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Pesquisa : D02.033.375.650 [Categoria DeCS]
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  1 / 1871 MEDLINE  
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[PMID]:29037702
[Au] Autor:Benbettaïeb N; Tanner C; Cayot P; Karbowiak T; Debeaufort F
[Ad] Endereço:Univ. Bourgogne Franche-Comté/Agrosup Dijon, UMR PAM A02-102, Food and Wine Physical-Chemistry Lab, 1 esplanade Erasme, 21000 Dijon, France; IUT-Dijon-Auxerre, Dpt BioEngineering, 7 blvd Docteur Petitjean, 20178 Dijon Cedex, France.
[Ti] Título:Impact of functional properties and release kinetics on antioxidant activity of biopolymer active films and coatings.
[So] Source:Food Chem;242:369-377, 2018 Mar 01.
[Is] ISSN:0308-8146
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:This work deals with the study of the release kinetics of some natural antioxidants (ferulic acid, caffeic acid and tyrosol) from chitosan-fish gelatin edible films immersed ethanol at 96%, as well as the kinetics of their antioxidant activity using the DPPH assay. The aim was to determine how film functional properties influence the release kinetic and antioxidant activity. The addition of antioxidants to chitosan-fish gelatin matrix decreased the water vapour permeability by more than 30%. The tensile strength (TS) increased up to 50% after the incorporation of antioxidants. Some molecular interactions between polymer chains and antioxidants were confirmed by FTIR where spectra displayed a shift of the amide-III peak. Films containing caffeic acid or a caffeic-ferulic acid mixture exhibited the highest radical scavenging activity, leading to a 90% antioxidant activity at equilibrium but the release rate controlled the efficacy of the system.
[Mh] Termos MeSH primário: Antioxidantes/análise
Biopolímeros/química
Quitosana/química
Gelatina/química
[Mh] Termos MeSH secundário: Antioxidantes/química
Ácidos Cafeicos/análise
Ácidos Cafeicos/química
Ácidos Cumáricos/análise
Ácidos Cumáricos/química
Produtos Pesqueiros
Cinética
Permeabilidade
Álcool Feniletílico/análogos & derivados
Álcool Feniletílico/análise
Álcool Feniletílico/química
Resistência à Tração
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antioxidants); 0 (Biopolymers); 0 (Caffeic Acids); 0 (Coumaric Acids); 1AK4MU3SNX (4-hydroxyphenylethanol); 9000-70-8 (Gelatin); 9012-76-4 (Chitosan); AVM951ZWST (ferulic acid); ML9LGA7468 (Phenylethyl Alcohol); U2S3A33KVM (caffeic acid)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180222
[Lr] Data última revisão:
180222
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171018
[St] Status:MEDLINE


  2 / 1871 MEDLINE  
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[PMID]:29037699
[Au] Autor:Álvarez-Fernández MA; Fernández-Cruz E; Cantos-Villar E; Troncoso AM; García-Parrilla MC
[Ad] Endereço:Departamento de Nutrición y Bromatología, Toxicología y Medicina Legal, Facultad de Farmacia, Universidad de Sevilla, C/ P, García González n° 2, Sevilla 41012, Spain.
[Ti] Título:Determination of hydroxytyrosol produced by winemaking yeasts during alcoholic fermentation using a validated UHPLC-HRMS method.
[So] Source:Food Chem;242:345-351, 2018 Mar 01.
[Is] ISSN:0308-8146
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Hydroxytyrosol (HT) is a phenolic compound of recognized bioactivity that has been described in wines but little is known about its origin. This work demonstrates that yeast involved in wine making, i.e. Saccharomyces cerevisiae strains and the non-Saccharomyces Torulaspora delbrueckii, can synthesise HT, as this compound was identified in the intracellular media of three strains by means of a developed and validated UHPLC-HRMS method with LOQ and LOD of 0.108 and 0.035ngmL respectively. Controlled fermentations were performed with different varieties of grapes (Corredera, Moscatel, Chardonnay, Palomino fino, Sauvignon Blanc, Vijiriega, and Tempranillo) and synthetic must. The Saccharomyces cerevisiae strain QA23 was the most efficient producer of HT from tested yeasts. On the other hand, the grape variety influences HT wine concentrations. Furthermore, the maximum concentration of HT is reached between the fourth and sixth day of fermentation. This work reveals that yeasts have a great potential for the production of HT.
