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[PMID]:28866358
[Au] Autor:Imran M; Fazal-E-Habib; Tawab A; Rauf W; Rahman M; Khan QM; Asi MR; Iqbal M
[Ad] Endereço:National Institute for Biotechnology & Genetic Engineering (NIBGE), PO Box 577, Jhang Road, Faisalabad, 38000, Pakistan.
[Ti] Título:LC-MS/MS based method development for the analysis of florfenicol and its application to estimate relative distribution in various tissues of broiler chicken.
[So] Source:J Chromatogr B Analyt Technol Biomed Life Sci;1063:163-173, 2017 Sep 15.
[Is] ISSN:1873-376X
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Florfenicol, a broad spectrum bacteriostatic antibiotic belonging to amphenicol class, is widely used in poultry and livestock for the treatment of various infections. The major metabolite of florfenicol in different animal species is florfenicol amine which is exploited as the marker residue for the determination of florfenicol. Analysis of florfenicol merely by solvent extraction cannot determine the accurate amount of the drug present in incurred tissues (muscle, liver and kidney) of treated birds, as indicated by this study. Thus the methods solely based on solvent extraction may lead to false negative results. A reliable LC-MS/MS based confirmatory method for the analysis of florfenicol and its metabolites in chicken muscle was developed and validated according to the European Union Commission Decision 2002/657/EC. The method was based on acid hydrolysis to liberate non-extractable residues having presumably been covalently bound to tissues, and to convert all the florfenicol residues as well as its metabolites into florfenicol amine. The amine was subsequently recovered with ethyl acetate at pH 10.5, defatted and further cleaned up with dispersive solid phase extraction (dSPE). The LC separation was achieved on reverse phase C-18 column with isocratic elution using acetonitrile/water mobile phase and the analysis was performed on linear ion trap mass spectrometer. Calibration curve was obtained over a concentration range of 25-600µg/kg for chicken muscles. The accuracy values ranged from 84 to 101.4% and the precision values for within day and between days ranged from 1.2-11.7%, respectively. Limit of detection (LOD), limit of quantification (LOD), CCα and CCß values were 0.98, 3.2, 113 and 126µg/kg, respectively. The developed method was highly robust and was further applied to estimate the relative distribution of solvent-extractable against solvent-non-extractable florfenicol drug residues in muscle, liver and kidney samples of broiler chicken after 5days of oral dosing.
[Mh] Termos MeSH primário: Antibacterianos/análise
Galinhas/metabolismo
Cromatografia Líquida/métodos
Resíduos de Drogas/análise
Espectrometria de Massas em Tandem/métodos
Tianfenicol/análogos & derivados
[Mh] Termos MeSH secundário: Animais
Resíduos de Drogas/farmacocinética
Rim/química
Rim/metabolismo
Limite de Detecção
Modelos Lineares
Fígado/química
Fígado/metabolismo
Carne/análise
Músculos/química
Músculos/metabolismo
Reprodutibilidade dos Testes
Tianfenicol/análise
Tianfenicol/farmacocinética
Distribuição Tecidual
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 9J97307Y1H (florfenicol); FLQ7571NPM (Thiamphenicol)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171002
[Lr] Data última revisão:
171002
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170904
[St] Status:MEDLINE


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[PMID]:28838073
[Au] Autor:Bradshaw CS; Jensen JS; Waites KB
[Ad] Endereço:Central Clinical School, Monash University.
[Ti] Título:New Horizons in Mycoplasma genitalium Treatment.
[So] Source:J Infect Dis;216(suppl_2):S412-S419, 2017 Jul 15.
[Is] ISSN:1537-6613
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Mycoplasmagenitalium is an important sexually transmitted pathogen responsible for both male and female genital tract disease. Appreciation of its significance in human disease has been hampered by its slow growth in culture, difficulty in isolating it, and lack of commercial molecular-based tests for rapid detection. Comparatively few in vitro data on antimicrobial susceptibility are available due to the scarcity of clinical isolates and difficulty in performing susceptibility tests to determine minimum inhibitory concentrations for M. genitalium. Antimicrobial agents that inhibit protein synthesis such as macrolides, along with fluoroquinolones that inhibit DNA replication, have been the treatments of choice for M. genitalium infections. Even though international guidelines recommend azithromycin as first-line treatment, rapid spread of macrolide resistance as well as emergence of quinolone resistance has occurred. Increasing rates of treatment failure have resulted in an urgent need for new therapies and renewed interest in other classes such as aminocyclitols, phenicols, and streptogramins as treatment alternatives. Limited data for new investigational antimicrobials such as the ketolide solithromycin suggest that this drug may eventually prove useful in management of some resistant M. genitalium infections, although it is not likely to achieve cure rates >80% in macrolide-resistant strains, in a similar range as recently reported for pristinamycin. However, agents with completely new targets and/or mechanisms that would be less likely to show cross-resistance with currently available drugs may hold the greatest promise. Lefamulin, a pleuromutilin, and new nonquinolone topoisomerase inhibitors are attractive possibilities that require further investigation.
[Mh] Termos MeSH primário: Antibacterianos/uso terapêutico
Descoberta de Drogas/classificação
Infecções por Mycoplasma/diagnóstico
Infecções por Mycoplasma/tratamento farmacológico
[Mh] Termos MeSH secundário: Azitromicina/uso terapêutico
Farmacorresistência Bacteriana
Feminino
Fluoroquinolonas/uso terapêutico
Seres Humanos
Masculino
Testes de Sensibilidade Microbiana
Mycoplasma genitalium
Quinolinas/uso terapêutico
Espectinomicina/uso terapêutico
Estreptograminas/uso terapêutico
Tetraciclinas/uso terapêutico
Tianfenicol/uso terapêutico
Falha de Tratamento
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Fluoroquinolones); 0 (Quinolines); 0 (Streptogramins); 0 (Tetracyclines); 83905-01-5 (Azithromycin); 93AKI1U6QF (Spectinomycin); E66400VT9R (quinoline); FLQ7571NPM (Thiamphenicol)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170906
[Lr] Data última revisão:
170906
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170826
[St] Status:MEDLINE
[do] DOI:10.1093/infdis/jix132


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[PMID]:28690304
[Au] Autor:Minatani T; Sakamoto Y; Nagai H; Goto K
[Ad] Endereço:Gifu Prefectural Research Institute for Health and Environmental Sciences.
[Ti] Título:Determination of Residues of Phenicol Drugs in Ayu (Plecoglossus altivelis) by LC-MS/MS.
[So] Source:Shokuhin Eiseigaku Zasshi;58(3):143-148, 2017.
[Is] ISSN:1882-1006
[Cp] País de publicação:Japan
[La] Idioma:jpn
[Ab] Resumo:An analytical method for the determination of residues of 3 phenicol drugs (chloramphenicol, thiamphenicol and florfenicol) in Ayu (Plecoglossus altivelis) by LC-MS/MS was developed. We used the whole body of Ayu, including the bones and internal organs, in addition to muscle. Phenicols were extracted with 90% acetonitrile and an aliquot of the crude extract was cleaned up on a Florisil column (2 g), followed by defatting with n-hexane. The acetonitrile extract was evaporated and the solvent was replaced with phosphate buffer, then the extract was purified on a hydroxylated styrene-divinylbenzene copolymer column (200 mg). Finally, sample solution was passed through a deproteination cartridge filter with a lipid removal function. Chloramphenicol was quantitated by means of a calibration curve corrected with salogate standard (chloramphenicol-d ) and thiamphenicol and florfenicol were quantitated based on absolute calibration curves. This method was validated in accordance with the notification of the Ministry of Health, Labour and Welfare of Japan. As a result of the validation study, the trueness, repeatability and within-laboratory reproducibility were 85-103, 5-13 and 8-13%, respectively. This method is useful for inspecting residues of 3 phenicol drugs in whole body of Ayu efficiently. Moreover, when chloramphenicol and thiamphenicol are detected by this method, the quantitated value is applicable to decide the compliance of the sample with the specifications and standards of the Food Sanitation Law.
[Mh] Termos MeSH primário: Antibacterianos/análise
Antibacterianos/isolamento & purificação
Cloranfenicol/análise
Cloranfenicol/isolamento & purificação
Cromatografia Líquida/métodos
Resíduos de Drogas/análise
Resíduos de Drogas/isolamento & purificação
Análise de Alimentos/métodos
Osmeriformes/metabolismo
Espectrometria de Massas em Tandem/métodos
Tianfenicol/análogos & derivados
Tianfenicol/análise
Tianfenicol/isolamento & purificação
[Mh] Termos MeSH secundário: Acetonitrilos
Animais
Legislação sobre Alimentos/normas
Extração Líquido-Líquido/métodos
Reprodutibilidade dos Testes
[Pt] Tipo de publicação:JOURNAL ARTICLE; VALIDATION STUDIES
[Nm] Nome de substância:
0 (Acetonitriles); 0 (Anti-Bacterial Agents); 66974FR9Q1 (Chloramphenicol); 9J97307Y1H (florfenicol); FLQ7571NPM (Thiamphenicol); Z072SB282N (acetonitrile)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171016
[Lr] Data última revisão:
171016
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170711
[St] Status:MEDLINE
[do] DOI:10.3358/shokueishi.58.143


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[PMID]:28552968
[Au] Autor:Dorey L; Pelligand L; Cheng Z; Lees P
[Ad] Endereço:Comparative Biological Sciences, Royal Veterinary College, London University, London, United Kingdom.
[Ti] Título:Pharmacokinetic/pharmacodynamic integration and modelling of florfenicol for the pig pneumonia pathogens Actinobacillus pleuropneumoniae and Pasteurella multocida.
[So] Source:PLoS One;12(5):e0177568, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Pharmacokinetic-pharmacodynamic (PK/PD) integration and modelling were used to predict dosage schedules for florfenicol for two pig pneumonia pathogens, Actinobacillus pleuropneumoniae and Pasteurella multocida. Pharmacokinetic data were pooled for two bioequivalent products, pioneer and generic formulations, administered intramuscularly to pigs at a dose rate of 15 mg/kg. Antibacterial potency was determined in vitro as minimum inhibitory concentration (MIC) and Mutant Prevention Concentration in broth and pig serum, for six isolates of each organism. For both organisms and for both serum and broth MICs, average concentration:MIC ratios over 48 h were similar and exceeded 2.5:1 and times greater than MIC exceeded 35 h. From in vitro time-kill curves, PK/PD modelling established serum breakpoint values for the index AUC24h/MIC for three levels of inhibition of growth, bacteriostasis and 3 and 4log10 reductions in bacterial count; means were 25.7, 40.2 and 47.0 h, respectively, for P. multocida and 24.6, 43.8 and 58.6 h for A. pleuropneumoniae. Using these PK and PD data, together with literature MIC distributions, doses for each pathogen were predicted for: (1) bacteriostatic and bactericidal levels of kill; (2) for 50 and 90% target attainment rates (TAR); and (3) for single dosing and daily dosing at steady state. Monte Carlo simulations for 90% TAR predicted single doses to achieve bacteriostatic and bactericidal actions over 48 h of 14.4 and 22.2 mg/kg (P. multocida) and 44.7 and 86.6 mg/kg (A. pleuropneumoniae). For daily doses at steady state, and 90% TAR bacteriostatic and bactericidal actions, dosages of 6.2 and 9.6 mg/kg (P. multocida) and 18.2 and 35.2 mg/kg (A. pleuropneumoniae) were required. PK/PD integration and modelling approaches to dose determination indicate the possibility of tailoring dose to a range of end-points.
[Mh] Termos MeSH primário: Actinobacillus pleuropneumoniae/efeitos dos fármacos
Antibacterianos/farmacologia
Pasteurella multocida/efeitos dos fármacos
Tianfenicol/análogos & derivados
[Mh] Termos MeSH secundário: Animais
Antibacterianos/farmacocinética
Área Sob a Curva
Testes de Sensibilidade Microbiana
Método de Monte Carlo
Suínos
Tianfenicol/farmacocinética
Tianfenicol/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 9J97307Y1H (florfenicol); FLQ7571NPM (Thiamphenicol)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170921
[Lr] Data última revisão:
170921
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170530
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0177568


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[PMID]:28486145
[Au] Autor:Ren X; Wang Z; Gao B; Liu P; Li J
[Ad] Endereço:Key Laboratory for Sustainable Utilization of Marine Fisheries Resources, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, PR China; Function Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laborat
[Ti] Título:Effects of florfenicol on the antioxidant status, detoxification system and biomolecule damage in the swimming crab (Portunus trituberculatus).
[So] Source:Ecotoxicol Environ Saf;143:6-11, 2017 Sep.
[Is] ISSN:1090-2414
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Florfenicol (FLR) is the most commonly used antibacterial agent in aquaculture because of its wide spectrum of activity and few side-effects. We characterized the toxicokinetics of FLR in the swimming crab (Portunus trituberculatus) after intravenous (IV) dosing (20, 40 and 80mg/kg). The results showed that FLR significantly suppressed the antioxidant system of the hepatopancreas. FLR induced transcriptional expression of phase I and phase II detoxification genes (CYP3 and GST, respectively) in a dose- and clearance time-dependent manner and altered the expression of their corresponding enzymes (erythromycin N-demethylase and glutathione S-transferase, respectively). Moreover, FLR induced the transcription of ATP-binding cassette (ABC) transporter subfamily B (ABCB) and subfamily G (ABCG), although ABCG transcription was not induced by FLR at 20mg/kg. Additionally, higher FLR doses caused significant biomolecule damage during the first 48h after delivery. This study will provide an improved understanding of the exact mechanism underlying toxicity in aquatic organisms.
[Mh] Termos MeSH primário: Antibacterianos/toxicidade
Antioxidantes/metabolismo
Braquiúros/efeitos dos fármacos
Braquiúros/enzimologia
Dano ao DNA
Hepatopâncreas/efeitos dos fármacos
Tianfenicol/análogos & derivados
[Mh] Termos MeSH secundário: Subfamília G de Transportadores de Cassetes de Ligação de ATP/genética
Subfamília G de Transportadores de Cassetes de Ligação de ATP/metabolismo
Animais
Aquicultura
Braquiúros/genética
Citocromo P-450 CYP3A/genética
Citocromo P-450 CYP3A/metabolismo
Relação Dose-Resposta a Droga
Glutationa Transferase/genética
Glutationa Transferase/metabolismo
Hepatopâncreas/enzimologia
Inativação Metabólica
Peroxidação de Lipídeos/efeitos dos fármacos
Frutos do Mar
Natação
Tianfenicol/uso terapêutico
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (ATP Binding Cassette Transporter, Sub-Family G); 0 (Anti-Bacterial Agents); 0 (Antioxidants); 9J97307Y1H (florfenicol); EC 1.14.14.1 (Cytochrome P-450 CYP3A); EC 2.5.1.18 (Glutathione Transferase); FLQ7571NPM (Thiamphenicol)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171023
[Lr] Data última revisão:
171023
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170510
[St] Status:MEDLINE


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[PMID]:28284617
[Au] Autor:Tamang MD; Moon DC; Kim SR; Kang HY; Lee K; Nam HM; Jang GC; Lee HS; Jung SC; Lim SK
[Ad] Endereço:Bacterial Disease Division, Animal and Plant Quarantine Agency, 177 Hyeoksin 8-ro, Gimcheon-si, Gyeongsangbuk-do, 39660, Republic of Korea.
[Ti] Título:Detection of novel oxazolidinone and phenicol resistance gene optrA in enterococcal isolates from food animals and animal carcasses.
[So] Source:Vet Microbiol;201:252-256, 2017 Mar.
[Is] ISSN:1873-2542
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Altogether 7720 Enterococcus faecalis and 3939 E. faecium isolated from food animals and animal carcasses during 2003-2014 in Korea were investigated to determine if linezolid-resistant (LR) enterococci (≥8µg/ml) are present. Overall, 12 E. faecalis and 27 E. faecium recovered from chickens (n=32), pigs (n=6), and cattle (n=1) were resistant to linezolid and were further characterized using molecular methods Most LR isolates were also resistant to chloramphenicol (97.44%) and florfenicol (92.31%). Molecular analysis showed no mutations in the 23S ribosomal RNA and in the ribosomal protein L3. The optrA gene was found in 89.74% of the LR enterococci, including 12 E. faecalis and 23 E. faecium isolates. Among them, 30 optrA-positive isolates co-carried phenicol exporter gene fexA. Seven LR E. faecium isolates had Asn130Lys mutations in the ribosomal protein L4, of which six also carried optrA gene. None of the isolates carried the mutliresistance gene cfr. Transfer of optrA gene was observed in 16 of the 35 optrA-positive isolates by conjugation. Pulsed-field gel electrophoresis demonstrated that the vast majority of Enterococcus strains carrying optrA gene were genetically heterogeneous. Multi-locus sequence typing revealed eight novel Sequence types among E. faecalis and E. faecium strains. To our knowledge, this is the first report of optrA gene in isolates from cattle and animal carcasses. This is also the first report of optrA gene in Korea. Active surveillance of optrA in enterococci is urgently warranted.
[Mh] Termos MeSH primário: Antibacterianos/farmacologia
Galinhas/microbiologia
Farmacorresistência Bacteriana/genética
Enterococcus/efeitos dos fármacos
Microbiologia de Alimentos
Infecções por Bactérias Gram-Positivas/veterinária
[Mh] Termos MeSH secundário: Animais
Técnicas de Tipagem Bacteriana/veterinária
Bovinos
Cloranfenicol/farmacologia
Eletroforese em Gel de Campo Pulsado/veterinária
Enterococcus/genética
Enterococcus/isolamento & purificação
Enterococcus faecalis/efeitos dos fármacos
Enterococcus faecalis/genética
Enterococcus faecalis/isolamento & purificação
Fezes/microbiologia
Infecções por Bactérias Gram-Positivas/microbiologia
Linezolida/farmacologia
Tipagem de Sequências Multilocus/veterinária
Mutação
Oxazolidinonas/farmacologia
República da Coreia
Suínos
Tianfenicol/análogos & derivados
Tianfenicol/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Oxazolidinones); 66974FR9Q1 (Chloramphenicol); 9J97307Y1H (florfenicol); FLQ7571NPM (Thiamphenicol); ISQ9I6J12J (Linezolid)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170927
[Lr] Data última revisão:
170927
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170313
[St] Status:MEDLINE


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[PMID]:28257901
[Au] Autor:Wang M; Zhang Y; Guo P
[Ad] Endereço:Department of Environmental Science and Engineering, College of Chemical Engineering, Huaqiao University, Xiamen, Fujian 361021, China; Institute of Environmental and Resources Technology, Huaqiao University, Xiamen 361021, China.
[Ti] Título:Effect of florfenicol and thiamphenicol exposure on the photosynthesis and antioxidant system of Microcystis flos-aquae.
[So] Source:Aquat Toxicol;186:67-76, 2017 May.
[Is] ISSN:1879-1514
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Florfenicol (FF) and thiamphenicol (TAP) are two typical pharmaceuticals used widely as therapeutica antibiotic agents in aquaculture. However, little is known about the potential adverse effects of these two antibiotics on non-target organisms in the aquatic ecosystem. In this study we investigated the effects of FF and TAP on photosynthesis and the antioxidant system of the cyanobacteria Microcystis flos-aquae. Over a concentration range of 0.001-1µg/L, the results showed that both FF and TAP significantly increased the chlorophyll a content of M. flos-aquae, while the superoxide dismutase (SOD) activity, catalase (CAT) activity and the levels of malondialdehyde (MDA) changed slightly. In contrast, the chlorophyll a content of M. flos-aqua was significantly inhibited (p<0.01) at high concentrations (>1µg/L) of FF and TAP, reaching a 46% inhibition level at 50µg/L FF and 56% inhibition at 100µg/L TAP. At the same time, the activities of SOD and CAT along with MDA content also increased significantly (p<0.01), indicating that the high concentrations of both FF and TAP led to oxidative stress in the algae. In addition, the M. flos-aquae fluorescence parameters (Fv/Fm, Fv/Fo, alpha, ETRmax and Ik) increased with increasing concentration of both FF and TAP, which may be the result of the increasing photoprotection capacity.
[Mh] Termos MeSH primário: Antioxidantes/metabolismo
Exposição Ambiental/análise
Microcystis/metabolismo
Fotossíntese/efeitos dos fármacos
Tianfenicol/análogos & derivados
Tianfenicol/toxicidade
[Mh] Termos MeSH secundário: Proteínas de Algas/metabolismo
Catalase/metabolismo
Clorofila/metabolismo
Peroxidação de Lipídeos/efeitos dos fármacos
Peróxidos Lipídicos/metabolismo
Malondialdeído/metabolismo
Microcystis/efeitos dos fármacos
Estresse Oxidativo/efeitos dos fármacos
Superóxido Dismutase/metabolismo
Poluentes Químicos da Água/toxicidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Algal Proteins); 0 (Antioxidants); 0 (Lipid Peroxides); 0 (Water Pollutants, Chemical); 1406-65-1 (Chlorophyll); 4Y8F71G49Q (Malondialdehyde); 9J97307Y1H (florfenicol); EC 1.11.1.6 (Catalase); EC 1.15.1.1 (Superoxide Dismutase); FLQ7571NPM (Thiamphenicol); YF5Q9EJC8Y (chlorophyll a)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170606
[Lr] Data última revisão:
170606
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170305
[St] Status:MEDLINE


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[PMID]:28199699
[Au] Autor:Verner-Jeffreys DW; Brazier T; Perez RY; Ryder D; Card RM; Welch TJ; Hoare R; Ngo T; McLaren N; Ellis R; Bartie KL; Feist SW; Rowe WMP; Adams A; Thompson KD
[Ad] Endereço:Cefas Weymouth laboratory, The Nothe, Barrack Road, Weymouth DT4 8UB, UK.
[Ti] Título:Detection of the florfenicol resistance gene floR in Chryseobacterium isolates from rainbow trout. Exception to the general rule?
[So] Source:FEMS Microbiol Ecol;93(4), 2017 Apr 01.
[Is] ISSN:1574-6941
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Bacteria from the family Flavobacteriaceae often show low susceptibility to antibiotics. With the exception of two Chryseobacterium spp. isolates that were positive for the florfenicol resistance gene floR, no clinical resistance genes were identified by microarray in 36 Flavobacteriaceae isolates from salmonid fish that could grow in ≥ 4 mg/L florfenicol. Whole genome sequence analysis of the floR positive isolates revealed the presence of a region that contained the antimicrobial resistance genes floR, a tet(X) tetracycline resistance gene, a streptothricin resistance gene and a chloramphenicol acetyltransferase gene. In silico analysis of 377 published genomes for Flavobacteriaceae isolates from a range of sources confirmed that well-characterised resistance gene cassettes were not widely distributed in bacteria from this group. Efflux pump-mediated decreased susceptibility to a range of antimicrobials was confirmed in both floR positive isolates using an efflux pump inhibitor (phenylalanine-arginine ß-naphthylamide) assay. The floR isolates possessed putative virulence factors, including production of siderophores and haemolysins, and were mildly pathogenic in rainbow trout. Results support the suggestion that, despite the detection of floR, susceptibility to antimicrobials in Flavobacteriaceae is mostly mediated via intrinsic mechanisms rather than the horizontally acquired resistance genes more normally associated with Gram-negative bacterial pathogens such as Enterobacteriaceae.
[Mh] Termos MeSH primário: Antibacterianos/farmacologia
Proteínas de Bactérias/genética
Chryseobacterium/efeitos dos fármacos
Chryseobacterium/genética
Oncorhynchus mykiss/microbiologia
Tianfenicol/análogos & derivados
[Mh] Termos MeSH secundário: Acetiltransferases/genética
Animais
Proteínas de Transporte/antagonistas & inibidores
Proteínas de Transporte/genética
Cloranfenicol O-Acetiltransferase/genética
Chryseobacterium/isolamento & purificação
Genoma Bacteriano/genética
Proteínas Hemolisinas/biossíntese
Seres Humanos
Testes de Sensibilidade Microbiana
Fenilalanina/análogos & derivados
Fenilalanina/farmacologia
Reação em Cadeia da Polimerase
Sideróforos/biossíntese
Resistência a Tetraciclina/genética
Tianfenicol/farmacologia
Fatores de Virulência/biossíntese
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Bacterial Proteins); 0 (Carrier Proteins); 0 (Hemolysin Proteins); 0 (Siderophores); 0 (Virulence Factors); 47E5O17Y3R (Phenylalanine); 740-57-8 (phenylalanine-beta-naphthylamide); 9J97307Y1H (florfenicol); EC 2.3.1.- (Acetyltransferases); EC 2.3.1.- (streptothricin acetyltransferase); EC 2.3.1.28 (Chloramphenicol O-Acetyltransferase); FLQ7571NPM (Thiamphenicol)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171003
[Lr] Data última revisão:
171003
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170216
[St] Status:MEDLINE
[do] DOI:10.1093/femsec/fix015


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[PMID]:28125301
[Au] Autor:Pozniak B; Pawlowski P; Paslawska U; Grabowski T; Suszko A; Lis M; Switala M
[Ad] Endereço:a Department of Biochemistry, Pharmacology and Toxicology, Faculty of Veterinary Medicine , Wroclaw University of Environmental and Life Sciences , Wroclaw , Poland.
[Ti] Título:The influence of rapid growth in broilers on florfenicol pharmacokinetics - allometric modelling of the pharmacokinetic and haemodynamic parameters.
[So] Source:Br Poult Sci;58(2):184-191, 2017 Apr.
[Is] ISSN:1466-1799
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:1. The aim of this study was to determine if the pharmacokinetics (PK) of florfenicol (FF) undergo age-dependent changes in broilers. Since drug elimination depends on cardiovascular functions, a haemodynamic study was performed in parallel. 2. Broilers of 0.68, 1.27, 2.45 and 5.13 kg were administered FF in a single intravenous dose of 30 mg/kg body weight. Plasma drug concentrations were determined using high-performance liquid chromatography and PK parameters were calculated using a non-compartmental model. Echocardiography was used to measure haemodynamic functions. 3. During growth, the area under the drug concentration-time curve (AUC ) increased from 25.7 ± 2.9 to 39.0 ± 8.0 mg h/l. Total body clearance (Cl ) gradually decreased from 1.19 ± 0.14 to 0.80 ± 0.15 l/h/kg. Elimination half-life increased from 0.73 ± 0.08 to 1.07 ± 0.07 h, whereas volume of distribution (V ) remained unchanged. Haemodynamic measurements revealed an increase in cardiac output, from 495 ± 65 to 1303 ± 306 ml/min, in the respective body weight groups. 4. Allometric models for PK and haemodynamic parameters were developed and validated. All models proved to be statistically significant; however, only models for Cl and V met stringent validation criteria. Model for Cl was used to calculate an optimal dose for a given age group that provides uniform AUC . 5. Age-dependent change in FF kinetics may cause variability in therapeutic response under clinical conditions. A novel approach to the dosing protocol was proposed as a means of optimising therapeutic efficacy.
[Mh] Termos MeSH primário: Galinhas/metabolismo
Hemodinâmica
Modelos Biológicos
Tianfenicol/análogos & derivados
[Mh] Termos MeSH secundário: Administração Intravenosa/veterinária
Fatores Etários
Animais
Antibacterianos/farmacocinética
Peso Corporal
Galinhas/crescimento & desenvolvimento
Ecocardiografia/veterinária
Masculino
Distribuição Aleatória
Tianfenicol/farmacocinética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 9J97307Y1H (florfenicol); FLQ7571NPM (Thiamphenicol)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170414
[Lr] Data última revisão:
170414
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170127
[St] Status:MEDLINE
[do] DOI:10.1080/00071668.2016.1261994


  10 / 751 MEDLINE  
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[PMID]:27915584
[Au] Autor:Cornejo J; Pokrant E; Riquelme R; Briceño C; Maddaleno A; Araya-Jordán C; San Martin B
[Ad] Endereço:a Preventive Medicine Department, Faculty of Veterinary and Animal Sciences , University of Chile , Santiago , Chile.
[Ti] Título:Single-laboratory validation of an LC-MS/MS method for determining florfenicol (FF) and florfenicol amine (FFA) residues in chicken feathers and application to a residue-depletion study.
[So] Source:Food Addit Contam Part A Chem Anal Control Expo Risk Assess;34(4):469-476, 2017 Apr.
[Is] ISSN:1944-0057
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A suitable analytical method is required to study the behaviour of florfenicol (FF) and its metabolite florfenicol amine (FFA) in broiler's feathers. An LC-MS/MS method was developed, assessed and intra-laboratory-validated for FF and FFA analyses. We chose cloramphenicol-d as an internal standard, acetone as a solvent for the extraction of the analytes and dichloromethane for the clean-up. Through LC-MS/MS analysis, we established a detection limit of 20 µg kg , as well as calculated quantification limits of 24.4 and 24.5 µg kg for FF and FFA, respectively. Validation parameters such as linearity, recovery and precision were calculated following Commission Decision 2002/657/EC. For linearity, all standard curves showed a standard coefficient greater than 0.99. Recoveries ranged from 99% to 102% for all studied concentrations. The results show that this analytical method is precise and reliable. For the depletion study, 64 Ross 308 broilers were treated with a therapeutic dosage of 10% FF during 5 consecutive days and their feathers were then analysed. Samples were drawn on days 5, 10, 15, 20, 25, 30, 35 and 40 post-treatments. As for the control group, 16 broiler chickens were raised under the same regime. Throughout the whole study, the detected concentrations of FF and FFA in feather samples were above 100 µg kg . In fact, even on day 30 post-treatment we detected concentrations of 221.8 and 28.8 µg kg for FF and FFA, respectively. Based on these results, we conclude that these analytes will persist for a long time and will deplete slowly in feathers of treated broiler chickens.
[Mh] Termos MeSH primário: Antibacterianos/análise
Cromatografia Líquida/normas
Resíduos de Drogas/análise
Plumas/química
Espectrometria de Massas em Tandem/normas
Tianfenicol/análogos & derivados
[Mh] Termos MeSH secundário: Acetona/química
Animais
Antibacterianos/administração & dosagem
Antibacterianos/farmacocinética
Galinhas
Cloranfenicol/análise
Resíduos de Drogas/farmacocinética
Guias como Assunto
Limite de Detecção
Cloreto de Metileno/química
Padrões de Referência
Solventes/química
Tianfenicol/administração & dosagem
Tianfenicol/análise
Tianfenicol/farmacocinética
[Pt] Tipo de publicação:JOURNAL ARTICLE; VALIDATION STUDIES
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Solvents); 1364PS73AF (Acetone); 588X2YUY0A (Methylene Chloride); 66974FR9Q1 (Chloramphenicol); 76639-93-5 (florfenicol amine); 9J97307Y1H (florfenicol); FLQ7571NPM (Thiamphenicol)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170308
[Lr] Data última revisão:
170308
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161206
[St] Status:MEDLINE
[do] DOI:10.1080/19440049.2016.1267876



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