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[PMID]:28456645
[Au] Autor:Wang W; Bai Q; Liang T; Bai H; Liu X
[Ad] Endereço:Key Laboratory of Synthetic and Biological Colloids, Ministry of Education, School of Chemical and Material Engineering, Jiangnan University, Wuxi 214122, PR China. Electronic address: ww12230098@sina.com.
[Ti] Título:Preparation of amino-functionalized regenerated cellulose membranes with high catalytic activity.
[So] Source:Int J Biol Macromol;102:944-951, 2017 Sep.
[Is] ISSN:1879-0003
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The modification of regenerated cellulose (RC) membranes was carried out by using silane coupling agents presenting primary and secondary amino-groups. The grafting of the amino groups onto the modified cellulose molecule was confirmed by X-ray photoelectron spectroscopies and C nuclear magnetic resonance spectroscopic analyses. The crystallinity of the cellulose membranes (CM) decreased after chemical modification as indicated by the X-ray diffraction results. Moreover, a denser structure was observed at the surface and cross section of the modified membranes by SEM images. The contact angle measurements showed that the silane coupling treatment enhanced the hydrophobicity of the obtained materials. Then the catalytic properties of two types of modified membranes were studied in a batch process by evaluating their catalytic performance in a Knoevenagel condensation. The results indicated that the cellulose membrane grafted with many secondary amines exhibited a better catalytic activity compared to the one grafted only by primary amines. In addition, the compact structure of the modified membranes permitted their application in a pervaporation catalytic membrane reactor. Therefore, functional CM that prepared in this paper represented a promising material in the field of industrial catalysis.
[Mh] Termos MeSH primário: Celulose/química
Membranas Artificiais
[Mh] Termos MeSH secundário: Catálise
Silanos/química
Propriedades de Superfície
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Membranes, Artificial); 0 (Silanes); 9004-34-6 (Cellulose)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170501
[St] Status:MEDLINE


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[PMID]:28459245
[Au] Autor:Magalhães APR; Decurcio RA; Ojeda GPD; Teixeira TR; Cardoso PC
[Ad] Endereço:Professor of Operative Dentistry, Brazilian Dental Association, Goiânia, Brazil.
[Ti] Título:Does Post-etching Cleaning Influence Bond Strength of Lithium Disilicate Laminate Veneers?
[So] Source:Compend Contin Educ Dent;38(5):e9-e12, 2017 May.
[Is] ISSN:2158-1797
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:This paper aims to present a clinical case of laminate veneers in lithium disilicate (LD) reinforced ceramic, complemented with scanning electron microscopy and energy dispersive x-ray spectroscopy analysis of this ceramic's surfaces submitted to some of the different post-etching cleaning protocols proposed by literature. Many dentists are applying all-ceramic restorations, especially veneers, with the most applied material being LD, due to its good mechanical properties, esthetics, and ability to adhere to tooth substrate. The cementation process is critical to clinical success of indirect restorations. Combination of hydrofluoric acid and silane application on the inner surface of ceramic has been the most recommended method to increase bond strength between ceramic and tooth substrate. As a result of surface corrosion, residual subproducts are formed and precipitate on the ceramic surface, affecting the bonding process to the resin cement. Elimination of residue is recommended in the literature and can be done via 3 common techniques: thorough washing with air/water spray for 30 seconds, 37% phosphoric acid for 60 seconds, or ultrasonic bath for 4 to 10 minutes. Little information on LD ceramics post-etching cleaning methods is available in the literature. For this ceramic, the thorough washing with air/water spray for 30 seconds appeared to remove most of the residue without damaging the material. Being also the most practical and easiest of the reviewed procedures, it was, therefore, the protocol of choice for the clinical cases presented in this paper with success after 18 months.
