Base de dados : MEDLINE
Pesquisa : D02.047.644 [Categoria DeCS]
Referências encontradas : 826 [refinar]
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[PMID]:28880548
[Au] Autor:Klaus A; Pfirrmann T; Glomb MA
[Ad] Endereço:Institute of Chemistry, Food Chemistry, Martin-Luther-University Halle-Wittenberg , Kurt-Mothes-Str. 2, 06120 Halle/Saale, Germany.
[Ti] Título:Transketolase A from E. coli Significantly Suppresses Protein Glycation by Glycolaldehyde and Glyoxal in Vitro.
[So] Source:J Agric Food Chem;65(37):8196-8202, 2017 Sep 20.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Short-chained carbonyl species such as glycolaldehyde and its oxidized pendant glyoxal are highly reactive Maillard agents, leading to the formation of protein modifications. These advanced glycation endproducts have gained considerable interest as they have been linked to various pathologies in vivo. The ability of transketolase to use glycolaldehyde as a substrate suggested the possibility to modulate carbonyl-driven Maillard reactions. Model incubations with recombinant transketolase A from Escherichia coli in the presence of bovine serum albumin and glycolaldehyde indeed led to a decrease in glycolaldehyde concentrations paralleled by the enzymatic conversion to erythrulose. As a result, reversibly protein-bound glycolaldehyde and the major final endproduct N -carboxymethyl lysine were significantly reduced by approximately 50%, respectively. Glycolaldehyde is easily oxidized to glyoxal in the presence of amines and oxygen. In the presence of transketolase, the lower amounts of glycolaldehyde therefore also strongly suppressed the formation of glyoxal specific arginine modifications, measured as 5-(2-imino-5-oxo-1-imidazolidinyl)norvaline after acid hydrolysis.
[Mh] Termos MeSH primário: Acetaldeído/análogos & derivados
Proteínas de Escherichia coli/química
Escherichia coli/enzimologia
Glioxal/química
Proteínas/química
Transcetolase/química
[Mh] Termos MeSH secundário: Acetaldeído/química
Produtos Finais de Glicação Avançada/química
Cinética
Oxirredução
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Escherichia coli Proteins); 0 (Glycation End Products, Advanced); 0 (Proteins); 50NP6JJ975 (Glyoxal); EC 2.2.1.1 (Transketolase); GO1N1ZPR3B (Acetaldehyde); W0A0XPU08U (glycolaldehyde)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170908
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jafc.7b03183


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[PMID]:28796828
[Au] Autor:Bussolati G; Annaratone L; Berrino E; Miglio U; Panero M; Cupo M; Gugliotta P; Venesio T; Sapino A; Marchiò C
[Ad] Endereço:Department of Medical Sciences, University of Turin, Turin, Italy.
[Ti] Título:Acid-free glyoxal as a substitute of formalin for structural and molecular preservation in tissue samples.
[So] Source:PLoS One;12(8):e0182965, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Tissue fixation in phosphate buffered formalin (PBF) remains the standard procedure in histopathology, since it results in an optimal structural, antigenic and molecular preservation that justifies the pivotal role presently played by diagnoses on PBF-fixed tissues in precision medicine. However, toxicity of formaldehyde causes an environmental concern and may demand substitution of this reagent. Having observed that the reported drawbacks of commercially available glyoxal substitutes of PBF (Prefer, Glyo-fix, Histo-Fix, Histo-CHOICE, and Safe-Fix II) are likely related to their acidity, we have devised a neutral fixative, obtained by removing acids from the dialdehyde glyoxal with an ion-exchange resin. The resulting glyoxal acid-free (GAF) fixative has been tested in a cohort of 30 specimens including colon (N = 25) and stomach (N = 5) cancers. Our results show that GAF fixation produces a tissue and cellular preservation similar to that produced by PBF. Comparable immuno-histochemical and molecular (DNA and RNA) analytical data were obtained. We observed a significant enrichment of longer DNA fragment size in GAF-fixed compared to PBF-fixed samples. Adoption of GAF as a non-toxic histological fixative of choice would require a process of validation, but the present data suggest that it represents a reliable candidate.
