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[PMID]:29184999
[Au] Autor:Oya M; Suzuki H; Anas ARJ; Oishi K; Ono K; Yamaguchi S; Eguchi M; Sawada M
[Ad] Endereço:Department of Brain Function, Division of Stress Adaptation and Protection, Research Institute of Environmental Medicine, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, Aichi, 464-8601, Japan.
[Ti] Título:LC-MS/MS imaging with thermal film-based laser microdissection.
[So] Source:Anal Bioanal Chem;410(2):491-499, 2018 Jan.
[Is] ISSN:1618-2650
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Mass spectrometry (MS) imaging is a useful tool for direct and simultaneous visualization of specific molecules. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) is used to evaluate the abundance of molecules in tissues using sample homogenates. To date, however, LC-MS/MS has not been utilized as an imaging tool because spatial information is lost during sample preparation. Here we report a new approach for LC-MS/MS imaging using a thermal film-based laser microdissection (LMD) technique. To isolate tissue spots, our LMD system uses a 808-nm near infrared laser, the diameter of which can be freely changed from 2.7 to 500 µm; for imaging purposes in this study, the diameter was fixed at 40 µm, allowing acquisition of LC-MS/MS images at a 40-µm resolution. The isolated spots are arranged on a thermal film at 4.5-mm intervals, corresponding to the well spacing on a 384-well plate. Each tissue spot is handled on the film in such a manner as to maintain its spatial information, allowing it to be extracted separately in its individual well. Using analytical LC-MS/MS in combination with the spatial information of each sample, we can reconstruct LC-MS/MS images. With this imaging technique, we successfully obtained the distributions of pilocarpine, glutamate, γ-aminobutyric acid, acetylcholine, and choline in a cross-section of mouse hippocampus. The protocol we established in this study is applicable to revealing the neurochemistry of pilocarpine model of epilepsy. Our system has a wide range of uses in fields such as biology, pharmacology, pathology, and neuroscience. Graphical abstract Schematic Indication of LMD-LC-MS/MS imaging.
[Mh] Termos MeSH primário: Hipocampo/química
Microdissecção e Captura a Laser/métodos
Neurotransmissores/análise
Espectrometria de Massas em Tandem/métodos
[Mh] Termos MeSH secundário: Acetilcolina/análise
Animais
Colina/análise
Cromatografia Líquida/métodos
Epilepsia/diagnóstico
Epilepsia/patologia
Feminino
Ácido Glutâmico/análise
Hipocampo/patologia
Camundongos Endogâmicos C57BL
Pilocarpina/análise
Ácido gama-Aminobutírico/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Neurotransmitter Agents); 01MI4Q9DI3 (Pilocarpine); 3KX376GY7L (Glutamic Acid); 56-12-2 (gamma-Aminobutyric Acid); N91BDP6H0X (Choline); N9YNS0M02X (Acetylcholine)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171130
[St] Status:MEDLINE
[do] DOI:10.1007/s00216-017-0739-2


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[PMID]:27772535
[Au] Autor:Ljungberg M; Nilsson MKL; Melin K; Jönsson L; Carlsson A; Carlsson Å; Forssell-Aronsson E; Ivarsson T; Carlsson M; Starck G
[Ad] Endereço:1Department of Radiation Physics,Institute of Clinical Sciences,Sahlgrenska Academy,University of Gothenburg,Göteborg,Sweden.
[Ti] Título:1H magnetic resonance spectroscopy evidence for occipital involvement in treatment-naive paediatric obsessive-compulsive disorder.
[So] Source:Acta Neuropsychiatr;29(3):179-190, 2017 Jun.
