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[PMID]:28760934
[Au] Autor:Spinler JK; Auchtung J; Brown A; Boonma P; Oezguen N; Ross CL; Luna RA; Runge J; Versalovic J; Peniche A; Dann SM; Britton RA; Haag A; Savidge TC
[Ad] Endereço:Department of Pathology & Immunology, Baylor College of Medicine, Houston, Texas, USA spinler@bcm.edu.
[Ti] Título:Next-Generation Probiotics Targeting Clostridium difficile through Precursor-Directed Antimicrobial Biosynthesis.
[So] Source:Infect Immun;85(10), 2017 Oct.
[Is] ISSN:1098-5522
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Integration of antibiotic and probiotic therapy has the potential to lessen the public health burden of antimicrobial-associated diseases. infection (CDI) represents an important example where the rational design of next-generation probiotics is being actively pursued to prevent disease recurrence. Because intrinsic resistance to clinically relevant antibiotics used to treat CDI (vancomycin, metronidazole, and fidaxomicin) is a desired trait in such probiotic species, we screened several bacteria and identified to be a promising candidate for adjunct therapy. Human-derived bacteria convert glycerol to the broad-spectrum antimicrobial compound reuterin. When supplemented with glycerol, strains carrying the gene locus were potent reuterin producers, with 17938 inhibiting growth at a level on par with the level of growth inhibition by vancomycin. Targeted mutations and complementation studies identified reuterin to be the precursor-induced antimicrobial agent. Pathophysiological relevance was demonstrated when the codelivery of with glycerol was effective against colonization in complex human fecal microbial communities, whereas treatment with either glycerol or alone was ineffective. A global unbiased microbiome and metabolomics analysis independently confirmed that glycerol precursor delivery with elicited changes in the composition and function of the human microbial community that preferentially targets outgrowth and toxicity, a finding consistent with glycerol fermentation and reuterin production. Antimicrobial resistance has thus been successfully exploited in the natural design of human microbiome evasion of , and this method may provide a prototypic precursor-directed probiotic approach. Antibiotic resistance and substrate bioavailability may therefore represent critical new determinants of probiotic efficacy in clinical trials.
[Mh] Termos MeSH primário: Antibacterianos/biossíntese
Infecções por Clostridium/prevenção & controle
Clostridium difficile/crescimento & desenvolvimento
Gliceraldeído/análogos & derivados
Glicerol/administração & dosagem
Lactobacillus reuteri/metabolismo
Probióticos
Propano/metabolismo
[Mh] Termos MeSH secundário: Antibacterianos/farmacologia
Antibacterianos/uso terapêutico
Proteínas de Bactérias/genética
Infecções por Clostridium/imunologia
Infecções por Clostridium/terapia
Clostridium difficile/efeitos dos fármacos
Descoberta de Drogas/métodos
Farmacorresistência Bacteriana
Fezes/microbiologia
Fermentação
Microbioma Gastrointestinal
Gliceraldeído/metabolismo
Gliceraldeído/farmacologia
Gliceraldeído/uso terapêutico
Glicerol/imunologia
Glicerol/metabolismo
Seres Humanos
Metabolômica
Propano/farmacologia
Propano/uso terapêutico
Vancomicina/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Bacterial Proteins); 2134-29-4 (3-hydroxypropionaldehyde); 367-47-5 (Glyceraldehyde); 6Q205EH1VU (Vancomycin); PDC6A3C0OX (Glycerol); T75W9911L6 (Propane)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171017
[Lr] Data última revisão:
171017
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170802
[St] Status:MEDLINE


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[PMID]:28699078
[Au] Autor:Auiyawong B; Narawongsanont R; Tantitadapitak C
[Ad] Endereço:Department of Biochemistry, Faculty of Science, Kasetsart University, Bangkok, 10900, Thailand.
[Ti] Título:Characterization of AKR4C15, a Novel Member of Aldo-Keto Reductase, in Comparison with Other Rice AKR(s).
