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[PMID]:28252357
[Au] Autor:Larsen T; Fernández C
[Ad] Endereço:Department of Animal Science,Aarhus University,Blichers Allé 20,PO Box 50,8830 Tjele,Denmark.
[Ti] Título:Enzymatic-fluorometric analyses for glutamine, glutamate and free amino groups in protein-free plasma and milk.
[So] Source:J Dairy Res;84(1):32-35, 2017 Feb.
[Is] ISSN:1469-7629
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:This Technical Research Communication describes new analytical methods for free, unbound glutamic acid and glutamine in protein-free blood plasma and milk and introduces the use of quantitation of free amino groups in the same matrices for descriptive and analytical purposes. The present enzymatic-fluorometric methods are easily performed within one working day, allowing for 'high throughput' assays of animal trials. These assays could support and enable further studies in lactation physiology with the objective of improved metabolic health.
[Mh] Termos MeSH primário: Aminoácidos/análise
Aminoácidos/sangue
Fluorometria/métodos
Ácido Glutâmico/análise
Glutamina/análise
Leite/química
[Mh] Termos MeSH secundário: Animais
Bovinos
Feminino
Ácido Glutâmico/sangue
Glutamina/sangue
Indicadores e Reagentes
Lactação
Mercaptoetanol/química
Reprodutibilidade dos Testes
o-Ftalaldeído/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Amino Acids); 0 (Indicators and Reagents); 0RH81L854J (Glutamine); 3KX376GY7L (Glutamic Acid); 60-24-2 (Mercaptoethanol); 643-79-8 (o-Phthalaldehyde)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170316
[Lr] Data última revisão:
170316
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170303
[St] Status:MEDLINE
[do] DOI:10.1017/S0022029916000789


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[PMID]:28214920
[Au] Autor:Dos Santos LS; Sá JC; Dos Santos Ribeiro DL; Chaves NP; da Silva Mol JP; Santos RL; da Paixão TA; de Carvalho Neta AV
[Ad] Endereço:Doutorado em Rede de Biodiversidade e Biotecnologia da Amazônia Legal, Universidade Estadual do Maranhão, São Luís, MA, Brazil.
[Ti] Título:Detection of Brucella sp. infection through serological, microbiological, and molecular methods applied to buffaloes in Maranhão State, Brazil.
[So] Source:Trop Anim Health Prod;49(4):675-679, 2017 Apr.
[Is] ISSN:1573-7438
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The aim of the current study is to diagnose Brucella spp. infection using methods such as serology, bacterial isolation, and molecular analysis in buffaloes bred in Maranhão State. In order to do so, 390 samples of buffalo serum were subjected to serological tests, to Rose Bengal Plate Test (RBPT) and to 2-mercaptoethanol (2-ME) combined with slow agglutination test (SAT). Vaginal swabs were collected from seropositive animals and subjected to bacterial isolation and to generic PCR. According to the serological test, 16 animals had a positive reaction to the confirmatory test (2-ME/SAT). As for bacterial isolation, three samples resulted in the isolation of Brucella spp.-characteristic colonies, which were confirmed through PCR. These results confirmed Brucella spp. infection in the buffalo herd from Maranhão State.
[Mh] Termos MeSH primário: Brucella/isolamento & purificação
Brucelose/veterinária
Búfalos/microbiologia
[Mh] Termos MeSH secundário: Testes de Aglutinação/veterinária
Animais
Anticorpos Antibacterianos/sangue
Brasil
Brucelose/sangue
Brucelose/diagnóstico
Feminino
Mercaptoetanol
Reação em Cadeia da Polimerase
Rosa Bengala
Testes Sorológicos/métodos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Bacterial); 1ZPG1ELY14 (Rose Bengal); 60-24-2 (Mercaptoethanol)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:171102
[Lr] Data última revisão:
171102
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170220
[St] Status:MEDLINE
[do] DOI:10.1007/s11250-017-1238-3


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[PMID]:27830465
[Au] Autor:Moreno-Hernández JM; Hernández-Mancillas XD; Navarrete ELC; Mazorra-Manzano MÁ; Osuna-Ruiz I; Rodríguez-Tirado VA; Salazar-Leyva JA
[Ad] Endereço:Programa de Investigación en Biotecnología. Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias (INIFAP), Campo Experimental Valle de Culiacán, Km. 17.5 Carretera Culiacán-Eldorado, 80000, Culiacán, SIN, Mexico.
[Ti] Título:Partial Characterization of the Proteolytic Properties of an Enzymatic Extract From "Aguama" Bromelia pinguin L. Fruit Grown in Mexico.
[So] Source:Appl Biochem Biotechnol;182(1):181-196, 2017 May.
[Is] ISSN:1559-0291
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Plant proteases are capable of performing several functions in biological systems, and their use is attractive for biotechnological process due to their interesting catalytic properties. Bromelia pinguin (aguama) is a wild abundant natural resource in several regions of Central America and the Caribbean Islands but is underutilized. Their fruits are rich in proteases with properties that are still unknown, but they represent an attractive source of enzymes for biotechnological applications. Thus, the proteolytic activity in enzymatic crude extracts (CEs) from wild B. pinguin fruits was partially characterized. Enzymes in CEs showed high proteolytic activity at acid (pH 2.0-4.0) and neutral alkaline (pH 7.0-9.0) conditions, indicating that different types of active proteases are present. Proteolytic activity inhibition by the use of specific protease inhibitors indicated that aspartic, cysteine, and serine proteases are the main types of proteases present in CEs. Activity at pH 3.0 was stable in a broad range of temperatures (25-50 °C) and retained its activity in the presence of surfactants (SDS, Tween-80), reducing agents (DTT, 2-mercapoethanol), and organic solvents (methanol, ethanol, acetone, 2-propanol), which suggests that B. pinguin proteases are potential candidates for their application in brewing, detergent, and pharmaceutical industries.
[Mh] Termos MeSH primário: Ácido Aspártico Proteases/química
Bromelia/enzimologia
Cisteína Proteases/química
Frutas/enzimologia
Proteínas de Plantas/química
Serina Proteases/química
[Mh] Termos MeSH secundário: Ácido Aspártico Proteases/antagonistas & inibidores
Ácido Aspártico Proteases/isolamento & purificação
Bromelia/química
Cisteína Proteases/isolamento & purificação
Ditiotreitol/química
Ensaios Enzimáticos
Frutas/química
Concentração de Íons de Hidrogênio
Cinética
Mercaptoetanol/química
Extratos Vegetais/química
Proteínas de Plantas/antagonistas & inibidores
Proteínas de Plantas/isolamento & purificação
Polissorbatos/química
Inibidores de Proteases/química
Proteólise
Serina Proteases/isolamento & purificação
Dodecilsulfato de Sódio/química
Solventes/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Plant Extracts); 0 (Plant Proteins); 0 (Polysorbates); 0 (Protease Inhibitors); 0 (Solvents); 368GB5141J (Sodium Dodecyl Sulfate); 60-24-2 (Mercaptoethanol); EC 3.4.- (Aspartic Acid Proteases); EC 3.4.- (Cysteine Proteases); EC 3.4.- (Serine Proteases); T8ID5YZU6Y (Dithiothreitol)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170619
[Lr] Data última revisão:
170619
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161111
[St] Status:MEDLINE
[do] DOI:10.1007/s12010-016-2319-x


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[PMID]:27293035
[Au] Autor:Salihi A; Asoodeh A; Aliabadian M
[Ad] Endereço:Department of Chemistry, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran.
[Ti] Título:Production and biochemical characterization of an alkaline protease from Aspergillus oryzae CH93.
[So] Source:Int J Biol Macromol;94(Pt B):827-835, 2017 Jan.
[Is] ISSN:1879-0003
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:In this study, Aspergillus oryzae CH93 was isolated from soil sample and examined using molecular analysis. Following culture of A. oryzae CH93 under optimal enzyme production, a 47.5kDa extracellular protease was purified using ammonium sulfate precipitation and Q-Sepharose chromatography. The optimal pH 8 and temperature of 50°C obtained for the isolated protease. Sodium dodecyl sulfate (SDS), cetyltrimethyl ammonium bromide (CTAB), H O decreased activity, while Triton X-100 and phenylmethanesulfonyl fluoride (PMSF) had no inhibitory effect on the enzyme activity; meanwhile, 2-mercaptoethanol and ethylenediaminetetraacetic acid (EDTA) declined the protease activity. Isoamyl alcohol and acetone (30%) enhanced activity whereas 2-propanol, isopropanol and dimethyl sulfoxide (DMSO) (30%) reduced protease activity. The enzyme exhibited a half-life of 100min at its optimum temperature. Among five substrates of bovine serum albumin (BSA), N-acetyl-l-tyrosine ethyl ester monohydrate (ATEE), casein, azocasein and gelatin results showed that casein is the best substrate with V of 0.1411±0.004µg/min and K of 2.432±0.266µg/ml. In conclusion, the extracted protease from A. oryzae CH93 as a fungal source possessed biochemical features which could be useful in some application usages.
[Mh] Termos MeSH primário: Aspergillus oryzae/enzimologia
Proteínas de Bactérias/química
Endopeptidases/química
Proteínas Fúngicas/química
Inibidores de Proteases/química
Microbiologia do Solo
[Mh] Termos MeSH secundário: 2-Propanol/química
Aspergillus oryzae/química
Aspergillus oryzae/classificação
Proteínas de Bactérias/biossíntese
Proteínas de Bactérias/isolamento & purificação
Caseínas/química
Dimetil Sulfóxido/química
Ácido Edético/química
Endopeptidases/biossíntese
Endopeptidases/isolamento & purificação
Estabilidade Enzimática
Proteínas Fúngicas/biossíntese
Proteínas Fúngicas/isolamento & purificação
Meia-Vida
Concentração de Íons de Hidrogênio
Mercaptoetanol/química
Filogenia
Especificidade por Substrato
Temperatura Ambiente
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Caseins); 0 (Fungal Proteins); 0 (Protease Inhibitors); 60-24-2 (Mercaptoethanol); 9G34HU7RV0 (Edetic Acid); EC 3.4.- (Endopeptidases); EC 3.4.99.- (alkaline protease); ND2M416302 (2-Propanol); YOW8V9698H (Dimethyl Sulfoxide)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170320
[Lr] Data última revisão:
170320
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160614
[St] Status:MEDLINE


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[PMID]:27840405
[Au] Autor:Shi Y; Hu Y; Lv C; Tu G
[Ad] Endereço:Department of Orthopaedic Surgery, The First Affiliated Hospital of China Medical University, Shenyang, Liaoning, China (mainland).
[Ti] Título:Effects of Reactive Oxygen Species on Differentiation of Bone Marrow Mesenchymal Stem Cells.
[So] Source:Ann Transplant;21:695-700, 2016 Nov 14.
[Is] ISSN:2329-0358
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND The low differentiation rates for transplanted stem cells are challenging problems in spinal cord injury (SCI) treatment. Studies have demonstrated that the inhibition of the Notch1 pathway in bone marrow mesenchymal stem cells (BMSCs) contributed to the differentiation of these cells. Research findings that certain antioxidants induce BMSCs to differentiate into neuronal cells suggest that BMSC differentiation is related to the level of reactive oxygen species (ROS) in cells. This study aimed to define the effect of ROS on the differentiation of BMSCs. MATERIAL AND METHODS In this study, after BMSCs were induced with the antioxidant ß-mercaptoethanol (ß-ME), related proteins were analyzed by Western blotting and immunofluorescence. In order to find the role of ROS in the differentiation, DCFH-DA was used to detect ROS levels in antioxidant-treated BMSCs, H2O2-treated BMSCs, and normal BMSCs, and the expression levels of Notch1 and its downstream transcriptional suppressor Hes1 were analyzed. RESULTS Induced with ß-ME, Western blotting and immunofluorescence revealed gradual increases in the expression of Nestin (a neural stem cell-specific protein) and neuron-specific enolase (NSE) but decreases in Notch1 expression. The expression levels of Notch1 and Hes1 were positively correlated with changes in ROS level. CONCLUSIONS These data suggest that the antioxidant-induced differentiation of BMSCs into neurons may be related to ROS-based regulation of the Notch1 signalling pathway.
[Mh] Termos MeSH primário: Células da Medula Óssea/citologia
Diferenciação Celular/fisiologia
Células Mesenquimais Estromais/citologia
Espécies Reativas de Oxigênio/metabolismo
Transdução de Sinais/fisiologia
[Mh] Termos MeSH secundário: Animais
Antioxidantes/farmacologia
Células da Medula Óssea/efeitos dos fármacos
Células da Medula Óssea/metabolismo
Diferenciação Celular/efeitos dos fármacos
Linhagem Celular
Sobrevivência Celular/efeitos dos fármacos
Sobrevivência Celular/fisiologia
Mercaptoetanol/farmacologia
Células Mesenquimais Estromais/efeitos dos fármacos
Células Mesenquimais Estromais/metabolismo
Fosfopiruvato Hidratase/metabolismo
Ratos
Receptor Notch1/metabolismo
Transdução de Sinais/efeitos dos fármacos
Fatores de Transcrição HES-1/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antioxidants); 0 (Hes1 protein, rat); 0 (Reactive Oxygen Species); 0 (Receptor, Notch1); 0 (Transcription Factor HES-1); 60-24-2 (Mercaptoethanol); EC 4.2.1.11 (Phosphopyruvate Hydratase)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161115
[St] Status:MEDLINE


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[PMID]:27650672
[Au] Autor:Merlo B; Iacono E; Bucci D; Spinaci M; Galeati G; Mari G
[Ad] Endereço:Department of Veterinary Medical Sciences, University of Bologna, Bologna, Ozzano Emilia, Italy.
[Ti] Título:Beta-mercaptoethanol supplementation of in vitro maturation medium does not influence nuclear and cytoplasmic maturation of equine oocytes.
[So] Source:Reprod Domest Anim;51(6):992-996, 2016 Dec.
[Is] ISSN:1439-0531
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:In vitro embryo production in the horse is still not as efficient as in other species. Oxidative stress negatively affects oocyte and embryo culture. To attenuate/minimize the oxidative stress, antioxidants such as low-molecular thiol compounds can be added to culture media. Beta-mercaptoethanol (BME) has been shown to improve maturation and embryo development in different species. The aim of this study was to investigate whether the addition to maturation medium of BME at common (0.1 mM) and high (0.7 mM) concentration could improve oocyte maturation also in the horse. Equine oocytes recovered from slaughterhouse ovaries were used. Meiotic configuration after in vitro maturation (IVM) and early embryo production after intracytoplasmic sperm injection (ICSI) were considered as criteria for assessing nuclear and cytoplasmic maturation, respectively. A total of 1,076 oocytes were analysed over two experiments: 848 (control n = 293, BME 0.1 n = 270, BME 0.7 n = 285) were stained with Hoechst 33342 and examined for nuclear stage after 26 hr of IVM, and 228 MII oocytes were fertilized by ICSI (control n = 83, BME 0.1 n = 65, BME 0.7 n = 80). Cleavage rates were determined after 60 hr of culture. Unlike results obtained in other species, the addition of BME did not influence maturation rates (51.9% control vs 55.6% BME 0.1 mM and 55.1% BME 0.7 mM), nor cleavage rates after ICSI (38.6% vs 38.5% and 41.3%, respectively). In conclusion, the addition of BME at 0.1 and 0.7 mM to the maturation medium, in our culture conditions, has no effect on nuclear and cytoplasmic maturation of equine oocytes.
[Mh] Termos MeSH primário: Cavalos/fisiologia
Técnicas de Maturação in Vitro de Oócitos/veterinária
Mercaptoetanol/farmacologia
Oócitos/fisiologia
[Mh] Termos MeSH secundário: Animais
Núcleo Celular/fisiologia
Meios de Cultura
Técnicas de Cultura Embrionária
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Culture Media); 60-24-2 (Mercaptoethanol)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170406
[Lr] Data última revisão:
170406
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160922
[St] Status:MEDLINE
[do] DOI:10.1111/rda.12778


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[PMID]:27374230
[Au] Autor:Alba A; Sánchez J; Hernández H; Mosqueda M; Rodríguez SY; Capó V; Otero O; Alfonso C; Marcet R; Sarracent J
[Ad] Endereço:Vicedirección de Investigaciones en Parasitología, Instituto de Medicina Tropical "Pedro Kourí", La Habana, Cuba. Electronic address: annia@ipk.sld.cu.
[Ti] Título:Insights into the biological features of the antigenic determinants recognized by four monoclonal antibodies in redia and adult stages of the liver fluke Fasciola hepatica.
[So] Source:Exp Parasitol;168:39-44, 2016 Sep.
