[PMID]: | 27388659 |
[Au] Autor: | Miranda LF; Domingues DS; Queiroz ME |
[Ad] Endereço: | Departamento de Química, Faculdade de Filosofia Ciência e Letras de Ribeirão Preto, Universidade de São Paulo, P.O. Box 14040-901, Ribeirão Preto, SP, Brazil. |
[Ti] Título: | Selective solid-phase extraction using molecularly imprinted polymers for analysis of venlafaxine, O-desmethylvenlafaxine, and N-desmethylvenlafaxine in plasma samples by liquid chromatography-tandem mass spectrometry. |
[So] Source: | J Chromatogr A;1458:46-53, 2016 Aug 05. |
[Is] ISSN: | 1873-3778 |
[Cp] País de publicação: | Netherlands |
[La] Idioma: | eng |
[Ab] Resumo: | This paper focuses on the development of a novel miniaturized molecularly imprinted solid-phase extraction (MISPE) and ultra high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method to determine venlafaxine (VEN), O-desmethylvenlafaxine (ODV), and N-desmethylvenlafaxine (NDV) in plasma samples. The molecularly imprinted polymer (MIP) was prepared by the precipitation polymerization approach; VEN, metacrylic acid, ethylene glycol dimethacrylate, 2,2-azobisisobutyronitrile, and toluene were used as template, monomer, crosslinker, initiator, and porogen solvent, respectively. MIP and of the non-imprinted control polymer (NIP) sorbents were characterized by Fourier transform infrared spectroscopy and scanning electron microscopy. MIP phase presented higher extraction efficiency (MISPE, using plasma samples spiked with VEN) than the NIP phase (84 and 49% recovery rates, respectively). Analysis of other antidepressants with different chemical structures by MISPE-UHPLC-MS/MS attested to the selectivity of the developed MIP. The developed method presented precision assays with coefficients of variation (CV) smaller than 15%; accuracy assays with relative standard error (RSE%) values ranging from -12 to 16%, and linear ranges from 3 to 700ngmL(-1) for VEN, from 5 to 700ngmL(-1) for ODV, and from 3 to 500ngmL(-1) for NDV. The coefficients of determination (r(2)) were higher than 0.995. The lack-of-fit test also attested to the linearity of this method. This method was successfully applied to determine VEN, NDV, and ODV in plasma samples from depressed patients undergoing therapy with VEN. |
[Mh] Termos MeSH primário: |
Cicloexanóis/sangue Succinato de Desvenlafaxina/sangue Impressão Molecular Polímeros/química Extração em Fase Sólida/métodos Espectrometria de Massas em Tandem/métodos Cloridrato de Venlafaxina/sangue
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[Mh] Termos MeSH secundário: |
Acrilatos/química Antidepressivos/sangue Antidepressivos/química Antidepressivos/uso terapêutico Cromatografia Líquida de Alta Pressão Cicloexanóis/metabolismo Depressão/sangue Depressão/tratamento farmacológico Succinato de Desvenlafaxina/metabolismo Seres Humanos Metacrilatos/química Microscopia Eletrônica de Varredura Nitrilos/química Polimerização Espectroscopia de Infravermelho com Transformada de Fourier Tolueno/química Cloridrato de Venlafaxina/metabolismo Cloridrato de Venlafaxina/farmacocinética Cloridrato de Venlafaxina/uso terapêutico
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[Pt] Tipo de publicação: | JOURNAL ARTICLE |
[Nm] Nome de substância:
| 0 (Acrylates); 0 (Antidepressive Agents); 0 (Cyclohexanols); 0 (Methacrylates); 0 (N-desmethylvenlafaxine); 0 (Nitriles); 0 (Polymers); 3FPU23BG52 (Toluene); 7BK5G69305 (ethylene dimethacrylate); 7D7RX5A8MO (Venlafaxine Hydrochloride); FZ6PX8U5YB (azobis(isobutyronitrile)); J94PBK7X8S (acrylic acid); ZB22ENF0XR (Desvenlafaxine Succinate) |
[Em] Mês de entrada: | 1612 |
[Cu] Atualização por classe: | 161230 |
[Lr] Data última revisão:
| 161230 |
[Sb] Subgrupo de revista: | IM |
[Da] Data de entrada para processamento: | 160709 |
[St] Status: | MEDLINE |
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