[Mh] Termos MeSH primário: Ataque Ácido Dentário/métodos
Colagem Dentária
Porcelana Dentária
Facetas Dentárias
[Mh] Termos MeSH secundário: Adulto
Cerâmica
Feminino
Seres Humanos
Ácido Fluorídrico
Cimentos de Resina/química
Silanos
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Resin Cements); 0 (Silanes); 0 (lithia disilicate); 12001-21-7 (Dental Porcelain); RGL5YE86CZ (Hydrofluoric Acid)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180308
[Lr] Data última revisão:
180308
[Sb] Subgrupo de revista:D
[Da] Data de entrada para processamento:170502
[St] Status:MEDLINE


  3 / 5576 MEDLINE  
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[PMID]:29338033
[Au] Autor:Dreyer-Andersen N; Almeida AS; Jensen P; Kamand M; Okarmus J; Rosenberg T; Friis SD; Martínez Serrano A; Blaabjerg M; Kristensen BW; Skrydstrup T; Gramsbergen JB; Vieira HLA; Meyer M
[Ad] Endereço:Department of Neurobiology Research, Institute of Molecular Medicine, University of Southern Denmark, Odense, Denmark.
[Ti] Título:Intermittent, low dose carbon monoxide exposure enhances survival and dopaminergic differentiation of human neural stem cells.
[So] Source:PLoS One;13(1):e0191207, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Exploratory studies using human fetal tissue have suggested that intrastriatal transplantation of dopaminergic neurons may become a future treatment for patients with Parkinson's disease. However, the use of human fetal tissue is compromised by ethical, regulatory and practical concerns. Human stem cells constitute an alternative source of cells for transplantation in Parkinson's disease, but efficient protocols for controlled dopaminergic differentiation need to be developed. Short-term, low-level carbon monoxide (CO) exposure has been shown to affect signaling in several tissues, resulting in both protection and generation of reactive oxygen species. The present study investigated the effect of CO produced by a novel CO-releasing molecule on dopaminergic differentiation of human neural stem cells. Short-term exposure to 25 ppm CO at days 0 and 4 significantly increased the relative content of ß-tubulin III-immunoreactive immature neurons and tyrosine hydroxylase expressing catecholaminergic neurons, as assessed 6 days after differentiation. Also the number of microtubule associated protein 2-positive mature neurons had increased significantly. Moreover, the content of apoptotic cells (Caspase3) was reduced, whereas the expression of a cell proliferation marker (Ki67) was left unchanged. Increased expression of hypoxia inducible factor-1α and production of reactive oxygen species (ROS) in cultures exposed to CO may suggest a mechanism involving mitochondrial alterations and generation of ROS. In conclusion, the present procedure using controlled, short-term CO exposure allows efficient dopaminergic differentiation of human neural stem cells at low cost and may as such be useful for derivation of cells for experimental studies and future development of donor cells for transplantation in Parkinson's disease.
[Mh] Termos MeSH primário: Monóxido de Carbono/administração & dosagem
Células-Tronco Neurais/citologia
Células-Tronco Neurais/efeitos dos fármacos
[Mh] Termos MeSH secundário: Apoptose/efeitos dos fármacos
Monóxido de Carbono/metabolismo
Diferenciação Celular/efeitos dos fármacos
Proliferação Celular/efeitos dos fármacos
Sobrevivência Celular/efeitos dos fármacos
Células Cultivadas
Citocinas/metabolismo
Neurônios Dopaminérgicos/citologia
Neurônios Dopaminérgicos/efeitos dos fármacos
Neurônios Dopaminérgicos/metabolismo
Seres Humanos
Subunidade alfa do Fator 1 Induzível por Hipóxia/metabolismo
Células-Tronco Neurais/metabolismo
Espécies Reativas de Oxigênio/metabolismo
Silanos/administração & dosagem
Silanos/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Cytokines); 0 (HIF1A protein, human); 0 (Hypoxia-Inducible Factor 1, alpha Subunit); 0 (Reactive Oxygen Species); 0 (Silanes); 7U1EE4V452 (Carbon Monoxide)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180220
[Lr] Data última revisão:
180220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180117
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0191207


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[PMID]:28992485
[Au] Autor:Mercado DF; Bracco LLB; Arques A; Gonzalez MC; Caregnato P
[Ad] Endereço:Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas (INIFTA), CCT-La Plata-CONICET, Universidad Nacional de La Plata, Casilla de Correo 16, Sucursal 4, 1900, La Plata, Argentina.
