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[PMID]:23255614
[Au] Autor:Hagos Y; Burckhardt G; Burckhardt BC
[Ad] Endereço:Department of Systemic Physiology and Pathophysiology, University Medical Center Göttingen, Göttingen, Germany.
[Ti] Título:Human organic anion transporter OAT1 is not responsible for glutathione transport but mediates transport of glutamate derivatives.
[So] Source:Am J Physiol Renal Physiol;304(4):F403-9, 2013 Feb 15.
[Is] ISSN:1522-1466
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Due to their clearance function, the kidneys are exposed to high concentrations of oxidants and potentially toxic substances. To maintain cellular integrity, renal cells have to be protected by sufficient concentrations of the antioxidant glutathione (GSH). We tested whether GSH or its precursors are taken up by human organic anion transporters 1 (OAT1) and 3 (OAT3) stably expressed in HEK293 cells. GSH did not inhibit uptake of p-aminohippurate (PAH) or of estrone sulfate (ES) in OAT3-transfected HEK293 cells. In OAT1-transfected cells, GSH reduced the uptake of PAH marginally. Among the GSH constituent amino acids, glutamate, cysteine, and glycine, only glutamate inhibited OAT1, but labeled glutamate was not taken up by a probenecid-inhibitable transport system. Thus OAT1 binds glutamate but is unable to translocate it. The GSH precursor dipeptide, cysteinyl glycine (cysgly), and the glutamate derivative N-acetyl glutamate (NAG), inhibited uptake of PAH when present in the medium and trans-stimulated uptake of PAH from the intracellular side, indicating that they are hitherto unrecognized transported substrates of OAT1. N-acetyl aspartate weakly interacted with OAT1, but aspartate did not. NAG inhibited also OAT3, albeit with much lower affinity compared with OAT1, and glutamate did not interact with OAT3 at all. Taken together, human OAT3 and OAT1 cannot be involved in renal GSH extraction from the blood. However, OAT1 could support intracellular GSH synthesis by taking up cysteinyl glycine.
[Mh] Termos MeSH primário: Glutamatos/metabolismo
Glutationa/metabolismo
Proteína 1 Transportadora de Ânions Orgânicos/metabolismo
[Mh] Termos MeSH secundário: Ácidos Aminoipúricos/metabolismo
Ácido Aspártico/análogos & derivados
Ácido Aspártico/metabolismo
Transporte Biológico
Cisteína/metabolismo
Dipeptídeos/metabolismo
Estrona/análogos & derivados
Estrona/metabolismo
Glicina/metabolismo
Células HEK293
Seres Humanos
Rim/efeitos dos fármacos
Rim/metabolismo
Proteína 1 Transportadora de Ânions Orgânicos/genética
Transportadores de Ânions Orgânicos Sódio-Independentes/genética
Transportadores de Ânions Orgânicos Sódio-Independentes/metabolismo
Probenecid/farmacologia
Transfecção
Uricosúricos/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Aminohippuric Acids); 0 (Dipeptides); 0 (Glutamates); 0 (Organic Anion Transport Protein 1); 0 (Organic Anion Transporters, Sodium-Independent); 0 (Uricosuric Agents); 0 (organic anion transport protein 3); 2DI9HA706A (Estrone); 30KYC7MIAI (Aspartic Acid); 384644SZ9T (cysteinylglycine); GAN16C9B8O (Glutathione); K848JZ4886 (Cysteine); MA61H539YZ (N-acetylglutamic acid); PO572Z7917 (Probenecid); QTL48N278K (estrone sulfate); TE7660XO1C (Glycine)
[Em] Mês de entrada:1304
[Cu] Atualização por classe:131121
[Lr] Data última revisão:
131121
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:121221
[St] Status:MEDLINE
[do] DOI:10.1152/ajprenal.00412.2012


  2 / 1692 MEDLINE  
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[PMID]:17118613
[Au] Autor:Wang LH; Huang WS; Tai HM
[Ad] Endereço:Department of Applied Chemistry, Chia Nan University of Pharmacy and Science, Tainan 71743, Taiwan. e201466.wang@msa.hinet.net
[Ti] Título:Simultaneous determination of p-aminobenzoic acid and its metabolites in the urine of volunteers, treated with p-aminobenzoic acid sunscreen formulation.
[So] Source:J Pharm Biomed Anal;43(4):1430-6, 2007 Mar 12.
