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[PMID]:29374173
[Au] Autor:Shin JH; Kim D; Jung MW
[Ad] Endereço:Graduate School of Medical Science and Engineering, Korea Advanced Institute of Science and Technology, Daejeon, 34141, Korea.
[Ti] Título:Differential coding of reward and movement information in the dorsomedial striatal direct and indirect pathways.
[So] Source:Nat Commun;9(1):404, 2018 01 26.
[Is] ISSN:2041-1723
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The direct and indirect pathways of the basal ganglia have long been thought to mediate behavioral promotion and inhibition, respectively. However, this classic dichotomous model has been recently challenged. To better understand neural processes underlying reward-based learning and movement control, we recorded from direct (dSPNs) and indirect (iSPNs) pathway spiny projection neurons in the dorsomedial striatum of D1-Cre and D2-Cre mice performing a probabilistic Pavlovian conditioning task. dSPNs tend to increase activity while iSPNs decrease activity as a function of reward value, suggesting the striatum represents value in the relative activity levels of dSPNs versus iSPNs. Lick offset-related activity increase is largely dSPN selective, suggesting dSPN involvement in suppressing ongoing licking behavior. Rapid responses to negative outcome and previous reward-related responses are more frequent among iSPNs than dSPNs, suggesting stronger contributions of iSPNs to outcome-dependent behavioral adjustment. These findings provide new insights into striatal neural circuit operations.
[Mh] Termos MeSH primário: Condicionamento (Psicologia)/fisiologia
Corpo Estriado/fisiologia
Vias Neurais/fisiologia
Neurônios/fisiologia
[Mh] Termos MeSH secundário: Animais
Corpo Estriado/citologia
Sinais (Psicologia)
Eletrodos Implantados
Masculino
Camundongos
Camundongos Endogâmicos C57BL
Camundongos Transgênicos
Monoterpenos
Movimento
Odorantes/análise
Percepção Olfatória/fisiologia
Optogenética
Pentanóis
Receptores de Dopamina D1/fisiologia
Receptores de Dopamina D2/fisiologia
Recompensa
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Monoterpenes); 0 (Pentanols); 0 (Receptors, Dopamine D1); 0 (Receptors, Dopamine D2); 75GK9XIA8I (carvone); T7EU0O9VPP (citral); Z135787824 (isoamyl acetate)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180227
[Lr] Data última revisão:
180227
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180128
[St] Status:MEDLINE
[do] DOI:10.1038/s41467-017-02817-1


  2 / 1067 MEDLINE  
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[PMID]:28407625
[Au] Autor:Wigger-Alberti W; Williams R; von Mackensen YL; Hoffman-Wecker M; Grossmann U; Staedtler G; Nkulikiyinka R; Shakery K
[Ad] Endereço:bioskin GmbH, Hamburg, Germany.
[Ti] Título:Comparison of Occlusive and Open Application in a Psoriasis Plaque Test Design, Exemplarily Using Investigations of Mapracorat 0.1% Ointment versus Vehicle and Reference Drugs.
[So] Source:Skin Pharmacol Physiol;30(2):102-114, 2017.
[Is] ISSN:1660-5535
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:BACKGROUND/AIMS: Psoriasis plaque tests (PPTs) are important tools in the early phases of antipsoriatic drug development. Two distinct PPT design variants (open vs. occluded drug application) are commonly used, but no previous work has aimed to directly compare and contrast their performance. METHODS: We compared the antipsoriatic efficacy of mapracorat 0.1% ointment and reference drugs reported in 2 separate studies, representing open and occluded PPT designs. The drug effect size was measured by sonography (mean change in echo-poor band thickness), chromametry, and standardized clinical assessment. RESULTS: Antipsoriatic effects were detectable for the study drugs in both occluded and open PPTs. Differences between the potency of antipsoriatic drugs and vehicle were observable. The total antipsoriatic effect size appeared to be higher in the occluded PPT than the open PPT, despite the shorter treatment duration (2 vs. 4 weeks). Effect dynamics over time revealed greater differences between some study drugs in the open PPT compared to the occluded PPT. CONCLUSION: Taking the higher technical challenges for the open PPT into account, we recommend the occluded PPT as a standard screening setting in early drug development. In special cases, considering certain drug aspects or study objectives that would require procedural adaptations, an open PPT could be the better-suited design. Finally, both PPT models show clear advantages: classification as phase I studies, small number of psoriatic subjects, relatively short study duration, excellent discrimination between compounds and concentrations, parallel measurement of treatment response, and go/no go decisions very early in clinical development.