[Mh] Termos MeSH primário: Fixadores/química
Formaldeído/química
Glioxal/química
Fixação de Tecidos/métodos
[Mh] Termos MeSH secundário: DNA/análise
Seres Humanos
Imuno-Histoquímica/métodos
Hibridização in Situ Fluorescente/métodos
RNA/análise
Análise de Sequência de DNA/métodos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fixatives); 1HG84L3525 (Formaldehyde); 50NP6JJ975 (Glyoxal); 63231-63-0 (RNA); 9007-49-2 (DNA)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171004
[Lr] Data última revisão:
171004
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170811
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0182965


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[PMID]:28653535
[Au] Autor:Zhao D; Li L; Le TT; Larsen LB; Su G; Liang Y; Li B
[Ad] Endereço:College of Food Science and Engineering, South China University of Technology , 381 Wushan Road, Tianhe District, Guangzhou, 510640, China.
[Ti] Título:Digestibility of Glyoxal-Glycated ß-Casein and ß-Lactoglobulin and Distribution of Peptide-Bound Advanced Glycation End Products in Gastrointestinal Digests.
[So] Source:J Agric Food Chem;65(28):5778-5788, 2017 Jul 19.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:This work reports the influence of glyoxal (GO)-derived glycation on the gastrointestinal enzymatic hydrolysis of ß-lactoglobulin and ß-casein. Reduced digestibility of glycated proteins was found in both gastric and intestinal stage. Distribution of Maillard reaction products in digests with different molecular weight ranges was investigated subsequently. The colorless and brown MRPs largely presented in the digests smaller than 20 kDa. However, the resistance of fluorescent advanced glycation end products (AGEs) to enzymatic hydrolysis gradually increased during glycation, rendering fluorescent AGEs largely present in the digests larger than 20 kDa. No free N (ε)-carboxymethyllysine (CML) was detected in digests. The relative amount of CML in digests larger than 1 kDa was higher than that of Lys, demonstrating the hindrance of CML to enzymatic hydrolysis. This study highlights the resistance of GO-derived AGEs to digestive proteases via blockage of tryptic cleavage sites or steric hindrance, which is a barrier to the absorption of dietary AGEs.
[Mh] Termos MeSH primário: Caseínas/metabolismo
Trato Gastrointestinal/metabolismo
Produtos Finais de Glicação Avançada/metabolismo
Glioxal/metabolismo
Lactoglobulinas/metabolismo
[Mh] Termos MeSH secundário: Caseínas/química
Digestão
Glicosilação
Glioxal/química
Lactoglobulinas/química
Modelos Biológicos
Peptídeos/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Caseins); 0 (Glycation End Products, Advanced); 0 (Lactoglobulins); 0 (Peptides); 50NP6JJ975 (Glyoxal)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170628
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jafc.7b01951


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[PMID]:28641833
[Au] Autor:Pastor-Belda M; Fernández-García AJ; Campillo N; Pérez-Cárceles MD; Motas M; Hernández-Córdoba M; Viñas P
[Ad] Endereço:Department of Analytical Chemistry, Faculty of Chemistry, Regional Campus of International Excellence "Campus Mare Nostrum", University of Murcia, E-30100 Murcia, Spain.
[Ti] Título:Glyoxal and methylglyoxal as urinary markers of diabetes. Determination using a dispersive liquid-liquid microextraction procedure combined with gas chromatography-mass spectrometry.
[So] Source:J Chromatogr A;1509:43-49, 2017 Aug 04.
[Is] ISSN:1873-3778
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Glyoxal (GO) and methylglyoxal (MGO) are α-oxoaldehydes that can be used as urinary diabetes markers. In this study, their levels were measured using a sample preparation procedure based on salting-out assisted liquid-liquid extraction (SALLE) and dispersive liquid-liquid microextraction (DLLME) combined with gas chromatography-mass spectrometry (GC-MS). The effect of the derivatization reaction with 2,3-diaminonaphthalene, the addition of acetonitrile and sodium chloride to urine, and the DLLME step using the acetonitrile extract as dispersant solvent and carbon tetrachloride as extractant solvent were carefully optimized. Quantification was performed by the internal standard method, using 5-bromo-2-chloroanisole. The intraday and interday precisions were lower than 6%. Limits of detection were 0.12 and 0.06ngmL , and enrichment factors 140 and 130 for GO and MGO, respectively. The concentrations of these α-oxoaldehydes in urine were between 0.9 and 35.8ngg levels (creatinine adjusted). A statistical comparison of the analyte contents of urine samples from non-diabetic and diabetic patients pointed to significant differences (P=0.046, 24 subjects investigated), particularly regarding MGO, which was higher in diabetic patients. The novelty of this study compared with previous procedures lies in the treatment of the urine sample by SALLE based on the addition of acetonitrile and sodium chloride to the urine. The DLLME procedure is performed with a sedimented drop of the extractant solvent, without a surfactant reagent, and using acetonitrile as dispersant solvent. Separation of the analytes was performed using GC-MS detection, being the analytes unequivocal identified. The proposed procedure is the first microextraction method applied to the analysis of urine samples from diabetic and non-diabetic patients that allows a clear differentiation between both groups using a simple analysis.
