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[PMID]:25182481
[Au] Autor:Zheng D; Shuai X; Li Y; Zhou P; Gong T; Sun X; Zhang Z
[Ad] Endereço:a Key Laboratory of Drug Targeting and Drug Delivery Systems, Ministry of Education, West China School of Pharmacy , Sichuan University , Chengdu , Sichuan , People's Republic of China and.
[Ti] Título:Novel flurbiprofen derivatives with improved brain delivery: synthesis, in vitro and in vivo evaluations.
[So] Source:Drug Deliv;23(7):2183-2192, 2016 Sep.
[Is] ISSN:1521-0464
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Tarenflurbil (R-flurbiprofen) was acknowledged as a promising candidate in Alzheimer's disease (AD) therapy. However, the Phase III study of tarenflurbil was extremely restricted by its poor delivery efficiency to the brain. To tackle this problem, the novel carriers for tarenflurbil, racemic flurbiprofen (FLU) derivatives (FLU-D1 and FLU-D2) modified by N,N-dimethylethanolamine-related structures were synthesized and characterized. These derivatives showed good safety level in vitro and they possessed much higher cellular uptake efficiency in brain endothelial cells than FLU did. More importantly, the uptake experiments suggested that they were internalized via active transport mechanisms. Biodistribution studies in rats also illustrated a remarkably enhanced accumulation of these derivatives in the brain. FLU-D2, the ester linkage form of these derivatives, achieved a higher brain-targeting efficiency. Its C and AUC were enhanced by 12.09-fold and 4.61-fold, respectively compared with those of FLU. Additionally, it could be hydrolyzed by esterase in the brain to release the parent FLU, which might facilitate its therapeutic effect. These in vitro and in vivo results highlighted the improvement of the brain-targeted delivery of FLU by making use of N,N-dimethylethanolamine ligand, with which an active transport mechanism was involved.
[Mh] Termos MeSH primário: Encéfalo/metabolismo
Flurbiprofeno/administração & dosagem
Flurbiprofeno/metabolismo
[Mh] Termos MeSH secundário: Animais
Linhagem Celular
Deanol/química
Portadores de Fármacos/química
Células Endoteliais/metabolismo
Flurbiprofeno/química
Masculino
Camundongos
Ratos
Ratos Sprague-Dawley
Distribuição Tecidual/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Drug Carriers); 2N6K9DRA24 (Deanol); 501W00OOWA (tarenflurbil); 5GRO578KLP (Flurbiprofen)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170302
[Lr] Data última revisão:
170302
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140904
[St] Status:MEDLINE


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[PMID]:26355619
[Au] Autor:Saxena SJ; Duque D; Schirripa MJ
[Ti] Título:Assessment of a Comprehensive Anti-Aging Neck Cream.
[So] Source:J Drugs Dermatol;14(9):997-1002, 2015 Sep.
[Is] ISSN:1545-9616
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:INTRODUCTION: With many effective anti-aging solutions for the face, consumer focus is now turning to other parts of the body including the delicate skin on the neck. This study investigates the effect of a new neck cream on the appearance of texture, fine lines and wrinkles, laxity, and hydration. METHODS: 85 adult females ages 35-65 with Fitzpatrick skin types I through IV applied the test neck cream twice daily for a 3-month study period. Screening was conducted at Baseline, 2, 30, 60, and 90 days via a virtual trial. Subjects rated satisfaction in each of 4 anti-aging categories including hydration, texture, appearance of wrinkles, and appearance of laxity as well as three product attributes including application, feel, and smell. RESULTS: Improvement was statistically significant for all measured categories (hydration, texture, appearance of wrinkles, and appearance of laxity) with 94% of study subjects noting improvement in one or more of the measured categories. Further, the quantity of "Satisfied" and "Highly Satisfied" assessments increased 8-fold from baseline with a 94x increase in the quantity of "Highly Satisfied" assessments. DISCUSSION: The results demonstrate the product's rapid and continuing ability to improve the self-perceived signs of aging in the neck area including improvement in skin texture on the neck and a reduction in the appearance of wrinkles and laxity along the jawline. Future studies are recommended to determine the primary action mechanisms and to assess the degree of improvement by blinded physician assessment.