[Is] ISSN:1601-5215
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:OBJECTIVE: Obsessive-compulsive disorder (OCD) is a chronic psychiatric disorder leading to considerable distress and disability. Therapies are effective in a majority of paediatric patients, however, many only get partial response. It is therefore important to study the underlying pathophysiology of the disorder. METHODS: 1H magnetic resonance spectroscopy (MRS) was used to study the concentration of brain metabolites in four different locations (cingulate gyrus and sulcus, occipital cortex, thalamus and right caudate nucleus). Treatment-naive children and adolescents with OCD (13 subjects) were compared with a group of healthy age- and gender-matched subjects (11 subjects). Multivariate analyses were performed on the concentration values. RESULTS: No separation between controls and patients was found. However, a correlation between metabolite concentrations and symptom severity as measured with the Children's Yale-Brown Obsessive-Compulsive Scale (CY-BOCS) was found. Strongest was the correlation with the CY-BOCS obsession subscore and aspartate and choline in the caudate nucleus (positively correlated with obsessions), lipids at 2 and 0.9 ppm in thalamus, and occipital glutamate+glutamine, N-acetylaspartate and myo-inosytol (negatively correlated with obsessions). CONCLUSIONS: The observed correlations between 1H MRS and CY-BOCS in treatment-naive patients further supports an occipital involvement in OCD. The results are consistent with our previous study on adult OCD patients. The 1H MRS data were not supportive of a separation between the patient and control groups.
[Mh] Termos MeSH primário: Encéfalo/diagnóstico por imagem
Transtorno Obsessivo-Compulsivo/diagnóstico por imagem
Transtorno Obsessivo-Compulsivo/metabolismo
Lobo Occipital/diagnóstico por imagem
Espectroscopia de Prótons por Ressonância Magnética/métodos
[Mh] Termos MeSH secundário: Adolescente
Ácido Aspártico/análogos & derivados
Ácido Aspártico/metabolismo
Encéfalo/metabolismo
Criança
Colina/metabolismo
Feminino
Seres Humanos
Inositol/metabolismo
Masculino
Transtorno Obsessivo-Compulsivo/tratamento farmacológico
Transtorno Obsessivo-Compulsivo/fisiopatologia
Lobo Occipital/metabolismo
Índice de Gravidade de Doença
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
30KYC7MIAI (Aspartic Acid); 4L6452S749 (Inositol); 997-55-7 (N-acetylaspartate); N91BDP6H0X (Choline)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180220
[Lr] Data última revisão:
180220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161025
[St] Status:MEDLINE
[do] DOI:10.1017/neu.2016.52


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[PMID]:29244873
[Au] Autor:Yoo W; Gjuka D; Stevenson HL; Song X; Shen H; Yoo SY; Wang J; Fallon M; Ioannou GN; Harrison SA; Beretta L
[Ad] Endereço:Department of Molecular and Cellular Oncology, The University of Texas MD Anderson Cancer Center, Houston, Texas, United States of America.
[Ti] Título:Fatty acids in non-alcoholic steatohepatitis: Focus on pentadecanoic acid.
[So] Source:PLoS One;12(12):e0189965, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Non-alcoholic fatty liver disease (NAFLD) is the most common form of liver disease and ranges from isolated steatosis to NASH. To determine whether circulating fatty acids could serve as diagnostic markers of NAFLD severity and whether specific fatty acids could contribute to the pathogenesis of NASH, we analyzed two independent NAFLD patient cohorts and used the methionine- and choline-deficient diet (MCD) NASH mouse model. We identified six fatty acids that could serve as non-invasive markers of NASH in patients with NAFLD. Serum levels of 15:0, 17:0 and 16:1n7t negatively correlated with NAFLD activity scores and hepatocyte ballooning scores, while 18:1n7c serum levels strongly correlated with fibrosis stage and liver inflammation. Serum levels of 15:0 and 17:0 also negatively correlated with fasting glucose and AST, while 16:1n7c and 18:1n7c levels positively correlated with AST and ferritin, respectively. Inclusion of demographic and clinical parameters improved the performance of the fatty acid panels in detecting NASH in NAFLD patients. The panel [15:0, 16:1n7t, 18:1n7c, 22:5n3, age, ferritin and APRI] predicted intermediate or advanced fibrosis in NAFLD patients, with 82% sensitivity at 90% specificity [AUROC = 0.92]. 15:0 and 18:1n7c were further selected for functional studies in vivo. Mice treated with 15:0-supplemented MCD diet showed reduced AST levels and hepatic infiltration of ceroid-laden macrophages compared to MCD-treated mice, suggesting that 15:0 deficiency contributes to liver injury in NASH. In contrast, 18:1n7c-supplemented MCD diet didn't affect liver pathology. In conclusion, 15:0 may serve as a promising biomarker or therapeutic target in NASH, opening avenues for the integration of diagnosis and treatment.