[Is] ISSN:1090-2449
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Fasciola hepatica is a digenean trematode which infects a wide variety of domestic animals and also humans. Previous studies have demonstrated that four monoclonal antibodies (Mabs) against the total extract of F. hepatica redia (named as 1E4, 6G11, 4E5 and 4G11) also recognized the excretion - secretion antigens (ES Ag) of adult parasites, which is a biologically-relevant mixture of molecules with functional roles during infection and immune evasion on definitive hosts. In the present report we describe the partial characterization of the epitopes recognized by these Mabs by heat treatment, mercaptoethanol reduction, pronase proteolysis and sodium peryodate oxidation, which suggested their predominant protein and conformational nature. Also, a comparative study using immunodetection assays on crude extracts and on histological sections of both rediae and adults of F. hepatica were performed to explore the expression pattern of the antigenic determinants in these developmental stages. From these experiments it was found that the Mabs reacted most likely with the same proteins of approximately 64 and 105 kDa present on both rediae and adult's extracts. However, the 1E4, 6G11 and 4E5 Mabs also recognized other molecules of the total extract of F. hepatica adults, a fact that constitutes an evidence of the antigenic variation between both stages and points at a certain biological relevance of the recognized antigenic determinants. Immunolocalization studies on histological sections revealed that all Mabs reacted with the tegument of F. hepatica in both rediae and adults stages, while the epitopes recognized by 1E4, 6G11 and 4E5 antibodies were also preferentially localized in the intestinal caeca and in different organs of the reproductive system of adult specimens. The immunogenicity of these antigenic determinants, their conserved status among different stages of the life cycle of F. hepatica and their presence in both tegument and ES Ag of adult parasites, are suitable features that suggest their potential use for developing an epitope-based vaccine for fasciolosis control.
[Mh] Termos MeSH primário: Anticorpos Monoclonais/imunologia
Epitopos/imunologia
Fasciola hepatica/imunologia
[Mh] Termos MeSH secundário: Animais
Variação Antigênica/fisiologia
Epitopos/química
Epitopos/metabolismo
Fasciola hepatica/efeitos dos fármacos
Imuno-Histoquímica
Mercaptoetanol/farmacologia
Camundongos
Oxirredução
Ácido Periódico/farmacologia
Pronase/metabolismo
Temperatura Ambiente
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Monoclonal); 0 (Epitopes); 10450-60-9 (Periodic Acid); 60-24-2 (Mercaptoethanol); B45A1BUM4Q (metaperiodate); EC 3.4.24.- (Pronase)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170106
[Lr] Data última revisão:
170106
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160705
[St] Status:MEDLINE


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[PMID]:27287154
[Au] Autor:Khan S; Wang Z; Wang R; Zhang L
[Ad] Endereço:State Key Laboratory of Organic-Inorganic Composites and Key Laboratory of Beijing City for Preparation and Processing of Novel Polymer Materials, Beijing University of Chemical Technology, 100029 Beijing, PR China; Institute of Chemical Sciences, Gomal University, Dera Ismail Khan, KPK, Pakistan.
[Ti] Título:Synthesis and structure design of new bio-based elastomers via Thiol-ene-Click Reactions.
[So] Source:Mater Sci Eng C Mater Biol Appl;67:554-560, 2016 Oct 01.
[Is] ISSN:1873-0191
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The additions of 2-mercaptoethanol to (S)-(-)-limonene via click reaction is described as an adaptable and efficient way to obtain alcohol functionalized renewable monomer for the synthesis of new cross-linkable bio-based elastomers. Thiol first reacted with the limonene endocyclic double bond and then reacted with the exocyclics double bond to form the difunctional monomer. The structure of the monomer was determined by using FTIR, (1)H NMR and mass spectrometry. Thermal Gravimetric Analysis (TGA) and Differential Scanning Calorimetrys (DSC) characterization exposed that this monomer could be used to synthesize elastomers with excellent and adaptable thermal properties. The molecular weight of the synthesized elastomer could reach 186kDaa via melting polycondensation route and the structure-properties relationship was deliberated. Finally, these elastomers were mixed with dicumyl peroxide (DCP) to form cross-linked elastomers with certain mechanical property, and the gel contents of the elastomers were confirmed by using Soxhlet extraction method.
[Mh] Termos MeSH primário: Química Click/métodos
Cicloexenos/química
Elastômeros
Mercaptoetanol/química
Terpenos/química
[Mh] Termos MeSH secundário: Compostos de Benzil/química
Reagentes para Ligações Cruzadas/química
Elastômeros/síntese química
Elastômeros/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Benzyl Compounds); 0 (Cross-Linking Reagents); 0 (Cyclohexenes); 0 (Elastomers); 0 (Terpenes); 60-24-2 (Mercaptoethanol); 9MC3I34447 (limonene); M51X2J0U9D (dicumyl peroxide)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170812
[Lr] Data última revisão:
170812
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160612
[St] Status:MEDLINE


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[PMID]:27166317
[Au] Autor:Deighan WI; O'Kane MJ; McNicholl FP; Keren DF
[Ad] Endereço:1 Department of Clinical Chemistry, Altnagelvin Hospital, Londonderry, N. Ireland.
[Ti] Título:Multiple myeloma and multiple plasmacytomas associated with free gamma heavy chain, free kappa light chain and IgGk paraproteins: an unusual triple gammopathy.
[So] Source:Ann Clin Biochem;53(6):706-711, 2016 Nov.
[Is] ISSN:1758-1001
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Multiple myeloma is a malignant plasma cell dyscrasia that is becoming more prevalent in an increasingly ageing population. It is a complex disease with clinical phases ranging from the premalignant monoclonal gammopathy of undetermined significance to asymptomatic (smouldering) myeloma and then symptomatic myeloma; the latter occasionally terminating in the clonal proliferation of plasma cells outside the bone marrow. We present a patient whose clonally evolved disease from monoclonal gammopathy of undetermined significance to multiple myeloma demonstrated the presence of an unusual combination of monoclonal immunoproteins. Capillary electrophoresis demonstrated the presence of three paraproteins in the gamma region (γ-region), two of which were additional to the IgGk paraprotein which migrated in the slow γ-region at initial diagnosis. Subsequent isotypic identification of the new paraproteins was not possible by immunotyping and initial immunofixation studies failed to definitively characterize the monoclonal proteins. After reduction with beta-mercaptoethanol, two paraproteins were detected by both capillary and gel electrophoresis. However, only immunofixation was able to resolve three distinct monoclonal bands, confirming the presence of free monoclonal kappa light chains in the mid-gamma region and free monoclonal heavy chains in the fast gamma region. Triple gammopathies in themselves are uncommon; this case presents a very unusual combination of paraproteins which required various electrophoretical and immunochemical techniques to identify and characterize them. The change of electrophoretic signature from the monoclonal gammopathy of undetermined significance phase to the diagnosis of multiple myeloma suggested that a number of genetically distinct subclones were present in the pretreatment clonal evolution of the disease.
[Mh] Termos MeSH primário: Cadeias kappa de Imunoglobulina/isolamento & purificação
Gamopatia Monoclonal de Significância Indeterminada/diagnóstico
Mieloma Múltiplo/diagnóstico
Proteínas do Mieloma/isolamento & purificação
Paraproteínas/isolamento & purificação
Plasmocitoma/diagnóstico
[Mh] Termos MeSH secundário: Idoso
Evolução Clonal
Progressão da Doença
Eletroforese Capilar
Eletroforese em Gel de Poliacrilamida
Feminino
Seres Humanos
Cadeias kappa de Imunoglobulina/imunologia
Mercaptoetanol/química
Gamopatia Monoclonal de Significância Indeterminada/imunologia
Gamopatia Monoclonal de Significância Indeterminada/patologia
Mieloma Múltiplo/imunologia
Mieloma Múltiplo/patologia
Proteínas do Mieloma/imunologia
Paraproteínas/imunologia
Plasmocitoma/imunologia
Plasmocitoma/patologia
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Immunoglobulin kappa-Chains); 0 (Myeloma Proteins); 0 (Paraproteins); 0 (multiple myeloma M-proteins); 60-24-2 (Mercaptoethanol)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160512
[St] Status:MEDLINE
[do] DOI:10.1177/0004563216646594


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[PMID]:27107836
[Au] Autor:Caponi L; Franzini M; Koni E; Masotti S; Petrini M; Paolicchi A
[Ti] Título:Discrepancy between FLC assays: only a problem of quantification?
[So] Source:Clin Chem Lab Med;54(6):1111-3, 2016 Jun 01.
[Is] ISSN:1437-4331
[Cp] País de publicação:Germany
[La] Idioma:eng
[Mh] Termos MeSH primário: Imunoensaio/métodos
Cadeias Leves de Imunoglobulina/sangue
[Mh] Termos MeSH secundário: Cromatografia em Gel
Seres Humanos
Cadeias Leves de Imunoglobulina/isolamento & purificação
Mercaptoetanol/química
Mieloma Múltiplo/sangue
Oxirredução
Estrutura Quaternária de Proteína
[Pt] Tipo de publicação:LETTER
[Nm] Nome de substância:
0 (Immunoglobulin Light Chains); 60-24-2 (Mercaptoethanol)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160425
[St] Status:MEDLINE



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