[Mh] Termos MeSH primário: Envelhecimento da Pele/efeitos dos fármacos
Creme para a Pele/uso terapêutico
Fenômenos Fisiológicos da Pele/efeitos dos fármacos
[Mh] Termos MeSH secundário: Adulto
Idoso
Antioxidantes/uso terapêutico
Biotina/uso terapêutico
Deanol/uso terapêutico
Fármacos Dermatológicos/uso terapêutico
Combinação de Medicamentos
Feminino
Seres Humanos
Ácido Hialurônico/uso terapêutico
Higroscópicos/uso terapêutico
Meia-Idade
Pescoço
Satisfação do Paciente
Sesquiterpenos/uso terapêutico
Envelhecimento da Pele/patologia
Envelhecimento da Pele/fisiologia
Complexo Vitamínico B/uso terapêutico
Vitamina E/uso terapêutico
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antioxidants); 0 (Dermatologic Agents); 0 (Drug Combinations); 0 (Hygroscopic Agents); 0 (Sesquiterpenes); 12001-76-2 (Vitamin B Complex); 1406-18-4 (Vitamin E); 24WE03BX2T (bisabolol); 2N6K9DRA24 (Deanol); 6SO6U10H04 (Biotin); 9004-61-9 (Hyaluronic Acid)
[Em] Mês de entrada:1606
[Cu] Atualização por classe:150911
[Lr] Data última revisão:
150911
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150911
[St] Status:MEDLINE


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[PMID]:25633216
[Au] Autor:Pittenauer E; Rehulka P; Winkler W; Allmaier G
[Ad] Endereço:Institute of Chemical Technologies and Analytics, Vienna University of Technology, Getreidemarkt 9, 1060, Vienna, Austria.
[Ti] Título:Collision-induced dissociation of aminophospholipids (PE, MMPE, DMPE, PS): an apparently known fragmentation process revisited.
[So] Source:Anal Bioanal Chem;407(17):5079-89, 2015 Jul.
[Is] ISSN:1618-2650
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:A new type of low-mass substituted 4-oxazolin product ions of [M + H](+) precursor ions of aminophospholipids (glycerophosphatidylethanolamine, glycerophosphatidyl-N-methylethanolamine, glycerophosphatidyl-N,N-dimethylethanolamine, glycerophosphatidylserine) resulting from high-energy collision-induced dissociation (matrix-assisted laser desorption/ionization time-of-flight/reflectron time-of-flight mass spectrometry) and low-energy collision-induced dissociation (e.g., electrospray ionization quadrupole reflectron time-of-flight mass spectrometry) with accurate mass determination is described; these were previously misidentified as CHO-containing radical cationic product ions. The mechanism for the formation of these ions is proposed to be via rapid loss of water followed by cyclization to an 11-membered-ring transition state for the sn-1 fatty acid substituent and to a ten-membered-ring transition state for the sn-2 fatty acid substituent, and via final loss of monoacylglycerol phosphate, leading to substituted 4-oxazolin product ions. The minimum structural requirement for this interesting skeletal rearrangement fragmentation is an amino group linked to at least one hydrogen atom (i.e., ethanolamine, N-methylethanolamine, serine). Therefore, N,N-dimethylethanolamine derivates do not exhibit this type of fragmentation. The analytical value of these product ions is given by the fact that by post source decay and particularly high-energy collision-induced dissociation achieved via matrix-assisted laser desorption/ionization time-of-flight/reflectron time-of-flight mass spectrometry, the sn-2-related substituted 4-oxazolin product ion is always significantly more abundant than the sn-1-related one, which is quite helpful for detailed structural analysis of complex lipids. All other important product ions found are described in detail (following our previously published glycerophospholipid product ion nomenclature; Pittenauer and Allmaier, Int. J. Mass. Spectrom. 301:90-1012, 2011).
[Mh] Termos MeSH primário: Deanol/química
Etanolaminas/química
Oxazóis/química
Fosfatidiletanolaminas/química
Fosfatidilserinas/química
[Mh] Termos MeSH secundário: Íons/química
Espectrometria de Massas por Ionização por Electrospray
Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Ethanolamines); 0 (Ions); 0 (Oxazoles); 0 (Phosphatidylethanolamines); 0 (Phosphatidylserines); 2N6K9DRA24 (Deanol); ZMQ4G4V497 (N-methylaminoethanol)
[Em] Mês de entrada:1603
[Cu] Atualização por classe:160512
[Lr] Data última revisão:
160512
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150131
[St] Status:MEDLINE
[do] DOI:10.1007/s00216-015-8470-3


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[PMID]:25528441
[Au] Autor:Shahrisa A; Teimuri-Mofrad R; Gholamhosseini-Nazari M
[Ad] Endereço:Department of Organic and Biochemistry, Faculty of Chemistry, University of Tabriz, 5166614766, Tabriz, Iran, ashahrisa@yahoo.com.
