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Pesquisa : D02.033.100.291.410 [Categoria DeCS]
Referências encontradas : 146 [refinar]
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  1 / 146 MEDLINE  
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[PMID]:27881619
[Au] Autor:Decheng S; Ruiguo W; Peilong W; Genlong Z; Yufei F; Xia F; Yang L; Xiaoou S
[Ad] Endereço:Institute of Quality Standards and Testing Technology for Agricultural Products, Chinese Academy of Agricultural Science, No. 12 South Street, Zhongguancun, Beijing 100081, China.
[Ti] Título:Accumulation and Determination of Phenylethanolamine A Residue in Hair of Swine and Sheep.
[So] Source:J Anal Toxicol;41(2):146-152, 2017 Mar 01.
[Is] ISSN:1945-2403
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The present study proposed the use of liquid chromatography-tandem mass spectrometry to detect the novel ß-agonist phenylethanolamine A (PEA) in incurred hair samples of swine and sheep and to assess its accumulation for residue monitoring. The method showed good percent recoveries ranging from 93.2% to 102% and good coefficient of variation at <15%. The experiment was conducted in swine (24 treated and 4 controls) and sheep (3 treated and 1 control). PEA concentration was determined in hair during the treatment. High residue concentrations were present in hair as early as Day 24 (14.8 ± 3.6 and 25.8 ± 7.6 ng/g) and Day 21 (23.4 ± 6.6 ng/g) for swine and sheep; these residues persisted until withdrawal on Days 14 and 21. Results showed high PEA accumulation in hair, thereby indicating the use of hair as a matrix in the control of PEA abuse in farm animals.
[Mh] Termos MeSH primário: 2-Hidroxifenetilamina/análogos & derivados
Agonistas Adrenérgicos beta/análise
Resíduos de Drogas/análise
Cabelo/química
Detecção do Abuso de Substâncias/métodos
Detecção do Abuso de Substâncias/veterinária
[Mh] Termos MeSH secundário: 2-Hidroxifenetilamina/análise
Animais
Cromatografia Líquida
Feminino
Masculino
Ovinos
Detecção do Abuso de Substâncias/instrumentação
Suínos
Espectrometria de Massas em Tandem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (2-(4-(nitrophenyl)butan-2-ylamino)-1-(4-methoxyphenyl)ethanol); 0 (Adrenergic beta-Agonists); 7568-93-6 (2-Hydroxyphenethylamine)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170525
[Lr] Data última revisão:
170525
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161125
[St] Status:MEDLINE
[do] DOI:10.1093/jat/bkw121


  2 / 146 MEDLINE  
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[PMID]:27247162
[Au] Autor:Jiang D; Cao B; Wang M; Yang H; Zhao K; Li J; Li M; Sun L; Deng A
[Ad] Endereço:The Key Lab of Health Chemistry & Molecular Diagnosis of Suzhou, College of Chemistry, Chemical Engineering and Materials Science, Soochow University, Suzhou 215123, China.
[Ti] Título:Development of a highly sensitive and specific monoclonal antibody based enzyme-linked immunosorbent assay for the detection of a new ß-agonist, phenylethanolamine A, in food samples.
[So] Source:J Sci Food Agric;97(3):1001-1009, 2017 Feb.
[Is] ISSN:1097-0010
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: All ß-agonists are banned as feed additives for growth promotion in animals due to toxic effects on humans after consuming the ß-agonist contaminated meats. Phenylethanolamine A (PA) is a newly emerged ß-agonist. Thus there is a need to develop highly sensitive and specific analytical methods for the detection of PA in food samples. In this study, the monoclonal antibody (mAb) against PA was produced by hybridoma technology and used for the development of enzyme-linked immunosorbent assay (ELISA). RESULTS: The IC values and limits of detection (LODs) of the ELISA using homogeneous combination of coating antigen/antibody for PA were 0.16 ng mL and 0.011 ng mL , respectively. The cross-reactive (CR) values of the assay with 14 structurally related ß-agonists were lower than 0.59%. Swine liver and meat samples were spiked with PA at different content and analysed by ELISA. Acceptable recovery rates of 91.40-105.51% and intra-assay coefficients of variation of 1.56-9.92% (n = 3) were obtained. The ELISA for seven spiked samples was confirmed by LC-MS/MS with a high correlation coefficient of 0.9881. CONCLUSION: The proposed mAb-based ELISA was highly sensitive and specific for PA and could be used as a quantitative/screening method for PA analysis in food samples. © 2016 Society of Chemical Industry.
