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[PMID]:29194015
[Au] Autor:Tripathi P; A JL; Kapoor M
[Ad] Endereço:a Department of Protein Chemistry and Technology , CSIR-Central Food Technological Research Institute , Mysuru , Karnataka , India.
[Ti] Título:Phytase from Citrobacter koseri PM-7: Enhanced production using statistical method and application in ameliorating mineral bioaccessibility and protein digestibility of high-phytate food.
[So] Source:Prep Biochem Biotechnol;48(1):84-91, 2018 Jan 02.
[Is] ISSN:1532-2297
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The present study was aimed at enhancing phytase (Phy-Ck) production from Citrobacter koseri PM-7 using response surface methodology (RSM) and improving the bioaccessibility of minerals (Fe and Zn) and protein digestibility in high-phytate food using Phy-Ck. A five-variable and three-level central composite design of RSM using wheat bran (6.681%, w/v), inoculum level (2.5%, v/v), and triton X-100 (0.2%, v/v) resulted in up to 5.57-fold (1.047 U/ml) improvement in Phy-Ck yield from C. koseri PM-7 when compared with fermentation media I and II. The model was successfully validated in the design space by taking a random set of variable combinations. Treatment of high-phytate food with partially purified Phy-Ck showed improvement in mineral bioaccessibility maximally for defatted sesame flour (DSF) (Fe 45.5%; Zn 50.7%) followed by wheat flour (WF) (Fe 13.5%; Zn 14.4%), green gram flour (GGF) (Fe 0.7%; Zn 3.8%) and defatted groundnut flour (DGF) (Zn 5.6%). The in vitro protein digestibility (IVPD) of WF increased from 48.83 to 65.04%, GGF from 45.04 to 57.12%, and DSF from 47.34 to 55.7% after Phy-Ck treatment.
[Mh] Termos MeSH primário: 6-Fitase/metabolismo
Citrobacter koseri/enzimologia
[Mh] Termos MeSH secundário: Ração Animal/análise
Fibras na Dieta/análise
Fermentação
Farinha/análise
Ferro/metabolismo
Ácido Fítico/análise
Ácido Fítico/metabolismo
Proteólise
Triticum/química
Zinco/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Dietary Fiber); 7IGF0S7R8I (Phytic Acid); E1UOL152H7 (Iron); EC 3.1.3.26 (6-Phytase); J41CSQ7QDS (Zinc)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180308
[Lr] Data última revisão:
180308
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171202
[St] Status:MEDLINE
[do] DOI:10.1080/10826068.2017.1405024


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[PMID]:29282978
[Au] Autor:Li L; Fu Q; Xia M; Xin L; Shen H; Li G; Ji G; Meng Q; Xie Y
[Ad] Endereço:Research Center for Health and Nutrition, Shanghai University of Traditional Chinese Medicine , Shanghai 201203, China.
[Ti] Título:Inhibition of P-Glycoprotein Mediated Efflux in Caco-2 Cells by Phytic Acid.
[So] Source:J Agric Food Chem;66(4):988-998, 2018 Jan 31.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Phytic acid (IP6) is a natural phosphorylated inositol, which is abundantly present in most cereal grains and seeds. This study investigated the effects of IP6 regulation on P-glycoprotein (P-gp) and its potential mechanisms using in situ and in vitro models. The effective permeability of the typical P-gp substrate rhodamine 123 (R123) in colon was significantly increased from (1.69 ± 0.22) × 10 cm/s in the control group to (3.39 ± 0.417) × 10 cm/s (p < 0.01) in the 3.5 mM IP6 group. Additionally, IP6 can concentration-dependently decrease the R123 efflux ratio in both Caco-2 and MDCK II-MDR1 cell monolayers and increase intracellular R123 accumulation in Caco-2 cells. Furthermore, IP6 noncompetitively inhibited P-gp by impacting R123 efflux kinetics. The noncompetitive inhibition of P-gp by IP6 was likely due to decreases in P-gp ATPase activity and P-gp molecular conformational changes induced by IP6. In summary, IP6 is a promising P-gp inhibitor candidate.
[Mh] Termos MeSH primário: Membro 1 da Subfamília B de Cassetes de Ligação de ATP/antagonistas & inibidores
Ácido Fítico/farmacologia
[Mh] Termos MeSH secundário: Membro 1 da Subfamília B de Cassetes de Ligação de ATP/química
Membro 1 da Subfamília B de Cassetes de Ligação de ATP/genética
Adenosina Trifosfatases/metabolismo
Regulação Alostérica
Animais
Transporte Biológico/efeitos dos fármacos
Células CACO-2
Permeabilidade da Membrana Celular/efeitos dos fármacos
Colo/metabolismo
Cães
Seres Humanos
Rim/metabolismo
Células Madin Darby de Rim Canino
Conformação Molecular/efeitos dos fármacos
RNA Mensageiro/análise
Ratos
Ratos Sprague-Dawley
Rodamina 123/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (ATP-Binding Cassette, Sub-Family B, Member 1); 0 (RNA, Messenger); 1N3CZ14C5O (Rhodamine 123); 7IGF0S7R8I (Phytic Acid); EC 3.6.1.- (Adenosine Triphosphatases)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180212
[Lr] Data última revisão:
180212
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171229
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jafc.7b04307


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[PMID]:29199120
[Au] Autor:Jorge SE; Bringas M; Petruk AA; Arrar M; Marti MA; Skaf MS; Costa FF; Capece L; Sonati MF; Estrin D
[Ad] Endereço:Department of Clinical Pathology, School of Medical Sciences, University of Campinas (Unicamp), Campinas, SP, Brazil.
[Ti] Título:Understanding the molecular basis of the high oxygen affinity variant human hemoglobin Coimbra.
[So] Source:Arch Biochem Biophys;637:73-78, 2018 01 01.
[Is] ISSN:1096-0384
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Human hemoglobin (Hb) Coimbra (ßAsp99Glu) is one of the seven ßAsp99 Hb variants described to date. All ßAsp99 substitutions result in increased affinity for O and decreased heme-heme cooperativity and their carriers are clinically characterized by erythrocytocis, caused by tissue hypoxia. Since ßAsp99 plays an important role in the allosteric α1ß2 interface and the mutation in Hb Coimbra only represents the insertion of a CH group in this interface, the present study of Hb Coimbra is important for a better understanding of the global impact of small modifications in this allosteric interface. We carried out functional, kinetic and dynamic characterization of this hemoglobin, focusing on the interpretation of these results in the context of a growth of the position 99 side chain length in the α1ß2 interface. Oxygen affinity was evaluated by measuring p50 values in distinct pHs (Bohr effect), and the heme-heme cooperativity was analyzed by determining the Hill coefficient (n), in addition to the effect of the allosteric effectors inositol hexaphosphate (IHP) and 2,3-bisphosphoglyceric acid (2,3-BPG). Computer simulations revealed a stabilization of the R state in the Coimbra variant with respect to the wild type, and consistently, the T-to-R quaternary transition was observed on the nanosecond time scale of classical molecular dynamics simulations.
[Mh] Termos MeSH primário: Hemoglobinas Anormais/química
Hemoglobinas Anormais/metabolismo
[Mh] Termos MeSH secundário: 2,3-Difosfoglicerato/farmacologia
Regulação Alostérica
Heme/metabolismo
Hemoglobinas Anormais/genética
Seres Humanos
Técnicas In Vitro
Cinética
Modelos Moleculares
Simulação de Dinâmica Molecular
Oxigênio/metabolismo
Ácido Fítico/farmacologia
Domínios e Motivos de Interação entre Proteínas
Estrutura Quaternária de Proteína
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Hemoglobins, Abnormal); 138-81-8 (2,3-Diphosphoglycerate); 139735-68-5 (hemoglobin Coimbra); 42VZT0U6YR (Heme); 7IGF0S7R8I (Phytic Acid); S88TT14065 (Oxygen)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180212
[Lr] Data última revisão:
180212
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171205
[St] Status:MEDLINE


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[PMID]:27777071
[Au] Autor:Kong K; Hiraishi N; Nassar M; Otsuki M; Yiu CKY; Tagami J
[Ad] Endereço:Cariology and Operative Dentistry, Department of Oral Health Sciences, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo, Japan; General Dentistry, Roomchang Dental and Aesthetic Hospital, Phnom Penh, Cambodia.
[Ti] Título:Effect of phytic acid etchant on resin-dentin bonding: Monomer penetration and stability of dentin collagen.
[So] Source:J Prosthodont Res;61(3):251-258, 2017 Jul.
[Is] ISSN:2212-4632
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:PURPOSE: Phytic acid (IP6) works well as an etchant in dentin bonding to remove the smear layer due to its acidity and chelating effect. This study compared the etching effect of IP6 with phosphoric acid (PA) and ethylenediaminetetraacetic acid (EDTA) on resin-dentin bond strength, micromorphology of the etched dentin surface and nanoleakage formation along resin-dentin interfaces and compared the protecting effect against collagen degradation. METHODS: Dentin disks and flat dentin surfaces were obtained from extracted human teeth. Specimens were etched with 35% PA (15s), 0.5M EDTA (30s) or 1% IP6 (30s). The surfaces and longitudinal sections of the etched dentin disks were observed using field emission scanning electron microscope (FE-SEM). An etch-and-rinse adhesive was used to create composite build up-specimens for microtensile bond strength (µTBS) testing and nanoleakage observation. To evaluate the effect on collagen degradation, demineralized bovine root dentin blocks were challenged with bacterial collagenase and then observed under light microscope. RESULTS: PA- and EDTA- treated groups showed significantly lower µTBS when compared to IP6-treated group. PA showed distinct nanoleakage and severe collagen degradation. Only slight nanoleakage was detected in IP6 group. IP6 showed better effect than EDTA in preventing collagen degradation induced by bacterial collagenase. CONCLUSIONS: IP6 effectively removed the smear layer and etched dentin, providing high bond strength values and causing minimal nanoleakage and slight collagen degradation.
[Mh] Termos MeSH primário: Ataque Ácido Dentário
Colágeno
Colagem Dentária
Corrosão Dentária
Dentina
Ácido Fítico
Resinas Sintéticas
[Mh] Termos MeSH secundário: Ácido Edético
Seres Humanos
Ácidos Fosfóricos
Proteólise
Resistência à Tração
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Phosphoric Acids); 0 (Resins, Synthetic); 7IGF0S7R8I (Phytic Acid); 9007-34-5 (Collagen); 9G34HU7RV0 (Edetic Acid); E4GA8884NN (phosphoric acid)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171128
[Lr] Data última revisão:
171128
[Sb] Subgrupo de revista:D; IM
[Da] Data de entrada para processamento:161026
[St] Status:MEDLINE


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[PMID]:27776974
[Au] Autor:Shears SB; Baughman BM; Gu C; Nair VS; Wang H
[Ad] Endereço:Laboratory of Signal Transduction, National Institute of Environmental Health Sciences, National Institutes of Health, 101 T.W. Alexander Drive, Research Triangle Park, NC, 27709, USA. Electronic address: shears@niehs.nih.gov.
[Ti] Título:The significance of the 1-kinase/1-phosphatase activities of the PPIP5K family.
[So] Source:Adv Biol Regul;63:98-106, 2017 Jan.
[Is] ISSN:2212-4934
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The inositol pyrophosphates (diphosphoinositol polyphosphates), which include 1-InsP , 5-InsP , and InsP , are highly 'energetic' signaling molecules that play important roles in many cellular processes, particularly with regards to phosphate and bioenergetic homeostasis. Two classes of kinases synthesize the PP-InsPs: IP6Ks and PPIP5Ks. The significance of the IP6Ks - and their 5-InsP product - has been widely reported. However, relatively little is known about the biological significance of the PPIP5Ks. The purpose of this review is to provide an update on developments in our understanding of key features of the PPIP5Ks, which we believe strengthens the hypothesis that their catalytic activities serve important cellular functions. Central to this discussion is the recent discovery that the PPIP5K is a rare example of a single protein that catalyzes a kinase/phosphatase futile cycle.
[Mh] Termos MeSH primário: Hidrolases Anidrido Ácido/metabolismo
Fosfatos de Inositol/metabolismo
Fosfotransferases (Aceptor do Grupo Fosfato)/metabolismo
Ácido Fítico/metabolismo
[Mh] Termos MeSH secundário: Hidrolases Anidrido Ácido/genética
Motivos de Aminoácidos
Metabolismo Energético/genética
Regulação da Expressão Gênica
Seres Humanos
Fosfotransferases (Aceptor do Grupo Fosfato)/química
Fosfotransferases (Aceptor do Grupo Fosfato)/genética
Domínios Proteicos
Transdução de Sinais
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Inositol Phosphates); 0 (inositol heptakisphosphate); 7IGF0S7R8I (Phytic Acid); EC 2.7.4.- (Phosphotransferases (Phosphate Group Acceptor)); EC 2.7.4.21 (PPIP5K1 protein, human); EC 3.6.- (Acid Anhydride Hydrolases); EC 3.6.1.- (diphosphoinositol polyphosphate phosphohydrolase)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171128
[Lr] Data última revisão:
171128
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161026
[St] Status:MEDLINE


  6 / 2904 MEDLINE  
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[PMID]:28887383
[Au] Autor:Qi W; Manfield IW; Muench SP; Baker A
[Ad] Endereço:School of Molecular and Cellular Biology, Centre for Plant Sciences and Astbury Centre for Structural Molecular Biology, Faculty of Biological Sciences, University of Leeds, Leeds LS2 9JT, U.K.
[Ti] Título:AtSPX1 affects the AtPHR1-DNA-binding equilibrium by binding monomeric AtPHR1 in solution.
[So] Source:Biochem J;474(21):3675-3687, 2017 Oct 23.
[Is] ISSN:1470-8728
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Phosphorus is an essential macronutrient for plant growth and is deficient in ∼50% of agricultural soils. The transcription factor phosphate starvation response 1 (PHR1) plays a central role in regulating the expression of a subset of phosphate starvation-induced (PSI) genes through binding to a -acting DNA element termed P1BS (PHR1-binding sequences). In and rice, activity of AtPHR1/OsPHR2 is regulated in part by their downstream target SPX ( yg1, ho81, pr1) proteins through protein-protein interaction. Here, we provide kinetic and affinity data for interaction between AtPHR1 and P1BS sites. Using surface plasmon resonance, a tandem P1BS sequence showed ∼50-fold higher affinity for MBPAtdPHR1 (a fusion protein comprising the DNA-binding domain and coiled-coil domain of AtPHR1 fused to maltose-binding protein) than a single site. The affinity difference was largely reflected in a much slower dissociation rate from the 2× P1BS-binding site, suggesting an important role for protein co-operativity. Injection of AtSPX1 in the presence of phosphate or inositol hexakisphosphate (InsP6) failed to alter the MBPAtdPHR1-P1BS dissociation rate, while pre-mixing of these two proteins in the presence of either 5 mM Pi or 500 µM InsP6 resulted in a much lower DNA-binding signal from MBPAtdPHR1. These data suggest that, in the Pi-restored condition, AtSPX1 can bind to monomeric AtPHR1 in solution and therefore regulate PSI gene expression by tuning the AtPHR1-DNA-binding equilibrium. This Pi-dependent regulation of AtPHR1-DNA-binding equilibrium also generates a negative feedback loop on the expression of AtSPX1 itself, providing a tight control of PSI gene expression.
[Mh] Termos MeSH primário: Proteínas de Arabidopsis/química
Arabidopsis/química
DNA de Plantas/química
Proteínas Nucleares/química
Fatores de Transcrição/química
[Mh] Termos MeSH secundário: Arabidopsis/genética
Arabidopsis/metabolismo
Proteínas de Arabidopsis/genética
Proteínas de Arabidopsis/metabolismo
DNA de Plantas/genética
DNA de Plantas/metabolismo
Regulação da Expressão Gênica de Plantas/fisiologia
Proteínas Nucleares/genética
Proteínas Nucleares/metabolismo
Ácido Fítico/química
Ácido Fítico/metabolismo
Ligação Proteica
Domínios Proteicos
Fatores de Transcrição/genética
Fatores de Transcrição/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Arabidopsis Proteins); 0 (DNA, Plant); 0 (Nuclear Proteins); 0 (PHR1 protein, Arabidopsis); 0 (SPX1 protein, Arabidopsis); 0 (Transcription Factors); 7IGF0S7R8I (Phytic Acid)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171029
[Lr] Data última revisão:
171029
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170910
[St] Status:MEDLINE
[do] DOI:10.1042/BCJ20170522


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[PMID]:28764025
[Au] Autor:Magallanes-López AM; Hernandez-Espinosa N; Velu G; Posadas-Romano G; Ordoñez-Villegas VMG; Crossa J; Ammar K; Guzmán C
[Ad] Endereço:Global Wheat Program, International Maize and Wheat Improvement Center (CIMMYT), Apdo. Postal 6-641, Mexico DF, Mexico. Electronic address: anamaria_ml03@hotmail.com.
[Ti] Título:Variability in iron, zinc and phytic acid content in a worldwide collection of commercial durum wheat cultivars and the effect of reduced irrigation on these traits.
[So] Source:Food Chem;237:499-505, 2017 Dec 15.
[Is] ISSN:0308-8146
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Diets very rich in cereals have been associated with micronutrient malnutrition, and the biofortification of them, has been proposed as one of the best approaches to alleviate the problem. Durum wheat is one of the main sources of calories and protein in many developing countries. In this study, 46 durum varieties grown under full and reduced irrigation, were analyzed for micronutrients and phytate content to determine the potential bioavailability of the micronutrients. The variation was 25.7-40.5mg/kg for iron and of 24.8-48.8mg/kg for zinc. For phytate determination (0.462-0.952 %), a modified methodology was validated in order to reduce testing costs while speeding up testing time. Variation was detected for phytate:iron and zinc molar ratios (12.1-29.6 and 16.9-23.6, respectively). The results could be useful to generate varieties with appropriate levels of phytate and micronutrients, which can lead to the development of varieties rich in micronutrients to overcome malnutrition.
[Mh] Termos MeSH primário: Triticum
[Mh] Termos MeSH secundário: Disponibilidade Biológica
Ferro
Micronutrientes
Ácido Fítico
Zinco
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Micronutrients); 7IGF0S7R8I (Phytic Acid); E1UOL152H7 (Iron); J41CSQ7QDS (Zinc)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171106
[Lr] Data última revisão:
171106
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170803
[St] Status:MEDLINE


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[PMID]:28757134
[Au] Autor:Foster SR; Dilworth LL; Thompson RK; Alexander-Lindo RL; Omoruyi FO
[Ad] Endereço:Department of Basic Medical Sciences, Biochemistry Section, UWI, Mona, Jamaica. Electronic address: shadae.foster@mymona.uwi.edu.
[Ti] Título:Effects of combined inositol hexakisphosphate and inositol supplement on antioxidant activity and metabolic enzymes in the liver of streptozotocin-induced type 2 diabetic rats.
[So] Source:Chem Biol Interact;275:108-115, 2017 Sep 25.
[Is] ISSN:1872-7786
[Cp] País de publicação:Ireland
[La] Idioma:eng
[Ab] Resumo:Diabetes mellitus is associated with elevated reactive oxygen species, lipid abnormalities, reduced antioxidant activity and organ damage. This study examines the effects of combined inositol hexakisphosphate (IP6) and inositol supplement on antioxidant levels and other biochemical parameters in the liver of type 2 diabetic rats. Five groups of Sprague-Dawley rats were studied. Six rats were fed normal diet (non-diabetic control), while 24 rats were fed high-fat diet (HFD) for 4 weeks. Diabetes was induced in 18 of the rats fed HFD by intraperitoneal administration of streptozotocin. The diabetic rats were separated into three groups namely: combined IP6 and inositol, glibenclamide and diabetic control. The non-diabetic group fed high-fat diet was classified as a high-fat control group. For the final four weeks of the experiment, all rats were fed normal diet and given their respective treatment regimes. Hepatic antioxidant status, metabolic enzyme activity, lipid profile, peroxidative damage and liver histology, as well as, serum aminotransferase and alkaline phosphatase activities, and total bilirubin concentration were assessed. Treatment with combined IP6 and inositol supplement significantly increased liver reduced glutathione and high-density lipoprotein levels while liver triglyceride levels and serum alkaline phosphatase activity were significantly reduced by 27%, 50%, 38.5%, and 69.2% respectively compared to the diabetic control. Hepatic superoxide dismutase, catalase, glucose-6-phosphate dehydrogenase activities were significantly upregulated by 55%, 26% and 53% respectively in the diabetic rats treated with combined IP6 and inositol compared to the diabetic control. Combined IP6 and inositol treatment resulted in the preservation of liver cell integrity and improved antioxidant status in type 2 diabetic rats.
[Mh] Termos MeSH primário: Antioxidantes/metabolismo
Suplementos Nutricionais
Inositol
Fígado/efeitos dos fármacos
Ácido Fítico/farmacologia
Regulação para Cima/efeitos dos fármacos
[Mh] Termos MeSH secundário: Animais
Bilirrubina/sangue
Diabetes Mellitus Experimental/induzido quimicamente
Diabetes Mellitus Experimental/patologia
Ativação Enzimática/efeitos dos fármacos
Glucosefosfato Desidrogenase/metabolismo
Glutationa/metabolismo
Lipoproteínas HDL/metabolismo
Fígado/metabolismo
Fígado/patologia
Masculino
Estresse Oxidativo/efeitos dos fármacos
Oxirredutases/metabolismo
Ratos
Ratos Sprague-Dawley
Estreptozocina/toxicidade
Triglicerídeos/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antioxidants); 0 (Lipoproteins, HDL); 0 (Triglycerides); 4L6452S749 (Inositol); 5W494URQ81 (Streptozocin); 7IGF0S7R8I (Phytic Acid); EC 1.- (Oxidoreductases); EC 1.1.1.49 (Glucosephosphate Dehydrogenase); GAN16C9B8O (Glutathione); RFM9X3LJ49 (Bilirubin)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170926
[Lr] Data última revisão:
170926
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170801
[St] Status:MEDLINE


  9 / 2904 MEDLINE  
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[PMID]:28737762
[Au] Autor:Zhang ZM; Ma KW; Gao L; Hu Z; Schwizer S; Ma W; Song J
[Ad] Endereço:Department of Biochemistry, University of California, Riverside, California 92521, USA.
[Ti] Título:Mechanism of host substrate acetylation by a YopJ family effector.
[So] Source:Nat Plants;3:17115, 2017 Jul 24.
[Is] ISSN:2055-0278
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The Yersinia outer protein J (YopJ) family of bacterial effectors depends on a novel acetyltransferase domain to acetylate signalling proteins from plant and animal hosts. However, the underlying mechanism is unclear. Here, we report the crystal structures of PopP2, a YopJ effector produced by the plant pathogen Ralstonia solanacearum, in complex with inositol hexaphosphate (InsP ), acetyl-coenzyme A (AcCoA) and/or substrate Resistance to Ralstonia solanacearum 1 (RRS1-R) . PopP2 recognizes the WRKYGQK motif of RRS1-R to position a targeted lysine in the active site for acetylation. Importantly, the PopP2-RRS1-R association is allosterically regulated by InsP binding, suggesting a previously unidentified role of the eukaryote-specific cofactor in substrate interaction. Furthermore, we provide evidence for the reaction intermediate of PopP2-mediated acetylation, an acetyl-cysteine covalent adduct, lending direct support to the 'ping-pong'-like catalytic mechanism proposed for YopJ effectors. Our study provides critical mechanistic insights into the virulence activity of YopJ class of acetyltransferases.
[Mh] Termos MeSH primário: Proteínas de Bactérias/química
Yersinia/metabolismo
[Mh] Termos MeSH secundário: Acetilcoenzima A/química
Acetilação
Proteínas de Bactérias/metabolismo
Domínio Catalítico
Cristalografia por Raios X
Modelos Moleculares
Ácido Fítico/química
Conformação Proteica
Ralstonia solanacearum/metabolismo
Sistemas de Secreção Tipo III
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Type III Secretion Systems); 0 (YopP protein, Yersinia); 72-89-9 (Acetyl Coenzyme A); 7IGF0S7R8I (Phytic Acid)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171113
[Lr] Data última revisão:
171113
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170725
[St] Status:MEDLINE
[do] DOI:10.1038/nplants.2017.115


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[PMID]:28651042
[Au] Autor:Özkaya H; Özkaya B; Duman B; Turksoy S
[Ad] Endereço:Faculty of Engineering, Department of Food Engineering, Ankara University , Golbasi Campus, Golbasi, Ankara TR-06830, Turkey.
[Ti] Título:Effect of Dephytinization by Fermentation and Hydrothermal Autoclaving Treatments on the Antioxidant Activity, Dietary Fiber, and Phenolic Content of Oat Bran.
[So] Source:J Agric Food Chem;65(28):5713-5719, 2017 Jul 19.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Fermentation and hydrothermal methods were tested to reduce the phytic acid (PA) content of oat bran, and the effects of these methods on the dietary fiber (DF) and total phenolic (TP) contents as well as the antioxidant activity (AA) were also investigated. Fermentation with 6% yeast and for 6 h resulted in 88.2% reduction in PA content, while it only resulted in 32.5% reduction in the sample incubated for 6 h without yeast addition. The PA loss in autoclaved oat bran sample (1.5 h, pH 4.0) was 95.2% while it was 41.8% at most in the sample autoclaved without pH adjustment. In both methods, soluble, insoluble, and total DF contents of samples were remarkably higher than the control samples. Also for TP in the oat bran samples, both processes led to 17% and 39% increases, respectively, while AA values were 8% and 15%, respectively. Among all samples, the autoclaving process resulted in the lowest PA and the greatest amount of bioactive compounds.
[Mh] Termos MeSH primário: Antioxidantes/química
Avena/química
Fibras na Dieta/metabolismo
Fenóis/química
Saccharomyces cerevisiae/metabolismo
[Mh] Termos MeSH secundário: Antioxidantes/metabolismo
Avena/metabolismo
Avena/microbiologia
Fibras na Dieta/análise
Fibras na Dieta/microbiologia
Fermentação
Temperatura Alta
Oxirredução
Fenóis/metabolismo
Ácido Fítico/química
Ácido Fítico/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antioxidants); 0 (Dietary Fiber); 0 (Phenols); 7IGF0S7R8I (Phytic Acid)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170627
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jafc.7b01698



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