[Mh] Termos MeSH primário: Fermentação
Álcool Feniletílico/análogos & derivados
Saccharomyces cerevisiae/metabolismo
Torulaspora/metabolismo
Vinho/análise
[Mh] Termos MeSH secundário: Cromatografia Líquida de Alta Pressão
Álcool Feniletílico/análise
Álcool Feniletílico/metabolismo
Vitis
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
10597-60-1 (3,4-dihydroxyphenylethanol); ML9LGA7468 (Phenylethyl Alcohol)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180222
[Lr] Data última revisão:
180222
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171018
[St] Status:MEDLINE


  3 / 1871 MEDLINE  
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[PMID]:29374707
[Au] Autor:Uesawa Y; Sakagami H; Okudaira N; Toda K; Takao K; Kagaya H; Sugita Y
[Ad] Endereço:Department of Clinical Pharmaceutics, Meiji Pharmaceutical University, Tokyo, Japan uesawa@my-pharm.ac.jp.
[Ti] Título:Quantitative Structure-Cytotoxicity Relationship of Cinnamic Acid Phenetyl Esters.
[So] Source:Anticancer Res;38(2):817-823, 2018 02.
[Is] ISSN:1791-7530
[Cp] País de publicação:Greece
[La] Idioma:eng
[Ab] Resumo:BACKGROUND/AIM: Many phenolic acid phenethyl esters possess diverse biological effects including antioxidant, cytoprotective, anti-inflammation and anti-tumor activities. However, most previous antitumor studies have not considered the cytotoxicity against normal cells. Ten cinnamic acid phenetyl esters were subjected to quantitative structure-activity relationship (QSAR) analysis, based on their cytotoxicity and tumor-specificity, in order to find their new biological activities. MATERIALS AND METHODS: Cytotoxicity against four human oral squamous cell carcinoma cell lines and three oral normal mesenchymal cells was determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) method. Tumor specificity (TS) was evaluated by the ratio of the mean 50% cytotoxic concentration (CC ) against normal oral cells to that against human oral squamous cell carcinoma cell lines. Potency-selectivity expression (PSE) value was calculated by dividing the TS value by CC against tumor cells. Apoptosis markers were detected by western blot analysis. Physicochemical, structural and quantum-chemical parameters were calculated based on the conformations optimized by force-field minimization. RESULTS: Western blot analysis demonstrated that [ ] stimulated the cleavage of caspase-3, suggesting the induction of apoptosis. QSAR analysis demonstrated that TS values were correlated with shape, size and ionization potential. CONCLUSION: Chemical modification of the lead compound may be a potential choice for designing a new type of anticancer drugs.
[Mh] Termos MeSH primário: Cinamatos/farmacologia
Ésteres/farmacologia
Álcool Feniletílico/farmacologia
[Mh] Termos MeSH secundário: Carcinoma de Células Escamosas/tratamento farmacológico
Carcinoma de Células Escamosas/patologia
Linhagem Celular Tumoral
Criança
Cinamatos/química
Cinamatos/toxicidade
Ésteres/química
Ésteres/toxicidade
Fibroblastos/efeitos dos fármacos
Neoplasias de Cabeça e Pescoço/tratamento farmacológico
Neoplasias de Cabeça e Pescoço/patologia
Seres Humanos
Masculino
Neoplasias Bucais/tratamento farmacológico
Neoplasias Bucais/patologia
Álcool Feniletílico/química
Álcool Feniletílico/toxicidade
Relação Quantitativa Estrutura-Atividade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cinnamates); 0 (Esters); ML9LGA7468 (Phenylethyl Alcohol); U14A832J8D (cinnamic acid)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180221
[Lr] Data última revisão:
180221
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180129
[St] Status:MEDLINE


  4 / 1871 MEDLINE  
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[PMID]:29278909
[Au] Autor:González-Correa JA; Rodríguez-Pérez MD; Márquez-Estrada L; López-Villodres JA; Reyes JJ; Rodriguez-Gutierrez G; Fernández-Bolaños J; De La Cruz JP
[Ad] Endereço:Departmento de Farmacología, Facultad de Medicina, Instituto de Investigación Biomédica (IBIMA), Universidad de Málaga , 29016 Málaga, Spain.
[Ti] Título:Neuroprotective Effect of Hydroxytyrosol in Experimental Diabetic Retinopathy: Relationship with Cardiovascular Biomarkers.
