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Pesquisa : D02.033.455.250.700.150 [Categoria DeCS]
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[PMID]:28736835
[Au] Autor:Heineke MH; van der Steen LPE; Korthouwer RM; Hage JJ; Langedijk JPM; Benschop JJ; Bakema JE; Slootstra JW; van Egmond M
[Ad] Endereço:Department of Molecular Cell Biology and Immunology, VU University Medical Center, Amsterdam, The Netherlands.
[Ti] Título:Peptide mimetics of immunoglobulin A (IgA) and FcαRI block IgA-induced human neutrophil activation and migration.
[So] Source:Eur J Immunol;47(10):1835-1845, 2017 Oct.
[Is] ISSN:1521-4141
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:The cross-linking of the IgA Fc receptor (FcαRI) by IgA induces release of the chemoattractant LTB4, thereby recruiting neutrophils in a positive feedback loop. IgA autoantibodies of patients with autoimmune blistering skin diseases therefore induce massive recruitment of neutrophils, resulting in severe tissue damage. To interfere with neutrophil mobilization and reduce disease morbidity, we developed a panel of specific peptides mimicking either IgA or FcαRI sequences. CLIPS technology was used to stabilize three-dimensional structures and to increase peptides' half-life. IgA and FcαRI peptides reduced phagocytosis of IgA-coated beads, as well as IgA-induced ROS production and neutrophil migration in in vitro and ex vivo (human skin) experiments. Since topical application would be the preferential route of administration, Cetomacrogol cream containing an IgA CLIPS peptide was developed. In the presence of a skin permeation enhancer, peptides in this cream were shown to penetrate the skin, while not diffusing systemically. Finally, epitope mapping was used to discover sequences important for binding between IgA and FcαRI. In conclusion, a cream containing IgA or FcαRI peptide mimetics, which block IgA-induced neutrophil activation and migration in the skin may have therapeutic potential for patients with IgA-mediated blistering skin diseases.
[Mh] Termos MeSH primário: Antígenos CD/química
Autoanticorpos/química
Imunoglobulina A/química
Ativação de Neutrófilo/efeitos dos fármacos
Peptidomiméticos/imunologia
Peptidomiméticos/metabolismo
Receptores Fc/química
[Mh] Termos MeSH secundário: Administração Tópica
Antígenos CD/imunologia
Antígenos CD/metabolismo
Autoanticorpos/imunologia
Autoanticorpos/metabolismo
Doenças Autoimunes/terapia
Cetomacrogol/administração & dosagem
Cetomacrogol/química
Mapeamento de Epitopos
Meia-Vida
Seres Humanos
Doenças do Sistema Imune/imunologia
Imunoglobulina A/imunologia
Imunoglobulina A/metabolismo
Transtornos Leucocíticos/imunologia
Leucotrieno B4/metabolismo
Infiltração de Neutrófilos/efeitos dos fármacos
Neutrófilos/efeitos dos fármacos
Neutrófilos/imunologia
Biblioteca de Peptídeos
Peptidomiméticos/química
Fagocitose
Ligação Proteica
Espécies Reativas de Oxigênio/metabolismo
Receptores Fc/imunologia
Receptores Fc/metabolismo
Pele/efeitos dos fármacos
Pele/imunologia
Pele/patologia
Absorção Cutânea
Dermatopatias/imunologia
Dermatopatias/terapia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antigens, CD); 0 (Autoantibodies); 0 (Fc(alpha) receptor); 0 (Immunoglobulin A); 0 (Peptide Library); 0 (Peptidomimetics); 0 (Reactive Oxygen Species); 0 (Receptors, Fc); 1HGW4DR56D (Leukotriene B4); 9004-95-9 (Cetomacrogol)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171105
[Lr] Data última revisão:
171105
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170725
[St] Status:MEDLINE
[do] DOI:10.1002/eji.201646782


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[PMID]:28212635
[Au] Autor:Musazzi UM; Santini B; Selmin F; Marini V; Corsi F; Allevi R; Ferretti AM; Prosperi D; Cilurzo F; Colombo M; Minghetti P
[Ad] Endereço:Department of Pharmaceutilcal Sciences, Università degli Studi di Milano, via G. Colombo, 71, 20133, Milan, Italy.
