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[PMID]: 28778013 [Au] Autor: Yu JS; Moon E; Kim KH [Ad] Endereço: School of Pharmacy, Sungkyunkwan University, Suwon 440-746, Republic of Korea. [Ti] Título: A new cerebroside from the twigs of Lindera glauca (Sieb. et Zucc.) Blume. [So] Source: Bioorg Chem;74:122-125, 2017 Oct. [Is] ISSN: 1090-2120 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: Lindera glauca (Sieb. et Zucc.) Blume (Lauraceae) has been used to treat rheumatic arthritis, stroke, and cardiac pain. Phytochemical investigation of twigs of L. glauca (Sieb. et Zucc.) Blume resulted in the isolation and identification of a new cerebroside, glaucerebroside (1). The structure of 1 was elucidated by a combination of extensive spectroscopic analyses, including extensive 2D NMR, HR-MS, chemical reactions, and LC/MS analysis. Compound 1 is a relatively rare cerebroside with l-threo-configuration of the sphingosine part. This is the second example of identification of a cerebroside from the family Lauraceae. Compound 1 significantly inhibited nitric oxide (NO) production in lipopolysaccharide (LPS)-stimulated BV-2 cells, with an IC value of 23.84µM without inducing cell toxicity. This study suggests that glaucerebroside (1) can be an excellent candidate for development of novel anti-neuroinflammatory agents. [Mh] Termos MeSH primário: Cerebrosídeos/farmacologia Lindera/química Caules de Planta/química [Mh] Termos MeSH secundário: Animais Sobrevivência Celular/efeitos dos fármacos Cerebrosídeos/química Cerebrosídeos/isolamento & purificação Relação Dose-Resposta a Droga Lipopolissacarídeos/antagonistas & inibidores Lipopolissacarídeos/farmacologia Camundongos Conformação Molecular Óxido Nítrico/antagonistas & inibidores Óxido Nítrico/biossíntese Relação Estrutura-Atividade [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Cerebrosides); 0 (Lipopolysaccharides); 31C4KY9ESH (Nitric Oxide) [Em] Mês de entrada: 1710 [Cu] Atualização por classe: 171010 [Lr] Data última revisão: 171010 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170805 [St] Status: MEDLINE

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[PMID]: 28406733 [Au] Autor: Che H; Du L; Cong P; Tao S; Ding N; Wu F; Xue C; Xu J; Wang Y [Ad] Endereço: 1 College of Food Science and Engineering, Ocean University of China , Qingdao, China . [Ti] Título: Cerebrosides from Sea Cucumber Protect Against Oxidative Stress in SAMP8 Mice and PC12 Cells. [So] Source: J Med Food;20(4):392-402, 2017 Apr. [Is] ISSN: 1557-7600 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: Alzheimer's disease (AD) is a neurodegenerative disorder. Emerging evidence implicates ß-amyloid (Aß) plays a critical role in the progression of AD. In this study, we investigated the protective effect of cerebrosides obtained from sea cucumber against senescence-accelerated mouse prone 8 (SAMP8) mice in vivo. We also studied the effect of cerebrosides on Aß-induced cytotoxicity on the rat pheochromocytoma cell (PC12) and the underlying molecular mechanisms. Cerebrosides ameliorated learning and memory deficits and the Aß accumulation in demented mice, decreased the content of malondialdehyde (MDA), 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-OHdG), 8-hydroxy-2'-deoxyguanosine (8-oxo-G), and nitric oxide (NO), and enhanced the superoxide dismutase (SOD) activity significantly. The neuroprotective effect of sea cucumber cerebrosides (SCC) was also verified in vitro: the cerebrosides increased the survival rate of PC12 cells, recovered the cellular morphology, downregulated the protein levels of Caspase-9, cleaved Caspase-3, total Caspase-3, and Bax, and upregulated the protein level of Bcl-2, revealing that cerebrosides could inhibit Aß-induced