[Mh] Termos MeSH primário: Benzofuranos/farmacologia
Fármacos Dermatológicos/farmacologia
Modelos Biológicos
Pentanóis/farmacologia
Psoríase/tratamento farmacológico
Quinolinas/farmacologia
[Mh] Termos MeSH secundário: Adulto
Idoso
Benzofuranos/administração & dosagem
Fármacos Dermatológicos/administração & dosagem
Método Duplo-Cego
Desenho de Drogas
Feminino
Seres Humanos
Masculino
Meia-Idade
Pomadas
Pentanóis/administração & dosagem
Psoríase/patologia
Quinolinas/administração & dosagem
Projetos de Pesquisa
Resultado do Tratamento
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE; RANDOMIZED CONTROLLED TRIAL
[Nm] Nome de substância:
0 (Benzofurans); 0 (Dermatologic Agents); 0 (Ointments); 0 (Pentanols); 0 (Quinolines); 0 (R-1,1,1-trifluoro-4-(5-fluoro-2,3-dihydrobenzofuran-7-yl)-4-methyl-2-(((2-methyl-5-quinolyl)amino)methyl)pentan-2-ol)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171030
[Lr] Data última revisão:
171030
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170414
[St] Status:MEDLINE
[do] DOI:10.1159/000458415


  3 / 1067 MEDLINE  
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[PMID]:28386884
[Au] Autor:Devarajan Y; Munuswamy DB; Nagappan B
[Ad] Endereço:Department of Mechanical Engineering, Vel Tech Dr.RR & Dr.SR University, Chennai, India. dyuvarajan2@gmail.com.
[Ti] Título:Emissions analysis on diesel engine fuelled with cashew nut shell biodiesel and pentanol blends.
[So] Source:Environ Sci Pollut Res Int;24(14):13136-13141, 2017 May.
[Is] ISSN:1614-7499
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:The present work is intended to investigate the emission characteristics of neat cashew nut shell methyl ester (CNSME100) by adding pentanol at two different proportions and compared with the baseline diesel. CNSME100 is prepared by the conventional transesterification process. CNSME100 is chosen due to its non-edible nature. Pentanol is chosen as an additive because of its higher inbuilt oxygen content and surface to volume ratio which reduces the drawbacks of neat CNSME100. Emission characteristics were carried out in single cylinder naturally aspirated CI engine fuelled with neat cashew nut shell methyl ester (CNSME), cashew nut shell methyl ester and pentanol by 10% volume (CNSME90P10), cashew nut shell methyl ester and pentanol by 20% volume (CNSME80P20), and diesel. This work also aims to investigate the feasibility of operating an engine fuelled with neat methyl ester and alcohol blends. Experimental results showed that by blending higher alcohol to neat cashew nut shell methyl ester reduces the emissions significantly. It is also found that the emission from neat methyl ester and pentanol blends is lesser than diesel at all loads.
[Mh] Termos MeSH primário: Biocombustíveis
Emissões de Veículos
[Mh] Termos MeSH secundário: Anacardium
Gasolina
Nozes
Pentanóis
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biofuels); 0 (Gasoline); 0 (Pentanols); 0 (Vehicle Emissions)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170605
[Lr] Data última revisão:
170605
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170408
[St] Status:MEDLINE
[do] DOI:10.1007/s11356-017-8915-7


  4 / 1067 MEDLINE  
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[PMID]:28366786
[Au] Autor:Di C; Ning C; Huang LQ; Wang CZ
[Ad] Endereço:State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, PR China; College of Life Sciences, University of Chinese Academy of Sciences, Beijing, PR China.