[Ti] Título:Impact of semi-solid formulations on skin penetration of iron oxide nanoparticles.
[So] Source:J Nanobiotechnology;15(1):14, 2017 Feb 17.
[Is] ISSN:1477-3155
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: This work aimed to provide useful information on the incidence of the choice of formulation in semi-solid preparations of iron-oxide nanoparticles (IONs). The appropriate analytical methods to assess the IONs physical stability and the effect of the semi-solid preparations on IONs human skin penetration were discussed. The physical stability of IONs (D = 31 ± 4 nm; ζ = -65 ± 5 mV) loaded in five semi-solid preparations (0.3% w/v), namely Carbopol gel (CP), hydroxyethyl cellulose gel (HEC), carboxymethylcellulose gel (CMC), cetomacrogol cream (Cet) and cold cream was assessed by combining DLS and low-field pulsed NMR data. The in vitro penetration of IONs was studied using human epidermis or isolated stratum corneum (SC). RESULTS: Reversible and irreversible IONs aggregates were evidenced only in HEC and CMC, respectively. IONs diffused massively through SC preferentially by an intercellular pathway, as assessed by transmission electron microscopy. The semi-solid preparations differently influenced the IONs penetration as compared to the aqueous suspension. Cet cream allowed the highest permeation and the lowest retained amount, while cold cream and CP favored the accumulation into the skin membrane. CONCLUSION: Basic cutaneous semi-solid preparations could be used to administer IONs without affecting their permeation profile if they maintained their physical stability over time. This property is better discriminated by low-field pulsed NMR measurements than the commonly used DLS measurements.
[Mh] Termos MeSH primário: Portadores de Fármacos/química
Compostos Férricos/administração & dosagem
Nanopartículas de Magnetita/administração & dosagem
Absorção Cutânea
[Mh] Termos MeSH secundário: Carboximetilcelulose Sódica/química
Celulose/química
Cetomacrogol/química
Difusão
Estabilidade de Medicamentos
Epiderme/metabolismo
Géis/química
Seres Humanos
Técnicas In Vitro
Microscopia Eletrônica de Transmissão
Tamanho da Partícula
Creme para a Pele/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Drug Carriers); 0 (Ferric Compounds); 0 (Gels); 0 (Magnetite Nanoparticles); 1K09F3G675 (ferric oxide); 9004-34-6 (Cellulose); 9004-95-9 (Cetomacrogol); K679OBS311 (Carboxymethylcellulose Sodium)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170512
[Lr] Data última revisão:
170512
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170219
[St] Status:MEDLINE
[do] DOI:10.1186/s12951-017-0249-6


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[PMID]:28042102
[Au] Autor:van Heugten AJ; Braal CL; Versluijs-Helder M; Vromans H
[Ad] Endereço:Department of Pharmaceutics, Utrecht Institute for Pharmaceutical Sciences, Utrecht University, 3584 CG Utrecht, The Netherlands; Research and Development Department, Tiofarma B.V., Hermanus Boerhaavestraat 1, 3261 ME, Oud-Beijerland, The Netherlands. Electronic address: tvheugten@tiofarma.nl.
[Ti] Título:The influence of cetomacrogol ointment processing on structure: A definitive screening design.
[So] Source:Eur J Pharm Sci;99:279-284, 2017 Mar 01.
[Is] ISSN:1879-0720
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Batch-to-batch variability is a challenge for the industrial scale production of ointments. Therefore the current investigation focussed on identifying and understanding critical process parameters (CPPs) for cetomacrogol ointment. This was evaluated using a definitive screening design (DSD) approach in which fourteen batches were produced under predefined and controlled conditions using the following variables: addition of SiO nanoparticles, mixing speed, cooling rate, heating temperature, container filling temperature and isothermal mixing at the filling temperature. Ointment structure was evaluated using a number of rheological parameters. One of these parameters, yield stress was found to be strongly influenced by filling temperature and mixing speed (p=0.0065 and p=0.0013 respectively). Both significantly affect ointment structure and they also have a significant interaction (p<0.05). Understanding the ointment production process can help in defining a processing window to produce ointment of constant quality.