[Ti] Título:Synthesis of a new class of Betti bases by the Mannich-type reaction: efficient, facile, solvent-free and one-pot protocol.
[So] Source:Mol Divers;19(1):87-101, 2015 Feb.
[Is] ISSN:1573-501X
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:A variety of organocatalysts has been screened for the synthesis of arylaminonaphthols. It has been shown that (N,N-dimethylethanolamine) is a highly efficient organocatalyst for the direct synthesis of a novel class of arylaminonaphthols via three-component condensation of 2-naphthol, aldehydes, and arylamines under solvent-free conditions. Mild, one-pot, and green reaction conditions, relatively short reaction times and good yields make this protocol highly significant. 25 new compounds have been synthesized by this method.
[Mh] Termos MeSH primário: Aldeídos/química
Aminas/química
Deanol/química
Naftóis/química
Naftóis/síntese química
[Mh] Termos MeSH secundário: Técnicas de Química Sintética
Modelos Moleculares
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Aldehydes); 0 (Amines); 0 (Naphthols); 2N6K9DRA24 (Deanol)
[Em] Mês de entrada:1509
[Cu] Atualização por classe:171121
[Lr] Data última revisão:
171121
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:141222
[St] Status:MEDLINE
[do] DOI:10.1007/s11030-014-9559-x


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[PMID]:25133239
[Au] Autor:Liu S; Chen Z; Cai X; Sun Y; Zhao C; Liu F; Liu D
[Ad] Endereço:Department of Plastic Surgery, Zhujiang Hospital, Southern Medical University, 253 GongYe Middle Avenue, Guangzhou, Guangdong 510280, China ; Department of Plastic and Aesthetic Surgery, The Affiliated Hospital of Medical School, Qingdao University, Qingdao, Shandong 266003, China.
[Ti] Título:Effects of dimethylaminoethanol and compound amino acid on D-galactose induced skin aging model of rat.
[So] Source:ScientificWorldJournal;2014:507351, 2014.
[Is] ISSN:1537-744X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:A lasting dream of human beings is to reverse or postpone aging. In this study, dimethylaminoethanol (DMAE) and compound amino acid (AA) in Mesotherapy were investigated for their potential antiaging effects on D-galactose induced aging skin. At 18 days after D-gal induction, each rat was treated with intradermal microinjection of saline, AA, 0.1% DMAE, 0.2% DMAE, 0.1% DMAE + AA, or 0.2% DMAE + AA, respectively. At 42 days after treatment, the skin wound was harvested and assayed. Measurement of epidermal and dermal thickness in 0.1% DMAE + AA and 0.2% DMAE + AA groups appeared significantly thicker than aging control rats. No differences were found in tissue water content among groups. Hydroxyproline in 0.1% DMAE + AA, 0.2% DMAE + AA, and sham control groups was much higher than all other groups. Collagen type I, type III, and MMP-1 expression was highly upregulated in both 0.1% DMAE + AA and 0.2% DMAE + AA groups compared with aging control. In contrast, TIMP-1 expression levels of various aging groups were significantly reduced when compared to sham control. Coinjection of DMAE and AA into target tissue has marked antiaging effects on D-galactose induced skin aging model of rat.
[Mh] Termos MeSH primário: Aminoácidos/farmacologia
Deanol/farmacologia
Envelhecimento da Pele/efeitos dos fármacos
Pele/efeitos dos fármacos
[Mh] Termos MeSH secundário: Animais
Colágeno/genética
Colágeno/metabolismo
Galactose/farmacologia
Masculino
Metaloproteinase 1 da Matriz/genética
Metaloproteinase 1 da Matriz/metabolismo
Ratos
Ratos Wistar
Pele/metabolismo
Inibidor Tecidual de Metaloproteinase-1/genética
Inibidor Tecidual de Metaloproteinase-1/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Amino Acids); 0 (Tissue Inhibitor of Metalloproteinase-1); 2N6K9DRA24 (Deanol); 9007-34-5 (Collagen); EC 3.4.24.7 (Matrix Metalloproteinase 1); X2RN3Q8DNE (Galactose)
[Em] Mês de entrada:1505
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140819
[St] Status:MEDLINE
[do] DOI:10.1155/2014/507351


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[PMID]:24614846
[Au] Autor:L'Haridon S; Chalopin M; Colombo D; Toffin L
[Ad] Endereço:Université de Bretagne Occidentale (UBO, UEB), Institut Universitaire Européen de la Mer (IUEM) - UMR 6197, Laboratoire de Microbiologie des Environnements Extrêmes (LMEE), Place Nicolas Copernic, F-29280 Plouzané, France CNRS, IUEM - UMR 6197, Laboratoire de Microbiologie des Environnements Extrême
[Ti] Título:Methanococcoides vulcani sp. nov., a marine methylotrophic methanogen that uses betaine, choline and N,N-dimethylethanolamine for methanogenesis, isolated from a mud volcano, and emended description of the genus Methanococcoides.
