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[PMID]:26851866
[Au] Autor:Azizi K; Koli MG
[Ad] Endereço:Department of Chemistry, University of Kurdistan, Sanandaj, Iran; Research center of Nanotechnology, University of Kurdistan, 66177-15175, Sanandaj, Iran. Electronic address: k.azizi@uok.ac.ir.
[Ti] Título:Molecular dynamics simulations of Oxprenolol and Propranolol in a DPPC lipid bilayer.
[So] Source:J Mol Graph Model;64:153-164, 2016 Mar.
[Is] ISSN:1873-4243
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Extensive microscopic molecular dynamics simulations have been performed to study the effects of tow ß-blocker drugs (Propranolol, Oxprenolol) on fully hydrated dipalmitoylphosphatidylcholine (DPPC) in the fluid phase at 323K. Simulation of 4 systems containing varying concentrations of drugs was carried out. For the purpose of comparison, a fully hydrated DPPC bilayer without drugs was also studied at the same level of simulation technique which has been done on 4 other systems. The length of each simulation was 100ns. The effects of concentrations of both drugs were analyzed on lipid bilayer properties, such as electrostatic potential, order parameter, diffusion coefficients, and hydrogen bond formation, etc. Penetration of water in the bilayer system was also investigated using radial distribution function analysis. Efficacy of varying concentrations of both drugs has no significant effect on P-N vector. Consistent with experimental results, by increasing the concentration of Propranolol, the thickness of the bilayer was increased.
[Mh] Termos MeSH primário: 1,2-Dipalmitoilfosfatidilcolina/química
Bicamadas Lipídicas/química
Simulação de Dinâmica Molecular
Oxprenolol/química
Propranolol/química
[Mh] Termos MeSH secundário: Antagonistas Adrenérgicos beta/química
Ligações de Hidrogênio
Conformação Molecular
Estrutura Molecular
Eletricidade Estática
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Adrenergic beta-Antagonists); 0 (Lipid Bilayers); 2644-64-6 (1,2-Dipalmitoylphosphatidylcholine); 519MXN9YZR (Oxprenolol); 9Y8NXQ24VQ (Propranolol)
[Em] Mês de entrada:1611
[Cu] Atualização por classe:170610
[Lr] Data última revisão:
170610
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160208
[St] Status:MEDLINE


  2 / 1000 MEDLINE  
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[PMID]:26460233
[Au] Autor:Santos MG; Moraes Gde O; Nakamura MG; dos Santos-Neto ÁJ; Figueiredo EC
[Ad] Endereço:Toxicants and Drugs Analysis Laboratory - LATF, Faculty of Pharmaceutical Sciences, Federal University of Alfenas - Unifal-MG, 700 Gabriel Monteiro da Silva street, 37130-000, Alfenas, MG, Brazil. eduardocfig@yahoo.com.br.
[Ti] Título:Restricted access molecularly imprinted polymers obtained by bovine serum albumin and/or hydrophilic monomers' external layers: a comparison related to physical and chemical properties.
[So] Source:Analyst;140(22):7768-75, 2015 Nov 21.
[Is] ISSN:1364-5528
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Molecularly imprinting polymers (MIPs) can be modified with external layers in order to obtain restricted access molecularly imprinted polymers (RAMIPs) able to exclude macromolecules and retain low weight compounds. These modifications have been frequently achieved using hydrophilic monomers, chemically bound on the MIP surface. Recently, our group proposed a new biocompatible RAMIP based on the formation of a bovine serum albumin coating on the surface of MIP particles. This material has been used to extract drugs directly from untreated human plasma samples, but its physicochemical evaluation has not been carried out yet, mainly in comparison with RAMIPs obtained by hydrophilic monomers. Thus, we proposed in this paper a comparative study involving the surface composition, microscopic aspect, selectivity, binding kinetics, adsorption and macromolecule elimination ability of these different materials. We concluded that the synthesis procedure influences the size and shape of particles and that hydrophilic co-monomer addition as well as coating with BSA do not alter the chemical recognition ability of the material. The difference between imprinted and non-imprinted polymers' adsorption was evident (suggesting that imprinted polymers have a better capacity to bind the template than the non-imprinted ones). The Langmuir model presents the best fit to describe the materials' adsorption profile. The polymer covered with hydrophilic monomers presented the best adsorption for the template in an aqueous medium, probably due to a hydrophilic layer on its surface. We also concluded that an association of the hydrophilic monomers with the bovine serum albumin coating is important to obtain materials with higher capacity of macromolecule exclusion.
