Base de dados : MEDLINE
Pesquisa : D02.033.800.400 [Categoria DeCS]
Referências encontradas : 369 [refinar]
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[PMID]:27956522
[Au] Autor:Nolle N; Felsl A; Heermann R; Fuchs TM
[Ad] Endereço:Lehrstuhl für Mikrobielle Ökologie, ZIEL-Institute for Food and Health, Wissenschaftszentrum Weihenstephan, Technische Universität München, Freising, Germany.
[Ti] Título:Genetic Characterization of the Galactitol Utilization Pathway of Salmonella enterica Serovar Typhimurium.
[So] Source:J Bacteriol;199(4), 2017 Feb 15.
[Is] ISSN:1098-5530
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Galactitol degradation by salmonellae remains underinvestigated, although this metabolic capability contributes to growth in animals (R. R. Chaudhuri et al., PLoS Genet 9:e1003456, 2013, https://doi.org/10.1371/journal.pgen.1003456). The genes responsible for this metabolic capability are part of a 9.6-kb gene cluster that spans from gatY to gatR (STM3253 to STM3262) and encodes a phosphotransferase system, four enzymes, and a transporter of the major facilitator superfamily. Genome comparison revealed the presence of this genetic determinant in nearly all Salmonella strains. The generation time of Salmonella enterica serovar Typhimurium strain ST4/74 was higher in minimal medium with galactitol than with glucose. Knockout of STM3254 and gatC resulted in a growth-deficient phenotype of S Typhimurium, with galactitol as the sole carbon source. Partial deletion of gatR strongly reduced the lag phase of growth with galactitol, whereas strains overproducing GatR exhibited a near-zero growth phenotype. Luciferase reporter assays demonstrated strong induction of the gatY and gatZ promoters, which control all genes of this cluster except gatR, in the presence of galactitol but not glucose. Purified GatR bound to these two main gat gene cluster promoters as well as to its own promoter, demonstrating that this autoregulated repressor controls galactitol degradation. Surface plasmon resonance spectroscopy revealed distinct binding properties of GatR toward the three promoters, resulting in a model of differential gat gene expression. The cyclic AMP receptor protein (CRP) bound these promoters with similarly high affinities, and a mutant lacking crp showed severe growth attenuation, demonstrating that galactitol utilization is subject to catabolite repression. Here, we provide the first genetic characterization of galactitol degradation in Salmonella, revealing novel insights into the regulation of this dissimilatory pathway. IMPORTANCE: The knowledge of how pathogens adapt their metabolism to the compartments encountered in hosts is pivotal to our understanding of bacterial infections. Recent research revealed that enteropathogens have adapted specific metabolic pathways that contribute to their virulence properties, for example, by helping to overcome limitations in nutrient availability in the gut due to colonization resistance. The capability of Salmonella enterica serovar Typhimurium to degrade galactitol has already been demonstrated to play a role in vivo, but it has not been investigated so far on the genetic level. To our knowledge, this is the first molecular description of the galactitol degradation pathway of a pathogen.
[Mh] Termos MeSH primário: Proteínas de Bactérias/metabolismo
Galactitol/metabolismo
Regulação Bacteriana da Expressão Gênica/fisiologia
Salmonella typhimurium/metabolismo
[Mh] Termos MeSH secundário: Proteínas de Bactérias/genética
Clonagem Molecular
DNA Bacteriano/genética
Família Multigênica
Regiões Promotoras Genéticas
Ligação Proteica
Salmonella typhimurium/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (DNA, Bacterial); 113ZQ1Y7DD (Galactitol)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170815
[Lr] Data última revisão:
170815
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161214
[St] Status:MEDLINE


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[PMID]:27736666
[Au] Autor:Righi G; Mandic' E; Sappino C; Dema E; Bovicelli P
[Ad] Endereço:CNR-IBPM, "Sapienza" University of Rome, Dep. Chemistry, P.le A. Moro 5, 00185, Rome, Italy. Electronic address: giuliana.righi@cnr.it.
[Ti] Título:Asymmetric routes toward polyhydroxylated pyrrolidines: Synthesis of 1,4-dideoxy-1,4-imino-d-galactitol and 1,4-dideoxy-1,4-imino-d-glucitol.
[So] Source:Carbohydr Res;435:100-105, 2016 Nov 29.