[So] Source:Int J Syst Evol Microbiol;64(Pt 6):1978-83, 2014 Jun.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A novel, strictly anaerobic, methylotrophic marine methanogen, strain SLH33(T), was isolated from deep sediment samples covered by an orange microbial mat collected from the Napoli Mud Volcano. Cells of strain SLH33(T) were Gram-stain-negative, motile, irregular cocci that occurred singly. Cells utilized trimethylamine, dimethylamine, monomethylamine, methanol, betaine, N,N-dimethylethanolamine and choline (N,N,N-trimethylethanolamine) as substrates for growth and methanogenesis. The optimal growth temperature was 30 °C; maximum growth rate was obtained at pH 7.0 in the presence of 0.5 M Na(+). The DNA G+C content of strain SLH33(T) was 43.4 mol%. Phylogenetic analyses based on 16S rRNA gene sequences placed strain SLH33(T) within the genus Methanococcoides. The novel isolate was related most closely to Methanococcoides methylutens TMA-10(T) (98.8% 16S rRNA gene sequence similarity) but distantly related to Methanococcoides burtonii DSM 6242(T) (97.6%) and Methanococcoides alaskense AK-5(T) (97.6%). DNA-DNA hybridization studies indicated that strain SLH33(T) represents a novel species, given that it shared less than 16% DNA-DNA relatedness with Methanococcoides methylutens TMA-10(T). The name Methanococcoides vulcani sp. nov. is proposed for this novel species, with strain SLH33(T) ( = DSM 26966(T) = JCM 19278(T)) as the type strain. An emended description of the genus Methanococcoides is also proposed.
[Mh] Termos MeSH primário: Fontes Hidrotermais/microbiologia
Methanosarcinaceae/classificação
Filogenia
[Mh] Termos MeSH secundário: Composição de Bases
Betaína/metabolismo
Colina/metabolismo
DNA Bacteriano/genética
Deanol/metabolismo
Mar Mediterrâneo
Methanosarcinaceae/genética
Methanosarcinaceae/isolamento & purificação
Dados de Sequência Molecular
Hibridização de Ácido Nucleico
Pigmentação
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (RNA, Ribosomal, 16S); 2N6K9DRA24 (Deanol); 3SCV180C9W (Betaine); N91BDP6H0X (Choline)
[Em] Mês de entrada:1407
[Cu] Atualização por classe:140610
[Lr] Data última revisão:
140610
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140312
[St] Status:MEDLINE
[do] DOI:10.1099/ijs.0.058289-0


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[PMID]:24429957
[Au] Autor:Noskov DS; Poroikov VV; Shikh EV; Iasnetsov VV
[Ti] Título:[Deanol aceglumate (nooclerin): clinical/pharmacological aspects and relevance in clinical practice].
[So] Source:Zh Nevrol Psikhiatr Im S S Korsakova;113(11):97-9, 2013.
[Is] ISSN:1997-7298
[Cp] País de publicação:Russia (Federation)
[La] Idioma:rus
[Mh] Termos MeSH primário: Deanol
Glutamatos
Nootrópicos
[Mh] Termos MeSH secundário: Animais
Disponibilidade Biológica
Encéfalo/metabolismo
Deanol/administração & dosagem
Deanol/farmacocinética
Deanol/uso terapêutico
Esquema de Medicação
Glutamatos/administração & dosagem
Glutamatos/farmacocinética
Glutamatos/uso terapêutico
Seres Humanos
Nootrópicos/administração & dosagem
Nootrópicos/farmacocinética
Nootrópicos/uso terapêutico
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Glutamates); 0 (Nootropic Agents); 2N6K9DRA24 (Deanol); 2PP737Z523 (deanol aceglutamate)
[Em] Mês de entrada:1407
[Cu] Atualização por classe:161018
[Lr] Data última revisão:
161018
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140117
[St] Status:MEDLINE


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[PMID]:24269909
[Au] Autor:Guo Y; Meng L; Zhang Y; Tang W; Zhang W; Xia Y; Ban F; Wu N; Zhang S
[Ad] Endereço:College of Chemistry & Molecular Engineering, Zhengzhou University, Zhengzhou 450052, PR China.