[Mh] Termos MeSH primário: 2-Hidroxifenetilamina/análogos & derivados
Agonistas Adrenérgicos beta/análise
Anticorpos Monoclonais/metabolismo
Especificidade de Anticorpos
Resíduos de Drogas/análise
Ensaio de Imunoadsorção Enzimática
Inspeção de Alimentos/métodos
[Mh] Termos MeSH secundário: 2-Hidroxifenetilamina/análise
2-Hidroxifenetilamina/química
2-Hidroxifenetilamina/metabolismo
Agonistas Adrenérgicos beta/química
Agonistas Adrenérgicos beta/metabolismo
Animais
China
Cromatografia Líquida de Alta Pressão
Reações Cruzadas
Resíduos de Drogas/química
Resíduos de Drogas/metabolismo
Contaminação de Alimentos
Haptenos/química
Haptenos/metabolismo
Limite de Detecção
Fígado/química
Carne/análise
Estrutura Molecular
Reprodutibilidade dos Testes
Espectrometria de Massas por Ionização por Electrospray
Sus scrofa
Espectrometria de Massas em Tandem
[Pt] Tipo de publicação:JOURNAL ARTICLE; VALIDATION STUDIES
[Nm] Nome de substância:
0 (2-(4-(nitrophenyl)butan-2-ylamino)-1-(4-methoxyphenyl)ethanol); 0 (Adrenergic beta-Agonists); 0 (Antibodies, Monoclonal); 0 (Haptens); 7568-93-6 (2-Hydroxyphenethylamine)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170613
[Lr] Data última revisão:
170613
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160602
[St] Status:MEDLINE
[do] DOI:10.1002/jsfa.7826


  3 / 146 MEDLINE  
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[PMID]:27916457
[Au] Autor:Romero-Hernandez A; Simorowski N; Karakas E; Furukawa H
[Ad] Endereço:WM Keck Structural Biology Laboratory, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA; Watson School of Biological Sciences, Cold Spring Harbor, NY 11724, USA.
[Ti] Título:Molecular Basis for Subtype Specificity and High-Affinity Zinc Inhibition in the GluN1-GluN2A NMDA Receptor Amino-Terminal Domain.
[So] Source:Neuron;92(6):1324-1336, 2016 Dec 21.
[Is] ISSN:1097-4199
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Zinc is vastly present in the mammalian brain and controls functions of various cell surface receptors to regulate neurotransmission. A distinctive characteristic of N-methyl-D-aspartate (NMDA) receptors containing a GluN2A subunit is that their ion channel activity is allosterically inhibited by a nano-molar concentration of zinc that binds to an extracellular domain called an amino-terminal domain (ATD). Despite physiological importance, the molecular mechanism underlying the high-affinity zinc inhibition has been incomplete because of the lack of a GluN2A ATD structure. Here we show the first crystal structures of the heterodimeric GluN1-GluN2A ATD, which provide the complete map of the high-affinity zinc-binding site and reveal distinctive features from the ATD of the GluN1-GluN2B subtype. Perturbation of hydrogen bond networks at the hinge of the GluN2A bi-lobe structure affects both zinc inhibition and open probability, supporting the general model in which the bi-lobe motion in ATD regulates the channel activity in NMDA receptors.
[Mh] Termos MeSH primário: Receptores de N-Metil-D-Aspartato/metabolismo
Zinco/metabolismo
[Mh] Termos MeSH secundário: 2-Hidroxifenetilamina/metabolismo
Animais
Sítios de Ligação
Western Blotting
Cristalografia
Ligações de Hidrogênio
Piperidinas/farmacologia
Estrutura Quaternária de Proteína
Receptores de N-Metil-D-Aspartato/efeitos dos fármacos
Células Sf9
Spodoptera
Xenopus laevis
Zinco/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (N-methyl D-aspartate receptor subtype 2A); 0 (NR2B NMDA receptor); 0 (Piperidines); 0 (Receptors, N-Methyl-D-Aspartate); 7568-93-6 (2-Hydroxyphenethylamine); J41CSQ7QDS (Zinc); R8OE3P6O5S (ifenprodil)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170726
[Lr] Data última revisão:
170726
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161206
[St] Status:MEDLINE


  4 / 146 MEDLINE  
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[PMID]:26828267
[Au] Autor:Xi Y; Niu J; Shen Y; Li D; Peng X; Wu X
[Ad] Endereço:Tongji Hospital, Tongji Medical School, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China; Department of General Surgery, First Affiliated Hospital, School of Medicine, Shihezi University, Shihezi, Xinjiang 832008, China.