[Mh] Termos MeSH primário: Diabetes Mellitus/urina
Cromatografia Gasosa-Espectrometria de Massas/métodos
Glioxal/isolamento & purificação
Glioxal/urina
Microextração em Fase Líquida/métodos
Aldeído Pirúvico/isolamento & purificação
Aldeído Pirúvico/urina
[Mh] Termos MeSH secundário: Adulto
Feminino
Seres Humanos
Limite de Detecção
Masculino
Meia-Idade
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE
[Nm] Nome de substância:
50NP6JJ975 (Glyoxal); 722KLD7415 (Pyruvaldehyde)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171016
[Lr] Data última revisão:
171016
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170624
[St] Status:MEDLINE


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[PMID]:28623132
[Au] Autor:Jang S; Kwon DM; Kwon K; Park C
[Ad] Endereço:Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 291, Daehak-ro, Yuseong-gu, Daejeon 34141, Republic of Korea.
[Ti] Título:Generation and characterization of mouse knockout for glyoxalase 1.
[So] Source:Biochem Biophys Res Commun;490(2):460-465, 2017 Aug 19.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Glyoxalase 1 (Glo1) is the first enzyme involved in glutathione-dependent detoxification of methylglyoxal, eventually generating d-lactate by the second enzyme glyoxalase 2 (Glo2). An accumulation of intracellular glyoxal and methylglyoxal leads to protein malfunction and mutation via formation of the advanced glycation end products (AGEs). Studies on mouse behavior suggest that methylglyoxal has anxiolytic properties. In this report, we generated and characterized a mouse knockout for Glo1. The knockout mice were viable without a pronounced phenotypic defect. Increased level of AGEs in Glo1 knockout mice was detected by immunoblotting with anti-MGH1 in liver homogenate, but not in brain. Alterations in behavior were observed in open field, light-dark transition, and tail suspension test. Open field data indicate increased exploration for novel environment and entry/stay in center zone in Glo1 knockout mice. In addition, increased light-dark transition and immobility was observed in the knockout mice. These data indicate that Glo1 knockout reduces anxiety-like behavior, but increases depression-like behavior.
[Mh] Termos MeSH primário: Ansiedade/genética
Depressão/genética
Lactoilglutationa Liase/genética
[Mh] Termos MeSH secundário: Animais
Ansiedade/metabolismo
Depressão/metabolismo
Deleção de Genes
Glutationa/metabolismo
Produtos Finais de Glicação Avançada/metabolismo
Glioxal/metabolismo
Lactoilglutationa Liase/metabolismo
Fígado/metabolismo
Masculino
Camundongos
Camundongos Endogâmicos C57BL
Camundongos Knockout
Aldeído Pirúvico/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Glycation End Products, Advanced); 50NP6JJ975 (Glyoxal); 722KLD7415 (Pyruvaldehyde); EC 4.4.1.5 (Lactoylglutathione Lyase); GAN16C9B8O (Glutathione)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170618
[St] Status:MEDLINE


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[PMID]:28335565
[Au] Autor:Park CB; Song MJ; Choi NW; Kim S; Jeon HP; Kim S; Kim Y
[Ad] Endereço:Environmental Safety Group, Korea Institute of Science and Technology (KIST) Europe, 66123 Saarbrücken, Germany. cb.park@kist-europe.de.
[Ti] Título:An Eco-Safety Assessment of Glyoxal-Containing Cellulose Ether on Freeze-Dried Microbial Strain, Cyanobacteria, Daphnia, and Zebrafish.