[So] Source:Protein J;36(4):257-269, 2017 Aug.
[Is] ISSN:1875-8355
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Environmental stresses often cause a rapid and excessive accumulation of reactive oxygen species (ROS), the toxicity of which is further amplified by downstream aldehyde production. Aldo-keto reductase (AKR) is a group of enzymes metabolizing aldehyde/ketone to the corresponding alcohol using NADPH as the cofactor. In this study, OsI_20197 (AKR4C15), a novel member of AKR4 subfamily C, was isolated and biochemically characterized. Kinetic studies on bacterially-expressed recombinant AKR4C15 revealed that the enzyme was capable of metabolizing a wide variety of aldehydes but clearly exhibited a preference for three carbon compounds, i.e. methylglyoxal, malondialdehyde and glyceraldehyde. In comparison with His-tagged proteins of AKR4C9 from Arabidopsis and several other rice AKR(s): OsI_04426, OsI_04428, OsI_04429, and OsI_15387, AKR4C15 was the one capable of most efficiently metabolizing MDA and had the highest value of catalytic efficiency, which was higher than the value of AKR4C9, approximately, by 30-fold; while its capability of metabolizing MG was on par with AKR4C9, OsI_04426 and OsI_04428 (AKR4C14); and was considerably higher than the activity of OsI_04429 and OsI_15387. In vivo research on transgenic Arabidopsis seedlings ectopically-expressing AKR4C15 showed that the levels of both MDA and MG were also significantly lower than the levels in wild-type seedlings under both normal and stress conditions, emphasizing the role of AKR4C15 in MG and MDA metabolism. In conclusion, AKR4C15, together with OsI_04426 and AKR4C14, may play protective roles against small reactive aldehydes and medium-chain aldehydes.
[Mh] Termos MeSH primário: Aldeído Redutase/metabolismo
Arabidopsis/enzimologia
Oryza/enzimologia
Proteínas de Plantas/metabolismo
Plântulas/enzimologia
[Mh] Termos MeSH secundário: Aldeído Redutase/genética
Aldo-Ceto Redutases
Sequência de Aminoácidos
Arabidopsis/efeitos dos fármacos
Arabidopsis/genética
Clonagem Molecular
Coenzimas/metabolismo
Escherichia coli/genética
Escherichia coli/metabolismo
Expressão Gênica
Gliceraldeído/metabolismo
Gliceraldeído/farmacologia
Isoenzimas/genética
Isoenzimas/metabolismo
Cinética
Malondialdeído/metabolismo
Malondialdeído/farmacologia
NADP/metabolismo
Oryza/classificação
Oryza/efeitos dos fármacos
Oryza/genética
Estresse Oxidativo
Paraquat/farmacologia
Filogenia
Proteínas de Plantas/genética
Plantas Geneticamente Modificadas
Aldeído Pirúvico/metabolismo
Aldeído Pirúvico/farmacologia
Proteínas Recombinantes/genética
Proteínas Recombinantes/metabolismo
Plântulas/efeitos dos fármacos
Plântulas/genética
Alinhamento de Sequência
Homologia de Sequência de Aminoácidos
Especificidade por Substrato
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Coenzymes); 0 (Isoenzymes); 0 (Plant Proteins); 0 (Recombinant Proteins); 367-47-5 (Glyceraldehyde); 4Y8F71G49Q (Malondialdehyde); 53-59-8 (NADP); 722KLD7415 (Pyruvaldehyde); EC 1.1.1.- (Aldo-Keto Reductases); EC 1.1.1.21 (Aldehyde Reductase); PLG39H7695 (Paraquat)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170713
[St] Status:MEDLINE
[do] DOI:10.1007/s10930-017-9732-z


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[PMID]:28109289
[Au] Autor:Son WR; Nam MH; Hong CO; Kim Y; Lee KW
[Ad] Endereço:Department of Biotechnology, College of Life Science and Biotechnology, Korea University, Seoul, 02841, South Korea.
