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Pesquisa : D02.033.623 [Categoria DeCS]
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[PMID]:29339722
[Au] Autor:Sousa DZ; Visser M; van Gelder AH; Boeren S; Pieterse MM; Pinkse MWH; Verhaert PDEM; Vogt C; Franke S; Kümmel S; Stams AJM
[Ad] Endereço:Laboratory of Microbiology, Wageningen University & Research, Stippeneng 4, 6708 WE, Wageningen, The Netherlands.
[Ti] Título:The deep-subsurface sulfate reducer Desulfotomaculum kuznetsovii employs two methanol-degrading pathways.
[So] Source:Nat Commun;9(1):239, 2018 01 16.
[Is] ISSN:2041-1723
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Methanol is generally metabolized through a pathway initiated by a cobalamine-containing methanol methyltransferase by anaerobic methylotrophs (such as methanogens and acetogens), or through oxidation to formaldehyde using a methanol dehydrogenase by aerobes. Methanol is an important substrate in deep-subsurface environments, where thermophilic sulfate-reducing bacteria of the genus Desulfotomaculum have key roles. Here, we study the methanol metabolism of Desulfotomaculum kuznetsovii strain 17 , isolated from a 3000-m deep geothermal water reservoir. We use proteomics to analyze cells grown with methanol and sulfate in the presence and absence of cobalt and vitamin B12. The results indicate the presence of two methanol-degrading pathways in D. kuznetsovii, a cobalt-dependent methanol methyltransferase and a cobalt-independent methanol dehydrogenase, which is further confirmed by stable isotope fractionation. This is the first report of a microorganism utilizing two distinct methanol conversion pathways. We hypothesize that this gives D. kuznetsovii a competitive advantage in its natural environment.
[Mh] Termos MeSH primário: Álcool Desidrogenase/metabolismo
Proteínas de Bactérias/metabolismo
Desulfotomaculum/enzimologia
Redes e Vias Metabólicas/genética
Metanol/metabolismo
Metiltransferases/metabolismo
[Mh] Termos MeSH secundário: Álcool Desidrogenase/genética
Proteínas de Bactérias/genética
Cobalto/metabolismo
Cobalto/farmacologia
Meios de Cultura/química
Desulfotomaculum/genética
Expressão Gênica
Perfilação da Expressão Gênica
Hidrólise
Metiltransferases/genética
Oxirredução
Filogenia
Proteômica/métodos
Vitamina B 12/metabolismo
Vitamina B 12/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Culture Media); 3G0H8C9362 (Cobalt); EC 1.1.1.1 (Alcohol Dehydrogenase); EC 2.1.1.- (Methyltransferases); EVS87XF13W (cobaltous chloride); P6YC3EG204 (Vitamin B 12); Y4S76JWI15 (Methanol)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180307
[Lr] Data última revisão:
180307
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180118
[St] Status:MEDLINE
[do] DOI:10.1038/s41467-017-02518-9


  2 / 14329 MEDLINE  
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[PMID]:29329094
[Au] Autor:Sentkowska A; Pyrzynska K
[Ad] Endereço:University of Warsaw, Heavy Ion Laboratory, Pasteura 5A, 02-093 Warsaw, Poland. Electronic address: sentkowska@slcj.uw.edu.pl.
[Ti] Título:Hydrophilic interaction liquid chromatography in the speciation analysis of selenium.
[So] Source:J Chromatogr B Analyt Technol Biomed Life Sci;1074-1075:8-15, 2018 Feb 01.
[Is] ISSN:1873-376X
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The hydrophilic interaction liquid chromatography (HILIC) coupled to mass spectrometry was employed to study retention behavior of selected selenium compounds using two different HILIC stationary phases: silica and zwitterionic. Two organic solvents - acetonitrile and methanol - were compared as a component of mobile phase. Separation parameters such as a content of organic modifier, the eluent pH and inorganic buffer concentration were investigated. Based on all observations, methanol seems to be beneficial for the separation of studied compounds. The optimal HILIC separation method involved silica column and eluent composed of 85% MeOH and CH COONH (8 mM, pH 7) was compared to RP method in terms of time of the single run, the separation efficiency and limit of detection.
