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[PMID]:29374183
[Au] Autor:Joo S; Cho IJ; Seo H; Son HF; Sagong HY; Shin TJ; Choi SY; Lee SY; Kim KJ
[Ad] Endereço:School of Life Sciences (KNU Creative BioResearch Group), KNU Institute for Microorganisms, Kyungpook National University, Daehak-ro 80, Buk-gu, Daegu, 41566, Republic of Korea.
[Ti] Título:Structural insight into molecular mechanism of poly(ethylene terephthalate) degradation.
[So] Source:Nat Commun;9(1):382, 2018 01 26.
[Is] ISSN:2041-1723
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Plastics, including poly(ethylene terephthalate) (PET), possess many desirable characteristics and thus are widely used in daily life. However, non-biodegradability, once thought to be an advantage offered by plastics, is causing major environmental problem. Recently, a PET-degrading bacterium, Ideonella sakaiensis, was identified and suggested for possible use in degradation and/or recycling of PET. However, the molecular mechanism of PET degradation is not known. Here we report the crystal structure of I. sakaiensis PETase (IsPETase) at 1.5 Å resolution. IsPETase has a Ser-His-Asp catalytic triad at its active site and contains an optimal substrate binding site to accommodate four monohydroxyethyl terephthalate (MHET) moieties of PET. Based on structural and site-directed mutagenesis experiments, the detailed process of PET degradation into MHET, terephthalic acid, and ethylene glycol is suggested. Moreover, other PETase candidates potentially having high PET-degrading activities are suggested based on phylogenetic tree analysis of 69 PETase-like proteins.
[Mh] Termos MeSH primário: Proteínas de Bactérias/química
Burkholderiales/enzimologia
Poluentes Ambientais/química
Hidrolases/química
Polietilenotereftalatos/química
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Proteínas de Bactérias/genética
Proteínas de Bactérias/metabolismo
Biodegradação Ambiental
Burkholderiales/química
Domínio Catalítico
Clonagem Molecular
Cristalografia por Raios X
Poluentes Ambientais/metabolismo
Escherichia coli/genética
Escherichia coli/metabolismo
Etilenoglicol/química
Etilenoglicol/metabolismo
Expressão Gênica
Hidrolases/genética
Hidrolases/metabolismo
Cinética
Simulação de Acoplamento Molecular
Ácidos Ftálicos/química
Ácidos Ftálicos/metabolismo
Polietilenotereftalatos/metabolismo
Ligação Proteica
Domínios e Motivos de Interação entre Proteínas
Estrutura Secundária de Proteína
Proteínas Recombinantes/química
Proteínas Recombinantes/genética
Proteínas Recombinantes/metabolismo
Alinhamento de Sequência
Homologia de Sequência de Aminoácidos
Especificidade por Substrato
Termodinâmica
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Environmental Pollutants); 0 (Phthalic Acids); 0 (Polyethylene Terephthalates); 0 (Recombinant Proteins); 6S7NKZ40BQ (terephthalic acid); EC 3.- (Hydrolases); FC72KVT52F (Ethylene Glycol)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180222
[Lr] Data última revisão:
180222
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180128
[St] Status:MEDLINE
[do] DOI:10.1038/s41467-018-02881-1


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[PMID]:29304068
[Au] Autor:Drake AC; Lee Y; Burgess EM; Karlsson JOM; Eroglu A; Higgins AZ
[Ad] Endereço:School of Chemical, Biological and Environmental Engineering, Oregon State University, Corvallis, Oregon, United States of America.
[Ti] Título:Effect of water content on the glass transition temperature of mixtures of sugars, polymers, and penetrating cryoprotectants in physiological buffer.
[So] Source:PLoS One;13(1):e0190713, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Long-term storage of viable mammalian cells is important for applications ranging from in vitro fertilization to cell therapy. Cryopreservation is currently the most common approach, but storage in liquid nitrogen is relatively costly and the requirement for low temperatures during shipping is inconvenient. Desiccation is an alternative strategy with the potential to enable viable cell preservation at more convenient storage temperatures without the need for liquid nitrogen. To achieve stability during storage in the dried state it is necessary to remove enough water that the remaining matrix forms a non-crystalline glassy solid. Thus, the glass transition temperature is a key parameter for design of cell desiccation procedures. In this study, we have investigated the effects of moisture content on the glass transition temperature (Tg) of mixtures of sugars (trehalose or raffinose), polymers (polyvinylpyrrolidone or Ficoll), penetrating cryoprotectants (ethylene glycol, propylene glycol, or dimethyl sulfoxide), and phosphate buffered saline (PBS) solutes. Aqueous solutions were dried to different moisture contents by equilibration with saturated salt solutions, or by baking at 95°C. The glass transition temperatures of the dehydrated samples were then measured by differential scanning calorimetry. As expected, Tg increased with decreasing moisture content. For example, in a desiccation medium containing 0.1 M trehalose in PBS, Tg ranged from about 360 K for a completely dry sample to about 220 K at a water mass fraction of 0.4. Addition of polymers to the solutions increased Tg, while addition of penetrating cryoprotectants decreased Tg. Our results provide insight into the relationship between relative humidity, moisture content and glass transition temperature for cell desiccation solutions containing sugars, polymers and penetrating cryoprotectants.
[Mh] Termos MeSH primário: Crioprotetores/química
Polímeros/química
Açúcares/química
Temperatura de Transição
Água/química
[Mh] Termos MeSH secundário: Tampões (Química)
Varredura Diferencial de Calorimetria
Criopreservação/métodos
Dessecação/métodos
Dimetil Sulfóxido/química
Etilenoglicol/química
Ficoll/química
Vidro/química
Modelos Teóricos
Povidona/química
Propilenoglicol/química
Rafinose/química
Soluções/química
Trealose/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL
[Nm] Nome de substância:
0 (Buffers); 0 (Cryoprotective Agents); 0 (Polymers); 0 (Solutions); 0 (Sugars); 059QF0KO0R (Water); 25702-74-3 (Ficoll); 6DC9Q167V3 (Propylene Glycol); B8WCK70T7I (Trehalose); FC72KVT52F (Ethylene Glycol); FZ989GH94E (Povidone); N5O3QU595M (Raffinose); YOW8V9698H (Dimethyl Sulfoxide)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180205
[Lr] Data última revisão:
180205
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180106
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0190713


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[PMID]:28458427
[Au] Autor:Panigrahi PN; Dey S; Sahoo M; Dan A
[Ad] Endereço:Division of Medicine, Indian Veterinary Research Institute, Bareilly, Uttar Pradesh, India.
[Ti] Título:Antiurolithiatic and antioxidant efficacy of pseudostem on ethylene glycol-induced nephrolithiasis in rat.
[So] Source:Indian J Pharmacol;49(1):77-83, 2017 Jan-Feb.
[Is] ISSN:1998-3751
[Cp] País de publicação:India
[La] Idioma:eng
[Ab] Resumo:OBJECTIVE: has been used in the treatment of urolithiasis by the rural people in South India. Therefore, we plan to evaluate its efficacy and possible mechanism of antiurolithiatic effect to rationalize its medicinal use. MATERIALS AND METHODS: Urolithiasis was induced in hyperoxaluric rat model by giving 0.75% ethylene glycol (EG) for 28 days along with 1% ammonium chloride (AC) for the first 14 days. Antiurolithiatic effect of aqueous-ethanol extract of pseudostem (MUSA) was evaluated based on urine and serum biochemistry, microscopy of urine, oxidative/nitrosative indices, kidney calcium content, and histopathology. RESULTS: Administration of EG and AC resulted in increased crystalluria and oxaluria, hypercalciuria, polyuria, crystal deposition in urine, raised serum urea, and creatinine as well as nitric oxide concentration and erythrocytic lipid peroxidation in lithiatic group. However, MUSA treatment significantly restored the impairment in above kidney function test as that of standard treatment, cystone in a dose-dependent manner. CONCLUSIONS: The present findings demonstrate the efficacy of MUSA in EG-induced urolithiasis, which might be mediated through inhibiting various pathways involved in renal calcium oxalate formation, antioxidant effect, and potential to inhibit biochemical markers of renal impairment.
[Mh] Termos MeSH primário: Antioxidantes/farmacologia
Musa/química
Nefrolitíase/tratamento farmacológico
Extratos Vegetais/farmacologia
[Mh] Termos MeSH secundário: Cloreto de Amônio/toxicidade
Animais
Antioxidantes/administração & dosagem
Antioxidantes/isolamento & purificação
Oxalato de Cálcio/metabolismo
Modelos Animais de Doenças
Relação Dose-Resposta a Droga
Etilenoglicol/toxicidade
Índia
Testes de Função Renal
Peroxidação de Lipídeos/efeitos dos fármacos
Masculino
Nefrolitíase/fisiopatologia
Extratos Vegetais/administração & dosagem
Extratos Vegetais/isolamento & purificação
Ratos
Ratos Wistar
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antioxidants); 0 (Plant Extracts); 0 (cystone); 01Q9PC255D (Ammonium Chloride); 2612HC57YE (Calcium Oxalate); FC72KVT52F (Ethylene Glycol)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171229
[Lr] Data última revisão:
171229
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170502
[St] Status:MEDLINE
[do] DOI:10.4103/0253-7613.201026


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[PMID]:29020580
[Au] Autor:Hanouneh M; Chen TK
[Ad] Endereço:Johns Hopkins University, Baltimore, MD mhanoun1@jhmi.edu.
[Ti] Título:Calcium Oxalate Crystals in Ethylene Glycol Toxicity.
[So] Source:N Engl J Med;377(15):1467, 2017 Oct 12.
[Is] ISSN:1533-4406
[Cp] País de publicação:United States
[La] Idioma:eng
[Mh] Termos MeSH primário: Oxalato de Cálcio/urina
Etilenoglicol/envenenamento
[Mh] Termos MeSH secundário: Idoso de 80 Anos ou mais
Etilenoglicol/sangue
Etilenoglicol/urina
Seres Humanos
Masculino
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
2612HC57YE (Calcium Oxalate); FC72KVT52F (Ethylene Glycol)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171019
[Lr] Data última revisão:
171019
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:171012
[St] Status:MEDLINE
[do] DOI:10.1056/NEJMicm1704369


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[PMID]:28631902
[Au] Autor:Pavlyashik GV; Zharikov AY; Kiselev VI
[Ad] Endereço:Altai State Medical University of Minzdrav of Russia, Barnaul, Russia.
[Ti] Título:[Comparative estimation of antilithogenic activity of porcine kidney derived biomedical substance and sodium citrate in experimental urolithiasis].
[So] Source:Urologiia;(2):24-27, 2017 Jun.
[Is] ISSN:1728-2985
[Cp] País de publicação:Russia (Federation)
[La] Idioma:rus
[Ab] Resumo:AIM: to compare the anti-lithogenic activity of biomedical substance derived from freeze-dried porcine kidney and sodium citrate. MATERIALS AND METHODS: The experiments were conducted on Wistar rats divided into three groups of 15 animals each: control group (disease control), comparison group (sodium citrate treatment) and experimental group (treatment with biomedical substance from porcine kidneys). Experimental urolithiasis was modeled using the ethylene glycol model. On every 7th day of the 6 week experiment testing was done calcium and oxalate urine concentration and the activity of marker enzymes of renal epithelial damage: lactate dehydrogenase (LDH), -glutamyl transferase (GGT), and N-acetyl--D-glucosaminidase (NAG). At the end of the experiment, a part of the rats were decapitated and the renal tissue was tested for the oxidant status indicators of (renal thiobarbiturate reactive product content, TBRP, and total prooxidant activity, TPA) and antioxidant enzyme activities: glutathione peroxidase (GPO), superoxide dismutase (SOD) and catalase (CAT). To measure the number and size of calcium deposits formed in the renal papillary area, the Koss histochemical method was used. RESULTS: The experimental findings showed developing oxalate nephrolithiasis in the control group, as indicated by urinary supersaturation of oxalate ion, increased activity of marker enzymes, oxidative stress and the formation of numerous calcium deposits in the renal papillary area. In the comparison group, the 3-week use of sodium citrate contributed to a significant decrease in nephrolithiasis: a 3 to 4-fold decrease in the activity of marker enzymes in the urine, a 3.8-fold increase in the concentration of TBRP, normalization of GPO activity; the number and size of urinary calcium deposits decreased by 3.4 and 1.9 times, respectively. In the experimental group, using biomedical substance led to an even greater therapeutic effect. LDH activity and concentration of TPRP showed 1.9 times and by 26.2% greater decrease than in the comparison group, respectively, SOD and CAT activity almost doubled, there were 3.6 times fewer calcium deposits in the field of view and their mean size was 1.7 times smaller than in the comparison group. CONCLUSION: The study findings showed that the porcine kidney derived biomedical substance provide significantly greater antilithogenic effect than sodium citrate.
[Mh] Termos MeSH primário: Produtos Biológicos/uso terapêutico
Citratos/uso terapêutico
Rim/enzimologia
Nefrolitíase/terapia
[Mh] Termos MeSH secundário: Animais
Catalase
Modelos Animais de Doenças
Etilenoglicol
Liofilização
Glutationa Peroxidase
Rim/química
Nefrolitíase/induzido quimicamente
Ratos
Ratos Wistar
Superóxido Dismutase
Suínos
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biological Products); 0 (Citrates); 1Q73Q2JULR (sodium citrate); EC 1.11.1.6 (Catalase); EC 1.11.1.9 (Glutathione Peroxidase); EC 1.15.1.1 (Superoxide Dismutase); FC72KVT52F (Ethylene Glycol)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171103
[Lr] Data última revisão:
171103
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170621
[St] Status:MEDLINE


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[PMID]:28626142
[Au] Autor:Tanaka M; Kurosawa S
[Ad] Endereço:Health Research Institute, Advanced Industrial Science and Technology (AIST).
[Ti] Título:Surface Modification of PDMS and Plastics with Zwitterionic Polymers.
[So] Source:J Oleo Sci;66(7):699-704, 2017 Jul 01.
[Is] ISSN:1347-3352
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:Surface modification of PDMS, polycarbonate, and acrylic resin was examined using various methacryl polymers bearing sulfobetaine, phosphoryl choline, and oligoethylene glycol units. We have found that zwitterionic polymers are adsorbed on the PDMS surface treated with plasma. The surface of PDMS is stable to keep high hydrophilicity after a month of the modification. On the other hand, one of sulfobetaine polymers showed distinguished adsorption behavior in the case of polycarbonate surface treated with plasma. Suppression effect for nonspecific adsorption of BSA was evaluated using polycarbonate and acrylic resin modified with the polymers. The modified surfaces showed suppression effect for nonspecific adsorption of BSA compared with the surface only treated with plasma.
[Mh] Termos MeSH primário: Dimetilpolisiloxanos/química
Nylons/química
Plásticos/química
Polímeros/química
[Mh] Termos MeSH secundário: Resinas Acrílicas/química
Adsorção
Betaína/análogos & derivados
Etilenoglicol
Interações Hidrofóbicas e Hidrofílicas
Metacrilatos/química
Fosforilcolina
Plasma
Cimento de Policarboxilato/química
Propriedades de Superfície
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Acrylic Resins); 0 (Dimethylpolysiloxanes); 0 (Methacrylates); 0 (Nylons); 0 (Plastics); 0 (Polycarboxylate Cement); 0 (Polymers); 0 (poly(dimethylsiloxane)-polyamide copolymer); 107-73-3 (Phosphorylcholine); 25766-59-0 (polycarbonate); 3SCV180C9W (Betaine); 8CVU22OCJW (sulfobetaine); FC72KVT52F (Ethylene Glycol)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170620
[St] Status:MEDLINE
[do] DOI:10.5650/jos.ess17041


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[PMID]:28626134
[Au] Autor:Tsuchida S; Tenma A; Hamaue N; Murai T; Yoshimura T; Aoki T; Kurosawa T
[Ad] Endereço:Department of Molecular Biosciences, School of Pharmaceutical Sciences, Health Sciences University of Hokkaido.
[Ti] Título:Synthesis and Detection by HPLC of 3-Oxohexadecanoyl-CoA for the Study of Peroxisomal Bifunctional Proteins.
[So] Source:J Oleo Sci;66(7):745-751, 2017 Jul 01.
[Is] ISSN:1347-3352
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:3-oxohexadecanoyl-CoA was synthesized for the study of D-bifunctional protein (EC 4. 2. 1. 107, EC 4. 2. 1. 119, EC 1. 1. 1. n12) and L-bifunctional protein (EC 4. 2. 1. 17, EC 5. 3. 3. 8, EC 1. 1. 1. 35). First, tetradecanal was subjected to the Reformatsky reaction with ethyl bromoacetate, and the product was then converted into ethyl 3-oxohexadecanoate. After acetalization of the 3-oxo ester with ethylene glycol, 3,3-ethlenedioxyhexadecanoic acid was obtained by alkaline hydrolysis. The acid was condensed with coenzyme A (CoA) by the mixed anhydride method, and the resulting CoA ester was deprotected with 4 M HCl to obtain 3-oxohexadecanoyl-CoA. In addition, the behavior of the CoA ester under several conditions of high-performance liquid chromatography (HPLC) was also investigated. We established separation detection of (R)-3-hydroxyhexadecanoyl-CoA, (S)-3-hydroxyhexadecaboyl-CoA, 3-oxohexadecanoyl-CoA, and trans-2-hexadecenoyl-CoA.
[Mh] Termos MeSH primário: Acil Coenzima A/síntese química
Cromatografia Líquida de Alta Pressão
Proteína Multifuncional do Peroxissomo-2
[Mh] Termos MeSH secundário: Acetatos/química
Acil Coenzima A/isolamento & purificação
Aldeídos/química
Etilenoglicol/química
Hidrólise
Fenômenos de Química Orgânica
Oxirredução
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (2-hexadecenoyl-coenzyme A); 0 (Acetates); 0 (Acyl Coenzyme A); 0 (Aldehydes); 35106-50-4 (3-hydroxyhexadecanoyl-coenzyme A); 44AJ2LT15N (tetradecanal); 68-10-0 (bromoacetate); EC 4.2.1.107 (Peroxisomal Multifunctional Protein-2); EC 4.2.1.119 (HSD17B4 protein, human); FC72KVT52F (Ethylene Glycol)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171010
[Lr] Data última revisão:
171010
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170620
[St] Status:MEDLINE
[do] DOI:10.5650/jos.ess16239


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[PMID]:28381775
[Au] Autor:Chaari A; Neji SB; Frikha MH
[Ad] Endereço:Laboratory of Natural Substances, Faculty of Sciences, University of Sfax.
[Ti] Título:Fatty Acid Esterification with Polyols over Acidic Montmorillonite.
[So] Source:J Oleo Sci;66(5):455-461, 2017 May 01.
[Is] ISSN:1347-3352
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:The production of fatty acid esters from stearic, oleic, and palmitic acids and polyols (ethylene glycol and glycerol) was investigated in this work. A series of montmorillonite-based clays catalysts (KSF, KSF/0, KP10, and K10), having different physicochemical properties, were used as acidic catalysts. The influence of the specific surface area and the acidity of the catalysts on the esterification rate were explored. The best catalytic activities were obtained with KSF catalyst. The optimization of various factors on the reaction was also studied, including catalyst concentration, reaction temperature and molar ratio (polyol / fatty acid). The yield rate reached 94% under the optimum conditions and the recovery rate maintained more than 96% after 5 batches.
[Mh] Termos MeSH primário: Bentonita/química
Ésteres/síntese química
Etilenoglicol/química
Ácidos Graxos/síntese química
Glicerol/química
Ácidos Oleicos/química
Ácidos Esteáricos/química
[Mh] Termos MeSH secundário: Silicatos de Alumínio/química
Catálise
Fenômenos Químicos
Esterificação
Temperatura Ambiente
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Aluminum Silicates); 0 (Esters); 0 (Fatty Acids); 0 (Oleic Acids); 0 (Stearic Acids); 1302-78-9 (Bentonite); 1302-87-0 (clay); FC72KVT52F (Ethylene Glycol); PDC6A3C0OX (Glycerol)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170407
[St] Status:MEDLINE
[do] DOI:10.5650/jos.ess16216


  9 / 2284 MEDLINE  
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[PMID]:28376134
[Au] Autor:Tsuji Y; Iwasaki T; Ogata H; Matsumoto Y; Kokeguchi S; Matsumura K; Hyon SH; Shiotani M
[Ad] Endereço:Hanabusa Women's Clinic, Kobe city, Hyogo, Japan. tsuji@hanabusaclinic.com.
[Ti] Título:The Beneficial Effect of Carboxylated Poly-L-Lysine on Cryosurvival of Vitrified Early Stage Embryos.
[So] Source:Cryo Letters;38(1):1-6, 2017 Jan/Feb.
[Is] ISSN:0143-2044
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: In the vitrification of embryos, dimethyl sulfoxide (DMSO) is one of the most effective cryoprotectant agents (CPAs), but cytotoxic effects of DMSO on embryos are well known. Carboxylated poly-L-lysine (CPLL) has been identified as an effective cryoprotectant of cultured cell lines and mammalian oocytes. OBJECTIVE: To evaluate the efficacy and safety of CPLL as a CPA for developmental stage embryos. MATERIALS AND METHODS: Mouse 8-cell embryos and blastocysts were vitrified with ethylene glycol (EG), DMSO/EG, or CPLL/EG and the developmental potency assessed in vitro. RESULTS: In 8-cell embryos, there were no differences between the levels of survival and developmental progress into the blastocyst stage in each solution. At the blastocyst stage, the proportion of dead cells was significantly higher in the EG compared with other solutions. In contrast, there were no differences between the DMSO/EG and CPLL/EG. CONCLUSION: These results indicate that CPLL can be used as a replacement for DMSO in the vitrification of mouse embryos.
[Mh] Termos MeSH primário: Blastocisto/efeitos dos fármacos
Criopreservação/métodos
Crioprotetores/farmacologia
Desenvolvimento Embrionário/efeitos dos fármacos
Polilisina/farmacologia
[Mh] Termos MeSH secundário: Animais
Dimetil Sulfóxido/farmacologia
Etilenoglicol/farmacologia
Feminino
Camundongos
Oócitos/efeitos dos fármacos
Vitrificação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cryoprotective Agents); 25104-18-1 (Polylysine); FC72KVT52F (Ethylene Glycol); YOW8V9698H (Dimethyl Sulfoxide)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170622
[Lr] Data última revisão:
170622
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170405
[St] Status:MEDLINE


  10 / 2284 MEDLINE  
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[PMID]:28349055
[Au] Autor:Sharma I; Khan W; Parveen R; Alam MJ; Ahmad I; Ansari MH; Ahmad S
[Ad] Endereço:Bioactive Natural Product Laboratory, Department of Pharmacognosy and Phytochemistry, Faculty of Pharmacy, Jamia Hamdard, New Delhi 110062, India.
[Ti] Título:Antiurolithiasis Activity of Bioactivity Guided Fraction of against Ethylene Glycol Induced Renal Calculi in Rat.
[So] Source:Biomed Res Int;2017:1969525, 2017.
[Is] ISSN:2314-6141
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Dried rhizome of (pashanbhed) is commonly used as a traditional herbal medicine with a wide range of therapeutic applications including urolithiasis. Aqueous extract of was prepared through maceration followed by decoction (mother extract, 35.9% w/w). Further, polarity based fractions were prepared successively from mother extract which yielded 3.4, 2.9, 5.4, 7.5, and 11.3% w/w of hexane, toluene, dichloromethane (DCM), -butanol, and water fractions, respectively. The in vitro, ex vivo, and real-time antiurolithiasis activity of mother extract and fractions were carried out using aggregation assay in synthetic urine and in rat plasma. The study revealed that DCM fraction has significantly ( < 0.05) greater inhibitory potential than other fractions. Ethylene glycol in drinking water (0.75%, v/v) for 28 days was used for induction of urolithiasis and the curative effects of mother extract and DCM fraction were checked for the level of oxalate, calcium, creatinine, uric acid, and urea of both urine and serum. Treatment with mother extract and DCM fraction at a dose of 185 mg/kg and 7 mg/kg, respectively, in ethylene glycol induced rats resulted in a significant ( < 0.05) decrease in serum and urine markers. Histological study revealed lower number of calcium oxalate deposits with minimum damage in the kidneys of mother extract and DCM fraction treated rats. This result provides a scientific basis for its traditional claims.
[Mh] Termos MeSH primário: Antioxidantes/administração & dosagem
Cálculos Renais/tratamento farmacológico
Extratos Vegetais/administração & dosagem
Saxifragaceae/química
Urolitíase/tratamento farmacológico
[Mh] Termos MeSH secundário: Animais
Antioxidantes/química
Modelos Animais de Doenças
Etilenoglicol/toxicidade
Seres Humanos
Cálculos Renais/induzido quimicamente
Cálculos Renais/patologia
Extratos Vegetais/química
Plantas Medicinais/química
Ratos
Urolitíase/induzido quimicamente
Urolitíase/patologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antioxidants); 0 (Plant Extracts); FC72KVT52F (Ethylene Glycol)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170428
[Lr] Data última revisão:
170428
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170329
[St] Status:MEDLINE
[do] DOI:10.1155/2017/1969525



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