[Mh] Termos MeSH primário: Ácidos Graxos/metabolismo
Hepatócitos/metabolismo
Hepatopatia Gordurosa não Alcoólica/metabolismo
[Mh] Termos MeSH secundário: Animais
Colina/metabolismo
Deficiência de Colina/genética
Deficiência de Colina/metabolismo
Modelos Animais de Doenças
Ácidos Graxos/isolamento & purificação
Hepatócitos/patologia
Seres Humanos
Fígado/metabolismo
Fígado/patologia
Metionina/deficiência
Metionina/metabolismo
Camundongos
Camundongos Endogâmicos C57BL
Hepatopatia Gordurosa não Alcoólica/dietoterapia
Hepatopatia Gordurosa não Alcoólica/patologia
Triglicerídeos/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fatty Acids); 0 (Triglycerides); AE28F7PNPL (Methionine); CCW02D961F (pentadecanoic acid); N91BDP6H0X (Choline)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180102
[Lr] Data última revisão:
180102
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171216
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0189965


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[PMID]:27771079
[Au] Autor:de Veth MJ; Artegoitia VM; Campagna SR; Lapierre H; Harte F; Girard CL
[Ad] Endereço:BioNarus LLC, Cary, NC 27512; Department of Animal Science, University of Tennessee, Knoxville 37996. Electronic address: mdeveth@bionarus.com.
[Ti] Título:Choline absorption and evaluation of bioavailability markers when supplementing choline to lactating dairy cows.
[So] Source:J Dairy Sci;99(12):9732-9744, 2016 Dec.
[Is] ISSN:1525-3198
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The metabolites of choline have a central role in many mammalian biological processes, and choline supplementation to the periparturient dairy cow improves hepatic lipid metabolism. However, variability in responses to choline supplementation has highlighted a lack of understanding of choline absorption in the lactating dairy cow. Our objective was to determine net choline absorption by measuring net portal fluxes of choline and choline metabolites in cows receiving either dietary supplements of rumen-protected choline (RPC) or abomasal delivery of choline (ADC). We also evaluated markers for choline bioavailability by examining relationships between net portal absorption of choline and choline metabolites in plasma and milk. Five late-lactation Holstein cows were used in a 5×5 Latin square design, with 5-d treatment periods and a 2-d interval between periods. Treatments were (1) control (0g/d of choline), (2) 12.5g/d of choline fed as RPC, (3) 25g/d of choline fed as RPC, (4) 12.5g/d of choline provided as ADC, and (5) 25g/d of choline provided as ADC. At the end of each 5-d period, milk was sampled and 9 blood samples were collected simultaneously from an artery and portal vein at 30-min intervals. Plasma, milk, and feed ingredient concentrations of acetylcholine, betaine, free choline, glycerophosphocholine, lysophosphatidylcholine, phosphatidylcholine, phosphocholine, and sphingomyelin were quantified by hydrophilic interaction liquid chromatography-tandem mass spectrometry. With an increasing dose of ADC, the net portal flux of free choline increased and regression analysis indicated 61% net absorption of the infused dose. Among the choline metabolites, only concentrations of betaine, free choline, and phosphocholine increased in both arterial plasma (3.9, 1.9, and 0.4 times, respectively) and milk (2.5, 1.4, and 1.0 times, respectively) with 25g/d of ADC relative to the control. For RPC, the net portal flux of free choline was low relative to ADC (13%), which was similar to the relative difference observed in the concentrations and yields of milk free choline and betaine (averaged 21%). When evaluating markers for choline bioavailability, betaine was the leading candidate. Betaine in plasma and milk (alone or in combination with phosphocholine) was strongly associated with net free choline portal flux (coefficient of determination ranging from 0.64 to 0.79). In summary, free choline supply to the lactating dairy cow increases only specific choline metabolites in plasma and milk, which can be potential markers for choline bioavailability.
[Mh] Termos MeSH primário: Colina/administração & dosagem
Lactação
[Mh] Termos MeSH secundário: Animais
Disponibilidade Biológica
Bovinos
Dieta/veterinária
Suplementos Nutricionais
Feminino
Leite/química
Rúmen/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
N91BDP6H0X (Choline)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:171204
[Lr] Data última revisão:
171204
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161025
[St] Status:MEDLINE


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[PMID]:28454818
[Au] Autor:Shiu YL; Chiu KH; Huynh TG; Liu PC; Liu CH
[Ad] Endereço:Department of Aquaculture, National Pingtung University of Science and Technology, Pingtung, Taiwan.