[Ti] Título:Plantamajoside from Plantago asiatica modulates human umbilical vein endothelial cell dysfunction by glyceraldehyde-induced AGEs via MAPK/NF-κB.
[So] Source:BMC Complement Altern Med;17(1):66, 2017 Jan 21.
[Is] ISSN:1472-6882
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Plantago asiatica has been traditionally used for traditional medicine around East Asia. Plantamajoside (PM), which is isolated from this plant, is known for biological properties including anti-inflammation and antioxidant activity. To demonstrate the biological activity of PM against endothelial dysfunction induced by advanced glycation end-products (AGEs), a cellular inflammatory mechanism system was evaluated in human umbilical vein endothelial cells (HUVECs). METHODS: We obtained PM through previous research in our laboratory. We formed the AGEs from bovine serum albumin with glyceraldehyde in the dark for seven days. To confirm the modulation of the inflammatory mechanism in endothelial dysfunction, we quantified the various pro-inflammatory cytokines and endothelial dysfunction-related proteins in the HUVECs with Western blotting and with real-time and quantitative real-time polymerase chain reactions. RESULTS: Co-treatment with PM and AGEs significantly suppressed inflammatory cytokines and adhesion molecule expression. Moreover, the PM treatment for down-regulated inflammatory signals and blocked monocyte adhesion on the HUVECs. CONCLUSIONS: Theses results demonstrated that PM, as a potential natural compound, protects AGE-induced endothelial cells against inflammatory cellular dysfunction.
[Mh] Termos MeSH primário: Catecóis/farmacologia
Glucosídeos/farmacologia
Produtos Finais de Glicação Avançada/metabolismo
Células Endoteliais da Veia Umbilical Humana/efeitos dos fármacos
Quinases de Proteína Quinase Ativadas por Mitógeno/metabolismo
NF-kappa B/metabolismo
Preparações de Plantas/uso terapêutico
Plantago/química
[Mh] Termos MeSH secundário: Animais
Catecóis/toxicidade
Bovinos
Moléculas de Adesão Celular/metabolismo
Citocinas/metabolismo
Depuradores de Radicais Livres/farmacologia
Glucosídeos/toxicidade
Gliceraldeído/farmacologia
Células Endoteliais da Veia Umbilical Humana/metabolismo
Seres Humanos
Monócitos/efeitos dos fármacos
Monócitos/metabolismo
Transdução de Sinais/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Catechols); 0 (Cell Adhesion Molecules); 0 (Cytokines); 0 (Free Radical Scavengers); 0 (Glucosides); 0 (Glycation End Products, Advanced); 0 (NF-kappa B); 0 (Plant Preparations); 104777-68-6 (plantamajoside); 367-47-5 (Glyceraldehyde); EC 2.7.12.2 (Mitogen-Activated Protein Kinase Kinases)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170123
[St] Status:MEDLINE
[do] DOI:10.1186/s12906-017-1570-1


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[PMID]:28052817
[Au] Autor:Park ES; Park S; Shin JS
[Ad] Endereço:Department of Biotechnology, Yonsei University, Seoul 120-749, South Korea.
[Ti] Título:Spectrophotometric assay for sensitive detection of glycerol dehydratase activity using aldehyde dehydrogenase.
[So] Source:J Biosci Bioeng;123(4):528-533, 2017 Apr.