[So] Source:Int J Environ Res Public Health;14(3), 2017 Mar 21.
[Is] ISSN:1660-4601
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:The objective of this study was to investigate the aquatic-toxic effects of glyoxal-containing cellulose ether with four different glyoxal concentrations (0%, 1.4%, 2.3%, and 6.3%) in response to global chemical regulations, e.g., European Union Classification, Labeling and Packaging (EU CLP). Toxicity tests of glyoxal-containing cellulose ether on 11 different microbial strains, , , and zebrafish embryos were designed as an initial stage of toxicity screening and performed in accordance with standardized toxicity test guidelines. Glyoxal-containing cellulose ether showed no significant toxic effects in the toxicity tests of the 11 freeze-dried microbial strains, , and zebrafish embryos. Alternatively, 6.3% glyoxal-containing cellulose ether led to a more than 60% reduction in growth after 7 days of exposure. Approximately 10% of the developmental abnormalities (e.g., bent spine) in zebrafish embryos were also observed in the group exposed to 6.3% glyoxal-containing cellulose ether after 6 days of exposure. These results show that 6.3% less glyoxal-containing cellulose ether has no acute toxic effects on aquatic organisms. However, 6.3% less glyoxal-containing cellulose ether may affect the health of aquatic organisms with long-term exposure. In order to better evaluate the eco-safety of cellulosic products containing glyoxal, further studies regarding the toxic effects of glyoxal-containing cellulose ether with long-term exposure are required. The results from this study allow us to evaluate the aquatic-toxic effects of glyoxal-containing cellulosic products, under EU chemical regulations, on the health of aquatic organisms.
[Mh] Termos MeSH primário: Celulose
Éteres
Glioxal/toxicidade
Poluentes Químicos da Água/toxicidade
[Mh] Termos MeSH secundário: Animais
Organismos Aquáticos/efeitos dos fármacos
Daphnia/efeitos dos fármacos
Relação Dose-Resposta a Droga
Glioxal/administração & dosagem
Testes de Toxicidade
Poluentes Químicos da Água/administração & dosagem
Peixe-Zebra/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Ethers); 0 (Water Pollutants, Chemical); 50NP6JJ975 (Glyoxal); 9004-34-6 (Cellulose)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170717
[Lr] Data última revisão:
170717
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170325
[St] Status:MEDLINE


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[PMID]:28233503
[Au] Autor:Liu G; Xia Q; Lu Y; Zheng T; Sang S; Lv L
[Ad] Endereço:Department of Food Science and Technology, Nanjing Normal University , 122 Ninghai Road, Nanjing 210097, People's Republic of China.
[Ti] Título:Influence of Quercetin and Its Methylglyoxal Adducts on the Formation of α-Dicarbonyl Compounds in a Lysine/Glucose Model System.
[So] Source:J Agric Food Chem;65(10):2233-2239, 2017 Mar 15.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Increasing evidence has identified α-dicarbonyl compounds, the reactive intermediates generated during Maillard reaction, as the potential factors to cause protein glycation and the development of chronic diseases. Therefore, there is an urgent need to decrease the levels of reactive dicarbonyl compounds in foods. In this study, we investigated the inhibitory effect of quercetin, a major dietary flavonoid, and its major mono- and di-MGO adducts on the formation of dicarbonyl compounds, such as methylglyoxal (MGO) and glyoxal (GO), in a lysine/glucose aqueous system, a model system to reflect the Maillard reaction in food process. Our result indicated that quercetin could efficiently inhibit the formation of MGO and GO in a time-dependent manner. Further mechanistic study was conducted by monitoring the formation of quercetin oxidation and conjugation products using LC-MS/MS. Quercetin MGO adducts, quercetin quinones, and the quinones of quercetin MGO adducts were detected in the system, indicating quercetin plays a dual role in inhibiting the formation of MGO and GO by scavenging free radicals generated in the system and trapping of MGO and GO to form MGO adducts. In addition, we prepared the mono- and di-MGO quercetin adducts and investigated their antioxidant activity and trapping capacity of MGO and GO. Our results indicated that both mono- and di-MGO quercetin adducts could scavenge the DPPH radical in a dose-dependent manner with >40% DPPH scavenged by the MGO adducts at 10 µM, and the di-MGO quercetin adduct could further trap MGO to generate tri-MGO adducts. Therefore, we demonstrate for the first time that quercetin MGO adducts retain the antioxidant activity and trapping capacity of reactive dicarbonyl species.