[Ti] Título:Plasma immune protein analysis in the orange-spotted grouper Epinephelus coioides: Evidence for altered expressions of immune factors associated with a choline-supplemented diet.
[So] Source:Fish Shellfish Immunol;65:235-243, 2017 Jun.
[Is] ISSN:1095-9947
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:This study aimed to unravel the regulatory roles of choline in activating immune responses and disease resistance of the orange-spotted grouper Epinephelus coioides. Fish were fed a choline-supplemented diet at 1 g kg of feed for 30 days. Fish fed a fish meal basal diet without choline-supplement served as controls. At the end of the feeding trial, fish were challenged with Vibrio alginolyticus. Meanwhile, plasma proteomics of fish in each group were also evaluated by two-dimensional gel electrophoresis (2-DE), and differentially expressed proteins were identified by tandem mass spectrophotometry (MS/MS), then a Western blot analysis or real-time polymerase chain reaction was used to confirm differential expressions of immune-enhancing proteins. Results showed that choline significantly increased survival of E. coioides 48 days after being injected with V. alginolyticus. From maps of plasma proteins, a comparative analysis between the control and choline groups revealed that 111 spots matched, with 26 altered expression spots in the choline group. Of these 26 spots, 16 were upregulated and 10 downregulated. After protein identification by reverse-phase nano-high-performance liquid chromatography-electrospray ionization MS/MS analysis, eight of 26 proteins were found to be immune-related proteins, all of which were upregulated, including complement 3 (C3), alpha-2-macroglobulin-P-like isoform (A2M), fibrinogen beta chain precursor (FBG), and immunoglobulin heavy constant mu (Ighm) proteins. Expression of the A2M protein and A2M enzyme activity in plasma of fish fed choline significantly increased compared to the control group. Additionally, A2M messenger (m)RNA transcripts were also upregulated in the liver and kidneys. Significantly higher C3 expressions at both the mRNA and protein levels were detected in the liver of fish in the choline group. Moreover, FBG gene expressions in the liver and kidneys significantly increased, while Ighm increased in the kidneys and spleen of fish in the choline group. Our results suggest that dietary administration of choline can protect grouper against bacterial infections through activating the complement system, thereby inducing antiprotease activity and natural antibodies that play important roles in the innate immune system of fish.
[Mh] Termos MeSH primário: Bass
Colina
Dieta/veterinária
Suplementos Nutricionais
Resistência à Doença
Imunidade Inata/fisiologia
Imunomodulação/fisiologia
[Mh] Termos MeSH secundário: Adjuvantes Imunológicos
Ração Animal/análise
Animais
Proteínas Sanguíneas/genética
Proteínas Sanguíneas/metabolismo
Doenças dos Peixes/genética
Doenças dos Peixes/imunologia
Doenças dos Peixes/microbiologia
Proteínas de Peixes/genética
Proteínas de Peixes/metabolismo
Especificidade de Órgãos
Vibrioses/imunologia
Vibrioses/microbiologia
Vibrioses/veterinária
Vibrio alginolyticus/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Adjuvants, Immunologic); 0 (Blood Proteins); 0 (Fish Proteins); N91BDP6H0X (Choline)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171128
[Lr] Data última revisão:
171128
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170430
[St] Status:MEDLINE


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[PMID]:28980836
[Au] Autor:Franzese C; Lopci E; Di Brina L; D'Agostino GR; Navarria P; Mancosu P; Tomatis S; Chiti A; Scorsetti M
[Ad] Endereço:a Radiotherapy and Radiosurgery , Humanitas Clinical and Research Center , Via Manzoni Rozzano ( Milano ) - Italy.
[Ti] Título:11C-Choline-Pet Guided Stereotactic Body Radiation Therapy for Lymph Node Metastases in Oligometastatic Prostate Cancer.
[So] Source:Cancer Invest;35(9):586-593, 2017 Oct 21.
[Is] ISSN:1532-4192
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:INTRODUCTION: aim is outcome of 11C-Choline-PET guided SBRT on lymph node metastases. MATERIALS AND METHODS: patients with 1 - 4 lymph node metastases detected by 11C-choline-PET were treated with SBRT. Toxicity, treated metastases control and Progression Free Survival were computed. RESULTS: twenty-six patients, 38 lymph node metastases were irradiated. No grade ≥ 2 toxicity. Median PSA-nadir after RT was 1.02 ng/mL. Post-treatment 11C-Choline-PET showed metabolic complete response in 17 metastases (44,7%), partial response in 9 metastases (38%). CONCLUSION: SBRT is effective and safe for lymph node metastases. PET is important in identification of gross tumor and evaluation of the response.