[Mh] Termos MeSH primário: Cetomacrogol/química
Pomadas/química
[Mh] Termos MeSH secundário: Nanopartículas/química
Controle de Qualidade
Dióxido de Silício/química
Temperatura Ambiente
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Ointments); 7631-86-9 (Silicon Dioxide); 9004-95-9 (Cetomacrogol)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170526
[Lr] Data última revisão:
170526
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170103
[St] Status:MEDLINE


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[PMID]:27499352
[Au] Autor:Thakare M; Israel B; Garner S; Ahmed H; Elder D; Capomacchia A
[Ad] Endereço:a Department of Pharmaceutical and Biomedical Sciences , University of Georgia , Athens , GA , USA.
[Ti] Título:Nonionic surfactant structure on the drug release, formulation and physical properties of ethylcellulose microspheres.
[So] Source:Pharm Dev Technol;22(3):418-425, 2017 May.
[Is] ISSN:1097-9867
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Evaluate the effects of nonionic surfactants Brij 58 and Tween 40 with different structures but similar hydrophilic lipophilic balances (HLBs) on theophylline (TH)-loaded ethylcellulose (EC) microspheres. Microspheres were formulated using ratios of the surfactants with matching HLB values but different chemical-structures at temperatures (22/35 °C) by hydrophobic solvent-emulsion evaporation. Particle size, GMD, drug loading, encapsulation efficiency and dissolution were evaluated. Drug release was determined using the zero- and first-order, Higuchi and Hixson-Crowell models. EC microspheres prepared with surfactant Brij 58 showed discrete, free-flowing spherical particles, solid interiors and increased particle smoothness as temperature increased; those prepared with Tween 40 appeared porous with coarser surface morphology as temperature increased; both were CHLB (Combined HLB) dependent. Dissolution obeyed the Higuchi model drug release for both microspheres prepared with Tween 40 and Brij 58 except for those prepared with Brij 58 at 35 °C, which presented as zero order. The results were ascribed to the different chemical structure of Brij 58 versus Tween 40 and preparation temperature. Surfactant chemical structure is an unreported processing parameter shown here to be important in microsphere formulation. Brij 58 possesses properties unique to its chemical structure that influence pharmaceutical and molecular biopharmaceutical research.
[Mh] Termos MeSH primário: Celulose/análogos & derivados
Portadores de Fármacos/química
Composição de Medicamentos/métodos
Tensoativos/química
Teofilina/administração & dosagem
[Mh] Termos MeSH secundário: Celulose/química
Cetomacrogol/química
Liberação Controlada de Fármacos
Microscopia Eletrônica de Varredura
Microesferas
Tamanho da Partícula
Polissorbatos/química
Solubilidade
Propriedades de Superfície
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Drug Carriers); 0 (Polysorbates); 0 (Surface-Active Agents); 7Z8S9VYZ4B (ethyl cellulose); 9004-34-6 (Cellulose); 9004-95-9 (Cetomacrogol); C137DTR5RG (Theophylline); STI11B5A2X (polysorbate 40)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160809
[St] Status:MEDLINE
[do] DOI:10.1080/10837450.2016.1221431


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[PMID]:26644212
[Au] Autor:Allon I; Touitou E
[Ad] Endereço:Institute of Drug Research, School of Pharmacy, Faculty of Medicine, The Hebrew University of Jerusalem, POB 12065, Jerusalem, 91120, Israel.
[Ti] Título:Scrolls: novel microparticulate systems for enhanced delivery to/across the skin.
[So] Source:Drug Deliv Transl Res;6(1):24-37, 2016 Feb.
[Is] ISSN:2190-3948
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:We describe the scroll system as a new microparticulate structured delivery system for enhanced delivery to/across the skin. The basic components of the scroll system are non-ionic surface active of the type of alkyl polyglycol ethers and a glycol. The unique structures are preserved with addition of various ingredients such as polymers, vegetable oils, pharmaceuticals, and permeation enhancers but are dismissed when amphiphile is withdrawn. The microparticles have a unique scroll structure with multiple "wrapping." Besides enabling superior permeation of drugs into/across the skin, the drugs delivered by scroll systems were more effective in vitro and in vivo compared to controls. Model drugs presented high entrapment capacity in scroll systems. The systems are stable over time and are safe for skin application. In order to form, they require a small number of ingredients, simple preparation method, and are environment friendly. The scroll systems may be new potential tools in the dermal/transdermal pharmaceutical and cosmetic industry.