[Mh] Termos MeSH primário: Glucose/química
Lisina/química
Aldeído Pirúvico/química
Quercetina/química
[Mh] Termos MeSH secundário: Glioxal/química
Temperatura Alta
Reação de Maillard
Estrutura Molecular
Espectrometria de Massas em Tandem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
50NP6JJ975 (Glyoxal); 722KLD7415 (Pyruvaldehyde); 9IKM0I5T1E (Quercetin); IY9XDZ35W2 (Glucose); K3Z4F929H6 (Lysine)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170526
[Lr] Data última revisão:
170526
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170225
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jafc.6b05811


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[PMID]:28217874
[Au] Autor:Liu Y; Li G; Chen J; Liu Z; Liu Z; Jiang J
[Ad] Endereço:Key Laboratory of Applied Surface and Colloid Chemistry, Ministry of Education, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an, Shaanxi Province, 710062, P. R. China.
[Ti] Título:Fabricating Triple-Sensitive Polymer Nano-Aggregates via an Aqueous Iminoboronate Multicomponent Reaction.
[So] Source:Macromol Rapid Commun;38(8), 2017 Apr.
[Is] ISSN:1521-3927
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:A triple-sensitive polymer of poly(ethylene glycol)-iminoboronate nitrobenzyl ethanediol chelate (PEG-INEC) is efficiently fabricated via the convenient aqueous iminoboronate multi-component reaction (MCR) of methoxypolyethylene glycol amine (mPEG-NH ), 2-formylphenylboronic acid (FPBA), and bis(2-nitrophenyl) ethanediol (BNPE, a photo-cleavable nitrobenzyl alcohol derivate). The aqueous MCR synthetic procedure is followed using H NMR and turbidity analysis. It is shown that polymer nano-aggregates of PEG-INEC in aqueous solution can be dissociated through the stimuli responsive reactions of the hydrophobic iminoboronate nitrobenzyl ethanediol chelates (INECs) when exposed to UV light, acid, and H O , respectively. Furthermore, upon the stimulation of combined triggers, the dissociation of polymer nano-aggregates can be accelerated to different extents, resulting in the synergistic release of encapsulated hydrophobic molecules in water. The proposed facile and general method is quite desirable and of great importance in practical applications like drug and gene delivery.
[Mh] Termos MeSH primário: Benzaldeídos/química
Ácidos Borônicos/química
Glioxal/química
Polietilenoglicóis/química
Polímeros/química
[Mh] Termos MeSH secundário: Ácidos/farmacologia
Antibióticos Antineoplásicos/administração & dosagem
Antibióticos Antineoplásicos/química
Antibióticos Antineoplásicos/farmacocinética
Doxorrubicina/administração & dosagem
Doxorrubicina/química
Doxorrubicina/farmacocinética
Portadores de Fármacos/química
Sistemas de Liberação de Medicamentos/métodos
Liberação Controlada de Fármacos/efeitos dos fármacos
Liberação Controlada de Fármacos/efeitos da radiação
Peróxido de Hidrogênio/farmacologia
Interações Hidrofóbicas e Hidrofílicas
Modelos Químicos
Estrutura Molecular
Tamanho da Partícula
Polímeros/síntese química
Espectroscopia de Prótons por Ressonância Magnética
Raios Ultravioleta
Água/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (2-formylphenylboronic acid); 0 (Acids); 0 (Antibiotics, Antineoplastic); 0 (Benzaldehydes); 0 (Boronic Acids); 0 (Drug Carriers); 0 (Polymers); 059QF0KO0R (Water); 30IQX730WE (Polyethylene Glycols); 50NP6JJ975 (Glyoxal); 80168379AG (Doxorubicin); 9004-74-4 (monomethoxypolyethylene glycol); BBX060AN9V (Hydrogen Peroxide)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170601
[Lr] Data última revisão:
170601
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170221
[St] Status:MEDLINE
[do] DOI:10.1002/marc.201600805


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[PMID]:28192135
[Au] Autor:Vilanova B; Fernández D; Casasnovas R; Pomar AM; Alvarez-Idaboy JR; Hernández-Haro N; Grand A; Adrover M; Donoso J; Frau J; Muñoz F; Ortega-Castro J
[Ad] Endereço:Department de Química, Institut Universitari d'Investigació en Ciències de la Salut (IUNICS), Universitat de les Illes Balears, 07122 Palma de Mallorca, Spain; Instituto de Investigación Sanitaria de Palma (IdISPA), 07010 Palma de Mallorca, Spain. Electronic address: bartomeu.vilanova@uib.es.