[Ti] Título:Reaction kinetics and mechanisms of organosilicon fungicide flusilazole with sulfate and hydroxyl radicals.
[So] Source:Chemosphere;190:327-336, 2018 Jan.
[Is] ISSN:1879-1298
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Flusilazole is an organosilane fungicide used for treatments in agriculture and horticulture for control of diseases. The reaction kinetics and mechanism of flusilazole with sulfate and hydroxyl radicals were studied. The rate constant of the radicals with the fungicide were determined by laser flash photolysis of peroxodisulfate and hydrogen peroxide. The results were 2.0 × 10 s M for the reaction of the fungicide with HO and 4.6 × 10 s M for the same reaction with SO radicals. The absorption spectra of organic intermediates detected by laser flash photolysis of S O with flusilazole, were identified as α-aminoalkyl and siloxyl radicals and agree very well with those estimated employing the time-dependent density functional theory with explicit account for bulk solvent effects. In the continuous photolysis experiments, performed by photo-Fenton reaction of the fungicide, the main degradation products were: (bis(4-fluorophenyl)-hydroxy-methylsilane) and the non-toxic silicic acid, diethyl bis(trimethylsilyl) ester, in ten and twenty minutes of reaction, respectively.
[Mh] Termos MeSH primário: Radical Hidroxila/química
Silanos/química
Sulfatos/química
Triazóis/química
[Mh] Termos MeSH secundário: Radicais Livres/química
Fungicidas Industriais/química
Peróxido de Hidrogênio/química
Cinética
Fotólise
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Free Radicals); 0 (Fungicides, Industrial); 0 (Silanes); 0 (Sulfates); 0 (Triazoles); 3352-57-6 (Hydroxyl Radical); BBX060AN9V (Hydrogen Peroxide); F3WG2VVD87 (flusilazole)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180214
[Lr] Data última revisão:
180214
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171010
[St] Status:MEDLINE


  5 / 5576 MEDLINE  
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[PMID]:28449584
[Au] Autor:Alghunaim A; Brink ET; Newby EY; Zhang Newby BM
[Ad] Endereço:Department of Chemical and Biomolecular Engineering, The University of Akron, 200 E. Buchtel Commons, Akron, Ohio 44325-3906.
[Ti] Título:Retention of poly(N-isopropylacrylamide) on 3-aminopropyltriethoxysilane.
[So] Source:Biointerphases;12(2):02C405, 2017 04 27.
[Is] ISSN:1559-4106
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Silane coupling agents are commonly employed to link an organic polymer to an inorganic substrate. One of the widely utilized coupling agents is 3-aminopropyltriethoxy silane (APTES). In this study, the authors investigated the ability of APTES to retain thermo-responsive poly(N-isopropylacrylamide) (pNIPAAm) on hydroxylated surfaces such as glass. For comparison purposes, the authors also evaluated the retention behaviors of (1) polystyrene, which likely has weaker van der Waals interactions and acid-base interactions (contributed by hydrogen-bonding) with APTES, on APTES as well as (2) pNIPAAm on two other silane coupling agents, which have similar structures to APTES, but exhibit less interaction with pNIPAAm. Under our processing conditions, the stronger interactions, particularly hydrogen bonding, between pNIPAAm and APTES were found to contribute substantially to the retention of pNIPAAm on the APTES modified surface, especially on the cured APTES layer when the interpenetration was minimal or nonexistent. On the noncured APTES layer, the formation of an APTES-pNIPAAm interpenetrating network resulted in the retention of thicker pNIPAAm films. As demonstrated by water contact angles [i.e., 7°-15° higher at 40 °C, the temperature above the lower critical solution temperature (LCST) of 32 °C for pNIPAAm, as compared to those at 25 °C] and cell attachment and detachment behaviors (i.e., attached/spread at 37 °C, above LCST; detached at 20 °C, below LCST), the retained pNIPAAm layer (6-15 nm), on both noncured and cured APTES, exhibited thermo-responsive behavior. The results in this study illustrate the simplicity of using the coupling/adhesion promoting ability of APTES to retain pNIPAAm films on hydroxylated substrates, which exhibit faster cell sheet detachment (≤30 min) as compared to pNIPAAm brushes (in hours) prepared using tedious and costly grafting approaches. The use of adhesion promoters to retain pNIPAAm provides an affordable alternative to current thermo-responsive supports for cell sheet engineering and stem cell therapy applications.