[Mh] Termos MeSH primário: Portadores de Fármacos
Absorção Cutânea
[Mh] Termos MeSH secundário: Administração Cutânea
Analgésicos/farmacocinética
Animais
Antibacterianos/farmacocinética
Anti-Inflamatórios não Esteroides/farmacocinética
Cetomacrogol/química
Cetomacrogol/farmacologia
Microscopia Crioeletrônica
Portadores de Fármacos/química
Feminino
Seres Humanos
Camundongos
Camundongos Endogâmicos C57BL
Permeabilidade
Absorção Cutânea/efeitos dos fármacos
Tensoativos/química
Tensoativos/farmacologia
Suínos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Analgesics); 0 (Anti-Bacterial Agents); 0 (Anti-Inflammatory Agents, Non-Steroidal); 0 (Drug Carriers); 0 (Surface-Active Agents); 9004-95-9 (Cetomacrogol)
[Em] Mês de entrada:1606
[Cu] Atualização por classe:171111
[Lr] Data última revisão:
171111
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151209
[St] Status:MEDLINE
[do] DOI:10.1007/s13346-015-0264-9


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[PMID]:26341818
[Au] Autor:Chauvet S; Barras A; Boukherroub R; Bouron A
[Ad] Endereço:Univ Grenoble Alpes, iRTSV-LCBM, F-38000 Grenoble, France; CNRS, iRTSV-LCBM, F-38000 Grenoble, France; CEA, iRTSV-LCBM, F-38000 Grenoble, France.
[Ti] Título:Lipid nanocapsules containing the non-ionic surfactant Solutol HS15 inhibit the transport of calcium through hyperforin-activated channels in neuronal cells.
[So] Source:Neuropharmacology;99:726-34, 2015 Dec.
[Is] ISSN:1873-7064
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Hyperforin is described as a natural antidepressant inhibiting the reuptake of neurotransmitters and also activating cation channels. However the blood-brain barrier limits the access to the brain of this biomolecule. To circumvent this problem it was envisaged to encapsulate hyperforin into biomimetic lipid nano-carriers like lipid nanocapsules (LNCs). When testing the safety of 25 nm LNCs it appeared that they strongly blocked hyperforin-activated Ca2+ channels of cultured cortical neurons. This inhibition was due to one of their main component: solutol HS15 (polyoxyethylene-660-12-hydroxy stearate), a non-ionic soluble surfactant. Solutol HS15 rapidly depresses in a concentration-dependent manner the entry of Ca2+ through hyperforin-activated channels without influencing store-operated channels. This effect is mimicked by Brij58 but not by PEG600, indicating that the lipid chain of Solutol HS15 is important in determining its effects on the channels. The inhibition of the Ca2+ fluxes depends on the cellular cholesterol content; it is stronger after depleting cholesterol with methyl-ß-cyclodextrin and is nearly absent on cells cultured in a cholesterol-rich medium. When chronically applied for 24 h, Solutol HS15 slightly up-regulates the entry of Ca2+ through hyperforin-activated channels. Similar observations were made when testing 25 nm lipid nanocapsules containing the surfactant Solutol HS15. Altogether, this study shows that Solutol HS15 perturbs in a cholesterol-dependent manner the activity of some neuronal channels. This is the first demonstration that LNCs containing this surfactant can influence cellular calcium signaling in the brain, a finding that can have important clinical implications.