[Mh] Termos MeSH primário: Radioisótopos de Carbono/administração & dosagem
Colina/administração & dosagem
Linfonodos/cirurgia
Tomografia Computadorizada com Tomografia por Emissão de Pósitrons
Neoplasias da Próstata/cirurgia
Compostos Radiofarmacêuticos/administração & dosagem
Radiocirurgia/métodos
[Mh] Termos MeSH secundário: Idoso
Intervalo Livre de Doença
Seres Humanos
Calicreínas/sangue
Estimativa de Kaplan-Meier
Linfonodos/diagnóstico por imagem
Linfonodos/patologia
Metástase Linfática
Masculino
Valor Preditivo dos Testes
Antígeno Prostático Específico/sangue
Neoplasias da Próstata/sangue
Neoplasias da Próstata/diagnóstico por imagem
Neoplasias da Próstata/patologia
Radiocirurgia/efeitos adversos
Estudos Retrospectivos
Fatores de Tempo
Resultado do Tratamento
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Carbon Radioisotopes); 0 (Radiopharmaceuticals); EC 3.4.21.- (Kallikreins); EC 3.4.21.- (kallikrein-related peptidase 3, human); EC 3.4.21.77 (Prostate-Specific Antigen); N91BDP6H0X (Choline)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171113
[Lr] Data última revisão:
171113
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171006
[St] Status:MEDLINE
[do] DOI:10.1080/07357907.2017.1375116


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[PMID]:28895242
[Au] Autor:Ikawa-Yoshida A; Matsuo S; Kato A; Ohmori Y; Higashida A; Kaneko E; Matsumoto M
[Ad] Endereço:Chugai Research Institute for Medical Science, Inc., Gotemba, Japan.
[Ti] Título:Hepatocellular carcinoma in a mouse model fed a choline-deficient, L-amino acid-defined, high-fat diet.
[So] Source:Int J Exp Pathol;98(4):221-233, 2017 Aug.
[Is] ISSN:1365-2613
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Hepatocellular carcinoma (HCC) is a common cancer worldwide and represents the outcome of the natural history of chronic liver disease. The growing rates of HCC may be partially attributable to increased numbers of people with non-alcoholic fatty liver disease (NAFLD) and non-alcoholic steatohepatitis (NASH). However, details of the liver-specific molecular mechanisms responsible for the NAFLD-NASH-HCC progression remain unclear, and mouse models that can be used to explore the exact factors that influence the progression of NAFLD/NASH to the more chronic stages of liver disease and subsequent HCC are not yet fully established. We have previously reported a choline-deficient, L-amino acid-defined, high-fat diet (CDAHFD) as a dietary NASH model with rapidly progressive liver fibrosis in mice. The current study in C57BL/6J mice fed CDAHFD provided evidence for the chronic persistence of advanced hepatic fibrosis in NASH and disease progression towards HCC in a period of 36 weeks. When mice fed CDAHFD were switched back to a standard diet, hepatic steatosis was normalized and NAFLD activity score improved, but HCC incidence increased and the phenotype of fibrosis-associated HCC development was observed. Moreover, when mice continued to be fed CDAHFD for 60 weeks, HCC further developed without severe body weight loss or carcinogenesis in other organs. The autochthonous tumours showed a variety of histological features and architectural patterns including trabecular, pseudoglandular and solid growth. The CDAHFD mouse model might be a useful tool for studying the development of HCC from NAFLD/NASH, and potentially useful for better understanding pathological changes during hepatocarcinogenesis.