[Is] ISSN:1347-4421
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:Glycerol dehydratase (GDHt) is a pivotal enzyme for fermentative utilization of glycerol by catalyzing radical-mediated conversion of glycerol into 3-hydroxypropionaldehyde (3-HPA). Precise and sensitive monitoring of cellular GDHt activity during the fermentation process is a prerequisite for reliable metabolic analysis to afford efficient cellular engineering and process optimization. Here we report a new spectrophotometric assay for the sensitive measurement of the GDHt activity with a sub-nanomolar limit of detection (LOD). The assay method employs aldehyde dehydrogenase (ALDH) as a reporter enzyme, so the readout of the GDHt activity is recorded at 340 nm as an increase in UV absorbance which results from NADH generation accompanied by oxidation of 3-HPA to 3-hydroxypropionic acid (3-HP). The GDHt assay was performed under the reaction conditions where the ALDH activity overwhelms the GDHt activity (i.e., 50-fold higher activity of ALDH relative to GDHt activity), affording sensitive detection of GDHt with 360 pM LOD. The ALDH-coupled assay was used to determine kinetic parameters of GDHt for glycerol, leading to K = 0.73 ± 0.09 mM and k = 400 ± 20 s which are in reasonable agreements with the previous reports. Our assay method allowed measurement of even a 10 -fold decrease in the cellular GDHt activity during fermentative production of 3-HP, which demonstrates the detection sensitivity much higher than the previous methods.
[Mh] Termos MeSH primário: Aldeído Desidrogenase/metabolismo
Hidroliases/análise
Hidroliases/metabolismo
Espectrofotometria/métodos
[Mh] Termos MeSH secundário: Fermentação
Gliceraldeído/análogos & derivados
Gliceraldeído/metabolismo
Glicerol/metabolismo
Cinética
Ácido Láctico/análogos & derivados
Ácido Láctico/metabolismo
Limite de Detecção
NAD/metabolismo
Propano/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0U46U6E8UK (NAD); 2134-29-4 (3-hydroxypropionaldehyde); 33X04XA5AT (Lactic Acid); 367-47-5 (Glyceraldehyde); C4ZF6XLD2X (hydracrylic acid); EC 1.2.1.3 (Aldehyde Dehydrogenase); EC 4.2.1.- (Hydro-Lyases); EC 4.2.1.30 (glycerol dehydratase); PDC6A3C0OX (Glycerol); T75W9911L6 (Propane)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170502
[Lr] Data última revisão:
170502
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170106
[St] Status:MEDLINE


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[PMID]:27829124
[Au] Autor:Zaushitsyna O; Dishisha T; Hatti-Kaul R; Mattiasson B
[Ad] Endereço:Biotechnology, Department of Chemistry, Center for Chemistry and Chemical Engineering, Lund University, Box 124, SE-221 00 Lund, Sweden.
[Ti] Título:Crosslinked, cryostructured Lactobacillus reuteri monoliths for production of 3-hydroxypropionaldehyde, 3-hydroxypropionic acid and 1,3-propanediol from glycerol.
[So] Source:J Biotechnol;241:22-32, 2017 Jan 10.
[Is] ISSN:1873-4863
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Crosslinked, cryostructured monoliths prepared from Lactobacillus reuteri cells were evaluated as potential immobilized whole-cell biocatalyst for conversion of glycerol, to potentially important chemicals for the biobased industry, i.e. 3-hydroxypropionaldehyde (3HPA), 3-hydroxypropionic acid (3HP) and 1,3-propanediol (1,3PDO). Glutaraldehyde, oxidized dextran and activated polyethyleneimine/modified polyvinyl alcohol (PEI/PVA) were evaluated as crosslinkers; the latter gave highly stable preparations with maintained viability and biocatalytic activity. Scanning electron microscopy of the PEI/PVA monoliths showed high density of crosslinked cells with wide channels allowing liquid flow through. Flux analysis of the propanediol-utilization pathway, incorporating glycerol/diol dehydratase, propionaldehyde dehydrogenase, 1,3PDO oxidoreductase, phosphotransacylase, and propionate kinase, for conversion of glycerol to the three chemicals showed that the maximum specific reaction rates were -562.6, 281.4, 62.4 and 50.5mg/g h for glycerol consumption, and 3HPA (extracellular), 3HP and 1,3PDO production, respectively. Under optimal conditions using monolith operated as continuous plug flow reactor, 19.7g/L 3HPA was produced as complex with carbohydrazide at a rate of 9.1g/Lh and a yield of 77mol%. Using fed-batch operation, 1,3PDO and 3HP were co-produced in equimolar amounts with a yield of 91mol%. The monoliths embedded in plastic carriers showed high mechanical stability under different modes in a miniaturized plug flow reactor.