[Is] ISSN:1873-426X
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Herein the total synthesis of the pyrrolidine alkaloids 1,4-dideoxy-1,4-imino-d-galactitol and its diastereoisomer 1,4-dideoxy-1,4-imino-d-glucitol is described, starting from a common optically active precursor. The key step in our approach was the double diastereoselection in the asymmetric dihydroxylation of chiral vinyl azido alcohols, obtained by means of two different regio- and stereoselective nucleophilic openings of the corresponding chiral vinyl epoxide.
[Mh] Termos MeSH primário: Galactitol/química
Pirrolidinas/síntese química
Sorbitol/química
[Mh] Termos MeSH secundário: Estrutura Molecular
Pirrolidinas/química
Estereoisomerismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Pyrrolidines); 113ZQ1Y7DD (Galactitol); 506T60A25R (Sorbitol)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170227
[Lr] Data última revisão:
170227
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161014
[St] Status:MEDLINE


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[PMID]:26893047
[Au] Autor:Natarajan J; Madras G; Chatterjee K
[Ad] Endereço:Centre for Nano Science and Engineering, Indian Institute of Science, Bangalore 560012, India.
[Ti] Título:Tailoring the degradation rate and release kinetics from poly(galactitol sebacate) by blending with chitosan, alginate or ethyl cellulose.
[So] Source:Int J Biol Macromol;93(Pt B):1591-1602, 2016 Dec.
[Is] ISSN:1879-0003
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Despite significant advances in recent times, the investigation of discovering a perfect biomaterial is perennial. In this backdrop, blending of natural and synthetic polymers is gaining popularity since it is the easiest way to complement the drawbacks and attain a superlative material. Based on this, the objective of this study was to synthesize a novel polyester, poly(galactitol sebacate), and subsequently blend this polymer with one of the three natural polymers such as alginate, chitosan or ethyl cellulose. FT-IR showed the presence of both the polymers in the blends. H NMR confirmed the chemical structure of the synthesized poly (galactitol sebacate). Thermal characterization was performed by DSC revealing that the polymers were amorphous in nature and the glass transition temperatures increased with the increase in ratio of the natural polymers in the blends. SEM imaging showed that the blends were predominantly homogeneous. Contact angle measurements demonstrated that the blending imparted the hydrophilic nature into poly (galactitol sebacate) when blending with alginate or chitosan and hydrophobic when blending with ethyl cellulose. In vitro hydrolytic degradation studies and dye release studies indicated that the polymers became more hydrophilic in alginate and chitosan blends and thus accelerated the degradation and release process. The reverse trend was observed in the case of ethyl cellulose blends. Modeling elucidated that the degradation and dye release followed first order kinetics and Higuchi kinetics, respectively. In vitro cell studies confirmed the cytocompatible nature of the blends. It can be proposed that the chosen natural polymers for blending showed wide variations in hydrophilicity resulting in tailored degradation, release and cytocompatibility properties and thus are promising candidates for use in drug delivery and tissue engineering.
[Mh] Termos MeSH primário: Alginatos/química
Materiais Biocompatíveis/química
Celulose/análogos & derivados
Quitosana/química
[Mh] Termos MeSH secundário: Animais
Materiais Biocompatíveis/toxicidade
Linhagem Celular
Sobrevivência Celular/efeitos dos fármacos
Celulose/química
Portadores de Fármacos
Liberação Controlada de Fármacos
Galactitol/química
Hidrólise
Interações Hidrofóbicas e Hidrofílicas
Cinética
Teste de Materiais
Camundongos
Poliésteres/química
Engenharia Tecidual
Tecidos Suporte/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Alginates); 0 (Biocompatible Materials); 0 (Drug Carriers); 0 (Polyesters); 113ZQ1Y7DD (Galactitol); 7Z8S9VYZ4B (ethyl cellulose); 9004-34-6 (Cellulose); 9012-76-4 (Chitosan)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170407
[Lr] Data última revisão:
170407
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160220
[St] Status:MEDLINE


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[PMID]:26783274
[Au] Autor:Martinelli D; Bernardi B; Napolitano A; Colafati GS; Dionisi-Vici C
[Ad] Endereço:From the Division of Metabolism, Department of Paediatric Medicine (D.M., C.D.-V.), Neuroradiology Unit (B.B., G.S.C.), and Unit of Imaging Research (A.N.), Bambino Gesù Children's Hospital, IRCCS, Rome, Italy. diego.martinelli@opbg.net.