cell apoptosis. The results showed the protective effect of SCC was regulated by the mitochondria-dependent apoptotic pathway. Our results provide a new approach to developing the marine organisms as functional foods for neuroprotection. [Mh] Termos MeSH primário: Cerebrosídeos/farmacologia Fármacos Neuroprotetores/farmacologia Estresse Oxidativo/efeitos dos fármacos Pepinos-do-Mar/química [Mh] Termos MeSH secundário: Doença de Alzheimer Peptídeos beta-Amiloides/química Animais Apoptose Sobrevivência Celular Guanosina/química Hipocampo/química Aprendizagem Masculino Malondialdeído/química Transtornos da Memória Camundongos Camundongos Endogâmicos Óxido Nítrico/química Células PC12 Ratos Superóxido Dismutase/metabolismo [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Amyloid beta-Peptides); 0 (Cerebrosides); 0 (Neuroprotective Agents); 12133JR80S (Guanosine); 31C4KY9ESH (Nitric Oxide); 4Y8F71G49Q (Malondialdehyde); EC 1.15.1.1 (Superoxide Dismutase) [Em] Mês de entrada: 1705 [Cu] Atualização por classe: 170515 [Lr] Data última revisão: 170515 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170414 [St] Status: MEDLINE [do] DOI: 10.1089/jmf.2016.3789

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[PMID]: 28394604 [Au] Autor: Jin Y; Fan JT; Gu XL; Zhang LY; Han J; Du SH; Zhang AX [Ti] Título: Neuroprotective Activity of Cerebrosides from Typhonium giganteum by Regulating Caspase-3 and Bax/Bcl-2 Signaling Pathways in PC12 Cells. [So] Source: J Nat Prod;80(6):1734-1741, 2017 Jun 23. [Is] ISSN: 1520-6025 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: An investigation of the potential neuroprotective natural product constituents of the rhizomes of Typhonium giganteum led to the isolation of two new cerebrosides, typhonosides E (1) and F (2), along with 11 known analogues (3-13). The structures of compounds 1 and 2 were elucidated by spectroscopic data interpretation. The activity of these compounds against glutamate-induced cell apoptosis was investigated in PC12 cells. All compounds exhibited such activity, which was related to the length of the fatty acyl chain. Among them, longan cerebroside II (11), with the longest fatty acyl chain, showed the most potent protective effect in PC12 cells from glutamate injury, with an EC value of 2.5 µM. Moreover, at the molecular level, longan cerebroside II (11) downregulated the expression of caspase-9, caspase-3, and Bax, upregulated the expression of Bcl-2, and decreased the level of cytosolic cytochrome c in a concentration-dependent manner. [Mh] Termos MeSH primário: Cerebrosídeos/isolamento & purificação Cerebrosídeos/farmacologia Fármacos Neuroprotetores/isolamento & purificação Fármacos Neuroprotetores/farmacologia [Mh] Termos MeSH secundário: Animais Apoptose/efeitos dos fármacos Caspase 3/metabolismo Caspase 9/metabolismo Cerebrosídeos/química Citocromos c/metabolismo Ácido Glutâmico/farmacologia Estrutura Molecular Fármacos Neuroprotetores/química Células PC12 Proteínas Proto-Oncogênicas c-bcl-2/metabolismo Ratos Rizoma/química Sapindaceae Transdução de Sinais/efeitos dos fármacos [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Cerebrosides); 0 (Neuroprotective Agents); 0 (Proto-Oncogene Proteins c-bcl-2); 0 (typhonoside); 0 (typhonoside E); 0 (typhonoside F); 3KX376GY7L (Glutamic Acid); 9007-43-6 (Cytochromes c); EC 3.4.22.- (Caspase 3); EC 3.4.22.- (Caspase 9) [Em] Mês de entrada: 1708 [Cu] Atualização por classe: 170830 [Lr] Data última revisão: 170830 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170411 [St] Status: MEDLINE [do] DOI: 10.1021/acs.jnatprod.6b00954

