH secundário: Administração Cutânea
Lesões Encefálicas Traumáticas/epidemiologia
Inibidores de Calcineurina
Dermatite Seborreica/epidemiologia
Dermatite Seborreica/imunologia
Dermatomicoses/epidemiologia
Dieta
Dermatoses Faciais
Seres Humanos
Imidazóis/uso terapêutico
Malassezia
Compostos Organometálicos/uso terapêutico
Doença de Parkinson/epidemiologia
Piridinas/uso terapêutico
Fatores de Risco
Dermatoses do Couro Cabeludo
Compostos de Selênio/uso terapêutico
Traumatismos da Medula Espinal/epidemiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Adrenal Cortex Hormones); 0 (Antifungal Agents); 0 (Calcineurin Inhibitors); 0 (Enzyme Inhibitors); 0 (Imidazoles); 0 (Keratolytic Agents); 0 (Organometallic Compounds); 0 (Pyridines); 0 (Selenium Compounds); 7GBN705NH1 (imidazole); R953O2RHZ5 (pyrithione zinc); Z69D9E381Q (selenium disulfide)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170615
[St] Status:MEDLINE
[do] DOI:10.12968/hmed.2017.78.6.C88


  10 / 3222 MEDLINE  
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[PMID]:28601593
[Au] Autor:Gilbert-López B; Dernovics M; Moreno-González D; Molina-Díaz A; García-Reyes JF
[Ad] Endereço:Analytical Chemistry Research Group, Department of Physical and Analytical Chemistry, University of Jaén, E-23071 Jaén, Spain.
[Ti] Título:Detection of over 100 selenium metabolites in selenized yeast by liquid chromatography electrospray time-of-flight mass spectrometry.
[So] Source:J Chromatogr B Analyt Technol Biomed Life Sci;1060:84-90, 2017 Aug 15.
[Is] ISSN:1873-376X
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The characterization of the selenometabolome of Selenized(Se)-yeast, that is the fraction of water soluble low-molecular weight Se-metabolites produced in Se-yeast is of paramount interest to expand the knowledge on the composition of this food supplement. In this work, we have applied liquid chromatography electrospray time-of-flight mass spectrometry (LC-TOFMS) to search for Se-species from the low molecular weight range fraction of the selenized yeast used for food supplements. Prior to LC-TOFMS, sample treatment consisted of ultrasound assisted water extraction followed by size exclusion fractionation assisted with off-line inductively coupled plasma mass spectrometry detection of isotope Se. The fraction corresponding to low-molecular weight species was subjected to LC-TOFMS using electrospray ionization in the positive ion mode. The detection of the suspected selenized species has been based on the information obtained from accurate mass measurements of both the protonated molecules and fragments from in-source CID fragmentation; along with the characteristic isotope pattern exhibited by the presence of Se. The approach enables the detection of 103 selenized species, most of them not previously reported, in the range from ca. 300-650Da. Besides the detection of selenium species, related sulphur derivate metabolites were detected based on the accurate mass shift due to the substitution of sulphur and selenium.
[Mh] Termos MeSH primário: Cromatografia Líquida de Alta Pressão/métodos
Saccharomyces cerevisiae/metabolismo
Compostos de Selênio/análise
Compostos de Selênio/metabolismo
Selênio/metabolismo
Espectrometria de Massas por Ionização por Electrospray/métodos
[Mh] Termos MeSH secundário: Compostos de Selênio/química
Enxofre/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Selenium Compounds); 70FD1KFU70 (Sulfur); H6241UJ22B (Selenium)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170915
[Lr] Data última revisão:
170915
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170612
[St] Status:MEDLINE



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