[Mh] Termos MeSH primário: Sinalização do Cálcio/efeitos dos fármacos
Neurônios/efeitos dos fármacos
Floroglucinol/análogos & derivados
Polietilenoglicóis/administração & dosagem
Ácidos Esteáricos/administração & dosagem
Tensoativos/administração & dosagem
Terpenos/metabolismo
[Mh] Termos MeSH secundário: Animais
Cálcio/metabolismo
Sinalização do Cálcio/fisiologia
Células Cultivadas
Córtex Cerebral/efeitos dos fármacos
Córtex Cerebral/metabolismo
Cetomacrogol/administração & dosagem
Colesterol/metabolismo
Meios de Cultura/química
Relação Dose-Resposta a Droga
Camundongos Endogâmicos C57BL
Nanocápsulas
Neurônios/metabolismo
Floroglucinol/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Culture Media); 0 (Nanocapsules); 0 (Stearic Acids); 0 (Surface-Active Agents); 0 (Terpenes); 30IQX730WE (Polyethylene Glycols); 61909-81-7 (Solutol HS 15); 9004-95-9 (Cetomacrogol); 97C5T2UQ7J (Cholesterol); DHD7FFG6YS (Phloroglucinol); RM741E34FP (hyperforin); SY7Q814VUP (Calcium)
[Em] Mês de entrada:1608
[Cu] Atualização por classe:151121
[Lr] Data última revisão:
151121
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150906
[St] Status:MEDLINE


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[PMID]:26328446
[Au] Autor:Oyafuso MH; Carvalho FC; Chiavacci LA; Gremião MP; Chorilli M
[Ti] Título:Design and Characterization of Silicone and Surfactant Based Systems for Topical Drug Delivery.
[So] Source:J Nanosci Nanotechnol;15(1):817-26, 2015 Jan.
[Is] ISSN:1533-4880
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Nanotechnology offers advantages for new drug delivery design by providing drug targeting while minimizing the side effects. Polyoxyethylene 20 cetyl alcohol (CETETH-20) is a surfactant that may form nanostructured systems, such as liquid crystals, when in contact with water/oil, which are structurally similar to biological membranes and may improve skin interaction. The aim of this study was to develop and characterize CETETH 20-based nanostructured systems by combining CETETH-20 with water and different oily phases, including PEG-12-dimethicone for topical drug administration. The systems were characterized by polarized light microscopy (PLM), small-angle X-ray scattering (SAXS), rheology, texture profile analyses (TPA), in vitro cytotoxicity and histopathological analyses of rabbits' skin. Lamellar, hexagonal and cubic phases were identified and their viscoelastic moduli varied according to each phase. The stiffness of the cubic phase was 3-fold higher and twice more adhesive than the hexagonal phase. The formulations did not affect the normal macrophages cells, neither promoted skin irritation. They were spontaneously obtained by simply mixing the components, which corroborates for an ease scaled-up. These results suggest that systems composed of CETETH 20, PEG-12-dimethicone and water are a promising new approach for designing nanostructured topical drug delivery systems.
[Mh] Termos MeSH primário: Administração Tópica
Portadores de Fármacos
Nanopartículas
Silicones
Tensoativos
[Mh] Termos MeSH secundário: Animais
Linhagem Celular
Sobrevivência Celular/efeitos dos fármacos
Cetomacrogol/administração & dosagem
Cetomacrogol/química
Cetomacrogol/toxicidade
Portadores de Fármacos/administração & dosagem
Portadores de Fármacos/química
Portadores de Fármacos/toxicidade
Emulsões/química
Álcoois Graxos/administração & dosagem
Álcoois Graxos/química
Álcoois Graxos/toxicidade
Masculino
Camundongos
Nanopartículas/administração & dosagem
Nanopartículas/química
Nanopartículas/toxicidade
Ácido Oleico/química
Polietilenoglicóis/administração & dosagem
Polietilenoglicóis/química
Polietilenoglicóis/toxicidade
Coelhos
Silicones/administração & dosagem
Silicones/química
Silicones/toxicidade
Pele/efeitos dos fármacos
Pele/patologia
Testes de Irritação da Pele
Tensoativos/administração & dosagem
Tensoativos/química
Tensoativos/toxicidade
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Drug Carriers); 0 (Emulsions); 0 (Fatty Alcohols); 0 (Silicones); 0 (Surface-Active Agents); 2UMI9U37CP (Oleic Acid); 30IQX730WE (Polyethylene Glycols); 9004-95-9 (Cetomacrogol); 936JST6JCN (cetyl alcohol)
[Em] Mês de entrada:1510
[Cu] Atualização por classe:151119
[Lr] Data última revisão:
151119
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150903
[St] Status:MEDLINE


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[PMID]:26285205
[Au] Autor:Roubalova L; Vosahlikova M; Brejchova J; Sykora J; Rudajev V; Svoboda P
[Ad] Endereço:Department of Biomathematics, Institute of Physiology of the Czech Academy of Sciences, Prague, Czech Republic.