[Ti] Título:Sensitive determination of four tetracycline antibiotics in pig plasma by field-amplified sample stacking open-tubular capillary electrochromatography with dimethylethanolamine aminated polychloromethyl styrene nano-latex coated capillary column.
[So] Source:J Chromatogr B Analyt Technol Biomed Life Sci;942-943:151-7, 2013 Dec 30.
[Is] ISSN:1873-376X
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:This paper described the preparation and application of a new dimethylethanolamine aminated polychloromethyl styrene nano-latex (DMEAPL) coated capillary column (ccc-DMEAPL) in the determination of four tetracycline antibiotics (TCA) including tetracycline (TC), oxytetracycline (OTC), doxycycline (DC) and chlorotetracycline (CTC) in pig plasma. The ccc-DMEAPL column was characterized with steady EOF values of ca. 1.5-5.2×10(-5)cm(2)/Vs at pH 1.8-6.3. The optimized conditions for field-amplified sample stacking open-tubular capillary electrochromatography (FASS-OT-CEC) were as following: background electrolyte, 10mmol/L Na2HPO4+15mmol/L citric acid (pH 3.2); ccc-DMEAPL, 50µm i.d.×50cm (effective length 41.5cm), separation voltage, 18kV; column temperature, 25°C; UV detection wavelength, 270nm; water-plug injection: 30mbar×10s; sample electrokinetic injection, 10kV×20s. The four TCA were extracted with the solution of 10mmol/L Na2HPO4+15mmol/L citric acid+4g/L EDTA-2Na (pH 3.2). The FASS-OT-CEC method was validated in terms of linearity, sensitivity, selectivity, precision and accuracy. The LODs ranged from 3 to 7ng/mL, the recoveries for the four TCA were all more than 80%. The developed method was successfully applied for the determination of TCs in the actual pig plasma samples.
[Mh] Termos MeSH primário: Antibacterianos/sangue
Eletrocromatografia Capilar/instrumentação
Eletrocromatografia Capilar/métodos
Deanol/química
Nanopartículas/química
Tetraciclinas/sangue
[Mh] Termos MeSH secundário: Animais
Antibacterianos/química
Látex/química
Limite de Detecção
Modelos Lineares
Poliestirenos/química
Reprodutibilidade dos Testes
Suínos
Tetraciclinas/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Latex); 0 (Polystyrenes); 0 (Tetracyclines); 2N6K9DRA24 (Deanol)
[Em] Mês de entrada:1407
[Cu] Atualização por classe:131202
[Lr] Data última revisão:
131202
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:131126
[St] Status:MEDLINE


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[PMID]:23665261
[Au] Autor:Rodnick ME; Brooks AF; Hockley BG; Henderson BD; Scott PJ
[Ad] Endereço:Division of Nuclear Medicine, Department of Radiology, The University of Michigan Medical School, Ann Arbor, MI 48109, USA.
[Ti] Título:A fully-automated one-pot synthesis of [18F]fluoromethylcholine with reduced dimethylaminoethanol contamination via [18F]fluoromethyl tosylate.
[So] Source:Appl Radiat Isot;78:26-32, 2013 Aug.