[Mh] Termos MeSH primário: Antagonistas Adrenérgicos beta/isolamento & purificação
Impressão Molecular/métodos
Oxprenolol/isolamento & purificação
Polímeros/química
Soroalbumina Bovina/química
[Mh] Termos MeSH secundário: Adsorção
Animais
Bovinos
Seres Humanos
Interações Hidrofóbicas e Hidrofílicas
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Adrenergic beta-Antagonists); 0 (Polymers); 27432CM55Q (Serum Albumin, Bovine); 519MXN9YZR (Oxprenolol)
[Em] Mês de entrada:1607
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151014
[St] Status:MEDLINE
[do] DOI:10.1039/c5an01482d


  3 / 1000 MEDLINE  
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[PMID]:24727142
[Au] Autor:Amores S; Lauroba J; Calpena A; Colom H; Gimeno A; Domenech J
[Ad] Endereço:Pharmacy Pharmaceutical Technology Department, Biopharmaceutical Pharmacokinetics Unit, Faculty of Pharmacy, Av. Joan XXIII, s/n, Barcelona 08028, Spain.
[Ti] Título:A comparative ex vivo drug permeation study of beta-blockers through porcine buccal mucosa.
[So] Source:Int J Pharm;468(1-2):50-4, 2014 Jul 01.
[Is] ISSN:1873-3476
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Apparent permeability coefficients (kp) of a series of beta-blockers: acebutolol, atenolol, labetalol, metoprolol, oxprenolol and propranolol, through porcine buccal mucosa were determined. The aim of the study was to determine the permeation parameters (apparent permeability coefficient, kp; flux, J; and lag time, TL) as a measure of the intrinsic permeability of porcine buccal mucosa to these drugs, in order to predict the efficacy of their possible administration through human buccal mucosa. A positive linear correlation was observed between the apparent permeability coefficient, kpand the partition coefficient, P. Oxprenolol and propranolol are the drugs that presented the highest values of kp: 0.3231×10(2) cm/h and 0.5666×10(2) cm/h, respectively. Multiple linear regression (MLR) using least square estimation was performed on the data set with logkpas dependent variable and the descriptors as predictor variables. The potential systemic capacity after a buccal administration was predicted by estimating the plasma concentrations at steady-stated (Css). Considering the entire process of permeation ex vivo, propranolol and oxprenolol would seem to be the best candidates for administration through the buccal mucosa.
[Mh] Termos MeSH primário: Antagonistas Adrenérgicos beta/metabolismo
Mucosa Bucal/metabolismo
[Mh] Termos MeSH secundário: Administração Bucal
Antagonistas Adrenérgicos beta/administração & dosagem
Antagonistas Adrenérgicos beta/química
Animais
Transporte Biológico
Seres Humanos
Técnicas In Vitro
Análise dos Mínimos Quadrados
Modelos Lineares
Modelos Biológicos
Estrutura Molecular
Oxprenolol/metabolismo
Permeabilidade
Propranolol/metabolismo
Relação Estrutura-Atividade
Suínos
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Adrenergic beta-Antagonists); 519MXN9YZR (Oxprenolol); 9Y8NXQ24VQ (Propranolol)
[Em] Mês de entrada:1501
[Cu] Atualização por classe:140527
[Lr] Data última revisão:
140527
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140415
[St] Status:MEDLINE


  4 / 1000 MEDLINE  
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[PMID]:24681296
[Au] Autor:Först G; Cwiklik L; Jurkiewicz P; Schubert R; Hof M
[Ad] Endereço:Institute of Pharmaceutical Sciences, Department of Pharmaceutical Technology and Biopharmacy, University of Freiburg, Freiburg, Germany. Electronic address: gesche@foerst.de.