[Ti] Título:Teaching NeuroImages: Galactitol peak and fatal cerebral edema in classic galactosemia: Too much sugar in the brain.
[So] Source:Neurology;86(3):e32-3, 2016 Jan 19.
[Is] ISSN:1526-632X
[Cp] País de publicação:United States
[La] Idioma:eng
[Mh] Termos MeSH primário: Edema Encefálico/diagnóstico
Galactitol/metabolismo
Galactosemias/diagnóstico
[Mh] Termos MeSH secundário: Edema Encefálico/metabolismo
Evolução Fatal
Feminino
Galactosemias/genética
Galactosemias/metabolismo
Seres Humanos
Recém-Nascido
Imagem por Ressonância Magnética
Neuroimagem/métodos
Neurologia/educação
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
113ZQ1Y7DD (Galactitol)
[Em] Mês de entrada:1605
[Cu] Atualização por classe:160119
[Lr] Data última revisão:
160119
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:160120
[St] Status:MEDLINE
[do] DOI:10.1212/WNL.0000000000002284


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[PMID]:26474980
[Au] Autor:Kim BC; Seung Jeon B; Kim S; Kim H; Um Y; Sang BI
[Ad] Endereço:1​The Research Institute of Industrial Science, Hanyang University, 17 Hangdang-dong, Seongdong-gu, Seoul 133-791, Republic of Korea.
[Ti] Título:Caproiciproducens galactitolivorans gen. nov., sp. nov., a bacterium capable of producing caproic acid from galactitol, isolated from a wastewater treatment plant.
[So] Source:Int J Syst Evol Microbiol;65(12):4902-8, 2015 Dec.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A strictly anaerobic, Gram-stain-positive, non-spore-forming, rod-shaped bacterial strain, designated BS-1T, was isolated from an anaerobic digestion reactor during a study of bacteria utilizing galactitol as the carbon source. Its cells were 0.3-0.5 µm × 2-4 µm, and they grew at 35-45 °C and at pH 6.0-8.0. Strain BS-1T produced H2, CO2, ethanol, acetic acid, butyric acid and caproic acid as metabolic end products of anaerobic fermentation. Phylogenetic analysis, based on the 16S rRNA gene sequence, showed that strain BS-1T represented a novel bacterial genus within the family Ruminococcaceae, Clostridium Cluster IV. The type strains that were most closely related to strain BS-1T were Clostridium sporosphaeroides KCTC 5598T (94.5 %), Clostridium leptum KCTC 5155T (94.3 %), Ruminococcus bromii ATCC 27255T (92.1 %) and Ethanoligenens harbinense YUAN-3T (91.9 %). Strain BS-1T had 17.6 % and 20.9 % DNA-DNA relatedness values with C. sporosphaeroides DSM 1294T and C. leptum DSM 753T, respectively. The major components of the cellular fatty acids were C16 : 0 dimethyl aldehyde (DMA) (22.1 %), C16 : 0 aldehyde (14.1 %) and summed feature 11 (iso-C17 : 0 3-OH and/or C18 : 2 DMA; 10.0 %). The genomic DNA G+C content was 50.0 mol%. Phenotypic and phylogenetic characteristics allowed strain BS-1T to be clearly distinguished from other taxa of the genus Clostridium Cluster IV. On the basis of these data, the isolate is considered to represent a novel genus and novel species within Clostridium Cluster IV, for which the name Caproiciproducens galactitolivorans gen. nov., sp. nov. is proposed. The type species is BS-1T ( = JCM 30532T and KCCM 43048T).