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[PMID]: 28062355 [Au] Autor: Nishimura H; Yamaguchi D; Watanabe T [Ad] Endereço: Laboratory of Biomass Conversion, Research Institute for Sustainable Humanosphere (RISH), Kyoto University, Gokasho, Uji, Kyoto, Japan. Electronic address: hiroshi_nishimura@rish.kyoto-u.ac.jp. [Ti] Título: Cerebrosides, extracellular glycolipids secreted by the selective lignin-degrading fungus Ceriporiopsis subvermispora. [So] Source: Chem Phys Lipids;203:1-11, 2017 Mar. [Is] ISSN: 1873-2941 [Cp] País de publicação: Ireland [La] Idioma: eng [Ab] Resumo: Ceriporiopsis subvermispora is a selective white-rot fungus that degrades lignin at a site far from the hyphae and extracellular enzymes, without intensive damage to the cellulose. In selective ligninolysis, low molecular mass metabolites play a principal role and amphipathic substances are involved to control the degradation and transport of hydrophobic aromatic molecules, including lignin and lipids; however, secretion of the amphipathic substances by this fungus has not been well understood, except for alk(en)yl itaconates called ceriporic acids, which have a weak amphiphilicity. Herein, we report for the first time that the fungus secretes cerebrosides that are classified as glycosphingolipids. By using liquid chromatography electron spray ionization mass spectrometry (LC-ESI-MS) and nuclear magnetic resonance (NMR) spectroscopy coupled with stable isotope feeding experiments with C-glucose and N-ammonium sulfate, the cerebrosides were determined to be N-hydroxyoctadecanoyl-1-O-ß-d-glucopyranosyl-4E,8E-sphingadienine, N-hydroxyoctadecanoyl-1-O-ß-d-glucopyranosyl-4E,8Z-sphingadienine, and N-hydroxyoctadecanoyl-1-O-ß-d-glucopyranosyl-9-methyl-4E,8E-sphingadienine. The cerebrosides are strong amphipathic substances and potential metabolites for regulating difference and symbiosis within the microbial community. [Mh] Termos MeSH primário: Cerebrosídeos/metabolismo Coriolaceae/metabolismo Lignina/metabolismo [Mh] Termos MeSH secundário: Cerebrosídeos/química Lignina/química Estrutura Molecular [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Cerebrosides); 9005-53-2 (Lignin) [Em] Mês de entrada: 1705 [Cu] Atualização por classe: 170502 [Lr] Data última revisão: 170502 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170108 [St] Status: MEDLINE

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[PMID]: 27868429 [Au] Autor: McCloskey S; Noppawan S; Mongkolthanaruk W; Suwannasai N; Senawong T; Prawat U [Ad] Endereço: a Natural Products Research Unit, Faculty of Science, Department of Chemistry , Khon Kaen University, Centre of Excellence for Innovation in Chemistry (PERCH-CIC) , Khon Kaen , Thailand. [Ti] Título: A new cerebroside and the cytotoxic constituents isolated from Xylaria allantoidea SWUF76. [So] Source: Nat Prod Res;31(12):1422-1430, 2017 Jun. [Is] ISSN: 1478-6427 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: A new cerebroside, namely allantoside (1), and 10 known compounds (2-11) were isolated from Xylaria allantoidea SWUF76. The structure of compound 1 was determined by comprehensive spectroscopic analysis including 1D and 2D nuclear magnetic resonance (NMR) as well as high-resolution electron ionisation mass spectrometry (HREIMS) and electrospray ionisation mass spectrometry (ESIMS). Compounds 1, 4, 5, 6, 7, 8 and 11 were evaluated for cytotoxic activities against cancer cell lines (Hela, HT29, HCT116 and MCF-7) and normal Vero cell lines by MTT assay. Compounds 6 and 7 exhibited anticancer activity after 24 h of treatment. Compound 7 showed significant cytotoxicity against Hela (IC = 2.24 µg/mL), HT29 (IC = 2.51 µg/mL), HCT116 (IC = 3.50 µg/mL) and MCF-7 (IC = 3.77 µg/mL) and Vero (IC :3.65 µg/mL) cells. Compound 6 showed slight cytotoxicity against all tested cancer cell lines. [Mh] Termos MeSH primário: Antineoplásicos/isolamento & purificação Cerebrosídeos/isolamento & purificação Xylariales/metabolismo [Mh] Termos MeSH secundário: Antineoplásicos/química Antineoplásicos/farmacologia Linhagem Celular Tumoral Cerebrosídeos/química Cerebrosídeos/farmacologia Fermentação Seres Humanos Espectroscopia de Ressonância Magnética [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Antineoplastic Agents); 0 (Cerebrosides) [Em] Mês de entrada: 1706 [Cu] Atualização por classe: 170609 [Lr] Data última revisão: 170609 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 161122 [St] Status: MEDLINE [do] DOI: 10.1080/14786419.2016.1258559

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[PMID]: 27585906 [Au] Autor: Duan J; Ishida M; Aida K; Tsuduki T; Zhang J; Manabe Y; Hirata T; Sugawara T [Ad] Endereço: Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University , Kyoto 606-8502, Japan. [Ti] Título: Dietary Cerebroside from Sea Cucumber (Stichopus japonicus): Absorption and Effects on Skin Barrier and Cecal Short-Chain Fatty Acids. [So] Source: J Agric Food Chem;64(37):7014-21, 2016 Sep 21. [Is] ISSN: 1520-5118 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: Sphingolipids from marine sources have attracted more attention recently because of their distinctive structures and expected functions. In this study, the content and components of cerebroside from sea cucumber Stichopus japonicus were analyzed. The absorption of cerebroside from S. japonicus was investigated with an in vivo lipid absorption assay. The result revealed that S. japonicus is a rich source of cerebroside that contained considerable amounts of odd carbon chain sphingoid bases. The cumulative recoveries of d17:1- and d19:2-containing cerebrosides were 0.31 ± 0.16 and 0.32 ± 0.10%, respectively, for 24 h after administration. To the best of the authors' knowledge, this is the first work that shows sphingolipids from a marine source could be absorbed in vivo and incorporated into ceramides. In addition, dietary supplementation with sea cucumber cerebroside to hairless mouse improved the skin barrier function and increased short-chain fatty acids in cecal contents, which have shown beneficial effects on the host. [Mh] Termos MeSH primário: Ceco/metabolismo Cerebrosídeos/metabolismo Ácidos Graxos Voláteis/metabolismo Pele/metabolismo Stichopus/química [Mh] Termos MeSH secundário: Animais Ceco/química Cerebrosídeos/química Suplementos Nutricionais/análise Ácidos Graxos Voláteis/química Feminino Masculino Camundongos Camundongos Pelados Ratos Ratos Sprague-Dawley Pele/química [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Cerebrosides); 0 (Fatty Acids, Volatile) [Em] Mês de entrada: 1704 [Cu] Atualização por classe: 170414 [Lr] Data última revisão: 170414 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 160903 [St] Status: MEDLINE [do] DOI: 10.1021/acs.jafc.6b02564

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[PMID]: 27005610 [Au] Autor: Youssef DT; Ibrahim SR; Shaala LA; Mohamed GA; Banjar ZM [Ad] Endereço: Department of Natural Products, Faculty of Pharmacy, King Abdulaziz University, Jeddah 21589, Saudi Arabia. dyoussef@kau.edu.sa. [Ti] Título: New Cerebroside and Nucleoside Derivatives from a Red Sea Strain of the Marine Cyanobacterium Moorea producens. [So] Source: Molecules;21(3):324, 2016 Mar 09. [Is] ISSN: 1420-3049 [Cp] País de publicação: Switzerland [La] Idioma: eng [Ab] Resumo: In the course of our ongoing efforts to identify marine-derived bioactive compounds, the marine cyanobacterium Moorea producens was investigated. The organic extract of the Red Sea cyanobacterium afforded one new cerebroside, mooreaside A (1), two new nucleoside derivatives, 3-acetyl-2'-deoxyuridine (2) and 3-phenylethyl-2'-deoxyuridine (3), along with the