[Mh] Termos MeSH primário: Carcinoma Hepatocelular
Transformação Celular Neoplásica
Deficiência de Colina/metabolismo
Dieta Hiperlipídica
Neoplasias Hepáticas/patologia
Hepatopatia Gordurosa não Alcoólica/patologia
[Mh] Termos MeSH secundário: Animais
Colina/metabolismo
Colina/farmacologia
Modelos Animais de Doenças
Masculino
Camundongos Endogâmicos C57BL
Hepatopatia Gordurosa não Alcoólica/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
N91BDP6H0X (Choline)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171019
[Lr] Data última revisão:
171019
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170913
[St] Status:MEDLINE
[do] DOI:10.1111/iep.12240


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[PMID]:28873429
[Au] Autor:Pierce AA; Duwaerts CC; Siao K; Mattis AN; Goodsell A; Baron JL; Maher JJ
[Ad] Endereço:Department of Medicine, University of California, San Francisco, San Francisco, California, United States of America.
[Ti] Título:CD18 deficiency improves liver injury in the MCD model of steatohepatitis.
[So] Source:PLoS One;12(9):e0183912, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Neutrophils and macrophages are important constituents of the hepatic inflammatory infiltrate in non-alcoholic steatohepatitis. These innate immune cells express CD18, an adhesion molecule that facilitates leukocyte activation. In the context of fatty liver, activation of infiltrated leukocytes is believed to enhance hepatocellular injury. The objective of this study was to determine the degree to which activated innate immune cells promote steatohepatitis by comparing hepatic outcomes in wild-type and CD18-mutant mice fed a methionine-choline-deficient (MCD) diet. After 3 weeks of MCD feeding, hepatocyte injury, based on serum ALT elevation, was 40% lower in CD18-mutant than wild-type mice. Leukocyte infiltration into the liver was not impaired in CD18-mutant mice, but leukocyte activation was markedly reduced, as shown by the lack of evidence of oxidant production. Despite having reduced hepatocellular injury, CD18-mutant mice developed significantly more hepatic steatosis than wild-type mice after MCD feeding. This coincided with greater hepatic induction of pro-inflammatory and lipogenic genes as well as a modest reduction in hepatic expression of adipose triglyceride lipase. Overall, the data indicate that CD18 deficiency curbs MCD-mediated liver injury by limiting the activation of innate immune cells in the liver without compromising intrahepatic cytokine activation. Reduced liver injury occurs at the expense of increased hepatic steatosis, which suggests that in addition to damaging hepatocytes, infiltrating leukocytes may influence lipid homeostasis in the liver.
[Mh] Termos MeSH primário: Antígenos CD18/genética
Antígenos CD18/fisiologia
Fígado Gorduroso/metabolismo
[Mh] Termos MeSH secundário: Tecido Adiposo/metabolismo
Animais
Colina/química
Citocinas/metabolismo
Modelos Animais de Doenças
Hepatócitos/citologia
Imunidade Inata
Inflamação
Leucócitos/citologia
Leucócitos/metabolismo
Lipase/metabolismo
Fígado/metabolismo
Masculino
Metionina/química
Camundongos
Camundongos Endogâmicos C57BL
Mutação
Oxigênio/química
Peroxidase/metabolismo
Triglicerídeos/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (CD18 Antigens); 0 (Cytokines); 0 (Triglycerides); AE28F7PNPL (Methionine); EC 1.11.1.7 (Peroxidase); EC 3.1.1.3 (Lipase); N91BDP6H0X (Choline); S88TT14065 (Oxygen)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170906
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0183912


  9 / 15086 MEDLINE  
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[PMID]:28847084
[Au] Autor:Yiin CL; Quitain AT; Yusup S; Uemura Y; Sasaki M; Kida T
[Ad] Endereço:Biomass Processing Cluster, Center of Biofuel and Biochemical Research, Chemical Engineering Department, Mission Oriented Research (Green Technology), Universiti Teknologi PETRONAS, Bandar Seri Iskandar, 32610 Tronoh, Perak, Malaysia.
[Ti] Título:Choline chloride (ChCl) and monosodium glutamate (MSG)-based green solvents from optimized cactus malic acid for biomass delignification.
[So] Source:Bioresour Technol;244(Pt 1):941-948, 2017 Nov.
[Is] ISSN:1873-2976
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:This work aimed to develop an efficient microwave-hydrothermal (MH) extraction of malic acid from abundant natural cactus as hydrogen bond donor (HBD) whereby the concentration was optimized using response surface methodology. The ideal process conditions were found to be at a solvent-to-feed ratio of 0.008, 120°C and 20min with 1.0g of oxidant, H O . Next generation environment-friendly solvents, low transition temperature mixtures (LTTMs) were synthesized from cactus malic acid with choline chloride (ChCl) and monosodium glutamate (MSG) as hydrogen bond acceptors (HBAs). The hydrogen-bonding interactions between the starting materials were determined. The efficiency of the LTTMs in removing lignin from oil palm biomass residues, empty fruit bunch (EFB) was also evaluated. The removal of amorphous hemicellulose and lignin after the pretreatment process resulted in an enhanced digestibility and thermal degradability of biomass.