[Mh] Termos MeSH primário: Células Imobilizadas/metabolismo
Gliceraldeído/análogos & derivados
Glicerol/metabolismo
Ácido Láctico/análogos & derivados
Lactobacillus reuteri/metabolismo
Propano/metabolismo
Propilenoglicóis/metabolismo
[Mh] Termos MeSH secundário: Células Imobilizadas/citologia
Criogéis/química
Gliceraldeído/análise
Gliceraldeído/metabolismo
Ácido Láctico/análise
Ácido Láctico/metabolismo
Lactobacillus reuteri/citologia
Propano/análise
Propilenoglicóis/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cryogels); 0 (Propylene Glycols); 2134-29-4 (3-hydroxypropionaldehyde); 33X04XA5AT (Lactic Acid); 367-47-5 (Glyceraldehyde); 5965N8W85T (1,3-propanediol); C4ZF6XLD2X (hydracrylic acid); PDC6A3C0OX (Glycerol); T75W9911L6 (Propane)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170228
[Lr] Data última revisão:
170228
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161110
[St] Status:MEDLINE


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[PMID]:27998065
[Au] Autor:Yang J; Zhu Y; Men Y; Sun S; Zeng Y; Zhang Y; Sun Y; Ma Y
[Ad] Endereço:National Engineering Laboratory for Industrial Enzymes, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences , Tianjin 300308, China.
[Ti] Título:Pathway Construction in Corynebacterium glutamicum and Strain Engineering To Produce Rare Sugars from Glycerol.
[So] Source:J Agric Food Chem;64(50):9497-9505, 2016 Dec 21.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Rare sugars are valuable natural products widely used in pharmaceutical and food industries. In this study, we expected to synthesize rare ketoses from abundant glycerol using dihydroxyacetone phosphate (DHAP)-dependent aldolases. First, a new glycerol assimilation pathway was constructed to synthesize DHAP. The enzymes which convert glycerol to 3-hydroxypropionaldehyde and l-glyceraldehyde were selected, and their corresponding aldehyde synthesis pathways were constructed in vivo. Four aldol pathways based on different aldolases and phosphorylase were gathered. Next, three pathways were assembled and the resulting strains synthesized 5-deoxypsicose, 5-deoxysorbose, and 5-deoxyfructose from glucose and glycerol and produce l-fructose, l-tagatose, l-sorbose, and l-psicose with glycerol as the only carbon source. To achieve higher product titer and yield, the recombinant strains were further engineered and fermentation conditions were optimized. Fed-batch culture of engineered strains obtained 38.1 g/L 5-deoxypsicose with a yield of 0.91 ± 0.04 mol product per mol of glycerol and synthesized 20.8 g/L l-fructose, 10.3 g/L l-tagatose, 1.2 g/L l-sorbose, and 0.95 g/L l-psicose.
[Mh] Termos MeSH primário: Corynebacterium glutamicum/metabolismo
Glicerol/metabolismo
Cetoses/biossíntese
Engenharia Metabólica
[Mh] Termos MeSH secundário: Aldeído Liases/metabolismo
Técnicas de Cultura Celular por Lotes
Biomassa
Vias Biossintéticas
Cromatografia Líquida de Alta Pressão
Corynebacterium glutamicum/genética
Escherichia coli/genética
Fermentação
Frutose/biossíntese
Glucose/metabolismo
Gliceraldeído/análogos & derivados
Gliceraldeído/metabolismo
Hexoses/biossíntese
Hidroliases/metabolismo
Espectroscopia de Ressonância Magnética
Propano/metabolismo
Sorbose/biossíntese
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Hexoses); 0 (Ketoses); 2134-29-4 (3-hydroxypropionaldehyde); 23140-52-5 (psicose); 30237-26-4 (Fructose); 367-47-5 (Glyceraldehyde); EC 4.1.2.- (Aldehyde-Lyases); EC 4.2.1.- (Hydro-Lyases); EC 4.2.1.30 (glycerol dehydratase); IY9XDZ35W2 (Glucose); NV2001607Y (Sorbose); PDC6A3C0OX (Glycerol); T75W9911L6 (Propane); T7A20Y888Y (tagatose)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170417
[Lr] Data última revisão:
170417
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161222
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jafc.6b03423


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[PMID]:27671251
[Au] Autor:Wu X; Xu L; Yan M
[Ad] Endereço:a College of Biotechnology and Pharmaceutical Engineering , Nanjing Tech University , Nanjing , P.R. China.
[Ti] Título:A new NAD -dependent glyceraldehyde dehydrogenase obtained by rational design of l-lactaldehyde dehydrogenase from Escherichia coli.
[So] Source:Biosci Biotechnol Biochem;80(12):2306-2310, 2016 Dec.
[Is] ISSN:1347-6947
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:NAD + -dependent glyceraldehyde dehydrogenases usually had lower activity in the nonphosphorylated Entner-Doudoroff (nED) pathway. In the present study, a new NAD + -dependent glyceraldehyde dehydrogenase was engineered from l-lactaldehyde dehydrogenase of E. coli (EC: 1.2.1.22). Through comparison of the sequence alignment and the active center model, we found that a residue N286 of l-lactaldehyde dehydrogenase contributed an important structure role to substrate identification. By free energy calculation, three mutations (N286E, N286H, N286T) were chosen to investigate the change of substrate specificity of the enzyme. All mutants were able to oxidate glyceraldehyde. Especially, N286T showed the highest activity of 1.1U/mg, which was 5-fold higher than the reported NAD + -dependent glyceraldehyde dehydrogenases, and 70% activity was retained at 55 °C after an hour. Compared to l-lactaldehyde, N286T had a one-third lower K value to glyceraldehyde.
[Mh] Termos MeSH primário: Aldeído Oxirredutases/genética
Aldeído Oxirredutases/metabolismo
Escherichia coli/enzimologia
Gliceraldeído/metabolismo
Mutagênese Sítio-Dirigida
NAD/metabolismo
[Mh] Termos MeSH secundário: Aldeído Oxirredutases/química
Domínio Catalítico
Estabilidade Enzimática
Escherichia coli/genética
Concentração de Íons de Hidrogênio
Modelos Moleculares
Mutação
Alinhamento de Sequência
Temperatura Ambiente
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0U46U6E8UK (NAD); 367-47-5 (Glyceraldehyde); EC 1.2.- (Aldehyde Oxidoreductases); EC 1.2.1.22 (lactaldehyde dehydrogenase)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170130
[Lr] Data última revisão:
170130
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160928
[St] Status:MEDLINE


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[PMID]:27495826
[Au] Autor:Fernández-Cruz ML; Martín-Cabrejas I; Pérez-Del Palacio J; Gaya P; Díaz-Navarro C; Navas JM; Medina M; Arqués JL
[Ad] Endereço:Departamento de Medio Ambiente, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Carretera de la Coruña Km 7, Madrid, Spain. Electronic address: fcruz@inia.es.
[Ti] Título:In vitro toxicity of reuterin, a potential food biopreservative.
[So] Source:Food Chem Toxicol;96:155-9, 2016 Oct.