[Ti] Título:AT13148, a first-in-class multi-AGC kinase inhibitor, potently inhibits gastric cancer cells both in vitro and in vivo.
[So] Source:Biochem Biophys Res Commun;478(1):330-336, 2016 09 09.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The AGC kinase family is important cell proliferation and survival. Dysregulation of this family contributes to gastric cancer progression. Here, we evaluated the potential activity of AT13148, a first-in-class multi-AGC kinase inhibitor, against gastric cancer cells. Our results showed that AT13148 exerted potent cytotoxic and anti-proliferative activities against a panel human gastric cancer cell lines (HGC-27, AGS, SNU-601, N87 and MKN-28), possibly via inducing cancer cell apoptotic death. Apoptosis inhibition by the Caspase blockers dramatically attenuated AT13148-caused cytotoxicity against gastric cancer cells. Intriguingly, same AT13148 treatment was not cytotoxic/pro-apoptotic to the non-cancerous human gastric epithelial GEC-1 cells. At the signaling level, AT13148 treatment in gastric cancer cells dramatically suppressed activation of multiple AGC kinases, including Akt (at p-Thr-308), p70S6 kinase (p70S6K), glycogen synthase kinase 3ß (GSK-3ß) and p90 ribosomal S6 kinase (RSK). Our in vivo studies demonstrated that daily oral gavage of AT13148 at well-tolerated doses significantly inhibited HGC27 xenograft tumor growth in nude mice. AGC activity was also dramatically decreased in AT13148-administrated HGC27 tumors. Therefore, targeting AGC kinases by AT13148 demonstrates superior anti-gastric cancer activity both in vitro and in vivo. The preclinical results of this study support the progression of this molecule into future evaluation as a valuable anti-gastric cancer candidate.
[Mh] Termos MeSH primário: 2-Hidroxifenetilamina/análogos & derivados
Apoptose/efeitos dos fármacos
Sobrevivência Celular/efeitos dos fármacos
Proteínas Quinases/metabolismo
Pirazóis/administração & dosagem
Neoplasias Gástricas/tratamento farmacológico
Neoplasias Gástricas/metabolismo
[Mh] Termos MeSH secundário: 2-Hidroxifenetilamina/administração & dosagem
Administração Oral
Animais
Antineoplásicos/administração & dosagem
Linhagem Celular Tumoral
Relação Dose-Resposta a Droga
Feminino
Seres Humanos
Camundongos
Camundongos Endogâmicos BALB C
Camundongos Nus
Inibidores de Proteínas Quinases/administração & dosagem
Neoplasias Gástricas/patologia
Resultado do Tratamento
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (AT13148); 0 (Antineoplastic Agents); 0 (Protein Kinase Inhibitors); 0 (Pyrazoles); 7568-93-6 (2-Hydroxyphenethylamine); EC 2.7.- (Protein Kinases)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:171127
[Lr] Data última revisão:
171127
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160202
[St] Status:MEDLINE


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[PMID]:26179292
[Au] Autor:Bi S; Zhao T; Wang Y; Zhou H
[Ad] Endereço:College of Chemistry, Changchun Normal University, Changchun, 130032, China.
[Ti] Título:Study on the interactions of mapenterol with serum albumins using multi-spectroscopy and molecular docking.
[So] Source:Luminescence;31(2):372-9, 2016 Mar.