[Mh] Termos MeSH primário: Caproatos/metabolismo
Clostridiales/classificação
Galactitol/metabolismo
Filogenia
Águas Residuais/microbiologia
[Mh] Termos MeSH secundário: Técnicas de Tipagem Bacteriana
Composição de Bases
Clostridiales/genética
Clostridiales/isolamento & purificação
DNA Bacteriano/genética
Ácidos Graxos/química
Dados de Sequência Molecular
Hibridização de Ácido Nucleico
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Caproates); 0 (DNA, Bacterial); 0 (Fatty Acids); 0 (RNA, Ribosomal, 16S); 0 (Waste Water); 113ZQ1Y7DD (Galactitol); 1F8SN134MX (hexanoic acid)
[Em] Mês de entrada:1606
[Cu] Atualização por classe:160120
[Lr] Data última revisão:
160120
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151018
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.000665


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[PMID]:26472925
[Au] Autor:Wichelecki DJ; Vetting MW; Chou L; Al-Obaidi N; Bouvier JT; Almo SC; Gerlt JA
[Ad] Endereço:From the Departments of Biochemistry and Chemistry and Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801 and.
[Ti] Título:ATP-binding Cassette (ABC) Transport System Solute-binding Protein-guided Identification of Novel d-Altritol and Galactitol Catabolic Pathways in Agrobacterium tumefaciens C58.
[So] Source:J Biol Chem;290(48):28963-76, 2015 Nov 27.
[Is] ISSN:1083-351X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Innovations in the discovery of the functions of uncharacterized proteins/enzymes have become increasingly important as advances in sequencing technology flood protein databases with an exponentially growing number of open reading frames. This study documents one such innovation developed by the Enzyme Function Initiative (EFI; U54GM093342), the use of solute-binding proteins for transport systems to identify novel metabolic pathways. In a previous study, this strategy was applied to the tripartite ATP-independent periplasmic transporters. Here, we apply this strategy to the ATP-binding cassette transporters and report the discovery of novel catabolic pathways for d-altritol and galactitol in Agrobacterium tumefaciens C58. These efforts resulted in the description of three novel enzymatic reactions as follows: 1) oxidation of d-altritol to d-tagatose via a dehydrogenase in Pfam family PF00107, a previously unknown reaction; 2) phosphorylation of d-tagatose to d-tagatose 6-phosphate via a kinase in Pfam family PF00294, a previously orphan EC number; and 3) epimerization of d-tagatose 6-phosphate C-4 to d-fructose 6-phosphate via a member of Pfam family PF08013, another previously unknown reaction. The epimerization reaction catalyzed by a member of PF08013 is especially noteworthy, because the functions of members of PF08013 have been unknown. These discoveries were assisted by the following two synergistic bioinformatics web tools made available by the Enzyme Function Initiative: the EFI-Enzyme Similarity Tool and the EFI-Genome Neighborhood Tool.
[Mh] Termos MeSH primário: Transportadores de Cassetes de Ligação de ATP/metabolismo
Agrobacterium tumefaciens/metabolismo
Proteínas de Bactérias/metabolismo
Galactitol/metabolismo
Álcoois Açúcares/metabolismo
[Mh] Termos MeSH secundário: Transportadores de Cassetes de Ligação de ATP/genética
Agrobacterium tumefaciens/genética
Proteínas de Bactérias/genética
Galactitol/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Sugar Alcohols); 113ZQ1Y7DD (Galactitol); 5552-13-6 (altritol)
[Em] Mês de entrada:1603
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151017
[St] Status:MEDLINE
[do] DOI:10.1074/jbc.M115.686857


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[PMID]:25453932
[Au] Autor:Tathod AP; Dhepe PL
[Ad] Endereço:Catalysis and Inorganic Chemistry Division, CSIR-National Chemical Laboratory, Dr. Homi Bhabha Road, Pune 411008, India.
[Ti] Título:Efficient method for the conversion of agricultural waste into sugar alcohols over supported bimetallic catalysts.
[So] Source:Bioresour Technol;178:36-44, 2015 Feb.
[Is] ISSN:1873-2976
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Promoter effect of Sn in the PtSn/γ-Al2O3 (AL) and PtSn/C bimetallic catalysts is studied for the conversion of variety of substrates such as, C5 sugars (xylose, arabinose), C6 sugars (glucose, fructose, galactose), hemicelluloses (xylan, arabinogalactan), inulin and agricultural wastes (bagasse, rice husk, wheat straw) into sugar alcohols (sorbitol, mannitol, xylitol, arabitol, galactitol). In all the reactions, PtSn/AL showed enhanced yields of sugar alcohols by 1.5-3 times than Pt/AL. Compared to C, AL supported bimetallic catalysts showed prominent enhancement in the yields of sugar alcohols. Bimetallic catalysts characterized by X-ray diffraction study revealed the stability of catalyst and absence of alloy formation thereby indicating that Pt and Sn are present as individual particles in PtSn/AL. The TEM analysis also confirmed stability of the catalysts and XPS study disclosed formation of electron deficient Sn species which helps in polarizing carbonyl bond to achieve enhanced hydrogenation activity.
[Mh] Termos MeSH primário: Álcoois/química
Biotecnologia/métodos
Resíduos
[Mh] Termos MeSH secundário: Agricultura
Arabinose/química
Reatores Biológicos
Carbono/química
Catálise
Frutose/química
Galactanos/química
Galactitol/química
Galactose/química
Glucose/química
Hidrogênio/química
Inulina/química
Manitol/química
Microscopia Eletrônica de Transmissão
Platina/química
Sorbitol/química
Álcoois Açúcares/química
Temperatura Ambiente
Estanho/química
Triticum
Difração de Raios X
Xilanos/química
Xilitol/química
Xilose/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Alcohols); 0 (Galactans); 0 (Sugar Alcohols); 0 (Waste Products); 0 (Xylans); 113ZQ1Y7DD (Galactitol); 30237-26-4 (Fructose); 3OWL53L36A (Mannitol); 49DFR088MY (Platinum); 506T60A25R (Sorbitol); 7440-31-5 (Tin); 7440-44-0 (Carbon); 7YNJ3PO35Z (Hydrogen); 9005-80-5 (Inulin); A1TA934AKO (Xylose); B40ROO395Z (Arabinose); IY9XDZ35W2 (Glucose); SL4SX1O487 (arabinogalactan); VCQ006KQ1E (Xylitol); X2RN3Q8DNE (Galactose); YFV05Y57M9 (arabitol)
[Em] Mês de entrada:1509
[Cu] Atualização por classe:161125
[Lr] Data última revisão:
161125
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:141203
[St] Status:MEDLINE


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[PMID]:24731824
[Au] Autor:Jagtap SS; Singh R; Kang YC; Zhao H; Lee JK
[Ad] Endereço:Department of Chemical Engineering, Konkuk University, Seoul 143-701, Republic of Korea.
[Ti] Título:Cloning and characterization of a galactitol 2-dehydrogenase from Rhizobium legumenosarum and its application in D-tagatose production.
[So] Source:Enzyme Microb Technol;58-59:44-51, 2014 May 10.
[Is] ISSN:1879-0909
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Galactitol 2-dehydrogenase (GDH) belongs to the protein subfamily of short-chain dehydrogenases/reductases and can be used to produce optically pure building blocks and for the bioconversion of bioactive compounds. An NAD(+)-dependent GDH from Rhizobium leguminosarum bv. viciae 3841 (RlGDH) was cloned and overexpressed in Escherichia coli. The RlGDH protein was purified as an active soluble form using His-tag affinity chromatography. The molecular mass of the purified enzyme was estimated to be 28kDa by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and 114kDa by gel filtration chromatography, suggesting that the enzyme is a homotetramer. The enzyme has an optimal pH and temperature of 9.5 and 35°C, respectively. The purified recombinant RlGDH catalyzed the oxidation of a wide range of substrates, including polyvalent aliphatic alcohols and polyols, to the corresponding ketones and ketoses. Among various polyols, galactitol was the preferred substrate of RlGDH with a Km of 8.8mM, kcat of 835min(-1) and a kcat/Km of 94.9min(-1)mM(-1). Although GDHs have been characterized from a few other sources, RlGDH is distinguished from other GDHs by its higher specific activity for galactitol and broad substrate spectrum, making RlGDH a good choice for practical applications.
[Mh] Termos MeSH primário: Proteínas de Bactérias/isolamento & purificação
Rhizobium leguminosarum/enzimologia
Desidrogenase do Álcool de Açúcar/isolamento & purificação
[Mh] Termos MeSH secundário: Motivos de Aminoácidos
Sequência de Aminoácidos
Proteínas de Bactérias/química
Proteínas de Bactérias/genética
Proteínas de Bactérias/metabolismo
Catálise
Cromatografia de Afinidade
Cromatografia em Gel
Clonagem Molecular
Eletroforese em Gel de Poliacrilamida
Escherichia coli/metabolismo
Galactitol/metabolismo
Genes Bacterianos
Concentração de Íons de Hidrogênio
Modelos Moleculares
Dados de Sequência Molecular
Peso Molecular
Conformação Proteica
Proteínas Recombinantes de Fusão/metabolismo
Rhizobium leguminosarum/genética
Alinhamento de Sequência
Homologia de Sequência de Aminoácidos
Especificidade por Substrato
Desidrogenase do Álcool de Açúcar/química
Desidrogenase do Álcool de Açúcar/genética
Desidrogenase do Álcool de Açúcar/metabolismo
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Recombinant Fusion Proteins); 113ZQ1Y7DD (Galactitol); EC 1.1.- (Sugar Alcohol Dehydrogenases); EC 1.1.1.16 (galactitol 2-dehydrogenase)
[Em] Mês de entrada:1412
[Cu] Atualização por classe:140415
[Lr] Data última revisão:
140415
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140416
[St] Status:MEDLINE


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[PMID]:24337250
[Au] Autor:Nduko JM; Matsumoto K; Ooi T; Taguchi S
[Ad] Endereço:Division of Biotechnology and Macromolecular Chemistry, Graduate School of Engineering, Hokkaido University, N13-W8, Kita-ku, Sapporo, 060-8628, Japan.
[Ti] Título:Enhanced production of poly(lactate-co-3-hydroxybutyrate) from xylose in engineered Escherichia coli overexpressing a galactitol transporter.
[So] Source:Appl Microbiol Biotechnol;98(6):2453-60, 2014 Mar.
[Is] ISSN:1432-0614
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Poly(lactate-co-3-hydroxybutyrate) (P(LA-co-3HB)) was previously produced from xylose in engineered Escherichia coli. The aim of this study was to increase the polymer productivity and LA fraction in P(LA-co-3HB) using two metabolic engineering approaches: (1) deletions of competing pathways to lactate production and (2) overexpression of a galactitol transporter (GatC), which contributes to the ATP-independent xylose uptake. Engineered E. coli mutants (ΔpflA, Δpta, ΔackA, ΔpoxB, Δdld, and a dual mutant; ΔpflA + Δdld) and their parent strain, BW25113, were grown on 20 g l(-1) xylose for P(LA-co-3HB) production. The single deletions of ΔpflA, Δpta, and Δdld increased the LA fraction (58-66 mol%) compared to BW25113 (56 mol%). In particular, the ΔpflA + Δdld strain produced P(LA-co-3HB) containing 73 mol% LA. Furthermore, GatC overexpression increased both polymer yields and LA fractions in ΔpflA, Δpta, and Δdld mutants, and BW25113. The ΔpflA + gatC strain achieved a productivity of 8.3 g l(-1), which was 72 % of the theoretical maximum yield. Thus, to eliminate limitation of the carbon source, higher concentration of xylose was fed. As a result, BW25113 harboring gatC grown on 40 g l(-1) xylose reached the highest P(LA-co-3HB) productivity of 14.4 g l(-1). On the other hand, the ΔpflA + Δdld strain grown on 30 g l(-1) xylose synthesized 6.4 g l(-1) P(LA-co-3HB) while maintaining the highest LA fraction (73 mol%). The results indicated the usefulness of GatC for enhanced production of P(LA-co-3HB) from xylose, and the gene deletions to upregulate the LA fraction in P(LA-co-3HB). The polymers obtained had weight-averaged molecular weights in the range of 34,000-114,000.
[Mh] Termos MeSH primário: Escherichia coli/enzimologia
Escherichia coli/metabolismo
Galactitol/metabolismo
Proteínas de Membrana Transportadoras/metabolismo
Engenharia Metabólica
Poliésteres/metabolismo
Xilose/metabolismo
[Mh] Termos MeSH secundário: Escherichia coli/genética
Deleção de Genes
Expressão Gênica
Proteínas de Membrana Transportadoras/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Membrane Transport Proteins); 0 (Polyesters); 113ZQ1Y7DD (Galactitol); A1TA934AKO (Xylose)
[Em] Mês de entrada:1410
[Cu] Atualização por classe:140227
[Lr] Data última revisão:
140227
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:131217
[St] Status:MEDLINE
[do] DOI:10.1007/s00253-013-5401-0


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[PMID]:24256145
[Au] Autor:Zhang P; Zhang Z; Kador PF
[Ad] Endereço:1 Department of Pharmaceutical Sciences, College of Pharmacy, University of Nebraska Medical Center , Omaha, Nebraska.
[Ti] Título:Polyol effects on growth factors and MAPK signaling in rat retinal capillary cells.
[So] Source:J Ocul Pharmacol Ther;30(1):4-11, 2014 Feb.
[Is] ISSN:1557-7732
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:PURPOSE: Recent studies report that growth factor and signaling changes in rat lenses do not directly result from the presence of diabetes or sorbitol/galactitol (polyol) formation/accumulation, but from secondary osmotic changes associated with the aldose reductase (AR) catalyzed polyol formation. AR is also present in rat retinal pericyte and endothelial cells; however, significant polyol formation only occurs in pericytes and this does not appear to be linked to osmotic changes. The purpose of this study was to determine whether polyol formation and AR activity are similarly linked to growth factor and signaling changes in the rat capillary cells despite the apparent absence of osmotic stress. METHODS: Conditionally immortalized rat retinal pericyte (TR-rPCT) and endothelial (TR-iBRB) cell lines were cultured on collagen type 1-coated dishes in the DMEM containing 5.5 mM glucose. After 24 h of initial culture, the medium was replaced with a serum-free medium containing 5.5, 25, or 50 mM glucose or galactose with/without the aldose reductase inhibitors (ARIs) AL1576 or tolrestat for periods of up to 48 h. Growth factors and transduction pathways were measured by Western blots using the antibodies against basic FGF, IGF-1, TGF-ß, P-ERK1/2, P-SAPK/JNK, and P-Akt. RESULTS: Sorbitol accumulation was only observed in pericytes, while galactitol was present in both pericytes and endothelial cells. Pericytes cultured in high glucose showed increased expression of the growth factors basic FGF, IGF-1, TGF-ß, and signaling in P-Akt, P-ERK1/2, and P-SAPK/JNK compared with those cultured in 5.5 mM glucose and these expressions were normalized by the presence of ARIs. Similar results were observed with galactose media. In contrast, endothelial cells cultured in high glucose media showed neither growth factor or signaling changes. In galactose media, endothelial cells showed increased expression of basic FGF, IGF-1, TGF-ß, P-ERK1/2, and P-SAPK/JNK, which were only partially reduced by ARIs. CONCLUSION: Growth factor and MAPK signaling expression in pericytes are linked to the presence of polyols. Pericytes, which readily accumulate sorbitol/galactitol that is inhibited by ARIs, show expression changes similar to those observed in rat lenses. In contrast, endothelial cells only show partial expression changes that are linked to galactitol accumulation.
[Mh] Termos MeSH primário: Aldeído Redutase/metabolismo
Galactitol/metabolismo
Vasos Retinianos/metabolismo
Sorbitol/metabolismo
[Mh] Termos MeSH secundário: Aldeído Redutase/antagonistas & inibidores
Animais
Capilares/citologia
Capilares/metabolismo
Linhagem Celular
Células Endoteliais/metabolismo
Fluorenos/farmacologia
Galactose/química
Glucose/química
Hidantoínas/farmacologia
Peptídeos e Proteínas de Sinalização Intercelular/metabolismo
Sistema de Sinalização das MAP Quinases/fisiologia
Naftalenos/farmacologia
Pericitos/metabolismo
Ratos
Ratos Transgênicos
Vasos Retinianos/citologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL
[Nm] Nome de substância:
0 (Fluorenes); 0 (Hydantoins); 0 (Intercellular Signaling Peptides and Proteins); 0 (Naphthalenes); 0PM69S95UQ (imirestat); 0T93LG5NMK (tolrestat); 113ZQ1Y7DD (Galactitol); 506T60A25R (Sorbitol); EC 1.1.1.21 (Aldehyde Reductase); IY9XDZ35W2 (Glucose); X2RN3Q8DNE (Galactose)
[Em] Mês de entrada:1409
[Cu] Atualização por classe:161019
[Lr] Data última revisão:
161019
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:131122
[St] Status:MEDLINE
[do] DOI:10.1089/jop.2013.0170



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