[Is] ISSN:1872-9800
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:INTRODUCTION: A novel one-pot method for preparing [(18)F]fluoromethylcholine ([(18)F]FCH) via in situ generation of [(18)F]fluoromethyl tosylate ([(18)F]FCH2OTs), and subsequent [(18)F]fluoromethylation of dimethylaminoethanol (DMAE), has been developed. METHODS: [(18)F]FCH was prepared using a GE TRACERlab FXFN, although the method should be readily adaptable to any other fluorine-(18) synthesis module. Initially ditosylmethane was fluorinated to generate [(18)F]FCH2OTs. DMAE was then added and the reaction was heated at 120 °C for 10 min to generate [(18)F]FCH. After this time, reaction solvent was evaporated, and the crude reaction mixture was purified by solid-phase extraction using C(18)-Plus and CM-Light Sep-Pak cartridges to provide [(18)F]FCH formulated in USP saline. The formulated product was passed through a 0.22 µm filter into a sterile dose vial, and submitted for quality control testing. Total synthesis time was 1.25 h from end-of-bombardment. RESULTS: Typical non-decay-corrected yields of [(18)F]FCH prepared using this method were 91 mCi (7% non-decay corrected based upon ~1.3 Ci [(18)F]fluoride), and doses passed all other quality control (QC) tests. CONCLUSION: A one-pot liquid-phase synthesis of [(18)F]FCH has been developed. Doses contain extremely low levels of residual DMAE (31.6 µg/10 mL dose or ~3 ppm) and passed all other requisite QC testing, confirming their suitability for use in clinical imaging studies.
[Mh] Termos MeSH primário: Colina/análogos & derivados
Deanol/química
Deanol/isolamento & purificação
Contaminação de Medicamentos/prevenção & controle
Marcação por Isótopo/instrumentação
Robótica/instrumentação
[Mh] Termos MeSH secundário: Colina/síntese química
Colina/isolamento & purificação
Desenho de Equipamento
Análise de Falha de Equipamento
Marcação por Isótopo/métodos
Robótica/métodos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL
[Nm] Nome de substância:
0 (fluoromethylcholine); 2N6K9DRA24 (Deanol); N91BDP6H0X (Choline)
[Em] Mês de entrada:1311
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:130514
[St] Status:MEDLINE


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[PMID]:23001649
[Au] Autor:Watkins AJ; Roussel EG; Webster G; Parkes RJ; Sass H
[Ad] Endereço:School of Earth and Ocean Sciences, Cardiff University, Cardiff, United Kingdom.
[Ti] Título:Choline and N,N-dimethylethanolamine as direct substrates for methanogens.
[So] Source:Appl Environ Microbiol;78(23):8298-303, 2012 Dec.
[Is] ISSN:1098-5336
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Choline (N,N,N-trimethylethanolamine), which is widely distributed in membrane lipids and is a component of sediment biota, has been shown to be utilized anaerobically by mixed prokaryote cultures to produce methane but not by pure cultures of methanogens. Here, we show that five recently isolated Methanococcoides strains from a range of sediments (Aarhus Bay, Denmark; Severn Estuary mudflats at Portishead, United Kingdom; Darwin Mud Volcano, Gulf of Cadiz; Napoli mud volcano, eastern Mediterranean) can directly utilize choline for methanogenesis producing ethanolamine, which is not further metabolized. Di- and monomethylethanolamine are metabolic intermediates that temporarily accumulate. Consistent with this, dimethylethanolamine was shown to be another new growth substrate, but monomethylethanolamine was not. The specific methanogen inhibitor 2-bromoethanesulfonate (BES) inhibited methane production from choline. When choline and trimethylamine are provided together, diauxic growth occurs, with trimethylamine being utilized first, and then after a lag (∼7 days) choline is metabolized. Three type strains of Methanococcoides (M. methylutens, M. burtonii, and M. alaskense), in contrast, did not utilize choline. However, two of them (M. methylutens and M. burtonii) did metabolize dimethylethanolamine. These results extend the known substrates that can be directly utilized by some methanogens, giving them the advantage that they would not be reliant on bacterial syntrophs for their substrate supply.
[Mh] Termos MeSH primário: Colina/metabolismo
Deanol/metabolismo
Microbiologia Ambiental
Metano/metabolismo
Methanosarcinaceae/isolamento & purificação
Methanosarcinaceae/metabolismo
[Mh] Termos MeSH secundário: DNA Arqueal/química
DNA Arqueal/genética
DNA Ribossômico/química
DNA Ribossômico/genética
Etanolamina/metabolismo
Methanosarcinaceae/classificação
Methanosarcinaceae/genética
Dados de Sequência Molecular
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Archaeal); 0 (DNA, Ribosomal); 0 (RNA, Ribosomal, 16S); 2N6K9DRA24 (Deanol); 5KV86114PT (Ethanolamine); N91BDP6H0X (Choline); OP0UW79H66 (Methane)
[Em] Mês de entrada:1304
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:120925
[St] Status:MEDLINE
[do] DOI:10.1128/AEM.01941-12



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