Base de dados : MEDLINE
Pesquisa : D02.047.407 [Categoria DeCS]
Referências encontradas : 18227 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 1823 ir para página                         

  1 / 18227 MEDLINE  
              next record last record
seleciona
para imprimir
Fotocópia
[PMID]:29446294
[Au] Autor:Efimova NV; Rukavishnikov VS; Pankov VA; Perezhogin AN; Shayakhmetov SF; Meshchakova NM; Lisetskaya LG
[Ti] Título:[Assessment of carcinogenic risks to workers of the main enterprises of the Irkutsk region].
[So] Source:Gig Sanit;95(12):1163-7, 2016.
[Is] ISSN:0016-9900
[Cp] País de publicação:Russia (Federation)
[La] Idioma:rus
[Ab] Resumo:The purpose of research is the assessment of the individual cancer risk (ICR) for workers of the basic occupations in key branches of industry of the Irkutsk region. There was executed the calculation of ICR levels for workers of the basic occupations of the aircraft industry, aluminum smelters and vinyl chloride production plants. The estimation of the exposure for workers was carried out according to long-term time-weighted average concentrations in the air of the working area, for the population - on annual average concentrations in the ambient air. To assess the risk that is not associated with the profession, the dose was calculated for the period of life (70 years). When calculating the toxicant doses in the working area there were used the "standard" indices ofpulmonary ventilation for adults, body weight, the work experience in the contact with carcinogens of 30 years, the number of days in the contact of 240, the duration of the working time 8 or 12 hours (in accordance with the working hours) duration. ICR for the Irkutsk population amounted of 3.08E-04, in Shelekhov - 4.8E-05, Sayansk - 1.1E-05. The amount of risk depends on the content offormaldehyde in all territories and chromium VI in cities of Irkutsk and Shelekhov. ICR for workers of basic occupations of studied plants in dozens of times are higher than for the urban population. Priority carcinogens are: chromium VI, nickel, formaldehyde, silicon dioxide -for the aircraft plant employees; 1,2-dichloretan, vinyl chloride - for the workers of vinyl chloride production plant; benzopyrene - for the aluminum smelter workers.
[Mh] Termos MeSH primário: Poluentes Ocupacionais do Ar
Carcinogênese/induzido quimicamente
Cromo
Formaldeído
Indústria Manufatureira
Cloreto de Vinil
[Mh] Termos MeSH secundário: Poluentes Ocupacionais do Ar/análise
Poluentes Ocupacionais do Ar/toxicidade
Carcinógenos Ambientais/análise
Carcinógenos Ambientais/toxicidade
Cromo/análise
Cromo/toxicidade
Formaldeído/análise
Formaldeído/toxicidade
Seres Humanos
Indústria Manufatureira/métodos
Indústria Manufatureira/normas
Exposição Ocupacional/efeitos adversos
Exposição Ocupacional/análise
Exposição Ocupacional/prevenção & controle
Medição de Risco/métodos
Medição de Risco/estatística & dados numéricos
Fatores de Risco
Sibéria/epidemiologia
Tempo
Cloreto de Vinil/análise
Cloreto de Vinil/toxicidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Air Pollutants, Occupational); 0 (Carcinogens, Environmental); 0R0008Q3JB (Chromium); 18540-29-9 (chromium hexavalent ion); 1HG84L3525 (Formaldehyde); WD06X94M2D (Vinyl Chloride)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180216
[St] Status:MEDLINE


  2 / 18227 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
[PMID]:29269543
[Ti] Título:Clarity on use of formaldehyde in fish.
[So] Source:Vet Rec;181(25):670, 2017 12 23.
[Is] ISSN:2042-7670
[Cp] País de publicação:England
[La] Idioma:eng
[Mh] Termos MeSH primário: Aquicultura
Peixes
Formaldeído/uso terapêutico
Guias de Prática Clínica como Assunto
[Mh] Termos MeSH secundário: Animais
Seres Humanos
Sociedades
Reino Unido
Medicina Veterinária
[Pt] Tipo de publicação:NEWS
[Nm] Nome de substância:
1HG84L3525 (Formaldehyde)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180308
[Lr] Data última revisão:
180308
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171223
[St] Status:MEDLINE