[Is] ISSN:1873-6351
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Reuterin has a high potential as a food preservative due to both its chemical characteristics and its antimicrobial activity against food-borne pathogens and spoilage bacteria. However, there is a lack of information about its toxicity and its capacity to interfere with the metabolism of drugs by inhibiting cytochrome P450 (CYP) activity. The results of this study indicated that reuterin exhibited a moderate cytotoxicity in the human hepatoma cell line HepG2 according to assays measuring three different endpoints in the same set of cells. Reuterin was much less toxic than acrolein and only four times more toxic than diacetyl, a generally recognized as safe flavoring compound. In vitro experiments utilizing human liver microsomes showed that reuterin presents low possibility of displaying in vivo drug interactions by inhibition of CYP3A4, CYP2D6, and CYP2C9. Therefore, reuterin can be considered a promising food biopreservative, although additional toxicology research is needed before permission for use can be granted.
[Mh] Termos MeSH primário: Sobrevivência Celular/efeitos dos fármacos
Inibidores das Enzimas do Citocromo P-450/toxicidade
Conservantes de Alimentos
Gliceraldeído/análogos & derivados
Propano/toxicidade
[Mh] Termos MeSH secundário: Citocromo P-450 CYP2C9/química
Citocromo P-450 CYP2C9/metabolismo
Citocromo P-450 CYP2D6/química
Citocromo P-450 CYP2D6/metabolismo
Citocromo P-450 CYP3A/química
Citocromo P-450 CYP3A/metabolismo
Interações Medicamentosas
Gliceraldeído/toxicidade
Células Hep G2
Seres Humanos
Técnicas In Vitro
Microssomos Hepáticos/efeitos dos fármacos
Microssomos Hepáticos/enzimologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cytochrome P-450 Enzyme Inhibitors); 0 (Food Preservatives); 2134-29-4 (3-hydroxypropionaldehyde); 367-47-5 (Glyceraldehyde); EC 1.14.13.- (CYP2C9 protein, human); EC 1.14.13.- (Cytochrome P-450 CYP2C9); EC 1.14.13.67 (CYP3A4 protein, human); EC 1.14.14.1 (Cytochrome P-450 CYP2D6); EC 1.14.14.1 (Cytochrome P-450 CYP3A); T75W9911L6 (Propane)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170315
[Lr] Data última revisão:
170315
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160807
[St] Status:MEDLINE


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[PMID]:27289193
[Au] Autor:Gómez-Torres N; Ávila M; Delgado D; Garde S
[Ad] Endereço:Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Departamento de Tecnología de Alimentos, Carretera de La Coruña km 7, 28040 Madrid, Spain.
[Ti] Título:Effect of reuterin-producing Lactobacillus reuteri coupled with glycerol on the volatile fraction, odour and aroma of semi-hard ewe milk cheese.
[So] Source:Int J Food Microbiol;232:103-10, 2016 Sep 02.
[Is] ISSN:1879-3460
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The effect of the biopreservation system formed by Lactobacillus reuteri INIA P572, a reuterin-producing strain, and glycerol (required for reuterin production), on the volatile fraction, aroma and odour of industrial sized semi-hard ewe milk cheese (Castellano type) was investigated over a 3-month ripening period. The volatile compounds were extracted and analyzed by SPME-GC-MS and cheese odour and aroma profiles were studied by descriptive sensory analysis. Control cheese was made only with a mesophilic starter and experimental cheeses with L. reuteri were made with and without glycerol. The addition of L. reuteri INIA P572 to milk enhanced the formation of six volatile compounds. Despite the changes in the volatile compounds profile, the use of L. reuteri INIA P572 did not noticeably affect the sensory characteristics of cheese. On the other hand, the addition of L. reuteri INIA P572 coupled with 30mM glycerol enhanced the formation of twelve volatile compounds, but decreased the formation of five ones. The use of the biopreservation system did not affect overall odour and aroma quality of cheese although it resulted in a significant decrease of the odour intensity scores. In addition, this cheese received significant higher scores for "cheesy" aroma and significant lower scores for the aroma attributes "milky", "caramel" and "yogurt-like". The first two axes of a principal component analysis (PCA) performed for selected volatile compounds and sensory characteristics, accounting for 75% of the variability between cheeses, separated cheeses made with L. reuteri INIA P572 and glycerol from the rest of cheeses, and also differentiated control cheese from cheeses made with L. reuteri INIA P572 from day 60 onward. Our results showed that the reuterin-producing L. reuteri INIA P572 strain, when coupled with glycerol, may be a suitable biopreservation system to use in cheese without affecting odour and aroma quality.