[Is] ISSN:1522-7243
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The interactions of mapenterol with bovine serum albumin (BSA) and human serum albumin (HSA) have been investigated systematically using fluorescence spectroscopy, absorption spectroscopy, circular dichroism (CD) and molecular docking techniques. Mapenterol has a strong ability to quench the intrinsic fluorescence of BSA and HSA through static quenching procedures. At 291 K, the binding constants, Ka, were 1.93 × 10(3) and 2.73 × 10(3) L/mol for mapenterol-BSA and mapenterol-HAS, respectively. Electrostatic forces and hydrophobic interactions played important roles in stabilizing the mapenterol-BSA/has complex. Using site marker competitive studies, mapenterol was found to bind at Sudlow site I on BSA/HSA. There was little effect of K(+), Ca(2+), Cu(2+), Zn(2+) and Fe(3+) on the binding. The conformation of BSA/HSA was changed by mapenterol, as seen from the synchronous fluorescence spectra. The CD spectra showed that the binding of mapenterol to BSA/HSA changed the secondary structure of BSA/HSA. Molecular docking further confirmed that mapenterol could bind to Sudlow site I of BSA/HSA. According to Förster non-radiative energy transfer theory (FRET), the distances r0 between the donor and acceptor were calculated as 3.18 and 2.75 nm for mapenterol-BSA and mapenterol-HAS, respectively.
[Mh] Termos MeSH primário: 2-Hidroxifenetilamina/análogos & derivados
Compostos de Anilina/química
Simulação de Acoplamento Molecular
Albumina Sérica/química
[Mh] Termos MeSH secundário: 2-Hidroxifenetilamina/química
Animais
Bovinos
Dicroísmo Circular
Seres Humanos
Espectrometria de Fluorescência
Espectrofotometria Ultravioleta
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Aniline Compounds); 0 (Serum Albumin); 0 (mapenterol); 7568-93-6 (2-Hydroxyphenethylamine)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170113
[Lr] Data última revisão:
170113
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150717
[St] Status:MEDLINE
[do] DOI:10.1002/bio.2969


  6 / 146 MEDLINE  
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[PMID]:26255292
[Au] Autor:Li X; Wang W; Wang L; Wang Q; Pei X; Jiang H
[Ad] Endereço:College of Veterinary Medicine, China Agricultural University, No. 2 Yuanmingyuan West Road, Beijing, 100193, China.
[Ti] Título:Rapid determination of phenylethanolamine A in biological samples by enzyme-linked immunosorbent assay and lateral-flow immunoassay.
[So] Source:Anal Bioanal Chem;407(25):7615-24, 2015 Oct.
[Is] ISSN:1618-2650
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Phenylethanolamine A (PA) is a ß-adrenergic agonist, which was first used in animal husbandry as a growth promoter in China in 2010. In this study, a monoclonal-antibody (mAb)-based indirect competitive enzyme-linked immunosorbent assay (icELISA) and lateral-flow immunoassay (LFA) for the detection of PA in swine urine and pork were developed. The immunogen was prepared by linking PA hapten with carrier protein via a diazotization method. The IC50 value of the optimized icELISA was 0.44 ng mL(-1). The limits of detection of the icELISA for PA in swine urine and pork were 0.13 ng mL(-1) and 0.39 ng g(-1), respectively. The recoveries of PA from spiked swine urine and pork were in the range 82.0-107.4 % and 81.8-113.3%, respectively, with the coefficients of variation in the range 4.1-16.2% and 1.2-6.3%, respectively. The mAbs had negligible cross reactivity with 10 other ß-agonists. In contrast, the LFA had a cut-off level of 5 ng mL(-1) (g) in swine urine and pork, and the results could be achieved within 5 min. Ten blind samples of swine urine were analyzed simultaneously by icELISA, LFA, and ultra-high-performance liquid chromatography-tandem mass spectrometry, and the results of the three methods agreed well. Therefore, the combination of two immunoassays provides an effective and rapid screening method for detection of PA residues in biological samples.
