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[PMID]: | 29241319 |
[Au] Autor: | Chen H; Huang J; Shi X; Li Y; Liu Y |
[Ad] Endereço: | School of Food and Biological Engineering, Shaanxi University of Science and Technology Xi?an,China. |
[Ti] Título: | Effects of six substances on the growth and freeze-drying of Lactobacillus delbrueckii subsp. bulgaricus. |
[So] Source: | Acta Sci Pol Technol Aliment;16(4):403-412, 2017 Oct-Dec. | [Is] ISSN: | 1898-9594 |
[Cp] País de publicação: | Poland |
[La] Idioma: | eng |
[Ab] Resumo: | BACKGROUND: The efficacy of Lactobacillus delbrueckii subsp. bulgaricus as starter cultures for the dairy industry depends largely on the number of viable and active cells. Freeze-drying is the most convenient and successful method to preserve the bacterial cells. However, not all strains survived during freeze-drying. METHODS: The effects of six substances including NaCl, sorbitol, mannitol, mannose, sodium glutamate, betaine added to the MRS medium on the growth and freeze-drying survival rate and viable counts of Lb. delbrueckii subsp. bulgaricus were studied through a single-factor test and Plackett-Burman design. Subsequently, the optimum freeze-drying conditions of Lb. delbrueckii subsp. bulgaricus were determined. RESULTS: Lb. delbrueckii subsp. bulgaricus survival rates were up to the maximum of 42.7%, 45.4%, 23.6%, while the concentrations of NaCl, sorbitol, sodium glutamate were 0.6%, 0.15%, 0.09%, respectively. In the optimum concentration, the viable counts in broth is 6.1, 6.9, 5.13 (×108 CFU/mL), respectively; the viable counts in freeze-drying power are 3.09, 5.2, 2.7 (×1010 CFU/g), respectively. CONCLUSIONS: Three antifreeze factors including NaCl, sorbitol, sodium glutamate have a positive effect on the growth and freeze-drying of Lb. delbrueckii subsp. bulgaricus. The results are beneficial for developing Lb. delbrueckii subsp. bulgaricus. |
[Mh] Termos MeSH primário: |
Crioprotetores/farmacologia Aditivos Alimentares/farmacologia Liofilização Lactobacillus delbrueckii
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[Mh] Termos MeSH secundário: |
Betaína/farmacologia Lactobacillus delbrueckii/efeitos dos fármacos Lactobacillus delbrueckii/crescimento & desenvolvimento Manitol/farmacologia Viabilidade Microbiana Cloreto de Sódio/farmacologia Glutamato de Sódio/farmacologia Sorbitol/farmacologia Iogurte/microbiologia
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[Pt] Tipo de publicação: | JOURNAL ARTICLE |
[Nm] Nome de substância:
| 0 (Cryoprotective Agents); 0 (Food Additives); 3OWL53L36A (Mannitol); 3SCV180C9W (Betaine); 451W47IQ8X (Sodium Chloride); 506T60A25R (Sorbitol); W81N5U6R6U (Sodium Glutamate) |
[Em] Mês de entrada: | 1802 |
[Cu] Atualização por classe: | 180208 |
[Lr] Data última revisão:
| 180208 |
[Sb] Subgrupo de revista: | IM |
[Da] Data de entrada para processamento: | 171216 |
[St] Status: | MEDLINE |
[do] DOI: | 10.17306/J.AFS.0512 |
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