[Mh] Termos MeSH primário: Queijo/análise
Conservação de Alimentos/métodos
Gliceraldeído/análogos & derivados
Glicerol/metabolismo
Lactobacillus reuteri/metabolismo
Odorantes/análise
Propano/metabolismo
Compostos Orgânicos Voláteis/metabolismo
[Mh] Termos MeSH secundário: Animais
Cromatografia Gasosa-Espectrometria de Massas
Gliceraldeído/metabolismo
Seres Humanos
Leite/química
Ovinos
Paladar
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Volatile Organic Compounds); 2134-29-4 (3-hydroxypropionaldehyde); 367-47-5 (Glyceraldehyde); PDC6A3C0OX (Glycerol); T75W9911L6 (Propane)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160613
[St] Status:MEDLINE


  10 / 932 MEDLINE  
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[PMID]:26938861
[Au] Autor:Martins-Pinheiro M; Lima WC; Asif H; Oller CA; Menck CF
[Ad] Endereço:Dept of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, 05508-900, Brazil.
[Ti] Título:Evolutionary and Functional Relationships of the dha Regulon by Genomic Context Analysis.
[So] Source:PLoS One;11(3):e0150772, 2016.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:3-hydroxypropionaldehyde (3-HPA) and 1,3-propanediol (1,3-PD) are subproducts of glycerol degradation and of economical interest as they are used for polymers synthesis, such as polyesters and polyurethanes. Some few characterized bacterial species (mostly from Firmicutes and Gamma-proteobacteria groups) are able to catabolize these monomers from glycerol using the gene products from the dha regulon. To expand our knowledge and direct further experimental studies on the regulon and related genes for the anaerobic glycerol metabolism, an extensive genomic screening was performed to identify the presence of the dha genes in fully sequenced prokaryotic genomes. Interestingly, this work shows that although only few bacteria species are known to produce 3-HPA or 1,3-PD, the incomplete regulon is found in more than 100 prokaryotic genomes. However, the complete pathway is found only in a few dozen species belonging to five different taxonomic groups, including one Archaea species, Halalkalicoccus jeotgali. Phylogenetic analysis and conservation of both gene synteny and primary sequence similarity reinforce the idea that these genes have a common origin and were possibly acquired by lateral gene transfer (LGT). Besides the evolutionary aspect, the identification of homologs from several different organisms may predict potential alternative targets for faster or more efficient biological synthesis of 3-HPA or 1,3-PD.
[Mh] Termos MeSH primário: Archaea/genética
Bactérias/genética
Evolução Molecular
Gliceraldeído/análogos & derivados
Propano/química
Propilenoglicóis/química
Regulon
[Mh] Termos MeSH secundário: Aerobiose
Algoritmos
Sequência de Aminoácidos
Fermentação
Transferência Genética Horizontal
Genoma Arqueal
Genoma Bacteriano
Genômica
Gliceraldeído/química
Glicerol/química
Glicerol/metabolismo
Funções Verossimilhança
Dados de Sequência Molecular
Filogenia
Homologia de Sequência de Aminoácidos
Especificidade da Espécie
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Propylene Glycols); 2134-29-4 (3-hydroxypropionaldehyde); 367-47-5 (Glyceraldehyde); 5965N8W85T (1,3-propanediol); PDC6A3C0OX (Glycerol); T75W9911L6 (Propane)
[Em] Mês de entrada:1608
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160304
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0150772



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