[Ti] Título:Interactions of beta-blockers with model lipid membranes: molecular view of the interaction of acebutolol, oxprenolol, and propranolol with phosphatidylcholine vesicles by time-dependent fluorescence shift and molecular dynamics simulations.
[So] Source:Eur J Pharm Biopharm;87(3):559-69, 2014 Aug.
[Is] ISSN:1873-3441
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Since pharmacokinetic and pharmacodynamic activities of drugs are often related to their interactions with biomembranes, it is of high interest to establish an approach for the characterization of these interactions at the molecular level. For the present study, beta-blockers (oxprenolol, propranolol, and acebutolol) were selected due to their well described nonspecific membrane effects (NME). Their interactions with model lipid membranes composed of palmitoyloleoylphosphatidylcholine (POPC) were studied using Time-Dependent Fluorescence Shift (TDFS) and Generalized Polarization (GP) as well as molecular dynamics (MD) simulations. Liposomal vesicles were labeled with fluorescent membrane polarity probes (Laurdan, Prodan, and Dtmac). Increasing beta-blocker concentrations (0-10 mM for acebutolol and oxprenolol, and 0-1.5 mM for propranolol) significantly rigidifies the lipid bilayer at the glycerol and headgroup level, which was detected in the steady-state and in the time-resolved fluorescence data. The effects of propranolol were considerably stronger than those of the two other beta-blockers. The addition of fluorescent probes precisely located at different levels within the lipid bilayer revealed the insertion of the beta-blockers into the POPC bilayer at the glycerol backbone level, which was further confirmed by MD simulations in the case of propranolol.
[Mh] Termos MeSH primário: Acebutolol/metabolismo
Antagonistas Adrenérgicos beta/metabolismo
Bicamadas Lipídicas/metabolismo
Lipídeos de Membrana/metabolismo
Oxprenolol/metabolismo
Fosfatidilcolinas/metabolismo
Propranolol/metabolismo
[Mh] Termos MeSH secundário: Fluorescência
Corantes Fluorescentes/metabolismo
Glicerol/metabolismo
Lipossomos/metabolismo
Simulação de Dinâmica Molecular
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Adrenergic beta-Antagonists); 0 (Fluorescent Dyes); 0 (Lipid Bilayers); 0 (Liposomes); 0 (Membrane Lipids); 0 (Phosphatidylcholines); 519MXN9YZR (Oxprenolol); 67P356D8GH (Acebutolol); 9Y8NXQ24VQ (Propranolol); PDC6A3C0OX (Glycerol); TE895536Y5 (1-palmitoyl-2-oleoylphosphatidylcholine)
[Em] Mês de entrada:1503
[Cu] Atualização por classe:140714
[Lr] Data última revisão:
140714
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140401
[St] Status:MEDLINE


  5 / 1000 MEDLINE  
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[PMID]:22284473
[Au] Autor:Jin Y; Chen C; Meng L; Chen J; Li M; Zhu Z
[Ad] Endereço:Beijing National Laboratory for Molecular Sciences, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, PR China.
[Ti] Título:Simultaneous and sensitive capillary electrophoretic enantioseparation of three ß-blockers with the combination of achiral ionic liquid and dual CD derivatives.
[So] Source:Talanta;89:149-54, 2012 Jan 30.
[Is] ISSN:1873-3573
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Successful simultaneous enantioseparation and sensitive determination of three ß-blockers (PIN, OX and PRO), have been achieved by capillary electrophoresis using an achiral ionic liquid, [GTMA]Cl, as a modifier to cooperate with dual CDs containing DM-ß-CD and TM-ß-CD. The influence of aIL was investigated in details, including various aILs, the concentration of aIL and molar ratio of aIL to CD. The ratio of DM-ß-CD to TM-ß-CD in dual CDs was also discussed. DM-ß-CD and TM-ß-CD favor the enantioseparations of PIN/OX and PRO, respectively. Meanwhile, the presence of [GTMA]Cl was found to play a key role in enantioseparations, and it widened the scope of application of DM-ß-CD and TM-ß-CD. Furthermore, FESI as an effective on-line sample enrichment technique was developed to improve the detection sensitivity. Under the optimum conditions, the detection limits of the three pairs of enantiomers range from 0.10 to 0.65 nM, which are much lower than those in the conventional methods. Eventually, the proposed method was successfully applied to the analysis of spiked urine sample with good recoveries.
[Mh] Termos MeSH primário: Antagonistas Adrenérgicos beta/urina
Ciclodextrinas/química
Eletroforese Capilar/métodos
Líquidos Iônicos/química
[Mh] Termos MeSH secundário: Seres Humanos
Concentração de Íons de Hidrogênio
Limite de Detecção
Oxprenolol/urina
Pindolol/urina
Propranolol/urina
Estereoisomerismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Adrenergic beta-Antagonists); 0 (Cyclodextrins); 0 (Ionic Liquids); 519MXN9YZR (Oxprenolol); 9Y8NXQ24VQ (Propranolol); BJ4HF6IU1D (Pindolol)
[Em] Mês de entrada:1206
[Cu] Atualização por classe:131121
[Lr] Data última revisão:
131121
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:120131
[St] Status:MEDLINE
[do] DOI:10.1016/j.talanta.2011.12.005


  6 / 1000 MEDLINE  
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[PMID]:20601889
[Au] Autor:Mizogami M; Takakura K; Tsuchiya H
[Ad] Endereço:Department of Anesthesiology, Asahi University School of Dentistry, Gifu, Japan. makikai@dent.asahiu.ac.jp
[Ti] Título:The interactivities with lipid membranes differentially characterize selective and nonselective beta1-blockers.
[So] Source:Eur J Anaesthesiol;27(9):829-34, 2010 Sep.
[Is] ISSN:1365-2346
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND AND OBJECTIVE: beta-Adrenoceptor-blocking agents have been used for perioperative management during anaesthesia, in which selective beta1-blockers are advantageous over nonselective beta-blockers. Apart from the different affinity for beta-adrenoceptors, beta1-blockers were differentially characterized in light of their different interaction with lipid membranes. METHODS: Selective (atenolol, metoprolol and esmolol) and nonselective (alprenolol, oxprenolol and propranolol) beta1-blockers were reacted at 0.2-1 mmol l with 1,2-dipalmitoylphosphatidylcholine liposomes and biomimetic membranes consisting of phospholipids, sphingolipid and cholesterol. Their membrane interactivities were comparatively determined using the potency to modify membrane fluidity by measuring fluorescence polarization. Their relative hydrophobicities were evaluated by reversed-phase liquid chromatography. RESULTS: The chromatographic evaluation divided the tested drugs into more hydrophobic ones containing nonselective beta-blockers and less hydrophobic ones containing selective beta1-blockers. Nonselective beta-blockers, but not selective beta1-blockers, fluidized liposomal membranes, with the potency being oxprenolol < alprenolol < propranolol. Membrane-active alprenolol preferentially acted on the hydrophobic deeper regions of phospholipid bilayers. The potency of nonselective beta-blockers to fluidize biomimetic membranes was greatest in propranolol, followed by alprenolol and oxprenolol, whereas all selective beta1-blockers were inactive. CONCLUSION: The membrane-fluidizing effects of beta-blockers are correlated with their relative hydrophobicities and their respective conformations to perturb the alignment of phospholipid acyl chains. The membrane-interacting characteristics differentiate beta-blockers as nonselective propranolol, alprenolol and oxprenolol vs. beta1-selective atenolol, metoprolol and esmolol. Such differentiation reflects not only the structural difference but also the beta-adrenoceptor-blocking difference. The membrane fluidization may be partly responsible for the nonselective blockade of beta-adrenoceptors.
[Mh] Termos MeSH primário: Antagonistas Adrenérgicos beta/química
Anestésicos/uso terapêutico
Bicamadas Lipídicas/química
Lipídeos/química
[Mh] Termos MeSH secundário: Alprenolol/farmacologia
Anestesia
Atenolol/farmacologia
Biomimética
Lipossomos/química
Membranas Artificiais
Metoprolol/farmacologia
Oxprenolol/farmacologia
Propanolaminas/farmacologia
Propranolol/farmacologia
Receptores Adrenérgicos beta/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Adrenergic beta-Antagonists); 0 (Anesthetics); 0 (Lipid Bilayers); 0 (Lipids); 0 (Liposomes); 0 (Membranes, Artificial); 0 (Propanolamines); 0 (Receptors, Adrenergic, beta); 50VV3VW0TI (Atenolol); 519MXN9YZR (Oxprenolol); 877K5MQ27W (Alprenolol); 9Y8NXQ24VQ (Propranolol); GEB06NHM23 (Metoprolol); MDY902UXSR (esmolol)
[Em] Mês de entrada:1011
[Cu] Atualização por classe:131121
[Lr] Data última revisão:
131121
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:100706
[St] Status:MEDLINE
[do] DOI:10.1097/EJA.0b013e32833bf5e4


  7 / 1000 MEDLINE  
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[PMID]:20128736
[Au] Autor:Salman SA; Sulaiman SA; Ismail Z; Gan SH
[Ad] Endereço:Department of Pharmacology, School of Medical Sciences, Universiti Sains Malaysia, 16150, Kubang Kerian, Kelantan, Malaysia.
[Ti] Título:Quantitative determination of propranolol by ultraviolet HPLC in human plasma.
[So] Source:Toxicol Mech Methods;20(3):137-42, 2010 Mar.
[Is] ISSN:1537-6524
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Many previous published methods for the quantitative determination of propranolol (PRN) in human plasma have poor recoveries and were not validated according to the FDA guideline. The aim of this study is to develop a simple HPLC method for detecting PRN in human plasma and to validate it so that it can be applied to a clinical study. Chromatographic separation was achieved using a mixture of a mobile phase consisting of 160 ml water, 180 ml methanol, 70 ml acetonitrile, 2.5 ml acetic acid, and 125 microl triethylamine (v/v). The pH of the whole mixture was adjusted to 3.4. A flow rate of 0.5 ml/min was employed throughout with a 15 microl injection volume. Detection was done using a UV detector at 291 nm. The validated method was linear for concentrations ranging from 15-180 ng/ ml with a good separation and specificity for both PRN and its internal standard, oxprenolol (OXP), with excellent recoveries, precision, and accuracies. The limit of detection (LOD) and limit of quantification (LOQ) were 1 and 10 ng/ml, respectively. The stability studies demonstrated that PRN is stable in the autosampler vials and also up to 3.5 months. To the authors' knowledge, the recovery, that ranged between 97.9-102.7%, is the highest among all previously reported methods that used HPLC with UV detection. The developed and validated method for PRN analysis is excellent and applicable to a clinical study.
[Mh] Termos MeSH primário: Antagonistas Adrenérgicos beta/sangue
Cromatografia Líquida de Alta Pressão/métodos
Propranolol/sangue
Raios Ultravioleta
[Mh] Termos MeSH secundário: Antagonistas Adrenérgicos beta/química
Seres Humanos
Oxprenolol/sangue
Propranolol/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; VALIDATION STUDIES
[Nm] Nome de substância:
0 (Adrenergic beta-Antagonists); 519MXN9YZR (Oxprenolol); 9Y8NXQ24VQ (Propranolol)
[Em] Mês de entrada:1004
[Cu] Atualização por classe:131121
[Lr] Data última revisão:
131121
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:100205
[St] Status:MEDLINE
[do] DOI:10.3109/15376511003602112


  8 / 1000 MEDLINE  
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[PMID]:19348017
[Au] Autor:Groening R; Bensmann H
[Ad] Endereço:Institute of Pharmaceutical Technology and Biopharmaceutics, Westfälische Wilhelms-Universität Münster, Münster, Germany. groenin@uni-muenster.de
[Ti] Título:High frequency controlled capsules with integrated gas producing cells.
[So] Source:Eur J Pharm Biopharm;72(1):282-4, 2009 May.
[Is] ISSN:1873-3441
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:In the present investigations, a new high frequency remote-controlled capsule has been developed in which the mechanical energy to empty a drug reservoir is generated by a miniature gas producing cell. If the poles of the gas producing cell are connected by an electric circuit, the gas production starts. The rate of gas production can be regulated by a resistor in the electric circuit and by the duration of activation of the system. To get a remote control, we developed a small receiver which is located inside the capsule. The receiver consists of an oscillating circuit, which is in resonance with an external 24 MHz high frequency transmitter. A MOSFET transistor acts as a switch in the electric circuit to start the gas production. Release experiments with oxprenolol show that different release patterns can be obtained.
[Mh] Termos MeSH primário: Cápsulas
Tecnologia Farmacêutica/instrumentação
[Mh] Termos MeSH secundário: Química Farmacêutica/instrumentação
Química Farmacêutica/métodos
Portadores de Fármacos
Composição de Medicamentos
Sistemas de Liberação de Medicamentos
Eletroquímica/métodos
Desenho de Equipamento
Gases
Oxprenolol/química
Solubilidade
Tecnologia Farmacêutica/métodos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Capsules); 0 (Drug Carriers); 0 (Gases); 519MXN9YZR (Oxprenolol)
[Em] Mês de entrada:0909
[Cu] Atualização por classe:131121
[Lr] Data última revisão:
131121
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:090407
[St] Status:MEDLINE
[do] DOI:10.1016/j.ejpb.2009.01.003


  9 / 1000 MEDLINE  
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[PMID]:18781297
[Au] Autor:Buszewski B; Welerowicz T; Tegowska E; Krzeminski TF
[Ad] Endereço:Department of Environmental Chemistry and Ecoanalytics, Faculty of Chemistry, Nicolas Copernicus University, Gagarin Street 7, 87-100, Torun, Poland. bbusz@chem.uni.torun.pl
[Ti] Título:Determination of selected beta-receptor antagonists in biological samples by solid-phase extraction with cholesterolic phase and LC/MS.
[So] Source:Anal Bioanal Chem;393(1):263-72, 2009 Jan.
[Is] ISSN:1618-2650
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:A new method is presented for the determination of five selected beta-receptor antagonists by HPLC, which emphasizes sample preparation via retention on a new type of silica gel sorbent used for solid-phase extraction (SPE). Sorbents of this type were obtained by the chemical modification of silica gels of various porosities by cholesterol ligands. The cholesterol-based packing material was investigated by spectroscopic methods and elemental analysis. The recoveries obtained with the extraction procedure were optimum over a relatively broad sample pH range (3.08-7.50). Analytical factors such as the sample loading, the washing step and elution conditions, the concentration of beta-receptor antagonists to be extracted, and the type of sorbent were found to play significant roles in the sample preparation procedure and would therefore need to be controlled to achieve optimum recoveries of the analytes. Under optimum conditions, the recoveries of nadolol, acebutolol, esmolol, oxprenolol and propranolol from spiked buffers, blood and urine were reproducible and dependent on the polarity or hydrophilicity of the compounds. The above analytes were determined by reverse-phase high-performance liquid chromatography (HPLC) with UV and ESI-ion trap mass spectrometry (MS) detection. The described method was found to be suitable for the routine measurement of compounds that are both polar and basic, and can be applied for the analysis of biological samples such as urine and blood in clinical, toxicological or forensic laboratories. The recovery measurements were performed on spiked human urine and serum, and on real samples of mouse blood serum.
[Mh] Termos MeSH primário: Acebutolol/análise
Colesterol/química
Nadolol/análise
Oxprenolol/análise
Propanolaminas/análise
Propranolol/análise
Extração em Fase Sólida/métodos
[Mh] Termos MeSH secundário: Acebutolol/sangue
Acebutolol/urina
Animais
Cromatografia Líquida de Alta Pressão
Seres Humanos
Masculino
Camundongos
Nadolol/sangue
Nadolol/urina
Oxprenolol/sangue
Oxprenolol/urina
Propanolaminas/sangue
Propanolaminas/urina
Propranolol/sangue
Propranolol/urina
Reprodutibilidade dos Testes
Dióxido de Silício/química
Espectrometria de Massas por Ionização por Electrospray
Espectrofotometria Ultravioleta
Propriedades de Superfície
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Propanolamines); 42200-33-9 (Nadolol); 519MXN9YZR (Oxprenolol); 67P356D8GH (Acebutolol); 7631-86-9 (Silicon Dioxide); 97C5T2UQ7J (Cholesterol); 9Y8NXQ24VQ (Propranolol); MDY902UXSR (esmolol)
[Em] Mês de entrada:0901
[Cu] Atualização por classe:160512
[Lr] Data última revisão:
160512
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:080911
[St] Status:MEDLINE
[do] DOI:10.1007/s00216-008-2369-1


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Fotocópia
[PMID]:19007474
[Au] Autor:Billiot E; Billiot F; Warner IM
[Ad] Endereço:Department of Physical and Life Sciences, Texas A&M Corpus Christi, Corpus Christi, TX 78412, USA. Eugene.Billiot@tamucc.edu
[Ti] Título:Optimization of 12 chiral analytes with 8 polymeric surfactants.
[So] Source:J Chromatogr Sci;46(9):757-63, 2008 Oct.
[Is] ISSN:0021-9665
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:This manuscript discusses the results of studies that were performed to determine optimum capillary electrophoresis (CE) conditions for the enantiomeric resolution of twelve chiral analytes with eight amino acid based polymeric surfactants. The parameters that were optimized include pH, buffer type, and concentration of surfactant. The results indicated that the optimum conditions for enantiomeric separations with the amino acid based polymeric surfactants examined in this study using CE were analyte dependent, not surfactant dependent. In other words, the optimum conditions for a particular analyte were the same for all the amino acid based polymeric surfactants examined in this study. The results of these studies indicate that when using a large group of related amino acid based polymeric surfactants only a few surfactants need to be optimized for each analyte under study. These studies were limited to anionic surfactants that contain the amino acids glycine, L-alanine, L-valine, and L-leucine only. No inference can be necessarily drawn about surfactants containing other types of amino acids such as threonine and serine, which contain extra heteroatoms, or phenylalanine that has an aromatic moiety.
[Mh] Termos MeSH primário: Aminoácidos/química
Benzodiazepinonas/isolamento & purificação
Eletroforese Capilar/métodos
Naftalenos/isolamento & purificação
Organofosfatos/isolamento & purificação
Propanolaminas/isolamento & purificação
Tensoativos/química
[Mh] Termos MeSH secundário: Alprenolol/isolamento & purificação
Tampões (Química)
Diaminas/isolamento & purificação
Concentração de Íons de Hidrogênio
Lorazepam/isolamento & purificação
Naftóis/isolamento & purificação
Oxazepam/isolamento & purificação
Oxprenolol/isolamento & purificação
Propranolol/isolamento & purificação
Estereoisomerismo
Temazepam/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (1,1'-binaphthyl-2,2'-diamine); 0 (Amino Acids); 0 (Benzodiazepinones); 0 (Buffers); 0 (Diamines); 0 (Naphthalenes); 0 (Naphthols); 0 (Organophosphates); 0 (Propanolamines); 0 (Surface-Active Agents); 35193-63-6 (1,1'-binaphthyl-2,2'-diyl hydrogen phosphate); 519MXN9YZR (Oxprenolol); 602-09-5 (1,1'-bi-2-naphthol); 6GOW6DWN2A (Oxazepam); 877K5MQ27W (Alprenolol); 9Y8NXQ24VQ (Propranolol); CHB1QD2QSS (Temazepam); O26FZP769L (Lorazepam)
[Em] Mês de entrada:0812
[Cu] Atualização por classe:131121
[Lr] Data última revisão:
131121
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:081115
[St] Status:MEDLINE



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