previously reported compounds thymidine (4) and 2,3-dihydroxypropyl heptacosanoate (5). The structures of the compounds were determined by different spectroscopic studies (UV, IR, 1D, 2D NMR, and HRESIMS), as well as comparison with the literature data. Compounds 1-5 showed variable cytotoxic activity against three cancer cell lines. [Mh] Termos MeSH primário: Cerebrosídeos/farmacologia Cianobactérias/química Neoplasias/tratamento farmacológico Nucleosídeos/farmacologia [Mh] Termos MeSH secundário: Linhagem Celular Tumoral Cerebrosídeos/química Cerebrosídeos/isolamento & purificação Seres Humanos Oceano Índico Estrutura Molecular Nucleosídeos/química Nucleosídeos/isolamento & purificação [Pt] Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T [Nm] Nome de substância: 0 (Cerebrosides); 0 (Nucleosides) [Em] Mês de entrada: 1612 [Cu] Atualização por classe: 161230 [Lr] Data última revisão: 161230 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 160324 [St] Status: MEDLINE

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[PMID]: 27001385 [Au] Autor: Du L; Yang YH; Xu J; Wang YM; Xue CH; Kurihara H; Takahashi K [Ad] Endereço: Faculty of Fisheries Sciences, Hokkaido University, 3-1-1 Minato-cho, Hakodate, Hokkaido 041-8611, Japan. kore@fish.hokudai.ac.jp. [Ti] Título: Transport and uptake effects of marine complex lipid liposomes in small intestinal epithelial cell models. [So] Source: Food Funct;7(4):1904-14, 2016 Apr. [Is] ISSN: 2042-650X [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: Nowadays, marine complex lipids, including starfish phospholipids (SFP) and cerebrosides (SFC) separated from Asterias amurensis as well as sea cucumber phospholipids (SCP) and cerebrosides (SCC) isolated from Cucumaria frondosa, have received much attention because of their potent biological activities. However, little information is known on the transport and uptake of these lipids in liposome forms in small intestinal cells. Therefore, this study was undertaken to investigate the effects of these complex lipid liposomes on transport and uptake in Caco-2 and M cell monolayer models. The results revealed that SFP and SCP contained 42% and 47.9% eicosapentaenoic acid (EPA), respectively. The average particle sizes of liposomes prepared in this study were from 169 to 189 nm. We found that the transport of the liposomes across the M cell monolayer model was much higher than the Caco-2 cell monolayer model. The liposomes consisting of SFP or SCP showed significantly higher transport and uptake than soy phospholipid (soy-PL) liposomes in both Caco-2 and M cell monolayer models. Our results also exhibited that treatment with 1 mM liposomes composed of SFP or SCP for 3 h tended to increase the EPA content in phospholipid fractions of both differentiated Caco-2 and M cells. Moreover, it was also found that the hybrid liposomes consisting of SFP/SFC/cholesterol (Chol) revealed higher transport and uptake across the M cell monolayer in comparison with other liposomes. Furthermore, treatment with SFP/SFC/Chol liposomes could notably decrease the trans-epithelial electrical resistance (TEER) values of Caco-2 and M cell monolayers. The present data also showed that the cell viability of differentiated Caco-2 and M cells was not affected after the treatment with marine complex lipids or soy-PL liposomes. Based on the data in this study, it was suggested that marine complex lipid liposomes exhibit prominent transport and uptake in small intestinal epithelial cell models. [Mh] Termos MeSH primário: Asterias/química Cerebrosídeos/metabolismo Cucumaria/química Células Epiteliais/metabolismo Intestino Delgado/metabolismo Lipossomos/metabolismo Fosfolipídeos/metabolismo Alimentos Marinhos/análise [Mh] Termos MeSH secundário: Animais Transporte Biológico Células CACO-2 Sobrevivência Celular Cerebrosídeos/química Seres Humanos Lipossomos/química Modelos Biológicos Fosfolipídeos/química [Pt] Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T [Nm] Nome de substância: 0 (Cerebrosides); 0 (Liposomes); 0 (Phospholipids) [Em] Mês de entrada: 1701 [Cu] Atualização por classe: 170110 [Lr] Data última revisão: 170110 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 160323 [St] Status: MEDLINE [do] DOI: 10.1039/c6fo00066e