[So] Source:J Agric Food Chem;66(3):637-644, 2018 Jan 24.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The aim of the study was to test the neuroprotective effect of hydroxytyrosol (HT) on experimental diabetic retinopathy. Animals were divided in four groups: (1) control nondiabetic rats, (2) streptozotocin-diabetic rats (DR), (3) DR treated with 1 mg/kg/day p.o. HT, and (4) DR treated with 5 mg/kg/day p.o. HT. Treatment with HT was started 7 days before inducing diabetes and was maintained for 2 months. In the DR group, total area occupied by extracellular matrix was increased, area occupied by retinal cells was decreased; both returned to near-control values in DR rats treated with HT. The number of retinal ganglion cells in DR was significantly lower (44%) than in the control group, and this decrease was smaller after HT treatment (34% and 9.1%). Linear regression analysis showed that prostacyclin, platelet aggregation, peroxynitrites, and the dose of 5 mg/kg/day HT significantly influenced retinal ganglion cell count. In conclusion, HT exerted a neuroprotective effect on diabetic retinopathy, and this effect correlated significantly with changes in some cardiovascular biomarkers.
[Mh] Termos MeSH primário: Sistema Cardiovascular/efeitos dos fármacos
Diabetes Mellitus Tipo 1/complicações
Retinopatia Diabética/prevenção & controle
Fármacos Neuroprotetores/administração & dosagem
Álcool Feniletílico/análogos & derivados
Extratos Vegetais/administração & dosagem
[Mh] Termos MeSH secundário: Animais
Biomarcadores/sangue
Sistema Cardiovascular/metabolismo
Retinopatia Diabética/sangue
Retinopatia Diabética/etiologia
Retinopatia Diabética/fisiopatologia
Seres Humanos
Olea/química
Álcool Feniletílico/administração & dosagem
Álcool Feniletílico/química
Extratos Vegetais/química
Agregação Plaquetária/efeitos dos fármacos
Ratos
Ratos Wistar
Retina/efeitos dos fármacos
Retina/metabolismo
Células Ganglionares da Retina/citologia
Células Ganglionares da Retina/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biomarkers); 0 (Neuroprotective Agents); 0 (Plant Extracts); 10597-60-1 (3,4-dihydroxyphenylethanol); ML9LGA7468 (Phenylethyl Alcohol)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180207
[Lr] Data última revisão:
180207
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171228
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jafc.7b05063


  5 / 1871 MEDLINE  
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[PMID]:28452954
[Au] Autor:Echeverría F; Ortiz M; Valenzuela R; Videla LA
[Ad] Endereço:Nutrition Department, Faculty of Medicine, University of Chile, Independencia 1027, Independencia, Santiago 8380453, Chile. francisca.echeverria@med.uchile.cl.
[Ti] Título:Hydroxytyrosol and Cytoprotection: A Projection for Clinical Interventions.
[So] Source:Int J Mol Sci;18(5), 2017 Apr 28.
[Is] ISSN:1422-0067
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:Hydroxytyrosol (HT) ((3,4-Dihydroxyphenyl)ethanol) is a polyphenol mainly present in extra virgin olive oil (EVOO) but also in red wine. It has a potent antioxidant effect related to hydrogen donation, and the ability to improve radical stability. The phenolic content of olive oil varies between 100 and 600 mg/kg, due to multiple factors (place of cultivation, climate, variety of the olive and level of ripening at the time of harvest), with HT and its derivatives providing half of that content. When consumed, EVOO's phenolic compounds are hydrolyzed in the stomach and intestine, increasing levels of free HT which is then absorbed in the small intestine, forming phase II metabolites. It has been demonstrated that HT consumption is safe even at high doses, and that is not genotoxic or mutagenic in vitro. The beneficial effects of HT have been studied in humans, as well as cellular and animal models, mostly in relation to consumption of EVOO. Many properties, besides its antioxidant capacity, have been attributed to this polyphenol. The aim of this review was to assess the main properties of HT for human health with emphasis on those related to the possible prevention and/or treatment of non-communicable diseases.
[Mh] Termos MeSH primário: Citoproteção/efeitos dos fármacos
Álcool Feniletílico/análogos & derivados
[Mh] Termos MeSH secundário: Animais
Anti-Inflamatórios/química
Anti-Inflamatórios/farmacologia
Antineoplásicos Fitogênicos/química
Antineoplásicos Fitogênicos/farmacologia
Antioxidantes/química
Antioxidantes/farmacologia
Autofagia/efeitos dos fármacos
Seres Humanos
Mitocôndrias/efeitos dos fármacos
Mitocôndrias/metabolismo
Azeite de Oliva/química
Estresse Oxidativo/efeitos dos fármacos
Álcool Feniletílico/química
Álcool Feniletílico/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Anti-Inflammatory Agents); 0 (Antineoplastic Agents, Phytogenic); 0 (Antioxidants); 0 (Olive Oil); 10597-60-1 (3,4-dihydroxyphenylethanol); ML9LGA7468 (Phenylethyl Alcohol)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180122
[Lr] Data última revisão:
180122
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170429
[St] Status:MEDLINE


  6 / 1871 MEDLINE  
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[PMID]:29202400
[Au] Autor:Chen L; Jin Y; Chen H; Sun C; Fu W; Zheng L; Lu M; Chen P; Chen G; Zhang Y; Liu Z; Wang Y; Song Z; Liang G
[Ad] Endereço:Chemical Biology Research Center, School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou, Zhejiang, 325035, China; School of Chemical Engineering, Nanjing University of Science and Technology, Nanjing, Jiangsu, 210094, China.
[Ti] Título:Discovery of caffeic acid phenethyl ester derivatives as novel myeloid differentiation protein 2 inhibitors for treatment of acute lung injury.
[So] Source:Eur J Med Chem;143:361-375, 2018 Jan 01.
[Is] ISSN:1768-3254
[Cp] País de publicação:France
[La] Idioma:eng
[Ab] Resumo:Myeloid differentiation protein 2 (MD2) is an essential molecule which recognizes lipopolysaccharide (LPS), leading to initiation of inflammation through the activation of Toll-like receptor 4 (TLR4) signaling. Caffeic acid phenethyl ester (CAPE) from propolis of honeybee hives could interfere interactions between LPS and the TLR4/MD2 complex, and thereby has promising anti-inflammatory properties. In this study, we designed and synthesized 48 CAPE derivatives and evaluated their anti-inflammatory activities in mouse primary peritoneal macrophages (MPMs) activated by LPS. The most active compound, 10s, was found to bind with MD2 with high affinity, which prevented formation of the LPS/MD2/TLR4 complex. The binding mode of 10s revealed that the major interactions with MD2 were established via two key hydrogen bonds and hydrophobic interactions. Furthermore, 10s showed remarkable protective effects against LPS-caused ALI (acute lung injury) in vivo. Taken together, this work provides new lead structures and candidates as MD2 inhibitors for the development of anti-inflammatory drugs.
[Mh] Termos MeSH primário: Lesão Pulmonar Aguda/tratamento farmacológico
Anti-Inflamatórios não Esteroides/farmacologia
Ácidos Cafeicos/farmacologia
Descoberta de Drogas
Antígeno 96 de Linfócito/antagonistas & inibidores
Álcool Feniletílico/análogos & derivados
[Mh] Termos MeSH secundário: Lesão Pulmonar Aguda/metabolismo
Animais
Anti-Inflamatórios não Esteroides/síntese química
Anti-Inflamatórios não Esteroides/química
Ácidos Cafeicos/síntese química
Ácidos Cafeicos/química
Citocinas/antagonistas & inibidores
Citocinas/biossíntese
Relação Dose-Resposta a Droga
Seres Humanos
Inflamação/tratamento farmacológico
Inflamação/metabolismo
Lipopolissacarídeos/antagonistas & inibidores
Lipopolissacarídeos/farmacologia
Antígeno 96 de Linfócito/metabolismo
Macrófagos/efeitos dos fármacos
Macrófagos/metabolismo
Camundongos
Camundongos Endogâmicos C57BL
Estrutura Molecular
Álcool Feniletílico/síntese química
Álcool Feniletílico/química
Álcool Feniletílico/farmacologia
Relação Estrutura-Atividade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Inflammatory Agents, Non-Steroidal); 0 (Caffeic Acids); 0 (Cytokines); 0 (LY96 protein, human); 0 (Lipopolysaccharides); 0 (Lymphocyte Antigen 96); G960R9S5SK (caffeic acid phenethyl ester); ML9LGA7468 (Phenylethyl Alcohol)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180101
[Lr] Data última revisão:
180101
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171205
[St] Status:MEDLINE


  7 / 1871 MEDLINE  
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[PMID]:28740975
[Au] Autor:Muriana FJG; Montserrat-de la Paz S; Lucas R; Bermudez B; Jaramillo S; Morales JC; Abia R; Lopez S
[Ad] Endereço:Laboratory of Cellular and Molecular Nutrition, Instituto de la Grasa (CSIC), Seville, Spain. serglom@ig.csic.es muriana@ig.csic.es.
[Ti] Título:Tyrosol and its metabolites as antioxidative and anti-inflammatory molecules in human endothelial cells.
[So] Source:Food Funct;8(8):2905-2914, 2017 Aug 01.
[Is] ISSN:2042-650X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Tyrosol (Tyr) is a phenolic compound found in virgin olive oil. After ingestion, Tyr undergoes extensive first pass intestinal/hepatic metabolism. However, knowledge about the biological effects of Tyr metabolites is scarce. We chemically synthesized Tyr glucuronate (Tyr-GLU) and sulphate (Tyr-SUL) metabolites and explored their properties against oxidative stress and inflammation in TNF-α-treated human umbilical vein endothelial cells (hECs). Tyr and Tyr-SUL prevented the rise of reactive oxygen species, the depletion of glutathione, and the down-regulation of glutathione peroxidase 1, glutamate-cysteine ligase catalytic subunit, and heme oxygenase-1 genes. Tyr-SUL and to a lower extent Tyr and Tyr-GLU prevented the phosphorylation of NF-κB signaling proteins. Tyr-GLU and Tyr-SUL also prevented the over-expression of adhesion molecules at gene, protein, and secretory levels, and the adhesion (Tyr-SUL > Tyr-GLU) of human monocytes to hECs. In vivo, Tyr, and most notably Tyr-SUL in a dose-dependent manner, ameliorated plantar and ear edemas in mice models of acute and chronic inflammation. This study demonstrates the antioxidant and/or anti-inflammatory properties of Tyr metabolites, with Tyr-SUL being the most effective.
[Mh] Termos MeSH primário: Anti-Inflamatórios/farmacologia
Antioxidantes/farmacologia
Células Endoteliais/efeitos dos fármacos
Álcool Feniletílico/análogos & derivados
[Mh] Termos MeSH secundário: Anti-Inflamatórios/metabolismo
Antioxidantes/metabolismo
Células Endoteliais/imunologia
Glutamato-Cisteína Ligase/genética
Glutamato-Cisteína Ligase/imunologia
Heme Oxigenase-1/genética
Heme Oxigenase-1/imunologia
Seres Humanos
Estresse Oxidativo/efeitos dos fármacos
Álcool Feniletílico/metabolismo
Álcool Feniletílico/farmacologia
Espécies Reativas de Oxigênio/imunologia
Fator de Necrose Tumoral alfa/genética
Fator de Necrose Tumoral alfa/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Inflammatory Agents); 0 (Antioxidants); 0 (Reactive Oxygen Species); 0 (Tumor Necrosis Factor-alpha); 1AK4MU3SNX (4-hydroxyphenylethanol); EC 1.14.14.18 (Heme Oxygenase-1); EC 6.3.2.2 (Glutamate-Cysteine Ligase); ML9LGA7468 (Phenylethyl Alcohol)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171215
[Lr] Data última revisão:
171215
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170726
[St] Status:MEDLINE
[do] DOI:10.1039/c7fo00641a


  8 / 1871 MEDLINE  
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[PMID]:27770224
[Au] Autor:Wang Z; Bai X; Guo X; He X
[Ad] Endereço:CAS Key Laboratory of Microbial Physiological and Metabolic Engineering, Institute of Microbiology, Chinese Academy of Sciences, Beijing, People's Republic of China.
[Ti] Título:Regulation of crucial enzymes and transcription factors on 2-phenylethanol biosynthesis via Ehrlich pathway in Saccharomyces cerevisiae.
[So] Source:J Ind Microbiol Biotechnol;44(1):129-139, 2017 Jan.
[Is] ISSN:1476-5535
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:2-Phenylethanol (2-PE) is widely used in food, perfume and pharmaceutical industry, but lower production in microbes and less known regulatory mechanisms of 2-PE make further study necessary. In this study, crucial genes like ARO8 and ARO10 of Ehrlich pathway for 2-PE synthesis and key transcription factor ARO80 in Saccharomyces cerevisiae were re-regulated using constitutive promoter; in the meantime, the effect of nitrogen source in synthetic complete (SC) medium with L-phenylalanine (L-Phe) on Aro8/Aro9 and Aro10 was investigated. The results showed that aromatic aminotransferase activities of ARO8 over-expressing strains were seriously inhibited by ammonia sulfate in SC + Phe medium. Flask fermentation test demonstrated that over-expressing ARO8 or ARO10 led to about 42 % increase in 2-PE production when compared with the control strain. Furthermore, influence of transcription factors Cat8 and Mig1 on 2-PE biosynthesis was explored. CAT8 over-expression or MIG1 deletion increased in the transcription of ARO9 and ARO10. 2-PE production of CAT8 over-expressing strain was 62 % higher than that of control strain. Deletion of MIG1 also led to 2-PE biosynthesis enhancement. The strain of CAT8 over-expression and MIG1 deletion was most effective in regulating expression of ARO9 and ARO10. Analysis of mRNA levels and enzyme activities indicates that transaminase in Ehrlich pathway is the crucial target of Nitrogen Catabolize Repression (NCR). Among the engineering strains, the higher 3.73 g/L 2-PE production in CAT8 over-expressing strain without in situ product recovery suggests that the robust strain has potentiality for commercial exploitation.
[Mh] Termos MeSH primário: Álcool Feniletílico/metabolismo
Proteínas de Saccharomyces cerevisiae/metabolismo
Saccharomyces cerevisiae/metabolismo
Fatores de Transcrição/metabolismo
[Mh] Termos MeSH secundário: Carboxiliases/metabolismo
Fermentação
Metabolismo
Fenilalanina/metabolismo
Engenharia de Proteínas/métodos
RNA Mensageiro/metabolismo
Transaminases/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Messenger); 0 (Saccharomyces cerevisiae Proteins); 0 (Transcription Factors); 47E5O17Y3R (Phenylalanine); EC 2.6.1.- (Transaminases); EC 4.1.1.- (Carboxy-Lyases); ML9LGA7468 (Phenylethyl Alcohol)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171211
[Lr] Data última revisão:
171211
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161023
[St] Status:MEDLINE
[do] DOI:10.1007/s10295-016-1852-5


  9 / 1871 MEDLINE  
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[PMID]:28681934
[Au] Autor:Tomiyama R; Takakura K; Takatou S; Le TM; Nishiuchi T; Nakamura Y; Konishi T; Matsugo S; Hori O
[Ad] Endereço:Division of Natural System, Graduate School of Natural Science and Technology, Kanazawa University, Kanazawa, Ishikawa, Japan.
[Ti] Título:3,4-dihydroxybenzalacetone and caffeic acid phenethyl ester induce preconditioning ER stress and autophagy in SH-SY5Y cells.
[So] Source:J Cell Physiol;233(2):1671-1684, 2018 Feb.
[Is] ISSN:1097-4652
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:3,4-dihydroxybenzalacetone (DBL) and Caffeic acid phenethyl ester (CAPE) are both catechol-containing phenylpropanoid derivatives with diverse bioactivities. In the present study, we analyzed the ability of these compounds to activate the unfolded protein response (UPR) and the oxidative stress response. When human SH-SY5Y neuroblastoma cells were treated with DBL or CAPE, the expression of endoplasmic reticulum (ER) stress-related genes such as HSPA5, HYOU1, DDIT3, and SEC61b increased to a larger extent in response to CAPE treatment, while that of antioxidant genes such as HMOX1, GCLM, and NQO1 increased to a larger extent in response to DBL treatment. DNA microarray analysis confirmed the strong link of these compounds to ER stress. Regarding the mechanism, activation of the UPR by these compounds was associated with enhanced levels of oxidized proteins in the ER, and N-acetyl cysteine (NAC), which provides anti-oxidative effects, suppressed the induction of the UPR-target genes. Furthermore, both compounds enhanced the expression of LC3-II, a marker of autophagy, and 4-Phenylbutyric acid (4-PBA), a chemical chaperone that reduces ER stress, suppressed it. Finally, pretreatment of cells with DBL, CAPE or low doses of ER stressors protected cells against a neurotoxin 6-hydroxydopamine (6-OHDA) in an autophagy-dependent manner. These results suggest that DBL and CAPE induce oxidized protein-mediated ER stress and autophagy that may have a preconditioning effect in SH-SY5Y cells.