[Mh] Termos MeSH primário: 2-Hidroxifenetilamina/análogos & derivados
Agonistas Adrenérgicos beta/análise
Agonistas Adrenérgicos beta/urina
Ensaio de Imunoadsorção Enzimática/métodos
Substâncias de Crescimento/análise
Carne Vermelha/análise
Suínos/urina
[Mh] Termos MeSH secundário: 2-Hidroxifenetilamina/análise
2-Hidroxifenetilamina/imunologia
2-Hidroxifenetilamina/urina
Agonistas Adrenérgicos beta/imunologia
Animais
Anticorpos Monoclonais/imunologia
Ensaio de Imunoadsorção Enzimática/instrumentação
Desenho de Equipamento
Substâncias de Crescimento/urina
Limite de Detecção
Camundongos
Fitas Reagentes/análise
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (2-(4-(nitrophenyl)butan-2-ylamino)-1-(4-methoxyphenyl)ethanol); 0 (Adrenergic beta-Agonists); 0 (Antibodies, Monoclonal); 0 (Growth Substances); 0 (Reagent Strips); 7568-93-6 (2-Hydroxyphenethylamine)
[Em] Mês de entrada:1607
[Cu] Atualização por classe:150919
[Lr] Data última revisão:
150919
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150810
[St] Status:MEDLINE
[do] DOI:10.1007/s00216-015-8917-6


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[PMID]:26187072
[Au] Autor:Min W; Li Y; Zhang Y; Dai D; Cao Y; Yue Z; Liu J
[Ad] Endereço:Department of Neurosurgery, Changhai Hospital, Second Military Medical University, Shanghai 200433, China.
[Ti] Título:Role of the anti-glioma drug AT13148 in the inhibition of Notch signaling pathway.
[So] Source:Gene;573(1):153-9, 2015 Nov 15.
[Is] ISSN:1879-0038
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:OBJECTIVE: To investigate the drug targets related to Notch signaling pathway for glioma treatment. METHODS: Gene expression profiles GSE44561, GSE48079 and GSE22772GSE48079GSE22772 of glioma cells samples with activated Notch signaling pathway and control samples were downloaded from Gene Expression Omnibus database to screen the differentially expressed genes (DEGs) using limma package. GO (Gene Oncology) function and KEGG (Kyoto Encyclopedia of Genes and Genomes) pathway enrichment analyses were conducted using DAVID tools to predict the underlying function of these DEGs. Sequentially, drug target genes recorded in DrugBank database were collected and matched with the selected DEGs to identify the potential drug targets for glioma. Further, these targets were verified by the screened DEGs in the anti-glioma drug (AT13148) treated samples of microarray data of GSE38008. RESULTS: A total of 75,645,497 DEGs were respectively identified in GSE44561, GSE48079 and GSE22772GSE48079GSE22772 datasets and these DEGs could well distinguish the glioma samples from controls. The DEGs were mainly enriched in classical functions and pathways, such as cell cycle, and DNA replication. A total of 122 DEGs were found to be potential drug targets for glioma, among which GLIPR1 was targeted by drug XL820, PDGFRB and KDR were targeted by SOT-107. Efficacy validation of the other 119 drug targets by GSE38008 data showed that ACSS1, ASL, GCLM, ROCK2, IMPA1, and TFPI may be targeted by the anti-glioma drug of AT13148. CONCLUSION: AT13148 may inhibit glioma progression by suppressing the Notch signaling genes, including GLIPR1, PDGFRB, ACSS1, and ASL.
[Mh] Termos MeSH primário: 2-Hidroxifenetilamina/análogos & derivados
Antineoplásicos/farmacologia
Neoplasias Encefálicas/patologia
Glioma/patologia
Pirazóis/farmacologia
Receptores Notch/metabolismo
[Mh] Termos MeSH secundário: 2-Hidroxifenetilamina/farmacologia
Neoplasias Encefálicas/metabolismo
Glioma/metabolismo
Seres Humanos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (AT13148); 0 (Antineoplastic Agents); 0 (Pyrazoles); 0 (Receptors, Notch); 7568-93-6 (2-Hydroxyphenethylamine)
[Em] Mês de entrada:1512
[Cu] Atualização por classe:150924
[Lr] Data última revisão:
150924
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150719
[St] Status:MEDLINE


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[PMID]:25840982
[Au] Autor:Sadok A; McCarthy A; Caldwell J; Collins I; Garrett MD; Yeo M; Hooper S; Sahai E; Kuemper S; Mardakheh FK; Marshall CJ
[Ad] Endereço:Division of Cancer Biology, Institute of Cancer Research, London, United Kingdom. chris.marshall@icr.ac.uk amine.sadok@icr.ac.uk.
[Ti] Título:Rho kinase inhibitors block melanoma cell migration and inhibit metastasis.
[So] Source:Cancer Res;75(11):2272-84, 2015 Jun 01.
[Is] ISSN:1538-7445
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:There is an urgent need to identify new therapeutic opportunities for metastatic melanoma. Fragment-based screening has led to the discovery of orally available, ATP-competitive AKT kinase inhibitors, AT13148 and CCT129254. These compounds also inhibit the Rho-kinases ROCK 1 and ROCK 2 and we show they potently inhibit ROCK activity in melanoma cells in culture and in vivo. Treatment of melanoma cells with CCT129254 or AT13148 dramatically reduces cell invasion, impairing both "amoeboid-like" and mesenchymal-like modes of invasion in culture. Intravital imaging shows that CCT129254 or AT13148 treatment reduces the motility of melanoma cells in vivo. CCT129254 inhibits melanoma metastasis when administered 2 days after orthotopic intradermal injection of the cells, or when treatment starts after metastases have arisen. Mechanistically, our data suggest that inhibition of ROCK reduces the ability of melanoma cells to efficiently colonize the lungs. These results suggest that these novel inhibitors of ROCK may be beneficial in the treatment of metastasis.
[Mh] Termos MeSH primário: 2-Hidroxifenetilamina/análogos & derivados
Movimento Celular/efeitos dos fármacos
Melanoma/tratamento farmacológico
Inibidores de Proteínas Quinases/administração & dosagem
Pirazóis/administração & dosagem
Quinases Associadas a rho/genética
[Mh] Termos MeSH secundário: 2-Hidroxifenetilamina/administração & dosagem
Linhagem Celular Tumoral
Seres Humanos
Melanoma/genética
Melanoma/patologia
Invasividade Neoplásica/genética
Metástase Neoplásica
Fosforilação
Transdução de Sinais/efeitos dos fármacos
Quinases Associadas a rho/antagonistas & inibidores
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (AT13148); 0 (Protein Kinase Inhibitors); 0 (Pyrazoles); 7568-93-6 (2-Hydroxyphenethylamine); EC 2.7.11.1 (rho-Associated Kinases)
[Em] Mês de entrada:1508
[Cu] Atualização por classe:170922
[Lr] Data última revisão:
170922
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150405
[St] Status:MEDLINE
[do] DOI:10.1158/0008-5472.CAN-14-2156


  9 / 146 MEDLINE  
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[PMID]:25343225
[Au] Autor:Li M; Yang H; Li S; Zhao K; Li J; Jiang D; Sun L; Deng A
[Ad] Endereço:The Key Laboratory of Health Chemistry and Molecular Diagnosis of Suzhou, College of Chemistry, Chemical Engineering and Materials Science, and ‡College of Pharmacy Sciences, Soochow University , Suzhou 215123, China.
[Ti] Título:Ultrasensitive and quantitative detection of a new ß-agonist phenylethanolamine A by a novel immunochromatographic assay based on surface-enhanced Raman scattering (SERS).
[So] Source:J Agric Food Chem;62(45):10896-902, 2014 Nov 12.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Phenylethanolamine A (PA) is a new kind of ß-agonist, which was illegally used as a feed additive for growth promotion in China. In this study, a novel immunochromatographic assay (ICA) based on surface-enhanced Raman scattering (SERS) for the ultrasensitive and quantitative detection of phenylethanolamine A is presented. The principle of this new ICA is similar to that based on colloidal gold particles, but using Au(MBA)@Ag-Ab [e.g., polyclonal antibody of PA labeled Au@Ag core-shell nanoparticles (NPs) sandwiched with a Raman reporter (4-mercaptobenzoic acid, MBA)] as a probe. After ICA procedures, the specific Raman scattering intensity of MBA on the test line was measured for quantitative detection of PA. This assay was completed within 15 min. The IC50 and limit of detection (LOD) values of the ICA for PA detection were 0.06 ng mL(-1) and 0.32 pg mL(-1), respectively, which were 1-3 orders of magnitude lower than those obtained by other immunoassays, indicating the ultrasensitivity of this ICA. There was no cross-reactivity (CR) of the assay with another three ß-agonists (ractopamine, clenbuterol, and salbutamol), suggesting high specificity of the SERS-based ICA. A spiking experiment revealed that the recoveries of PA from pig urine samples were in range of 99.9- 101.2% with relative standard deviations (RSDs) of 3.6-5.8%. The results demonstrated that this SERS-based ICA was able to quantitatively detect PA in urine samples with high sensitivity, specificity, precision, and accuracy and might be a powerful method for the analysis of other target analytes in the food area.
[Mh] Termos MeSH primário: 2-Hidroxifenetilamina/análise
Agonistas Adrenérgicos beta/análise
Imunocromatografia/métodos
Análise Espectral Raman/métodos
[Mh] Termos MeSH secundário: 2-Hidroxifenetilamina/urina
Agonistas Adrenérgicos beta/urina
Animais
Ouro/química
Imunocromatografia/instrumentação
Nanopartículas Metálicas/química
Sensibilidade e Especificidade
Suínos
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Adrenergic beta-Agonists); 7440-57-5 (Gold); 7568-93-6 (2-Hydroxyphenethylamine)
[Em] Mês de entrada:1506
[Cu] Atualização por classe:141113
[Lr] Data última revisão:
141113
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:141025
[St] Status:MEDLINE
[do] DOI:10.1021/jf503599x


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[PMID]:25011489
[Au] Autor:Yan P; Zhang J; Tang Q; Deng A; Li J
[Ad] Endereço:College of Chemistry, Chemical Engineering & Materials Science, Soochow University, Suzhou 215123, China. lijgsd@suda.edu.cn denganping@suda.edu.cn.
[Ti] Título:A quantum dot based electrochemiluminescent immunosensor for the detection of pg level phenylethanolamine A using gold nanoparticles as substrates and electron transfer accelerators.
[So] Source:Analyst;139(17):4365-72, 2014 Sep 07.
[Is] ISSN:1364-5528
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:This study reports the development of an electrochemiluminescent (ECL) immunosensor for ultrasensitive detection of phenylethanolamine A (PA) based on CdSe quantum dots (QDs) and gold nanoparticles (GNPs). The GNPs/ovalbumin-PA/anti-PA-QD immunosensor was fabricated layer by layer using GNPs as substrates and electron transport accelerators. The use of GNPs greatly enhanced the sensitivity for detecting PA due to the excellent electron transportation ability and the large surface area of GNP carriers allowing several binding events of ovalbumin-PA on each nanosphere. Transmission electron microscopy images (TEM), photoluminescence spectra, ultraviolet-visible absorption spectra and dynamic light scattering (DLS) were used to characterize the QDs and GNPs. The sensor was characterized with electrochemical impedance spectra (EIS), and a strong ECL emission of the modified electrode could be observed during the cathodic process of S2O8(2-) and QDs in air-saturated PBS buffer containing 0.1 M K2S2O8 and 0.1 M KCl (pH 7.4). With a competitive immunoassay format, the ECL signal depended linearly on the logarithm of the phenylethanolamine A concentration within a range of 0.02 ng mL(-1) to 50 ng mL(-1), and the detection limit was 0.0047 ng mL(-1), much lower than those reported in the literature. This ECL immunosensor is rapid, simple and sensitive with acceptable precision, and it will extend the application of QD ECL in immunoassays of ß-agonists and open new avenues for the detection of food additive residues in the future.
[Mh] Termos MeSH primário: 2-Hidroxifenetilamina/análise
Agonistas Adrenérgicos beta/análise
Ouro/química
Imunoensaio/métodos
Medições Luminescentes/métodos
Nanopartículas Metálicas/química
Pontos Quânticos/química
[Mh] Termos MeSH secundário: 2-Hidroxifenetilamina/análogos & derivados
2-Hidroxifenetilamina/urina
Agonistas Adrenérgicos beta/urina
Animais
Anticorpos Imobilizados/química
Técnicas Biossensoriais/métodos
Compostos de Cádmio/química
Técnicas Eletroquímicas/métodos
Seres Humanos
Limite de Detecção
Carne/análise
Compostos de Selênio/química
Suínos
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Adrenergic beta-Agonists); 0 (Antibodies, Immobilized); 0 (Cadmium Compounds); 0 (Selenium Compounds); 7440-57-5 (Gold); 7568-93-6 (2-Hydroxyphenethylamine); A7F646JC5C (cadmium selenide)
[Em] Mês de entrada:1508
[Cu] Atualização por classe:140729
[Lr] Data última revisão:
140729
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140712
[St] Status:MEDLINE
[do] DOI:10.1039/c4an00378k



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