[Ti] Título:Design of larval chemical attractants based on odorant response spectra of odorant receptors in the cotton bollworm.
[So] Source:Insect Biochem Mol Biol;84:48-62, 2017 May.
[Is] ISSN:1879-0240
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Lepidopteran caterpillars rely on olfaction and gustation to discriminate among food sources. Compared to the larval gustation, the larval olfaction has been poorly investigated. To uncover the molecular basis of olfaction in Helicoverpa armigera larvae, we identified 17 odorant receptor (Or) genes in larval antennae and maxillae using transcriptome sequencing, and functionally identified the response spectra of seven Ors to ecologically relevant odorants. Innate behavioural responses of larvae to active odorants were evaluated in chemotaxis assays. Several odorant blends were formulated based on the Ors tuning spectra and caterpillar chemotaxis. A four-component blend strongly attracted H. armigera larvae, and cis-jasmone and 1-pentanol were identified as essential components. Localization analyses showed that the two Ors detecting these components (Or41 and Or52) were expressed in the same sensory neurons. This is the first evidence that Ors in a polyphagous caterpillar respond to odorants in a combinatorial manner. The design of attractants to target specific olfactory pathways may promote the development of new baits for pest management.
[Mh] Termos MeSH primário: Antenas de Artrópodes/metabolismo
Mariposas/metabolismo
Receptores Odorantes/metabolismo
Olfato
[Mh] Termos MeSH secundário: Animais
Ciclopentanos
Feminino
Larva/genética
Larva/metabolismo
Masculino
Mariposas/genética
Oxilipinas
Pentanóis
Receptores Odorantes/genética
Xenopus
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cyclopentanes); 0 (Oxylipins); 0 (Pentanols); 0 (Receptors, Odorant); M9L931X26Y (n-pentanol); RC4W0G9YUK (jasmone)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170404
[St] Status:MEDLINE


  5 / 1067 MEDLINE  
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[PMID]:28364792
[Au] Autor:Karami N; Karimi A; Aliahmadi A; Mirzajan F; Rezadoost H; Ghassempour A; Fallah F
[Ad] Endereço:Pediatric Infections Research Center, Mofid Children Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
[Ti] Título:Identification of bacteria using volatile organic compounds.
[So] Source:Cell Mol Biol (Noisy-le-grand);63(2):112-121, 2017 Feb 28.
[Is] ISSN:1165-158X
[Cp] País de publicação:France
[La] Idioma:eng
[Ab] Resumo:The rapid diagnosis of respiratory infections has always been an important goal for medical professionals, because rapid and accurate diagnosis leads to proper and timely treatment, and consequently, reduces the costs of incorrect and long-term treatments, and antibiotic resistance. The present study was conducted with the aim of detecting volatile organic compounds (VOCs) in three bacteria: Pseudomonas aeruginosa, Acinetobacter baumannii, Klebsiella pneumoniae. Headspace of the studied bacteria, after separately culturing in two types of liquid medium in three different time-periods, was extracted by solid phase microextraction and analysed by gas chromatography mass spectrometry The analysis results of the VOCs produced by the studied bacteria indicate that some VOCs are common and some are unique in each bacterium. 1-penten-3-ol, levomenthol, and 2-octyl-1-ol for P. aeruginosa, cyclohexene, 4-ethenyl, and cis-Dihydro-α-terpinyl acetate for A. baumannii and 1,3-butadiene, butyraldehyde, longifolene, octyl acetate, tridecanol, dodecenal, (E)-2-hexyl ester, butanoic acid, and 5,5-dodecadinyl-1 12-diol for K. pneumoniae were identified as unique VOCs for each bacterium. Finally, it can be said that an accurate and rapid bacterial detection method can be achieved by using a tool that can detect bacterial VOCs. However, more studies are needed to design a tool for which all aspects have been assessed, so that it can give us a more complete pattern for the use of these compounds as biomarkers.