[Mh] Termos MeSH primário: Resinas Acrílicas/química
Propilaminas/química
Silanos/química
[Mh] Termos MeSH secundário: Adsorção
Solventes/química
Propriedades de Superfície
Temperatura Ambiente
Termodinâmica
Água/química
Molhabilidade
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.; RESEARCH SUPPORT, N.I.H., EXTRAMURAL
[Nm] Nome de substância:
0 (Acrylic Resins); 0 (Propylamines); 0 (Silanes); 0 (Solvents); 059QF0KO0R (Water); 25189-55-3 (poly-N-isopropylacrylamide); L8S6UBW552 (amino-propyl-triethoxysilane)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180201
[Lr] Data última revisão:
180201
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170429
[St] Status:MEDLINE
[do] DOI:10.1116/1.4982248


  6 / 5576 MEDLINE  
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[PMID]:28832225
[Au] Autor:Hanafi-Bojd MY; Moosavian Kalat SA; Taghdisi SM; Ansari L; Abnous K; Malaekeh-Nikouei B
[Ad] Endereço:a Cellular and Molecular Research Center, Department of Pharmacology, School of Medicine , Birjand University of Medical Sciences , Birjand , Iran.
[Ti] Título:MUC1 aptamer-conjugated mesoporous silica nanoparticles effectively target breast cancer cells.
[So] Source:Drug Dev Ind Pharm;44(1):13-18, 2018 Jan.
[Is] ISSN:1520-5762
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:In the present study, we developed aptamer (Apt) conjugated mesoporous silica nanoparticles (MSNs) for specific delivery of epirubicin (EPI) to breast cancer cells. MSNs were synthesized and functionalized with 3-mercaptopropyltrimethoxysilane (3-MPTMS), followed by MUC1 aptamer conjugation through disulfide bonds. The nanoparticles were analyzed by transmission electron microscopy (TEM), particle size analyzer, zeta potential, elemental analysis (CHNS), aptamer conjugation efficiency, drug loading efficiency, and drug release profile. Cell uptake and in vitro cytotoxicity of different formulations were performed. The results of MSNs characterization confirmed spherical nanoparticles with thiol functional groups. Particle size of obtained nanoparticles was 163 nm in deionized water. After conjugation of MUC1 aptamer and EPI loading (MSN-MUC1-EPI), particle size increased to 258 nm. The aptamer conjugation to MSNs with disulfide bonds were confirmed using gel retardation assay. Cellular uptake studies revealed better cell uptake of MSN-MUC1-EPI compared to MSN-EPI. Moreover, cytotoxicity study results in MCF7 cell lines showed improved cytotoxicity of MSN-MUC1-EPI in comparison with MSN-EPI or EPI at the same concentration of drug. These results exhibited that MSN-MUC1-EPI has the potential for targeted drug delivery into MUC1 positive breast cancer cells to improve drug efficacy and alleviate side effects.