[Mh] Termos MeSH primário: Autofagia/efeitos dos fármacos
Ácidos Cafeicos/farmacologia
Estresse do Retículo Endoplasmático/efeitos dos fármacos
Neurônios/efeitos dos fármacos
Fármacos Neuroprotetores/farmacologia
Álcool Feniletílico/análogos & derivados
[Mh] Termos MeSH secundário: Linhagem Celular Tumoral
Relação Dose-Resposta a Droga
Regulação da Expressão Gênica/efeitos dos fármacos
Seres Humanos
Proteínas Associadas aos Microtúbulos/genética
Proteínas Associadas aos Microtúbulos/metabolismo
Neurônios/metabolismo
Neurônios/patologia
Estresse Oxidativo/efeitos dos fármacos
Oxidopamina/toxicidade
Álcool Feniletílico/farmacologia
Transdução de Sinais/efeitos dos fármacos
Fatores de Tempo
Resposta a Proteínas não Dobradas/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (3,4-dihydroxybenzalacetone); 0 (Caffeic Acids); 0 (MAP1LC3A protein, human); 0 (Microtubule-Associated Proteins); 0 (Neuroprotective Agents); 8HW4YBZ748 (Oxidopamine); G960R9S5SK (caffeic acid phenethyl ester); ML9LGA7468 (Phenylethyl Alcohol)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171128
[Lr] Data última revisão:
171128
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170707
[St] Status:MEDLINE
[do] DOI:10.1002/jcp.26080


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[PMID]:29016623
[Au] Autor:Schriever VA; Han P; Weise S; Hösel F; Pellegrino R; Hummel T
[Ad] Endereço:Smell & Taste Clinic, Department of Otorhinolaryngology, Technical University of Dresden, Dresden, Germany.
[Ti] Título:Time frequency analysis of olfactory induced EEG-power change.
[So] Source:PLoS One;12(10):e0185596, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:OBJECTIVES: The objective of the present study was to investigate the usefulness of time-frequency analysis (TFA) of olfactory-induced EEG change with a low-cost, portable olfactometer in the clinical investigation of smell function. MATERIALS & METHODS: A total of 78 volunteers participated. The study was composed of three parts where olfactory stimuli were presented using a custom-built olfactometer. Part I was designed to optimize the stimulus as well as the recording conditions. In part II EEG-power changes after olfactory/trigeminal stimulation were compared between healthy participants and patients with olfactory impairment. In Part III the test-retest reliability of the method was evaluated in healthy subjects. RESULTS: Part I indicated that the most effective paradigm for stimulus presentation was cued stimulus, with an interstimulus interval of 18-20s at a stimulus duration of 1000ms with each stimulus quality presented 60 times in blocks of 20 stimuli each. In Part II we found that central processing of olfactory stimuli analyzed by TFA differed significantly between healthy controls and patients even when controlling for age. It was possible to reliably distinguish patients with olfactory impairment from healthy individuals at a high degree of accuracy (healthy controls vs anosmic patients: sensitivity 75%; specificity 89%). In addition we could show a good test-retest reliability of TFA of chemosensory induced EEG-power changes in Part III. CONCLUSIONS: Central processing of olfactory stimuli analyzed by TFA reliably distinguishes patients with olfactory impairment from healthy individuals at a high degree of accuracy. Importantly this can be achieved with a simple olfactometer.
[Mh] Termos MeSH primário: Eletroencefalografia/métodos
Transtornos do Olfato/diagnóstico
Olfatometria/métodos
Percepção Olfatória/fisiologia
[Mh] Termos MeSH secundário: Adolescente
Adulto
Idoso
Estudos de Casos e Controles
Sinais (Psicologia)
Cicloexanóis/análise
Eletroencefalografia/instrumentação
Feminino
Voluntários Saudáveis
Seres Humanos
Masculino
Meia-Idade
Monoterpenos/análise
Transtornos do Olfato/fisiopatologia
Olfatometria/instrumentação
Álcool Feniletílico/análise
Sensibilidade e Especificidade
Olfato/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cyclohexanols); 0 (Monoterpenes); ML9LGA7468 (Phenylethyl Alcohol); RV6J6604TK (eucalyptol)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
171031
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171011
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0185596



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