  3 / 18227 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28742856
[Au] Autor:Yakovleva A; Plieskatt JL; Jensen S; Humeida R; Lang J; Li G; Bracci P; Silver S; Bethony JM
[Ad] Endereço:Department of Microbiology, Immunology and Tropical Medicine, The George Washington University, Washington DC, United States of America.
[Ti] Título:Fit for genomic and proteomic purposes: Sampling the fitness of nucleic acid and protein derivatives from formalin fixed paraffin embedded tissue.
[So] Source:PLoS One;12(7):e0181756, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The demand for nucleic acid and protein derivatives from formalin-fixed paraffin-embedded (FFPE) tissue has greatly increased due to advances in extraction and purification methods, making these derivatives available for numerous genomic and proteomic platforms. Previously, DNA, RNA, microRNA (miRNA), or protein derived from FFPE tissue blocks were considered "unfit" for such platforms, as the process of tissue immobilization by FFPE resulted in cross-linked, fragmented, and chemically modified macromolecules. We conducted a systematic examination of nucleic acids and proteins co-extracted from 118 FFPE blocks sampled from the AIDS and Cancer Specimen Resource (ACSR) at The George Washington University after stratification by storage duration and the three most common tumor tissue types at the ACSR (adenocarcinoma, squamous cell carcinoma, and papillary carcinoma). DNA, RNA, miRNA, and protein could be co-extracted from 98% of the FFPE blocks sampled, with DNA and miRNA "fit" for diverse genomic purposes including sequencing. While RNA was the most labile of the FFPE derivatives, especially when assessed by RNA integrity number (RIN), it was still "fit" for genomic methods that use smaller sequence lengths, e.g., quantitative PCR. While more than half of the protein derivatives were fit for proteomic purposes, our analyses indicated a significant interaction effect on the absorbance values for proteins derived from FFPE, implying that storage duration may affect protein derivatives differently by tumor tissue type. The mean absorbance value for proteins derived from more recently stored FFPE was greater than protein derived from older FFPE, with the exception of adenocarcinoma tissue. Finally, the fitness of one type of derivative was weakly associated with the fitness of derivatives co-extracted from the same FFPE block. The current study used several novel quality assurance approaches and metrics to show that archival FFPE tissue blocks are a valuable resource for contemporary genomic and proteomic platforms.
[Mh] Termos MeSH primário: Genômica/métodos
Ácidos Nucleicos/análise
Inclusão em Parafina/métodos
Proteínas/análise
Proteômica/métodos
[Mh] Termos MeSH secundário: Adenocarcinoma/química
Adenocarcinoma/genética
Carcinoma Papilar/química
Carcinoma Papilar/genética
Carcinoma de Células Escamosas/química
Carcinoma de Células Escamosas/genética
DNA/análise
DNA/genética
DNA de Neoplasias/análise
DNA de Neoplasias/genética
Formaldeído/uso terapêutico
Seres Humanos
MicroRNAs/análise
MicroRNAs/genética
Ácidos Nucleicos/genética
Proteínas/genética
RNA/análise
RNA/genética
Fatores de Tempo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Neoplasm); 0 (MicroRNAs); 0 (Nucleic Acids); 0 (Proteins); 1HG84L3525 (Formaldehyde); 63231-63-0 (RNA); 9007-49-2 (DNA)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:180209
[Lr] Data última revisão:
180209
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170726
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0181756