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[PMID]: 26853111 [Au] Autor: Chiu CP; Liu SC; Tang CH; Chan Y; El-Shazly M; Lee CL; Du YC; Wu TY; Chang FR; Wu YC [Ad] Endereço: Graduate Institute of Natural Products, College of Pharmacy, Kaohsiung Medical University , Kaohsiung 807, Taiwan. [Ti] Título: Anti-inflammatory Cerebrosides from Cultivated Cordyceps militaris. [So] Source: J Agric Food Chem;64(7):1540-8, 2016 Feb 24. [Is] ISSN: 1520-5118 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: Cordyceps militaris (bei-chong-chaw, northern worm grass) is a precious and edible entomopathogenic fungus, which is widely used in traditional Chinese medicine (TCM) as a general booster for the nervous system, metabolism, and immunity. Saccharides, nucleosides, mannitol, and sterols were isolated from this fungus. The biological activity of C. militaris was attributed to the saccharide and nucleoside contents. In this study, the aqueous methanolic fraction of C. militaris fruiting bodies exhibited a significant anti-inflammatory activity. Bioactivity-guided fractionation of the active fraction led to the isolation of eight compounds, including one new and two known cerebrosides (ceramide derivatives), two nucleosides, and three sterols. Cordycerebroside A (1), the new cerebroside, along with soyacerebroside I (2) and glucocerebroside (3) inhibited the accumulation of pro-inflammatory iNOS protein and reduced the expression of COX-2 protein in LPS-stimulated RAW264.7 macrophages. This is the first study on the isolation of cerebrosides with anti-inflammatory activity from this TCM. [Mh] Termos MeSH primário: Anti-Inflamatórios/química Anti-Inflamatórios/farmacologia Cerebrosídeos/química Cerebrosídeos/farmacologia Cordyceps/química [Mh] Termos MeSH secundário: Animais Anti-Inflamatórios/isolamento & purificação Cerebrosídeos/isolamento & purificação Cordyceps/crescimento & desenvolvimento Carpóforos/química Carpóforos/crescimento & desenvolvimento Macrófagos/efeitos dos fármacos Macrófagos/imunologia Camundongos Óxido Nítrico Sintase Tipo II/genética Óxido Nítrico Sintase Tipo II/imunologia Células RAW 264.7 [Pt] Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T [Nm] Nome de substância: 0 (Anti-Inflammatory Agents); 0 (Cerebrosides); EC 1.14.13.39 (Nitric Oxide Synthase Type II); EC 1.14.13.39 (Nos2 protein, mouse) [Em] Mês de entrada: 1610 [Cu] Atualização por classe: 161230 [Lr] Data última revisão: 161230 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 160209 [St] Status: MEDLINE [do] DOI: 10.1021/acs.jafc.5b05931

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[PMID]: 26823708 [Au] Autor: Ye Q; Zhang N; Chen K; Zhu J; Jiang H [Ad] Endereço: Department of Hematology, Shanghai Children's Hospital, Shanghai Jiao Tong University China. [Ti] Título: Effects of portulacerebroside a on apoptosis of human leukemia HL60 cells and p38/JNK signaling pathway. [So] Source: Int J Clin Exp Pathol;8(11):13968-77, 2015. [Is] ISSN: 1936-2625 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: Acute myeloid leukemia is known as one of the most malignant diseases. We aimed at exploring the effect of portulacerebroside A (PCA) on the apoptosis in human leukemia HL60 cells and clarify the possible mechanisms involved in. By MTT analysis, we found that PCA (1-100 µM) inhibited the cell viability in a time- and dose-dependent manner, and cell cycle was arrested at G0/G1 period. PCA treatment from 5 to 50 µM dose-dependently induced apoptosis from 12.7 ± 1.56% to 52.7 ± 6.214% of HL60 cells. Mitochondrial membrane potential (MMP) was decreased and reactive oxygen species (ROS) accumulated obviously. mRNA expressions and protein levels of Bax/Bcl-2, caspase-3 and caspase-9 were elevated significantly. ERK1/2, JNK1/2 and p38 MAPK pathway were blocked detected by western blot analysis. In conclusion, PCA can act as a new agent for leucocythemia treatment. [Mh] Termos MeSH primário: Antineoplásicos Fitogênicos/farmacologia Apoptose/efeitos dos fármacos Cerebrosídeos/farmacologia Glucosilceramidas/farmacologia Proteínas Quinases JNK Ativadas por Mitógeno/metabolismo Leucemia Mieloide Aguda/tratamento farmacológico Proteínas Quinases p38 Ativadas por Mitógeno/metabolismo [Mh] Termos MeSH secundário: Antineoplásicos Fitogênicos/isolamento & purificação Apoptose/genética Proteínas Reguladoras de Apoptose/genética Proteínas Reguladoras de Apoptose/metabolismo Pontos de Checagem do Ciclo Celular/efeitos dos fármacos Proliferação Celular/efeitos dos fármacos Sobrevivência Celular/efeitos dos fármacos Cerebrosídeos/isolamento & purificação Relação Dose-Resposta a Droga Regulação para Baixo Regulação Neoplásica da Expressão Gênica Glucosilceramidas/isolamento & purificação Células HL-60 Seres Humanos Leucemia Mieloide Aguda/enzimologia Leucemia Mieloide Aguda/genética Leucemia Mieloide Aguda/patologia Potencial da Membrana Mitocondrial/efeitos dos fármacos Mitocôndrias/efeitos dos fármacos Mitocôndrias/metabolismo Mitocôndrias/patologia Fosforilação Portulaca/química Espécies Reativas de Oxigênio/metabolismo Transdução de Sinais/efeitos dos fármacos Fatores de Tempo [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Antineoplastic Agents, Phytogenic); 0 (Apoptosis Regulatory Proteins); 0 (Cerebrosides); 0 (Glucosylceramides); 0 (Reactive Oxygen Species); 0 (portulacerebroside A); EC 2.7.11.24 (JNK Mitogen-Activated Protein Kinases); EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases) [Em] Mês de entrada: 1610 [Cu] Atualização por classe: 170220 [Lr] Data última revisão: 170220 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 160130 [St] Status: MEDLINE

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1			PalavrasDescritor de assuntoDescritor de assunto primárioLimitesAutorPalavras do títuloPalavras do resumoIdiomaPaís de publicaçãoNome de substânciaNome de pessoa como assuntoRevistaAssunto de RevistaISSNTipo de publicaçãoIdentificador únicoMês de entradaAno de publicaçãoTexto completoAtualização por classeCategoria DeCSCategoria DeCS explodidaCategoria CID-10SubSetsPais de AfiliaçãoAfiliaçãoAfiliação 2
2	and or and not		PalavrasDescritor de assuntoDescritor de assunto primárioLimitesAutorPalavras do títuloPalavras do resumoIdiomaPaís de publicaçãoNome de substânciaNome de pessoa como assuntoRevistaAssunto de RevistaISSNTipo de publicaçãoIdentificador únicoMês de entradaAno de publicaçãoTexto completoAtualização por classeCategoria DeCSCategoria DeCS explodidaCategoria CID-10SubSetsPais de AfiliaçãoAfiliaçãoAfiliação 2
3	and or and not		PalavrasDescritor de assuntoDescritor de assunto primárioLimitesAutorPalavras do títuloPalavras do resumoIdiomaPaís de publicaçãoNome de substânciaNome de pessoa como assuntoRevistaAssunto de RevistaISSNTipo de publicaçãoIdentificador únicoMês de entradaAno de publicaçãoTexto completoAtualização por classeCategoria DeCSCategoria DeCS explodidaCategoria CID-10SubSetsPais de AfiliaçãoAfiliaçãoAfiliação 2

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