Base de dados : MEDLINE
Pesquisa : D02.033.260.100 [Categoria DeCS]
Referências encontradas : 386 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 39 ir para página                         

  1 / 386 MEDLINE  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29248572
[Au] Autor:Xiao C; He P; Han J; Tang M; Wang Z; Mi Y; Liu X
[Ad] Endereço:Laboratory of Functional Chemistry and Nutrition of Food, College of Food Science and Engineering, Northwest A&F University, Yangling, China.
[Ti] Título:1,3-Dichloro-2-propanol evokes inflammation and apoptosis in BV-2 microglia via MAPKs and NF-κB signaling pathways mediated by reactive oxygen species.
[So] Source:Toxicol Lett;284:103-112, 2018 Mar 01.
[Is] ISSN:1879-3169
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:1,3-dichloro-2-propanol (1,3-DCP) is a widely concerned food processing contaminant which has been investigated for decades. While the neurotoxicity of 1,3-DCP and related mechanisms are still elusive. Herein, the effect of 1,3-DCP on neurotoxicity was investigated using BV-2 microglia cells. 1,3-DCP significantly decreased cell viability from 78.6% to 59.2% at doses between 2 and 20 mM. AO/EB and JC-1 staining indicated that 1,3-DCP induced apoptosis by means of the decrease of mitochondrial membrane potential. Meanwhile, western blot showed that 1,3-DCP stimulated inflammation of BV-2 cells through phosphorylation of MAPKs and activation of NF-κB pathways mediated by reactive oxygen species (ROS). Furthermore, the degree of inflammation and apoptosis has eased through MAPKs and NF-κB pathways with cells pretreated by N-acetylcysteine (NAC). Overall, these results presented here suggested that 1,3-DCP has neurotoxic effect on BV-2 microglia mainly via MAPKs and NF-κB pathways mediated by ROS.
[Mh] Termos MeSH primário: Apoptose/efeitos dos fármacos
Microglia/efeitos dos fármacos
Proteínas Quinases Ativadas por Mitógeno/metabolismo
NF-kappa B/metabolismo
Espécies Reativas de Oxigênio/metabolismo
alfa-Cloridrina/análogos & derivados
[Mh] Termos MeSH secundário: Acetilcisteína/farmacologia
Animais
Linhagem Celular
Sobrevivência Celular/efeitos dos fármacos
Relação Dose-Resposta a Droga
Contaminação de Alimentos
Potencial da Membrana Mitocondrial/efeitos dos fármacos
Camundongos
Microglia/imunologia
Microglia/patologia
Fosforilação
Transdução de Sinais
alfa-Cloridrina/toxicidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (NF-kappa B); 0 (Reactive Oxygen Species); 0F4P2VQC07 (1,3-dichloro-2-propanol); 96-24-2 (alpha-Chlorohydrin); EC 2.7.11.24 (Mitogen-Activated Protein Kinases); WYQ7N0BPYC (Acetylcysteine)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180221
[Lr] Data última revisão:
180221
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171218
[St] Status:MEDLINE


  2 / 386 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29260544
[Au] Autor:Tiong SH; Saparin N; Teh HF; Ng TLM; Md Zain MZB; Neoh BK; Md Noor A; Tan CP; Lai OM; Appleton DR
[Ad] Endereço:Sime Darby Technology Centre Sdn. Bhd. , 1st Floor, Block B, UPM-MTDC Technology Centre III, Lebuh Silikon, 43400 Serdang, Selangor, Malaysia.
[Ti] Título:Natural Organochlorines as Precursors of 3-Monochloropropanediol Esters in Vegetable Oils.
[So] Source:J Agric Food Chem;66(4):999-1007, 2018 Jan 31.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:During high-temperature refining of vegetable oils, 3-monochloropropanediol (3-MCPD) esters, possible carcinogens, are formed from acylglycerol in the presence of a chlorine source. To investigate organochlorine compounds in vegetable oils as possible precursors for 3-MCPD esters, we tested crude palm, soybean, rapeseed, sunflower, corn, coconut, and olive oils for the presence of organochlorine compounds. Having found them in all vegetable oils tested, we focused subsequent study on oil palm products. Analysis of the chlorine isotope mass pattern exhibited in high-resolution mass spectrometry enabled organochlorine compound identification in crude palm oils as constituents of wax esters, fatty acid, diacylglycerols, and sphingolipids, which are produced endogenously in oil palm mesocarp throughout ripening. Analysis of thermal decomposition and changes during refining suggested that these naturally present organochlorine compounds in palm oils and perhaps in other vegetable oils are precursors of 3-MCPD esters. Enrichment and dose-response showed a linear relationship to 3-MCPD ester formation and indicated that the sphingolipid-based organochlorine compounds are the most active precursors of 3-MCPD esters.
[Mh] Termos MeSH primário: Hidrocarbonetos Clorados/química
Óleos Vegetais/química
alfa-Cloridrina/química
[Mh] Termos MeSH secundário: Carcinógenos
Cloro/química
Ésteres/química
Contaminação de Alimentos
Manipulação de Alimentos
Glicerídeos/química
Óleo de Palmeira/química
alfa-Cloridrina/análise
alfa-Cloridrina/síntese química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Carcinogens); 0 (Esters); 0 (Glycerides); 0 (Hydrocarbons, Chlorinated); 0 (Plant Oils); 4R7X1O2820 (Chlorine); 5QUO05548Z (Palm Oil); 96-24-2 (alpha-Chlorohydrin)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180212
[Lr] Data última revisão:
180212
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171221
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jafc.7b04995


  3 / 386 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29305303
[Au] Autor:Lu J; Cheng B; Meng Z; Fang B; Li T; Sun M; Liu M; Guan S
[Ad] Endereço:Food Science and Engineering College, Jilin University, Changchun, Jilin Province 130062, PR China.
[Ti] Título:Alliin attenuates 1, 3-dichloro-2-propanol-induced lipogenesis in HepG2 cells through activation of the AMP-activated protein kinase-dependent pathway.
[So] Source:Life Sci;195:19-24, 2018 Feb 15.
[Is] ISSN:1879-0631
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Accumulating evidence reveals the association of 1, 3-dichloro-2-propanol (1, 3-DCP) exposure and lipogenesis. Alliin, the most abundant sulphur compound in garlic, has been demonstrated to exhibit hypoglycemic, antioxidant and anti-inflammatory activities. Here, we showed that alliin attenuated lipogenesis induced by 1,3-DCP and that the reduction was due to activation of the AMPK pathway. HepG2 cells exposed to 1,3-DCP exhibited significant increase of triglyceride(TG) and total cholesterol(TC), and alliin reduced the accumulation. Most importantly, alliin could up-regulate the phosphorylation of AMPK and down-regulate protein and gene expressions of SREBP-1; FAS; SREBP-2;HMGCR in 1,3-DCP-induced HepG2 cells. The results demonstrated that alliin was effective on attenuating 1,3-DCP-induced lipogenesis via activation of the AMPK-SREBPs signaling pathway in HepG2 cells.
[Mh] Termos MeSH primário: Proteínas Quinases Ativadas por AMP/metabolismo
Cisteína/análogos & derivados
Hipolipemiantes/farmacologia
Metabolismo dos Lipídeos/efeitos dos fármacos
Transdução de Sinais/efeitos dos fármacos
alfa-Cloridrina/análogos & derivados
[Mh] Termos MeSH secundário: Sobrevivência Celular/efeitos dos fármacos
Colesterol/biossíntese
Cisteína/farmacologia
Ativação Enzimática/efeitos dos fármacos
Regulação da Expressão Gênica/efeitos dos fármacos
Células Hep G2
Seres Humanos
Proteínas de Ligação a Elemento Regulador de Esterol/biossíntese
Proteínas de Ligação a Elemento Regulador de Esterol/genética
Triglicerídeos/biossíntese
alfa-Cloridrina/antagonistas & inibidores
alfa-Cloridrina/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Hypolipidemic Agents); 0 (Sterol Regulatory Element Binding Proteins); 0 (Triglycerides); 0F4P2VQC07 (1,3-dichloro-2-propanol); 7I4L2D0E9G (alliin); 96-24-2 (alpha-Chlorohydrin); 97C5T2UQ7J (Cholesterol); EC 2.7.11.31 (AMP-Activated Protein Kinases); K848JZ4886 (Cysteine)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180208
[Lr] Data última revisão:
180208
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180107
[St] Status:MEDLINE


  4 / 386 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28820048
[Au] Autor:Graziani G; Gaspari A; Chianese D; Conte L; Ritieni A
[Ad] Endereço:a Department of Pharmacy , University of Naples "Federico II" , Napoli , Italy.
[Ti] Título:Direct determination of 3-chloropropanol esters in edible vegetable oils using high resolution mass spectrometry (HRMS-Orbitrap).
[So] Source:Food Addit Contam Part A Chem Anal Control Expo Risk Assess;34(11):1893-1903, 2017 Nov.
[Is] ISSN:1944-0057
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A series of refined edible oils derived from mixed seeds, peanuts, corn, sunflower and palm obtained from the local supermarket were analyzed for their content of 3-MCPD esters. A direct analytical method for the determination of 3-monochloropropane-1,2-diol esters (3-MCPD esters) was applied to investigate the major MCPD esters found in common edible oils; in particular seven types of monoesters and eleven types of diesters were detected. The limits of detection (LODs) for monoesters and diesters of 3-MCPD were in the range of 0.079-12.678 µg kg and 0.033-18.610 µg kg in edible oils, and the ranges of limits of quantitation (LOQs) were 0.979-38.035 µg kg and 0.100-55 µg kg , respectively. The recoveries of 3-MCPD esters from oil samples were in the range of 80-100%, with RSD ranging between 1.9 and 11.8%. The concentration levels of total 3-MCPD diesters in vegetable oil samples were in the range from 0.106 up to 3.444 µg g whereas total monoesters ranged from 0.005 up to 1.606 µg g .
[Mh] Termos MeSH primário: Ésteres/análise
Análise de Alimentos
Contaminação de Alimentos/análise
Óleos Vegetais/química
alfa-Cloridrina/análise
[Mh] Termos MeSH secundário: Espectrometria de Massas
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Esters); 0 (Plant Oils); 96-24-2 (alpha-Chlorohydrin)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171016
[Lr] Data última revisão:
171016
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170819
[St] Status:MEDLINE
[do] DOI:10.1080/19440049.2017.1368721


  5 / 386 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28494640
[Au] Autor:Hung WC; Peng GJ; Tsai WJ; Chang MH; Liao CD; Tseng SH; Kao YM; Wang DY; Cheng HF
[Ad] Endereço:a Food and Drug Administration , Ministry of Health and Welfare, Executive Yuan , Taipei City , Taiwan.
[Ti] Título:Identification of 3-MCPD esters to verify the adulteration of extra virgin olive oil.
[So] Source:Food Addit Contam Part B Surveill;10(3):233-239, 2017 Sep.
[Is] ISSN:1939-3229
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The adulteration of olive oil is an important issue around the world. This paper reports an indirect method by which to identify 3-monochloropropane-1,2-diol (3-MCPD) esters in olive oils. Following sample preparation, the samples were spiked with 1,2-bis-palmitoyl-3-chloropropanediol standard for analysis using gas chromatograph-tandem mass spectrometry. The total recovery ranged from 102.8% to 105.5%, the coefficient of variation ranged from 1.1% to 10.1%, and the limit of quantification was 0.125 mg/kg. The content of 3-MCPD esters in samples of refined olive oil (0.97-20.53 mg/kg) exceeded those of extra virgin olive oil (non-detected to 0.24 mg/kg). These results indicate that the oil refining process increased the content of 3-MCPD esters, which means that they could be used as a target compound for the differentiation of extra virgin olive oil from refined olive oil in order to prevent adulteration.
[Mh] Termos MeSH primário: Análise de Alimentos/métodos
Contaminação de Alimentos/análise
Azeite de Oliva/química
alfa-Cloridrina/química
[Mh] Termos MeSH secundário: Cloretos
Reprodutibilidade dos Testes
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Chlorides); 0 (Olive Oil); 96-24-2 (alpha-Chlorohydrin)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170829
[Lr] Data última revisão:
170829
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170513
[St] Status:MEDLINE
[do] DOI:10.1080/19393210.2017.1330292


  6 / 386 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28351537
[Au] Autor:Yan N; Wan XF; Chai XS; Chen RQ
[Ad] Endereço:State Key Laboratory of Pulp and Paper Engineering, South China University of Technology, Guangzhou, China.
[Ti] Título:Determination of chlorinated volatile organic compounds in polyamine epichlorohydrin solution by headspace gas chromatography.
[So] Source:J Chromatogr A;1496:163-166, 2017 May 05.
[Is] ISSN:1873-3778
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:This study demonstrated a headspace gas chromatographic (HS-GC) method for the determination of residual epichlorohydrin (ECH) and the by-product 1,3-dichloro-2-propanol (DCP) in polyamine epichlorohydrin (PAE) solution. It was based on the vapor-liquid phase equilibrium of these analytes at 60°C for 30min in a closed headspace sample vial before GC measurement. It was found that matrix of the PAE solution had the effect on the headspace equilibrium of these species and therefore a standard addition must be applied in the method validation. The results showed that the present method has a good measurement precision (RSD <2.90%) and accuracy (recoveries from 93.6 to 105%), and the limit of quantitation (LOQ) is 3.75mg/L for ECH and 0.8g/L for DCP. The present method is suitable to be used for analyzing the chlorinated volatile organic compounds in the commercial PAE resin solutions.
[Mh] Termos MeSH primário: Cromatografia Gasosa
Epicloroidrina/análise
Poliaminas/química
Compostos Orgânicos Voláteis/análise
[Mh] Termos MeSH secundário: Limite de Detecção
Soluções/química
alfa-Cloridrina/análogos & derivados
alfa-Cloridrina/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Polyamines); 0 (Solutions); 0 (Volatile Organic Compounds); 08OOR508C0 (Epichlorohydrin); 0F4P2VQC07 (1,3-dichloro-2-propanol); 96-24-2 (alpha-Chlorohydrin)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170516
[Lr] Data última revisão:
170516
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170330
[St] Status:MEDLINE


  7 / 386 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28276235
[Au] Autor:Gao B; Liu M; Huang G; Zhang Z; Zhao Y; Wang TT; Zhang Y; Liu J; Yu L
[Ad] Endereço:Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology & Business University (BTBU) , Beijing 100048, China.
[Ti] Título:Absorption, Distribution, Metabolism and Excretion of 3-MCPD 1-Monopalmitate after Oral Administration in Rats.
[So] Source:J Agric Food Chem;65(12):2609-2614, 2017 Mar 29.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Fatty acid esters of monochloropropane 1,2-diol (3-MCPD) are processing-induced toxicants and have been detected in several food categories. This study investigated the absorption, distribution, metabolism, and excretion of 3-MCPD esters in Sprague-Dawley (SD) rats using 3-MCPD 1-monopalmitate as the probe compound. The kinetics of 3-MCPD 1-monopalmitate in plasma was investigated using SD rats, and the results indicated that 3-MCPD 1-monopalmitate was absorbed directly in vivo and metabolized. Its primary metabolites in the liver, kidney, testis, brain, plasma, and urine were tentatively identified and measured at 6, 12, 24, and 48 h after oral administration. Structures were proposed for eight metabolites. 3-MCPD 1-monopalmitate was converted to free 3-MCPD, which formed the phase II metabolites. All of the metabolites were chlorine-related chemical components; most of them existed in urine, reflecting the excretion pattern of 3-MCPD esters. Understanding the metabolism of 3-MCPD esters in vivo is critical for assessing their toxicities.
[Mh] Termos MeSH primário: Palmitatos/farmacocinética
alfa-Cloridrina/farmacocinética
[Mh] Termos MeSH secundário: Administração Oral
Animais
Ésteres/administração & dosagem
Ésteres/farmacocinética
Ácidos Graxos/química
Ácidos Graxos/metabolismo
Rim/metabolismo
Fígado/metabolismo
Masculino
Palmitatos/administração & dosagem
Ratos
Ratos Sprague-Dawley
Testículo/metabolismo
alfa-Cloridrina/administração & dosagem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Esters); 0 (Fatty Acids); 0 (Palmitates); 96-24-2 (alpha-Chlorohydrin)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170627
[Lr] Data última revisão:
170627
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170310
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jafc.7b00639


  8 / 386 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28074221
[Au] Autor:Wu Z; Deng W; Tong Y; Liao Q; Xin D; Yu H; Feng J; Tang L
[Ad] Endereço:School of Life Science and Technology, University of Electronic Science and Technology of China, No. 4, Section 2, North Jianshe Road, Chengdu, 610054, China.
[Ti] Título:Exploring the thermostable properties of halohydrin dehalogenase from Agrobacterium radiobacter AD1 by a combinatorial directed evolution strategy.
[So] Source:Appl Microbiol Biotechnol;101(8):3201-3211, 2017 Apr.
[Is] ISSN:1432-0614
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:As a crucial factor for biocatalysts, protein thermostability often arises from a combination of factors that are often difficult to rationalize. In this work, the thermostable nature of halohydrin dehalogenase from Agrobacterium radiobacter AD1 (HheC) was systematically explored using a combinatorial directed evolution approach. For this, a mutagenesis library of HheC mutants was first constructed using error-prone PCR with low mutagenesis frequency. After screening approximately 2000 colonies, six mutants with eight mutation sites were obtained. Those mutation sites were subsequently combined by adopting several rounds of iterative saturation mutagenesis (ISM) approach. After four rounds of saturation mutagenesis, one best mutant ISM-4 with a 3400-fold improvement in half-life (t ) inactivation at 65 °C, 18 °C increase in apparent T value, and 20 °C increase in optimum temperature was obtained, compared to wild-type HheC. To the best of our knowledge, the mutant represents the most thermostable HheC variant reported up to now. Moreover, the mutant was as active as wild-type enzyme for the substrate 1,3-dichloro-2-propanol, and they remained most enantioselectivity of wild-type enzyme in the kinetic resolution of rac-2-chloro-1-phenolethanol, exhibiting a great potential for industrial applications. Our structural investigation highlights that surface loop regions are hot spots for modulating the thermostability of HheC, the residues located at these regions contribute to the thermostability of HheC in a cooperative way, and protein rigidity and oligomeric interface connections contribute to the thermostability of HheC. All of these essential factors could be used for further design of an even more thermostable HheC, which, in turn, could greatly facilitate the application of the enzyme as a biocatalyst.
[Mh] Termos MeSH primário: Agrobacterium tumefaciens/genética
Evolução Molecular Direcionada/métodos
Hidrolases/genética
Hidrolases/metabolismo
[Mh] Termos MeSH secundário: Agrobacterium tumefaciens/enzimologia
Biocatálise
Estabilidade Enzimática
Biblioteca Gênica
Hidrolases/química
Cinética
Modelos Moleculares
Mutagênese
Mutação
Reação em Cadeia da Polimerase
Temperatura Ambiente
alfa-Cloridrina/análogos & derivados
alfa-Cloridrina/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0F4P2VQC07 (1,3-dichloro-2-propanol); 96-24-2 (alpha-Chlorohydrin); EC 3.- (Hydrolases); EC 3.8.1.- (halohydrin dehalogenase)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170425
[Lr] Data última revisão:
170425
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170112
[St] Status:MEDLINE
[do] DOI:10.1007/s00253-017-8090-2


  9 / 386 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28070108
[Au] Autor:Ji J; Zhu P; Sun C; Sun J; An L; Zhang Y; Sun X
[Ad] Endereço:State Key Laboratory of Food Science and Technology, School of Food Science of Jiangnan University, School of Food Science Synergetic Innovation Center of Food Safety and Nutrition, China.
[Ti] Título:Pathway of 3-MCPD-induced apoptosis in human embryonic kidney cells.
[So] Source:J Toxicol Sci;42(1):43-52, 2017.
[Is] ISSN:1880-3989
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:3-Chloropropane-1,2-diol (3-MCPD) is a heat-produced contaminant formed during the preparation of soy sauce worldwide. The present investigation was conducted to determine the molecular aspects of 3-MCPD toxicity on human embryonic kidney cells (HEK293). Cell viability and apoptosis were assessed in response to exposure to 3-MCPD using the MTT assay and high-content screening (HCS). DNA damage, intracellular reactive oxygen species (ROS) and apoptosis-related proteins were evaluated. Genes related with apoptosis were detected by qPCR-array for further understanding the 3-MCPD induced cell apoptosis signaling pathway. Our results clearly showed that 3-MCPD treatment inhibits cell proliferation and reactive oxygen species generation. qPCR-array indicated that nine apoptotic genes were up-regulated more than 2-fold and six down-regulated more than 2-fold. Genes associated with the mitochondrial apoptotic pathway, especially BCL2 family genes, changed significantly, indicating that the mitochondrial apoptotic pathway is activated. Death receptor pathway-related genes, TNFRSF11B and TNFRSF1A, changed significantly, indicating that the death receptor pathway is also activated, resulting in the inhibition of cell growth and proliferation as well as induction of apoptosis. To sum up, the experiment results indicated that 3-MCPD induced HEK293 cell toxicity through the death receptor pathway and mitochondrial pathway.
[Mh] Termos MeSH primário: Apoptose/efeitos dos fármacos
alfa-Cloridrina/toxicidade
[Mh] Termos MeSH secundário: Apoptose/genética
Apoptose/fisiologia
Caspases/metabolismo
Ciclo Celular/efeitos dos fármacos
Sobrevivência Celular/efeitos dos fármacos
Citocromos c/metabolismo
Dano ao DNA
Contaminação de Alimentos
Células HEK293
Seres Humanos
Osteoprotegerina/genética
Estresse Oxidativo/efeitos dos fármacos
Proteínas Proto-Oncogênicas c-bcl-2/genética
Proteínas Proto-Oncogênicas c-bcl-2/metabolismo
Espécies Reativas de Oxigênio/metabolismo
Receptores Tipo I de Fatores de Necrose Tumoral/genética
Proteína Supressora de Tumor p53/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Osteoprotegerin); 0 (Proto-Oncogene Proteins c-bcl-2); 0 (Reactive Oxygen Species); 0 (Receptors, Tumor Necrosis Factor, Type I); 0 (TNFRSF11B protein, human); 0 (TNFRSF1A protein, human); 0 (Tumor Suppressor Protein p53); 9007-43-6 (Cytochromes c); 96-24-2 (alpha-Chlorohydrin); EC 3.4.22.- (Caspases)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170523
[Lr] Data última revisão:
170523
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170111
[St] Status:MEDLINE
[do] DOI:10.2131/jts.42.43