  4 / 18227 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28468229
[Au] Autor:Kuzucu I; Beriat GK; Ezerarslan H; Ozdemir S; Kocaturk S
[Ad] Endereço:Department of Otolaryngology Head and Neck Surgery, Ufuk University, Ankara, Turkey.
[Ti] Título:Effects of the Autologous Platelet-Rich Plasma in Nasal Pack on Postoperative Quality of Life.
[So] Source:J Craniofac Surg;28(3):e299-e302, 2017 May.
[Is] ISSN:1536-3732
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:AIM: The authors aimed to research the effects of autologous platelet-rich plasma, which is known for the positive effects on wound healing, on postoperatif living quality of the patients after using on nasal surgery. METHODS: Patients who undergo nasal operation according to randomized patient's choice have been injected with saline merocel pack and platelet-rich plasma (PRP) in their nasal path. Postoperative is followed by 1 month; Nose Obstruction Symptom Evaluation (NOSE) scala score, bleeding, pain, and crust rate have been compared between 2 groups. RESULTS: As a result of the 1 month follow-up, it was seen that the treatment group experienced better results in terms of NOSE scale than the control group patients and this was significant difference in terms of NOSE. Less bleeding and crust was seen on the treatment group patients than the control group patients and statically significant difference was found. In terms of visual analogue scale score no statically significant difference was found between 2 groups. CONCLUSION: According to the authors' study on PRP, the authors found out that by using PRP wound can be healed faster in early period of postoperative. It was seen with the authors' research that on postoperative early period crusts, bleeding and postoperative complaints are lesser.
[Mh] Termos MeSH primário: Bandagens
Plasma Rico em Plaquetas
Cuidados Pós-Operatórios/métodos
Qualidade de Vida
Rinoplastia
[Mh] Termos MeSH secundário: Adolescente
Adulto
Feminino
Seguimentos
Formaldeído/uso terapêutico
Hemostáticos/uso terapêutico
Seres Humanos
Masculino
Meia-Idade
Álcool de Polivinil/uso terapêutico
Cuidados Pós-Operatórios/instrumentação
Hemorragia Pós-Operatória/prevenção & controle
Período Pós-Operatório
Método Simples-Cego
Resultado do Tratamento
Cicatrização
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE; RANDOMIZED CONTROLLED TRIAL
[Nm] Nome de substância:
0 (Hemostatics); 1HG84L3525 (Formaldehyde); 26876-25-5 (polyvinyl alcohol formaldehyde foam); 9002-89-5 (Polyvinyl Alcohol)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180202
[Lr] Data última revisão:
180202
[Sb] Subgrupo de revista:D
[Da] Data de entrada para processamento:170505
[St] Status:MEDLINE
[do] DOI:10.1097/SCS.0000000000003664


  5 / 18227 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28463494
[Au] Autor:Rohlhill J; Sandoval NR; Papoutsakis ET
[Ad] Endereço:Department of Chemical & Biomolecular Engineering and the Delaware Biotechnology Institute, University of Delaware , Newark, Delaware 19711, United States.
[Ti] Título:Sort-Seq Approach to Engineering a Formaldehyde-Inducible Promoter for Dynamically Regulated Escherichia coli Growth on Methanol.
[So] Source:ACS Synth Biol;6(8):1584-1595, 2017 Aug 18.
[Is] ISSN:2161-5063
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Tight and tunable control of gene expression is a highly desirable goal in synthetic biology for constructing predictable gene circuits and achieving preferred phenotypes. Elucidating the sequence-function relationship of promoters is crucial for manipulating gene expression at the transcriptional level, particularly for inducible systems dependent on transcriptional regulators. Sort-seq methods employing fluorescence-activated cell sorting (FACS) and high-throughput sequencing allow for the quantitative analysis of sequence-function relationships in a robust and rapid way. Here we utilized a massively parallel sort-seq approach to analyze the formaldehyde-inducible Escherichia coli promoter (P ) with single-nucleotide resolution. A library of mutated formaldehyde-inducible promoters was cloned upstream of gfp on a plasmid. The library was partitioned into bins via FACS on the basis of green fluorescent protein (GFP) expression level, and mutated promoters falling into each expression bin were identified with high-throughput sequencing. The resulting analysis identified two 19 base pair repressor binding sites, one upstream of the -35 RNA polymerase (RNAP) binding site and one overlapping with the -10 site, and assessed the relative importance of each position and base therein. Key mutations were identified for tuning expression levels and were used to engineer formaldehyde-inducible promoters with predictable activities. Engineered variants demonstrated up to 14-fold lower basal expression, 13-fold higher induced expression, and a 3.6-fold stronger response as indicated by relative dynamic range. Finally, an engineered formaldehyde-inducible promoter was employed to drive the expression of heterologous methanol assimilation genes and achieved increased biomass levels on methanol, a non-native substrate of E. coli.
[Mh] Termos MeSH primário: Proliferação Celular/genética
Proteínas de Escherichia coli/genética
Escherichia coli/fisiologia
Regulação Bacteriana da Expressão Gênica/genética
Engenharia Metabólica/métodos
Metanol/metabolismo
Regiões Promotoras Genéticas/genética
[Mh] Termos MeSH secundário: Algoritmos
Escherichia coli/citologia
Escherichia coli/efeitos dos fármacos
Citometria de Fluxo/métodos
Formaldeído/administração & dosagem
Regulação Bacteriana da Expressão Gênica/efeitos dos fármacos
Sequenciamento de Nucleotídeos em Larga Escala/métodos
Ativação Transcricional/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Escherichia coli Proteins); 1HG84L3525 (Formaldehyde); Y4S76JWI15 (Methanol)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180201
[Lr] Data última revisão:
180201
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170503
[St] Status:MEDLINE
[do] DOI:10.1021/acssynbio.7b00114


  6 / 18227 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29324749
[Au] Autor:Mendel JR; Baig SA; Hall MG; Jeong M; Byron MJ; Morgan JC; Noar SM; Ribisl KM; Brewer NT
[Ad] Endereço:Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, NC, United States of America.
[Ti] Título:Brand switching and toxic chemicals in cigarette smoke: A national study.
[So] Source:PLoS One;13(1):e0189928, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:INTRODUCTION: US law requires disclosure of quantities of toxic chemicals (constituents) in cigarette smoke by brand and sub-brand. This information may drive smokers to switch to cigarettes with lower chemical quantities, under the misperception that doing so can reduce health risk. We sought to understand past brand-switching behavior and whether learning about specific chemicals in cigarette smoke increases susceptibility to brand switching. METHODS: Participants were US adult smokers surveyed by phone (n = 1,151, probability sample) and online (n = 1,561, convenience sample). Surveys assessed whether smokers had ever switched cigarette brands or styles to reduce health risk and about likelihood of switching if the smoker learned their brand had more of a specific chemical than other cigarettes. Chemicals presented were nicotine, carbon monoxide, lead, formaldehyde, arsenic, and ammonia. RESULTS: Past brand switching to reduce health risk was common among smokers (43% in phone survey, 28% in online survey). Smokers who were female, over 25, and current "light" cigarette users were more likely to have switched brands to reduce health risks (all p < .05). Overall, 61-92% of smokers were susceptible to brand switching based on information about particular chemicals. In both samples, lead, formaldehyde, arsenic, and ammonia led to more susceptibility to switch than nicotine (all p < .05). CONCLUSIONS: Many US smokers have switched brands or styles to reduce health risks. The majority said they might or would definitely switch brands if they learned their cigarettes had more of a toxic chemical than other brands. Brand switching is a probable unintended consequence of communications that show differences in smoke chemicals between brands.
[Mh] Termos MeSH primário: Fumaça/análise
Produtos do Tabaco/análise
[Mh] Termos MeSH secundário: Adolescente
Adulto
Idoso
Amônia/análise
Arsênico/análise
Monóxido de Carbono/análise
Feminino
Formaldeído/análise
Seres Humanos
Chumbo/análise
Masculino
Meia-Idade
Nicotina/análise
Estados Unidos
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Smoke); 1HG84L3525 (Formaldehyde); 2P299V784P (Lead); 6M3C89ZY6R (Nicotine); 7664-41-7 (Ammonia); 7U1EE4V452 (Carbon Monoxide); N712M78A8G (Arsenic)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180130
[Lr] Data última revisão:
180130
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180112
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0189928