[Mh] Termos MeSH primário: Acinetobacter baumannii/química
Klebsiella pneumoniae/química
Pseudomonas aeruginosa/química
Compostos Orgânicos Voláteis/análise
[Mh] Termos MeSH secundário: Acetatos/análise
Acetatos/isolamento & purificação
Aldeídos/análise
Aldeídos/isolamento & purificação
Infecções Bacterianas/microbiologia
Butadienos/análise
Butadienos/isolamento & purificação
Ácido Butírico/análise
Ácido Butírico/isolamento & purificação
Cicloexenos/análise
Cicloexenos/isolamento & purificação
Cromatografia Gasosa-Espectrometria de Massas
Seres Humanos
Pentanóis/análise
Pentanóis/isolamento & purificação
Sistema Respiratório/microbiologia
Sesquiterpenos/análise
Sesquiterpenos/isolamento & purificação
Microextração em Fase Sólida
Especificidade da Espécie
Compostos Orgânicos Voláteis/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Acetates); 0 (Aldehydes); 0 (Butadienes); 0 (Cyclohexenes); 0 (Pentanols); 0 (Sesquiterpenes); 0 (Volatile Organic Compounds); 107-92-6 (Butyric Acid); 12L0P8F7GN (cyclohexene); 3YXH7YY8WM (longifolene); DYU6Q1758M (1-penten-3-ol); H21352682A (butyraldehyde); JSD5FGP5VD (1,3-butadiene); X0FN2J413S (octyl acetate)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170426
[Lr] Data última revisão:
170426
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170403
[St] Status:MEDLINE
[do] DOI:10.14715/cmb/2017.63.2.18


  6 / 1067 MEDLINE  
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[PMID]:28323874
[Au] Autor:Urlacher E; Devaud JM; Mercer AR
[Ad] Endereço:University of Otago, Department of Zoology, Dunedin, New Zealand.
[Ti] Título:C-type allatostatins mimic stress-related effects of alarm pheromone on honey bee learning and memory recall.
[So] Source:PLoS One;12(3):e0174321, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:As honey bee populations worldwide are declining there is an urgent need for a deeper understanding of stress reactivity in these important insects. Our data indicate that stress responses in bees (Apis mellifera L.) may be mediated by neuropeptides identified, on the basis of sequence similarities, as allatostatins (ASTA, ASTC and ASTCC). Effects of allatostatin injection are compared with stress-related changes in learning performance induced by the honeybee alarm pheromone, isopentylacetate (IPA). We find that bees can exhibit two markedly different responses to IPA, with opposing effects on learning behaviour and memory generalisation, and that strikingly similar responses can be elicited by allatostatins, in particular ASTCC. These findings lend support to the hypothesis that allatostatins mediate stress reactivity in honey bees and suggest responses to stress in these insects are state dependent.
[Mh] Termos MeSH primário: Abelhas
Memória/fisiologia
Rememoração Mental
Neuropeptídeos/metabolismo
Pentanóis/metabolismo
Feromônios/metabolismo
Estresse Fisiológico/fisiologia
[Mh] Termos MeSH secundário: Animais
Comportamento Animal/fisiologia
Aprendizagem/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Neuropeptides); 0 (Pentanols); 0 (Pheromones); 110119-33-0 (allatostatin); Z135787824 (isoamyl acetate)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170926
[Lr] Data última revisão:
170926
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170322
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0174321


  7 / 1067 MEDLINE  
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[PMID]:28254164
[Au] Autor:Andersson L; Claeson AS; Nyberg L; Nordin S
[Ad] Endereço:Department of Psychology, Umeå University, Umeå, Sweden; Department of Occupational and Public Health Sciences, University of Gävle, Sweden. Electronic address: linus.andersson@umu.se.
[Ti] Título:Short-term olfactory sensitization involves brain networks relevant for pain, and indicates chemical intolerance.
[So] Source:Int J Hyg Environ Health;220(2 Pt B):503-509, 2017 Apr.
[Is] ISSN:1618-131X
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Chemical intolerance is a medically unexplained affliction that implies deleterious reactions to non-toxic everyday chemical exposure. Sensitization (i.e. increased reactivity to repeated, invariant stimulation) to odorous stimulation is an important component in theoretical explanations of chemical intolerance, but empirical evidence is scarce. We hypothesized that (1) individuals who sensitize to repeated olfactory stimulation, compared with those who habituate, would express a lower blood oxygenated level dependent (BOLD) response in key inhibitory areas such as the rACC, and higher signal in pain/saliency detection regions, as well as primary and/or secondary olfactory projection areas; and (2) olfactory sensitization, compared with habituation, would be associated with greater self-reported chemical intolerance. Moreover, we assessed whether olfactory sensitization was paralleled by comparable trigeminal processing - in terms of perceptual ratings and BOLD responses. We grouped women from a previous functional magnetic imaging study based on intensity ratings of repeated amyl acetate exposure over time. Fourteen women sensitized to the exposure, 15 habituated, and 20 were considered "intermediate" (i.e. neither sensitizers nor habituaters). Olfactory sensitizers, compared with habituaters, displayed a BOLD-pattern in line with the hypothesis, and reported greater problems with odours in everyday life. They also expressed greater reactions to CO in terms of both perceived intensity and BOLD signal. The similarities with pain are discussed.
[Mh] Termos MeSH primário: Encéfalo/fisiologia
Odorantes
Dor/fisiopatologia
[Mh] Termos MeSH secundário: Adulto
Idoso
Encéfalo/diagnóstico por imagem
Dióxido de Carbono/farmacologia
Feminino
Seres Humanos
Imagem por Ressonância Magnética
Meia-Idade
Dor/diagnóstico por imagem
Pentanóis/farmacologia
Olfato
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Pentanols); 142M471B3J (Carbon Dioxide); 92Q24NH7AS (amyl acetate)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170911
[Lr] Data última revisão:
170911
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170304
[St] Status:MEDLINE


  8 / 1067 MEDLINE  
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[PMID]:28209513
[Au] Autor:Kurino C; Furuhashi T; Sudoh K; Sakamoto K
[Ad] Endereço:Graduate School of Life and Environmental Sciences, University of Tsukuba, Japan.
[Ti] Título:Isoamyl alcohol odor promotes longevity and stress tolerance via DAF-16 in Caenorhabditis elegans.
[So] Source:Biochem Biophys Res Commun;485(2):395-399, 2017 Apr 01.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The possibility that odor plays a role in lifespan regulation through effects on the nervous system is indicated by research on Caenorhabditis elegans. In fact, ablation of AWA and AWC, which are suggested as olfactory neurons, has been shown to extend lifespan via DAF-16, a homolog of FoxO. However, the effects of odor stimuli on the lifespan still remain unclear. Thus, we here aimed to clarify the effect of attractive and repulsive odors on longevity and stress tolerance in C. elegans and to analyze the pathways thereof. We used isoamyl alcohol as an attractive odor, and acetic acid as a repellent component, as identified by chemotaxis assay. We found that isoamyl alcohol stimulus promoted longevity in a DAF-16-dependent manner. On the other hand, acetic acid stimulus promoted thermotolerance through mechanisms independent of DAF-16. Above all, our results indicate that odor stimuli affect the lifespan and stress tolerance of C. elegans, with attractive and repulsive odors exerting their effects through different mechanisms, and that longevity is induced by both activation and inactivation of olfactory neurons.
[Mh] Termos MeSH primário: Proteínas de Caenorhabditis elegans/metabolismo
Caenorhabditis elegans/efeitos dos fármacos
Fatores de Transcrição Forkhead/metabolismo
Longevidade/efeitos dos fármacos
Odorantes
Pentanóis/farmacologia
[Mh] Termos MeSH secundário: Ácido Acético/farmacologia
Adaptação Fisiológica/efeitos dos fármacos
Adaptação Fisiológica/fisiologia
Animais
Caenorhabditis elegans/genética
Caenorhabditis elegans/fisiologia
Proteínas de Caenorhabditis elegans/genética
Quimiotaxia/efeitos dos fármacos
Relação Dose-Resposta a Droga
Fatores de Transcrição Forkhead/genética
Expressão Gênica/efeitos dos fármacos
Proteínas de Choque Térmico/genética
Proteínas de Choque Térmico/metabolismo
Temperatura Alta
Mutação
Neurônios Receptores Olfatórios/efeitos dos fármacos
Neurônios Receptores Olfatórios/metabolismo
Neurônios Receptores Olfatórios/fisiologia
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Transdução de Sinais/efeitos dos fármacos
Estresse Fisiológico/fisiologia
Superóxido Dismutase/genética
Superóxido Dismutase/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Caenorhabditis elegans Proteins); 0 (Forkhead Transcription Factors); 0 (Heat-Shock Proteins); 0 (Pentanols); 0 (daf-16 protein, C elegans); 0 (hsp-12.6 protein, C elegans); DEM9NIT1J4 (isopentyl alcohol); EC 1.15.1.1 (Sod-3 protein, C elegans); EC 1.15.1.1 (Superoxide Dismutase); Q40Q9N063P (Acetic Acid)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170613
[Lr] Data última revisão:
170613
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170218
[St] Status:MEDLINE


  9 / 1067 MEDLINE  
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[PMID]:28168624
[Au] Autor:Malhadas C; Malheiro R; Pereira JA; de Pinho PG; Baptista P
[Ad] Endereço:REQUIMTE-LAQV, School of Agriculture, Polytechnic Institute of Bragança, Campus de Santa Apolónia, 5300-253, Bragança, Portugal.
[Ti] Título:Antimicrobial activity of endophytic fungi from olive tree leaves.
[So] Source:World J Microbiol Biotechnol;33(3):46, 2017 Mar.
[Is] ISSN:1573-0972
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:In this study, the antimicrobial potential of three fungal endophytes from leaves of Olea europaea L. was evaluated and the host plant extract effect in the antimicrobial activity was examined. The volatile compounds produced by endophytes were identified by GC/MS and further correlated with the antimicrobial activity. In potato dextrose agar, both Penicillium commune and Penicillium canescens were the most effective inhibiting Gram-positive and -negative bacteria (up to 2.7-fold compared to 30 µg/mL chloramphenicol), whereas Alternaria alternata was most effective inhibiting yeasts (up to 8.0-fold compared to 25 µg/mL fluconazole). The presence of aqueous leaf extract in culture medium showed to induce or repress the antimicrobial activity, depending on the endophytic species. In the next step, various organic extracts from both A. alternata mycelium and cultured broth were prepared; being ethyl acetate extracts displayed the widest spectrum of anti-microorganisms at a minimum inhibitory concentration ≤0.095 mg/mL. The volatile composition of the fungi that displayed the highest (A. alternata) and the lowest (P. canescens) antimicrobial activity against yeasts revealed the presence of six volatiles, being the most abundant components (3-methyl-1-butanol and phenylethyl alcohol) ascribed with antimicrobial potentialities. Overall the results highlighted for the first time the antimicrobial potential of endophytic fungi from O. europaea and the possibility to be exploited for their antimicrobial agents.
[Mh] Termos MeSH primário: Endófitos/metabolismo
Fungos/fisiologia
Bactérias Gram-Negativas/efeitos dos fármacos
Bactérias Gram-Positivas/efeitos dos fármacos
Olea/microbiologia
Extratos Vegetais/farmacologia
[Mh] Termos MeSH secundário: Acetatos/química
Alternaria/efeitos dos fármacos
Cloranfenicol/farmacologia
Fluconazol/farmacologia
Testes de Sensibilidade Microbiana
Olea/química
Penicillium/metabolismo
Pentanóis/farmacologia
Álcool Feniletílico/farmacologia
Extratos Vegetais/química
Extratos Vegetais/isolamento & purificação
Folhas de Planta/microbiologia
Compostos Orgânicos Voláteis/química
Compostos Orgânicos Voláteis/farmacologia
Leveduras/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Acetates); 0 (Pentanols); 0 (Plant Extracts); 0 (Volatile Organic Compounds); 66974FR9Q1 (Chloramphenicol); 76845O8NMZ (ethyl acetate); 8VZV102JFY (Fluconazole); DEM9NIT1J4 (isopentyl alcohol); ML9LGA7468 (Phenylethyl Alcohol)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:171102
[Lr] Data última revisão:
171102
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170208
[St] Status:MEDLINE
[do] DOI:10.1007/s11274-017-2216-7


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[PMID]:28088865
[Au] Autor:Güray MZ; Zheng S; Doucette AA
[Ad] Endereço:Department of Chemistry, Dalhousie University , PO Box 15000, 6274 Coburg Road, Halifax, Nova Scotia B3H 4R2, Canada.
[Ti] Título:Mass Spectrometry of Intact Proteins Reveals +98 u Chemical Artifacts Following Precipitation in Acetone.
[So] Source:J Proteome Res;16(2):889-897, 2017 Feb 03.
[Is] ISSN:1535-3907
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Protein precipitation in acetone is frequently employed ahead of mass spectrometry for sample preconcentration and purification. Unfortunately, acetone is not chemically inert; mass artifacts have previously been observed on glycine-containing peptides when exposed to acetone under acidic conditions. We herein report a distinct chemical modification occurring at the level of intact proteins when incubated in acetone. This artifact manifests as one or more satellite peaks in the MS spectrum of intact protein, spaced 98 u above the mass of the unmodified protein. Other artifacts (+84, +112 u) also appear upon incubation of proteins or peptides in acetone. The reaction is pH-sensitive, being suppressed when proteins are exposed to acetone under acidic conditions. The +98 u artifact is speculated to originate through an intermediate product of aldol condensation of acetone to form diacetone alcohol and mesityl oxide. A +98 u product could originate from nucleophilic attack on mesityl oxide or through condensation with diacetone alcohol. Given the extent of modification possible upon exposure of proteins to acetone, particularly following overnight solvent exposure or incubation at room temperature, an awareness of the variables influencing this novel modification is valued by proteomics researchers who employ acetone precipitation for protein purification.
[Mh] Termos MeSH primário: Acetona/química
Artefatos
Citocromos c/análise
Proteínas de Escherichia coli/análise
Espectrometria de Massas/normas
Peptídeos/análise
[Mh] Termos MeSH secundário: Animais
Precipitação Química
Citocromos c/química
Escherichia coli/química
Proteínas de Escherichia coli/química
Hemoglobinas/análise
Hemoglobinas/química
Hexanonas/química
Concentração de Íons de Hidrogênio
Mioglobina/análise
Mioglobina/química
Pentanóis/química
Pentanonas/química
Peptídeos/química
Proteômica/métodos
Espectrometria de Massas por Ionização por Electrospray/normas
Ubiquitina/análise
Ubiquitina/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Escherichia coli Proteins); 0 (Hemoglobins); 0 (Hexanones); 0 (Myoglobin); 0 (Pentanols); 0 (Pentanones); 0 (Peptides); 0 (Ubiquitin); 1364PS73AF (Acetone); 77LAC84669 (mesityl oxide); 9007-43-6 (Cytochromes c); Q7WP157PTD (diacetone alcohol)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171016
[Lr] Data última revisão:
171016
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170117
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jproteome.6b00841



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