[Ti] Título:High Efficacy but Low Potency of δ-Opioid Receptor-G Protein Coupling in Brij-58-Treated, Low-Density Plasma Membrane Fragments.
[So] Source:PLoS One;10(8):e0135664, 2015.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:PRINCIPAL FINDINGS: HEK293 cells stably expressing PTX-insensitive δ-opioid receptor-Gi1α (C351I) fusion protein were homogenized, treated with low concentrations of non-ionic detergent Brij-58 at 0°C and fractionated by flotation in sucrose density gradient. In optimum range of detergent concentrations (0.025-0.05% w/v), Brij-58-treated, low-density membranes exhibited 2-3-fold higher efficacy of DADLE-stimulated, high-affinity [32P]GTPase and [35S]GTPγS binding than membranes of the same density prepared in the absence of detergent. The potency of agonist DADLE response was significantly decreased. At high detergent concentrations (>0.1%), the functional coupling between δ-opioid receptors and G proteins was completely diminished. The same detergent effects were measured in plasma membranes isolated from PTX-treated cells. Therefore, the effect of Brij-58 on δ-opioid receptor-G protein coupling was not restricted to the covalently bound Gi1α within δ-opioid receptor-Gi1α fusion protein, but it was also valid for PTX-sensitive G proteins of Gi/Go family endogenously expressed in HEK293 cells. Characterization of the direct effect of Brij-58 on the hydrophobic interior of isolated plasma membranes by steady-state anisotropy of diphenylhexatriene (DPH) fluorescence indicated a marked increase of membrane fluidity. The time-resolved analysis of decay of DPH fluorescence by the "wobble in cone" model of DPH motion in the membrane indicated that the exposure to the increasing concentrations of Brij-58 led to a decreased order and higher motional freedom of the dye. SUMMARY: Limited perturbation of plasma membrane integrity by low concentrations of non-ionic detergent Brij-58 results in alteration of δ-OR-G protein coupling. Maximum G protein-response to agonist stimulation (efficacy) is increased; affinity of response (potency) is decreased. The total degradation plasma membrane structure at high detergent concentrations results in diminution of functional coupling between δ-opioid receptors and G proteins.
[Mh] Termos MeSH primário: Membrana Celular/efeitos dos fármacos
Membrana Celular/metabolismo
Cetomacrogol/farmacologia
Detergentes/farmacologia
Receptores Opioides delta/metabolismo
[Mh] Termos MeSH secundário: Membrana Celular/química
Relação Dose-Resposta a Droga
Células HEK293
Seres Humanos
Interações Hidrofóbicas e Hidrofílicas
Toxina Pertussis/toxicidade
Temperatura Ambiente
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Detergents); 0 (Receptors, Opioid, delta); 9004-95-9 (Cetomacrogol); EC 2.4.2.31 (Pertussis Toxin)
[Em] Mês de entrada:1605
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150819
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0135664


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[PMID]:25955505
[Au] Autor:Xu Q; Shen Y; Tang J; Xue MH; Jiang L; Hu X
[Ad] Endereço:†College of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou 225002, China.
[Ti] Título:Electrochemical method assisted immobilization and orientation of myoglobin into biomimetic brij 56 film and its direct electrochemistry study.
[So] Source:ACS Appl Mater Interfaces;7(21):11286-93, 2015 Jun 03.
[Is] ISSN:1944-8252
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:A simple cyclic voltammetric method was applied to assemble and orient a model protein, namely, myoglobin (Mb), into a biocompatible Brij 56 film. Ultraviolet-visible and circular dichroism spectra indicated that Mb in Brij 56 matrix preserved its secondary structure. Fourier transform infrared spectra confirmed the formation of hydrogen bonds between Mb and Brij 56. These hydrogen bonds acted as the electron tunnel to transfer electrons from Mb's active sites to the underlying glassy carbon electrode. Effective direct electron transfer of Mb was realized with the presence of a couple of quasi-reversible and well-defined redox peaks at -310 mV (vs standard calomel electrode) in the studied potential range. The peaks were attributed to the redox couple of heme Fe(II)/Fe(III) of the well-oriented Mb in Brij 56 matrix. The surface coverage and the electron transfer rate (ks) of Mb immobilized into the Brij 56 film was ∼4.9×10(-11) mol cm(-2) and 72.6±3.0 s(-1), respectively. An excellent electrocatalytic response of the immobilized Mb toward nitrite in the absence of electron transfer mediators was observed. These results emphasized that the biomimetic Brij 56 could be used as an attractive material for immobilizing proteins and constructing biosensors.
[Mh] Termos MeSH primário: Materiais Biomiméticos/química
Cetomacrogol/química
Galvanoplastia/métodos
Membranas Artificiais
Mioglobina/química
Mioglobina/ultraestrutura
[Mh] Termos MeSH secundário: Adsorção
Técnicas Biossensoriais/métodos
Condutividade Elétrica
Teste de Materiais
Ligação Proteica
Conformação Proteica
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Membranes, Artificial); 0 (Myoglobin); 9004-95-9 (Cetomacrogol)
[Em] Mês de entrada:1603
[Cu] Atualização por classe:150603
[Lr] Data última revisão:
150603
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150509
[St] Status:MEDLINE
[do] DOI:10.1021/acsami.5b01492


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[PMID]:25909868
[Au] Autor:Krudopp H; Sönnichsen FD; Steffen-Heins A
[Ad] Endereço:Institute of Human Nutrition and Food Science, Kiel University, 24118 Kiel, Germany. Electronic address: info@foodtech.uni-kiel.de.
[Ti] Título:Partitioning of nitroxides in dispersed systems investigated by ultrafiltration, EPR and NMR spectroscopy.
[So] Source:J Colloid Interface Sci;452:15-23, 2015 Aug 15.
[Is] ISSN:1095-7103
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:HYPOTHESIS: The partitioning behavior of paramagnetic nitroxides in dispersed systems can be determined by deconvolution of electron paramagnetic resonance (EPR) spectra giving equivalent results with the validated methods of ultrafiltration techniques (UF) and pulsed-field gradient nuclear magnetic resonance spectroscopy (PFG-NMR). EXPERIMENTS: The partitioning behavior of nitroxides with increasing lipophilicity was investigated in anionic, cationic and nonionic micellar systems and 10 wt% o/w emulsions. Apart from EPR spectra deconvolution, the PFG-NMR was used in micellar solutions as a non-destructive approach, while UF based on separation of very small volume of the aqueous phase. FINDINGS: As a function of their substituent and lipophilicity, the proportions of nitroxides that were solubilized in the micellar or emulsion interface increased with increasing nitroxide lipophilicity for all emulsifier used. Comparing the different approaches, EPR deconvolution and UF revealed comparable nitroxide proportions that were solubilized in the interfaces. Those proportions were higher than found with PFG-NMR. For PFG-NMR self-diffusion experiments the reduced nitroxides were used revealing a high dynamic of hydroxylamines and emulsifiers. Deconvolution of EPR spectra turned out to be the preferred method for measuring the partitioning behavior of paramagnetic molecules as it enables distinguishing between several populations at their individual solubilization sites.
[Mh] Termos MeSH primário: Óxidos N-Cíclicos/química
Emulsificantes/química
Hidroxilamina/química
Água/química
[Mh] Termos MeSH secundário: Cetomacrogol/química
Compostos de Cetrimônio/química
Espectroscopia de Ressonância de Spin Eletrônica
Emulsões
Hidroxilaminas/química
Espectroscopia de Ressonância Magnética
Micelas
Dodecilsulfato de Sódio/química
Ultrafiltração
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cetrimonium Compounds); 0 (Cyclic N-Oxides); 0 (Emulsifying Agents); 0 (Emulsions); 0 (Hydroxylamines); 0 (Micelles); 059QF0KO0R (Water); 2FP81O2L9Z (Hydroxylamine); 368GB5141J (Sodium Dodecyl Sulfate); 9004-95-9 (Cetomacrogol); V55XB6IT82 (TEMPOL-H); VQN7359ICQ (TEMPO); Z7FF1XKL7A (cetrimonium)
[Em] Mês de entrada:1602
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150425
[St] Status:MEDLINE



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