  7 / 18227 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29304043
[Au] Autor:Bosson-Rieutort D; de Gaudemaris R; Bicout DJ
[Ad] Endereço:Grenoble Alpes University / CNRS / TIMC-IMAG UMR 5525 (EPSP team - Environment and Health Prediction of Populations), Grenoble, France.
[Ti] Título:The spectrosome of occupational health problems.
[So] Source:PLoS One;13(1):e0190196, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Given the increased prevalence of cancer, respiratory diseases, and reproductive disorders, for which multifactorial origins are strongly suspected, the impact of the environment on the population represents a substantial public health challenge. Surveillance systems have become an essential public health decision-making tool. Networks have been constructed to facilitate the development of analyses of the multifactorial aspects of the relationships between occupational contexts and health. The aim of this study is to develop and present an approach for the optimal exploitation of observational databases to describe and improve the understanding of the (occupational) environment-health relationships, taking into account key multifactorial aspects. We have developed a spectral analysis (SA) approach that takes into account both the multi-exposure and dynamic natures of occupational health problems (OHPs) and related associations. The main results of this paper are to present the construction method of the "spectrum" and "spectrosome" of OHPs (range and structured list of occupational exposures) and describe the information contained therein with an illustrative example. The approach is illustrated using the case of non-Hodgkin lymphoma (NHL) from the French National Occupational Diseases Surveillance and Prevention Network database as a working example of an occupational disease. We found that the NHL spectrum includes 40 sets of occupational exposures characterized by important multi-exposures, especially solvent combinations or pesticide combinations, but also specific exposures such as polycyclic aromatic hydrocarbons, formaldehyde and ionizing radiation. These findings may be useful for surveillance and the assessment of occupational exposure related to health risks.
[Mh] Termos MeSH primário: Doenças Profissionais/epidemiologia
[Mh] Termos MeSH secundário: Formaldeído/toxicidade
Seres Humanos
Doenças Profissionais/classificação
Hidrocarbonetos Aromáticos Policíclicos/toxicidade
Vigilância da População
Prevalência
Radiação Ionizante
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Polycyclic Aromatic Hydrocarbons); 1HG84L3525 (Formaldehyde)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180129
[Lr] Data última revisão:
180129
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180106
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0190196


  8 / 18227 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29186505
[Au] Autor:Oudelaar AM; Davies JOJ; Downes DJ; Higgs DR; Hughes JR
[Ad] Endereço:Medical Research Council (MRC) Molecular Haematology Unit, Weatherall Institute of Molecular Medicine, University of Oxford, Oxford OX3 9DS, UK.
[Ti] Título:Robust detection of chromosomal interactions from small numbers of cells using low-input Capture-C.
[So] Source:Nucleic Acids Res;45(22):e184, 2017 Dec 15.
[Is] ISSN:1362-4962
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Chromosome conformation capture (3C) techniques are crucial to understanding tissue-specific regulation of gene expression, but current methods generally require large numbers of cells. This hampers the investigation of chromatin architecture in rare cell populations. We present a new low-input Capture-C approach that can generate high-quality 3C interaction profiles from 10 000-20 000 cells, depending on the resolution used for analysis. We also present a PCR-free, sequencing-free 3C technique based on NanoString technology called C-String. By comparing C-String and Capture-C interaction profiles we show that the latter are not skewed by PCR amplification. Furthermore, we demonstrate that chromatin interactions detected by Capture-C do not depend on the degree of cross-linking by performing experiments with varying formaldehyde concentrations.
[Mh] Termos MeSH primário: Cromatina/metabolismo
Cromossomos/metabolismo
Técnicas Genéticas
Nanotecnologia/métodos
[Mh] Termos MeSH secundário: Animais
Contagem de Células
Células Cultivadas
Cromatina/química
Cromatina/genética
Cromossomos/química
Cromossomos/genética
Feminino
Formaldeído/química
Regulação da Expressão Gênica
Camundongos Endogâmicos C57BL
Conformação Molecular
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Chromatin); 1HG84L3525 (Formaldehyde)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180115
[Lr] Data última revisão:
180115
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171130
[St] Status:MEDLINE
[do] DOI:10.1093/nar/gkx1194


  9 / 18227 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29172492
[Au] Autor:Xiang Y; Ru X; Shi J; Song J; Zhao H; Liu Y; Guo D; Lu X
[Ad] Endereço:Research Center for Engineering Technology of Polymeric Composites of Shanxi Province, School of Materials Science and Engineering, North University of China , Taiyuan 030051, China.
[Ti] Título:Preparation and Properties of a Novel Semi-IPN Slow-Release Fertilizer with the Function of Water Retention.
[So] Source:J Agric Food Chem;65(50):10851-10858, 2017 Dec 20.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:A new semi-interpenetrating polymer network (semi-IPN) slow-release fertilizer (SISRF) with water absorbency, based on the kaolin-g-poly(acrylic acid-co-acrylic amide) (kaolin-g-P(AA-co-AM)) network and linear urea-formaldehyde oligomers (UF), was prepared by solution polymerization. Nutrients phosphorus and potassium were supplied by adding dipotassium hydrogen phosphate during the preparation process. The structure and properties of SISRF were characterized by various characterization methods. SISRF showed excellent water absorbency of 68 g g in tap water. The slow-release behavior of nutrients and water-retention capacity of SISRF were also measured. Meanwhile, the swelling kinetics was well described by a pseudo-second-order kinetics model. Results suggested the formation of SISRF with simultaneously good slow-release and water-retention capacity, which was expected to apply in modern agriculture and horticulture.
[Mh] Termos MeSH primário: Preparações de Ação Retardada/síntese química
Fertilizantes/análise
Polímeros/síntese química
Água/química
[Mh] Termos MeSH secundário: Resinas Acrílicas/química
Amidas/química
Reagentes para Ligações Cruzadas/química
Preparações de Ação Retardada/química
Formaldeído/química
Polimerização
Polímeros/química
Ureia/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Acrylic Resins); 0 (Amides); 0 (Cross-Linking Reagents); 0 (Delayed-Action Preparations); 0 (Fertilizers); 0 (Polymers); 059QF0KO0R (Water); 1HG84L3525 (Formaldehyde); 4Q93RCW27E (carbopol 940); 8W8T17847W (Urea)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180110
[Lr] Data última revisão:
180110
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171128
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jafc.7b03827