[Mh] Termos MeSH primário: Cromatografia Líquida/métodos
Compostos de Selênio
Selênio
[Mh] Termos MeSH secundário: Acetonitrilos/química
Interações Hidrofóbicas e Hidrofílicas
Metanol/química
Cebolas/química
Extratos Vegetais/química
Folhas de Planta/química
Selênio/análise
Selênio/química
Selênio/classificação
Compostos de Selênio/análise
Compostos de Selênio/química
Compostos de Selênio/classificação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Acetonitriles); 0 (Plant Extracts); 0 (Selenium Compounds); H6241UJ22B (Selenium); Y4S76JWI15 (Methanol); Z072SB282N (acetonitrile)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180305
[Lr] Data última revisão:
180305
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180113
[St] Status:MEDLINE


  3 / 14329 MEDLINE  
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[PMID]:29449464
[Au] Autor:Sushkevich VL; Palagin D; Ranocchiari M; van Bokhoven JA
[Ad] Endereço:Laboratory for Catalysis and Sustainable Chemistry, Paul Scherrer Institut, 5232 Villigen, Switzerland. vitaly.sushkevich@psi.ch jeroen.vanbokhoven@chem.ethz.ch.
[Ti] Título:Response to Comment on "Selective anaerobic oxidation of methane enables direct synthesis of methanol".
[So] Source:Science;359(6377), 2018 02 16.
[Is] ISSN:1095-9203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Labinger argues that stepwise reaction of methane with water to produce methanol and hydrogen will never be commercially feasible because of its substoichiometric basis with respect to the active site and the requirement of a large temperature swing. This comment is not touching any new ground, beyond describing the thermodynamic feasibility, thermal cycling, and the role of water as discussed previously. Most important, it does not have a solid numerical basis.
[Mh] Termos MeSH primário: Metano/química
Metanol/síntese química
[Mh] Termos MeSH secundário: Oxirredução
Temperatura Ambiente
Termodinâmica
[Pt] Tipo de publicação:JOURNAL ARTICLE; COMMENT
[Nm] Nome de substância:
OP0UW79H66 (Methane); Y4S76JWI15 (Methanol)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180227
[Lr] Data última revisão:
180227
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180217
[St] Status:MEDLINE


  4 / 14329 MEDLINE  
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[PMID]:29402248
[Au] Autor:Al-Afifi NA; Alabsi AM; Bakri MM; Ramanathan A
[Ad] Endereço:Department of Oral and Craniofacial Sciences, Faculty of Dentistry, University of Malaya, 50603, Kuala Lumpur, Malaysia.
[Ti] Título:Acute and sub-acute oral toxicity of Dracaena cinnabari resin methanol extract in rats.
[So] Source:BMC Complement Altern Med;18(1):50, 2018 Feb 05.
[Is] ISSN:1472-6882
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Dracaena cinnabari (DC) is a perennial tree that located on the Southern coast of Yemen native to the Socotra Island. This tree produces a deep red resin known as the Dragon's blood, the Twobrother's Blood or Damm Alakhwain. The current study performed to evaluate the safety of the DC resin methanol extract after a single or 28 consecutive daily oral administrations. METHODS: In assessing the safety of DC resin methanol extract, acute and sub-acute oral toxicity tests performed following OECD guidelines 423 and 407, respectively, with slight modifications. In acute oral toxicity test, DC resin methanol extract administered to female Sprague Dawley rats by oral gavage at a single dose of 300 and 2000 mg/kg body weight. Rats observed for toxic signs for 14 days. In sub-acute oral toxicity test, DC resin methanol extract administered to the rats by oral gavage at 500, 1000, and 1500 mg/kg body weight daily up to 28 days to male and female Spradgue Dawley rats. The control and high dose in satellite groups were also maintained and handled as the previous groups to determine the late onset toxicity of DC resin methanol extract. At the end of each test, hematological and biochemical analysis of the collected blood were performed as well as gross and microscopic pathology. RESULTS: In acute oral toxicity, no treatment-related death or toxic signs were observed. It revealed that the DC resin methanol extract could be well tolerated up to the dose 2000 mg/kg body weight and could be classified as Category 5. The sub-acute test observations indicated that there are no treatment-related changes up to the high dose level compared to the control. Food consumption, body weight, organ weight, hematological parameters, biochemical parameters and histopathological examination (liver, kidney, heart, spleen and lung) revealed no abnormalities. Water intake was significantly higher in the DC resin methanol extract treated groups compared to the control. CONCLUSION: This study demonstrates tolerability of DC resin methanol extract administered daily for 28 days up to 1500 mg/kg dose.
[Mh] Termos MeSH primário: Dracaena/química
Resinas Vegetais/toxicidade
[Mh] Termos MeSH secundário: Administração Oral
Animais
Peso Corporal/efeitos dos fármacos
Ingestão de Líquidos/efeitos dos fármacos
Ingestão de Alimentos/efeitos dos fármacos
Feminino
Coração/efeitos dos fármacos
Rim/efeitos dos fármacos
Masculino
Metanol
Tamanho do Órgão/efeitos dos fármacos
Ratos
Ratos Sprague-Dawley
Resinas Vegetais/administração & dosagem
Testes de Toxicidade Aguda
Testes de Toxicidade Subcrônica
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Resins, Plant); Y4S76JWI15 (Methanol)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180226
[Lr] Data última revisão:
180226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180207
[St] Status:MEDLINE
[do] DOI:10.1186/s12906-018-2110-3


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[PMID]:28458081
[Au] Autor:van Putten RJ; van der Waal JC; de Jong E; Heeres HJ
[Ad] Endereço:Avantium Chemicals B.V., Zekeringstraat 29, 1014 BV Amsterdam, The Netherlands. Electronic address: robert-jan.vanputten@avantium.com.
[Ti] Título:Reactivity studies in water on the acid-catalysed dehydration of psicose compared to other ketohexoses into 5-hydroxymethylfurfural.
[So] Source:Carbohydr Res;446-447:1-6, 2017 Jun 29.
[Is] ISSN:1873-426X
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The conversion of the four possible ketohexoses (fructose, tagatose, sorbose and psicose) into 5-hydroxymethylfurfural (HMF) was explored in water using sulphuric acid as the catalyst (33 mM H SO , 120 °C). Significant differences in reactivity were observed and tagatose (48% conversion after 75 min) and psicose (35% conversion after 75 min) were clearly more reactive than fructose and sorbose (around 20% conversion after 75 min). The selectivity to HMF was found to be higher for fructose and psicose than for tagatose and sorbose. 2-Hydroxyacetylfuran (HAF) was shown to be a by-product for mainly sorbose and tagatose (as high as 2% yield). The results indicate that the relative orientation of the hydroxyl groups on C3 and C4 has a major effect on the reactivity and selectivity. This suggests that the dehydration towards HMF takes place via a mechanism with cyclic intermediates in which the C3C4 bond is fixed in a ring structure. A reaction mechanism involving a bicyclic structure is proposed to explain the formation of HAF. The reactivity of the sugars was significantly lower in water than previously observed in methanol.
[Mh] Termos MeSH primário: Frutose/química
Furaldeído/análogos & derivados
Água/química
[Mh] Termos MeSH secundário: Catálise
Furaldeído/química
Concentração de Íons de Hidrogênio
Metanol/química
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
059QF0KO0R (Water); 23140-52-5 (psicose); 30237-26-4 (Fructose); 70ETD81LF0 (5-hydroxymethylfurfural); DJ1HGI319P (Furaldehyde); Y4S76JWI15 (Methanol)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180219
[Lr] Data última revisão:
180219
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170502
[St] Status:MEDLINE


  6 / 14329 MEDLINE  
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[PMID]:29364598
[Au] Autor:Doronina NV; Kaparulina EN; Trotsenko YA
[Ti] Título:Emended Description of Methylovorus glucosotrophus Govorukhina and Trotsenko 1991.
[So] Source:Mikrobiologiia;85(5):506-511, 2016 Sep.
[Is] ISSN:0026-3656
[Cp] País de publicação:Russia (Federation)
[La] Idioma:eng
[Ab] Resumo:Phylogeneticanalysis based,on comparison of the 16S rRNA gene sequences in combination with comparative analysis of physiological, biochemical, and chemotaxonomic characteristics and DNA-DNA hybridization revealed that "Methylobacillusfructoseoxidans" 34 (VKM B-1609 = DSM 5897 and-Methylov- orus glucosotrophus 6B 1T (ATCC 49758T = DSM 6874T = VKM B- 1745T = NCIMB 13222 ) belong to the same Methylovorus species. Extended description of the limited facultative methylotroph Methylovorus gluco- sotrophus is proposed, which includes the fructose-utilizing strain 34. Emended description of Methylovorus glucosotrophus is provided.
[Mh] Termos MeSH primário: DNA Bacteriano/genética
Frutose/metabolismo
Metanol/metabolismo
Methylophilaceae/classificação
Filogenia
RNA Ribossômico 16S/genética
[Mh] Termos MeSH secundário: Técnicas de Tipagem Bacteriana
Meios de Cultura/química
Meios de Cultura/farmacologia
Ácidos Graxos/química
Ácidos Graxos/isolamento & purificação
Frutose/farmacologia
Redes e Vias Metabólicas/genética
Metanol/farmacologia
Methylophilaceae/efeitos dos fármacos
Methylophilaceae/genética
Methylophilaceae/metabolismo
Fosfolipídeos/química
Fosfolipídeos/isolamento & purificação
Federação Russa
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Culture Media); 0 (DNA, Bacterial); 0 (Fatty Acids); 0 (Phospholipids); 0 (RNA, Ribosomal, 16S); 30237-26-4 (Fructose); Y4S76JWI15 (Methanol)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180208
[Lr] Data última revisão:
180208
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180125
[St] Status:MEDLINE


  7 / 14329 MEDLINE  
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[PMID]:28463494
[Au] Autor:Rohlhill J; Sandoval NR; Papoutsakis ET
[Ad] Endereço:Department of Chemical & Biomolecular Engineering and the Delaware Biotechnology Institute, University of Delaware , Newark, Delaware 19711, United States.
[Ti] Título:Sort-Seq Approach to Engineering a Formaldehyde-Inducible Promoter for Dynamically Regulated Escherichia coli Growth on Methanol.
[So] Source:ACS Synth Biol;6(8):1584-1595, 2017 Aug 18.
[Is] ISSN:2161-5063
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Tight and tunable control of gene expression is a highly desirable goal in synthetic biology for constructing predictable gene circuits and achieving preferred phenotypes. Elucidating the sequence-function relationship of promoters is crucial for manipulating gene expression at the transcriptional level, particularly for inducible systems dependent on transcriptional regulators. Sort-seq methods employing fluorescence-activated cell sorting (FACS) and high-throughput sequencing allow for the quantitative analysis of sequence-function relationships in a robust and rapid way. Here we utilized a massively parallel sort-seq approach to analyze the formaldehyde-inducible Escherichia coli promoter (P ) with single-nucleotide resolution. A library of mutated formaldehyde-inducible promoters was cloned upstream of gfp on a plasmid. The library was partitioned into bins via FACS on the basis of green fluorescent protein (GFP) expression level, and mutated promoters falling into each expression bin were identified with high-throughput sequencing. The resulting analysis identified two 19 base pair repressor binding sites, one upstream of the -35 RNA polymerase (RNAP) binding site and one overlapping with the -10 site, and assessed the relative importance of each position and base therein. Key mutations were identified for tuning expression levels and were used to engineer formaldehyde-inducible promoters with predictable activities. Engineered variants demonstrated up to 14-fold lower basal expression, 13-fold higher induced expression, and a 3.6-fold stronger response as indicated by relative dynamic range. Finally, an engineered formaldehyde-inducible promoter was employed to drive the expression of heterologous methanol assimilation genes and achieved increased biomass levels on methanol, a non-native substrate of E. coli.
[Mh] Termos MeSH primário: Proliferação Celular/genética
Proteínas de Escherichia coli/genética
Escherichia coli/fisiologia
Regulação Bacteriana da Expressão Gênica/genética
Engenharia Metabólica/métodos
Metanol/metabolismo
Regiões Promotoras Genéticas/genética
[Mh] Termos MeSH secundário: Algoritmos
Escherichia coli/citologia
Escherichia coli/efeitos dos fármacos
Citometria de Fluxo/métodos
Formaldeído/administração & dosagem
Regulação Bacteriana da Expressão Gênica/efeitos dos fármacos
Sequenciamento de Nucleotídeos em Larga Escala/métodos
Ativação Transcricional/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Escherichia coli Proteins); 1HG84L3525 (Formaldehyde); Y4S76JWI15 (Methanol)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180201
[Lr] Data última revisão:
180201
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170503
[St] Status:MEDLINE
[do] DOI:10.1021/acssynbio.7b00114


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[PMID]:27771305
[Au] Autor:Walter ME; Ortiz A; Sondgeroth C; Sindt NM; Duszenko N; Catlett JL; Zhou Y; Valloppilly S; Anderson C; Fernando S; Buan NR
[Ad] Endereço:Redox Biology Center, Department of Biochemistry, University of Nebraska-Lincoln, N200 Beadle Center, Lincoln, NE 68588-0664, United States.
[Ti] Título:High-throughput mutation, selection, and phenotype screening of mutant methanogenic archaea.
[So] Source:J Microbiol Methods;131:113-121, 2016 12.
[Is] ISSN:1872-8359
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Bacterial and archaeal genomes can contain 30% or more hypothetical genes with no predicted function. Phylogenetically deep-branching microbes, such as methane-producing archaea (methanogens), contain up to 50% genes with unknown function. In order to formulate hypotheses about the function of hypothetical gene functions in the strict anaerobe, Methanosarcina acetivorans, we have developed high-throughput anaerobic techniques to UV mutagenize, screen, and select for mutant strains in 96-well plates. Using these approaches we have isolated 10 mutant strains that exhibit a variety of physiological changes including increased or decreased growth rate relative to the parent strain when cells use methanol and/or acetate as carbon and energy sources. This method provides an avenue for the first step in identifying new gene functions: associating a genetic mutation with a reproducible phenotype. Mutations in bona fide methanogenesis genes such as corrinoid methyltransferases and proton-translocating F H :methanophenazine oxidoreductase (Fpo) were also generated, opening the door to in vivo functional complementation experiments. Irradiation-based mutagenesis such as from ultraviolet (UV) light, combined with modern genome sequencing, is a useful procedure to discern systems-level gene function in prokaryote taxa that can be axenically cultured but which may be resistant to chemical mutagens.
[Mh] Termos MeSH primário: Archaea/genética
Archaea/isolamento & purificação
Archaea/efeitos da radiação
Ensaios de Triagem em Larga Escala/métodos
Fenótipo
Mutação Puntual/efeitos da radiação
Raios Ultravioleta
[Mh] Termos MeSH secundário: Acetatos/metabolismo
Archaea/metabolismo
DNA Arqueal/genética
DNA Arqueal/efeitos da radiação
Genes Arqueais
Metano/metabolismo
Metanol/metabolismo
Methanosarcina/genética
Methanosarcina/crescimento & desenvolvimento
Methanosarcina/efeitos da radiação
Metiltransferases/genética
Viabilidade Microbiana/efeitos da radiação
Mutagênese/efeitos da radiação
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Acetates); 0 (DNA, Archaeal); EC 2.1.1.- (Methyltransferases); OP0UW79H66 (Methane); Y4S76JWI15 (Methanol)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:180126
[Lr] Data última revisão:
180126
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161107
[St] Status:MEDLINE


  9 / 14329 MEDLINE  
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[PMID]:27778473
[Au] Autor:Gihaz S; Weiser D; Dror A; Sátorhelyi P; Jerabek-Willemsen M; Poppe L; Fishman A
[Ad] Endereço:Department of Biotechnology and Food Engineering, Technion-Israel Institute of Technology, Haifa, 3200003, Israel.
[Ti] Título:Creating an Efficient Methanol-Stable Biocatalyst by Protein and Immobilization Engineering Steps towards Efficient Biosynthesis of Biodiesel.
[So] Source:ChemSusChem;9(22):3161-3170, 2016 11 23.
[Is] ISSN:1864-564X
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Two ternary sol-gel matrices, an octyltriethoxysilane-based aliphatic matrix and a phenyltriethoxysilane (PTEOS)-based aromatic matrix, were used to immobilize a methanol-stable variant of lipase from Geobacillus stearothermophilus T6 for the synthesis of biodiesel from waste oil. Superior thermal stability of the mutant versus the wildtype in methanol was confirmed by intrinsic protein fluorescence measurements. The influence of skim milk and soluble E. coli lysate proteins as bulking and stabilizing agents in conjunction with sol-gel entrapment were investigated. E. coli lysate proteins were better stabilizing agents of the purified lipase mutant than skim milk, as evidenced by reverse engineering of the aromatic-based system. This was also shown for commercial Candida antarctica lipase B (CaLB) and Thermomyces lanuginosus lipase (TLL). Uniform, dense, and nonaggregated particles imaged by scanning electron microscopy and a small particle size of 13 µm pertaining to the system comprising PTEOS and E. coli lysate proteins correlated well with high esterification activity. Combining protein and immobilization engineering resulted in a durable biocatalyst with efficient recycling ability and high biodiesel conversion rates.
[Mh] Termos MeSH primário: Biocatálise
Biocombustíveis
Enzimas Imobilizadas/química
Enzimas Imobilizadas/metabolismo
Lipase/química
Lipase/metabolismo
Metanol/farmacologia
[Mh] Termos MeSH secundário: Animais
Estabilidade Enzimática/efeitos dos fármacos
Enzimas Imobilizadas/genética
Proteínas de Escherichia coli/química
Geobacillus stearothermophilus/enzimologia
Hidrólise
Lipase/genética
Leite/química
Modelos Moleculares
Conformação Proteica
Engenharia de Proteínas
Temperatura Ambiente
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Biofuels); 0 (Enzymes, Immobilized); 0 (Escherichia coli Proteins); EC 3.1.1.3 (Lipase); Y4S76JWI15 (Methanol)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:180117
[Lr] Data última revisão:
180117
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161026
[St] Status:MEDLINE
[do] DOI:10.1002/cssc.201601158


  10 / 14329 MEDLINE  
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[PMID]:29185908
[Au] Autor:Prabhu AA; Venkata Dasu V
[Ad] Endereço:a Department of Biosciences and Bioengineering , Biochemical Engineering Laboratory, Indian Institute of Technology Guwahati , Guwahati , Assam , India.
[Ti] Título:Dual-substrate inhibition kinetic studies for recombinant human interferon gamma producing Pichia pastoris.
[So] Source:Prep Biochem Biotechnol;47(10):953-962, 2017 Nov 26.
[Is] ISSN:1532-2297
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Pichia pastoris is considered as one of the prominent host extensively used as a platform for heterologous protein production. In the present study, the growth inhibition kinetics of recombinant P. pastoris expressing human interferon gamma was studied under different initial substrate concentrations of gluconate (10-100 g L ) and methanol (2-50 g L ) in modified FM22 medium. The highest specific growth rate of 0.0206 and 0.019 hr was observed at 60 g L of gluconate and 10 g L of methanol, respectively. Various three- and four-parametric Monod-variant models were chosen to analyze the inhibition kinetics. The model parameters as well as goodness of fit were estimated using nonlinear regression analysis. The three-parameter Haldane model was found to be best fit for both gluconate (R = 0.95) and methanol substrate (R = 0.96). The parameter sensitivity analysis revealed that µ , K , and K are the most sensitive parameters for both methanol and gluconate. Different substrate inhibition models were fitted to the growth kinetic data and the additive form of double Webb model was found to be the best to explain the growth kinetics of recombinant P. pastoris.
[Mh] Termos MeSH primário: Gluconatos/metabolismo
Microbiologia Industrial/métodos
Interferon gama/metabolismo
Metanol/metabolismo
Pichia/crescimento & desenvolvimento
[Mh] Termos MeSH secundário: Meios de Cultura/metabolismo
Seres Humanos
Cinética
Pichia/metabolismo
Proteínas Recombinantes/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Culture Media); 0 (Gluconates); 0 (IFNG protein, human); 0 (Recombinant Proteins); 82115-62-6 (Interferon-gamma); R4R8J0Q44B (gluconic acid); Y4S76JWI15 (Methanol)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171225
[Lr] Data última revisão:
171225
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171130
[St] Status:MEDLINE
[do] DOI:10.1080/10826068.2017.1350977



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