[Is] ISSN:0731-7085
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:p-Aminobenzoic acid (PABA) and its metabolites (p-aminohippuric acid, p-acetamidobenzoic acid, and p-acetamidohippuric acid) were detected using high-performance liquid chromatography with an electrochemical (carbon paste) detector (HPLC-ECD). For direct current (dc) mode, with the current at a constant potential, and measurements with suitable experimental parameters, a linear concentration from 0.125 to 1.80 microg/ml was found. The detection limit was approximately 2.0 ng/ml. A carbon paste coulometric detector was used to demonstrate that PABA and its metabolites are electrochemically oxidized in acidic media, and to determine, by analyzing human urine, the percutaneous absorption of PABA and its metabolites. Findings using HPLC-ECD and HPLC with an ultraviolet detector (HPLC-UV) were comparable.
[Mh] Termos MeSH primário: Ácido 4-Aminobenzoico/urina
Ácidos Aminoipúricos/urina
Protetores Solares/farmacocinética
Ácido p-Aminoipúrico/urina
para-Aminobenzoatos
[Mh] Termos MeSH secundário: Ácido 4-Aminobenzoico/farmacocinética
Adulto
Biotransformação
Cromatografia Líquida de Alta Pressão/métodos
Feminino
Seres Humanos
Masculino
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Aminohippuric Acids); 0 (Sunscreening Agents); 0 (para-Aminobenzoates); 04Z20NMK31 (Acedoben); 1984-38-9 (4-acetylaminohippuric acid); TL2TJE8QTX (4-Aminobenzoic Acid); Y79XT83BJ9 (p-Aminohippuric Acid)
[Em] Mês de entrada:0704
[Cu] Atualização por classe:141120
[Lr] Data última revisão:
141120
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:061123
[St] Status:MEDLINE


  3 / 1692 MEDLINE  
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[PMID]:17406486
[Au] Autor:Sentandreu MA; Toldrá F
[Ad] Endereço:Instituto de Agroquímica y Tecnología de Alimentos (CSIC), P.O. Box 73, 46100 Burjassot (Valencia), Spain. ciesen@iata.csic.es
[Ti] Título:A fluorescence-based protocol for quantifying angiotensin-converting enzyme activity.
[So] Source:Nat Protoc;1(5):2423-7, 2006.
[Is] ISSN:1750-2799
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The determination of angiotensin-converting enzyme (ACE) activity represents a useful tool in the study of different health pathologies, such as hypertension. This protocol describes a fluorescent assay for measuring ACE activity in vitro with high precision and sensitivity. The method relies on the ability of ACE to hydrolyse the internally quenched fluorescent substrate o-aminobenzoylglycyl-p-nitro-L-phenylalanyl-L-proline. The generation of the fluorescent product o-aminobenzoylglycine can be continuously monitored, preferably using a microtiter-plate fluorometer, though the use of a conventional cuvette fluorometer would also be possible. The method has important advantages with respect to other assays, because it involves only a one-step reagent, is easy to carry out and allows the analysis of an elevated number of samples in shorter times. It can be completed in one and a half hours. In addition, the fact that all reagents are commercially available allows the rapid introduction of the assay into the laboratory.
[Mh] Termos MeSH primário: Fluorometria/métodos
Peptidil Dipeptidase A/análise
[Mh] Termos MeSH secundário: Ácidos Aminoipúricos/análise
Inibidores da Enzima Conversora de Angiotensina/análise
Animais
Concentração Inibidora 50
Oligopeptídeos/metabolismo
Peptidil Dipeptidase A/metabolismo
Coelhos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Aminohippuric Acids); 0 (Angiotensin-Converting Enzyme Inhibitors); 0 (Oligopeptides); 526-21-6 (2-aminohippuric acid); 67482-93-3 (2-aminobenzoylglycyl-4-nitrophenylalanyl-proline); EC 3.4.15.1 (Peptidyl-Dipeptidase A)
[Em] Mês de entrada:0710
[Cu] Atualização por classe:080324
[Lr] Data última revisão:
080324
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:070405
[St] Status:MEDLINE


  4 / 1692 MEDLINE  
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[PMID]:16220119
[Au] Autor:Strickland JR; Custis MA; Ashley AK; Smith LL; Klotz JL; Krehbiel CR
[Ad] Endereço:Forage-Animal Production Research Unit, ARS, USDA, N-220F Ag Science North, Lexington, KY 40546, USA. jstrickland@ars.usda.gov
[Ti] Título:Clearance of para-aminohippuric acid in wethers consuming locoweed.
[So] Source:N Z Vet J;53(5):288-92, 2005 Oct.
[Is] ISSN:0048-0169
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:AIM: To validate the use of para-aminohippuric acid (PAH) as a marker for measuring blood flow in wethers consuming a mixed diet of locoweed and blue grama hay. METHODS: Fourteen sheep, stratified by bodyweight (BW), were assigned to one of three treatments: 0.8 mg swainsonine (SW)/kg BW (HI), 0.2 mg SW/kg BW (LO), and no SW (Control). Sheep were fed various ratios of locoweed and blue grama hay to deliver SW treatments, for 28 days prior to infusion of PAH. Concentrations of SW and activities of alkaline phosphatase (Alk-P) and aspartate aminotransferase (AST) in serum were measured to confirm exposure to SW and subclinical intoxication. A single 20-ml injection of 5% PAH was delivered into the jugular vein after subclinical intoxication had been achieved. Blood samples were collected and serum analysed for PAH immediately prior to injection, then every 5 min from 5-30 min, and every 10 min from 30-60 min, following injection of PAH. RESULTS: Effective delivery of SW was evident from the greater concentrations of SW measured in the serum of HI compared with LO animals (p<0.05). No significant differences were detected in the rate of elimination (range 0.097-0.108 L/min), elimination half-life (range 6.62-7.24 min), apparent volume of distribution for the central compartment (range 7.14-9.72 L), and clearance (range 0.73-0.92 L/min) of PAH, between treatments. CONCLUSIONS: Subclinical intoxication with SW did not affect the pharmacokinetics of PAH. Thus, use of downstream dilution of PAH is a valid method to determine the rate of blood flow in nutrient flux experiments that involve consumption of locoweed.
[Mh] Termos MeSH primário: Ácidos Aminoipúricos/farmacocinética
Velocidade do Fluxo Sanguíneo/veterinária
Inibidores Enzimáticos/farmacologia
Intoxicação por Plantas/veterinária
Doenças dos Ovinos/metabolismo
Swainsonina/farmacologia
[Mh] Termos MeSH secundário: Fosfatase Alcalina/metabolismo
Animais
Aspartato Aminotransferases/metabolismo
Velocidade do Fluxo Sanguíneo/efeitos dos fármacos
Relação Dose-Resposta a Droga
Fígado/enzimologia
Fígado/metabolismo
Masculino
Taxa de Depuração Metabólica/efeitos dos fármacos
Oxytropis/química
Oxytropis/toxicidade
Intoxicação por Plantas/enzimologia
Intoxicação por Plantas/metabolismo
Ovinos
Doenças dos Ovinos/enzimologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Nm] Nome de substância:
0 (Aminohippuric Acids); 0 (Enzyme Inhibitors); EC 2.6.1.1 (Aspartate Aminotransferases); EC 3.1.3.1 (Alkaline Phosphatase); RSY4RK37KQ (Swainsonine)
[Em] Mês de entrada:0601
[Cu] Atualização por classe:131121
[Lr] Data última revisão:
131121
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:051013
[St] Status:MEDLINE


  5 / 1692 MEDLINE  
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[PMID]:16169291
[Au] Autor:Farthing D; Sica DA; Fakhry I; Larus T; Ghosh S; Farthing C; Vranian M; Gehr T
[Ad] Endereço:Division of Nephrology, Clinical Pharmacology and Hypertension, VCU Medical Center, Virginia Commonwealth University, MCV Station, Box 980160, Richmond, VA 23298-0160, USA.
[Ti] Título:Simple HPLC-UV method for determination of iohexol, iothalamate, p-aminohippuric acid and n-acetyl-p-aminohippuric acid in human plasma and urine with ERPF, GFR and ERPF/GFR ratio determination using colorimetric analysis.
[So] Source:J Chromatogr B Analyt Technol Biomed Life Sci;826(1-2):267-72, 2005 Nov 05.
[Is] ISSN:1570-0232
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:A simple high-performance liquid chromatographic (HPLC) method was developed for the simultaneous determination of iohexol, iothalamate, p-aminohippuric acid (PAH) and n-acetyl-p-aminohippuric acid (n-acetyl-PAH) in human plasma and urine. A C(18) column at a flow rate of 1 ml/min with an aqueous mobile phase of trifluoroacetic acid (0.1% TFA in deionized water (pH 2.2), v/v) and methanol gradient was used for component separation. The plasma and urine assay demonstrated linearity from 10 to 50 microg/ml for iohexol and iothalamate, 5 to 40 microg/ml for PAH and 2.5 to 40 microg/ml for n-acetyl-PAH. The HPLC plasma and urine results obtained for PAH were used to calculate the subject kidney effective renal plasma flow (ERPF) and the iohexol results were used to calculate the subject kidney glomerular filtration rate (GFR). The HPLC results for PAH were then compared to an alternative colorimetric method for analyzing PAH to determine if subject metabolism (acetylation) of PAH affected the ERPF results obtained using the colorimetric method, the subsequent ERPF/GFR ratio and clinical impression of subject patient kidney function. The method was utilized in several different clinical studies evaluating the effect of kidney function from medications (phase IV evaluations) marketed for patients with cardiovascular disease.
[Mh] Termos MeSH primário: Ácidos Aminoipúricos/análise
Taxa de Filtração Glomerular/fisiologia
Iohexol/análise
Ácido Iotalâmico/análise
Fluxo Plasmático Renal Efetivo/fisiologia
Ácido p-Aminoipúrico/análise
[Mh] Termos MeSH secundário: Ácidos Aminoipúricos/sangue
Ácidos Aminoipúricos/urina
Cromatografia Líquida de Alta Pressão/métodos
Colorimetria/métodos
Seres Humanos
Reprodutibilidade dos Testes
Sensibilidade e Especificidade
Raios Ultravioleta
Ácido p-Aminoipúrico/sangue
Ácido p-Aminoipúrico/urina
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Aminohippuric Acids); 16CHD79MIX (Iothalamic Acid); 1984-38-9 (4-acetylaminohippuric acid); 4419T9MX03 (Iohexol); Y79XT83BJ9 (p-Aminohippuric Acid)
[Em] Mês de entrada:0512
[Cu] Atualização por classe:131121
[Lr] Data última revisão:
131121
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:050920
[St] Status:MEDLINE


  6 / 1692 MEDLINE  
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[PMID]:12380671
[Au] Autor:Magann EF; Whitworth NS; Files JC; Terrone DA; Chauhan SP; Morrison JC
[Ad] Endereço:Department of Obstetrics and Gynecology, University of Mississippi Medical Center, Jackson, USA.
[Ti] Título:Dye-dilution techniques using aminohippurate sodium: do they accurately reflect amniotic fluid volume?
[So] Source:J Matern Fetal Neonatal Med;11(3):167-70, 2002 Mar.
[Is] ISSN:1476-7058
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:OBJECTIVE: To determine whether the dye-dilution technique using aminohippurate sodium accurately measures amniotic fluid volume. METHODS: Singleton pregnancies with intact membranes undergoing a Cesarean delivery had their amniotic fluid volume assessed by the dye-dilution technique and direct measurement. RESULTS: Fifteen women were prospectively assessed. Six patients had their amniocentesis on the delivery table and nine patients at 4-24 h prior to the Cesarean delivery. The six women undergoing an amniocentesis just before delivery had good concordance between the dye-determined and direct measurement of amniotic fluid volume (r = 0.99, p = < 0.001). Among the nine women with varying times from amniocentesis to direct measurement, the correlation was not significant (r = 0.36, p = 0.08). The percentage difference between the dye-determined and directly measured amniotic fluid volume was significantly smaller in the women undergoing amniocentesis just prior to delivery (7%) than in the women with varying times from amniocentesis to delivery (37%, P < 0.001). CONCLUSION: Dye-determined amniotic fluid volume accurately reflects actual amniotic fluid volume but the dye-determined concentrations, in vivo, may undergo rapid changes.
[Mh] Termos MeSH primário: Ácidos Aminoipúricos
Líquido Amniótico/diagnóstico por imagem
Técnica de Diluição de Corante
Ultrassonografia Pré-Natal/métodos
[Mh] Termos MeSH secundário: Adolescente
Adulto
Amniocentese/métodos
Líquido Amniótico/fisiologia
Feminino
Seres Humanos
Gravidez
Estudos Prospectivos
Análise de Regressão
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Aminohippuric Acids)
[Em] Mês de entrada:0301
[Cu] Atualização por classe:161124
[Lr] Data última revisão:
161124
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:021017
[St] Status:MEDLINE


  7 / 1692 MEDLINE  
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[PMID]:11360056
[Au] Autor:Hemming AW; Gallinger S; Greig PD; Cattral MS; Langer B; Taylor BR; Verjee Z; Giesbrecht E; Nakamachi Y; Furuya KN
[Ad] Endereço:Department of Surgery, University of Florida, Gainesville 32610, USA. hemmiaw@mail.surgery.ufl.edu
[Ti] Título:The hippurate ratio as an indicator of functional hepatic reserve for resection of hepatocellular carcinoma in cirrhotic patients.
[So] Source:J Gastrointest Surg;5(3):316-21, 2001 May-Jun.
[Is] ISSN:1091-255X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Predicting the ability of the cirrhotic liver to withstand resection remains a challenge for the surgeon. This study evaluates the use of the hippurate ratio, a novel assessment of glycine conjugation of para-aminobenzoic acid by the liver, as a preoperative indicator of functional hepatic reserve. Between 1998 and 2000, sixty-one cirrhotic patients were prospectively assessed for hepatic resection using the hippurate ratio, indocyanine green retention at 15 minutes (ICG R-15), and other standard measures of liver function. Twenty-six patients were excluded as candidates for resection on the basis of inadequate functional hepatic reserve. Patients excluded from resection had significantly higher ICG R-15 values (29% +/- 9% vs. 16% +/- 12%, P = 0.001), higher Child-Pugh scores (5.9 +/- 0.9 vs. 5.3 +/- 0.4, P = 0.01), and lower hippurate ratios (30% +/- 14% vs. 45% +/- 15%, P = 0.005). There was a significant correlation between the hippurate ratio and ICG R-15. Other indicators of liver function such as factor V, factor VII, albumin, bilirubin, prothrombin time, and transaminases were no different between patients who did and those who did not undergo resection. Of the 35 patients resected, there were seven (20%) who developed varying degrees of liver failure with three perioperative deaths (8.5%). Patients who had some degree of liver failure had significantly lower hippurate ratios than patients who had no liver failure (29% +/- 10% vs. 48% +/- 14%, P = 0.002). There was no difference in ICG R-15 values between patients who had liver failure and those who did not. The hippurate ratio offers information on hepatocellular reserve that is not provided by other measures of liver function and may allow better selection of cirrhotic patients for liver resection.
[Mh] Termos MeSH primário: Ácido 4-Aminobenzoico/metabolismo
Carcinoma Hepatocelular/diagnóstico
Carcinoma Hepatocelular/metabolismo
Hepatectomia
Cirrose Hepática/diagnóstico
Cirrose Hepática/metabolismo
Falência Hepática/diagnóstico
Falência Hepática/metabolismo
Testes de Função Hepática/métodos
Neoplasias Hepáticas/diagnóstico
Neoplasias Hepáticas/metabolismo
Cuidados Pré-Operatórios/métodos
Ácido p-Aminoipúrico/sangue
[Mh] Termos MeSH secundário: Adulto
Idoso
Ácidos Aminoipúricos
Carcinoma Hepatocelular/complicações
Carcinoma Hepatocelular/cirurgia
Corantes
Feminino
Glicina/metabolismo
Hepatectomia/efeitos adversos
Hepatectomia/métodos
Hepatectomia/mortalidade
Seres Humanos
Verde de Indocianina
Cirrose Hepática/complicações
Falência Hepática/complicações
Testes de Função Hepática/normas
Neoplasias Hepáticas/complicações
Neoplasias Hepáticas/cirurgia
Masculino
Taxa de Depuração Metabólica
Meia-Idade
Cuidados Pré-Operatórios/normas
Estudos Prospectivos
Índice de Gravidade de Doença
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; VALIDATION STUDIES
[Nm] Nome de substância:
0 (Aminohippuric Acids); 0 (Coloring Agents); 1984-38-9 (4-acetylaminohippuric acid); IX6J1063HV (Indocyanine Green); TE7660XO1C (Glycine); TL2TJE8QTX (4-Aminobenzoic Acid); Y79XT83BJ9 (p-Aminohippuric Acid)
[Em] Mês de entrada:0108
[Cu] Atualização por classe:171104
[Lr] Data última revisão:
171104
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:010522
[St] Status:MEDLINE


  8 / 1692 MEDLINE  
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[PMID]:11135083
[Au] Autor:Dowling TC; Frye RF; Fraley DS; Matzke GR
[Ad] Endereço:Department of Pharmacy Practice and Science, School of Pharmacy, University of Maryland, Baltimore, Maryland, USA.
[Ti] Título:Characterization of tubular functional capacity in humans using para-aminohippurate and famotidine.
[So] Source:Kidney Int;59(1):295-303, 2001 Jan.
[Is] ISSN:0085-2538
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Renal drug excretion by glomerular filtration and active tubular secretion may be altered by factors such as acute and chronic renal disease, nephrotoxins, and drug interactions. Thus, accurate and reproducible methods for quantitation of glomerular filtration rate (GFR) and tubular functional capacity are critical. METHODS: We utilized a four-step sequential infusion method to characterize anionic [para-aminohippurate (PAH)] and cationic (famotidine) tubular functional capacity in healthy volunteers. Filtration and secretion rates were quantitated from renal clearance and iothalamate-derived GFR determinations. RESULTS: Concentration-dependent renal clearance of PAH was observed at plasma concentrations> 100 mg/L; renal clearances were 442 +/- 131 (mean +/- SD), 423 +/- 94, 233 +/- 45, and 152 +/- 18 mL/min/1.73 m2 at plasma concentrations of 18 +/- 2, 92 +/- 5, 291 +/- 47 and 789 +/- 28 mg/L, respectively. The apparent affinity (Km) and maximum secretory capacity (TmPAH) were 141 +/- 70 mg/L and 71 +/- 16 mg/min/1.73 m2, respectively. The unbound renal clearance and tubular secretory clearance of famotidine were 384 +/- 70 and 329 +/- 78 mL/min/1.73 m2, respectively, and were not significantly correlated with the unbound plasma concentrations, which ranged from 126 to 2659 ng/mL. The rate of tubular secretion was linear at unbound plasma concentrations up to 2659 ng/mL. CONCLUSIONS: These data indicate that a sequential infusion method using PAH may be used to characterize the anionic secretory component of proximal tubular function. The tubular clearance of famotidine may be a suitable index of the cationic secretory capacity of the proximal tubule in humans. Saturation of the cationic secretory pathway was not observed, and further investigation into parallel pathways of cationic secretion, such as p-glycoprotein, may be warranted.
[Mh] Termos MeSH primário: Famotidina
Túbulos Renais/fisiologia
Ácido p-Aminoipúrico
[Mh] Termos MeSH secundário: Adulto
Ácidos Aminoipúricos
Famotidina/farmacocinética
Seres Humanos
Masculino
Ácido p-Aminoipúrico/metabolismo
Ácido p-Aminoipúrico/farmacocinética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, U.S. GOV'T, P.H.S.
[Nm] Nome de substância:
0 (Aminohippuric Acids); 1984-38-9 (4-acetylaminohippuric acid); 5QZO15J2Z8 (Famotidine); Y79XT83BJ9 (p-Aminohippuric Acid)
[Em] Mês de entrada:0103
[Cu] Atualização por classe:151119
[Lr] Data última revisão:
151119
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:010103
[St] Status:MEDLINE


  9 / 1692 MEDLINE  
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[PMID]:10048113
[Au] Autor:Kim SY; Kim CH; Yoo HJ; Kim YK
[Ad] Endereço:Department of Pediatrics, College of Medicine, Pusan National University, Korea.
[Ti] Título:Effects of radical scavengers and antioxidant on ischemic acute renal failure in rabbits.
[So] Source:Ren Fail;21(1):1-11, 1999 Jan.
[Is] ISSN:0886-022X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:This study was undertaken to determine whether reactive oxygen species (ROS) are involved in the pathogenesis of ischemic acute renal failure (IARF) in rabbits. Renal ischemia was induced by clamping bilateral renal arteries for 60 min. Animals were pretreated with combination of xanthine oxidase inhibitor (allopurinol), hydrogen peroxide scavenger (catalase), and hydroxyl radical scavenger (sodium benzoate). Serum creatinine level significantly increased 24 h after ischemia and remained higher to 72 h. Ischemia caused a reduction of GFR and an increase of FENa. Such changes were significantly attenuated by scavenger pretreatment. The uptake of p-aminohippurate in cortical slices and microsomal Na(+)-K(+)-ATPase activity were depressed in kidneys subjected to 72 h of reflow following ischemia, indicating impairment of tubular transport function, which were significantly attenuated by scavenger treatment. Renal blood flow 72 h after reflow was markedly reduced and it was restored by scavenger pretreatment. When animals were pretreated with a potent antioxidant DPPD, lipid peroxidation in cortex and medulla was significantly inhibited. However, ischemia-induced impairment of renal function was not attenuated by pretreatment of the antioxidant. These results suggest that radical scavengers may exert a protective effect against ischemia acute renal failure by other actions rather than ROS scavenging. Thus, the data do not support involvement of ROS in IARF in rabbits.
[Mh] Termos MeSH primário: Lesão Renal Aguda/fisiopatologia
Antioxidantes/farmacologia
Espécies Reativas de Oxigênio/fisiologia
[Mh] Termos MeSH secundário: Lesão Renal Aguda/metabolismo
Adenosina Trifosfatases/metabolismo
Ácidos Aminoipúricos/metabolismo
Animais
Creatinina/sangue
Rim/efeitos dos fármacos
Rim/metabolismo
Rim/fisiopatologia
Peroxidação de Lipídeos/fisiologia
Masculino
Coelhos
Circulação Renal
Fatores de Tempo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Aminohippuric Acids); 0 (Antioxidants); 0 (Reactive Oxygen Species); AYI8EX34EU (Creatinine); EC 3.6.1.- (Adenosine Triphosphatases)
[Em] Mês de entrada:9907
[Cu] Atualização por classe:131121
[Lr] Data última revisão:
131121
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:990227
[St] Status:MEDLINE


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[PMID]:9824245
[Au] Autor:Dowling TC; Frye RF; Zemaitis MA
[Ad] Endereço:Department of Pharmaceutical Sciences, School of Pharmacy, University of Pittsburgh, PA 15261, USA.
[Ti] Título:Simultaneous determination of p-aminohippuric acid, acetyl-p-aminohippuric acid and iothalamate in human plasma and urine by high-performance liquid chromatography.
[So] Source:J Chromatogr B Biomed Sci Appl;716(1-2):305-13, 1998 Sep 25.
[Is] ISSN:1387-2273
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:A sensitive and specific high-performance liquid chromatographic assay was developed for the simultaneous determination of p-aminohippuric acid (PAH), acetyl-p-aminohippuric acid (aPAH), and iothalamate in human plasma and urine. Plasma samples were prepared by protein precipitation with acetonitrile followed by evaporation, reconstitution in mobile phase, and injection onto a C18 reversed-phase column. Urine samples were diluted with 3 volumes of mobile phase prior to injection. Column effluent was monitored by UV detection at 254 nm. The lower limits of quantification in plasma were 0.5 mg/l for PAH and aPAH, and 1.0 mg/l for iothalamate. The within-day and between-day coefficients of variation in plasma and urine were < or =7.8% for all analytes. This method is well suited for renal function studies using iothalamate and PAH, whether administered as a bolus dose or by continuous infusion, to measure glomerular filtration rate and effective renal plasma flow, respectively.
[Mh] Termos MeSH primário: Ácidos Aminoipúricos
Cromatografia Líquida de Alta Pressão/métodos
Meios de Contraste
Ácido Iotalâmico/análise
Ácido p-Aminoipúrico/análise
[Mh] Termos MeSH secundário: Acetonitrilos
Proteínas Sanguíneas
Precipitação Química
Taxa de Filtração Glomerular
Seres Humanos
Controle de Qualidade
Circulação Renal
Sensibilidade e Especificidade
Ácido p-Aminoipúrico/sangue
Ácido p-Aminoipúrico/urina
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, U.S. GOV'T, P.H.S.
[Nm] Nome de substância:
0 (Acetonitriles); 0 (Aminohippuric Acids); 0 (Blood Proteins); 0 (Contrast Media); 16CHD79MIX (Iothalamic Acid); 1984-38-9 (4-acetylaminohippuric acid); Y79XT83BJ9 (p-Aminohippuric Acid); Z072SB282N (acetonitrile)
[Em] Mês de entrada:9902
[Cu] Atualização por classe:131121
[Lr] Data última revisão:
131121
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:981121
[St] Status:MEDLINE



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