[Mh] Termos MeSH primário: Neoplasias da Mama/química
Sistemas de Liberação de Medicamentos/métodos
Epirubicina/farmacocinética
Nanopartículas/química
Silanos/farmacocinética
Dióxido de Silício/química
[Mh] Termos MeSH secundário: Neoplasias da Mama/metabolismo
Linhagem Celular Tumoral
Liberação Controlada de Fármacos
Epirubicina/química
Seres Humanos
Células MCF-7
Tamanho da Partícula
Silanos/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Silanes); 3Z8479ZZ5X (Epirubicin); 7631-86-9 (Silicon Dioxide); 8CB1M08OIW ((3-mercaptopropyl)trimethoxysilane)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180111
[Lr] Data última revisão:
180111
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170824
[St] Status:MEDLINE
[do] DOI:10.1080/03639045.2017.1371734


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[PMID]:28740040
[Au] Autor:Kawano Y; Otsu S; Bamba T; Hanawa T
[Ad] Endereço:Faculty of Pharmaceutical Sciences, Tokyo University of Science.
[Ti] Título:[Study of Interaction between Fluorinated Coating Glass and the Medicines].
[So] Source:Yakugaku Zasshi;137(11):1409-1417, 2017 Nov 01.
[Is] ISSN:1347-5231
[Cp] País de publicação:Japan
[La] Idioma:jpn
[Ab] Resumo:The adsorption of active pharmaceutical ingredients on the surface of medical devices such as polyvinl chloride, ethylene-vinyl acetate copolymer and glass often prevent the acuurate dose of drug. At dispensing of pharmaceuticals, mètre glass (MG) has been widely used for dispensing syrup. When measuring the viscous syrup, it often takes long time to dispense the accurate volume due to their adhesiveness on the glass surface. In this study, we investigate the adhesion of various syrups to MG made with uncoated glass or glass with a strongly hydrophobic silicone or fluorinated coating in terms of the following formulation parameters: viscosity, surface tension, contact angle, and surface free energy. The contact angles for syrups on the coated glass surfaces were significantly higher than those on the uncoated glass surface. In addition, the relationship between surface tension and contact angle was examined. We found that the contact angle was independent of surface tension for the uncoated glass, while it increased with increasing surface tension for the coated glasses. These results can be explained as follows: the silicone or fluorinated coatings inhibit the hydrogen bonding that usually takes place between water and silanol and siloxane groups at glass surfaces. The coatings reduced the surface free energy and increased the hydrophobicity of the glass, reduced its wettability by the syrups, and thus reduced the adhesion loss for the syrups. It was considered that as for the hydrophobic action, properties of matter of sample influence the coated device by coating in order that it is reinforced.
[Mh] Termos MeSH primário: Adesividade
Formas de Dosagem
Polímeros de Fluorcarboneto
Vidro
Interações Hidrofóbicas e Hidrofílicas
Propriedades de Superfície
[Mh] Termos MeSH secundário: Ligações de Hidrogênio
Silanos
Silicones
Siloxanas
Viscosidade
Água
Molhabilidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Dosage Forms); 0 (Fluorocarbon Polymers); 0 (Silanes); 0 (Silicones); 0 (Siloxanes); 059QF0KO0R (Water); 079V3J9O3X (silanol)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180103
[Lr] Data última revisão:
180103
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170726
[St] Status:MEDLINE
[do] DOI:10.1248/yakushi.17-00108


  8 / 5576 MEDLINE  
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[PMID]:28465106
[Au] Autor:Santini GC; Potrich C; Lunelli L; Vanzetti L; Marasso SL; Cocuzza M; Pirri FC; Pederzolli C
[Ad] Endereço:Fondazione Bruno Kessler, Laboratory of Biomolecular Sequence and Structure Analysis for Health, Via Sommarive 18, 38123, Povo, Trento, Italy.
[Ti] Título:miRNA purification with an optimized PDMS microdevice: Toward the direct purification of low abundant circulating biomarkers.
[So] Source:Biophys Chem;229:142-150, 2017 10.
[Is] ISSN:1873-4200
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:A reliable clinical assay based on circulating microRNAs (miRNAs) as biomarkers is highly required. Microdevices offer an attractive solution as a fast and inexpensive way of concentrating these biomarkers from a low sample volume. A previously developed polydimethylsiloxane (PDMS) microdevice able to purify and detect circulating miRNAs was here optimized. The optimization of the morphological and chemical surface properties by nanopatterning and functionalization is presented. Surfaces were firstly characterized by atomic force microscopy (AFM), X-ray photoelectron spectroscopy (XPS), fluorescamine assay and s-SDTB (sulphosuccinimidyl-4-o-(4,4-dimethoxytrityl) butyrate) assay and subsequently tested for their capacity to adsorb a fluorescent miRNA. From our analysis, modification of surface charge with 0.1% APTMS ((3-Aminopropyl)trimethoxysilane) and 0.9% PEG-s (2-[Methoxy-(polyethyleneoxy)propyl]trimethoxysilane) performed at 60°C for 10min was identified as the best purification condition. Our optimized microdevice integrated with real-time PCR detection, was demonstrated to selectively purify both synthetic and natural circulating miRNAs with a sensitivity of 0.01pM.
[Mh] Termos MeSH primário: Biomarcadores/sangue
Dimetilpolisiloxanos/química
MicroRNAs/isolamento & purificação
Técnicas Analíticas Microfluídicas/métodos
[Mh] Termos MeSH secundário: Corantes Fluorescentes/química
Seres Humanos
Isocianatos/química
MicroRNAs/sangue
MicroRNAs/química
Técnicas Analíticas Microfluídicas/instrumentação
Microscopia de Força Atômica
Espectroscopia Fotoeletrônica
Reação em Cadeia da Polimerase em Tempo Real
Silanos/química
Propriedades de Superfície
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (3-isocyanatopropyl trimethoxysilane); 0 (Biomarkers); 0 (Dimethylpolysiloxanes); 0 (Fluorescent Dyes); 0 (Isocyanates); 0 (MicroRNAs); 0 (Silanes); 63148-62-9 (baysilon)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171213
[Lr] Data última revisão:
171213
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170504
[St] Status:MEDLINE


  9 / 5576 MEDLINE  
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[PMID]:28985483
[Au] Autor:Brigante TAV; Miranda LFC; de Souza ID; Acquaro Junior VR; Queiroz MEC
[Ad] Endereço:School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, 14040-903 Ribeirão Preto, Brazil.
[Ti] Título:Pipette tip dummy molecularly imprinted solid-phase extraction of Bisphenol A from urine samples and analysis by gas chromatography coupled to mass spectrometry.
[So] Source:J Chromatogr B Analyt Technol Biomed Life Sci;1067:25-33, 2017 Nov 01.
[Is] ISSN:1873-376X
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Molecularly imprinted polymers (MIPs) were synthesized and used as sorbent for Bisphenol A (BPA) pipette tip solid-phase microextraction from urine samples and BPA analysis by gas chromatography coupled to mass spectrometry (GC-MS). The MIPs were synthesized by the sol-gel methodology. Aminopropyltriethoxysilane (APTES) and tetraethyl orthosilicate (TEOS) were used as functional monomer and cross-linking reagent, respectively. BPA and tetrabromobisphenol A (TBBPA) were evaluated as template during MIP synthesis. The BPA-based MIP displayed slightly higher extraction efficiency than the TBBPA-based dummy molecularly imprinted polymer (DMIP), but the TBBPA-based DMIP was selected as sorbent to minimize interference from leaked template. Comparison of the TBBPA-based DMIP, BPA-based MIP, and non-imprinted polymer (NIP) extraction efficiencies attested that the TBBPA-based DMIP was selective. The synthesized polymers were characterized by Scanning Electron Microscopy (SEM) and Fourier Transform Infrared spectroscopy (FTIR). The TBBPA-based DMIP was reused for over 100 times, which confirmed its robustness. The developed method was linear from 50 to 500ngmL . Precision values had coefficient of variation (CV) ranging from 4 to 14%. The accuracy relative standard deviation values (RSD) varied from -13.6 to 12.3%.