  10 / 18227 MEDLINE  
              first record previous record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29180174
[Au] Autor:Nagahashi M; Shimada Y; Ichikawa H; Nakagawa S; Sato N; Kaneko K; Homma K; Kawasaki T; Kodama K; Lyle S; Takabe K; Wakai T
[Ad] Endereço:Division of Digestive and General Surgery, Niigata University Graduate School of Medical and Dental Sciences, Niigata City, Niigata, Japan. Electronic address: mnagahashi@med.niigata-u.ac.jp.
[Ti] Título:Formalin-fixed paraffin-embedded sample conditions for deep next generation sequencing.
[So] Source:J Surg Res;220:125-132, 2017 Dec.
[Is] ISSN:1095-8673
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:INTRODUCTION: Precision medicine is only possible in oncology practice if targetable genes in fragmented DNA, such as DNA from formalin-fixed paraffin-embedded (FFPE) samples, can be sequenced using next generation sequencing (NGS). The aim of this study was to examine the quality and quantity of DNA from FFPE cancerous tissue samples from surgically resected and biopsy specimens. METHODS: DNA was extracted from unstained FFPE tissue sections prepared from surgically resected specimens of breast, colorectal and gastric cancer, and biopsy specimens of breast cancer. A total quantity of DNA ≥60 ng from a sample was considered adequate for NGS. The DNA quality was assessed by Q-ratios, with a Q-ratio >0.1 considered sufficient for NGS. RESULTS: The Q-ratio for DNA from FFPE tissue processed with neutral-buffered formalin was significantly better than that processed with unbuffered formalin. All Q-ratios for DNA from breast, colorectal and gastric cancer samples indicated DNA levels sufficient for NGS. DNA extracted from gastric cancer FFPE samples prepared within the last 7 years is suitable for NGS analysis, whereas those older than 7 years may not be suitable. Our data suggested that adequate amounts of DNA can be extracted from FFPE samples, not only of surgically resected tissue but also of biopsy specimens. CONCLUSIONS: The type of formalin used for fixation and the time since FFPE sample preparation affect DNA quality. Sufficient amounts of DNA can be extracted from FFPE samples of both surgically resected and biopsy tissue, thus expanding the potential diagnostic uses of NGS in a clinical setting.
[Mh] Termos MeSH primário: DNA/isolamento & purificação
Formaldeído/química
Sequenciamento de Nucleotídeos em Larga Escala
Análise de Sequência de DNA
Manejo de Espécimes/métodos
[Mh] Termos MeSH secundário: Biópsia
Neoplasias da Mama/patologia
Neoplasias da Mama/cirurgia
Neoplasias Colorretais/patologia
Neoplasias Colorretais/cirurgia
Feminino
Seres Humanos
Inclusão em Parafina
Neoplasias Gástricas/patologia
Neoplasias Gástricas/cirurgia
Fatores de Tempo
[Pt] Tipo de publicação:JOURNAL ARTICLE; VALIDATION STUDIES
[Nm] Nome de substância:
1HG84L3525 (Formaldehyde); 9007-49-2 (DNA)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171219
[Lr] Data última revisão:
171219
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171129
[St] Status:MEDLINE



página 1 de 1823 ir para página                         
   


Refinar a pesquisa
  Base de dados : MEDLINE Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde