[PMID]: | 28062355 |
[Au] Autor: | Nishimura H; Yamaguchi D; Watanabe T |
[Ad] Endereço: | Laboratory of Biomass Conversion, Research Institute for Sustainable Humanosphere (RISH), Kyoto University, Gokasho, Uji, Kyoto, Japan. Electronic address: hiroshi_nishimura@rish.kyoto-u.ac.jp. |
[Ti] Título: | Cerebrosides, extracellular glycolipids secreted by the selective lignin-degrading fungus Ceriporiopsis subvermispora. |
[So] Source: | Chem Phys Lipids;203:1-11, 2017 Mar. |
[Is] ISSN: | 1873-2941 |
[Cp] País de publicação: | Ireland |
[La] Idioma: | eng |
[Ab] Resumo: | Ceriporiopsis subvermispora is a selective white-rot fungus that degrades lignin at a site far from the hyphae and extracellular enzymes, without intensive damage to the cellulose. In selective ligninolysis, low molecular mass metabolites play a principal role and amphipathic substances are involved to control the degradation and transport of hydrophobic aromatic molecules, including lignin and lipids; however, secretion of the amphipathic substances by this fungus has not been well understood, except for alk(en)yl itaconates called ceriporic acids, which have a weak amphiphilicity. Herein, we report for the first time that the fungus secretes cerebrosides that are classified as glycosphingolipids. By using liquid chromatography electron spray ionization mass spectrometry (LC-ESI-MS) and nuclear magnetic resonance (NMR) spectroscopy coupled with stable isotope feeding experiments with C-glucose and N-ammonium sulfate, the cerebrosides were determined to be N-hydroxyoctadecanoyl-1-O-ß-d-glucopyranosyl-4E,8E-sphingadienine, N-hydroxyoctadecanoyl-1-O-ß-d-glucopyranosyl-4E,8Z-sphingadienine, and N-hydroxyoctadecanoyl-1-O-ß-d-glucopyranosyl-9-methyl-4E,8E-sphingadienine. The cerebrosides are strong amphipathic substances and potential metabolites for regulating difference and symbiosis within the microbial community. |
[Mh] Termos MeSH primário: |
Cerebrosídeos/metabolismo Coriolaceae/metabolismo Lignina/metabolismo
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[Mh] Termos MeSH secundário: |
Cerebrosídeos/química Lignina/química Estrutura Molecular
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[Pt] Tipo de publicação: | JOURNAL ARTICLE |
[Nm] Nome de substância:
| 0 (Cerebrosides); 9005-53-2 (Lignin) |
[Em] Mês de entrada: | 1705 |
[Cu] Atualização por classe: | 170502 |
[Lr] Data última revisão:
| 170502 |
[Sb] Subgrupo de revista: | IM |
[Da] Data de entrada para processamento: | 170108 |
[St] Status: | MEDLINE |
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