[Mh] Termos MeSH primário: Cactaceae
Malatos
Glutamato de Sódio
[Mh] Termos MeSH secundário: Biomassa
Colina
Peróxido de Hidrogênio
Hidrólise
Solventes
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Malates); 0 (Solvents); 817L1N4CKP (malic acid); BBX060AN9V (Hydrogen Peroxide); N91BDP6H0X (Choline); W81N5U6R6U (Sodium Glutamate)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171023
[Lr] Data última revisão:
171023
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170830
[St] Status:MEDLINE


  10 / 15086 MEDLINE  
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[PMID]:28846866
[Au] Autor:Chen J; Wang Q; Liu M; Zhang L
[Ad] Endereço:Institute of Molecule Science, Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Shanxi University, Taiyuan 030006, PR China; Modern Research Center for Traditional Chinese Medicine, Shanxi University, Taiyuan 030006, Shanxi, PR China.
[Ti] Título:The effect of deep eutectic solvent on the pharmacokinetics of salvianolic acid B in rats and its acute toxicity test.
[So] Source:J Chromatogr B Analyt Technol Biomed Life Sci;1063:60-66, 2017 Sep 15.
[Is] ISSN:1873-376X
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Deep eutectic solvent (DES), the benign green solvent with uniquely physical properties, has been widely applied in various fields. Our previous study indicated that DES could improve the stability and extraction efficiency of salvianolic acid B (SAB). In this work, with SAB as a model drug, the feasibility of DES as a drug carrier for oral preparation was investigated by evaluating the influence of DES on the pharmacokinetics of SAB and the toxicity of DES. Acute oral toxicity test illustrated that choline chloride-glycerol (ChCl-GL, molar ratio 1:2) was non-toxic with the median lethal dose of 7733mg/kg. To comparison the difference of pharmacokinetics between SAB dissolved in ChCl-GL (1:2) and in water, a rapid and sensitive ultra-performance liquid chromatography coupled with mass spectrum was established to determine SAB and its metabolites in rat plasma. The method validation was also tested for the specificity, linearity (r >0.9980 over two orders of magnitude), precision (intra-day relative standard deviation (RSD)<2.73% and inter-day RSD<7.72%), extraction recovery (70.96-80.78%) and stability under three different situations. Compared to water, the pharmacokinetic parameters clarified that ChCl-GL (1:2) could promote the absorption of SAB, the peak concentration (C ) of 0.308±0.020mg/L was slightly higher than 0.277±0.024mg/L (SAB dissolved in water), and the peak time (T ) was significantly decreased from 30min (SAB dissolved in water) to 20min. There was no significant difference on the metabolites between SAB dissolved in ChCl-GL (1:2) and in water. This is the first report on the pharmacokinetic study of DES as a candidate of drug carrier, and the results provide a meaningful basis for the application of DES in pharmaceutical preparation.
[Mh] Termos MeSH primário: Benzofuranos/sangue
Benzofuranos/farmacocinética
Interações Ervas-Drogas
Solventes/farmacocinética
[Mh] Termos MeSH secundário: Animais
Comportamento Animal/efeitos dos fármacos
Benzofuranos/química
Colina/química
Colina/farmacocinética
Colina/toxicidade
Cromatografia Líquida de Alta Pressão/métodos
Feminino
Glicerol/química
Glicerol/farmacocinética
Glicerol/toxicidade
Limite de Detecção
Modelos Lineares
Masculino
Espectrometria de Massas/métodos
Camundongos
Ratos
Ratos Sprague-Dawley
Reprodutibilidade dos Testes
Solventes/química
Solventes/toxicidade
Água
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Benzofurans); 0 (Solvents); 059QF0KO0R (Water); C1GQ844199 (salvianolic acid B); N91BDP6H0X (Choline); PDC6A3C0OX (Glycerol)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171002
[Lr] Data última revisão:
171002
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170829
[St] Status:MEDLINE



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