[Mh] Termos MeSH primário: Compostos Benzidrílicos/urina
Cromatografia Gasosa-Espectrometria de Massas/métodos
Impressão Molecular/métodos
Fenóis/urina
Extração em Fase Sólida/métodos
[Mh] Termos MeSH secundário: Seres Humanos
Limite de Detecção
Modelos Lineares
Bifenil Polibromatos/química
Propilaminas/química
Reprodutibilidade dos Testes
Silanos/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Benzhydryl Compounds); 0 (Phenols); 0 (Polybrominated Biphenyls); 0 (Propylamines); 0 (Silanes); FQI02RFC3A (tetrabromobisphenol A); L8S6UBW552 (amino-propyl-triethoxysilane); MLT3645I99 (bisphenol A)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171024
[Lr] Data última revisão:
171024
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171007
[St] Status:MEDLINE


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[PMID]:28721039
[Au] Autor:Bao Z; Sun J; Zhao X; Li Z; Cui S; Meng Q; Zhang Y; Wang T; Jiang Y
[Ad] Endereço:Department of Gastrointestinal Surgery, Lianyungang First People's Hospital, Affiliated Hospital of the Clinical Medical School of Nanjing Medical University, Lianyungang.
[Ti] Título:Top-down nanofabrication of silicon nanoribbon field effect transistor (Si-NR FET) for carcinoembryonic antigen detection.
[So] Source:Int J Nanomedicine;12:4623-4631, 2017.
[Is] ISSN:1178-2013
[Cp] País de publicação:New Zealand
[La] Idioma:eng
[Ab] Resumo:Sensitive and quantitative detection of tumor markers is highly required in the clinic for cancer diagnosis and consequent treatment. A field-effect transistor-based (FET-based) nanobiosensor emerges with characteristics of being label-free, real-time, having high sensitivity, and providing direct electrical readout for detection of biomarkers. In this paper, a top-down approach is proposed and implemented to fulfill a novel silicon nano-ribbon FET, which acts as biomarker sensor for future clinical application. Compared with the bottom-up approach, a top-down fabrication approach can confine width and length of the silicon FET precisely to control its electrical properties. The silicon nanoribbon (Si-NR) transistor is fabricated on a Silicon-on-Insulator (SOI) substrate by a top-down approach with complementary metal oxide semiconductor (CMOS)-compatible technology. After the preparation, the surface of Si-NR is functionalized with 3-aminopropyltriethoxysilane (APTES). Glutaraldehyde is utilized to bind the amino terminals of APTES and antibody on the surface. Finally, a microfluidic channel is integrated on the top of the device, acting as a flowing channel for the carcinoembryonic antigen (CEA) solution. The Si-NR FET is 120 nm in width and 25 nm in height, with ambipolar electrical characteristics. A logarithmic relationship between the changing ratio of the current and the CEA concentration is measured in the range of 0.1-100 ng/mL. The sensitivity of detection is measured as 10 pg/mL. The top-down fabricated biochip shows feasibility in direct detecting of CEA with the benefits of real-time, low cost, and high sensitivity as a promising biosensor for tumor early diagnosis.
[Mh] Termos MeSH primário: Técnicas Biossensoriais/instrumentação
Antígeno Carcinoembrionário/análise
Nanotecnologia/métodos
Nanotubos de Carbono/química
[Mh] Termos MeSH secundário: Biomarcadores Tumorais/análise
Desenho de Equipamento
Seres Humanos
Dispositivos Lab-On-A-Chip
Propilaminas/química
Sensibilidade e Especificidade
Silanos/química
Silício/química
Transistores Eletrônicos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biomarkers, Tumor); 0 (Carcinoembryonic Antigen); 0 (Nanotubes, Carbon); 0 (Propylamines); 0 (Silanes); L8S6UBW552 (amino-propyl-triethoxysilane); Z4152N8IUI (Silicon)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171010
[Lr] Data última revisão:
171010
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170720
[St] Status:MEDLINE
[do] DOI:10.2147/IJN.S135985



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