[Ti] Título:Formation mechanism of glyoxal-DNA adduct, a DNA cross-link precursor.
[So] Source:Int J Biol Macromol;98:664-675, 2017 May.
[Is] ISSN:1879-0003
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:DNA nucleobases undergo non-enzymatic glycation to nucleobase adducts which can play important roles in vivo. In this work, we conducted a comprehensive experimental and theoretical kinetic study of the mechanisms of formation of glyoxal-guanine adducts over a wide pH range in order to elucidate the molecular basis for the glycation process. Also, we performed molecular dynamics simulations to investigate how open or cyclic glyoxal-guanine adducts can cause structural changes in an oligonucleotide model. A thermodynamic study of other glycating agents including methylglyoxal, acrolein, crotonaldehyde, 4-hydroxynonenal and 3-deoxyglucosone revealed that, at neutral pH, cyclic adducts were more stable than open adducts; at basic pH, however, the open adducts of 3-deoxyglucosone, methylglyoxal and glyoxal were more stable than their cyclic counterparts. This result can be ascribed to the ability of the adducts to cross-link DNA. The new insights may contribute to improve our understanding of the connection between glycation and DNA cross-linking.
[Mh] Termos MeSH primário: Adutos de DNA/química
DNA/química
Glioxal/química
Guanina/química
[Mh] Termos MeSH secundário: Aldeídos/química
DNA/genética
Adutos de DNA/genética
Dano ao DNA/genética
Glicosilação
Cinética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Aldehydes); 0 (DNA Adducts); 50NP6JJ975 (Glyoxal); 5Z93L87A1R (Guanine); 9007-49-2 (DNA)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170324
[Lr] Data última revisão:
170324
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170214
[St] Status:MEDLINE


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[PMID]:28183616
[Au] Autor:Kumar A; Kumar A
[Ad] Endereço:Department of Biotechnology, National Institute of Technology, G. E. Road, Raipur 492010, Chhattisgarh, India.
[Ti] Título:Development and characterization of tripolymeric and bipolymeric composite films using glyoxal as a potent crosslinker for biomedical application.
[So] Source:Mater Sci Eng C Mater Biol Appl;73:333-339, 2017 Apr 01.
[Is] ISSN:1873-0191
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:For various biomedical applications, biopolymeric films are often crosslinked using chemical crosslinker such as glutaraldehyde, which is considered as a toxic chemical. In this report, we have prepared and characterized biopolymeric films using different combinations of chitosan, CMC, alginate and PVA using glyoxal as the crosslinker. The prepared films were subjected to various physico-chemical and mechanical characterizations such as swelling index, surface pH, surface morphology analysis using SEM, interact ion study using XRD, flexibility study using tensile testing and hardness testing. Glyoxal crosslinking resulted in variation of physico-chemical and mechanical alteration of the chitosan-PVA films while it had the negligible effect on the CAP film. Further, the hardness of the films demonstrated a decrease in value in the crosslinked films as compared to non-crosslinked films. We have interpreted that glyoxal is a potential crosslinker for chitosan-based composite polymers while in this case, it did not show any significant effect on CMC and alginate based composite structures. Therefore, using this type of films would be the cheap, safe and new alternative in drug delivery and other biomedical applications.
[Mh] Termos MeSH primário: Tecnologia Biomédica/métodos
Reagentes para Ligações Cruzadas/química
Glioxal/química
Polímeros/química
[Mh] Termos MeSH secundário: Dureza
Concentração de Íons de Hidrogênio
Microscopia Eletrônica de Varredura
Solventes/química
Espectrometria por Raios X
Estresse Mecânico
Propriedades de Superfície
Resistência à Tração
Difração de Raios X
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cross-Linking Reagents); 0 (Polymers); 0 (Solvents); 50NP6JJ975 (Glyoxal)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170903
[Lr] Data última revisão:
170903
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170211
[St] Status:MEDLINE



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