  10 / 386 MEDLINE  
              first record previous record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28070102
[Au] Autor:Toyoda T; Cho YM; Akagi JI; Mizuta Y; Matsushita K; Nishikawa A; Imaida K; Ogawa K
[Ad] Endereço:Division of Pathology, National Institute of Health Sciences.
[Ti] Título:Altered susceptibility of an obese rat model to 13-week subchronic toxicity induced by 3-monochloropropane-1,2-diol.
[So] Source:J Toxicol Sci;42(1):1-11, 2017.
[Is] ISSN:1880-3989
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:3-Monochloropropane-1,2-diol (3-MCPD) is a heat-induced food contaminant that has been shown to be a nongenotoxic renal carcinogen. Although the toxicity of 3-MCPD has been widely investigated for decades, there is a further concern that 3-MCPD might exert more potent toxicity in high-risk population with underlying diseases such as hyperlipidemia associated with obesity. In the present study, we performed a 13-week subchronic toxicity study for 3-MCPD using an obesity rat model to investigate the differences in susceptibility between obese and normal individuals. Male F344 and obese Zucker (lean and fatty) rats were administered 0, 9, 28.5, 90, 285, or 900 ppm 3-MCPD in drinking water for 13 weeks. 3-MCPD treatment decreased body weight gain, increased relative kidney weights, induced anemia, and induced epithelial cell necrosis in epididymal ducts in all 3 strains. The degrees of epididymal damage were higher in F344 and lean rats than in fatty rats, while renal toxicity was most potent in F344 rats and comparable in lean and fatty rats. In contrast, the hematology data indicated that anemia was worse in fatty rats than in F344 and lean rats, and a significant decrease in hematopoietic cells in the bone marrow was observed only in fatty rats. The no-observed-adverse-effect level was estimated to be 28.5 ppm in all 3 strains for 3-MCPD. These results suggested that obese Zucker rats may be more susceptible to 3-MCPD-dependent toxicity in the hematopoietic tissues than their lean counterparts.
[Mh] Termos MeSH primário: Obesidade
alfa-Cloridrina/toxicidade
[Mh] Termos MeSH secundário: Animais
Modelos Animais de Doenças
Epididimo/efeitos dos fármacos
Epididimo/patologia
Contaminação de Alimentos
Testes Hematológicos
Rim/efeitos dos fármacos
Rim/patologia
Masculino
Nível de Efeito Adverso não Observado
Obesidade/sangue
Obesidade/patologia
Ratos Endogâmicos F344
Ratos Zucker
Testes de Toxicidade Subcrônica
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
96-24-2 (alpha-Chlorohydrin)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170523
[Lr] Data última revisão:
170523
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170111
[St] Status:MEDLINE
[do] DOI:10.2131/jts.42.1



página 1 de 39 ir para página                         
   


Refinar a pesquisa
  Base de dados : MEDLINE Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde